AT1G09010

Glycoside hydrolase family 2 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0006337
Physical Location & Seq
Reverse (-)
2895166 .. 2899446
4281 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G09010.1

Sequence Viewer

Length: 2835 bp
ATGGCGGAGATCGGGAAGACGGTGTTAGATTTCGGCTGGATAGCGGCGAGATCCACGGAGGTCGATGTCAACGGCGTTCAGCTCACTACCACCAATCCTCCGGCCATCAGTTCCGAATCTCGGTGGATGGAAGCCGCCGTACCTGGAACTGTATTGGGCACATTGGTGAAAAACAAAGCAATTCCGGATCCTTTTTATGGACTTGAAAATGAAGCGATCACAGATATTGCCGATTCCGGAAGGGACTACTACACTTTCTGGTTCTTCACTAAATTCCAATGTCAAAGGTTATTGAATCAATACGTGCATTTGAATTTCCGTGCCATCAACTACTCTGCACAAGTGTTTGTAAATGGCCACAAGACAGAGCTTCCCAAAGGGATGTTTCGCAGACATACACTTGACGTCACTGATATTCTCCATCCTGAGAGCAATTTACTTGCTCTTATTGTTCATCCCCCAGATCATCCTGGGACTATACCTCCCGAGGGAGGCCAAGGTGGTGATCATGAGATTGGAAAAGATGTAGCTGCACAGTATGTGCAAGGTTGGGACTGGATTTGTCCAATAAGGGACCGGAATACTGGCATATGGGATGAAGTGTCAATATCTGTCACCGGGCCTGTAAGAATAATTGATCCCCATTTGGTCTCAACCTTCTTTGACGATTACAAGAGAGCTTACTTGCATGTGACTGCTGAACTGGAAAACAAAAGCACATGGAATACTGAATGTTCTGTGAATATTCAAATAACAGCCGAACTCGAAAACGGTGTCTGTTTAGTAGAGCATCTTCAGACAGAGAATGTTTTGATCCCTGCTCAAGGACGTATTCAGCACACATTTAAACCACTCTATTTCTATAAACCTGAATTGTGGTGGCCCAACGGGATGGGAAAGCAAAACCTTTATGACATCTTGATCACTGTTGTTGTGAATGAATTCGGAGAATCTGATTCGTGGATGCAGCCATTTGGATTCCGAAAGATTGAGAGTGTTATTGATAGTGTTACTGGTGGAAGGCTGTTCAAGATCAACGGAGAGCCGATCTTCATCCGTGGTGGCAATTGGATACTATCAGATGGACTGTTACGTCTCTCTAAAGAACGTTACAGAACCGACATAAAATTCCATGCAGATATGAACATGAACATGATCCGTTGCTGGGGCGGTGGGTTGGCTGAAAGGCCAGAGTTTTATCACTTTTGTGATATCTACGGCTTGTTGGTGTGGCAAGAGTTTTGGATCACTGGCGATGTTGATGGAAGAGGTGTTCCAGTATCAAATCCGAATGGACCACTAGACCATGAATTATTCCTCTTATGTGCTCGTGATACTGTCAAACTTCTGAGAAACCATCCAAGCCTGGCTCTTTGGGTGGGTGGAAATGAACAAGTTCCGCCAAAAGACATAAATGAGGCTCTAAAGCAAGACCTTAGGCTTCATTCTTATTTTGAAACCCAATTGTTATCAGACAAAGATTCAGATCCTAGTGTTTATCTTGACGGTACTAGAGTTTATATTCAAGGATCTATGTGGGACGGCTTTGCAGATGGAAAGGGGAACTTCACTGACGGCCCTTATGAGATTCAATACCCTGAAGATTTTTTTAAAGACACATATTACAAGTATGGGTTCAATCCGGAGGTCGGCTCGGTTGGAATGCCAGTTGCAGAGACGATAAGAGCGACAATGCCTCCAGAAGGTTGGACGATTCCTCTGTTCAAAAAGGGTTTGGACGGGTTTATTAAAGAAGTGCCAAATCGAATGTGGGATTATCACAAGTACATTCCATATTCTAATCCTGGAAAAGTGCATGACCAGATTCTGATGTACGGTACTCCAGAGAATCTCGATGACTTCTGCTTGAAGGCTCAACTGGTAAACTACATTCAGTACAGAGCTCTATTCGAGGGTTGGAGTTCGCAAATGTGGACGAAGTACACGGGTGTCTTGATCTGGAAGAACCAAAATCCATGGACTGGTCTCAGAGGTCAATTCTACGATCATCTTCTCGACCAAACAGCCAGTTTCTATGGCTGCCGTTCCGCTGCAGAGCCAGTCCATGTACAATTGAACCTGGCAAGTTACTTTGTTGAGGTTGTAAACACGACTTCTAAAGAGCTGTCGGATGTGGCAATAGAGGCATCAGTATGGGACCTAGACGGGAACTGCCCATACTATAAAGTGTTTAAAATTGTCTCTGCCCCACCAAAGAAAGTTGTGAAAATATCGGAATTCAAGTATCCGAAAGCAGCAAACCCAAAGCATGTATATTTCCTTCTTCTCAAACTGTACACTGTCTCAGACAAAGCAGTTATATCCCGGAACTTCTACTGGCTGCATCTGCCTGGAAAAAATTACACACTCTTGGAGCCGTACAGGAAGAAGCAAATACCTCTCAAGATCACTTGCAATGCAGTTATGGTGGGTTCAAGATATGAGCTGGAGGTCAATGTTCATAACACATCTAGAGCTAACTTAGCCAAAAACGTTGTCCAGGAAGACGAAAAACGTGATCTGGGGTTGCTCCAGAAACTCTTTAGTAGATGTGTTGTTTCTGCAGATAGCAACCGTGGTTTGAAAGTAGTGGAAATGAAGGGATCGGATTCAGGAGTTGCTTTCTTCCTTCGTTTCTCAGTTCACAATGCAGAGACAGAGAAACAAGATACGAGGATTCTACCAGTACACTACTCAGACAACTATTTCTCACTAGTTCCAGGTGAATCAATGTCTTTTAAGATCTCATTTGCAGCTCCTACAGGCATGAAGAAGTCTCCTCGTGTTATGCTTCAGGGCTGGAACTACCCTGATAGGTTTAGTGTTTTTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_2 Glyco_hydro_2_C

Protein Analysis

944

Amino Acids

107.66

Weight (kDa)

6.01

Isoelectric Point (pI)

31.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_2_N2 PF22666 39 - 198 1.9e-22 Glycosyl hydrolase 2 galactose-binding domain-like
Glyco_hydro_2 PF00703 209 - 328 1.7e-11 Glycosyl hydrolases family 2
Glyco_hydro_2_C PF02836 341 - 472 7.7e-06 Glycosyl hydrolases family 2, TIM barrel domain
Ig_GlcNase PF18368 853 - 935 1.2e-13 Exo-beta-D-glucosaminidase Ig-fold domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1092
AatII GACGTC 1 cut(s) 408
AccB7I CCANNNNNTGG 1 cut(s) 1982
AccIII TCCGGA 3 cut(s) 184, 236, 1642
AciI CCGC 6 cut(s) 5, 44, 135, 1170, 1400, 2049
AclI AACGTT 2 cut(s) 1108, 2493
AcoI YGGCCR 2 cut(s) 102, 355
AcsI RAATTY 5 cut(s) 272, 313, 941, 1127, 2237
AcuI CTGAAG 3 cut(s) 779, 1620, 2777
AcyI GRCGYC 1 cut(s) 405
AfiI CCNNNNNNNGG 9 cut(s) 120, 197, 488, 491, 824, 1165, 1649, 1703, 1982
AhlI ACTAGT 1 cut(s) 2713
AjnI CCWGG 8 cut(s) 142, 469, 1365, 1804, 2079, 2350, 2499, 2719
AleI CACNNNNGTG 2 cut(s) 164, 1206
AloI GAACNNNNNNTCC 2 cut(s) 1257, 1289
AluBI AGCT 9 cut(s) 82, 370, 530, 680, 1904, 2125, 2446, 2477, 2756
AluI AGCT 9 cut(s) 82, 370, 530, 680, 1904, 2125, 2446, 2477, 2756
Alw21I GWGCWC 2 cut(s) 1330, 1906
Alw26I GTCTC 8 cut(s) 655, 1100, 1670, 1991, 2206, 2308, 2648, 2781
AlwNI CAGNNNCTG 1 cut(s) 1828
Ama87I CYCGRG 1 cut(s) 485
Aor13HI TCCGGA 3 cut(s) 184, 236, 1642
AoxI GGCC 7 cut(s) 102, 355, 493, 620, 881, 1187, 1576
ApeKI GCWGC 7 cut(s) 530, 967, 2040, 2051, 2255, 2341, 2753
ApoI RAATTY 5 cut(s) 272, 313, 941, 1127, 2237
ArsI GACNNNNNNTTYG 4 cut(s) 759, 791, 2481, 2513
Asp700I GAANNNNTTC 2 cut(s) 941, 1395
AspS9I GGNCC 6 cut(s) 574, 620, 882, 1295, 1577, 2158
AsuC2I CCSGG 2 cut(s) 619, 2326
AsuHPI GGTGA 4 cut(s) 178, 515, 607, 2735
AvaI CYCGRG 1 cut(s) 485
AvaII GGWCC 3 cut(s) 574, 1295, 2158
AxyI CCTNAGG 1 cut(s) 1436
BaeGI GKGCMC 1 cut(s) 161
BaeI ACNNNNGTAYC 2 cut(s) 1500, 1533
BalI TGGCCA 1 cut(s) 357
BamHI GGATCC 1 cut(s) 187
BanII GRGCYC 1 cut(s) 1906
BarI GAAGNNNNNNTAC 6 cut(s) 123, 155, 777, 809, 2265, 2297
BauI CACGAG 2 cut(s) 1329, 2781
BbsI GAAGAC 2 cut(s) 23, 2511
Bbv12I GWGCWC 2 cut(s) 1330, 1906
BbvI GCAGC 7 cut(s) 517, 979, 2027, 2038, 2267, 2328, 2765
BccI CCATC 9 cut(s) 113, 121, 332, 429, 886, 1076, 1256, 1365, 1547
BceAI ACGGC 7 cut(s) 88, 122, 1234, 1558, 1591, 2028, 2362
BciT130I CCWGG 8 cut(s) 144, 471, 1367, 1806, 2081, 2352, 2501, 2721
BciVI GTATCC 2 cut(s) 1065, 2256
BclI TGATCA 2 cut(s) 505, 921
BcnI CCSGG 2 cut(s) 619, 2326
BcoDI GTCTC 8 cut(s) 655, 1100, 1670, 1991, 2206, 2308, 2648, 2781
BcuI ACTAGT 1 cut(s) 2713
BfaI CTAG 6 cut(s) 1301, 1491, 1512, 2162, 2472, 2714
BfmI CTRYAG 3 cut(s) 2052, 2562, 2760
BfuI GTATCC 2 cut(s) 1065, 2256
BglII AGATCT 1 cut(s) 2742
BisI GCNGC 9 cut(s) 45, 135, 531, 968, 2041, 2052, 2256, 2342, 2754
BlsI GCNGC 9 cut(s) 46, 136, 532, 969, 2042, 2053, 2257, 2343, 2755
Bme18I GGWCC 3 cut(s) 574, 1295, 2158
BmeT110I CYCGRG 1 cut(s) 485
BmgT120I GGNCC 6 cut(s) 574, 620, 882, 1295, 1577, 2158
BmiI GGNNCC 4 cut(s) 189, 575, 2159, 2376
BmsI GCATC 4 cut(s) 799, 954, 2156, 2353
BpiI GAAGAC 2 cut(s) 23, 2511
BplI GAGNNNNNCTC 2 cut(s) 1637, 1669
BpmI CTGGAG 4 cut(s) 1683, 1827, 2468, 2516
BpuEI CTTGAG 2 cut(s) 807, 2387
BpuMI CCSGG 2 cut(s) 619, 2326
BsaAI YACGTR 1 cut(s) 304
BsaBI GATNNNNATC 2 cut(s) 1052, 1485
BsaHI GRCGYC 1 cut(s) 405
BsaI GGTCTC 2 cut(s) 655, 1991
BsaJI CCNNGG 7 cut(s) 54, 470, 486, 496, 1057, 1976, 2575
BsaWI WCCGGW 4 cut(s) 184, 236, 576, 1642
BsaXI ACNNNNNCTCC 6 cut(s) 82, 112, 466, 496, 1681, 1711
Bsc4I CCNNNNNNNGG 9 cut(s) 120, 197, 488, 491, 824, 1165, 1649, 1703, 1982
Bse21I CCTNAGG 1 cut(s) 1436
Bse3DI GCAATG 1 cut(s) 2422
Bse8I GATNNNNATC 2 cut(s) 1052, 1485
BseAI TCCGGA 3 cut(s) 184, 236, 1642
BseBI CCWGG 8 cut(s) 144, 471, 1367, 1806, 2081, 2352, 2501, 2721
BseDI CCNNGG 7 cut(s) 54, 470, 486, 496, 1057, 1976, 2575
BseJI GATNNNNATC 2 cut(s) 1052, 1485
BseLI CCNNNNNNNGG 9 cut(s) 120, 197, 488, 491, 824, 1165, 1649, 1703, 1982
BseMI GCAATG 1 cut(s) 2422
BseMII CTCAG 6 cut(s) 417, 1340, 2002, 2319, 2652, 2709
BseRI GAGGAG 1 cut(s) 2769
BseSI GKGCMC 1 cut(s) 161
BseXI GCAGC 7 cut(s) 517, 979, 2027, 2038, 2267, 2328, 2765
BseYI CCCAGC 1 cut(s) 1164
BsgI GTGCAG 2 cut(s) 321, 516
BshFI GGCC 7 cut(s) 104, 357, 495, 622, 883, 1189, 1578
BsiHKAI GWGCWC 2 cut(s) 1330, 1906
BsiHKCI CYCGRG 1 cut(s) 485
BsiSI CCGG 7 cut(s) 101, 185, 237, 577, 618, 1643, 2326
BslFI GGGAC 6 cut(s) 257, 487, 566, 587, 1553, 2171
BslI CCNNNNNNNGG 9 cut(s) 120, 197, 488, 491, 824, 1165, 1649, 1703, 1982
BsmAI GTCTC 8 cut(s) 655, 1100, 1670, 1991, 2206, 2308, 2648, 2781
BsmBI CGTCTC 2 cut(s) 1100, 1670
BsmFI GGGAC 6 cut(s) 257, 487, 566, 587, 1553, 2171
BsmI GAATGC 1 cut(s) 1668
BsnI GGCC 7 cut(s) 104, 357, 495, 622, 883, 1189, 1578
Bso31I GGTCTC 2 cut(s) 655, 1991
BsoBI CYCGRG 1 cut(s) 485
Bsp1286I GDGCHC 3 cut(s) 161, 1330, 1906
Bsp13I TCCGGA 3 cut(s) 184, 236, 1642
Bsp1407I TGTACA 2 cut(s) 2068, 2295
Bsp19I CCATGG 1 cut(s) 1976
BspACI CCGC 6 cut(s) 5, 44, 135, 1170, 1400, 2049
BspANI GGCC 7 cut(s) 104, 357, 495, 622, 883, 1189, 1578
BspCNI CTCAG 6 cut(s) 418, 1341, 2001, 2318, 2651, 2708
BspEI TCCGGA 3 cut(s) 184, 236, 1642
BspHI TCATGA 1 cut(s) 508
BspLI GGNNCC 4 cut(s) 189, 575, 2159, 2376
BspMAI CTGCAG 2 cut(s) 2056, 2566
BspTNI GGTCTC 2 cut(s) 655, 1991
BsrDI GCAATG 1 cut(s) 2422
BsrGI TGTACA 2 cut(s) 2068, 2295
BssECI CCNNGG 7 cut(s) 54, 470, 486, 496, 1057, 1976, 2575
BssNI GRCGYC 1 cut(s) 405
BssSI CACGAG 2 cut(s) 1329, 2781
BssT1I CCWWGG 2 cut(s) 496, 1976
Bst2BI CACGAG 2 cut(s) 1329, 2781
Bst2UI CCWGG 8 cut(s) 144, 471, 1367, 1806, 2081, 2352, 2501, 2721
Bst6I CTCTTC 1 cut(s) 1261
BstACI GRCGYC 1 cut(s) 405
BstAUI TGTACA 2 cut(s) 2068, 2295
BstBAI YACGTR 1 cut(s) 304
BstDEI CTNAG 8 cut(s) 426, 1349, 1436, 1988, 2305, 2482, 2638, 2695
BstDSI CCRYGG 4 cut(s) 54, 1057, 1976, 2575
BstENI CCTNNNNNAGG 2 cut(s) 822, 1701
BstMAI GTCTC 8 cut(s) 655, 1100, 1670, 1991, 2206, 2308, 2648, 2781
BstMWI GCNNNNNNNGC 3 cut(s) 2144, 2347, 2483
BstNI CCWGG 8 cut(s) 144, 471, 1367, 1806, 2081, 2352, 2501, 2721
BstNSI RCATGY 2 cut(s) 692, 2273
BstSFI CTRYAG 3 cut(s) 2052, 2562, 2760
BstSLI GKGCMC 1 cut(s) 161
BstV1I GCAGC 7 cut(s) 517, 979, 2027, 2038, 2267, 2328, 2765
BstV2I GAAGAC 2 cut(s) 23, 2511
BstX2I RGATCY 5 cut(s) 50, 187, 1486, 1529, 2742
BstXI CCANNNNNNTGG 2 cut(s) 892, 1707
BstYI RGATCY 5 cut(s) 50, 187, 1486, 1529, 2742
Bsu36I CCTNAGG 1 cut(s) 1436
BsuI GTATCC 2 cut(s) 1065, 2256
BsuRI GGCC 7 cut(s) 104, 357, 495, 622, 883, 1189, 1578
BtgI CCRYGG 4 cut(s) 54, 1057, 1976, 2575
BtgZI GCGATG 1 cut(s) 1269
BtsIMutI CAGTG 5 cut(s) 408, 924, 1248, 1569, 2298
CaiI CAGNNNCTG 1 cut(s) 1828
CciI TCATGA 1 cut(s) 508
Cfr13I GGNCC 6 cut(s) 574, 620, 882, 1295, 1577, 2158
DdeI CTNAG 8 cut(s) 426, 1349, 1436, 1988, 2305, 2482, 2638, 2695
DraI TTTAAA 3 cut(s) 847, 1612, 2194
DrdI GACNNNNNNGTC 1 cut(s) 1092
DseDI GACNNNNNNGTC 1 cut(s) 1092
EaeI YGGCCR 2 cut(s) 102, 355
Eam1104I CTCTTC 1 cut(s) 1261
EarI CTCTTC 1 cut(s) 1261
EciI GGCGGA 2 cut(s) 20, 1389
Ecl136II GAGCTC 1 cut(s) 1904
Eco130I CCWWGG 2 cut(s) 496, 1976
Eco24I GRGCYC 1 cut(s) 1906
Eco31I GGTCTC 2 cut(s) 655, 1991
Eco32I GATATC 1 cut(s) 1213
Eco47I GGWCC 3 cut(s) 574, 1295, 2158
Eco53kI GAGCTC 1 cut(s) 1904
Eco57I CTGAAG 3 cut(s) 779, 1620, 2777
Eco81I CCTNAGG 1 cut(s) 1436
Eco88I CYCGRG 1 cut(s) 485
EcoICRI GAGCTC 1 cut(s) 1904
EcoNI CCTNNNNNAGG 2 cut(s) 822, 1701
EcoO109I RGGNCCY 1 cut(s) 2158
EcoRI GAATTC 2 cut(s) 941, 2237
EcoRII CCWGG 8 cut(s) 142, 469, 1365, 1804, 2079, 2350, 2499, 2719
EcoRV GATATC 1 cut(s) 1213
EcoT14I CCWWGG 2 cut(s) 496, 1976
EcoT38I GRGCYC 1 cut(s) 1906
ErhI CCWWGG 2 cut(s) 496, 1976
Esp3I CGTCTC 2 cut(s) 1100, 1670
FalI AAGNNNNNCTT 2 cut(s) 1550, 1582
FaqI GGGAC 6 cut(s) 257, 487, 566, 587, 1553, 2171
FauNDI CATATG 1 cut(s) 590
FbaI TGATCA 2 cut(s) 505, 921
Fnu4HI GCNGC 9 cut(s) 45, 135, 531, 968, 2041, 2052, 2256, 2342, 2754
FriOI GRGCYC 1 cut(s) 1906
Fsp4HI GCNGC 9 cut(s) 45, 135, 531, 968, 2041, 2052, 2256, 2342, 2754
FspBI CTAG 6 cut(s) 1301, 1491, 1512, 2162, 2472, 2714
GluI GCNGC 9 cut(s) 45, 135, 531, 968, 2041, 2052, 2256, 2342, 2754
GsaI CCCAGC 1 cut(s) 1168
GsuI CTGGAG 4 cut(s) 1683, 1827, 2468, 2516
HaeIII GGCC 7 cut(s) 104, 357, 495, 622, 883, 1189, 1578
HapII CCGG 7 cut(s) 101, 185, 237, 577, 618, 1643, 2326
Hin1I GRCGYC 1 cut(s) 405
HincII GTYRAC 1 cut(s) 70
HindII GTYRAC 1 cut(s) 70
HpaII CCGG 7 cut(s) 101, 185, 237, 577, 618, 1643, 2326
HphI GGTGA 4 cut(s) 178, 515, 607, 2735
Hpy166II GTNNAC 8 cut(s) 70, 1885, 1935, 1944, 2107, 2298, 2644, 2689
Hpy8I GTNNAC 8 cut(s) 70, 1885, 1935, 1944, 2107, 2298, 2644, 2689
HpyAV CCTTC 8 cut(s) 234, 667, 1014, 1697, 1864, 2291, 2593, 2639
HpyCH4IV ACGT 7 cut(s) 303, 405, 829, 1093, 1108, 2493, 2515
HpyF10VI GCNNNNNNNGC 3 cut(s) 2144, 2347, 2483
HpyF3I CTNAG 8 cut(s) 426, 1349, 1436, 1988, 2305, 2482, 2638, 2695
HpySE526I ACGT 7 cut(s) 303, 405, 829, 1093, 1108, 2493, 2515
Hsp92I GRCGYC 1 cut(s) 405
Kpn2I TCCGGA 3 cut(s) 184, 236, 1642
Ksp22I TGATCA 2 cut(s) 505, 921
LmnI GCTCC 3 cut(s) 2374, 2535, 2761
Lsp1109I GCAGC 7 cut(s) 517, 979, 2027, 2038, 2267, 2328, 2765
LweI GCATC 4 cut(s) 799, 954, 2156, 2353
MaeI CTAG 6 cut(s) 1301, 1491, 1512, 2162, 2472, 2714
MaeII ACGT 7 cut(s) 303, 405, 829, 1093, 1108, 2493, 2515
MaeIII GTNAC 7 cut(s) 406, 613, 691, 1009, 1089, 1109, 2087
MfeI CAATTG 3 cut(s) 1066, 1463, 2072
MflI RGATCY 5 cut(s) 50, 187, 1486, 1529, 2742
MhlI GDGCHC 3 cut(s) 161, 1330, 1906
MlsI TGGCCA 1 cut(s) 357
MluNI TGGCCA 1 cut(s) 357
MmeI TCCRAC 4 cut(s) 1639, 1688, 1898, 2109
Mox20I TGGCCA 1 cut(s) 357
MroI TCCGGA 3 cut(s) 184, 236, 1642
MroXI GAANNNNTTC 2 cut(s) 941, 1395
MscI TGGCCA 1 cut(s) 357
MseI TTAA 5 cut(s) 846, 1611, 1749, 2193, 2739
MslI CAYNNNNRTG 4 cut(s) 164, 1151, 1206, 2152
Msp20I TGGCCA 1 cut(s) 357
MspA1I CMGCKG 1 cut(s) 2051
MspI CCGG 7 cut(s) 101, 185, 237, 577, 618, 1643, 2326
MunI CAATTG 3 cut(s) 1066, 1463, 2072
Mva1269I GAATGC 1 cut(s) 1668
MvaI CCWGG 8 cut(s) 144, 471, 1367, 1806, 2081, 2352, 2501, 2721
MwoI GCNNNNNNNGC 3 cut(s) 2144, 2347, 2483
NciI CCSGG 2 cut(s) 619, 2326
NcoI CCATGG 1 cut(s) 1976
NdeI CATATG 1 cut(s) 590
NlaIV GGNNCC 4 cut(s) 189, 575, 2159, 2376
NmuCI GTSAC 3 cut(s) 406, 613, 691
NspI RCATGY 2 cut(s) 692, 2273
OliI CACNNNNGTG 2 cut(s) 164, 1206
PagI TCATGA 1 cut(s) 508
PcsI WCGNNNNNNNCGW 1 cut(s) 1685
PctI GAATGC 1 cut(s) 1668
PdmI GAANNNNTTC 2 cut(s) 941, 1395
PflMI CCANNNNNTGG 1 cut(s) 1982
PfoI TCCNGGA 3 cut(s) 1804, 2324, 2499
PkrI GCNGC 9 cut(s) 46, 136, 532, 969, 2042, 2053, 2257, 2343, 2755
Ppu21I YACGTR 1 cut(s) 304
PpuMI RGGWCCY 1 cut(s) 2158
Psp124BI GAGCTC 1 cut(s) 1906
Psp1406I AACGTT 2 cut(s) 1108, 2493
Psp5II RGGWCCY 1 cut(s) 2158
Psp6I CCWGG 8 cut(s) 142, 469, 1365, 1804, 2079, 2350, 2499, 2719
PspFI CCCAGC 1 cut(s) 1164
PspGI CCWGG 8 cut(s) 142, 469, 1365, 1804, 2079, 2350, 2499, 2719
PspN4I GGNNCC 4 cut(s) 189, 575, 2159, 2376
PspPI GGNCC 6 cut(s) 574, 620, 882, 1295, 1577, 2158
PspPPI RGGWCCY 1 cut(s) 2158
PsrI GAACNNNNNNTAC 2 cut(s) 2162, 2194
PstI CTGCAG 2 cut(s) 2056, 2566
PstNI CAGNNNCTG 1 cut(s) 1828
PsuI RGATCY 5 cut(s) 50, 187, 1486, 1529, 2742
RseI CAYNNNNRTG 4 cut(s) 164, 1151, 1206, 2152
SacI GAGCTC 1 cut(s) 1906
SaqAI TTAA 5 cut(s) 846, 1611, 1749, 2193, 2739
SatI GCNGC 9 cut(s) 45, 135, 531, 968, 2041, 2052, 2256, 2342, 2754
Sau96I GGNCC 6 cut(s) 574, 620, 882, 1295, 1577, 2158
SduI GDGCHC 3 cut(s) 161, 1330, 1906
SfaNI GCATC 4 cut(s) 799, 954, 2156, 2353
SfcI CTRYAG 3 cut(s) 2052, 2562, 2760
SinI GGWCC 3 cut(s) 574, 1295, 2158
SmiMI CAYNNNNRTG 4 cut(s) 164, 1151, 1206, 2152
SmlI CTYRAG 2 cut(s) 822, 2402
SmoI CTYRAG 2 cut(s) 822, 2402
SpeI ACTAGT 1 cut(s) 2713
SsiI CCGC 6 cut(s) 5, 44, 135, 1170, 1400, 2049
SspI AATATT 1 cut(s) 745
SspMI CTAG 6 cut(s) 1301, 1491, 1512, 2162, 2472, 2714
SstI GAGCTC 1 cut(s) 1906
StyI CCWWGG 2 cut(s) 496, 1976
TaiI ACGT 7 cut(s) 306, 408, 832, 1096, 1111, 2496, 2518
TaqI TCGA 6 cut(s) 63, 765, 1765, 1854, 1911, 2016
TatI WGTACW 6 cut(s) 1785, 1896, 1941, 2068, 2295, 2686
TauI GCSGC 2 cut(s) 47, 137
Tru1I TTAA 5 cut(s) 846, 1611, 1749, 2193, 2739
Tru9I TTAA 5 cut(s) 846, 1611, 1749, 2193, 2739
TscAI CASTG 5 cut(s) 415, 931, 1255, 1576, 2305
TseFI GTSAC 3 cut(s) 406, 613, 691
TseI GCWGC 7 cut(s) 530, 967, 2040, 2051, 2255, 2341, 2753
Tsp45I GTSAC 3 cut(s) 406, 613, 691
TspGWI ACGGA 5 cut(s) 71, 308, 1046, 1053, 1148
TspRI CASTG 5 cut(s) 415, 931, 1255, 1576, 2305
Van91I CCANNNNNTGG 1 cut(s) 1982
VpaK11BI GGWCC 3 cut(s) 574, 1295, 2158
XagI CCTNNNNNAGG 2 cut(s) 822, 1701
XapI RAATTY 5 cut(s) 272, 313, 941, 1127, 2237
XbaI TCTAGA 1 cut(s) 2471
XceI RCATGY 2 cut(s) 692, 2273
XcmI CCANNNNNNNNNTGG 1 cut(s) 1767
XmnI GAANNNNTTC 2 cut(s) 941, 1395
XspI CTAG 6 cut(s) 1301, 1491, 1512, 2162, 2472, 2714
ZraI GACGTC 1 cut(s) 406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.