Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0005823

Physical Location & Seq

Genomic Coordinates Forward (+)

2696381 .. 2701022

Gene Structure 4642 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G08520.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2283 bp

ATGGCGATGACTCCGGTCGCGTCATCATCTCCAGTTTCAACCTGCAGACTCTTTCGCTGCAATCTCCTCCCTGATCTCTTACCTAAGCCTCTGTTTCTCTCCCTCCCCAAACGAAACAGAATTGCCTCGTGCCGCTTCACTGTACGTGCCTCCGCGAATGCTACCGTCGAATCCCCTAACGGTGTCCCTGCCTCCACATCAGATACGGATACGGAGACGGATACCACCTCCTATGGCCGACAGTTTTTCCCTTTGGCCGCAGTTGTTGGCCAGGAAGGCATAAAAACTGCTCTTTTACTTGGCGCGGTTGATCGTGAAATCGGAGGGATTGCCATTTCAGGTCGTAGAGGCACTGCAAAAACAGTCATGGCGCGAGGGCTTCATGAAATCCTCCCTCCTATTGAAGTTGTTGTAGGCTCAATATCAAATGCTGACCCAGCTTGTCCAGATGAGTGGGAAGATGACTTAGATGAGCGCATAGAGTACAATGCTGACAATACCATTAAGACTGAGATTGTCAAATCTCCTTTCATTCAGATTCCACTAGGAGTTACAGAAGACAGACTCATTGGGTCTGTTGATGTTGAGGAGTCTGTGAAAAGGGGGACAACTGTTTTCCAACCTGGTCTTTTGGCTGAAGCCCATAGAGGAGTGTTGTATGTTGATGAAATAAATCTCTTAGATGAGGGAATTAGTAATTTGCTTCTCAATGTATTGACGGATGGTGTTAATATAGTTGAAAGAGAAGGAATCAGCTTTAGGCACCCGTGCAAACCACTTTTAATTGCAACCTATAACCCTGAAGAAGGTGCTGTTCGAGAGCACTTGCTAGACCGTGTTGCCATTAATTTAAGTGCAGACCTACCTATGAGTTTTGAAGATCGTGTCGCAGCAGTTGGAATTGCCACACAGTTTCAGGAACGCTGTAATGAGGTTTTTAGAATGGTAAATGAAGAGACAGAAACAGCAAAGACGCAGATTATATTGGCTAGAGAATATTTGAAAGATGTCAAGATAAGTAGAGAGCAATTGAAGTATCTGGTTTTGGAAGCTGTCCGAGGTGGTGTCCAGGGACACCGCGCCGAATTGTATGCAGCTCGTGTGGCGAAGTGTTTAGCTGCAATTGAAGGACGAGAAAAAGTCACAATCGATGACCTCAGAAAGGCCGTTGAGCTGGTCATTCTTCCTCGTTCATCACTAGATGAGACTCCACCTGAACAACAAAACCAACCACCACCTCCTCCACCTCCTCCACAAAATAGCGAATCTGGAGAAGAAGAAAATGAAGAAGAACAAGAAGAAGAAGAAGAGGATGAAAGCAATGAAGAAAATGAAAATGAGCAGCAACAGGACCAAATACCTGAAGAGTTTATATTTGACGCTGAGGGCGGTCTGGTGGATGAGAAACTCCTCTTCTTTGCTCAACAAGCCCAGAAACGTCGGGGGAAAGCTGGCAGGGCGAAGAATGTCATATTCTCAGAAGATAGAGGACGCTACATAAAGCCAATGCTTCCAAAGGGTCCAGTAAAAAGATTAGCTGTGGATGCAACCCTTAGAGCAGCTGCACCATACCAGAAATTGCGCAGAGAGAAGGATATCTCAGGAACTAGGAAAGTCTTTGTTGAGAAGACAGATATGAGGGCCAAAAGAATGGCAAGGAAAGCTGGAGCCCTGGTTATCTTTGTGGTTGATGCAAGTGGCAGTATGGCATTGAATCGTATGCAAAACGCCAAAGGTGCTGCACTCAAACTACTGGCAGAGAGCTATACTAGCAGGGATCAGGTTTCGATTATTCCTTTCCGAGGGGATGCTGCGGAAGTGCTCTTGCCCCCTTCTAGATCAATAGCAATGGCAAGGAATCGTCTTGAGAGACTTCCTTGTGGTGGTGGTTCTCCTCTTGCCCATGGTTTAACAACGGCTGTAAGAGTAGGACTTAACGCAGAGAAGAGTGGTGATGTCGGGCGCATAATGATTGTTGCGATAACCGATGGTCGAGCCAACATTACACTGAAAAGATCAACTGATCCGGAGTCTATTGCCCCAGATGCTCCTAGACCCACGTCCAAAGAATTGAAGGATGAGATTCTGGAAGTTGCTGGGAAGATATACAAGGCAGGGATGTCTCTTCTAGTGATTGATACCGAGAACAAGTTTGTTTCAACTGGTTTTGCAAAGGAGATCGCAAGAGTTGCTCAAGGAAAATATTATTACTTGCCAAATGCTTCGGATGCTGTAATCTCGGCCACCACTAGGGATGCACTATCTGATCTGAAGAATTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009532 GO:0009534 GO:0009536 GO:0009570 GO:0009579 GO:0010007 GO:0031976 GO:0031984 GO:0032991 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:1902494

KEGG Pathways

Metabolic & Signaling

ko00860 ko01100 ko01110 map00860 map01100 map01110

Pfam Domains

Protein Families

Mg_chelatase VWA_2

Protein Analysis

760

Amino Acids

83.28

Weight (kDa)

5.26

Isoelectric Point (pI)

43.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Mg_chelatase	PF01078	202 - 270	1.6e-06	Magnesium chelatase, subunit ChlI
AAA_lid_2	PF17863	349 - 408	6.8e-14	AAA lid domain
VWA_2	PF13519	559 - 664	2.1e-12	von Willebrand factor type A domain
VWA	PF00092	559 - 677	2.9e-06	von Willebrand factor type A domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005823)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G08520
fragaria_vesca	FvH4_3g28280 FvH4_3g28280 FvH4_3g28280 FvH4_3g28280 FvH4_3g28280 FvH4_3g28280 FvH4_3g28280 FvH4_3g28280 FvH4_3g28280 FvH4_3g28280 FvH4_3g28280
malus_domestica	MD03G1170000.v1.1 MD11G1187400.v1.1
prunus_persica	Prupe.4G257100_v2.0.a1 Prupe.4G257100_v2.0.a1
pyrus_communis	pycom11g16410 pycom11g16420
rosa_chinensis	RchiOBHm_Chr5g0051161
rosa_laevigata	RLG00000034758
rosa_multiflora	Rmu_sc0000446.1_g000012
rosa_roxburghii	Rroxscaffold_1G00029290
rosa_samantha	Rh5AG338500 Rh5BG348400 Rh5CG375500 Rh5DG361300
rosa_wichuraiana	Rw0G023660 Rw5G031970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1583
Acc36I	ACCTGC	1 cut(s)	50
AccB1I	GGYRCC	1 cut(s)	762
AccII	CGCG	5 cut(s)	20, 155, 305, 373, 1080
AccIII	TCCGGA	1 cut(s)	2026
AciI	CCGC	7 cut(s)	133, 153, 258, 305, 1078, 1389, 1814
AclWI	GGATC	2 cut(s)	1785, 2018
AcoI	YGGCCR	4 cut(s)	235, 255, 268, 2241
AcsI	RAATTY	1 cut(s)	2275
AcuI	CTGAAG	3 cut(s)	657, 822, 1383
AfaI	GTAC	2 cut(s)	144, 485
AfiI	CCNNNNNNNGG	5 cut(s)	806, 1162, 1802, 1883, 2250
AjiI	CACGTC	1 cut(s)	2061
AjnI	CCWGG	4 cut(s)	270, 622, 1068, 1671
Alw21I	GWGCWC	2 cut(s)	825, 1824
Alw26I	GTCTC	5 cut(s)	209, 950, 1199, 1864, 2127
AlwI	GGATC	2 cut(s)	1785, 2018
Aor13HI	TCCGGA	1 cut(s)	2026
AoxI	GGCC	6 cut(s)	235, 255, 268, 1164, 1641, 2241
ApeKI	GCWGC	9 cut(s)	57, 890, 1094, 1118, 1342, 1559, 1562, 1739, 1811
ApoI	RAATTY	1 cut(s)	2275
AseI	ATTAAT	1 cut(s)	846
AspLEI	GCGC	6 cut(s)	305, 373, 477, 1082, 1584, 1965
AspS9I	GGNCC	3 cut(s)	1351, 1520, 1641
AsuHPI	GGTGA	1 cut(s)	1964
AvaII	GGWCC	2 cut(s)	1351, 1520
BalI	TGGCCA	1 cut(s)	270
BanI	GGYRCC	1 cut(s)	762
BanII	GRGCYC	1 cut(s)	1672
BauI	CACGAG	2 cut(s)	127, 1098
BbsI	GAAGAC	2 cut(s)	564, 1634
Bbv12I	GWGCWC	2 cut(s)	825, 1824
BbvCI	CCTCAGC	1 cut(s)	1383
BbvI	GCAGC	9 cut(s)	44, 902, 1105, 1106, 1354, 1549, 1571, 1726, 1798
BccI	CCATC	2 cut(s)	716, 1982
BceAI	ACGGC	2 cut(s)	1151, 1932
BcgI	CGANNNNNNTGC	2 cut(s)	1073, 1107
BciT130I	CCWGG	4 cut(s)	272, 624, 1070, 1673
BciVI	GTATCC	2 cut(s)	202, 214
BcoDI	GTCTC	5 cut(s)	209, 950, 1199, 1864, 2127
BfmI	CTRYAG	1 cut(s)	43
BfuAI	ACCTGC	1 cut(s)	50
BfuI	GTATCC	2 cut(s)	202, 214
BglI	GCCNNNNNGGC	1 cut(s)	276
Bme1390I	CCNGG	4 cut(s)	272, 624, 1070, 1673
Bme18I	GGWCC	2 cut(s)	1351, 1520
BmgBI	CACGTC	1 cut(s)	2061
BmgT120I	GGNCC	3 cut(s)	1351, 1520, 1641
BmiI	GGNNCC	3 cut(s)	764, 1521, 1669
BmrFI	CCNGG	4 cut(s)	272, 624, 1070, 1673
BmsI	GCATC	6 cut(s)	1534, 1681, 1798, 2035, 2218, 2245
BoxI	GACNNNNGTC	2 cut(s)	14, 2059
BpiI	GAAGAC	2 cut(s)	564, 1634
BpmI	CTGGAG	3 cut(s)	15, 1290, 1686
Bpu10I	CCTNAGC	2 cut(s)	84, 1383
BpuEI	CTTGAG	2 cut(s)	1886, 2178
Bsa29I	ATCGAT	1 cut(s)	1149
BsaAI	YACGTR	1 cut(s)	146
BsaJI	CCNNGG	5 cut(s)	1057, 1069, 1671, 1801, 1903
BsaWI	WCCGGW	2 cut(s)	13, 2026
BsaXI	ACNNNNNCTCC	2 cut(s)	1659, 1689
Bsc4I	CCNNNNNNNGG	5 cut(s)	806, 1162, 1802, 1883, 2250
Bse1I	ACTGG	4 cut(s)	32, 1523, 1758, 2167
Bse3DI	GCAATG	2 cut(s)	1327, 1854
BseAI	TCCGGA	1 cut(s)	2026
BseBI	CCWGG	4 cut(s)	272, 624, 1070, 1673
BseCI	ATCGAT	1 cut(s)	1149
BseDI	CCNNGG	5 cut(s)	1057, 1069, 1671, 1801, 1903
BseGI	GGATG	9 cut(s)	727, 1318, 1405, 1549, 1813, 2083, 2124, 2233, 2260
BseLI	CCNNNNNNNGG	5 cut(s)	806, 1162, 1802, 1883, 2250
BseMI	GCAATG	2 cut(s)	1327, 1854
BseMII	CTCAG	5 cut(s)	501, 1171, 1374, 1491, 1614
BseNI	ACTGG	4 cut(s)	32, 1523, 1758, 2167
BseRI	GAGGAG	7 cut(s)	56, 602, 663, 1230, 1239, 1400, 1884
BseXI	GCAGC	9 cut(s)	44, 902, 1105, 1106, 1354, 1549, 1571, 1726, 1798
BseYI	CCCAGC	2 cut(s)	436, 2096
BsgI	GTGCAG	3 cut(s)	876, 1548, 1725
Bsh1236I	CGCG	5 cut(s)	20, 155, 305, 373, 1080
Bsh1285I	CGRYCG	1 cut(s)	18
BshFI	GGCC	6 cut(s)	237, 257, 270, 1166, 1643, 2243
BshNI	GGYRCC	1 cut(s)	762
BshVI	ATCGAT	1 cut(s)	1149
BsiEI	CGRYCG	1 cut(s)	18
BsiHKAI	GWGCWC	2 cut(s)	825, 1824
BsiSI	CCGG	2 cut(s)	14, 2027
BslFI	GGGAC	3 cut(s)	170, 619, 1086
BslI	CCNNNNNNNGG	5 cut(s)	806, 1162, 1802, 1883, 2250
BsmAI	GTCTC	5 cut(s)	209, 950, 1199, 1864, 2127
BsmBI	CGTCTC	1 cut(s)	209
BsmFI	GGGAC	3 cut(s)	170, 619, 1086
BsmI	GAATGC	1 cut(s)	163
BsnI	GGCC	6 cut(s)	237, 257, 270, 1166, 1643, 2243
Bsp1286I	GDGCHC	3 cut(s)	825, 1672, 1824
Bsp13I	TCCGGA	1 cut(s)	2026
Bsp143I	GATC	9 cut(s)	73, 310, 880, 1777, 1838, 2015, 2023, 2178, 2266
Bsp19I	CCATGG	1 cut(s)	1903
BspACI	CCGC	7 cut(s)	133, 153, 258, 305, 1078, 1389, 1814
BspANI	GGCC	6 cut(s)	237, 257, 270, 1166, 1643, 2243
BspCNI	CTCAG	5 cut(s)	502, 1170, 1375, 1490, 1613
BspDI	ATCGAT	1 cut(s)	1149
BspEI	TCCGGA	1 cut(s)	2026
BspFNI	CGCG	5 cut(s)	20, 155, 305, 373, 1080
BspHI	TCATGA	1 cut(s)	382
BspLI	GGNNCC	3 cut(s)	764, 1521, 1669
BspMAI	CTGCAG	1 cut(s)	47
BspMI	ACCTGC	1 cut(s)	50
BspPI	GGATC	2 cut(s)	1785, 2018
BspT107I	GGYRCC	1 cut(s)	762
BsrDI	GCAATG	2 cut(s)	1327, 1854
BsrI	ACTGG	4 cut(s)	32, 1523, 1758, 2167
BssECI	CCNNGG	5 cut(s)	1057, 1069, 1671, 1801, 1903
BssMI	GATC	9 cut(s)	73, 310, 880, 1777, 1838, 2015, 2023, 2178, 2266
BssSI	CACGAG	2 cut(s)	127, 1098
BssT1I	CCWWGG	1 cut(s)	1903
Bst2BI	CACGAG	2 cut(s)	127, 1098
Bst2UI	CCWGG	4 cut(s)	272, 624, 1070, 1673
Bst4CI	ACNGT	8 cut(s)	142, 166, 182, 243, 364, 613, 836, 912
Bst6I	CTCTTC	6 cut(s)	948, 1302, 1359, 1418, 1940, 2130
BstAPI	GCANNNNNTGC	1 cut(s)	2189
BstBAI	YACGTR	1 cut(s)	146
BstC8I	GCNNGC	1 cut(s)	1453
BstDEI	CTNAG	9 cut(s)	84, 466, 510, 679, 1157, 1383, 1477, 1553, 1600
BstDSI	CCRYGG	1 cut(s)	1903
BstENI	CCTNNNNNAGG	3 cut(s)	804, 1160, 1800
BstF5I	GGATG	9 cut(s)	727, 1318, 1405, 1549, 1813, 2083, 2124, 2233, 2260
BstFNI	CGCG	5 cut(s)	20, 155, 305, 373, 1080
BstHHI	GCGC	6 cut(s)	305, 373, 477, 1082, 1584, 1965
BstKTI	GATC	9 cut(s)	76, 313, 883, 1780, 1841, 2018, 2026, 2181, 2269
BstMAI	GTCTC	5 cut(s)	209, 950, 1199, 1864, 2127
BstMBI	GATC	9 cut(s)	73, 310, 880, 1777, 1838, 2015, 2023, 2178, 2266
BstMCI	CGRYCG	1 cut(s)	18
BstNI	CCWGG	4 cut(s)	272, 624, 1070, 1673
BstPAI	GACNNNNGTC	2 cut(s)	14, 2059
BstSCI	CCNGG	4 cut(s)	270, 622, 1068, 1671
BstSFI	CTRYAG	1 cut(s)	43
BstUI	CGCG	5 cut(s)	20, 155, 305, 373, 1080
BstV1I	GCAGC	9 cut(s)	44, 902, 1105, 1106, 1354, 1549, 1571, 1726, 1798
BstV2I	GAAGAC	2 cut(s)	564, 1634
BstXI	CCANNNNNNTGG	2 cut(s)	453, 1651
Bsu15I	ATCGAT	1 cut(s)	1149
BsuI	GTATCC	2 cut(s)	202, 214
BsuRI	GGCC	6 cut(s)	237, 257, 270, 1166, 1643, 2243
BsuTUI	ATCGAT	1 cut(s)	1149
BtgI	CCRYGG	1 cut(s)	1903
BtgZI	GCGATG	1 cut(s)	20
BtrI	CACGTC	1 cut(s)	2061
BtsCI	GGATG	9 cut(s)	727, 1318, 1405, 1549, 1813, 2083, 2124, 2233, 2260
BtsI	GCAGTG	1 cut(s)	351
BtsIMutI	CAGTG	3 cut(s)	138, 351, 2006
BveI	ACCTGC	1 cut(s)	50
Cac8I	GCNNGC	1 cut(s)	1453
CciI	TCATGA	1 cut(s)	382
CfoI	GCGC	6 cut(s)	305, 373, 477, 1082, 1584, 1965
Cfr13I	GGNCC	3 cut(s)	1351, 1520, 1641
ClaI	ATCGAT	1 cut(s)	1149
CseI	GACGC	4 cut(s)	9, 982, 1388, 1500
CsiI	ACCWGGT	1 cut(s)	622
Csp6I	GTAC	2 cut(s)	143, 484
CviAII	CATG	3 cut(s)	367, 383, 1904
CviQI	GTAC	2 cut(s)	143, 484
DdeI	CTNAG	9 cut(s)	84, 466, 510, 679, 1157, 1383, 1477, 1553, 1600
DpnI	GATC	9 cut(s)	75, 312, 882, 1779, 1840, 2017, 2025, 2180, 2268
DpnII	GATC	9 cut(s)	73, 310, 880, 1777, 1838, 2015, 2023, 2178, 2266
EaeI	YGGCCR	4 cut(s)	235, 255, 268, 2241
Eam1104I	CTCTTC	6 cut(s)	948, 1302, 1359, 1418, 1940, 2130
EarI	CTCTTC	6 cut(s)	948, 1302, 1359, 1418, 1940, 2130
Eco130I	CCWWGG	1 cut(s)	1903
Eco24I	GRGCYC	1 cut(s)	1672
Eco32I	GATATC	1 cut(s)	1597
Eco47I	GGWCC	2 cut(s)	1351, 1520
Eco57I	CTGAAG	3 cut(s)	657, 822, 1383
EcoNI	CCTNNNNNAGG	3 cut(s)	804, 1160, 1800
EcoRI	GAATTC	1 cut(s)	2275
EcoRII	CCWGG	4 cut(s)	270, 622, 1068, 1671
EcoRV	GATATC	1 cut(s)	1597
EcoT14I	CCWWGG	1 cut(s)	1903
EcoT38I	GRGCYC	1 cut(s)	1672
ErhI	CCWWGG	1 cut(s)	1903
Esp3I	CGTCTC	1 cut(s)	209
FaeI	CATG	3 cut(s)	370, 386, 1907
FaqI	GGGAC	3 cut(s)	170, 619, 1086
FatI	CATG	3 cut(s)	366, 382, 1903
FokI	GGATG	9 cut(s)	734, 1325, 1412, 1556, 1820, 2090, 2131, 2240, 2267
FriOI	GRGCYC	1 cut(s)	1672
FspI	TGCGCA	1 cut(s)	1583
GlaI	GCGC	6 cut(s)	304, 372, 476, 1081, 1583, 1964
GsaI	CCCAGC	2 cut(s)	440, 2100
GsuI	CTGGAG	3 cut(s)	15, 1290, 1686
HaeIII	GGCC	6 cut(s)	237, 257, 270, 1166, 1643, 2243
HapII	CCGG	2 cut(s)	14, 2027
HgaI	GACGC	4 cut(s)	9, 982, 1388, 1500
HhaI	GCGC	6 cut(s)	305, 373, 477, 1082, 1584, 1965
Hin1II	CATG	3 cut(s)	370, 386, 1907
Hin6I	GCGC	6 cut(s)	303, 371, 475, 1080, 1582, 1963
HinP1I	GCGC	6 cut(s)	303, 371, 475, 1080, 1582, 1963
HpaII	CCGG	2 cut(s)	14, 2027
HphI	GGTGA	1 cut(s)	1964
Hpy99I	CGWCG	2 cut(s)	170, 1443
HpyAV	CCTTC	7 cut(s)	269, 740, 800, 1121, 1585, 1842, 2068
HpyCH4III	ACNGT	8 cut(s)	142, 166, 182, 243, 364, 613, 836, 912
HpyCH4IV	ACGT	3 cut(s)	145, 1438, 2060
HpyF3I	CTNAG	9 cut(s)	84, 466, 510, 679, 1157, 1383, 1477, 1553, 1600
HpySE526I	ACGT	3 cut(s)	145, 1438, 2060
Hsp92II	CATG	3 cut(s)	370, 386, 1907
HspAI	GCGC	6 cut(s)	303, 371, 475, 1080, 1582, 1963
Kpn2I	TCCGGA	1 cut(s)	2026
Kzo9I	GATC	9 cut(s)	73, 310, 880, 1777, 1838, 2015, 2023, 2178, 2266
LmnI	GCTCC	2 cut(s)	1667, 2053
Lsp1109I	GCAGC	9 cut(s)	44, 902, 1105, 1106, 1354, 1549, 1571, 1726, 1798
LweI	GCATC	6 cut(s)	1534, 1681, 1798, 2035, 2218, 2245
MabI	ACCWGGT	1 cut(s)	622
MaeII	ACGT	3 cut(s)	145, 1438, 2060
MaeIII	GTNAC	2 cut(s)	550, 1141
MalI	GATC	9 cut(s)	75, 312, 882, 1779, 1840, 2017, 2025, 2180, 2268
MboI	GATC	9 cut(s)	73, 310, 880, 1777, 1838, 2015, 2023, 2178, 2266
MfeI	CAATTG	2 cut(s)	1028, 1122
MhlI	GDGCHC	3 cut(s)	825, 1672, 1824
MlsI	TGGCCA	1 cut(s)	270
MluNI	TGGCCA	1 cut(s)	270
MlyI	GAGTC	6 cut(s)	4, 42, 558, 599, 1201, 2039
MmeI	TCCRAC	2 cut(s)	643, 877
Mox20I	TGGCCA	1 cut(s)	270
MroI	TCCGGA	1 cut(s)	2026
MscI	TGGCCA	1 cut(s)	270
MseI	TTAA	7 cut(s)	504, 729, 782, 846, 851, 1910, 1935
Msp20I	TGGCCA	1 cut(s)	270
MspA1I	CMGCKG	1 cut(s)	1562
MspI	CCGG	2 cut(s)	14, 2027
MspR9I	CCNGG	4 cut(s)	272, 624, 1070, 1673
MunI	CAATTG	2 cut(s)	1028, 1122
Mva1269I	GAATGC	1 cut(s)	163
MvaI	CCWGG	4 cut(s)	272, 624, 1070, 1673
MvnI	CGCG	5 cut(s)	20, 155, 305, 373, 1080
NcoI	CCATGG	1 cut(s)	1903
NdeII	GATC	9 cut(s)	73, 310, 880, 1777, 1838, 2015, 2023, 2178, 2266
NlaIII	CATG	3 cut(s)	370, 386, 1907
NlaIV	GGNNCC	3 cut(s)	764, 1521, 1669
NmeAIII	GCCGAG	1 cut(s)	2219
NmuCI	GTSAC	1 cut(s)	1141
NsbI	TGCGCA	1 cut(s)	1583
PagI	TCATGA	1 cut(s)	382
PctI	GAATGC	1 cut(s)	163
PfeI	GAWTC	7 cut(s)	170, 538, 750, 1265, 1714, 1858, 2083
PleI	GAGTC	6 cut(s)	4, 42, 558, 598, 1201, 2038
PpsI	GAGTC	6 cut(s)	4, 42, 558, 598, 1201, 2038
Ppu21I	YACGTR	1 cut(s)	146
PshAI	GACNNNNGTC	2 cut(s)	14, 2059
PshBI	ATTAAT	1 cut(s)	846
Psp6I	CCWGG	4 cut(s)	270, 622, 1068, 1671
PspFI	CCCAGC	2 cut(s)	436, 2096
PspGI	CCWGG	4 cut(s)	270, 622, 1068, 1671
PspN4I	GGNNCC	3 cut(s)	764, 1521, 1669
PspPI	GGNCC	3 cut(s)	1351, 1520, 1641
PstI	CTGCAG	1 cut(s)	47
PvuII	CAGCTG	1 cut(s)	1562
RsaI	GTAC	2 cut(s)	144, 485
RsaNI	GTAC	2 cut(s)	143, 484
SaqAI	TTAA	7 cut(s)	504, 729, 782, 846, 851, 1910, 1935
Sau3AI	GATC	9 cut(s)	73, 310, 880, 1777, 1838, 2015, 2023, 2178, 2266
Sau96I	GGNCC	3 cut(s)	1351, 1520, 1641
SchI	GAGTC	6 cut(s)	4, 42, 558, 599, 1201, 2039
ScrFI	CCNGG	4 cut(s)	272, 624, 1070, 1673
SduI	GDGCHC	3 cut(s)	825, 1672, 1824
SexAI	ACCWGGT	1 cut(s)	622
SfaNI	GCATC	6 cut(s)	1534, 1681, 1798, 2035, 2218, 2245
SfcI	CTRYAG	1 cut(s)	43
SinI	GGWCC	2 cut(s)	1351, 1520
SmlI	CTYRAG	2 cut(s)	1865, 2193
SmoI	CTYRAG	2 cut(s)	1865, 2193
SsiI	CCGC	7 cut(s)	133, 153, 258, 305, 1078, 1389, 1814
SspI	AATATT	2 cut(s)	998, 2204
StyD4I	CCNGG	4 cut(s)	270, 622, 1068, 1671
StyI	CCWWGG	1 cut(s)	1903
TaaI	ACNGT	8 cut(s)	142, 166, 182, 243, 364, 613, 836, 912
TaiI	ACGT	3 cut(s)	148, 1441, 2063
TaqI	TCGA	5 cut(s)	168, 817, 1149, 1787, 1993
TatI	WGTACW	1 cut(s)	483
TauI	GCSGC	2 cut(s)	135, 260
TfiI	GAWTC	7 cut(s)	170, 538, 750, 1265, 1714, 1858, 2083
Tru1I	TTAA	7 cut(s)	504, 729, 782, 846, 851, 1910, 1935
Tru9I	TTAA	7 cut(s)	504, 729, 782, 846, 851, 1910, 1935
TscAI	CASTG	3 cut(s)	145, 358, 2013
TseFI	GTSAC	1 cut(s)	1141
TseI	GCWGC	9 cut(s)	57, 890, 1094, 1118, 1342, 1559, 1562, 1739, 1811
Tsp45I	GTSAC	1 cut(s)	1141
TspGWI	ACGGA	4 cut(s)	221, 227, 233, 734
TspRI	CASTG	3 cut(s)	145, 358, 2013
VpaK11BI	GGWCC	2 cut(s)	1351, 1520
VspI	ATTAAT	1 cut(s)	846
XagI	CCTNNNNNAGG	3 cut(s)	804, 1160, 1800
XapI	RAATTY	1 cut(s)	2275
XbaI	TCTAGA	1 cut(s)	1835

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.