RchiOBHm_Chr5g0051161

Involved in chlorophyll biosynthesis. Catalyzes the insertion of magnesium ion into protoporphyrin IX to yield Mg- protoporphyrin IX

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0005823
Physical Location & Seq
Reverse (-)
52099401 .. 52109841
10441 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ32865

Sequence Viewer

Length: 2277 bp
ATGGCTTTCTCACACACGTCGTCGTCGCCATGCACATCATTTCTCTCGCACCTCCAGTCTTCGTCTTCGCTGCTCAAATTACAGCACCCCCTCCTCCTCCTCTCCCAACGCCGCGCTCGCATTCGAATTCGAATTCGCGTCACGGCTTCTGCAGCAGCTGCCGTTGTTGAGTCTTCTTCAAACGGCGCCGTAGCGGCGAAACAGGACAGTTCCTACGGAAGACAGTTCTTTCCCTTGGCTGCCGTTGTTGGCCAAGATGCTATTAAAACTGCTCTGTTGCTTGGGGCTATAGACCGTGAAATTGGAGGAATTGCTATCTCCGGAAGGAGGGGAACAGCCAAAACAGTAATGGCCCGTGGTTTGCATGCAATTTTACCACCCATTGAAGTAGTCGTTGGTTCAATAGCAAATGCTGATCCAGCCTGTCCCGAAGAGTGGGAAGATGTTTTACCTGAACGCATGGAATTTGATTCTGCTGGTAATGTCAAGACTCAGATTGTTAGGTCTCCTTTTGTTCAGATTCCACTTGGAGTCACAGAGGACAGACTCATTGGATCTGTTGATGTTGAGGAGTCCGTGAGAACAGGAACCACTGTTTTCCAACCTGGCCTTCTTGCTGAAGCTCATAGAGGTGTATTATATGTTGATGAAATTAATCTTTTGGATGAGGGGATCAGTAATTTGCTTCTCAACGTATTAACTGAAGGAGTTAATATTGTTGAGAGAGAGGGAATGAGCTTTAGACACCCATGCAAGCCACTCTTGATTGCTACGTATAATCCAGAAGAGGGTGCTGTGCGTGAACACTTACTAGACCGTATTGCAATCAACTTGAGTGCAGATCTTCCGATGAGTTTTGATGATCGCGTTGCAGCTGTGGGCATTGCGACAAAATTTCAGGAATATAGTACTGAAGTCTTTAAAATGGTTCAAGAAGAAACAGAATTTGCAAAAACTCAGATCATTTTGGCAAGAGAGTATCTAAAGGACGTTGTTATAAGCAGAGAACAACTCAAGTATCTGGTTTTGGAAGCCCTGCGAGGTGGTTGCCAGGGACATAGAGCTGAGCTATATGCTTCCCGTGTTGCAAAATGCTTAGCTGCTCTAGATGGACGAGAAAAAGTTTATGCTGATGACCTGAAAAAAGCTGTAGAACTGGTCATACTTCCTCGCTCAATCATTAATGAGAACCCACCAGAACAGCAAAATCAACCGCCTCCACCTCCACCACCTCCTCAAAATCAAGAATCTGGGGAAGAGCAAAATGAGGAGCAAGAAGAAAAAGAGGATGACAATGATGAAGAGAAGGAAGAGAATGAAGATAATGAACAAGAGCAGGAACAGCTACCTGAAGAGTTTGTCTTTGATGCAGAAGGGGGTTTGGTGGATGAGAAGCTTCTTTTTTTTGCACAACAAGCGCAGAGACGCAAAGGGAAGGCTGGGAGAGCAAAGAATGTTATCTTTTCAGAGGATAGAGGTCGATACATTAAGCCAATGCTTCCAAAGGGACCTGTAAAGAGATTAGCTGTTGATGCAACTCTTAGAGTTGCTGCACCATATCAAAAATTGCGTAGGGAGAAGAACACTCAAGTCGGTAGGAAAGTTTTTGTTGAAAAAACTGACATGAGAGCAAAGAGAATGGCACGGAAAGCAGGAGCACTGGTTATATTTGTTGTTGATGCAAGTGGAAGCATGGCACTGAACCGAATGCAGAATGCAAAAGGTGCAGCGCTCAAGTTACTAGCTGAAAGTTATACGAGCAGAGATCAGGTTTCTATTATTCCTTTCCGTGGAGATTCTGCTGAAGTTCTTCTGCCTCCTTCTAGATCCATTGCAATGGCAAGAAATCGTCTTGAGAGACTTCCCTGTGGTGGAGGTTCTCCCCTTGCTCATGGTCTGACCACGGCTGTAAGGGTTGGGCTAAATGCAGAGAAGAGTGGTGATGTTGGACGTATAATGATTGTTGCAATAACTGATGGTAGAGCCAATATATCACTAAAAAGGTCCAATGACCCTGAAGCTGCTGCTGCTGCTACTGATGTCCCAAAACCTTCGGCAAAGGAGTTGAAGGATGAGATTCTTGAGGTTGCTGGTAAAATATACAAAGCAGGGATGTCTCTTCTTGTCATCGACACTGAAAATAAATTTGTTTCAACTGGTTTTGCCAAGGAGATTGCAAGAGTTGCTCAAGGAAAATACTATTACTTGCCAAATGCCTCAGATGCTGTAATCTCAGCTGCCACAAAGGACGCTTTGTCAACATTGAAAAACACATGA

Protein Analysis

758

Amino Acids

82.84

Weight (kDa)

5.96

Isoelectric Point (pI)

45.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mg_chelatase PF01078 196 - 263 9.7e-07 Magnesium chelatase, subunit ChlI
AAA_lid_2 PF17863 343 - 403 1.4e-13 AAA lid domain
VWA_2 PF13519 555 - 660 1.6e-12 von Willebrand factor type A domain
VWA PF00092 555 - 676 4.1e-06 von Willebrand factor type A domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 998
AccB1I GGYRCC 1 cut(s) 185
AccII CGCG 3 cut(s) 114, 138, 867
AccIII TCCGGA 1 cut(s) 320
AciI CCGC 3 cut(s) 112, 194, 1214
AclWI GGATC 4 cut(s) 410, 562, 680, 1821
AcoI YGGCCR 1 cut(s) 250
AcsI RAATTY 6 cut(s) 126, 132, 464, 893, 944, 2144
AcuI CTGAAG 6 cut(s) 639, 723, 933, 1371, 1824, 2037
AcyI GRCGYC 1 cut(s) 186
AfaI GTAC 1 cut(s) 910
AfeI AGCGCT 1 cut(s) 1731
AfiI CCNNNNNNNGG 5 cut(s) 327, 435, 788, 1790, 1871
AflIII ACRYGT 1 cut(s) 15
AgsI TTSAA 8 cut(s) 180, 386, 402, 932, 1613, 2068, 2154, 2266
AhdI GACNNNNNGTC 1 cut(s) 2254
AjiI CACGTC 1 cut(s) 18
AjnI CCWGG 2 cut(s) 604, 1050
AjuI GAANNNNNNNTTGG 4 cut(s) 378, 410, 594, 626
Alw21I GWGCWC 1 cut(s) 1660
Alw26I GTCTC 4 cut(s) 510, 1417, 1852, 2121
AlwI GGATC 4 cut(s) 410, 562, 680, 1821
AlwNI CAGNNNCTG 2 cut(s) 158, 2225
Aor13HI TCCGGA 1 cut(s) 320
Aor51HI AGCGCT 1 cut(s) 1731
AoxI GGCC 3 cut(s) 250, 351, 607
ApoI RAATTY 6 cut(s) 126, 132, 464, 893, 944, 2144
AseI ATTAAT 2 cut(s) 654, 1182
Asp700I GAANNNNTTC 1 cut(s) 1809
AspLEI GCGC 4 cut(s) 116, 188, 1420, 1732
AspS9I GGNCC 3 cut(s) 352, 1508, 2004
AsuHPI GGTGA 1 cut(s) 1952
AsuII TTCGAA 2 cut(s) 124, 130
AvaII GGWCC 2 cut(s) 1508, 2004
BaeI ACNNNNGTAYC 2 cut(s) 1001, 1034
BalI TGGCCA 1 cut(s) 252
BanI GGYRCC 1 cut(s) 185
BarI GAAGNNNNNNTAC 2 cut(s) 432, 464
BbsI GAAGAC 4 cut(s) 51, 57, 165, 226
Bbv12I GWGCWC 1 cut(s) 1660
BccI CCATC 2 cut(s) 1103, 1970
BceAI ACGGC 6 cut(s) 146, 159, 173, 199, 227, 1920
BcgI CGANNNNNNTGC 2 cut(s) 1029, 1063
BciT130I CCWGG 2 cut(s) 606, 1052
BcoDI GTCTC 4 cut(s) 510, 1417, 1852, 2121
BfaI CTAG 4 cut(s) 812, 1106, 1742, 1824
BfmI CTRYAG 3 cut(s) 150, 288, 1149
BfoI RGCGCY 2 cut(s) 189, 1733
BglI GCCNNNNNGGC 1 cut(s) 194
BglII AGATCT 1 cut(s) 841
BlpI GCTNAGC 2 cut(s) 1065, 1096
BmcAI AGTACT 1 cut(s) 910
Bme1390I CCNGG 2 cut(s) 606, 1052
Bme18I GGWCC 2 cut(s) 1508, 2004
BmeRI GACNNNNNGTC 1 cut(s) 2254
BmgBI CACGTC 1 cut(s) 18
BmgT120I GGNCC 3 cut(s) 352, 1508, 2004
BmiI GGNNCC 3 cut(s) 187, 589, 1509
BmrFI CCNGG 2 cut(s) 606, 1052
BmsI GCATC 5 cut(s) 247, 1357, 1522, 1669, 2212
BpiI GAAGAC 4 cut(s) 51, 57, 165, 226
BplI GAGNNNNNCTC 2 cut(s) 996, 1028
BpmI CTGGAG 1 cut(s) 38
Bpu1102I GCTNAGC 2 cut(s) 1065, 1096
Bpu14I TTCGAA 2 cut(s) 124, 130
BpuEI CTTGAG 7 cut(s) 853, 998, 1572, 1718, 1874, 2102, 2172
BsaAI YACGTR 1 cut(s) 774
BsaHI GRCGYC 1 cut(s) 186
BsaI GGTCTC 1 cut(s) 510
BsaJI CCNNGG 6 cut(s) 234, 355, 1051, 1789, 1902, 2166
BsaWI WCCGGW 1 cut(s) 320
BsaXI ACNNNNNCTCC 2 cut(s) 1647, 1677
Bsc4I CCNNNNNNNGG 5 cut(s) 327, 435, 788, 1790, 1871
Bse1I ACTGG 4 cut(s) 55, 1161, 1665, 2161
Bse3DI GCAATG 3 cut(s) 882, 1830, 1842
BseAI TCCGGA 1 cut(s) 320
BseBI CCWGG 2 cut(s) 606, 1052
BseDI CCNNGG 6 cut(s) 234, 355, 1051, 1789, 1902, 2166
BseGI GGATG 5 cut(s) 670, 1294, 1393, 2077, 2118
BseLI CCNNNNNNNGG 5 cut(s) 327, 435, 788, 1790, 1871
BseMI GCAATG 3 cut(s) 882, 1830, 1842
BseMII CTCAG 5 cut(s) 506, 971, 1056, 2232, 2247
BseNI ACTGG 4 cut(s) 55, 1161, 1665, 2161
BseRI GAGGAG 6 cut(s) 83, 86, 89, 584, 1224, 1283
BseYI CCCAGC 1 cut(s) 1439
BsgI GTGCAG 3 cut(s) 858, 1536, 1746
Bsh1236I CGCG 3 cut(s) 114, 138, 867
BshFI GGCC 3 cut(s) 252, 353, 609
BshNI GGYRCC 1 cut(s) 185
BsiHKAI GWGCWC 1 cut(s) 1660
BsiSI CCGG 1 cut(s) 321
BslFI GGGAC 4 cut(s) 411, 1068, 1521, 2027
BslI CCNNNNNNNGG 5 cut(s) 327, 435, 788, 1790, 1871
BsmAI GTCTC 4 cut(s) 510, 1417, 1852, 2121
BsmBI CGTCTC 1 cut(s) 1417
BsmFI GGGAC 4 cut(s) 411, 1068, 1521, 2027
BsmI GAATGC 3 cut(s) 120, 1713, 1720
BsnI GGCC 3 cut(s) 252, 353, 609
Bso31I GGTCTC 1 cut(s) 510
Bsp119I TTCGAA 2 cut(s) 124, 130
Bsp1286I GDGCHC 1 cut(s) 1660
Bsp13I TCCGGA 1 cut(s) 320
Bsp143I GATC 8 cut(s) 415, 554, 672, 841, 862, 960, 1765, 1826
Bsp1720I GCTNAGC 2 cut(s) 1065, 1096
BspACI CCGC 3 cut(s) 112, 194, 1214
BspANI GGCC 3 cut(s) 252, 353, 609
BspCNI CTCAG 5 cut(s) 505, 970, 1057, 2231, 2246
BspEI TCCGGA 1 cut(s) 320
BspFNI CGCG 3 cut(s) 114, 138, 867
BspLI GGNNCC 3 cut(s) 187, 589, 1509
BspMAI CTGCAG 1 cut(s) 154
BspPI GGATC 4 cut(s) 410, 562, 680, 1821
BspQI GCTCTTC 1 cut(s) 1251
BspT104I TTCGAA 2 cut(s) 124, 130
BspT107I GGYRCC 1 cut(s) 185
BspTNI GGTCTC 1 cut(s) 510
BsrDI GCAATG 3 cut(s) 882, 1830, 1842
BsrI ACTGG 4 cut(s) 55, 1161, 1665, 2161
BssECI CCNNGG 6 cut(s) 234, 355, 1051, 1789, 1902, 2166
BssMI GATC 8 cut(s) 415, 554, 672, 841, 862, 960, 1765, 1826
BssNI GRCGYC 1 cut(s) 186
BssT1I CCWWGG 2 cut(s) 234, 2166
Bst2UI CCWGG 2 cut(s) 606, 1052
Bst4CI ACNGT 6 cut(s) 209, 225, 296, 346, 595, 818
Bst6I CTCTTC 8 cut(s) 426, 780, 1251, 1296, 1305, 1347, 1928, 2124
BstACI GRCGYC 1 cut(s) 186
BstAPI GCANNNNNTGC 3 cut(s) 158, 1724, 2183
BstBAI YACGTR 1 cut(s) 774
BstBI TTCGAA 2 cut(s) 124, 130
BstC8I GCNNGC 3 cut(s) 118, 366, 755
BstDEI CTNAG 7 cut(s) 492, 957, 1065, 1096, 1541, 2218, 2233
BstDSI CCRYGG 3 cut(s) 355, 1789, 1902
BstF5I GGATG 5 cut(s) 670, 1294, 1393, 2077, 2118
BstFNI CGCG 3 cut(s) 114, 138, 867
BstH2I RGCGCY 2 cut(s) 189, 1733
BstHHI GCGC 4 cut(s) 116, 188, 1420, 1732
BstKTI GATC 8 cut(s) 418, 557, 675, 844, 865, 963, 1768, 1829
BstMAI GTCTC 4 cut(s) 510, 1417, 1852, 2121
BstMBI GATC 8 cut(s) 415, 554, 672, 841, 862, 960, 1765, 1826
BstNI CCWGG 2 cut(s) 606, 1052
BstNSI RCATGY 1 cut(s) 368
BstSCI CCNGG 2 cut(s) 604, 1050
BstSFI CTRYAG 3 cut(s) 150, 288, 1149
BstSNI TACGTA 1 cut(s) 774
BstUI CGCG 3 cut(s) 114, 138, 867
BstV2I GAAGAC 4 cut(s) 51, 57, 165, 226
BstX2I RGATCY 3 cut(s) 554, 841, 1826
BstXI CCANNNNNNTGG 1 cut(s) 1837
BstYI RGATCY 3 cut(s) 554, 841, 1826
BsuRI GGCC 3 cut(s) 252, 353, 609
BtgI CCRYGG 3 cut(s) 355, 1789, 1902
BtrI CACGTC 1 cut(s) 18
BtsCI GGATG 5 cut(s) 670, 1294, 1393, 2077, 2118
BtsIMutI CAGTG 4 cut(s) 591, 1658, 1697, 2133
Cac8I GCNNGC 3 cut(s) 118, 366, 755
CaiI CAGNNNCTG 2 cut(s) 158, 2225
CfoI GCGC 4 cut(s) 116, 188, 1420, 1732
Cfr13I GGNCC 3 cut(s) 352, 1508, 2004
CseI GACGC 3 cut(s) 127, 1434, 2258
Csp6I GTAC 1 cut(s) 909
CviAII CATG 8 cut(s) 30, 365, 460, 750, 1624, 1693, 1892, 2274
CviQI GTAC 1 cut(s) 909
DdeI CTNAG 7 cut(s) 492, 957, 1065, 1096, 1541, 2218, 2233
DinI GGCGCC 1 cut(s) 187
DpnI GATC 8 cut(s) 417, 556, 674, 843, 864, 962, 1767, 1828
DpnII GATC 8 cut(s) 415, 554, 672, 841, 862, 960, 1765, 1826
DraI TTTAAA 1 cut(s) 922
DriI GACNNNNNGTC 1 cut(s) 2254
EaeI YGGCCR 1 cut(s) 250
Eam1104I CTCTTC 8 cut(s) 426, 780, 1251, 1296, 1305, 1347, 1928, 2124
Eam1105I GACNNNNNGTC 1 cut(s) 2254
EarI CTCTTC 8 cut(s) 426, 780, 1251, 1296, 1305, 1347, 1928, 2124
Eco105I TACGTA 1 cut(s) 774
Eco130I CCWWGG 2 cut(s) 234, 2166
Eco31I GGTCTC 1 cut(s) 510
Eco47I GGWCC 2 cut(s) 1508, 2004
Eco47III AGCGCT 1 cut(s) 1731
Eco57I CTGAAG 6 cut(s) 639, 723, 933, 1371, 1824, 2037
EcoO109I RGGNCCY 1 cut(s) 1508
EcoRI GAATTC 2 cut(s) 126, 132
EcoRII CCWGG 2 cut(s) 604, 1050
EcoT14I CCWWGG 2 cut(s) 234, 2166
EgeI GGCGCC 1 cut(s) 187
EheI GGCGCC 1 cut(s) 187
ErhI CCWWGG 2 cut(s) 234, 2166
Esp3I CGTCTC 1 cut(s) 1417
FaeI CATG 8 cut(s) 33, 368, 463, 753, 1627, 1696, 1895, 2277
FalI AAGNNNNNCTT 2 cut(s) 746, 778
FaqI GGGAC 4 cut(s) 411, 1068, 1521, 2027
FatI CATG 8 cut(s) 29, 364, 459, 749, 1623, 1692, 1891, 2273
FokI GGATG 5 cut(s) 677, 1301, 1400, 2084, 2125
FspBI CTAG 4 cut(s) 812, 1106, 1742, 1824
GlaI GCGC 4 cut(s) 115, 187, 1419, 1731
GsaI CCCAGC 1 cut(s) 1443
GsuI CTGGAG 1 cut(s) 38
HaeII RGCGCY 2 cut(s) 189, 1733
HaeIII GGCC 3 cut(s) 252, 353, 609
HapII CCGG 1 cut(s) 321
HgaI GACGC 3 cut(s) 127, 1434, 2258
HhaI GCGC 4 cut(s) 116, 188, 1420, 1732
Hin1I GRCGYC 1 cut(s) 186
Hin1II CATG 8 cut(s) 33, 368, 463, 753, 1627, 1696, 1895, 2277
Hin6I GCGC 4 cut(s) 114, 186, 1418, 1730
HinP1I GCGC 4 cut(s) 114, 186, 1418, 1730
HincII GTYRAC 1 cut(s) 2259
HindII GTYRAC 1 cut(s) 2259
HindIII AAGCTT 1 cut(s) 1394
HpaII CCGG 1 cut(s) 321
HphI GGTGA 1 cut(s) 1952
Hpy166II GTNNAC 2 cut(s) 803, 2259
Hpy188I TCNGA 7 cut(s) 495, 519, 849, 960, 1468, 1899, 2221
Hpy8I GTNNAC 2 cut(s) 803, 2259
Hpy99I CGWCG 3 cut(s) 22, 25, 28
HpyAV CCTTC 9 cut(s) 318, 620, 698, 1300, 1367, 1429, 1830, 2061, 2062
HpyCH4III ACNGT 6 cut(s) 209, 225, 296, 346, 595, 818
HpyCH4IV ACGT 5 cut(s) 17, 693, 773, 990, 1951
HpyF3I CTNAG 7 cut(s) 492, 957, 1065, 1096, 1541, 2218, 2233
HpySE526I ACGT 5 cut(s) 17, 693, 773, 990, 1951
Hsp92I GRCGYC 1 cut(s) 186
Hsp92II CATG 8 cut(s) 33, 368, 463, 753, 1627, 1696, 1895, 2277
HspAI GCGC 4 cut(s) 114, 186, 1418, 1730
KasI GGCGCC 1 cut(s) 185
Kpn2I TCCGGA 1 cut(s) 320
Kzo9I GATC 8 cut(s) 415, 554, 672, 841, 862, 960, 1765, 1826
LguI GCTCTTC 1 cut(s) 1251
LmnI GCTCC 2 cut(s) 1270, 1655
LweI GCATC 5 cut(s) 247, 1357, 1522, 1669, 2212
MaeI CTAG 4 cut(s) 812, 1106, 1742, 1824
MaeII ACGT 5 cut(s) 17, 693, 773, 990, 1951
MaeIII GTNAC 3 cut(s) 139, 532, 1737
MalI GATC 8 cut(s) 417, 556, 674, 843, 864, 962, 1767, 1828
MboI GATC 8 cut(s) 415, 554, 672, 841, 862, 960, 1765, 1826
MflI RGATCY 3 cut(s) 554, 841, 1826
MhlI GDGCHC 1 cut(s) 1660
MlsI TGGCCA 1 cut(s) 252
MluNI TGGCCA 1 cut(s) 252
Mly113I GGCGCC 1 cut(s) 186
MlyI GAGTC 5 cut(s) 179, 484, 540, 540, 581
MmeI TCCRAC 2 cut(s) 625, 1927
Mox20I TGGCCA 1 cut(s) 252
MroI TCCGGA 1 cut(s) 320
MroXI GAANNNNTTC 1 cut(s) 1809
MscI TGGCCA 1 cut(s) 252
MseI TTAA 7 cut(s) 264, 654, 698, 711, 921, 1182, 1488
MslI CAYNNNNRTG 2 cut(s) 630, 1835
Msp20I TGGCCA 1 cut(s) 252
MspA1I CMGCKG 3 cut(s) 158, 875, 2237
MspI CCGG 1 cut(s) 321
MspR9I CCNGG 2 cut(s) 606, 1052
Mva1269I GAATGC 3 cut(s) 120, 1713, 1720
MvaI CCWGG 2 cut(s) 606, 1052
MvnI CGCG 3 cut(s) 114, 138, 867
NarI GGCGCC 1 cut(s) 186
NdeII GATC 8 cut(s) 415, 554, 672, 841, 862, 960, 1765, 1826
NlaIII CATG 8 cut(s) 33, 368, 463, 753, 1627, 1696, 1895, 2277
NlaIV GGNNCC 3 cut(s) 187, 589, 1509
NmuCI GTSAC 2 cut(s) 139, 532
NspI RCATGY 1 cut(s) 368
NspV TTCGAA 2 cut(s) 124, 130
PaeI GCATGC 1 cut(s) 368
PciSI GCTCTTC 1 cut(s) 1251
PctI GAATGC 3 cut(s) 120, 1713, 1720
PdmI GAANNNNTTC 1 cut(s) 1809
PfeI GAWTC 5 cut(s) 470, 520, 1247, 1796, 2077
PleI GAGTC 5 cut(s) 178, 484, 539, 540, 580
PluTI GGCGCC 1 cut(s) 189
PpsI GAGTC 5 cut(s) 178, 484, 539, 540, 580
Ppu21I YACGTR 1 cut(s) 774
PpuMI RGGWCCY 1 cut(s) 1508
PshBI ATTAAT 2 cut(s) 654, 1182
PsiI TTATAA 1 cut(s) 998
Psp5II RGGWCCY 1 cut(s) 1508
Psp6I CCWGG 2 cut(s) 604, 1050
PspFI CCCAGC 1 cut(s) 1439
PspGI CCWGG 2 cut(s) 604, 1050
PspN4I GGNNCC 3 cut(s) 187, 589, 1509
PspPI GGNCC 3 cut(s) 352, 1508, 2004
PspPPI RGGWCCY 1 cut(s) 1508
PsrI GAACNNNNNNTAC 2 cut(s) 1146, 1178
PstI CTGCAG 1 cut(s) 154
PstNI CAGNNNCTG 2 cut(s) 158, 2225
PsuI RGATCY 3 cut(s) 554, 841, 1826
PvuII CAGCTG 3 cut(s) 158, 875, 2237
RsaI GTAC 1 cut(s) 910
RsaNI GTAC 1 cut(s) 909
RseI CAYNNNNRTG 2 cut(s) 630, 1835
SapI GCTCTTC 1 cut(s) 1251
SaqAI TTAA 7 cut(s) 264, 654, 698, 711, 921, 1182, 1488
Sau3AI GATC 8 cut(s) 415, 554, 672, 841, 862, 960, 1765, 1826
Sau96I GGNCC 3 cut(s) 352, 1508, 2004
ScaI AGTACT 1 cut(s) 910
SchI GAGTC 5 cut(s) 179, 484, 540, 540, 581
ScrFI CCNGG 2 cut(s) 606, 1052
SduI GDGCHC 1 cut(s) 1660
SfaNI GCATC 5 cut(s) 247, 1357, 1522, 1669, 2212
SfcI CTRYAG 3 cut(s) 150, 288, 1149
SfoI GGCGCC 1 cut(s) 187
SfuI TTCGAA 2 cut(s) 124, 130
SinI GGWCC 2 cut(s) 1508, 2004
SmiMI CAYNNNNRTG 2 cut(s) 630, 1835
SmlI CTYRAG 7 cut(s) 832, 1013, 1587, 1733, 1853, 2081, 2187
SmoI CTYRAG 7 cut(s) 832, 1013, 1587, 1733, 1853, 2081, 2187
SnaBI TACGTA 1 cut(s) 774
SphI GCATGC 1 cut(s) 368
SsiI CCGC 3 cut(s) 112, 194, 1214
SspDI GGCGCC 1 cut(s) 185
SspI AATATT 1 cut(s) 715
SspMI CTAG 4 cut(s) 812, 1106, 1742, 1824
StyD4I CCNGG 2 cut(s) 604, 1050
StyI CCWWGG 2 cut(s) 234, 2166
TaaI ACNGT 6 cut(s) 209, 225, 296, 346, 595, 818
TaiI ACGT 5 cut(s) 20, 696, 776, 993, 1954
TaqI TCGA 4 cut(s) 124, 130, 1480, 2130
TatI WGTACW 1 cut(s) 908
TauI GCSGC 2 cut(s) 114, 197
TfiI GAWTC 5 cut(s) 470, 520, 1247, 1796, 2077
Tru1I TTAA 7 cut(s) 264, 654, 698, 711, 921, 1182, 1488
Tru9I TTAA 7 cut(s) 264, 654, 698, 711, 921, 1182, 1488
TscAI CASTG 4 cut(s) 598, 1665, 1704, 2140
TseFI GTSAC 2 cut(s) 139, 532
Tsp45I GTSAC 2 cut(s) 139, 532
TspDTI ATGAA 4 cut(s) 663, 1314, 1332, 1341
TspGWI ACGGA 4 cut(s) 231, 565, 1660, 1778
TspRI CASTG 4 cut(s) 598, 1665, 1704, 2140
VpaK11BI GGWCC 2 cut(s) 1508, 2004
VspI ATTAAT 2 cut(s) 654, 1182
XapI RAATTY 6 cut(s) 126, 132, 464, 893, 944, 2144
XbaI TCTAGA 2 cut(s) 1105, 1823
XceI RCATGY 1 cut(s) 368
XcmI CCANNNNNNNNNTGG 1 cut(s) 346
XmnI GAANNNNTTC 1 cut(s) 1809
XspI CTAG 4 cut(s) 812, 1106, 1742, 1824
ZrmI AGTACT 1 cut(s) 910
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.