Rw7G021600

ABC transporter B family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0009779
Physical Location & Seq
Reverse (-)
25087717 .. 25095691
7975 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G021600.1

Sequence Viewer

Length: 3723 bp
ATGTTGAAGTTGAAGAAGAAGAAGGAGAAACAAGAAGAAGAGAAAGAGAACAAGAAGCGGAACGTTCCATTACTGAAGCTCTTCTCTTTCGCTGATTCGTACGACTGCCTTTTGATGGCAATCGGATCTATCGGGGCCTGTGTTCATGGCGCTTCAGTCCCTGTCTTTTTTATTTTCTTCGGAAAAATGATAAACATTATTGGCCTTGCTTATCTCTTTCCCAAAGAAGCTTCCTCTAAAGTAGCCAAGTACTCCTTGGATTTTGTATATCTCAGTGTAGCTATATTGTTTTCATCCTGGACCGAGGTGGCTTGTTGGATGCACACTGGGGAAAGACAAGCAGCGAAAATGAGGATGGCATATTTGAGAGCCATGCTGAATCAAGATATAAGTCTTTTCGACACTGAAGCTTCTACTGGGCAAGTCATTTCTGCAATTACAAGTGACATTATTGTTGTTCAAGATGCTCTTTCTGAGAAGGTGGGGAACTTCATGCACTACATAAGCAGATTTCTTGCAGGATTCACAATTGGGTTTGTTAGGGTTTGGCAAATTAGTCTGGTCACTCTCTCCATTGTACCCCTGATTGCCCTTGCTGGCGGTATCTATGCCTTTGTTACTATTGGCCTCATTGCTAGAGTCAGAAAGTCCTATATCAAGGCTGGGGAAATTGCTGAAGAGGTGATAGGAAATGTAAGGACAGTGCAAGCATTTGCAGCAGAAGAAAGGGCTGTAAGGCAATACAAGACAGCTCTGTTGGGTACATACAAGTATGGGAAGAAAGCAGGACTGGCCAAGGGTTTGGGGCTGGGCTCCCTGCACTGCACCCTTTTTCTTTCATGGGCATTACTCGTTTGGTTCACTAGCGTTGTTGTGCACAAGAATATCGCTAATGGTGGCGAGTCTTTCACCACTATGCTTAATGTTGTTATTGCTGGCCTGTCACTAGGGCAAGCAGCGCCTGACATCTCTGCATTCATACGAGCCAAGGCAGCTGCGTATCCCATATTTGAGATGATAGAGAGGAACACAGTGAACCAATCCAGTAGCAAAACTGGCCGGAAACTGGACAAACTTGAGGGGCAGATCCAATTCAAGGATGTATCTTTTAGCTACCCATCTCGTATAGATGTTAGTATCTTCAATAAGCTCAATCTTGATATCCCTGCTGGGAAGATTGTAGCCCTTGTGGGAGGAAGTGGTTCTGGCAAGAGTACAGTTATATCTTTGATTGAACGATTCTATGAGCCACTTGCCGGCCAGGTGTTGTTAGATGGAAATAATATAAGCGAACTTGACCTCAAATGGATGAGGCAACAAATTGGGCTGGTCAATCAGGAGCCTGCTCTTTTTGCAACAAGCATTAGGGAAAATATTCTTTATGGAAAAGGTGATGCTTCAATGGATGATATCAAACATGCAGCTAAACTTTCAGAGGCTCTTTCTTTCATAAATAACCTCCCAGAGAGATTTGAAACTCAGGTTGGTGAGAGAGGCATACAACTCTCGGGAGGACAAAAGCAAAGGATTGCAATAGCACGTGCAATAGTGAAGAATCCATCAATTCTTCTATTGGATGAAGCAACGAGTGCACTGGATGCAGAGTCTGAGAAGAGTGTGCAGGAGGCACTTGATCGTGCCATGGTAGGGCGAACGACAGTAGTGGTGGCACATCGTCTGTCCACTGTCAGGAATGCAGATCTTATTGCTGTTGTTCAAGAGGGGAAGATAGTTGAAACTGGGAGCCATGAGGAGCTAATTTCAAACCCCAACAGTGTCTATGCAGCACTTGTTCATCTACAAGAGACAGCTTCTTTGCAACGCCACCCCTCATTTGGTCCTAACTTAGGACAGCCACTAAGCATAAGGTATTCACGGGAGTTATCTCGTACTACAGCAAGTTTTGGTGCCAGCTTTCGCTCTGATAAAGAATCTCTTGGCCGTCCTGGTGGTGATGGAACAGAGATTAAGTCAAGATATGTTTCGGCTAGTAGACTGTATTCCATGATCCGTCCAGACTGGCACTATGGGGTGATTGGTACCATTGGTGCCCTTATTGCTGGAGCCCAGATGCCTCTTTTTGCTCTCGGGGTCTCTCAGGCTCTAGTTTCCTATTACATGGACTGGGAAACAACATGCCGTGAGGTTAAAAAGATCTCTTTACTCTTCTGTGGGGCTGCAGTGCTAACTGTCATTGTTCATGCTGTAGCTCATTTTTTTTTTGGAACTATGGGGGAGCGACTCACTCTTCGAGTTAGAGAGAAGATGTTTTCTGCAATTTTGAGGAATGAGATCGGATGGTTTGATGATACAAACAACACCAGTTCTATGCTTTCATCGCGTCTAGAAAGTGATGCAACTTTATTACGAACTATTGTTGTTGATCGCTCAACAATTCTTATACAGAATGTGGGTTTGATAGTTGCCTCATTTATCATTGCCTTCATCTTGAACTGGAGAATCACATTAGTGGTCTTAGCCACGTATCCTTTGATCATTAGTGGTCACATTAGCGAGAAACTTTTCATGAAAGGCTATGGTGGCAACTTGAGCACAGCATATCTGAAAGCTAACATGCTAGCTGGGGAAGCTGTAAGTAACATCCGCACTGTTGCTGCATTTTGTTCTGAGGAGAAGGTCATCGACCTATATGCTCATGAGCTTGTTGAGCCTTCTAGACGTTCATTTACTCGTGGTCAGATTGCTGGCATATTCTATGGTGTATCTCAGTTCTTCATATTCTCATCCTATGGCCTGGCCTTGTGGTATGGTTCTGTTTTGATGGAAAAGGAGCTTGCTACCTTTAAATCTGTCATGAAATCATTCTTTGTTTTGATTGTAACGGCATTAGCAATGGGTGAGACTTTAGCACTTGCCCCAGATCTTTTAAAAGGGAACCAAATGGTGGCATCAGTCTTTGATGTAACAGATCGTAGAACAGAAGTCCTAGGGGACATAGGAGAAGAGGTTACGAAAGTGGAAGGGGCAATTGAGTTAAGGGGTGTTCAATTTAGCTACCCATCGAGACCAGACGTAGTGCTTTTCAGGGACTTCAATTTGAAAGTTCATTCAGGCAAAACTATGGCATTGGTGGGGCAAAGTGGTTCTGGTAAAAGCTCTGTGATTTCTCTAATTCTCAGATTTTATGATCCGACAGCTGGGAAAGTTATGGTAGATGGGAAAGACATCAAGAAACTCAATCTCAAATCTCTTCGAAGACACATTGGCCTGGTTCAACAAGAACCAGCTCTCTTTGCCACATCAATTTATGAAAACATCCTTTATGGAAAAGAAGGAGCTTCTGAAGCAGAGGTAATTGAAGCGGCCAAGCTGGCCAATGCACACAGTTTCATTAGTGCCCTTCCTGAGGGCTACTCCACTAAAGTTGGGGAGCGAGGGGTGCAACTATCTGGTGGCCAAAGGCAAAGGGTGGCCATTGCCCGAGCAGTTCTAAAGAACCCTGAAATTTTGTTACTAGATGAGGCAACCAGCGCTCTAGATGTGGAGTCTGAGCGTGTGGTGCAACAAGCTCTTGATAGATTGATGAAGACCCGTACAACGATAATGGTGGCACATAGGCTGTCCACAATAAAAAATGCAGATGAAATTTCAGTCATACAAGATGGGAAGATTGTAGAGCAAGGAAGTCATTCAACTCTTATAGAGAACAGAAATGGAGCATATTACAAGTTAATAAACATTCAGCAGCAGCAGCAGCATTGA

Protein Analysis

1240

Amino Acids

136.12

Weight (kDa)

9.07

Isoelectric Point (pI)

35.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane PF00664 41 - 311 3.8e-44 ABC transporter transmembrane region
ABC_tran PF00005 382 - 530 2.3e-34 ABC transporter
ABC_membrane PF00664 679 - 949 1.4e-46 ABC transporter transmembrane region
ABC_tran PF00005 1016 - 1164 1.6e-34 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0009779)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10680 AT1G10680 AT4G25960
fragaria_vesca FvH4_5g22520 FvH4_5g22520 FvH4_5g22520 FvH4_5g22520 FvH4_5g22520 FvH4_5g22520
malus_domestica MD06G1037400.v1.1
prunus_persica Prupe.5G031600_v2.0.a1
pyrus_communis pycom06g03090
rosa_chinensis RchiOBHm_Chr7g0210291
rosa_laevigata RLG00000003051 RLG00000029346
rosa_multiflora Rmu_sc0003639.1_g000002
rosa_roxburghii Rroxscaffold_3G00248450
rosa_rugosa Rorug07G0120500
rosa_samantha Rh7AG253400 Rh7CG270300
rosa_wichuraiana Rw7G021600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 2039
AccB1I GGYRCC 3 cut(s) 1907, 2039, 2048
AccB7I CCANNNNNTGG 1 cut(s) 2905
AccI GTMKAC 1 cut(s) 1993
AccII CGCG 1 cut(s) 2341
AciI CCGC 4 cut(s) 58, 600, 2605, 3323
AclI AACGTT 1 cut(s) 63
AclWI GGATC 4 cut(s) 133, 1081, 2002, 3143
AcoI YGGCCR 8 cut(s) 792, 1057, 1258, 1939, 3324, 3333, 3415, 3432
AcsI RAATTY 2 cut(s) 3465, 3606
AcuI CTGAAG 5 cut(s) 95, 138, 426, 696, 3324
AcvI CACGTG 1 cut(s) 1541
AfaI GTAC 8 cut(s) 101, 251, 579, 763, 1216, 1891, 2041, 3556
AfeI AGCGCT 1 cut(s) 3493
AjnI CCWGG 5 cut(s) 296, 1260, 1945, 2754, 3228
AjuI GAANNNNNNNTTGG 2 cut(s) 1467, 1499
AleI CACNNNNGTG 1 cut(s) 2468
Alw21I GWGCWC 3 cut(s) 879, 1594, 2555
Alw26I GTCTC 4 cut(s) 1799, 2098, 2855, 3019
Alw44I GTGCAC 2 cut(s) 875, 1590
AlwI GGATC 4 cut(s) 133, 1081, 2002, 3143
AlwNI CAGNNNCTG 3 cut(s) 161, 962, 1607
Ama87I CYCGRG 3 cut(s) 1507, 2087, 3441
Aor51HI AGCGCT 1 cut(s) 3493
ApaLI GTGCAC 2 cut(s) 875, 1590
ApoI RAATTY 2 cut(s) 3465, 3606
ArsI GACNNNNNNTTYG 4 cut(s) 1798, 1830, 2232, 2264
Asp700I GAANNNNTTC 6 cut(s) 80, 1201, 1374, 2522, 2822, 3649
Asp718I GGTACC 1 cut(s) 2039
AspA2I CCTAGG 1 cut(s) 2947
AspLEI GCGC 3 cut(s) 152, 961, 3494
AspS9I GGNCC 3 cut(s) 135, 300, 1838
AsuHPI GGTGA 7 cut(s) 694, 901, 1403, 1499, 1964, 2044, 2870
AsuII TTCGAA 1 cut(s) 3214
AsuNHI GCTAGC 1 cut(s) 2578
AvaI CYCGRG 3 cut(s) 1507, 2087, 3441
AvaII GGWCC 2 cut(s) 300, 1838
AvrII CCTAGG 1 cut(s) 2947
AxyI CCTNAGG 1 cut(s) 3366
BaeGI GKGCMC 4 cut(s) 879, 1594, 2053, 3361
BalI TGGCCA 4 cut(s) 794, 3335, 3417, 3434
BanI GGYRCC 3 cut(s) 1907, 2039, 2048
BanII GRGCYC 2 cut(s) 815, 2068
BauI CACGAG 1 cut(s) 2691
BbrPI CACGTG 1 cut(s) 1541
BbsI GAAGAC 2 cut(s) 3223, 3554
Bbv12I GWGCWC 3 cut(s) 879, 1594, 2555
BceAI ACGGC 3 cut(s) 1926, 2124, 2859
BcgI CGANNNNNNTGC 2 cut(s) 2633, 2667
BciT130I CCWGG 5 cut(s) 298, 1262, 1947, 2756, 3230
BciVI GTATCC 2 cut(s) 1011, 2496
BclI TGATCA 1 cut(s) 2493
BcoDI GTCTC 4 cut(s) 1799, 2098, 2855, 3019
BfmI CTRYAG 3 cut(s) 1893, 2178, 2205
BfoI RGCGCY 3 cut(s) 153, 962, 3495
BfuI GTATCC 2 cut(s) 1011, 2496
BglI GCCNNNNNGGC 1 cut(s) 3332
BglII AGATCT 3 cut(s) 1699, 2154, 2881
BlnI CCTAGG 1 cut(s) 2947
BmcAI AGTACT 1 cut(s) 251
Bme1390I CCNGG 5 cut(s) 298, 1262, 1947, 2756, 3230
Bme18I GGWCC 2 cut(s) 300, 1838
BmeT110I CYCGRG 3 cut(s) 1507, 2087, 3441
BmgT120I GGNCC 3 cut(s) 135, 300, 1838
BmiI GGNNCC 9 cut(s) 136, 814, 1341, 1745, 1909, 2041, 2050, 2065, 2897
BmrFI CCNGG 5 cut(s) 298, 1262, 1947, 2756, 3230
BmrI ACTGGG 4 cut(s) 336, 426, 1749, 2134
BmsI GCATC 7 cut(s) 309, 454, 1384, 1588, 2061, 2344, 2918
BmtI GCTAGC 1 cut(s) 2582
BmuI ACTGGG 4 cut(s) 336, 426, 1749, 2134
BpiI GAAGAC 2 cut(s) 3223, 3554
BplI GAGNNNNNCTC 2 cut(s) 3359, 3391
BpmI CTGGAG 2 cut(s) 2082, 2476
Bpu14I TTCGAA 1 cut(s) 3214
BpuEI CTTGAG 2 cut(s) 1097, 2569
BsaAI YACGTR 2 cut(s) 1541, 2484
BsaBI GATNNNNATC 1 cut(s) 119
BsaI GGTCTC 2 cut(s) 2098, 3019
BsaJI CCNNGG 6 cut(s) 255, 303, 795, 987, 1641, 2947
BsaXI ACNNNNNCTCC 2 cut(s) 2952, 2982
Bse118I RCCGGY 1 cut(s) 1256
Bse21I CCTNAGG 1 cut(s) 3366
Bse3DI GCAATG 4 cut(s) 630, 2436, 2859, 3435
Bse8I GATNNNNATC 1 cut(s) 119
BseBI CCWGG 5 cut(s) 298, 1262, 1947, 2756, 3230
BseDI CCNNGG 6 cut(s) 255, 303, 795, 987, 1641, 2947
BseJI GATNNNNATC 1 cut(s) 119
BseMI GCAATG 4 cut(s) 630, 2436, 2859, 3435
BseRI GAGGAG 2 cut(s) 1766, 2645
BseSI GKGCMC 4 cut(s) 879, 1594, 2053, 3361
BseYI CCCAGC 5 cut(s) 662, 808, 1169, 2582, 3158
BsgI GTGCAG 3 cut(s) 803, 808, 1640
Bsh1236I CGCG 1 cut(s) 2341
BshNI GGYRCC 3 cut(s) 1907, 2039, 2048
BsiHKAI GWGCWC 3 cut(s) 879, 1594, 2555
BsiHKCI CYCGRG 3 cut(s) 1507, 2087, 3441
BsiSI CCGG 2 cut(s) 1060, 1257
BsiWI CGTACG 1 cut(s) 99
BslFI GGGAC 3 cut(s) 143, 2966, 3062
BsmAI GTCTC 4 cut(s) 1799, 2098, 2855, 3019
BsmFI GGGAC 3 cut(s) 143, 2966, 3062
BsmI GAATGC 2 cut(s) 974, 1699
Bso31I GGTCTC 2 cut(s) 2098, 3019
BsoBI CYCGRG 3 cut(s) 1507, 2087, 3441
Bsp119I TTCGAA 1 cut(s) 3214
Bsp1286I GDGCHC 7 cut(s) 815, 879, 1594, 2053, 2068, 2555, 3361
Bsp19I CCATGG 1 cut(s) 1641
BspACI CCGC 4 cut(s) 58, 600, 2605, 3323
BspFNI CGCG 1 cut(s) 2341
BspHI TCATGA 3 cut(s) 2526, 2656, 2814
BspLI GGNNCC 9 cut(s) 136, 814, 1341, 1745, 1909, 2041, 2050, 2065, 2897
BspMAI CTGCAG 1 cut(s) 2182
BspOI GCTAGC 1 cut(s) 2582
BspPI GGATC 4 cut(s) 133, 1081, 2002, 3143
BspQI GCTCTTC 1 cut(s) 86
BspT104I TTCGAA 1 cut(s) 3214
BspT107I GGYRCC 3 cut(s) 1907, 2039, 2048
BspTNI GGTCTC 2 cut(s) 2098, 3019
BsrDI GCAATG 4 cut(s) 630, 2436, 2859, 3435
BsrFI RCCGGY 1 cut(s) 1256
BssAI RCCGGY 1 cut(s) 1256
BssECI CCNNGG 6 cut(s) 255, 303, 795, 987, 1641, 2947
BssSI CACGAG 1 cut(s) 2691
BssT1I CCWWGG 5 cut(s) 255, 795, 987, 1641, 2947
Bst2BI CACGAG 1 cut(s) 2691
Bst2UI CCWGG 5 cut(s) 298, 1262, 1947, 2756, 3230
Bst6I CTCTTC 8 cut(s) 33, 86, 672, 1607, 2171, 2253, 2958, 3216
BstAPI GCANNNNNTGC 2 cut(s) 1589, 1598
BstBAI YACGTR 2 cut(s) 1541, 2484
BstBI TTCGAA 1 cut(s) 3214
BstDSI CCRYGG 1 cut(s) 1641
BstENI CCTNNNNNAGG 2 cut(s) 1845, 3365
BstFNI CGCG 1 cut(s) 2341
BstH2I RGCGCY 3 cut(s) 153, 962, 3495
BstHHI GCGC 3 cut(s) 152, 961, 3494
BstMAI GTCTC 4 cut(s) 1799, 2098, 2855, 3019
BstNI CCWGG 5 cut(s) 298, 1262, 1947, 2756, 3230
BstNSI RCATGY 3 cut(s) 1421, 2139, 2578
BstSCI CCNGG 5 cut(s) 296, 1260, 1945, 2754, 3228
BstSFI CTRYAG 3 cut(s) 1893, 2178, 2205
BstSLI GKGCMC 4 cut(s) 879, 1594, 2053, 3361
BstUI CGCG 1 cut(s) 2341
BstV2I GAAGAC 2 cut(s) 3223, 3554
BstX2I RGATCY 5 cut(s) 125, 1086, 1699, 2154, 2881
BstXI CCANNNNNNTGG 1 cut(s) 802
BstYI RGATCY 5 cut(s) 125, 1086, 1699, 2154, 2881
Bsu36I CCTNAGG 1 cut(s) 3366
BsuI GTATCC 2 cut(s) 1011, 2496
BtgI CCRYGG 1 cut(s) 1641
BtgZI GCGATG 1 cut(s) 2322
BtsI GCAGTG 2 cut(s) 820, 2187
CaiI CAGNNNCTG 3 cut(s) 161, 962, 1607
CciI TCATGA 3 cut(s) 2526, 2656, 2814
CfoI GCGC 3 cut(s) 152, 961, 3494
Cfr10I RCCGGY 1 cut(s) 1256
Cfr13I GGNCC 3 cut(s) 135, 300, 1838
CseI GACGC 1 cut(s) 2330
Csp6I GTAC 8 cut(s) 100, 250, 578, 762, 1215, 1890, 2040, 3555
CspCI CAANNNNNGTGG 2 cut(s) 3367, 3402
CviQI GTAC 8 cut(s) 100, 250, 578, 762, 1215, 1890, 2040, 3555
DraI TTTAAA 2 cut(s) 2806, 2889
EaeI YGGCCR 8 cut(s) 792, 1057, 1258, 1939, 3324, 3333, 3415, 3432
Eam1104I CTCTTC 8 cut(s) 33, 86, 672, 1607, 2171, 2253, 2958, 3216
EarI CTCTTC 8 cut(s) 33, 86, 672, 1607, 2171, 2253, 2958, 3216
Eco130I CCWWGG 5 cut(s) 255, 795, 987, 1641, 2947
Eco24I GRGCYC 2 cut(s) 815, 2068
Eco31I GGTCTC 2 cut(s) 2098, 3019
Eco32I GATATC 2 cut(s) 1162, 1411
Eco47I GGWCC 2 cut(s) 300, 1838
Eco47III AGCGCT 1 cut(s) 3493
Eco57I CTGAAG 5 cut(s) 95, 138, 426, 696, 3324
Eco72I CACGTG 1 cut(s) 1541
Eco81I CCTNAGG 1 cut(s) 3366
Eco88I CYCGRG 3 cut(s) 1507, 2087, 3441
EcoNI CCTNNNNNAGG 2 cut(s) 1845, 3365
EcoO109I RGGNCCY 1 cut(s) 135
EcoRII CCWGG 5 cut(s) 296, 1260, 1945, 2754, 3228
EcoRV GATATC 2 cut(s) 1162, 1411
EcoT14I CCWWGG 5 cut(s) 255, 795, 987, 1641, 2947
EcoT38I GRGCYC 2 cut(s) 815, 2068
ErhI CCWWGG 5 cut(s) 255, 795, 987, 1641, 2947
FalI AAGNNNNNCTT 6 cut(s) 239, 271, 453, 485, 1920, 1952
FaqI GGGAC 3 cut(s) 143, 2966, 3062
FbaI TGATCA 1 cut(s) 2493
FblI GTMKAC 1 cut(s) 1993
FriOI GRGCYC 2 cut(s) 815, 2068
GlaI GCGC 3 cut(s) 151, 960, 3493
GsaI CCCAGC 5 cut(s) 666, 812, 1173, 2586, 3162
GsuI CTGGAG 2 cut(s) 2082, 2476
HaeII RGCGCY 3 cut(s) 153, 962, 3495
HapII CCGG 2 cut(s) 1060, 1257
HgaI GACGC 1 cut(s) 2330
HhaI GCGC 3 cut(s) 152, 961, 3494
Hin6I GCGC 3 cut(s) 150, 959, 3492
HinP1I GCGC 3 cut(s) 150, 959, 3492
HindIII AAGCTT 2 cut(s) 228, 408
HpaII CCGG 2 cut(s) 1060, 1257
HphI GGTGA 7 cut(s) 694, 901, 1403, 1499, 1964, 2044, 2870
Hpy166II GTNNAC 7 cut(s) 861, 877, 1036, 1592, 1683, 1994, 3585
Hpy8I GTNNAC 7 cut(s) 861, 877, 1036, 1592, 1683, 1994, 3585
HpyAV CCTTC 8 cut(s) 16, 472, 2452, 2629, 2682, 2975, 3287, 3371
HpyCH4IV ACGT 5 cut(s) 63, 1540, 2483, 2680, 3033
HpySE526I ACGT 5 cut(s) 63, 1540, 2483, 2680, 3033
HspAI GCGC 3 cut(s) 150, 959, 3492
KpnI GGTACC 1 cut(s) 2043
KroI GCCGGC 1 cut(s) 1256
KroNI GCCGGC 1 cut(s) 1258
Ksp22I TGATCA 1 cut(s) 2493
LguI GCTCTTC 1 cut(s) 86
LweI GCATC 7 cut(s) 309, 454, 1384, 1588, 2061, 2344, 2918
MaeII ACGT 5 cut(s) 63, 1540, 2483, 2680, 3033
MfeI CAATTG 2 cut(s) 528, 2988
MflI RGATCY 5 cut(s) 125, 1086, 1699, 2154, 2881
MhlI GDGCHC 7 cut(s) 815, 879, 1594, 2053, 2068, 2555, 3361
MlsI TGGCCA 4 cut(s) 794, 3335, 3417, 3434
MluNI TGGCCA 4 cut(s) 794, 3335, 3417, 3434
MlyI GAGTC 5 cut(s) 648, 911, 1613, 2235, 3515
MmeI TCCRAC 2 cut(s) 296, 3176
Mox20I TGGCCA 4 cut(s) 794, 3335, 3417, 3434
MroNI GCCGGC 1 cut(s) 1256
MroXI GAANNNNTTC 6 cut(s) 80, 1201, 1374, 2522, 2822, 3649
MscI TGGCCA 4 cut(s) 794, 3335, 3417, 3434
MseI TTAA 7 cut(s) 921, 1968, 2148, 2805, 2888, 2996, 3692
MslI CAYNNNNRTG 2 cut(s) 914, 2468
Msp20I TGGCCA 4 cut(s) 794, 3335, 3417, 3434
MspA1I CMGCKG 2 cut(s) 995, 3158
MspI CCGG 2 cut(s) 1060, 1257
MspR9I CCNGG 5 cut(s) 298, 1262, 1947, 2756, 3230
MunI CAATTG 2 cut(s) 528, 2988
Mva1269I GAATGC 2 cut(s) 974, 1699
MvaI CCWGG 5 cut(s) 298, 1262, 1947, 2756, 3230
MvnI CGCG 1 cut(s) 2341
NaeI GCCGGC 1 cut(s) 1258
NcoI CCATGG 1 cut(s) 1641
NgoMIV GCCGGC 1 cut(s) 1256
NheI GCTAGC 1 cut(s) 2578
NlaIV GGNNCC 9 cut(s) 136, 814, 1341, 1745, 1909, 2041, 2050, 2065, 2897
NmuCI GTSAC 4 cut(s) 443, 562, 942, 2504
NspI RCATGY 3 cut(s) 1421, 2139, 2578
NspV TTCGAA 1 cut(s) 3214
OliI CACNNNNGTG 1 cut(s) 2468
PagI TCATGA 3 cut(s) 2526, 2656, 2814
PciSI GCTCTTC 1 cut(s) 86
PctI GAATGC 2 cut(s) 974, 1699
PdiI GCCGGC 1 cut(s) 1258
PdmI GAANNNNTTC 6 cut(s) 80, 1201, 1374, 2522, 2822, 3649
PfeI GAWTC 7 cut(s) 95, 379, 522, 1239, 1555, 1931, 2460
Pfl23II CGTACG 1 cut(s) 99
PflMI CCANNNNNTGG 1 cut(s) 2905
PfoI TCCNGGA 1 cut(s) 296
PleI GAGTC 5 cut(s) 647, 910, 1612, 2235, 3514
PmaCI CACGTG 1 cut(s) 1541
PmlI CACGTG 1 cut(s) 1541
PpsI GAGTC 5 cut(s) 647, 910, 1612, 2235, 3514
Ppu21I YACGTR 2 cut(s) 1541, 2484
Psp1406I AACGTT 1 cut(s) 63
Psp6I CCWGG 5 cut(s) 296, 1260, 1945, 2754, 3228
PspCI CACGTG 1 cut(s) 1541
PspFI CCCAGC 5 cut(s) 662, 808, 1169, 2582, 3158
PspGI CCWGG 5 cut(s) 296, 1260, 1945, 2754, 3228
PspLI CGTACG 1 cut(s) 99
PspN4I GGNNCC 9 cut(s) 136, 814, 1341, 1745, 1909, 2041, 2050, 2065, 2897
PspPI GGNCC 3 cut(s) 135, 300, 1838
PstI CTGCAG 1 cut(s) 2182
PstNI CAGNNNCTG 3 cut(s) 161, 962, 1607
PsuI RGATCY 5 cut(s) 125, 1086, 1699, 2154, 2881
PvuII CAGCTG 2 cut(s) 995, 3158
RsaI GTAC 8 cut(s) 101, 251, 579, 763, 1216, 1891, 2041, 3556
RsaNI GTAC 8 cut(s) 100, 250, 578, 762, 1215, 1890, 2040, 3555
RseI CAYNNNNRTG 2 cut(s) 914, 2468
SapI GCTCTTC 1 cut(s) 86
SaqAI TTAA 7 cut(s) 921, 1968, 2148, 2805, 2888, 2996, 3692
Sau96I GGNCC 3 cut(s) 135, 300, 1838
ScaI AGTACT 1 cut(s) 251
SchI GAGTC 5 cut(s) 648, 911, 1613, 2235, 3515
ScrFI CCNGG 5 cut(s) 298, 1262, 1947, 2756, 3230
SduI GDGCHC 7 cut(s) 815, 879, 1594, 2053, 2068, 2555, 3361
SfaNI GCATC 7 cut(s) 309, 454, 1384, 1588, 2061, 2344, 2918
SfcI CTRYAG 3 cut(s) 1893, 2178, 2205
SfiI GGCCNNNNNGGCC 1 cut(s) 3332
SfuI TTCGAA 1 cut(s) 3214
SinI GGWCC 2 cut(s) 300, 1838
SmiMI CAYNNNNRTG 2 cut(s) 914, 2468
SmlI CTYRAG 2 cut(s) 1076, 2548
SmoI CTYRAG 2 cut(s) 1076, 2548
SsiI CCGC 4 cut(s) 58, 600, 2605, 3323
SspI AATATT 1 cut(s) 1375
StyD4I CCNGG 5 cut(s) 296, 1260, 1945, 2754, 3228
StyI CCWWGG 5 cut(s) 255, 795, 987, 1641, 2947
TaiI ACGT 5 cut(s) 66, 1543, 2486, 2683, 3036
TaqI TCGA 5 cut(s) 399, 2251, 2643, 3023, 3214
TaqII GACCGA 1 cut(s) 317
TatI WGTACW 2 cut(s) 249, 1214
TauI GCSGC 1 cut(s) 3326
TfiI GAWTC 7 cut(s) 95, 379, 522, 1239, 1555, 1931, 2460
Tru1I TTAA 7 cut(s) 921, 1968, 2148, 2805, 2888, 2996, 3692
Tru9I TTAA 7 cut(s) 921, 1968, 2148, 2805, 2888, 2996, 3692
TseFI GTSAC 4 cut(s) 443, 562, 942, 2504
Tsp45I GTSAC 4 cut(s) 443, 562, 942, 2504
TspGWI ACGGA 1 cut(s) 2000
Van91I CCANNNNNTGG 1 cut(s) 2905
VneI GTGCAC 2 cut(s) 875, 1590
VpaK11BI GGWCC 2 cut(s) 300, 1838
XagI CCTNNNNNAGG 2 cut(s) 1845, 3365
XapI RAATTY 2 cut(s) 3465, 3606
XbaI TCTAGA 3 cut(s) 2344, 2675, 3496
XceI RCATGY 3 cut(s) 1421, 2139, 2578
XcmI CCANNNNNNNNNTGG 2 cut(s) 253, 1832
XmaJI CCTAGG 1 cut(s) 2947
XmiI GTMKAC 1 cut(s) 1993
XmnI GAANNNNTTC 6 cut(s) 80, 1201, 1374, 2522, 2822, 3649
ZrmI AGTACT 1 cut(s) 251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.