Rh5CG375500

Involved in chlorophyll biosynthesis. Catalyzes the insertion of magnesium ion into protoporphyrin IX to yield Mg- protoporphyrin IX

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0005823
Physical Location & Seq
Reverse (-)
48562327 .. 48571137
8811 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG375500.1

Sequence Viewer

Length: 2295 bp
ATGGCTTTCTCACACACCTCGACCTCGTCGTCGCCATGCACATCATTTCTCTCGCACCTACAATCTTCTTCTTCTTCTTCGTCTTCGCTACTCAAATTACAGCACCCCCTCCTCCTCCTCCTCTCCCAACGTCGCGCTCGCATTCGAATTCGAATTCGCGTCACTGCTTCTGCAGCAGCTGCCGTTGTTGAGTCTTCAAACGGCGCCGTAGCGGCGAAACAGGACAGTTCCTACGGAAGGCAGTTCTTTCCCTTGGCTGCCGTTGTTGGCCAAGATGCTATTAAAACTGCTCTGTTGCTTGGGGCTATAGACCGTGAAATTGGAGGAATTGCTATCTCCGGAAGGAGGGGAACAGCCAAAACAGTAATGGCCCGTGGTTTGCATGCAATTTTACCACCCATTGAAGTAGTCGTTGGTTCAATAGCAAATGCTGATCCAGCCTGTCCCGAAGAGTGGGAAGATGTTTTACCTGAACGCATGGAATTTGATTCTGCTGGTAATGTCAAGACTCAGATTGTTAGGTCTCCTTTTGTTCAGATTCCACTTGGAGTCACAGAGGACAGACTCATTGGATCTGTTGATGTTGAGGAGTCCGTGAGAACAGGAACCACTGTTTTCCAACCTGGCCTTCTTGCTGAAGCTCATAGAGGTGTATTATATGTTGATGAAATTAATCTTTTGGATGAGGGGATCAGTAATTTGCTTCTCAACGTATTAACTGAAGGAGTTAATATTGTTGAGAGAGAGGGAATGAGCTTTAGACACCCATGCAAGCCACTCTTGATTGCTACGTATAATCCAGAAGAGGGTGCTGTGCGTGAACACTTACTAGACCGTATTGCAATCAACTTGAGTGCAGATCTTCCGATGAGTTTTGATGATCGTGTTGCAGCTGTGGGCATTGCGACAAAATTTCAGGAATATAGTACTGAAGTCTTTAAAATGGTTCAAGAAGAAACAGAATTTGCAAAAACTCAGATCATTTTGGCAAGAGAGTATCTAAAGGACGTAGTTATAAGCAGAGAACAACTCAAGTATCTGGTTTTGGAAGCCCTGCGAGGTGGTTGCCAGGGACATAGAGCTGAGCTATATGCTTCCCGTGTTGCAAAATGCTTAGCTGCTCTAGATGGACGAGAAAAAGTTTATGCTGATGACCTGAAAAAAGCTGTAGAACTGGTCATACTTCCTCGCTCAATCGTTAATGAGAACCCACCAGAACAGCAAAATCAACCGCCTCCACCTCCACCACCTCCTCAAAATCAAGAATCTGGGGAAGAGCAAAATGAGGAGCAAGAAGAAAAAGAGGATGAAAATGATGAAGAGAAGGAAGAGAATGAAGATAATGAACAAGAGCAGGAACAGCTACCTGAAGAGTTTGTCTTTGATGCAGAAGGGGGTTTGGTGGATGAGAAGCTTCTTTTTTTCGCACAACAAGCGCAGAGACGCAAAGGGAAGGCTGGGAGAGCAAAGAATGTTATCTTTTCAGAGGATAGAGGTCGATACATTAAGCCAATGCTTCCCAAGGGACCTGTAAAGAGATTAGCTGTTGATGCAACTCTTAGAGTTGCTGCACCATATCAAAAATTGCGTAGGGAGAAGAACACTCAAGTCGGCAGGAAAGTTTTTGTTGAAAAAACTGACATGAGAGCAAAGAGAATGGCACGGAAAGCAGGAGCACTGGTTATATTTGTTGTTGATGCAAGTGGAAGCATGGCACTGAACCGAATGCAGAATGCAAAAGGTGCAGCGCTCAAGTTACTAGCCGAAAGTTACACGAGCAGAGATCAGGTTTCTATTATTCCTTTCCGTGGAGATTCTGCTGAAGTTCTTCTGCCTCCTTCTAGATCCATTGCAATGGCAAGAAATCGTCTTGAGAGACTTCCCTGTGGTGGAGGTTCTCCCCTTGCTCATGGTCTGACCACGGCTGTAAGGGTTGGGCTAAATGCAGAGAAGAGTGGTGATGTTGGACGTATAATGATTGTTGCAATAACTGATGGTAGAGCCAATATATCACTAAAAAGGTCCAATGACCCTGAAGCTGCTGCTGCTGCTACTGATGTCCCAAGACCTTCGGTAAAGGAGTTGAAGGATGAGATTCTTGAGGTTGCTGGTAAAATATACAAAGCAGGGATGTCTCTTCTCGTCATCGACACTGAAAATAAATTTGTTTCAACTGGTTTTGCCAAGGAGATTGCAAGAGTTGCTCAAGGAAAATACTATTACTTGCCAAATGCCTCAGATGCTGTAATCTCAGCTGCCACAAAGGACGCTTTGTCAACATTGAAAAACACGTGA

Protein Analysis

764

Amino Acids

83.46

Weight (kDa)

5.96

Isoelectric Point (pI)

46.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mg_chelatase PF01078 202 - 269 9.8e-07 Magnesium chelatase, subunit ChlI
AAA_lid_2 PF17863 349 - 409 1.4e-13 AAA lid domain
VWA_2 PF13519 561 - 666 1.6e-12 von Willebrand factor type A domain
VWA PF00092 561 - 682 4.1e-06 von Willebrand factor type A domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1016
AasI GACNNNNNNGTC 1 cut(s) 28
AccB1I GGYRCC 1 cut(s) 203
AccII CGCG 2 cut(s) 135, 159
AccIII TCCGGA 1 cut(s) 338
AciI CCGC 2 cut(s) 212, 1232
AclWI GGATC 4 cut(s) 428, 580, 698, 1839
AcoI YGGCCR 1 cut(s) 268
AcsI RAATTY 6 cut(s) 147, 153, 482, 911, 962, 2162
AcuI CTGAAG 6 cut(s) 657, 741, 951, 1389, 1842, 2055
AcvI CACGTG 1 cut(s) 2292
AcyI GRCGYC 1 cut(s) 204
AfaI GTAC 1 cut(s) 928
AfeI AGCGCT 1 cut(s) 1749
AfiI CCNNNNNNNGG 6 cut(s) 237, 345, 453, 806, 1808, 1889
AflIII ACRYGT 1 cut(s) 2289
AgsI TTSAA 8 cut(s) 198, 404, 420, 950, 1631, 2086, 2172, 2284
AhdI GACNNNNNGTC 1 cut(s) 2272
AjnI CCWGG 2 cut(s) 622, 1068
AjuI GAANNNNNNNTTGG 4 cut(s) 396, 428, 612, 644
Alw21I GWGCWC 1 cut(s) 1678
Alw26I GTCTC 4 cut(s) 528, 1435, 1870, 2139
AlwI GGATC 4 cut(s) 428, 580, 698, 1839
AlwNI CAGNNNCTG 2 cut(s) 179, 2243
Aor13HI TCCGGA 1 cut(s) 338
Aor51HI AGCGCT 1 cut(s) 1749
AoxI GGCC 3 cut(s) 268, 369, 625
ApoI RAATTY 6 cut(s) 147, 153, 482, 911, 962, 2162
AseI ATTAAT 1 cut(s) 672
Asp700I GAANNNNTTC 1 cut(s) 1827
AspLEI GCGC 4 cut(s) 137, 206, 1438, 1750
AspS9I GGNCC 3 cut(s) 370, 1526, 2022
AsuHPI GGTGA 1 cut(s) 1970
AsuII TTCGAA 2 cut(s) 145, 151
AvaII GGWCC 2 cut(s) 1526, 2022
BaeI ACNNNNGTAYC 2 cut(s) 1019, 1052
BalI TGGCCA 1 cut(s) 270
BanI GGYRCC 1 cut(s) 203
BarI GAAGNNNNNNTAC 2 cut(s) 450, 482
BauI CACGAG 1 cut(s) 1774
BbrPI CACGTG 1 cut(s) 2292
BbsI GAAGAC 2 cut(s) 75, 186
Bbv12I GWGCWC 1 cut(s) 1678
BccI CCATC 2 cut(s) 1121, 1988
BceAI ACGGC 5 cut(s) 167, 191, 217, 245, 1938
BcgI CGANNNNNNTGC 2 cut(s) 1047, 1081
BciT130I CCWGG 2 cut(s) 624, 1070
BcoDI GTCTC 4 cut(s) 528, 1435, 1870, 2139
BfaI CTAG 4 cut(s) 830, 1124, 1760, 1842
BfmI CTRYAG 3 cut(s) 171, 306, 1167
BfoI RGCGCY 2 cut(s) 207, 1751
BglI GCCNNNNNGGC 1 cut(s) 212
BglII AGATCT 1 cut(s) 859
BlpI GCTNAGC 2 cut(s) 1083, 1114
BmcAI AGTACT 1 cut(s) 928
Bme1390I CCNGG 2 cut(s) 624, 1070
Bme18I GGWCC 2 cut(s) 1526, 2022
BmeRI GACNNNNNGTC 1 cut(s) 2272
BmgT120I GGNCC 3 cut(s) 370, 1526, 2022
BmiI GGNNCC 3 cut(s) 205, 607, 1527
BmrFI CCNGG 2 cut(s) 624, 1070
BmsI GCATC 5 cut(s) 265, 1375, 1540, 1687, 2230
BpiI GAAGAC 2 cut(s) 75, 186
BplI GAGNNNNNCTC 2 cut(s) 1014, 1046
Bpu1102I GCTNAGC 2 cut(s) 1083, 1114
Bpu14I TTCGAA 2 cut(s) 145, 151
BpuEI CTTGAG 7 cut(s) 871, 1016, 1590, 1736, 1892, 2120, 2190
BsaAI YACGTR 2 cut(s) 792, 2292
BsaHI GRCGYC 1 cut(s) 204
BsaI GGTCTC 1 cut(s) 528
BsaJI CCNNGG 7 cut(s) 252, 373, 1069, 1521, 1807, 1920, 2184
BsaWI WCCGGW 1 cut(s) 338
BsaXI ACNNNNNCTCC 2 cut(s) 1665, 1695
Bsc4I CCNNNNNNNGG 6 cut(s) 237, 345, 453, 806, 1808, 1889
Bse1I ACTGG 3 cut(s) 1179, 1683, 2179
Bse3DI GCAATG 3 cut(s) 900, 1848, 1860
BseAI TCCGGA 1 cut(s) 338
BseBI CCWGG 2 cut(s) 624, 1070
BseDI CCNNGG 7 cut(s) 252, 373, 1069, 1521, 1807, 1920, 2184
BseGI GGATG 5 cut(s) 688, 1312, 1411, 2095, 2136
BseLI CCNNNNNNNGG 6 cut(s) 237, 345, 453, 806, 1808, 1889
BseMI GCAATG 3 cut(s) 900, 1848, 1860
BseMII CTCAG 5 cut(s) 524, 989, 1074, 2250, 2265
BseNI ACTGG 3 cut(s) 1179, 1683, 2179
BseRI GAGGAG 7 cut(s) 101, 104, 107, 110, 602, 1242, 1301
BseYI CCCAGC 1 cut(s) 1457
BsgI GTGCAG 3 cut(s) 876, 1554, 1764
Bsh1236I CGCG 2 cut(s) 135, 159
BshFI GGCC 3 cut(s) 270, 371, 627
BshNI GGYRCC 1 cut(s) 203
BsiHKAI GWGCWC 1 cut(s) 1678
BsiSI CCGG 1 cut(s) 339
BslFI GGGAC 4 cut(s) 429, 1086, 1539, 2045
BslI CCNNNNNNNGG 6 cut(s) 237, 345, 453, 806, 1808, 1889
BsmAI GTCTC 4 cut(s) 528, 1435, 1870, 2139
BsmBI CGTCTC 1 cut(s) 1435
BsmFI GGGAC 4 cut(s) 429, 1086, 1539, 2045
BsmI GAATGC 3 cut(s) 141, 1731, 1738
BsnI GGCC 3 cut(s) 270, 371, 627
Bso31I GGTCTC 1 cut(s) 528
Bsp119I TTCGAA 2 cut(s) 145, 151
Bsp1286I GDGCHC 1 cut(s) 1678
Bsp13I TCCGGA 1 cut(s) 338
Bsp143I GATC 8 cut(s) 433, 572, 690, 859, 880, 978, 1783, 1844
Bsp1720I GCTNAGC 2 cut(s) 1083, 1114
BspACI CCGC 2 cut(s) 212, 1232
BspANI GGCC 3 cut(s) 270, 371, 627
BspCNI CTCAG 5 cut(s) 523, 988, 1075, 2249, 2264
BspEI TCCGGA 1 cut(s) 338
BspFNI CGCG 2 cut(s) 135, 159
BspLI GGNNCC 3 cut(s) 205, 607, 1527
BspMAI CTGCAG 1 cut(s) 175
BspPI GGATC 4 cut(s) 428, 580, 698, 1839
BspQI GCTCTTC 1 cut(s) 1269
BspT104I TTCGAA 2 cut(s) 145, 151
BspT107I GGYRCC 1 cut(s) 203
BspTNI GGTCTC 1 cut(s) 528
BsrDI GCAATG 3 cut(s) 900, 1848, 1860
BsrI ACTGG 3 cut(s) 1179, 1683, 2179
BssECI CCNNGG 7 cut(s) 252, 373, 1069, 1521, 1807, 1920, 2184
BssMI GATC 8 cut(s) 433, 572, 690, 859, 880, 978, 1783, 1844
BssNI GRCGYC 1 cut(s) 204
BssSI CACGAG 1 cut(s) 1774
BssT1I CCWWGG 3 cut(s) 252, 1521, 2184
Bst2BI CACGAG 1 cut(s) 1774
Bst2UI CCWGG 2 cut(s) 624, 1070
Bst4CI ACNGT 5 cut(s) 227, 314, 364, 613, 836
Bst6I CTCTTC 8 cut(s) 444, 798, 1269, 1314, 1323, 1365, 1946, 2142
BstACI GRCGYC 1 cut(s) 204
BstAPI GCANNNNNTGC 3 cut(s) 179, 1742, 2201
BstBAI YACGTR 2 cut(s) 792, 2292
BstBI TTCGAA 2 cut(s) 145, 151
BstC8I GCNNGC 3 cut(s) 139, 384, 773
BstDEI CTNAG 7 cut(s) 510, 975, 1083, 1114, 1559, 2236, 2251
BstDSI CCRYGG 3 cut(s) 373, 1807, 1920
BstENI CCTNNNNNAGG 1 cut(s) 235
BstF5I GGATG 5 cut(s) 688, 1312, 1411, 2095, 2136
BstFNI CGCG 2 cut(s) 135, 159
BstH2I RGCGCY 2 cut(s) 207, 1751
BstHHI GCGC 4 cut(s) 137, 206, 1438, 1750
BstKTI GATC 8 cut(s) 436, 575, 693, 862, 883, 981, 1786, 1847
BstMAI GTCTC 4 cut(s) 528, 1435, 1870, 2139
BstMBI GATC 8 cut(s) 433, 572, 690, 859, 880, 978, 1783, 1844
BstNI CCWGG 2 cut(s) 624, 1070
BstNSI RCATGY 1 cut(s) 386
BstSCI CCNGG 2 cut(s) 622, 1068
BstSFI CTRYAG 3 cut(s) 171, 306, 1167
BstSNI TACGTA 1 cut(s) 792
BstUI CGCG 2 cut(s) 135, 159
BstV2I GAAGAC 2 cut(s) 75, 186
BstX2I RGATCY 3 cut(s) 572, 859, 1844
BstXI CCANNNNNNTGG 1 cut(s) 1855
BstYI RGATCY 3 cut(s) 572, 859, 1844
BsuRI GGCC 3 cut(s) 270, 371, 627
BtgI CCRYGG 3 cut(s) 373, 1807, 1920
BtsCI GGATG 5 cut(s) 688, 1312, 1411, 2095, 2136
BtsI GCAGTG 1 cut(s) 162
BtsIMutI CAGTG 5 cut(s) 162, 609, 1676, 1715, 2151
Cac8I GCNNGC 3 cut(s) 139, 384, 773
CaiI CAGNNNCTG 2 cut(s) 179, 2243
CfoI GCGC 4 cut(s) 137, 206, 1438, 1750
Cfr13I GGNCC 3 cut(s) 370, 1526, 2022
CseI GACGC 3 cut(s) 148, 1452, 2276
Csp6I GTAC 1 cut(s) 927
CviAII CATG 7 cut(s) 36, 383, 478, 768, 1642, 1711, 1910
CviQI GTAC 1 cut(s) 927
DdeI CTNAG 7 cut(s) 510, 975, 1083, 1114, 1559, 2236, 2251
DinI GGCGCC 1 cut(s) 205
DpnI GATC 8 cut(s) 435, 574, 692, 861, 882, 980, 1785, 1846
DpnII GATC 8 cut(s) 433, 572, 690, 859, 880, 978, 1783, 1844
DraI TTTAAA 1 cut(s) 940
DrdI GACNNNNNNGTC 1 cut(s) 28
DriI GACNNNNNGTC 1 cut(s) 2272
DseDI GACNNNNNNGTC 1 cut(s) 28
EaeI YGGCCR 1 cut(s) 268
Eam1104I CTCTTC 8 cut(s) 444, 798, 1269, 1314, 1323, 1365, 1946, 2142
Eam1105I GACNNNNNGTC 1 cut(s) 2272
EarI CTCTTC 8 cut(s) 444, 798, 1269, 1314, 1323, 1365, 1946, 2142
Eco105I TACGTA 1 cut(s) 792
Eco130I CCWWGG 3 cut(s) 252, 1521, 2184
Eco31I GGTCTC 1 cut(s) 528
Eco47I GGWCC 2 cut(s) 1526, 2022
Eco47III AGCGCT 1 cut(s) 1749
Eco57I CTGAAG 6 cut(s) 657, 741, 951, 1389, 1842, 2055
Eco72I CACGTG 1 cut(s) 2292
EcoNI CCTNNNNNAGG 1 cut(s) 235
EcoO109I RGGNCCY 1 cut(s) 1526
EcoRI GAATTC 2 cut(s) 147, 153
EcoRII CCWGG 2 cut(s) 622, 1068
EcoT14I CCWWGG 3 cut(s) 252, 1521, 2184
EgeI GGCGCC 1 cut(s) 205
EheI GGCGCC 1 cut(s) 205
ErhI CCWWGG 3 cut(s) 252, 1521, 2184
Esp3I CGTCTC 1 cut(s) 1435
FaeI CATG 7 cut(s) 39, 386, 481, 771, 1645, 1714, 1913
FalI AAGNNNNNCTT 2 cut(s) 764, 796
FaqI GGGAC 4 cut(s) 429, 1086, 1539, 2045
FatI CATG 7 cut(s) 35, 382, 477, 767, 1641, 1710, 1909
FokI GGATG 5 cut(s) 695, 1319, 1418, 2102, 2143
FspBI CTAG 4 cut(s) 830, 1124, 1760, 1842
GlaI GCGC 4 cut(s) 136, 205, 1437, 1749
GsaI CCCAGC 1 cut(s) 1461
HaeII RGCGCY 2 cut(s) 207, 1751
HaeIII GGCC 3 cut(s) 270, 371, 627
HapII CCGG 1 cut(s) 339
HgaI GACGC 3 cut(s) 148, 1452, 2276
HhaI GCGC 4 cut(s) 137, 206, 1438, 1750
Hin1I GRCGYC 1 cut(s) 204
Hin1II CATG 7 cut(s) 39, 386, 481, 771, 1645, 1714, 1913
Hin6I GCGC 4 cut(s) 135, 204, 1436, 1748
HinP1I GCGC 4 cut(s) 135, 204, 1436, 1748
HincII GTYRAC 1 cut(s) 2277
HindII GTYRAC 1 cut(s) 2277
HindIII AAGCTT 1 cut(s) 1412
HpaII CCGG 1 cut(s) 339
HphI GGTGA 1 cut(s) 1970
Hpy166II GTNNAC 2 cut(s) 821, 2277
Hpy188I TCNGA 7 cut(s) 513, 537, 867, 978, 1486, 1917, 2239
Hpy8I GTNNAC 2 cut(s) 821, 2277
Hpy99I CGWCG 3 cut(s) 31, 34, 135
HpyCH4III ACNGT 5 cut(s) 227, 314, 364, 613, 836
HpyCH4IV ACGT 6 cut(s) 130, 711, 791, 1008, 1969, 2291
HpyF3I CTNAG 7 cut(s) 510, 975, 1083, 1114, 1559, 2236, 2251
HpySE526I ACGT 6 cut(s) 130, 711, 791, 1008, 1969, 2291
Hsp92I GRCGYC 1 cut(s) 204
Hsp92II CATG 7 cut(s) 39, 386, 481, 771, 1645, 1714, 1913
HspAI GCGC 4 cut(s) 135, 204, 1436, 1748
KasI GGCGCC 1 cut(s) 203
Kpn2I TCCGGA 1 cut(s) 338
Kzo9I GATC 8 cut(s) 433, 572, 690, 859, 880, 978, 1783, 1844
LguI GCTCTTC 1 cut(s) 1269
LmnI GCTCC 2 cut(s) 1288, 1673
LweI GCATC 5 cut(s) 265, 1375, 1540, 1687, 2230
MaeI CTAG 4 cut(s) 830, 1124, 1760, 1842
MaeII ACGT 6 cut(s) 130, 711, 791, 1008, 1969, 2291
MaeIII GTNAC 4 cut(s) 160, 550, 1755, 1769
MalI GATC 8 cut(s) 435, 574, 692, 861, 882, 980, 1785, 1846
MboI GATC 8 cut(s) 433, 572, 690, 859, 880, 978, 1783, 1844
MflI RGATCY 3 cut(s) 572, 859, 1844
MhlI GDGCHC 1 cut(s) 1678
MlsI TGGCCA 1 cut(s) 270
MluNI TGGCCA 1 cut(s) 270
Mly113I GGCGCC 1 cut(s) 204
MlyI GAGTC 5 cut(s) 200, 502, 558, 558, 599
MmeI TCCRAC 2 cut(s) 643, 1945
Mox20I TGGCCA 1 cut(s) 270
MroI TCCGGA 1 cut(s) 338
MroXI GAANNNNTTC 1 cut(s) 1827
MscI TGGCCA 1 cut(s) 270
MseI TTAA 7 cut(s) 282, 672, 716, 729, 939, 1200, 1506
MslI CAYNNNNRTG 2 cut(s) 648, 1853
Msp20I TGGCCA 1 cut(s) 270
MspA1I CMGCKG 3 cut(s) 179, 893, 2255
MspI CCGG 1 cut(s) 339
MspR9I CCNGG 2 cut(s) 624, 1070
Mva1269I GAATGC 3 cut(s) 141, 1731, 1738
MvaI CCWGG 2 cut(s) 624, 1070
MvnI CGCG 2 cut(s) 135, 159
NarI GGCGCC 1 cut(s) 204
NdeII GATC 8 cut(s) 433, 572, 690, 859, 880, 978, 1783, 1844
NlaIII CATG 7 cut(s) 39, 386, 481, 771, 1645, 1714, 1913
NlaIV GGNNCC 3 cut(s) 205, 607, 1527
NmuCI GTSAC 2 cut(s) 160, 550
NspI RCATGY 1 cut(s) 386
NspV TTCGAA 2 cut(s) 145, 151
PaeI GCATGC 1 cut(s) 386
PciSI GCTCTTC 1 cut(s) 1269
PcsI WCGNNNNNNNCGW 1 cut(s) 26
PctI GAATGC 3 cut(s) 141, 1731, 1738
PdmI GAANNNNTTC 1 cut(s) 1827
PfeI GAWTC 5 cut(s) 488, 538, 1265, 1814, 2095
PflFI GACNNNGTC 1 cut(s) 25
PleI GAGTC 5 cut(s) 199, 502, 557, 558, 598
PluTI GGCGCC 1 cut(s) 207
PmaCI CACGTG 1 cut(s) 2292
PmlI CACGTG 1 cut(s) 2292
PpsI GAGTC 5 cut(s) 199, 502, 557, 558, 598
Ppu21I YACGTR 2 cut(s) 792, 2292
PpuMI RGGWCCY 1 cut(s) 1526
PshBI ATTAAT 1 cut(s) 672
PsiI TTATAA 1 cut(s) 1016
Psp5II RGGWCCY 1 cut(s) 1526
Psp6I CCWGG 2 cut(s) 622, 1068
PspCI CACGTG 1 cut(s) 2292
PspFI CCCAGC 1 cut(s) 1457
PspGI CCWGG 2 cut(s) 622, 1068
PspN4I GGNNCC 3 cut(s) 205, 607, 1527
PspPI GGNCC 3 cut(s) 370, 1526, 2022
PspPPI RGGWCCY 1 cut(s) 1526
PsrI GAACNNNNNNTAC 2 cut(s) 1164, 1196
PstI CTGCAG 1 cut(s) 175
PstNI CAGNNNCTG 2 cut(s) 179, 2243
PsuI RGATCY 3 cut(s) 572, 859, 1844
PsyI GACNNNGTC 1 cut(s) 25
PvuII CAGCTG 3 cut(s) 179, 893, 2255
RsaI GTAC 1 cut(s) 928
RsaNI GTAC 1 cut(s) 927
RseI CAYNNNNRTG 2 cut(s) 648, 1853
SapI GCTCTTC 1 cut(s) 1269
SaqAI TTAA 7 cut(s) 282, 672, 716, 729, 939, 1200, 1506
Sau3AI GATC 8 cut(s) 433, 572, 690, 859, 880, 978, 1783, 1844
Sau96I GGNCC 3 cut(s) 370, 1526, 2022
ScaI AGTACT 1 cut(s) 928
SchI GAGTC 5 cut(s) 200, 502, 558, 558, 599
ScrFI CCNGG 2 cut(s) 624, 1070
SduI GDGCHC 1 cut(s) 1678
SfaNI GCATC 5 cut(s) 265, 1375, 1540, 1687, 2230
SfcI CTRYAG 3 cut(s) 171, 306, 1167
SfoI GGCGCC 1 cut(s) 205
SfuI TTCGAA 2 cut(s) 145, 151
SinI GGWCC 2 cut(s) 1526, 2022
SmiMI CAYNNNNRTG 2 cut(s) 648, 1853
SmlI CTYRAG 7 cut(s) 850, 1031, 1605, 1751, 1871, 2099, 2205
SmoI CTYRAG 7 cut(s) 850, 1031, 1605, 1751, 1871, 2099, 2205
SnaBI TACGTA 1 cut(s) 792
SphI GCATGC 1 cut(s) 386
SsiI CCGC 2 cut(s) 212, 1232
SspDI GGCGCC 1 cut(s) 203
SspI AATATT 1 cut(s) 733
SspMI CTAG 4 cut(s) 830, 1124, 1760, 1842
StyD4I CCNGG 2 cut(s) 622, 1068
StyI CCWWGG 3 cut(s) 252, 1521, 2184
TaaI ACNGT 5 cut(s) 227, 314, 364, 613, 836
TaiI ACGT 6 cut(s) 133, 714, 794, 1011, 1972, 2294
TaqI TCGA 5 cut(s) 20, 145, 151, 1498, 2148
TatI WGTACW 1 cut(s) 926
TauI GCSGC 1 cut(s) 215
TfiI GAWTC 5 cut(s) 488, 538, 1265, 1814, 2095
Tru1I TTAA 7 cut(s) 282, 672, 716, 729, 939, 1200, 1506
Tru9I TTAA 7 cut(s) 282, 672, 716, 729, 939, 1200, 1506
TscAI CASTG 5 cut(s) 169, 616, 1683, 1722, 2158
TseFI GTSAC 2 cut(s) 160, 550
Tsp45I GTSAC 2 cut(s) 160, 550
TspDTI ATGAA 5 cut(s) 681, 1323, 1332, 1350, 1359
TspGWI ACGGA 4 cut(s) 249, 583, 1678, 1796
TspRI CASTG 5 cut(s) 169, 616, 1683, 1722, 2158
Tth111I GACNNNGTC 1 cut(s) 25
VpaK11BI GGWCC 2 cut(s) 1526, 2022
VspI ATTAAT 1 cut(s) 672
XagI CCTNNNNNAGG 1 cut(s) 235
XapI RAATTY 6 cut(s) 147, 153, 482, 911, 962, 2162
XbaI TCTAGA 2 cut(s) 1123, 1841
XceI RCATGY 1 cut(s) 386
XcmI CCANNNNNNNNNTGG 1 cut(s) 364
XmnI GAANNNNTTC 1 cut(s) 1827
XspI CTAG 4 cut(s) 830, 1124, 1760, 1842
ZrmI AGTACT 1 cut(s) 928
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.