Rroxscaffold_1G00029290

Involved in chlorophyll biosynthesis. Catalyzes the insertion of magnesium ion into protoporphyrin IX to yield Mg- protoporphyrin IX

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0005823
Physical Location & Seq
Forward (+)
37841810 .. 37855664
13855 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00029290.1

Sequence Viewer

Length: 2328 bp
ATGCAGTTTCAACAAGTATCCTATTTGGTCTTAGAAAAGGAAAGATGGAGGGATTCTTATTGGTGGGGGCACTTGCATAATTTTGAAAGGAGTGGGTTTCACATTTCTTCCAATCAAGATTTTCAACAAGGAGAGAGTGTTTTGATTAGGAGAAGTGGAAGGTCAGAAGTCGTAGTAGACAATGCCATCACTGAACTTGGCCATGCAGCTGCCGTTGTTGAGTCTTCAAACGGCGCCGTAGCGGCGAAACAGGACAATTCCTACGGAAGACAGTTCTTTCCCTTGGCTGCCGTTGTTGGCCAAGATGCTATTAAAACTGCTCTGTTGCTTGGGGCTATAGACCGTGAAATTGGAGGAATTGCTATCTCCGGAAGGAGGGGAACAGCCAAAACAGTAATGGCCCGTGGTTTGCATGCAATTTTACCACCCATTGAAGTAGTTGTTGGTTCAATAGCAAATGCTGATCCAGCCTGTCCCGAAGAGTGGGAAGATGTTTTACCTGAATGCATGGAATTTGATTCTGCTGGTAATGTCAAGACTCAGATTGTTAGGTCTCCTTTTGTTCAGATTCCACTTGGAATCACAGAGGACAGACTCATTGGATCTGTTGATGTTGAGGAGTCCGTGAGAACAGGAACCACTGTTTTCCAACCTGGCCTTCTTGCTGAAGCTCATAGAGGTGTATTATATGTTGATGAAATTAATCTTTTGGATGAGGGGATCAGTAATTTGCTTCTCAACGTATTAACTGAAGGAGTTAATATTGTTGAGAGAGAGGGAATGAGCTTTAGACACCCATGCAAGCCCCTCTTGATTGCTACGTATAATCCAGAAGAGGGTGCTGTGCGTGAACACTTACTAGACCGTATTGCAATCAACTTGAGTGCAGATCTTCCGATGAGTTTTGATGATCGTGTTGCAGCTGTGGGCATTGCGACAAAATTTCAGGAATATAGTACTGAAGTCTTTAAAATGGTTCAAGAAGAAACAGAATTTGCAAAAACTCAGATCATTTTGGCAAGAGAGTATCTAAAGGACGTAGTTATAAGCAGAGAACAACTCAAGTATCTGGTTTTGGAAGCCCTGCGAGGTGGTTGCCAGGGACATAGAGCTGAGCTATATGCTTCCCGTGTTGCAAAATGCTTAGCTGCTCTAGATGGACGAGAAAAAGTTTATGCTGATGACCTGAAAAAAGCTGTAGAACTGGTCATACTTCCTCGCTCAATCGTTAATGAGAACCCACCAGAACAGCAAAATCAACCGCCTCCACCTCCACCACCTCCTCAAAATCAAGAATCTGGGGAAGAGCAAAATGAAGAGCAAGAAGAAAAAGAGGATGAAAATGATGAAGAGAAGGAAGAGAATGAAGATAATGAAGAGCAGGAACAGCTACCTGAAGAGTTTGTCTTTGATGCAGAAGGGGGTTTGGTGGATGAGAAGCTTCTTTTTTTGCACAACAAGCGCAGAGACGCAAAGGGAAGGCTGGGAGAGCAAAGAATGTTATCTTTTCAGAGGATAGAGGTCGATACATTAAGCCAATGCTTCCAAAGTCATTTGGGACCTGTAAAGAGATTAGCTGTTGATGCAACTCTTAGAGTTGCTGCACCATATCAAAAATTGCGTAGGGAGAAGAACACTCAAGTCGGTAGGAAAGTTTTTGTTGAAAAAACTGACATGAGAGCAAAGAGAATGGCACGGAAAGCAGGAGCACTGGTTATATTTGTTGTTGATGCAAGTGGAAGCATGGCACTGAACCGAATGCAGAATGCAAAAGGTGCAGCGCTAAAGTTACTAGCCGAAAGTTATACGAGCAGAGATCAGGTTTCTATTATTCCTTTCCGTGGAGATTCTGCTGAAGTTCTTCTGCCTCCTTCTAGATCCATTGCAATGGCAAGAAATCGTCTTGAGAGACTTCCCTGTGGTGGAGGTTCTCCCCTTGCTCATGGTCTGACCACGGCTGTAAGGGTTGGACTAAATGCAGAGAAGAGTGGTGATGTTGGACGTATAATGATTGTTGCAATAACTGATGGTAGAGCCAATATATCACTAAAAAGGTCCAATGACCCTGAAGCTGCTGCTGCTGCTACTGATGTCCCAAGACCTTCGGCGAAGGAGTTGAAGGATGAGATTCTTGAGGTTGCTGGTAAAATATACAAAGCAGGGATGTCTCTTCTCGTCATCGACACTGAAAATAAATTTGTTTCAACTGGTTTTGCCAAGGAGATTGCAAGAGTTGCTCAAGGAAAATACTATTACTTGCCAAATGCCTCAGATGCTGTAATCTCAGCTGCCACAAAGGACGCTTTGTCAACATTGAAAAACACATGA

Protein Analysis

775

Amino Acids

85.43

Weight (kDa)

5.27

Isoelectric Point (pI)

47.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mg_chelatase PF01078 212 - 279 9.3e-07 Magnesium chelatase, subunit ChlI
AAA_lid_2 PF17863 359 - 419 1.4e-13 AAA lid domain
VWA_2 PF13519 572 - 677 1.7e-12 von Willebrand factor type A domain
VWA PF00092 572 - 693 4.3e-06 von Willebrand factor type A domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1046
AccB1I GGYRCC 1 cut(s) 233
AccI GTMKAC 1 cut(s) 177
AccIII TCCGGA 1 cut(s) 368
AciI CCGC 2 cut(s) 242, 1262
AclWI GGATC 4 cut(s) 458, 610, 728, 1872
AcoI YGGCCR 2 cut(s) 199, 298
AcsI RAATTY 4 cut(s) 512, 941, 992, 2195
AcuI CTGAAG 6 cut(s) 687, 771, 981, 1416, 1875, 2088
AcyI GRCGYC 1 cut(s) 234
AfaI GTAC 1 cut(s) 958
AfeI AGCGCT 1 cut(s) 1782
AfiI CCNNNNNNNGG 5 cut(s) 375, 483, 836, 1841, 1922
AhdI GACNNNNNGTC 1 cut(s) 2305
AjnI CCWGG 2 cut(s) 652, 1098
AjuI GAANNNNNNNTTGG 4 cut(s) 426, 458, 642, 674
Alw21I GWGCWC 1 cut(s) 1711
Alw26I GTCTC 4 cut(s) 558, 1461, 1903, 2172
AlwI GGATC 4 cut(s) 458, 610, 728, 1872
AlwNI CAGNNNCTG 1 cut(s) 2276
Aor13HI TCCGGA 1 cut(s) 368
Aor51HI AGCGCT 1 cut(s) 1782
AoxI GGCC 4 cut(s) 199, 298, 399, 655
ApoI RAATTY 4 cut(s) 512, 941, 992, 2195
AseI ATTAAT 1 cut(s) 702
Asp700I GAANNNNTTC 1 cut(s) 1860
AspLEI GCGC 3 cut(s) 236, 1464, 1783
AspS9I GGNCC 3 cut(s) 400, 1559, 2055
AsuHPI GGTGA 1 cut(s) 2003
AvaII GGWCC 2 cut(s) 1559, 2055
BaeGI GKGCMC 1 cut(s) 72
BaeI ACNNNNGTAYC 2 cut(s) 1049, 1082
BalI TGGCCA 2 cut(s) 201, 300
BanI GGYRCC 1 cut(s) 233
BarI GAAGNNNNNNTAC 2 cut(s) 480, 512
BbsI GAAGAC 2 cut(s) 216, 274
Bbv12I GWGCWC 1 cut(s) 1711
BccI CCATC 4 cut(s) 39, 194, 1151, 2021
BceAI ACGGC 5 cut(s) 197, 221, 247, 275, 1971
BcgI CGANNNNNNTGC 2 cut(s) 1077, 1111
BciT130I CCWGG 2 cut(s) 654, 1100
BciVI GTATCC 1 cut(s) 28
BcoDI GTCTC 4 cut(s) 558, 1461, 1903, 2172
BfaI CTAG 4 cut(s) 860, 1154, 1793, 1875
BfmI CTRYAG 2 cut(s) 336, 1197
BfoI RGCGCY 2 cut(s) 237, 1784
BfuI GTATCC 1 cut(s) 28
BglI GCCNNNNNGGC 1 cut(s) 242
BglII AGATCT 1 cut(s) 889
BlpI GCTNAGC 2 cut(s) 1113, 1144
BmcAI AGTACT 1 cut(s) 958
Bme1390I CCNGG 2 cut(s) 654, 1100
Bme18I GGWCC 2 cut(s) 1559, 2055
BmeRI GACNNNNNGTC 1 cut(s) 2305
BmgT120I GGNCC 3 cut(s) 400, 1559, 2055
BmiI GGNNCC 3 cut(s) 235, 637, 1560
BmrFI CCNGG 2 cut(s) 654, 1100
BmsI GCATC 5 cut(s) 295, 1402, 1573, 1720, 2263
BpiI GAAGAC 2 cut(s) 216, 274
BplI GAGNNNNNCTC 2 cut(s) 1044, 1076
Bpu1102I GCTNAGC 2 cut(s) 1113, 1144
BpuEI CTTGAG 6 cut(s) 901, 1046, 1623, 1925, 2153, 2223
BsaAI YACGTR 1 cut(s) 822
BsaHI GRCGYC 1 cut(s) 234
BsaI GGTCTC 1 cut(s) 558
BsaJI CCNNGG 6 cut(s) 282, 403, 1099, 1840, 1953, 2217
BsaWI WCCGGW 1 cut(s) 368
BsaXI ACNNNNNCTCC 4 cut(s) 123, 153, 1698, 1728
Bsc4I CCNNNNNNNGG 5 cut(s) 375, 483, 836, 1841, 1922
Bse1I ACTGG 3 cut(s) 1209, 1716, 2212
Bse3DI GCAATG 3 cut(s) 930, 1881, 1893
BseAI TCCGGA 1 cut(s) 368
BseBI CCWGG 2 cut(s) 654, 1100
BseDI CCNNGG 6 cut(s) 282, 403, 1099, 1840, 1953, 2217
BseGI GGATG 5 cut(s) 718, 1342, 1438, 2128, 2169
BseLI CCNNNNNNNGG 5 cut(s) 375, 483, 836, 1841, 1922
BseMI GCAATG 3 cut(s) 930, 1881, 1893
BseMII CTCAG 5 cut(s) 554, 1019, 1104, 2283, 2298
BseNI ACTGG 3 cut(s) 1209, 1716, 2212
BseRI GAGGAG 2 cut(s) 632, 1272
BseSI GKGCMC 1 cut(s) 72
BseYI CCCAGC 1 cut(s) 1483
BsgI GTGCAG 3 cut(s) 906, 1587, 1797
BshFI GGCC 4 cut(s) 201, 300, 401, 657
BshNI GGYRCC 1 cut(s) 233
BsiHKAI GWGCWC 1 cut(s) 1711
BsiSI CCGG 1 cut(s) 369
BslFI GGGAC 4 cut(s) 459, 1116, 1572, 2078
BslI CCNNNNNNNGG 5 cut(s) 375, 483, 836, 1841, 1922
BsmAI GTCTC 4 cut(s) 558, 1461, 1903, 2172
BsmBI CGTCTC 1 cut(s) 1461
BsmFI GGGAC 4 cut(s) 459, 1116, 1572, 2078
BsmI GAATGC 3 cut(s) 509, 1764, 1771
BsnI GGCC 4 cut(s) 201, 300, 401, 657
Bso31I GGTCTC 1 cut(s) 558
Bsp1286I GDGCHC 2 cut(s) 72, 1711
Bsp13I TCCGGA 1 cut(s) 368
Bsp143I GATC 8 cut(s) 463, 602, 720, 889, 910, 1008, 1816, 1877
Bsp1720I GCTNAGC 2 cut(s) 1113, 1144
BspACI CCGC 2 cut(s) 242, 1262
BspANI GGCC 4 cut(s) 201, 300, 401, 657
BspCNI CTCAG 5 cut(s) 553, 1018, 1105, 2282, 2297
BspEI TCCGGA 1 cut(s) 368
BspLI GGNNCC 3 cut(s) 235, 637, 1560
BspPI GGATC 4 cut(s) 458, 610, 728, 1872
BspQI GCTCTTC 3 cut(s) 1299, 1311, 1371
BspT107I GGYRCC 1 cut(s) 233
BspTNI GGTCTC 1 cut(s) 558
BsrDI GCAATG 3 cut(s) 930, 1881, 1893
BsrI ACTGG 3 cut(s) 1209, 1716, 2212
BssECI CCNNGG 6 cut(s) 282, 403, 1099, 1840, 1953, 2217
BssMI GATC 8 cut(s) 463, 602, 720, 889, 910, 1008, 1816, 1877
BssNI GRCGYC 1 cut(s) 234
BssT1I CCWWGG 2 cut(s) 282, 2217
Bst2UI CCWGG 2 cut(s) 654, 1100
Bst4CI ACNGT 5 cut(s) 273, 344, 394, 643, 866
BstACI GRCGYC 1 cut(s) 234
BstAPI GCANNNNNTGC 2 cut(s) 1775, 2234
BstBAI YACGTR 1 cut(s) 822
BstC8I GCNNGC 2 cut(s) 414, 803
BstDEI CTNAG 8 cut(s) 31, 540, 1005, 1113, 1144, 1592, 2269, 2284
BstDSI CCRYGG 3 cut(s) 403, 1840, 1953
BstF5I GGATG 5 cut(s) 718, 1342, 1438, 2128, 2169
BstH2I RGCGCY 2 cut(s) 237, 1784
BstHHI GCGC 3 cut(s) 236, 1464, 1783
BstKTI GATC 8 cut(s) 466, 605, 723, 892, 913, 1011, 1819, 1880
BstMAI GTCTC 4 cut(s) 558, 1461, 1903, 2172
BstMBI GATC 8 cut(s) 463, 602, 720, 889, 910, 1008, 1816, 1877
BstNI CCWGG 2 cut(s) 654, 1100
BstNSI RCATGY 1 cut(s) 416
BstSCI CCNGG 2 cut(s) 652, 1098
BstSFI CTRYAG 2 cut(s) 336, 1197
BstSLI GKGCMC 1 cut(s) 72
BstSNI TACGTA 1 cut(s) 822
BstV2I GAAGAC 2 cut(s) 216, 274
BstX2I RGATCY 3 cut(s) 602, 889, 1877
BstXI CCANNNNNNTGG 1 cut(s) 1888
BstYI RGATCY 3 cut(s) 602, 889, 1877
BsuI GTATCC 1 cut(s) 28
BsuRI GGCC 4 cut(s) 201, 300, 401, 657
BtgI CCRYGG 3 cut(s) 403, 1840, 1953
BtsCI GGATG 5 cut(s) 718, 1342, 1438, 2128, 2169
BtsIMutI CAGTG 5 cut(s) 189, 639, 1709, 1748, 2184
Cac8I GCNNGC 2 cut(s) 414, 803
CaiI CAGNNNCTG 1 cut(s) 2276
CfoI GCGC 3 cut(s) 236, 1464, 1783
Cfr13I GGNCC 3 cut(s) 400, 1559, 2055
CseI GACGC 2 cut(s) 1478, 2309
Csp6I GTAC 1 cut(s) 957
CviAII CATG 8 cut(s) 203, 413, 508, 798, 1675, 1744, 1943, 2325
CviQI GTAC 1 cut(s) 957
DdeI CTNAG 8 cut(s) 31, 540, 1005, 1113, 1144, 1592, 2269, 2284
DinI GGCGCC 1 cut(s) 235
DpnI GATC 8 cut(s) 465, 604, 722, 891, 912, 1010, 1818, 1879
DpnII GATC 8 cut(s) 463, 602, 720, 889, 910, 1008, 1816, 1877
DraI TTTAAA 1 cut(s) 970
DriI GACNNNNNGTC 1 cut(s) 2305
EaeI YGGCCR 2 cut(s) 199, 298
Eam1105I GACNNNNNGTC 1 cut(s) 2305
Eco105I TACGTA 1 cut(s) 822
Eco130I CCWWGG 2 cut(s) 282, 2217
Eco31I GGTCTC 1 cut(s) 558
Eco47I GGWCC 2 cut(s) 1559, 2055
Eco47III AGCGCT 1 cut(s) 1782
Eco57I CTGAAG 6 cut(s) 687, 771, 981, 1416, 1875, 2088
EcoO109I RGGNCCY 1 cut(s) 1559
EcoRII CCWGG 2 cut(s) 652, 1098
EcoT14I CCWWGG 2 cut(s) 282, 2217
EcoT22I ATGCAT 1 cut(s) 509
EgeI GGCGCC 1 cut(s) 235
EheI GGCGCC 1 cut(s) 235
ErhI CCWWGG 2 cut(s) 282, 2217
Esp3I CGTCTC 1 cut(s) 1461
FaeI CATG 8 cut(s) 206, 416, 511, 801, 1678, 1747, 1946, 2328
FalI AAGNNNNNCTT 2 cut(s) 794, 826
FaqI GGGAC 4 cut(s) 459, 1116, 1572, 2078
FatI CATG 8 cut(s) 202, 412, 507, 797, 1674, 1743, 1942, 2324
FblI GTMKAC 1 cut(s) 177
FokI GGATG 5 cut(s) 725, 1349, 1445, 2135, 2176
FspBI CTAG 4 cut(s) 860, 1154, 1793, 1875
GlaI GCGC 3 cut(s) 235, 1463, 1782
GsaI CCCAGC 1 cut(s) 1487
HaeII RGCGCY 2 cut(s) 237, 1784
HaeIII GGCC 4 cut(s) 201, 300, 401, 657
HapII CCGG 1 cut(s) 369
HgaI GACGC 2 cut(s) 1478, 2309
HhaI GCGC 3 cut(s) 236, 1464, 1783
Hin1I GRCGYC 1 cut(s) 234
Hin1II CATG 8 cut(s) 206, 416, 511, 801, 1678, 1747, 1946, 2328
Hin6I GCGC 3 cut(s) 234, 1462, 1781
HinP1I GCGC 3 cut(s) 234, 1462, 1781
HincII GTYRAC 1 cut(s) 2310
HindII GTYRAC 1 cut(s) 2310
HindIII AAGCTT 1 cut(s) 1439
HpaII CCGG 1 cut(s) 369
HphI GGTGA 1 cut(s) 2003
Hpy166II GTNNAC 3 cut(s) 178, 851, 2310
Hpy188I TCNGA 8 cut(s) 166, 543, 567, 897, 1008, 1512, 1950, 2272
Hpy8I GTNNAC 3 cut(s) 178, 851, 2310
HpyCH4III ACNGT 5 cut(s) 273, 344, 394, 643, 866
HpyCH4IV ACGT 4 cut(s) 741, 821, 1038, 2002
HpyF3I CTNAG 8 cut(s) 31, 540, 1005, 1113, 1144, 1592, 2269, 2284
HpySE526I ACGT 4 cut(s) 741, 821, 1038, 2002
Hsp92I GRCGYC 1 cut(s) 234
Hsp92II CATG 8 cut(s) 206, 416, 511, 801, 1678, 1747, 1946, 2328
HspAI GCGC 3 cut(s) 234, 1462, 1781
KasI GGCGCC 1 cut(s) 233
Kpn2I TCCGGA 1 cut(s) 368
Kzo9I GATC 8 cut(s) 463, 602, 720, 889, 910, 1008, 1816, 1877
LguI GCTCTTC 3 cut(s) 1299, 1311, 1371
LmnI GCTCC 1 cut(s) 1706
LweI GCATC 5 cut(s) 295, 1402, 1573, 1720, 2263
MaeI CTAG 4 cut(s) 860, 1154, 1793, 1875
MaeII ACGT 4 cut(s) 741, 821, 1038, 2002
MaeIII GTNAC 1 cut(s) 1788
MalI GATC 8 cut(s) 465, 604, 722, 891, 912, 1010, 1818, 1879
MboI GATC 8 cut(s) 463, 602, 720, 889, 910, 1008, 1816, 1877
MflI RGATCY 3 cut(s) 602, 889, 1877
MhlI GDGCHC 2 cut(s) 72, 1711
MlsI TGGCCA 2 cut(s) 201, 300
MluNI TGGCCA 2 cut(s) 201, 300
Mly113I GGCGCC 1 cut(s) 234
MlyI GAGTC 4 cut(s) 230, 532, 588, 629
MmeI TCCRAC 3 cut(s) 673, 1948, 1978
Mox20I TGGCCA 2 cut(s) 201, 300
Mph1103I ATGCAT 1 cut(s) 509
MroI TCCGGA 1 cut(s) 368
MroXI GAANNNNTTC 1 cut(s) 1860
MscI TGGCCA 2 cut(s) 201, 300
MseI TTAA 7 cut(s) 312, 702, 746, 759, 969, 1230, 1532
MslI CAYNNNNRTG 2 cut(s) 678, 1886
Msp20I TGGCCA 2 cut(s) 201, 300
MspA1I CMGCKG 3 cut(s) 209, 923, 2288
MspI CCGG 1 cut(s) 369
MspR9I CCNGG 2 cut(s) 654, 1100
Mva1269I GAATGC 3 cut(s) 509, 1764, 1771
MvaI CCWGG 2 cut(s) 654, 1100
NarI GGCGCC 1 cut(s) 234
NdeII GATC 8 cut(s) 463, 602, 720, 889, 910, 1008, 1816, 1877
NlaIII CATG 8 cut(s) 206, 416, 511, 801, 1678, 1747, 1946, 2328
NlaIV GGNNCC 3 cut(s) 235, 637, 1560
NsiI ATGCAT 1 cut(s) 509
NspI RCATGY 1 cut(s) 416
PaeI GCATGC 1 cut(s) 416
PciSI GCTCTTC 3 cut(s) 1299, 1311, 1371
PctI GAATGC 3 cut(s) 509, 1764, 1771
PdmI GAANNNNTTC 1 cut(s) 1860
PfeI GAWTC 7 cut(s) 53, 518, 568, 579, 1295, 1847, 2128
PleI GAGTC 4 cut(s) 229, 532, 588, 628
PluTI GGCGCC 1 cut(s) 237
PpsI GAGTC 4 cut(s) 229, 532, 588, 628
Ppu21I YACGTR 1 cut(s) 822
PpuMI RGGWCCY 1 cut(s) 1559
PshBI ATTAAT 1 cut(s) 702
PsiI TTATAA 1 cut(s) 1046
Psp5II RGGWCCY 1 cut(s) 1559
Psp6I CCWGG 2 cut(s) 652, 1098
PspFI CCCAGC 1 cut(s) 1483
PspGI CCWGG 2 cut(s) 652, 1098
PspN4I GGNNCC 3 cut(s) 235, 637, 1560
PspPI GGNCC 3 cut(s) 400, 1559, 2055
PspPPI RGGWCCY 1 cut(s) 1559
PsrI GAACNNNNNNTAC 2 cut(s) 1194, 1226
PstNI CAGNNNCTG 1 cut(s) 2276
PsuI RGATCY 3 cut(s) 602, 889, 1877
PvuII CAGCTG 3 cut(s) 209, 923, 2288
RsaI GTAC 1 cut(s) 958
RsaNI GTAC 1 cut(s) 957
RseI CAYNNNNRTG 2 cut(s) 678, 1886
SapI GCTCTTC 3 cut(s) 1299, 1311, 1371
SaqAI TTAA 7 cut(s) 312, 702, 746, 759, 969, 1230, 1532
Sau3AI GATC 8 cut(s) 463, 602, 720, 889, 910, 1008, 1816, 1877
Sau96I GGNCC 3 cut(s) 400, 1559, 2055
ScaI AGTACT 1 cut(s) 958
SchI GAGTC 4 cut(s) 230, 532, 588, 629
ScrFI CCNGG 2 cut(s) 654, 1100
SduI GDGCHC 2 cut(s) 72, 1711
SfaNI GCATC 5 cut(s) 295, 1402, 1573, 1720, 2263
SfcI CTRYAG 2 cut(s) 336, 1197
SfoI GGCGCC 1 cut(s) 235
SinI GGWCC 2 cut(s) 1559, 2055
SmiMI CAYNNNNRTG 2 cut(s) 678, 1886
SmlI CTYRAG 6 cut(s) 880, 1061, 1638, 1904, 2132, 2238
SmoI CTYRAG 6 cut(s) 880, 1061, 1638, 1904, 2132, 2238
SnaBI TACGTA 1 cut(s) 822
SphI GCATGC 1 cut(s) 416
SsiI CCGC 2 cut(s) 242, 1262
SspDI GGCGCC 1 cut(s) 233
SspI AATATT 1 cut(s) 763
SspMI CTAG 4 cut(s) 860, 1154, 1793, 1875
StyD4I CCNGG 2 cut(s) 652, 1098
StyI CCWWGG 2 cut(s) 282, 2217
TaaI ACNGT 5 cut(s) 273, 344, 394, 643, 866
TaiI ACGT 4 cut(s) 744, 824, 1041, 2005
TaqI TCGA 2 cut(s) 1524, 2181
TatI WGTACW 1 cut(s) 956
TauI GCSGC 1 cut(s) 245
TfiI GAWTC 7 cut(s) 53, 518, 568, 579, 1295, 1847, 2128
Tru1I TTAA 7 cut(s) 312, 702, 746, 759, 969, 1230, 1532
Tru9I TTAA 7 cut(s) 312, 702, 746, 759, 969, 1230, 1532
TscAI CASTG 5 cut(s) 196, 646, 1716, 1755, 2191
TspDTI ATGAA 6 cut(s) 711, 1329, 1353, 1362, 1380, 1389
TspGWI ACGGA 4 cut(s) 279, 613, 1711, 1829
TspRI CASTG 5 cut(s) 196, 646, 1716, 1755, 2191
VpaK11BI GGWCC 2 cut(s) 1559, 2055
VspI ATTAAT 1 cut(s) 702
XapI RAATTY 4 cut(s) 512, 941, 992, 2195
XbaI TCTAGA 2 cut(s) 1153, 1874
XceI RCATGY 1 cut(s) 416
XcmI CCANNNNNNNNNTGG 1 cut(s) 394
XmiI GTMKAC 1 cut(s) 177
XmnI GAANNNNTTC 1 cut(s) 1860
XspI CTAG 4 cut(s) 860, 1154, 1793, 1875
ZrmI AGTACT 1 cut(s) 958
Zsp2I ATGCAT 1 cut(s) 509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.