Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5D

Orthogroup ID

OG0005823

Physical Location & Seq

Genomic Coordinates Reverse (-)

54046263 .. 54057766

Gene Structure 11504 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5DG361300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1956 bp

ATGGCTTTCTCACACACGTCGTCGTCGCCATGCACATCATTTCTCTCGCACCTCCAGTCTTCGTCTTCGCTGCTCAAATTACAGCACCCCCTCCTCCTCCTCTCCCAACGCCGCGCTCGCATTCGAATTCGAATTCGCGTCACGGCTTCTGCAGCAGCTGCCGTTGTTGAGTCTTCTTCAAACGGCGCCGTAGCGGCGAAACAGGACAGTTCCTACGGAAGACAGTTCTTTCCCTTGGCTGCCGTTGTTGGCCAAGATGCTATTAAAACTGCTCTGTTGCTTGGGGCTATAGACCGTGAAATTGGAGGAATTGCTATCTCCGGAAGGAGGGGAACAGCCAAAACAGTAATGGCCCGTGGTTTGCATGCAATTTTACCACCCATTGAAGTAGTCGTTGGTTCAATAGCAAATGCTGATCCAGCCTGTCCCGAAGAGTGGGAAGATGTTTTACCTGAACGCATGGAATTTGATTCTGCTGGTAATGTCAAGACTCAGATTGTTAGGTCTCCTTTTGTTCAGATTCCACTTGGAGTCACAGAGGACAGACTCATTGGATCTGTTGATGTTGAGGAGTCCGTGAGAACAGGAACCACTGTTTTCCAACCTGGCCTTCTTGCTGAAGCTCATAGAGGTGTATTATATGTTGATGAAATTAATCTTTTGGATGAGGGGATCAGTAATTTGCTTCTCAACGTATTAACTGAAGGAGTTAATATTGTTGAGAGAGAGGGAATGAGCTTTAGACACCCATGCAAGCCACTCTTGATTGCTACGTATAATCCAGAAGAGGGTGCTGTGCGTGAACACTTACTAGACCGTATTGCAATCAACTTGAGTGCAGATCTTCCGATGAGTTTTGATGATCGCGTTGCAGCTGTGGGCATTGCGACAAAATTTCAGGAATATAGTACTGAAGTCTTTAAAATGGTTCAAGAAGAAACAGAATTTGCAAAAACTCAGATCATTTTGGCAAGAGAGTATCTAAAGGACGTTGTTATAAGCAGAGAACAACTCAAGTATCTGGTTTTGGAAGCCCTGCGAGGTGGTTGCCAGGGACATAGAGCTGAGCTATATGCTTCCCGTGTTGCAAAATGCTTAGCTGCTCTAGATGGACGAGAAAAAGTTTATGCTGATGACCTGAAAAAAGCTGTAGAACTGGTCATACTTCCTCGCTCAATCATTAATGAGAACCCACCAGAACAGCAAAATCAACCGCCTCCACCTCCACCACCTCCTCAAAATCAAGAATCTGGGGAAGAGCAAAATGAGGAGCAAGAAGAAAAAGAGGATGACAATGATGAAGAGAAGGAAGAGAATGAAGATAATGAACAAGAGCAGGAACAGCTACCTGAAGAGTTTGTCTTTGATGCAGAAGGGGGTTTGGTGGATGAGAAGCTTCTTTTTTTTGCACAACAAGCGCAGAGACGCAAAGGGAAGGCTGGGAGAGCAAAGAATGTTATCTTTTCAGAGGATAGAGGTCGATACATTAAGCCAATGCTTCCAAAGGGACCTGTAAAGAGATTAGCTGTTGATGCAACTCTTAGAGTTGCTGCACCATATCAAAAATTGCGTAGGGAGAAGAACACTCAAGTCGGTAGGAAAGTTTTTGTTGAAAAAACTGACATGAGAGCAAAGAGAATGGCACGGAAAGCAGGAGCACTGGTTATATTTGTTGTTGATGCAAGTGGAAGCATGGCACTGAACCGAATGCAGAATGCAAAAGGTGCAGCGCTCAAGTTACTAGCTGAAAGTTATACGAGCAGAGATCAGGTTTCTATTATTCCTTTCCGTGGAGATTCTGCTGAAGTTCTTCTGCCTCCTTCTAGATCCATTGCAATGGCAAGAAATCGTCTTGAGAGACTTCCCTGTGGTGGAGGTTCTCCCCTTGCTCATGGTCTGACCACGGTATGTAGATCCATTTACACACACCAAAGGAAAAAAAAAAAAAGGGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009532 GO:0009534 GO:0009536 GO:0009570 GO:0009579 GO:0010007 GO:0031976 GO:0031984 GO:0032991 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:1902494

KEGG Pathways

Metabolic & Signaling

ko00860 ko01100 ko01110 map00860 map01100 map01110

Pfam Domains

Protein Families

Mg_chelatase VWA_2

Protein Analysis

651

Amino Acids

71.9

Weight (kDa)

6.62

Isoelectric Point (pI)

49.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Mg_chelatase	PF01078	196 - 263	7.8e-07	Magnesium chelatase, subunit ChlI
AAA_lid_2	PF17863	343 - 403	1.2e-13	AAA lid domain
VWA_2	PF13519	555 - 649	9.3e-12	von Willebrand factor type A domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005823)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G08520
fragaria_vesca	FvH4_3g28280 FvH4_3g28280 FvH4_3g28280 FvH4_3g28280 FvH4_3g28280 FvH4_3g28280 FvH4_3g28280 FvH4_3g28280 FvH4_3g28280 FvH4_3g28280 FvH4_3g28280
malus_domestica	MD03G1170000.v1.1 MD11G1187400.v1.1
prunus_persica	Prupe.4G257100_v2.0.a1 Prupe.4G257100_v2.0.a1
pyrus_communis	pycom11g16410 pycom11g16420
rosa_chinensis	RchiOBHm_Chr5g0051161
rosa_laevigata	RLG00000034758
rosa_multiflora	Rmu_sc0000446.1_g000012
rosa_roxburghii	Rroxscaffold_1G00029290
rosa_samantha	Rh5AG338500 Rh5BG348400 Rh5CG375500 Rh5DG361300
rosa_wichuraiana	Rw0G023660 Rw5G031970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	998
AccB1I	GGYRCC	1 cut(s)	185
AccII	CGCG	3 cut(s)	114, 138, 867
AccIII	TCCGGA	1 cut(s)	320
AciI	CCGC	3 cut(s)	112, 194, 1214
AclWI	GGATC	5 cut(s)	410, 562, 680, 1821, 1908
AcoI	YGGCCR	1 cut(s)	250
AcsI	RAATTY	5 cut(s)	126, 132, 464, 893, 944
AcuI	CTGAAG	5 cut(s)	639, 723, 933, 1371, 1824
AcyI	GRCGYC	1 cut(s)	186
AfaI	GTAC	1 cut(s)	910
AfeI	AGCGCT	1 cut(s)	1731
AfiI	CCNNNNNNNGG	5 cut(s)	327, 435, 788, 1790, 1871
AflIII	ACRYGT	1 cut(s)	15
AgsI	TTSAA	5 cut(s)	180, 386, 402, 932, 1613
AjiI	CACGTC	1 cut(s)	18
AjnI	CCWGG	2 cut(s)	604, 1050
AjuI	GAANNNNNNNTTGG	4 cut(s)	378, 410, 594, 626
Alw21I	GWGCWC	1 cut(s)	1660
Alw26I	GTCTC	3 cut(s)	510, 1417, 1852
AlwI	GGATC	5 cut(s)	410, 562, 680, 1821, 1908
AlwNI	CAGNNNCTG	1 cut(s)	158
Aor13HI	TCCGGA	1 cut(s)	320
Aor51HI	AGCGCT	1 cut(s)	1731
AoxI	GGCC	3 cut(s)	250, 351, 607
ApeKI	GCWGC	9 cut(s)	70, 152, 155, 158, 239, 872, 1100, 1550, 1727
ApoI	RAATTY	5 cut(s)	126, 132, 464, 893, 944
AseI	ATTAAT	2 cut(s)	654, 1182
Asp700I	GAANNNNTTC	1 cut(s)	1809
AspLEI	GCGC	4 cut(s)	116, 188, 1420, 1732
AspS9I	GGNCC	2 cut(s)	352, 1508
AsuII	TTCGAA	2 cut(s)	124, 130
AvaII	GGWCC	1 cut(s)	1508
BaeI	ACNNNNGTAYC	2 cut(s)	1001, 1034
BalI	TGGCCA	1 cut(s)	252
BanI	GGYRCC	1 cut(s)	185
BarI	GAAGNNNNNNTAC	2 cut(s)	432, 464
BbsI	GAAGAC	4 cut(s)	51, 57, 165, 226
Bbv12I	GWGCWC	1 cut(s)	1660
BbvI	GCAGC	9 cut(s)	57, 145, 164, 167, 226, 884, 1087, 1537, 1739
BccI	CCATC	1 cut(s)	1103
BceAI	ACGGC	5 cut(s)	146, 159, 173, 199, 227
BcgI	CGANNNNNNTGC	2 cut(s)	1029, 1063
BciT130I	CCWGG	2 cut(s)	606, 1052
BcoDI	GTCTC	3 cut(s)	510, 1417, 1852
BfaI	CTAG	4 cut(s)	812, 1106, 1742, 1824
BfmI	CTRYAG	3 cut(s)	150, 288, 1149
BfoI	RGCGCY	2 cut(s)	189, 1733
BglI	GCCNNNNNGGC	1 cut(s)	194
BglII	AGATCT	1 cut(s)	841
BlpI	GCTNAGC	2 cut(s)	1065, 1096
BmcAI	AGTACT	1 cut(s)	910
Bme1390I	CCNGG	2 cut(s)	606, 1052
Bme18I	GGWCC	1 cut(s)	1508
BmgBI	CACGTC	1 cut(s)	18
BmgT120I	GGNCC	2 cut(s)	352, 1508
BmiI	GGNNCC	3 cut(s)	187, 589, 1509
BmrFI	CCNGG	2 cut(s)	606, 1052
BmsI	GCATC	4 cut(s)	247, 1357, 1522, 1669
BpiI	GAAGAC	4 cut(s)	51, 57, 165, 226
BplI	GAGNNNNNCTC	2 cut(s)	996, 1028
BpmI	CTGGAG	1 cut(s)	38
Bpu1102I	GCTNAGC	2 cut(s)	1065, 1096
Bpu14I	TTCGAA	2 cut(s)	124, 130
BpuEI	CTTGAG	5 cut(s)	853, 998, 1572, 1718, 1874
BsaAI	YACGTR	1 cut(s)	774
BsaHI	GRCGYC	1 cut(s)	186
BsaI	GGTCTC	1 cut(s)	510
BsaJI	CCNNGG	5 cut(s)	234, 355, 1051, 1789, 1902
BsaWI	WCCGGW	1 cut(s)	320
BsaXI	ACNNNNNCTCC	2 cut(s)	1647, 1677
Bsc4I	CCNNNNNNNGG	5 cut(s)	327, 435, 788, 1790, 1871
Bse1I	ACTGG	3 cut(s)	55, 1161, 1665
Bse3DI	GCAATG	3 cut(s)	882, 1830, 1842
BseAI	TCCGGA	1 cut(s)	320
BseBI	CCWGG	2 cut(s)	606, 1052
BseDI	CCNNGG	5 cut(s)	234, 355, 1051, 1789, 1902
BseGI	GGATG	3 cut(s)	670, 1294, 1393
BseLI	CCNNNNNNNGG	5 cut(s)	327, 435, 788, 1790, 1871
BseMI	GCAATG	3 cut(s)	882, 1830, 1842
BseMII	CTCAG	3 cut(s)	506, 971, 1056
BseNI	ACTGG	3 cut(s)	55, 1161, 1665
BseRI	GAGGAG	6 cut(s)	83, 86, 89, 584, 1224, 1283
BseXI	GCAGC	9 cut(s)	57, 145, 164, 167, 226, 884, 1087, 1537, 1739
BseYI	CCCAGC	1 cut(s)	1439
BsgI	GTGCAG	3 cut(s)	858, 1536, 1746
Bsh1236I	CGCG	3 cut(s)	114, 138, 867
BshFI	GGCC	3 cut(s)	252, 353, 609
BshNI	GGYRCC	1 cut(s)	185
BsiHKAI	GWGCWC	1 cut(s)	1660
BsiSI	CCGG	1 cut(s)	321
BslFI	GGGAC	3 cut(s)	411, 1068, 1521
BslI	CCNNNNNNNGG	5 cut(s)	327, 435, 788, 1790, 1871
BsmAI	GTCTC	3 cut(s)	510, 1417, 1852
BsmBI	CGTCTC	1 cut(s)	1417
BsmFI	GGGAC	3 cut(s)	411, 1068, 1521
BsmI	GAATGC	3 cut(s)	120, 1713, 1720
BsnI	GGCC	3 cut(s)	252, 353, 609
Bso31I	GGTCTC	1 cut(s)	510
Bsp119I	TTCGAA	2 cut(s)	124, 130
Bsp1286I	GDGCHC	1 cut(s)	1660
Bsp13I	TCCGGA	1 cut(s)	320
Bsp143I	GATC	9 cut(s)	415, 554, 672, 841, 862, 960, 1765, 1826, 1913
Bsp1720I	GCTNAGC	2 cut(s)	1065, 1096
BspACI	CCGC	3 cut(s)	112, 194, 1214
BspANI	GGCC	3 cut(s)	252, 353, 609
BspCNI	CTCAG	3 cut(s)	505, 970, 1057
BspEI	TCCGGA	1 cut(s)	320
BspFNI	CGCG	3 cut(s)	114, 138, 867
BspLI	GGNNCC	3 cut(s)	187, 589, 1509
BspMAI	CTGCAG	1 cut(s)	154
BspPI	GGATC	5 cut(s)	410, 562, 680, 1821, 1908
BspQI	GCTCTTC	1 cut(s)	1251
BspT104I	TTCGAA	2 cut(s)	124, 130
BspT107I	GGYRCC	1 cut(s)	185
BspTNI	GGTCTC	1 cut(s)	510
BsrDI	GCAATG	3 cut(s)	882, 1830, 1842
BsrI	ACTGG	3 cut(s)	55, 1161, 1665
BssECI	CCNNGG	5 cut(s)	234, 355, 1051, 1789, 1902
BssMI	GATC	9 cut(s)	415, 554, 672, 841, 862, 960, 1765, 1826, 1913
BssNI	GRCGYC	1 cut(s)	186
BssT1I	CCWWGG	1 cut(s)	234
Bst2UI	CCWGG	2 cut(s)	606, 1052
Bst4CI	ACNGT	7 cut(s)	209, 225, 296, 346, 595, 818, 1906
Bst6I	CTCTTC	6 cut(s)	426, 780, 1251, 1296, 1305, 1347
BstACI	GRCGYC	1 cut(s)	186
BstAPI	GCANNNNNTGC	2 cut(s)	158, 1724
BstBAI	YACGTR	1 cut(s)	774
BstBI	TTCGAA	2 cut(s)	124, 130
BstC8I	GCNNGC	3 cut(s)	118, 366, 755
BstDEI	CTNAG	5 cut(s)	492, 957, 1065, 1096, 1541
BstDSI	CCRYGG	3 cut(s)	355, 1789, 1902
BstF5I	GGATG	3 cut(s)	670, 1294, 1393
BstFNI	CGCG	3 cut(s)	114, 138, 867
BstH2I	RGCGCY	2 cut(s)	189, 1733
BstHHI	GCGC	4 cut(s)	116, 188, 1420, 1732
BstKTI	GATC	9 cut(s)	418, 557, 675, 844, 865, 963, 1768, 1829, 1916
BstMAI	GTCTC	3 cut(s)	510, 1417, 1852
BstMBI	GATC	9 cut(s)	415, 554, 672, 841, 862, 960, 1765, 1826, 1913
BstNI	CCWGG	2 cut(s)	606, 1052
BstNSI	RCATGY	1 cut(s)	368
BstSCI	CCNGG	2 cut(s)	604, 1050
BstSFI	CTRYAG	3 cut(s)	150, 288, 1149
BstSNI	TACGTA	1 cut(s)	774
BstUI	CGCG	3 cut(s)	114, 138, 867
BstV1I	GCAGC	9 cut(s)	57, 145, 164, 167, 226, 884, 1087, 1537, 1739
BstV2I	GAAGAC	4 cut(s)	51, 57, 165, 226
BstX2I	RGATCY	4 cut(s)	554, 841, 1826, 1913
BstXI	CCANNNNNNTGG	1 cut(s)	1837
BstYI	RGATCY	4 cut(s)	554, 841, 1826, 1913
BsuRI	GGCC	3 cut(s)	252, 353, 609
BtgI	CCRYGG	3 cut(s)	355, 1789, 1902
BtrI	CACGTC	1 cut(s)	18
BtsCI	GGATG	3 cut(s)	670, 1294, 1393
BtsIMutI	CAGTG	3 cut(s)	591, 1658, 1697
Cac8I	GCNNGC	3 cut(s)	118, 366, 755
CaiI	CAGNNNCTG	1 cut(s)	158
CfoI	GCGC	4 cut(s)	116, 188, 1420, 1732
Cfr13I	GGNCC	2 cut(s)	352, 1508
CseI	GACGC	2 cut(s)	127, 1434
Csp6I	GTAC	1 cut(s)	909
CviAII	CATG	7 cut(s)	30, 365, 460, 750, 1624, 1693, 1892
CviQI	GTAC	1 cut(s)	909
DdeI	CTNAG	5 cut(s)	492, 957, 1065, 1096, 1541
DinI	GGCGCC	1 cut(s)	187
DpnI	GATC	9 cut(s)	417, 556, 674, 843, 864, 962, 1767, 1828, 1915
DpnII	GATC	9 cut(s)	415, 554, 672, 841, 862, 960, 1765, 1826, 1913
DraI	TTTAAA	1 cut(s)	922
EaeI	YGGCCR	1 cut(s)	250
Eam1104I	CTCTTC	6 cut(s)	426, 780, 1251, 1296, 1305, 1347
EarI	CTCTTC	6 cut(s)	426, 780, 1251, 1296, 1305, 1347
Eco105I	TACGTA	1 cut(s)	774
Eco130I	CCWWGG	1 cut(s)	234
Eco31I	GGTCTC	1 cut(s)	510
Eco47I	GGWCC	1 cut(s)	1508
Eco47III	AGCGCT	1 cut(s)	1731
Eco57I	CTGAAG	5 cut(s)	639, 723, 933, 1371, 1824
EcoO109I	RGGNCCY	1 cut(s)	1508
EcoRI	GAATTC	2 cut(s)	126, 132
EcoRII	CCWGG	2 cut(s)	604, 1050
EcoT14I	CCWWGG	1 cut(s)	234
EgeI	GGCGCC	1 cut(s)	187
EheI	GGCGCC	1 cut(s)	187
ErhI	CCWWGG	1 cut(s)	234
Esp3I	CGTCTC	1 cut(s)	1417
FaeI	CATG	7 cut(s)	33, 368, 463, 753, 1627, 1696, 1895
FalI	AAGNNNNNCTT	2 cut(s)	746, 778
FaqI	GGGAC	3 cut(s)	411, 1068, 1521
FatI	CATG	7 cut(s)	29, 364, 459, 749, 1623, 1692, 1891
FokI	GGATG	3 cut(s)	677, 1301, 1400
FspBI	CTAG	4 cut(s)	812, 1106, 1742, 1824
GlaI	GCGC	4 cut(s)	115, 187, 1419, 1731
GsaI	CCCAGC	1 cut(s)	1443
GsuI	CTGGAG	1 cut(s)	38
HaeII	RGCGCY	2 cut(s)	189, 1733
HaeIII	GGCC	3 cut(s)	252, 353, 609
HapII	CCGG	1 cut(s)	321
HgaI	GACGC	2 cut(s)	127, 1434
HhaI	GCGC	4 cut(s)	116, 188, 1420, 1732
Hin1I	GRCGYC	1 cut(s)	186
Hin1II	CATG	7 cut(s)	33, 368, 463, 753, 1627, 1696, 1895
Hin6I	GCGC	4 cut(s)	114, 186, 1418, 1730
HinP1I	GCGC	4 cut(s)	114, 186, 1418, 1730
HindIII	AAGCTT	1 cut(s)	1394
HinfI	GANTC	9 cut(s)	170, 470, 490, 520, 531, 546, 572, 1247, 1796
HpaII	CCGG	1 cut(s)	321
Hpy166II	GTNNAC	1 cut(s)	803
Hpy188I	TCNGA	6 cut(s)	495, 519, 849, 960, 1468, 1899
Hpy8I	GTNNAC	1 cut(s)	803
Hpy99I	CGWCG	3 cut(s)	22, 25, 28
HpyAV	CCTTC	7 cut(s)	318, 620, 698, 1300, 1367, 1429, 1830
HpyCH4III	ACNGT	7 cut(s)	209, 225, 296, 346, 595, 818, 1906
HpyCH4IV	ACGT	4 cut(s)	17, 693, 773, 990
HpyF3I	CTNAG	5 cut(s)	492, 957, 1065, 1096, 1541
HpySE526I	ACGT	4 cut(s)	17, 693, 773, 990
Hsp92I	GRCGYC	1 cut(s)	186
Hsp92II	CATG	7 cut(s)	33, 368, 463, 753, 1627, 1696, 1895
HspAI	GCGC	4 cut(s)	114, 186, 1418, 1730
KasI	GGCGCC	1 cut(s)	185
Kpn2I	TCCGGA	1 cut(s)	320
Kzo9I	GATC	9 cut(s)	415, 554, 672, 841, 862, 960, 1765, 1826, 1913
LguI	GCTCTTC	1 cut(s)	1251
LmnI	GCTCC	2 cut(s)	1270, 1655
Lsp1109I	GCAGC	9 cut(s)	57, 145, 164, 167, 226, 884, 1087, 1537, 1739
LweI	GCATC	4 cut(s)	247, 1357, 1522, 1669
MaeI	CTAG	4 cut(s)	812, 1106, 1742, 1824
MaeII	ACGT	4 cut(s)	17, 693, 773, 990
MaeIII	GTNAC	3 cut(s)	139, 532, 1737
MalI	GATC	9 cut(s)	417, 556, 674, 843, 864, 962, 1767, 1828, 1915
MboI	GATC	9 cut(s)	415, 554, 672, 841, 862, 960, 1765, 1826, 1913
MflI	RGATCY	4 cut(s)	554, 841, 1826, 1913
MhlI	GDGCHC	1 cut(s)	1660
MlsI	TGGCCA	1 cut(s)	252
MluNI	TGGCCA	1 cut(s)	252
Mly113I	GGCGCC	1 cut(s)	186
MlyI	GAGTC	5 cut(s)	179, 484, 540, 540, 581
MmeI	TCCRAC	1 cut(s)	625
Mox20I	TGGCCA	1 cut(s)	252
MroI	TCCGGA	1 cut(s)	320
MroXI	GAANNNNTTC	1 cut(s)	1809
MscI	TGGCCA	1 cut(s)	252
MseI	TTAA	7 cut(s)	264, 654, 698, 711, 921, 1182, 1488
MslI	CAYNNNNRTG	2 cut(s)	630, 1835
Msp20I	TGGCCA	1 cut(s)	252
MspA1I	CMGCKG	2 cut(s)	158, 875
MspI	CCGG	1 cut(s)	321
MspR9I	CCNGG	2 cut(s)	606, 1052
Mva1269I	GAATGC	3 cut(s)	120, 1713, 1720
MvaI	CCWGG	2 cut(s)	606, 1052
MvnI	CGCG	3 cut(s)	114, 138, 867
NarI	GGCGCC	1 cut(s)	186
NdeII	GATC	9 cut(s)	415, 554, 672, 841, 862, 960, 1765, 1826, 1913
NlaIII	CATG	7 cut(s)	33, 368, 463, 753, 1627, 1696, 1895
NlaIV	GGNNCC	3 cut(s)	187, 589, 1509
NmuCI	GTSAC	2 cut(s)	139, 532
NspI	RCATGY	1 cut(s)	368
NspV	TTCGAA	2 cut(s)	124, 130
PaeI	GCATGC	1 cut(s)	368
PciSI	GCTCTTC	1 cut(s)	1251
PctI	GAATGC	3 cut(s)	120, 1713, 1720
PdmI	GAANNNNTTC	1 cut(s)	1809
PfeI	GAWTC	4 cut(s)	470, 520, 1247, 1796
PleI	GAGTC	5 cut(s)	178, 484, 539, 540, 580
PluTI	GGCGCC	1 cut(s)	189
PpsI	GAGTC	5 cut(s)	178, 484, 539, 540, 580
Ppu21I	YACGTR	1 cut(s)	774
PpuMI	RGGWCCY	1 cut(s)	1508
PshBI	ATTAAT	2 cut(s)	654, 1182
PsiI	TTATAA	1 cut(s)	998
Psp5II	RGGWCCY	1 cut(s)	1508
Psp6I	CCWGG	2 cut(s)	604, 1050
PspFI	CCCAGC	1 cut(s)	1439
PspGI	CCWGG	2 cut(s)	604, 1050
PspN4I	GGNNCC	3 cut(s)	187, 589, 1509
PspPI	GGNCC	2 cut(s)	352, 1508
PspPPI	RGGWCCY	1 cut(s)	1508
PsrI	GAACNNNNNNTAC	2 cut(s)	1146, 1178
PstI	CTGCAG	1 cut(s)	154
PstNI	CAGNNNCTG	1 cut(s)	158
PsuI	RGATCY	4 cut(s)	554, 841, 1826, 1913
PvuII	CAGCTG	2 cut(s)	158, 875
RsaI	GTAC	1 cut(s)	910
RsaNI	GTAC	1 cut(s)	909
RseI	CAYNNNNRTG	2 cut(s)	630, 1835
SapI	GCTCTTC	1 cut(s)	1251
SaqAI	TTAA	7 cut(s)	264, 654, 698, 711, 921, 1182, 1488
Sau3AI	GATC	9 cut(s)	415, 554, 672, 841, 862, 960, 1765, 1826, 1913
Sau96I	GGNCC	2 cut(s)	352, 1508
ScaI	AGTACT	1 cut(s)	910
SchI	GAGTC	5 cut(s)	179, 484, 540, 540, 581
ScrFI	CCNGG	2 cut(s)	606, 1052
SduI	GDGCHC	1 cut(s)	1660
SfaNI	GCATC	4 cut(s)	247, 1357, 1522, 1669
SfcI	CTRYAG	3 cut(s)	150, 288, 1149
SfoI	GGCGCC	1 cut(s)	187
SfuI	TTCGAA	2 cut(s)	124, 130
SinI	GGWCC	1 cut(s)	1508
SmiMI	CAYNNNNRTG	2 cut(s)	630, 1835
SmlI	CTYRAG	5 cut(s)	832, 1013, 1587, 1733, 1853
SmoI	CTYRAG	5 cut(s)	832, 1013, 1587, 1733, 1853
SnaBI	TACGTA	1 cut(s)	774
SphI	GCATGC	1 cut(s)	368
SsiI	CCGC	3 cut(s)	112, 194, 1214
SspDI	GGCGCC	1 cut(s)	185
SspI	AATATT	1 cut(s)	715
SspMI	CTAG	4 cut(s)	812, 1106, 1742, 1824
StyD4I	CCNGG	2 cut(s)	604, 1050
StyI	CCWWGG	1 cut(s)	234
TaaI	ACNGT	7 cut(s)	209, 225, 296, 346, 595, 818, 1906
TaiI	ACGT	4 cut(s)	20, 696, 776, 993
TaqI	TCGA	3 cut(s)	124, 130, 1480
TatI	WGTACW	1 cut(s)	908
TauI	GCSGC	2 cut(s)	114, 197
TfiI	GAWTC	4 cut(s)	470, 520, 1247, 1796
Tru1I	TTAA	7 cut(s)	264, 654, 698, 711, 921, 1182, 1488
Tru9I	TTAA	7 cut(s)	264, 654, 698, 711, 921, 1182, 1488
TscAI	CASTG	3 cut(s)	598, 1665, 1704
TseFI	GTSAC	2 cut(s)	139, 532
TseI	GCWGC	9 cut(s)	70, 152, 155, 158, 239, 872, 1100, 1550, 1727
Tsp45I	GTSAC	2 cut(s)	139, 532
TspDTI	ATGAA	4 cut(s)	663, 1314, 1332, 1341
TspGWI	ACGGA	4 cut(s)	231, 565, 1660, 1778
TspRI	CASTG	3 cut(s)	598, 1665, 1704
VpaK11BI	GGWCC	1 cut(s)	1508
VspI	ATTAAT	2 cut(s)	654, 1182
XapI	RAATTY	5 cut(s)	126, 132, 464, 893, 944
XbaI	TCTAGA	2 cut(s)	1105, 1823
XceI	RCATGY	1 cut(s)	368
XcmI	CCANNNNNNNNNTGG	1 cut(s)	346
XmnI	GAANNNNTTC	1 cut(s)	1809
XspI	CTAG	4 cut(s)	812, 1106, 1742, 1824
ZrmI	AGTACT	1 cut(s)	910

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.