AT1G10680

coupled to transmembrane movement of substances

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0009779
Physical Location & Seq
Reverse (-)
3538333 .. 3543925
5593 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G10680.2

Sequence Viewer

Length: 3684 bp
ATGCAACCGTCAAATGATCCAGCAATCGTGGACATGGCAGCGGCGGAGAAGGAGAAGAAGCGGCCAAGTGTATCATTTCTTAAGCTCTTCTCATTTGCTGATTTTTACGATTGCGTTCTGATGGCTCTTGGATCCATTGGTGCTTGCATTCACGGAGCCTCCGTTCCAGTTTTCTTCATCTTCTTTGGCAAACTCATCAACATCATCGGTCTTGCTTACCTCTTCCCACAAGAAGCTTCTCACAAAGTCGCCAAGTACTCGTTGGATTTTGTATATCTGAGTGTGGTGATACTCTTTTCATCATGGTTAGAGGTTGCATGTTGGATGCATACGGGAGAGAGACAAGCAGCGAAGATTAGAAAAGCTTATTTGCGGTCAATGTTAAGCCAAGACATAAGCTTATTCGACACCGAGATTTCCACCGGAGAAGTCATCTCCGCCATCACCTCCGAGATCCTTGTTGTCCAAGACGCTATCTCCGAGAAGGTAGGGAATTTCATGCACTTCATAAGCCGGTTCATAGCCGGTTTTGCGATCGGGTTCGCGAGCGTATGGCAAATAAGTCTCGTCACTCTCTCCATAGTCCCATTCATTGCTCTCGCCGGCGGAATTTACGCTTTTGTCAGCAGCGGACTTATCGTCCGTGTCCGGAAATCATACGTCAAGGCCAACGAGATCGCAGAAGAGGTGATCGGGAATGTGAGGACCGTACAAGCGTTTACGGGAGAAGAAAAGGCGGTGAGTTCGTACCAAGGAGCTTTGAGGAACACTTACAATTACGGGAGAAAAGCCGGTTTAGCCAAAGGACTAGGGCTTGGTTCGTTGCATTTTGTCCTGTTTTTGTCTTGGGCTTTGCTCATTTGGTTCACAAGCATCGTTGTTCACAAGGGAATCGCTAATGGTGGCGAATCTTTCACGACCATGCTCAATGTTGTCATCGCTGGCTTGTCTCTTGGTCAGGCGGCTCCTGACATTTCCACATTCATGCGAGCCAGTGCTGCTGCGTATCCGATTTTTCAGATGATTGAACGGAACACGGAGGATAAAACAGGTCGTAAACTCGGGAATGTAAACGGAGACATTCTATTCAAAGACGTGACTTTCACATACCCCTCTCGCCCTGATGTGGTGATCTTTGACAAGCTAAACTTCGTGATCCCTGCGGGGAAAGTCGTGGCTCTTGTTGGAGGAAGCGGGTCTGGGAAAAGCACCATGATATCCTTAATTGAACGATTCTACGAGCCTACCGATGGAGCGGTGATGCTGGATGGGAACGATATCAGATACTTGGATCTTAAGTGGTTAAGAGGTCATATTGGTTTGGTTAATCAAGAGCCTGTCCTGTTTGCAACCACGATTCGCGAGAACATTATGTATGGTAAAGACGATGCCACCTCTGAGGAAATTACCAATGCTGCAAAGCTCTCGGAAGCCATCTCATTCATCAACAACCTACCGGAAGGATTCGAAACTCAGGTAGGTGAGAGAGGAATTCAGCTATCTGGCGGGCAGAAGCAGAGGATCAGTATTTCTAGGGCGATAGTGAAGAATCCATCGATCCTCTTACTCGATGAGGCAACAAGCGCACTAGATGCAGAGTCAGAGAAAATCGTGCAGGAAGCTTTGGACAGGGTGATGGTTGGGAGAACAACGGTCGTGGTGGCTCATAGACTCTCCACTGTAAGAAACGCTGACATCATAGCCGTTGTTGGAGGAGGGAAGATCATTGAGTCCGGAAGCCACGACGAACTCATATCTAACCCTGATGGAGCTTATTCTTCTCTTCTTCGTATCCAAGAAGCTGCCAGCCCTAACTTGAACCACACTCCAAGCTTACCAGTCAGTACAAAACCCTTGCCGGAACTACCAATCACGGAGACGACCTCGTCCATTCATCAATCAGTAAACCAACCAGATACGACCAAACAGGCCAAAGTAACGGTGGGACGTCTTTACTCTATGATCCGTCCGGATTGGAAATACGGATTATGTGGGACGTTAGGTTCCTTTATTGCGGGATCTCAAATGCCGCTTTTCGCGCTTGGTATCGCACAGGCTTTAGTATCTTATTACATGGACTGGGAGACAACTCAAAACGAGGTCAAAAGAATCTCTATCCTCTTCTGTTGTGGCTCCGTCATCACTGTCATCGTACATACCATTGAGCATACCACCTTTGGTATTATGGGTGAGCGTCTCACTCTCCGAGTCCGACAAAAGATGTTTTCAGCGATCTTGAGGAATGAAATCGGGTGGTTTGATAAGGTTGATAACACTAGTTCGATGTTGGCATCGCGGCTTGAAAGTGATGCGACTTTGCTTAGAACCATCGTGGTCGATAGATCGACAATTCTACTTGAGAATTTAGGTCTTGTTGTGACTGCTTTCATCATTTCTTTTATACTCAACTGGCGTCTCACTCTTGTTGTCTTGGCCACATACCCCTTGATTATAAGCGGTCATATCAGCGAGAAAATTTTCATGCAAGGCTATGGAGGAAACTTGAGTAAAGCGTATTTAAAGGCTAACATGTTGGCTGGAGAGTCTATTAGCAACATCCGAACCGTGGTCGCCTTTTGTGCAGAGGAGAAGGTTTTAGACCTTTATTCCAAAGAGCTTCTAGAACCTTCAGAACGTTCTTTCAGGCGTGGACAGATGGCCGGGATTTTGTACGGTGTTTCTCAATTCTTCATCTTCTCCTCCTACGGCCTTGCCCTCTGGTACGGATCGATTTTGATGGAGAAAGGATTATCAAGTTTCGAATCTGTGATGAAAACATTCATGGTTTTGATAGTAACGGCGTTAGTGATGGGTGAAGTGTTGGCTCTAGCCCCAGATCTTCTTAAAGGAAACCAGATGGTTGTTTCGGTTTTCGAGCTATTGGATCGAAGAACACAGGTTGTTGGTGATACTGGTGAGGAGCTGAGTAATGTGGAAGGCACTATAGAGCTCAAAGGTGTTCATTTCAGTTATCCTTCACGGCCTGATGTGACTATTTTCAGCGATTTCAATCTACTAGTTCCTTCTGGCAAAAGCATGGCGCTTGTGGGACAAAGCGGATCTGGAAAAAGTTCGGTTCTTTCGCTTGTTCTCCGGTTCTACGATCCTACAGCCGGAATAATCATGATAGACGGGCAAGACATCAAGAAACTGAAGTTGAAATCACTGCGAAGACACATTGGTCTGGTTCAACAAGAACCGGCTCTGTTTGCAACGACTATCTATGAGAACATCTTGTATGGTAAAGAAGGAGCTTCTGAATCTGAAGTCATGGAAGCAGCTAAGCTTGCAAATGCTCACAGTTTCATCAGCTCTCTCCCTGAAGGTTACTCAACCAAAGTCGGTGAACGTGGCATTCAGATGTCAGGCGGCCAGAGACAGAGAATTGCTATAGCTAGAGCCGTCCTCAAGAACCCAGAGATTCTGCTTCTTGATGAAGCCACAAGCGCCTTAGATGTTGAATCAGAGCGTGTGGTACAACAAGCATTGGATCGGCTAATGAGAGACCGAACCACGGTGGTGGTGGCTCACCGGCTATCGACGATCAAGAACTCAGATATGATAAGTGTAATACAAGACGGTAAGATCATAGAGCAAGGCAGCCACAATATCCTCGTTGAGAACAAGAATGGTCCCTACTCTAAACTTATCAGTCTTCAGCAGCGGCAGCGCCATCATCCTTAA

Protein Analysis

1227

Amino Acids

134.47

Weight (kDa)

6.76

Isoelectric Point (pI)

36.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane PF00664 44 - 313 1.2e-45 ABC transporter transmembrane region
ABC_tran PF00005 380 - 528 1.7e-32 ABC transporter
ABC_membrane PF00664 664 - 934 3.3e-46 ABC transporter transmembrane region
ABC_tran PF00005 1001 - 1149 1e-32 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0009779)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10680 AT1G10680 AT4G25960
fragaria_vesca FvH4_5g22520 FvH4_5g22520 FvH4_5g22520 FvH4_5g22520 FvH4_5g22520 FvH4_5g22520
malus_domestica MD06G1037400.v1.1
prunus_persica Prupe.5G031600_v2.0.a1
pyrus_communis pycom06g03090
rosa_chinensis RchiOBHm_Chr7g0210291
rosa_laevigata RLG00000003051 RLG00000029346
rosa_multiflora Rmu_sc0003639.1_g000002
rosa_roxburghii Rroxscaffold_3G00248450
rosa_rugosa Rorug07G0120500
rosa_samantha Rh7AG253400 Rh7CG270300
rosa_wichuraiana Rw7G021600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2453
AasI GACNNNNNNGTC 2 cut(s) 1885, 3180
AatII GACGTC 1 cut(s) 1951
AccBSI CCGCTC 1 cut(s) 1256
AccII CGCG 4 cut(s) 545, 1362, 2039, 2296
AccIII TCCGGA 3 cut(s) 648, 1733, 1969
AclI AACGTT 1 cut(s) 2635
AcoI YGGCCR 4 cut(s) 62, 2433, 2658, 3370
AcsI RAATTY 5 cut(s) 493, 609, 1491, 2362, 2475
AcuI CTGAAG 5 cut(s) 2613, 3173, 3285, 3342, 3641
AcyI GRCGYC 2 cut(s) 1948, 2413
AfaI GTAC 8 cut(s) 257, 711, 749, 1846, 2154, 2672, 2723, 3477
AfiI CCNNNNNNNGG 5 cut(s) 1126, 1250, 2566, 3113, 3514
AflII CTTAAG 2 cut(s) 80, 1295
AflIII ACRYGT 1 cut(s) 2529
AgsI TTSAA 9 cut(s) 1028, 1090, 1229, 1819, 2303, 3010, 3160, 3191, 3461
AhdI GACNNNNNGTC 1 cut(s) 638
AhlI ACTAGT 2 cut(s) 2276, 3016
AjiI CACGTC 1 cut(s) 1096
AleI CACNNNNGTG 1 cut(s) 3518
AloI GAACNNNNNNTCC 4 cut(s) 147, 179, 1987, 2019
Alw21I GWGCWC 1 cut(s) 2952
Ama87I CYCGRG 1 cut(s) 1061
Aor13HI TCCGGA 3 cut(s) 648, 1733, 1969
AoxI GGCC 8 cut(s) 62, 666, 1929, 2433, 2658, 2707, 2981, 3370
ApoI RAATTY 5 cut(s) 493, 609, 1491, 2362, 2475
Asp700I GAANNNNTTC 3 cut(s) 2477, 2777, 3070
AspLEI GCGC 5 cut(s) 1586, 2041, 3043, 3449, 3672
AspS9I GGNCC 2 cut(s) 705, 3632
AsuC2I CCSGG 1 cut(s) 2662
AsuII TTCGAA 2 cut(s) 1467, 2760
AvaI CYCGRG 1 cut(s) 1061
AvaII GGWCC 2 cut(s) 705, 3632
BalI TGGCCA 1 cut(s) 2435
BamHI GGATCC 1 cut(s) 131
BanII GRGCYC 1 cut(s) 2952
BbsI GAAGAC 2 cut(s) 3178, 3647
Bbv12I GWGCWC 1 cut(s) 2952
BceAI ACGGC 5 cut(s) 1688, 2722, 2814, 2996, 3386
BcgI CGANNNNNNTGC 2 cut(s) 1407, 1441
BciVI GTATCC 2 cut(s) 1017, 1802
BcnI CCSGG 1 cut(s) 2662
BcuI ACTAGT 2 cut(s) 2276, 3016
BfaI CTAG 8 cut(s) 809, 1533, 1589, 2277, 2621, 2828, 3017, 3396
BfmI CTRYAG 3 cut(s) 2943, 3108, 3390
BfoI RGCGCY 3 cut(s) 3044, 3450, 3673
BfrI CTTAAG 2 cut(s) 80, 1295
BfuI GTATCC 2 cut(s) 1017, 1802
BglII AGATCT 1 cut(s) 2836
BlpI GCTNAGC 1 cut(s) 3282
BmcAI AGTACT 1 cut(s) 257
Bme1390I CCNGG 1 cut(s) 2662
Bme18I GGWCC 2 cut(s) 705, 3632
BmeRI GACNNNNNGTC 1 cut(s) 638
BmeT110I CYCGRG 1 cut(s) 1061
BmgBI CACGTC 1 cut(s) 1096
BmgT120I GGNCC 2 cut(s) 705, 3632
BmiI GGNNCC 6 cut(s) 133, 157, 966, 2005, 2134, 3634
BmrFI CCNGG 1 cut(s) 2662
BmrI ACTGGG 1 cut(s) 2089
BmsI GCATC 7 cut(s) 315, 882, 1251, 1378, 1582, 2299, 2300
BmuI ACTGGG 1 cut(s) 2089
BpiI GAAGAC 2 cut(s) 3178, 3647
BplI GAGNNNNNCTC 4 cut(s) 1868, 1900, 3390, 3422
BpmI CTGGAG 1 cut(s) 2559
Bpu1102I GCTNAGC 1 cut(s) 3282
Bpu14I TTCGAA 2 cut(s) 1467, 2760
BpuEI CTTGAG 4 cut(s) 2257, 2378, 2524, 3392
BpuMI CCSGG 1 cut(s) 2662
Bsa29I ATCGAT 2 cut(s) 1556, 2729
BsaBI GATNNNNATC 1 cut(s) 3009
BsaHI GRCGYC 2 cut(s) 1948, 2413
BsaI GGTCTC 1 cut(s) 3498
BsaJI CCNNGG 3 cut(s) 751, 2565, 3513
BsaWI WCCGGW 6 cut(s) 422, 648, 1456, 1733, 1969, 3093
BsaXI ACNNNNNCTCC 6 cut(s) 143, 147, 173, 177, 461, 491
Bsc4I CCNNNNNNNGG 5 cut(s) 1126, 1250, 2566, 3113, 3514
Bse118I RCCGGY 6 cut(s) 513, 524, 602, 791, 3199, 3531
Bse1I ACTGG 6 cut(s) 167, 993, 1838, 2084, 2414, 2917
Bse3DI GCAATG 1 cut(s) 591
Bse8I GATNNNNATC 1 cut(s) 3009
BseAI TCCGGA 3 cut(s) 648, 1733, 1969
BseCI ATCGAT 2 cut(s) 1556, 2729
BseDI CCNNGG 3 cut(s) 751, 2565, 3513
BseGI GGATG 4 cut(s) 330, 1273, 2556, 3676
BseJI GATNNNNATC 1 cut(s) 3009
BseLI CCNNNNNNNGG 5 cut(s) 1126, 1250, 2566, 3113, 3514
BseMI GCAATG 1 cut(s) 591
BseMII CTCAG 5 cut(s) 269, 1389, 1487, 2915, 3567
BseNI ACTGG 6 cut(s) 167, 993, 1838, 2084, 2414, 2917
BseRI GAGGAG 4 cut(s) 1728, 2600, 2689, 2933
BsgI GTGCAG 2 cut(s) 1634, 2601
Bsh1236I CGCG 4 cut(s) 545, 1362, 2039, 2296
Bsh1285I CGRYCG 2 cut(s) 537, 1656
BshFI GGCC 8 cut(s) 64, 668, 1931, 2435, 2660, 2709, 2983, 3372
BshVI ATCGAT 2 cut(s) 1556, 2729
BsiEI CGRYCG 2 cut(s) 537, 1656
BsiHKAI GWGCWC 1 cut(s) 2952
BsiHKCI CYCGRG 1 cut(s) 1061
BslFI GGGAC 5 cut(s) 569, 1959, 2008, 3063, 3618
BslI CCNNNNNNNGG 5 cut(s) 1126, 1250, 2566, 3113, 3514
BsmBI CGTCTC 3 cut(s) 1871, 2201, 2420
BsmFI GGGAC 5 cut(s) 569, 1959, 2008, 3063, 3618
BsmI GAATGC 2 cut(s) 147, 3354
BsnI GGCC 8 cut(s) 64, 668, 1931, 2435, 2660, 2709, 2983, 3372
Bso31I GGTCTC 1 cut(s) 3498
BsoBI CYCGRG 1 cut(s) 1061
Bsp119I TTCGAA 2 cut(s) 1467, 2760
Bsp1286I GDGCHC 1 cut(s) 2952
Bsp13I TCCGGA 3 cut(s) 648, 1733, 1969
Bsp1720I GCTNAGC 1 cut(s) 3282
Bsp68I TCGCGA 2 cut(s) 545, 1362
BspANI GGCC 8 cut(s) 64, 668, 1931, 2435, 2660, 2709, 2983, 3372
BspCNI CTCAG 5 cut(s) 270, 1390, 1486, 2916, 3566
BspDI ATCGAT 2 cut(s) 1556, 2729
BspEI TCCGGA 3 cut(s) 648, 1733, 1969
BspFNI CGCG 4 cut(s) 545, 1362, 2039, 2296
BspHI TCATGA 1 cut(s) 3123
BspLI GGNNCC 6 cut(s) 133, 157, 966, 2005, 2134, 3634
BspQI GCTCTTC 1 cut(s) 92
BspT104I TTCGAA 2 cut(s) 1467, 2760
BspTI CTTAAG 2 cut(s) 80, 1295
BspTNI GGTCTC 1 cut(s) 3498
BsrBI CCGCTC 1 cut(s) 1256
BsrDI GCAATG 1 cut(s) 591
BsrFI RCCGGY 6 cut(s) 513, 524, 602, 791, 3199, 3531
BsrI ACTGG 6 cut(s) 167, 993, 1838, 2084, 2414, 2917
BssAI RCCGGY 6 cut(s) 513, 524, 602, 791, 3199, 3531
BssECI CCNNGG 3 cut(s) 751, 2565, 3513
BssNI GRCGYC 2 cut(s) 1948, 2413
BssT1I CCWWGG 1 cut(s) 751
Bst6I CTCTTC 5 cut(s) 92, 227, 678, 1788, 2126
BstACI GRCGYC 2 cut(s) 1948, 2413
BstAFI CTTAAG 2 cut(s) 80, 1295
BstAPI GCANNNNNTGC 1 cut(s) 1592
BstBI TTCGAA 2 cut(s) 1467, 2760
BstC8I GCNNGC 8 cut(s) 145, 547, 604, 943, 990, 1508, 1807, 3288
BstDEI CTNAG 8 cut(s) 278, 1398, 1473, 2321, 2924, 3282, 3451, 3553
BstDSI CCRYGG 2 cut(s) 2565, 3513
BstF5I GGATG 4 cut(s) 330, 1273, 2556, 3676
BstFNI CGCG 4 cut(s) 545, 1362, 2039, 2296
BstH2I RGCGCY 3 cut(s) 3044, 3450, 3673
BstHHI GCGC 5 cut(s) 1586, 2041, 3043, 3449, 3672
BstMCI CGRYCG 2 cut(s) 537, 1656
BstNSI RCATGY 2 cut(s) 321, 2533
BstSCI CCNGG 1 cut(s) 2660
BstSFI CTRYAG 3 cut(s) 2943, 3108, 3390
BstUI CGCG 4 cut(s) 545, 1362, 2039, 2296
BstV2I GAAGAC 2 cut(s) 3178, 3647
BstX2I RGATCY 6 cut(s) 131, 453, 1291, 2018, 2836, 3059
BstXI CCANNNNNNTGG 1 cut(s) 3520
BstYI RGATCY 6 cut(s) 131, 453, 1291, 2018, 2836, 3059
Bsu15I ATCGAT 2 cut(s) 1556, 2729
BsuI GTATCC 2 cut(s) 1017, 1802
BsuRI GGCC 8 cut(s) 64, 668, 1931, 2435, 2660, 2709, 2983, 3372
BsuTUI ATCGAT 2 cut(s) 1556, 2729
BtgI CCRYGG 2 cut(s) 2565, 3513
BtgZI GCGATG 2 cut(s) 922, 2277
BtrI CACGTC 1 cut(s) 1096
BtsCI GGATG 4 cut(s) 330, 1273, 2556, 3676
BtsI GCAGTG 1 cut(s) 3164
BtsIMutI CAGTG 4 cut(s) 1000, 1677, 2142, 3164
BtuMI TCGCGA 2 cut(s) 545, 1362
Cac8I GCNNGC 8 cut(s) 145, 547, 604, 943, 990, 1508, 1807, 3288
CciI TCATGA 1 cut(s) 3123
CfoI GCGC 5 cut(s) 1586, 2041, 3043, 3449, 3672
Cfr10I RCCGGY 6 cut(s) 513, 524, 602, 791, 3199, 3531
Cfr13I GGNCC 2 cut(s) 705, 3632
ClaI ATCGAT 2 cut(s) 1556, 2729
CseI GACGC 3 cut(s) 479, 2183, 2402
Csp6I GTAC 8 cut(s) 256, 710, 748, 1845, 2153, 2671, 2722, 3476
CspCI CAANNNNNGTGG 2 cut(s) 1638, 1673
CviQI GTAC 8 cut(s) 256, 710, 748, 1845, 2153, 2671, 2722, 3476
DdeI CTNAG 8 cut(s) 278, 1398, 1473, 2321, 2924, 3282, 3451, 3553
DraI TTTAAA 1 cut(s) 2520
DrdI GACNNNNNNGTC 2 cut(s) 1885, 3180
DriI GACNNNNNGTC 1 cut(s) 638
DseDI GACNNNNNNGTC 2 cut(s) 1885, 3180
EaeI YGGCCR 4 cut(s) 62, 2433, 2658, 3370
Eam1104I CTCTTC 5 cut(s) 92, 227, 678, 1788, 2126
Eam1105I GACNNNNNGTC 1 cut(s) 638
EarI CTCTTC 5 cut(s) 92, 227, 678, 1788, 2126
EciI GGCGGA 3 cut(s) 59, 427, 621
Ecl136II GAGCTC 1 cut(s) 2950
Eco130I CCWWGG 1 cut(s) 751
Eco24I GRGCYC 1 cut(s) 2952
Eco31I GGTCTC 1 cut(s) 3498
Eco32I GATATC 2 cut(s) 1218, 1279
Eco47I GGWCC 2 cut(s) 705, 3632
Eco53kI GAGCTC 1 cut(s) 2950
Eco57I CTGAAG 5 cut(s) 2613, 3173, 3285, 3342, 3641
Eco88I CYCGRG 1 cut(s) 1061
EcoICRI GAGCTC 1 cut(s) 2950
EcoRI GAATTC 1 cut(s) 1491
EcoRV GATATC 2 cut(s) 1218, 1279
EcoT14I CCWWGG 1 cut(s) 751
EcoT22I ATGCAT 1 cut(s) 330
EcoT38I GRGCYC 1 cut(s) 2952
ErhI CCWWGG 1 cut(s) 751
Esp3I CGTCTC 3 cut(s) 1871, 2201, 2420
FalI AAGNNNNNCTT 2 cut(s) 1133, 1165
FaqI GGGAC 5 cut(s) 569, 1959, 2008, 3063, 3618
FauI CCCGC 4 cut(s) 1156, 1187, 1499, 2008
FokI GGATG 4 cut(s) 337, 1280, 2543, 3663
FriOI GRGCYC 1 cut(s) 2952
FspBI CTAG 8 cut(s) 809, 1533, 1589, 2277, 2621, 2828, 3017, 3396
GlaI GCGC 5 cut(s) 1585, 2040, 3042, 3448, 3671
GsuI CTGGAG 1 cut(s) 2559
HaeII RGCGCY 3 cut(s) 3044, 3450, 3673
HaeIII GGCC 8 cut(s) 64, 668, 1931, 2435, 2660, 2709, 2983, 3372
HgaI GACGC 3 cut(s) 479, 2183, 2402
HhaI GCGC 5 cut(s) 1586, 2041, 3043, 3449, 3672
Hin1I GRCGYC 2 cut(s) 1948, 2413
Hin6I GCGC 5 cut(s) 1584, 2039, 3041, 3447, 3670
HinP1I GCGC 5 cut(s) 1584, 2039, 3041, 3447, 3670
HindIII AAGCTT 6 cut(s) 234, 363, 397, 1620, 1831, 3284
Hpy166II GTNNAC 9 cut(s) 31, 720, 867, 883, 1058, 1072, 1906, 2651, 3347
Hpy8I GTNNAC 9 cut(s) 31, 720, 867, 883, 1058, 1072, 1906, 2651, 3347
Hpy99I CGWCG 2 cut(s) 1748, 3544
HpyCH4IV ACGT 6 cut(s) 660, 1095, 1948, 1997, 2635, 3349
HpyF3I CTNAG 8 cut(s) 278, 1398, 1473, 2321, 2924, 3282, 3451, 3553
HpySE526I ACGT 6 cut(s) 660, 1095, 1948, 1997, 2635, 3349
Hsp92I GRCGYC 2 cut(s) 1948, 2413
HspAI GCGC 5 cut(s) 1584, 2039, 3041, 3447, 3670
Kpn2I TCCGGA 3 cut(s) 648, 1733, 1969
KroI GCCGGC 1 cut(s) 602
KroNI GCCGGC 1 cut(s) 604
LguI GCTCTTC 1 cut(s) 92
LmnI GCTCC 8 cut(s) 155, 755, 970, 1253, 1769, 2138, 2920, 3251
LweI GCATC 7 cut(s) 315, 882, 1251, 1378, 1582, 2299, 2300
MaeI CTAG 8 cut(s) 809, 1533, 1589, 2277, 2621, 2828, 3017, 3396
MaeII ACGT 6 cut(s) 660, 1095, 1948, 1997, 2635, 3349
MaeIII GTNAC 7 cut(s) 568, 1096, 1936, 2377, 2794, 2989, 3326
MbiI CCGCTC 1 cut(s) 1256
MflI RGATCY 6 cut(s) 131, 453, 1291, 2018, 2836, 3059
MhlI GDGCHC 1 cut(s) 2952
MlsI TGGCCA 1 cut(s) 2435
MluNI TGGCCA 1 cut(s) 2435
MlyI GAGTC 5 cut(s) 1607, 1665, 1739, 2217, 2552
MmeI TCCRAC 5 cut(s) 243, 302, 1165, 1690, 2236
Mox20I TGGCCA 1 cut(s) 2435
Mph1103I ATGCAT 1 cut(s) 330
MreI CGCCGGCG 1 cut(s) 602
MroI TCCGGA 3 cut(s) 648, 1733, 1969
MroNI GCCGGC 1 cut(s) 602
MroXI GAANNNNTTC 3 cut(s) 2477, 2777, 3070
MscI TGGCCA 1 cut(s) 2435
MseI TTAA 9 cut(s) 81, 383, 1223, 1296, 1304, 1326, 2519, 2844, 3682
MslI CAYNNNNRTG 4 cut(s) 920, 983, 3359, 3518
Msp20I TGGCCA 1 cut(s) 2435
MspA1I CMGCKG 3 cut(s) 41, 630, 3664
MspCI CTTAAG 2 cut(s) 80, 1295
MspR9I CCNGG 1 cut(s) 2662
Mva1269I GAATGC 2 cut(s) 147, 3354
MvnI CGCG 4 cut(s) 545, 1362, 2039, 2296
NaeI GCCGGC 1 cut(s) 604
NciI CCSGG 1 cut(s) 2662
NgoMIV GCCGGC 1 cut(s) 602
NlaIV GGNNCC 6 cut(s) 133, 157, 966, 2005, 2134, 3634
NmuCI GTSAC 4 cut(s) 568, 1096, 2377, 2989
NruI TCGCGA 2 cut(s) 545, 1362
NsiI ATGCAT 1 cut(s) 330
NspI RCATGY 2 cut(s) 321, 2533
NspV TTCGAA 2 cut(s) 1467, 2760
OliI CACNNNNGTG 1 cut(s) 3518
PagI TCATGA 1 cut(s) 3123
PciI ACATGT 1 cut(s) 2529
PciSI GCTCTTC 1 cut(s) 92
PcsI WCGNNNNNNNCGW 3 cut(s) 159, 477, 1245
PctI GAATGC 2 cut(s) 147, 3354
PdiI GCCGGC 1 cut(s) 604
PdmI GAANNNNTTC 3 cut(s) 2477, 2777, 3070
PflFI GACNNNGTC 1 cut(s) 1885
Ple19I CGATCG 1 cut(s) 537
PleI GAGTC 5 cut(s) 1606, 1665, 1738, 2216, 2551
PpsI GAGTC 5 cut(s) 1606, 1665, 1738, 2216, 2551
PscI ACATGT 1 cut(s) 2529
PsiI TTATAA 1 cut(s) 2453
Psp124BI GAGCTC 1 cut(s) 2952
Psp1406I AACGTT 1 cut(s) 2635
PspN4I GGNNCC 6 cut(s) 133, 157, 966, 2005, 2134, 3634
PspPI GGNCC 2 cut(s) 705, 3632
PsuI RGATCY 6 cut(s) 131, 453, 1291, 2018, 2836, 3059
PsyI GACNNNGTC 1 cut(s) 1885
PvuI CGATCG 1 cut(s) 537
RruI TCGCGA 2 cut(s) 545, 1362
RsaI GTAC 8 cut(s) 257, 711, 749, 1846, 2154, 2672, 2723, 3477
RsaNI GTAC 8 cut(s) 256, 710, 748, 1845, 2153, 2671, 2722, 3476
RseI CAYNNNNRTG 4 cut(s) 920, 983, 3359, 3518
SacI GAGCTC 1 cut(s) 2952
SapI GCTCTTC 1 cut(s) 92
SaqAI TTAA 9 cut(s) 81, 383, 1223, 1296, 1304, 1326, 2519, 2844, 3682
Sau96I GGNCC 2 cut(s) 705, 3632
ScaI AGTACT 1 cut(s) 257
SchI GAGTC 5 cut(s) 1607, 1665, 1739, 2217, 2552
ScrFI CCNGG 1 cut(s) 2662
SduI GDGCHC 1 cut(s) 2952
SfaNI GCATC 7 cut(s) 315, 882, 1251, 1378, 1582, 2299, 2300
SfcI CTRYAG 3 cut(s) 2943, 3108, 3390
SfuI TTCGAA 2 cut(s) 1467, 2760
SgrAI CRCCGGYG 1 cut(s) 602
SinI GGWCC 2 cut(s) 705, 3632
SmiMI CAYNNNNRTG 4 cut(s) 920, 983, 3359, 3518
SmlI CTYRAG 6 cut(s) 80, 1295, 2236, 2357, 2503, 3407
SmoI CTYRAG 6 cut(s) 80, 1295, 2236, 2357, 2503, 3407
SpeI ACTAGT 2 cut(s) 2276, 3016
SspMI CTAG 8 cut(s) 809, 1533, 1589, 2277, 2621, 2828, 3017, 3396
SstI GAGCTC 1 cut(s) 2952
StyD4I CCNGG 1 cut(s) 2660
StyI CCWWGG 1 cut(s) 751
TaiI ACGT 6 cut(s) 663, 1098, 1951, 2000, 2638, 3352
TaqII GACCGA 2 cut(s) 197, 3522
TatI WGTACW 2 cut(s) 255, 1844
TauI GCSGC 7 cut(s) 44, 64, 965, 2032, 2299, 3372, 3667
Tru1I TTAA 9 cut(s) 81, 383, 1223, 1296, 1304, 1326, 2519, 2844, 3682
Tru9I TTAA 9 cut(s) 81, 383, 1223, 1296, 1304, 1326, 2519, 2844, 3682
TscAI CASTG 4 cut(s) 1000, 1684, 2149, 3171
TseFI GTSAC 4 cut(s) 568, 1096, 2377, 2989
Tsp45I GTSAC 4 cut(s) 568, 1096, 2377, 2989
TspRI CASTG 4 cut(s) 1000, 1684, 2149, 3171
Tth111I GACNNNGTC 1 cut(s) 1885
Vha464I CTTAAG 2 cut(s) 80, 1295
VpaK11BI GGWCC 2 cut(s) 705, 3632
XapI RAATTY 5 cut(s) 493, 609, 1491, 2362, 2475
XbaI TCTAGA 1 cut(s) 2620
XceI RCATGY 2 cut(s) 321, 2533
XcmI CCANNNNNNNNNTGG 3 cut(s) 259, 1939, 3520
XmnI GAANNNNTTC 3 cut(s) 2477, 2777, 3070
XspI CTAG 8 cut(s) 809, 1533, 1589, 2277, 2621, 2828, 3017, 3396
ZraI GACGTC 1 cut(s) 1949
ZrmI AGTACT 1 cut(s) 257
Zsp2I ATGCAT 1 cut(s) 330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.