Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0006337

Physical Location & Seq

Genomic Coordinates Forward (+)

29626793 .. 29639663

Gene Structure 12871 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1218800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2925 bp

ATGGCGGCGATTGGGAAGACGAAGCTGGACTCCGGGTGGCTCGCGGCGAGGTCAACGGAGGTCAGTCTCAGTGGGATTCAGCTCACCACCACCCACCCTCCCTCAATTGGCACATCCCCTTGGATGGAGGCCGTTGTTCCCGGAACTGTTTTGGCAACCCTGGTGAAGAACAAAGTTGTACCAGACCCGTTCTACGGGTTAAAGAACGAGTCAATCATAGACATTGCTGATTCCGGGAGGGAGTACTACACGTTCTGGTTCTTTACAACTTTTCGCTGTAAACTGTCAGGTACTCAGCACCTTGATCTGAATTTCCGCGCAATCAACTACTCTGCGGAGGTATATTTAAATGGGCACAAAACGGTCCTGCCAAAAGGGATGTTCCGAAGGCATTCTCTTGATGTCACTGATGTAGTGCATCCTGGTGGTGAAAACTTGCTTGCCGTTCTTGTTTACCCTCCGGATCATCCGGGGAGTATTCCTCCTGAGGGTGGGCAAGGCGGTGATCATGAGATCGGGAAAGATGTAGCAACGCAGTATGTGGAGGGTTGGGATTGGATGTGTCCTATAAGGGATAGAAACACCGGCATATGGGATGAGGTGTCGATTTCCGTAACAGGGCCAGTAAAAATAATTGACCCTCACTTGGTTTCAACCTTCTTCGACAATTACAAGAGGGTATATTTGCATACTACTACTGAGTTGGAAAATAAGAGCACCCAGTCTGCGGAGTGTTCACTGAATATCCAAGTAACAACAGAACTTGAAGGAAATTTTTGTTTAGTAGAGCATGTTCAGACTCAACATTTGTCAATCCCTGCTGGATCCCGAGTGCACTATACTTTTCCCGAGCTCTTTTTCTATAAACCTAATCTATGGTGGCCAAATGGAATGGGAAAGCAATCCTTGTACAAAGTATCTATTACTGTTGATGTAAAGGGATATGGAGAGTCTGATTTGTGGGGCCAATTATTTGGTTTTCGTAAAATTCACAGCTACATTGATACTGTCACAGGAGGAAGGTTGTTTAAGGTCAATGGTCAGCCTATTTTTATTCGTGGCGGTAATTGGATATTGTCAGATGGCCTCCTCCGACTTTCAAAGAAGCGTTACAAGACTGACATCAAGTTCCATGCAGATATGAATTTTAACATGATCCGTTGTTGGGGCGGTGGGCTGGCTGAGAGGCCGGACTTTTATCATTACTGTGACATTTATGGTTTGTTGGTCTGGCAAGAATTTTGGATTACAGGAGATGTCGATGGACGAGGTATCCCTGTATCAAATCCAGATGGTCCGCTGGACCATGATCTTTTCTTGCTAAGTGCAAGAGATACTGTCAAGCTTCTCAGGAACCATCCCAGTCTTGCCCTTTGGGTGGGCGGAAATGAGCAAGTTCCACCAGATGACATCAACACGGCTTTGAAAAATGACCTCAGGCTCCATCCGCATTTCGAAAAATCGGTAAATGAAAGTGGCAAGTCCACTGAAGATTTGTCCCCAGTGTTGAGGGATCCAAGCCAATATCTTGATGGTACGCGTGTTTACATCCAAGGATCCATGTGGGATGGGTTTGCAAATGGAAAGGGGGACTTCACCGATGGTCCTTATGAAATTCAAAATCCCGAAGACTTCTTTAAAGATGACTATTACAAATACGGGTTCAATCCTGAAGTTGGTTCTGTAGGTATGCCGGTTTCAGCTACCATCAGAGCCACAATGCCTCCTGAAGGATGGCAAATTCCTTTGTTTAAGAAGGTTTCCGAATATTACGAAGAAGTTCCAAACCCCATATGGGAATACCATAAATATATCCCTTACTCTAAACCAGGCAAGGTTCATGATCAGATTTTACTTTACGGGTCGCAACCAAAGGATCTTGATGATTTCTGCTTGAAGGCTCAACTTGTGAACTACATTCAGTATAGAGCTCTACTGGAGGGTTGGACTTCCCGAATGTGGACTAAATACACCGGTGTTTTGATTTGGAAAACACAGAATCCATGGACAGGTCTTAGGGGTCAATTTTATGATCATCTTCTAGACCAAACGGCAGGTTTCTATGGCTGTCGCTGTGCTGCGGAGCCAATCCACGTCCAACTTAACTTAGCTACATATCTTATAGAGGTTGTCAACACTACATCAGAGGAACTATCTGACATCGCTATAGAAGCCTCGGTATGGGATCTAGAAGGAACATGCCCATACTACAAAGTTCACGAAATGGTGTCAGTGCCCCCAAAAAGAACAGTGCCAATTGCTGAGATGATATATCCAAAATCTAAAAACCCGAAGCCAGTTTACTTTCTTCTTCTCAAACTGTATCACAAGTCAGACTACAGGATTATTTCCAGGAACTTTTACTGGTTGCATCTGTCGGGTGGGGATTACAAGCTGTTAGAACCATACAGGAAGAAAACAGTGCCACTCAAGTTTACATCCGCAGTTTTCATCAAGGGAACCACCTACGAAATGCATATCCGTGTCCAGAACACATCGAAGAAACCAGATGCCAAGACTTTAACCTATCAAAACAATTTCACTACTAAACAAGGTGACGGCGATTTTGATACAGCCTCGGTGGATTGTGTACAGGATGGAGCAGGTGCAAAGAACGAAGTTAGTTGGCTTCAGAAAATATCCAGACGTTTCGCAATGGAGACTGATGATTTGAAAGTTGCTGAAATCAATGGCGCCAACATTGGCGTTGCTTTCTTCCTTCATTTTTCTGTTCATGGAGTGAAGAAGAACCATAAGGAAGGAGAGGACACAAGAATTCTTCCCGTTCATTACTCGGACAATTATTTTTCACTGGTGCCAGGTGAGGCTATGCCCATCAAGATCTCTTTTGAGGTCCCTCCCGGTGTCACGCCCCGAGTTACTCTCGCCGGATGGAATTACCATGGTGTCCATACAGTTCATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Glyco_hydro_2 Glyco_hydro_2_C

Protein Analysis

975

Amino Acids

110.46

Weight (kDa)

6.24

Isoelectric Point (pI)

31.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_2_N2	PF22666	39 - 198	3.6e-22	Glycosyl hydrolase 2 galactose-binding domain-like
Glyco_hydro_2	PF00703	210 - 328	3.9e-09	Glycosyl hydrolases family 2
Ig_GlcNase	PF18368	903 - 966	3.3e-09	Exo-beta-D-glucosaminidase Ig-fold domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006337)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G09010
fragaria_vesca	FvH4_6g05900 FvH4_6g05900 FvH4_6g05900 FvH4_6g05900 FvH4_6g05900 FvH4_6g05900
malus_domestica	MD12G1218800.v1.1 MD12G1218900.v1.1
prunus_persica	Prupe.6G325300_v2.0.a1 Prupe.6G325300_v2.0.a1 Prupe.6G325400_v2.0.a1
pyrus_communis	pycom12g20450 pycom12g20460
rosa_chinensis	RchiOBHm_Chr3g0454891
rosa_laevigata	RLG00000025403
rosa_multiflora	Rmu_sc0001792.1_g000010 Rmu_sc0013126.1_g000001 Rmu_sc0036267.1_g000001
rosa_roxburghii	Rroxscaffold_6G00424690
rosa_rugosa	Rorug03G0002800 Rorug03G0002800
rosa_samantha	Rh3AG062700 Rh3BG064600 Rh3CG063500 Rh3DG064600
rosa_wichuraiana	Rw3G004900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2594
Acc36I	ACCTGC	2 cut(s)	2045, 2594
AccB1I	GGYRCC	2 cut(s)	2693, 2815
AccII	CGCG	3 cut(s)	44, 318, 1540
AccIII	TCCGGA	1 cut(s)	460
AcoI	YGGCCR	1 cut(s)	881
AcsI	RAATTY	8 cut(s)	310, 772, 987, 1144, 1238, 1614, 1740, 2775
AcuI	CTGAAG	4 cut(s)	1509, 1692, 1749, 2615
AcyI	GRCGYC	1 cut(s)	2694
AfaI	GTAC	6 cut(s)	180, 245, 292, 911, 1537, 2592
AflIII	ACRYGT	2 cut(s)	249, 1538
AgeI	ACCGGT	1 cut(s)	1973
AgsI	TTSAA	8 cut(s)	654, 767, 1101, 1426, 1619, 1666, 1897, 2674
AjiI	CACGTC	1 cut(s)	2095
AjnI	CCWGG	5 cut(s)	159, 421, 1828, 2351, 2818
AluBI	AGCT	9 cut(s)	25, 82, 853, 996, 1345, 1703, 1931, 2111, 2395
AluI	AGCT	9 cut(s)	25, 82, 853, 996, 1345, 1703, 1931, 2111, 2395
Alw21I	GWGCWC	4 cut(s)	719, 837, 855, 1933
Alw26I	GTCTC	2 cut(s)	71, 2654
Alw44I	GTGCAC	1 cut(s)	833
Ama87I	CYCGRG	3 cut(s)	828, 848, 2874
Aor13HI	TCCGGA	1 cut(s)	460
AoxI	GGCC	6 cut(s)	129, 620, 881, 964, 1084, 1187
ApaLI	GTGCAC	1 cut(s)	833
ApeKI	GCWGC	1 cut(s)	2078
ApoI	RAATTY	8 cut(s)	310, 772, 987, 1144, 1238, 1614, 1740, 2775
ArsI	GACNNNNNNTTYG	6 cut(s)	790, 822, 2549, 2581, 2653, 2685
AsiGI	ACCGGT	1 cut(s)	1973
Asp700I	GAANNNNTTC	2 cut(s)	391, 1779
AspLEI	GCGC	2 cut(s)	320, 2696
AspS9I	GGNCC	7 cut(s)	364, 620, 964, 1295, 1303, 1604, 2854
AsuC2I	CCSGG	5 cut(s)	34, 141, 235, 471, 2862
AsuHPI	GGTGA	7 cut(s)	76, 175, 440, 515, 1588, 2567, 2834
AsuII	TTCGAA	1 cut(s)	1455
AvaI	CYCGRG	3 cut(s)	828, 848, 2874
AvaII	GGWCC	5 cut(s)	364, 1295, 1303, 1604, 2854
AxyI	CCTNAGG	2 cut(s)	486, 1436
BaeGI	GKGCMC	3 cut(s)	357, 837, 2238
BaeI	ACNNNNGTAYC	2 cut(s)	1527, 1560
BalI	TGGCCA	1 cut(s)	883
BamHI	GGATCC	3 cut(s)	824, 1513, 1556
BanI	GGYRCC	2 cut(s)	2693, 2815
BanII	GRGCYC	2 cut(s)	855, 1933
BarI	GAAGNNNNNNTAC	2 cut(s)	2285, 2317
BbsI	GAAGAC	2 cut(s)	23, 1635
Bbv12I	GWGCWC	4 cut(s)	719, 837, 855, 1933
BbvI	GCAGC	1 cut(s)	2065
BceAI	ACGGC	5 cut(s)	116, 428, 1434, 2067, 2575
BciT130I	CCWGG	5 cut(s)	161, 423, 1830, 2353, 2820
BciVI	GTATCC	1 cut(s)	1283
BclI	TGATCA	3 cut(s)	505, 1843, 2032
BcnI	CCSGG	5 cut(s)	34, 141, 235, 471, 2862
BcoDI	GTCTC	2 cut(s)	71, 2654
BfaI	CTAG	2 cut(s)	2042, 2189
BfmI	CTRYAG	3 cut(s)	1683, 2166, 2338
BfoI	RGCGCY	1 cut(s)	2697
BfuAI	ACCTGC	2 cut(s)	2045, 2594
BfuI	GTATCC	1 cut(s)	1283
BglII	AGATCT	1 cut(s)	2841
BisI	GCNGC	3 cut(s)	6, 45, 2079
BlsI	GCNGC	3 cut(s)	7, 46, 2080
BmcAI	AGTACT	1 cut(s)	245
Bme18I	GGWCC	5 cut(s)	364, 1295, 1303, 1604, 2854
BmeT110I	CYCGRG	3 cut(s)	828, 848, 2874
BmgBI	CACGTC	1 cut(s)	2095
BmgT120I	GGNCC	7 cut(s)	364, 620, 964, 1295, 1303, 1604, 2854
BmrI	ACTGGG	3 cut(s)	715, 1356, 1496
BmsI	GCATC	3 cut(s)	427, 2380, 2500
BmuI	ACTGGG	3 cut(s)	715, 1356, 1496
BpiI	GAAGAC	2 cut(s)	23, 1635
BplI	GAGNNNNNCTC	4 cut(s)	466, 498, 2868, 2900
BpmI	CTGGAG	1 cut(s)	1958
Bpu14I	TTCGAA	1 cut(s)	1455
BpuEI	CTTGAG	1 cut(s)	2414
BpuMI	CCSGG	5 cut(s)	34, 141, 235, 471, 2862
BsaHI	GRCGYC	1 cut(s)	2694
BsaJI	CCNNGG	8 cut(s)	119, 159, 470, 1552, 2003, 2175, 2577, 2902
BsaWI	WCCGGW	2 cut(s)	460, 1973
BsaXI	ACNNNNNCTCC	4 cut(s)	82, 112, 1708, 1738
Bse118I	RCCGGY	3 cut(s)	584, 1693, 1973
Bse1I	ACTGG	8 cut(s)	623, 721, 1362, 1502, 1941, 2297, 2369, 2817
Bse21I	CCTNAGG	2 cut(s)	486, 1436
Bse3DI	GCAATG	2 cut(s)	222, 2661
BseAI	TCCGGA	1 cut(s)	460
BseBI	CCWGG	5 cut(s)	161, 423, 1830, 2353, 2820
BseDI	CCNNGG	8 cut(s)	119, 159, 470, 1552, 2003, 2175, 2577, 2902
BseMI	GCAATG	2 cut(s)	222, 2661
BseMII	CTCAG	8 cut(s)	82, 308, 477, 690, 1173, 1363, 1450, 2253
BseNI	ACTGG	8 cut(s)	623, 721, 1362, 1502, 1941, 2297, 2369, 2817
BseRI	GAGGAG	1 cut(s)	1079
BseSI	GKGCMC	3 cut(s)	357, 837, 2238
BseXI	GCAGC	1 cut(s)	2065
Bsh1236I	CGCG	3 cut(s)	44, 318, 1540
BshFI	GGCC	6 cut(s)	131, 622, 883, 966, 1086, 1189
BshNI	GGYRCC	2 cut(s)	2693, 2815
BshTI	ACCGGT	1 cut(s)	1973
BsiHKAI	GWGCWC	4 cut(s)	719, 837, 855, 1933
BsiHKCI	CYCGRG	3 cut(s)	828, 848, 2874
BslFI	GGGAC	3 cut(s)	1483, 1604, 2840
BsmAI	GTCTC	2 cut(s)	71, 2654
BsmFI	GGGAC	3 cut(s)	1483, 1604, 2840
BsmI	GAATGC	1 cut(s)	391
BsnI	GGCC	6 cut(s)	131, 622, 883, 966, 1086, 1189
BsoBI	CYCGRG	3 cut(s)	828, 848, 2874
Bsp119I	TTCGAA	1 cut(s)	1455
Bsp1286I	GDGCHC	6 cut(s)	357, 719, 837, 855, 1933, 2238
Bsp13I	TCCGGA	1 cut(s)	460
Bsp1407I	TGTACA	2 cut(s)	909, 2590
Bsp19I	CCATGG	2 cut(s)	2003, 2902
BspANI	GGCC	6 cut(s)	131, 622, 883, 966, 1086, 1189
BspCNI	CTCAG	8 cut(s)	81, 307, 478, 691, 1174, 1362, 1449, 2254
BspEI	TCCGGA	1 cut(s)	460
BspFNI	CGCG	3 cut(s)	44, 318, 1540
BspHI	TCATGA	2 cut(s)	508, 1840
BspMI	ACCTGC	2 cut(s)	2045, 2594
BspT104I	TTCGAA	1 cut(s)	1455
BspT107I	GGYRCC	2 cut(s)	2693, 2815
BsrDI	GCAATG	2 cut(s)	222, 2661
BsrFI	RCCGGY	3 cut(s)	584, 1693, 1973
BsrGI	TGTACA	2 cut(s)	909, 2590
BsrI	ACTGG	8 cut(s)	623, 721, 1362, 1502, 1941, 2297, 2369, 2817
BssAI	RCCGGY	3 cut(s)	584, 1693, 1973
BssECI	CCNNGG	8 cut(s)	119, 159, 470, 1552, 2003, 2175, 2577, 2902
BssNI	GRCGYC	1 cut(s)	2694
BssT1I	CCWWGG	4 cut(s)	119, 1552, 2003, 2902
Bst2UI	CCWGG	5 cut(s)	161, 423, 1830, 2353, 2820
BstACI	GRCGYC	1 cut(s)	2694
BstAUI	TGTACA	2 cut(s)	909, 2590
BstBI	TTCGAA	1 cut(s)	1455
BstC8I	GCNNGC	3 cut(s)	42, 441, 1179
BstDSI	CCRYGG	2 cut(s)	2003, 2902
BstENI	CCTNNNNNAGG	1 cut(s)	1728
BstFNI	CGCG	3 cut(s)	44, 318, 1540
BstH2I	RGCGCY	1 cut(s)	2697
BstHHI	GCGC	2 cut(s)	320, 2696
BstMAI	GTCTC	2 cut(s)	71, 2654
BstMWI	GCNNNNNNNGC	2 cut(s)	1447, 2171
BstNI	CCWGG	5 cut(s)	161, 423, 1830, 2353, 2820
BstNSI	RCATGY	2 cut(s)	794, 2202
BstSFI	CTRYAG	3 cut(s)	1683, 2166, 2338
BstSLI	GKGCMC	3 cut(s)	357, 837, 2238
BstUI	CGCG	3 cut(s)	44, 318, 1540
BstV1I	GCAGC	1 cut(s)	2065
BstV2I	GAAGAC	2 cut(s)	23, 1635
BstX2I	RGATCY	6 cut(s)	824, 1513, 1556, 1876, 2185, 2841
BstXI	CCANNNNNNTGG	1 cut(s)	974
BstYI	RGATCY	6 cut(s)	824, 1513, 1556, 1876, 2185, 2841
Bsu36I	CCTNAGG	2 cut(s)	486, 1436
BsuI	GTATCC	1 cut(s)	1283
BsuRI	GGCC	6 cut(s)	131, 622, 883, 966, 1086, 1189
BtgI	CCRYGG	2 cut(s)	2003, 2902
BtgZI	GCGATG	1 cut(s)	2146
BtrI	CACGTC	1 cut(s)	2095
BtsIMutI	CAGTG	9 cut(s)	76, 405, 737, 1485, 1509, 2238, 2256, 2427, 2810
BveI	ACCTGC	2 cut(s)	2045, 2594
Cac8I	GCNNGC	3 cut(s)	42, 441, 1179
CciI	TCATGA	2 cut(s)	508, 1840
CfoI	GCGC	2 cut(s)	320, 2696
Cfr10I	RCCGGY	3 cut(s)	584, 1693, 1973
Cfr13I	GGNCC	7 cut(s)	364, 620, 964, 1295, 1303, 1604, 2854
Csp6I	GTAC	6 cut(s)	179, 244, 291, 910, 1536, 2591
CspAI	ACCGGT	1 cut(s)	1973
CviQI	GTAC	6 cut(s)	179, 244, 291, 910, 1536, 2591
DinI	GGCGCC	1 cut(s)	2695
DraI	TTTAAA	2 cut(s)	348, 1639
EaeI	YGGCCR	1 cut(s)	881
EciI	GGCGGA	1 cut(s)	1398
Ecl136II	GAGCTC	2 cut(s)	853, 1931
Eco130I	CCWWGG	4 cut(s)	119, 1552, 2003, 2902
Eco24I	GRGCYC	2 cut(s)	855, 1933
Eco47I	GGWCC	5 cut(s)	364, 1295, 1303, 1604, 2854
Eco53kI	GAGCTC	2 cut(s)	853, 1931
Eco57I	CTGAAG	4 cut(s)	1509, 1692, 1749, 2615
Eco81I	CCTNAGG	2 cut(s)	486, 1436
Eco88I	CYCGRG	3 cut(s)	828, 848, 2874
EcoICRI	GAGCTC	2 cut(s)	853, 1931
EcoNI	CCTNNNNNAGG	1 cut(s)	1728
EcoO109I	RGGNCCY	1 cut(s)	2854
EcoRI	GAATTC	1 cut(s)	2775
EcoRII	CCWGG	5 cut(s)	159, 421, 1828, 2351, 2818
EcoT14I	CCWWGG	4 cut(s)	119, 1552, 2003, 2902
EcoT22I	ATGCAT	1 cut(s)	2478
EcoT38I	GRGCYC	2 cut(s)	855, 1933
EgeI	GGCGCC	1 cut(s)	2695
EheI	GGCGCC	1 cut(s)	2695
ErhI	CCWWGG	4 cut(s)	119, 1552, 2003, 2902
FalI	AAGNNNNNCTT	4 cut(s)	890, 922, 1577, 1609
FaqI	GGGAC	3 cut(s)	1483, 1604, 2840
FauNDI	CATATG	2 cut(s)	590, 1793
FbaI	TGATCA	3 cut(s)	505, 1843, 2032
Fnu4HI	GCNGC	3 cut(s)	6, 45, 2079
FriOI	GRGCYC	2 cut(s)	855, 1933
Fsp4HI	GCNGC	3 cut(s)	6, 45, 2079
FspBI	CTAG	2 cut(s)	2042, 2189
GlaI	GCGC	2 cut(s)	319, 2695
GluI	GCNGC	3 cut(s)	6, 45, 2079
GsuI	CTGGAG	1 cut(s)	1958
HaeII	RGCGCY	1 cut(s)	2697
HaeIII	GGCC	6 cut(s)	131, 622, 883, 966, 1086, 1189
HhaI	GCGC	2 cut(s)	320, 2696
Hin1I	GRCGYC	1 cut(s)	2694
Hin6I	GCGC	2 cut(s)	318, 2694
HinP1I	GCGC	2 cut(s)	318, 2694
HincII	GTYRAC	2 cut(s)	54, 2134
HindII	GTYRAC	2 cut(s)	54, 2134
HindIII	AAGCTT	1 cut(s)	1343
HinfI	GANTC	7 cut(s)	29, 76, 209, 230, 799, 950, 1999
HphI	GGTGA	7 cut(s)	76, 175, 440, 515, 1588, 2567, 2834
HpyCH4IV	ACGT	3 cut(s)	251, 2094, 2647
HpyCH4V	TGCA	9 cut(s)	418, 688, 835, 1136, 1328, 1577, 2371, 2476, 2609
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1447, 2171
HpySE526I	ACGT	3 cut(s)	251, 2094, 2647
Hsp92I	GRCGYC	1 cut(s)	2694
HspAI	GCGC	2 cut(s)	318, 2694
KasI	GGCGCC	1 cut(s)	2693
Kpn2I	TCCGGA	1 cut(s)	460
Ksp22I	TGATCA	3 cut(s)	505, 1843, 2032
LmnI	GCTCC	3 cut(s)	1446, 2083, 2600
Lsp1109I	GCAGC	1 cut(s)	2065
LweI	GCATC	3 cut(s)	427, 2380, 2500
MaeI	CTAG	2 cut(s)	2042, 2189
MaeII	ACGT	3 cut(s)	251, 2094, 2647
MaeIII	GTNAC	9 cut(s)	403, 613, 751, 1009, 1109, 1208, 2555, 2866, 2878
MfeI	CAATTG	2 cut(s)	105, 2256
MflI	RGATCY	6 cut(s)	824, 1513, 1556, 1876, 2185, 2841
MhlI	GDGCHC	6 cut(s)	357, 719, 837, 855, 1933, 2238
MlsI	TGGCCA	1 cut(s)	883
MluI	ACGCGT	1 cut(s)	1538
MluNI	TGGCCA	1 cut(s)	883
Mly113I	GGCGCC	1 cut(s)	2694
MlyI	GAGTC	4 cut(s)	23, 218, 793, 959
MmeI	TCCRAC	4 cut(s)	684, 1117, 1925, 2122
Mox20I	TGGCCA	1 cut(s)	883
Mph1103I	ATGCAT	1 cut(s)	2478
MroI	TCCGGA	1 cut(s)	460
MroXI	GAANNNNTTC	2 cut(s)	391, 1779
MscI	TGGCCA	1 cut(s)	883
MseI	TTAA	8 cut(s)	200, 347, 1029, 1149, 1638, 1752, 2103, 2522
MslI	CAYNNNNRTG	3 cut(s)	423, 1206, 2481
Msp20I	TGGCCA	1 cut(s)	883
MspA1I	CMGCKG	1 cut(s)	1300
MunI	CAATTG	2 cut(s)	105, 2256
Mva1269I	GAATGC	1 cut(s)	391
MvaI	CCWGG	5 cut(s)	161, 423, 1830, 2353, 2820
MvnI	CGCG	3 cut(s)	44, 318, 1540
MwoI	GCNNNNNNNGC	2 cut(s)	1447, 2171
NarI	GGCGCC	1 cut(s)	2694
NciI	CCSGG	5 cut(s)	34, 141, 235, 471, 2862
NcoI	CCATGG	2 cut(s)	2003, 2902
NdeI	CATATG	2 cut(s)	590, 1793
NmuCI	GTSAC	5 cut(s)	403, 1009, 1208, 2555, 2866
NsiI	ATGCAT	1 cut(s)	2478
NspI	RCATGY	2 cut(s)	794, 2202
NspV	TTCGAA	1 cut(s)	1455
PagI	TCATGA	2 cut(s)	508, 1840
PaqCI	CACCTGC	1 cut(s)	2594
PctI	GAATGC	1 cut(s)	391
PdmI	GAANNNNTTC	2 cut(s)	391, 1779
PfeI	GAWTC	3 cut(s)	76, 230, 1999
PfoI	TCCNGGA	3 cut(s)	139, 233, 2351
PinAI	ACCGGT	1 cut(s)	1973
PkrI	GCNGC	3 cut(s)	7, 46, 2080
PleI	GAGTC	4 cut(s)	23, 217, 793, 958
PluTI	GGCGCC	1 cut(s)	2697
PpsI	GAGTC	4 cut(s)	23, 217, 793, 958
PpuMI	RGGWCCY	1 cut(s)	2854
Psp124BI	GAGCTC	2 cut(s)	855, 1933
Psp5II	RGGWCCY	1 cut(s)	2854
Psp6I	CCWGG	5 cut(s)	159, 421, 1828, 2351, 2818
PspGI	CCWGG	5 cut(s)	159, 421, 1828, 2351, 2818
PspPI	GGNCC	7 cut(s)	364, 620, 964, 1295, 1303, 1604, 2854
PspPPI	RGGWCCY	1 cut(s)	2854
PsrI	GAACNNNNNNTAC	4 cut(s)	236, 268, 777, 809
PsuI	RGATCY	6 cut(s)	824, 1513, 1556, 1876, 2185, 2841
RsaI	GTAC	6 cut(s)	180, 245, 292, 911, 1537, 2592
RsaNI	GTAC	6 cut(s)	179, 244, 291, 910, 1536, 2591
RseI	CAYNNNNRTG	3 cut(s)	423, 1206, 2481
SacI	GAGCTC	2 cut(s)	855, 1933
SaqAI	TTAA	8 cut(s)	200, 347, 1029, 1149, 1638, 1752, 2103, 2522
SatI	GCNGC	3 cut(s)	6, 45, 2079
Sau96I	GGNCC	7 cut(s)	364, 620, 964, 1295, 1303, 1604, 2854
ScaI	AGTACT	1 cut(s)	245
SchI	GAGTC	4 cut(s)	23, 218, 793, 959
SduI	GDGCHC	6 cut(s)	357, 719, 837, 855, 1933, 2238
SfaNI	GCATC	3 cut(s)	427, 2380, 2500
SfcI	CTRYAG	3 cut(s)	1683, 2166, 2338
SfoI	GGCGCC	1 cut(s)	2695
SfuI	TTCGAA	1 cut(s)	1455
SgrAI	CRCCGGYG	1 cut(s)	1973
SinI	GGWCC	5 cut(s)	364, 1295, 1303, 1604, 2854
SmiI	ATTTAAAT	1 cut(s)	348
SmiMI	CAYNNNNRTG	3 cut(s)	423, 1206, 2481
SmlI	CTYRAG	1 cut(s)	2429
SmoI	CTYRAG	1 cut(s)	2429
SspDI	GGCGCC	1 cut(s)	2693
SspI	AATATT	1 cut(s)	1769
SspMI	CTAG	2 cut(s)	2042, 2189
SstI	GAGCTC	2 cut(s)	855, 1933
StyI	CCWWGG	4 cut(s)	119, 1552, 2003, 2902
SwaI	ATTTAAAT	1 cut(s)	348
TaiI	ACGT	3 cut(s)	254, 2097, 2650
TaqI	TCGA	5 cut(s)	605, 663, 1260, 1455, 2498
TatI	WGTACW	3 cut(s)	243, 909, 2590
TauI	GCSGC	2 cut(s)	8, 47
TfiI	GAWTC	3 cut(s)	76, 230, 1999
Tru1I	TTAA	8 cut(s)	200, 347, 1029, 1149, 1638, 1752, 2103, 2522
Tru9I	TTAA	8 cut(s)	200, 347, 1029, 1149, 1638, 1752, 2103, 2522
TscAI	CASTG	9 cut(s)	76, 412, 744, 1492, 1509, 2238, 2256, 2427, 2817
TseFI	GTSAC	5 cut(s)	403, 1009, 1208, 2555, 2866
TseI	GCWGC	1 cut(s)	2078
Tsp45I	GTSAC	5 cut(s)	403, 1009, 1208, 2555, 2866
TspDTI	ATGAA	9 cut(s)	1157, 1485, 1626, 1829, 2440, 2711, 2723, 2777, 2909
TspGWI	ACGGA	4 cut(s)	71, 601, 1148, 2471
TspRI	CASTG	9 cut(s)	76, 412, 744, 1492, 1509, 2238, 2256, 2427, 2817
VneI	GTGCAC	1 cut(s)	833
VpaK11BI	GGWCC	5 cut(s)	364, 1295, 1303, 1604, 2854
XagI	CCTNNNNNAGG	1 cut(s)	1728
XapI	RAATTY	8 cut(s)	310, 772, 987, 1144, 1238, 1614, 1740, 2775
XbaI	TCTAGA	2 cut(s)	2041, 2188
XceI	RCATGY	2 cut(s)	794, 2202
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1529, 1791
XmnI	GAANNNNTTC	2 cut(s)	391, 1779
XspI	CTAG	2 cut(s)	2042, 2189
ZrmI	AGTACT	1 cut(s)	245
Zsp2I	ATGCAT	1 cut(s)	2478

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.