MD06G1037400.v1.1

ABC transporter B family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0009779
Physical Location & Seq
Reverse (-)
4762831 .. 4770631
7801 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1037400.v1.1.491

Sequence Viewer

Length: 3786 bp
ATGACACAGCCGGACACGTTTACTGGCGGCGGTAGTGGCAACGGCAGCGGTTCCAATTATAGCGATGCGAAGAAGACAAAGAAAGAAGAAGAAGAAGGTAAAGGAAAGCAGCAGCGGAAGGTTTCGTTGTTGAAGCTCTTCTCGTTCGCCGATTCATATGACTACTTGCTAATGGCAGTCGGATCCGTCGGGGCGTGTGTTCATGGAGCTTCAGTTCCTATCTTCTTTATCTTCTTTGGCAAGTTGATCAATATTATTGGCATGGCTTATCTTTTTCCCAAAGAAGCTTCTTCCAAAGTTGCTAAGTACTCGTTGGATTTTGTATATCTGAGTGTGGCTATATTGTTTTCTTCATGGACGGAGGTGGCTTGCTGGATGCACACTGGGGAAAGACAAGCGGCAAAAATGAGGATGGCATATTTGAGGGCGATGCTGAATCAAGACATAAGTCTCTTTGACACTGAAGCTTCCACAGGAGAGGTCATCTCTGCCATCACAAGTGACATAATTGTTGTTCAAGATGCACTTTCTGAGAAGGTAGGGAACTTCTTGCATTACGTAAGCCGGTTTCTAGCAGGTTTCATAATTGGGTTTGTTAGGGTATGGCAAATCAGTCTGGTCACACTCTCCATTGTTCCATTAATTGCCCTTGCTGGTGGCGTCTATGCCTACGTCACAATTGGCCTCATTGCTAGGGTTCGCAAATCCTACGTCAAAGCCGGTGAAATTGCCGAGGAGGTGATCGGAAATGTGAGGACGGTGCAAGCATTTGCCGCAGAAGACAAGGCGGTAAGAGAGTATAAGGCAGCACTCTTGAAAACGTACAAGTATGGGAAGAAAGCAGGACTGGCCAAGGGTCTTGGCCTTGGCTCCATGCACTGCTCCCTTTTCCTTTCGTGGTCTTTGCTTGTTTGGTTCACTAGCATTGTTGTTCACAAGGGTATTGCCAATGGCGGCGAATCCTTCACCACCATGCTTAACGTTGTTATTGCTGGCCTGTCACTAGGACAGGCCGCACCAGACATCACTGCATTCATTCGAGCCAAGGCAGCCGCGTATCCCATATTTGAAATGATAGAGAGGAACACGATGAGCCAATCTAGCAGCAATAATGGCCACAAACTGAACAAAATTGAGGGTCATATACAATTTAAGGACGTATGTTTTAGTTACCCGTCTCGTTCTGATGTTAGCATCTTCAACAAGCTCAATCTTGATATCCCTGCCGGAAAGATTGTAGCTCTTGTGGGAGGCAGTGGATCGGGAAAGAGTACAGCTATATCCTTGATCGAACGCTTCTACGAGCCACTTGCTGGCCAGATATTATTAGATGGACATAATATCAAGGAGCTTGACATCAAATGGCTTAGGCAGCAAATTGGGCTGGTCAACCAGGAGCCTGCCCTTTTTGCAACAAGCATTAAGGAAAATATTCTCTATGGGAAATATGATGCTACATTCGACGAGATCACACGTGCAGCTAAACTTTCAGAAGCTCTCTCTTTCGTAAATAATCTCCCAGAGAGATTTGAAACTCAGGTTGGTGAGAGAGGAATACAACTTTCAGGAGGACAAAAGCAAAGGATTGCAATAGCACGTGCAATAGTGAAGAATCCGTCAGTTCTCCTATTGGATGAAGCAACAAGCGCACTGGACGCAGAGTCTGAGAAGAGTGTGCAGGAGGCACTTGATCGTGCCATGGTGGGACGAACAACAGTAGTGGTGGCCCATCGTCTTTCTACGGTTAGGAATGCAGATGTAATTGCTGTTGTTCAAGAGGGTAAGATAGTGGAAACCGGGAGCCATGAGGAGCTCATTTCTAATCCAAACGGTGTCTATGCAGCACTAGTTCAATTACAAGAGACAGCTTCTTTGCAACGCCACCCCTCAATTGGTAACTTGGGAAGGCCACTAAGTGTGAGGAATTCACGAGAATTATCCCGCACTACAACAAGTTTCGGAGCCAGCTTCCGTTCTGATAAAGAATCTGTTGGCCGTACTGGTGCTGATGTAATGGAGACTGTAAAGTCAAAACATGTTTCAGCAGGGAGACTGTATTCCATGGTTGGTCCAGACTGGTATTATGGCGTGATAGGCACCTTTGGTGGCCTTATTGCTGGAGCTCAGATGCCTCTTTTTGCTCTCGGGGTCTCTCAGGCTCTTGTTTCATTTTACATGGACTGGGAGACAACAAAACATGAGGTTAAAAAAATCTCTTTACTCTTCTGCGGGGCTGCATTTTTAACTGTCATTGTTCATGCCATAGAGCATCTTTGTTTTGGGATTATGGGAGAGCGACTCACTCTTCGTGTTAGAGAAAAGATGTTTTCCGCCATATTGAGGAATGAGATCGGATGGTTTGATGATACAAACAACACTAGTTCTATGCTTTCATCACGTCTAGAAAGTGATGCAACTTTGTTAAGAACTATTGTTGTTGATCGGACAACAATTCTTATACAGAATTTGGGTTTGGTTGTTGCCTCATTCGTCATCGCCTTCATCTTGAACTGGAGAATTACACTCGTGGTCATAGCCACATATCCCTTGATCATTAGTGGTCACATAAGCGAGAAACTTTTCTTTCAAGGCTACGGTGGAAACTTGAGCAAAGCATATCTAAAAGCTAACATGCTAGCCGGGGAGGCTGTGAGTAACATCCGCACTGTTGCTGCATTTTGTTCTGAGGAGAAGATCATCGACCTTTATGCTCGTGAGCTTGTGGATCCTTCTAGACGTTCATTTACTCGCGGTCAGATTGCTGGCATATTCTACGGTGTATCCCAATTCTTCATATTCTCGTCCTATGGCCTGGCCTTGTGGTATGGTTCTGTTTTAATGGGAAAGGAGCTCGCAAGCTTTAAATCTGTCATGAAATCTTTCATGGTTTTGATTGTGACGGCATTAGCTATGGGTGAGACTTTAGCACTTGCCCCAGACCTCTTGAAAGGGAACCAAATGGTGTCATCAGTCTTTGAAGTATTGGATCGTGGAACCGAAGTCCTGGGAGACGTAGGAGAGGAGCTTATGAAAGTTGAAGGTACAATAGAGCTAAGGAGTGTTCACTTCGGCTACCCTTCAAGACCAGACGTATCCATTTTCAAGGACTTCAATTTGAAAGTGCGCTCGGGCAAAAGTATGGCACTAGTGGGGCAAAGTGGATCTGGAAAAAGCTCTGTGCTTTCCCTAATACTCCGATTTTACGATCCAACCTCTGGGAAAGTCATGATTGATGGGAAAGACATCAAGAAACTCAATATCAAGTCTCTTCGTAAACACATTGGCTTGGTTCAACAAGAACCAGCTCTCTTTGCCACATCAATTTATGAAAACATCCTCTATGGAAAAGATGGAGCTTCTGAAGCAGAAGTTATTGAAGCGGCTAAGCTTGCTAATGCGCACAGTTTCATCAGTGCCCTTCCTGAGGGATACTTAACCAAAGTCGGAGAGCGAGGGGTGCAACTATCTGGTGGTCAAAGGCAAAGGGTAGCCATTGCCCGTGCCGTTCTAAAGAACCCAGAAATCTTGTTATTAGATGAAGCTACCAGTGCTCTAGATGTGGAGTCGGAGCGCGTGGTGCAACAAGCTCTTGACAGATTGATGAAGAACCGTACAACGGTATTGGTGGCACATAGGCTTTCGACAATAAAAAATGCAGATGAAATCTCAGTTATACAAGACGGGAAGATTGTTGAGCAAGGAGGTCACTCGACACTTATAGAGAACAGAAATGGACCATATTTCAAGTTAATAAACATTCAGCAACAGCAGCAACAACAACAGTAA

Protein Analysis

1262

Amino Acids

137.81

Weight (kDa)

8.93

Isoelectric Point (pI)

34.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane PF00664 63 - 330 3.6e-47 ABC transporter transmembrane region
ABC_tran PF00005 401 - 549 2e-34 ABC transporter
ABC_membrane PF00664 698 - 968 1.7e-46 ABC transporter transmembrane region
ABC_tran PF00005 1035 - 1183 9.6e-35 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0009779)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10680 AT1G10680 AT4G25960
fragaria_vesca FvH4_5g22520 FvH4_5g22520 FvH4_5g22520 FvH4_5g22520 FvH4_5g22520 FvH4_5g22520
malus_domestica MD06G1037400.v1.1
prunus_persica Prupe.5G031600_v2.0.a1
pyrus_communis pycom06g03090
rosa_chinensis RchiOBHm_Chr7g0210291
rosa_laevigata RLG00000003051 RLG00000029346
rosa_multiflora Rmu_sc0003639.1_g000002
rosa_roxburghii Rroxscaffold_3G00248450
rosa_rugosa Rorug07G0120500
rosa_samantha Rh7AG253400 Rh7CG270300
rosa_wichuraiana Rw7G021600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2026
Acc16I TGCGCA 1 cut(s) 3399
Acc36I ACCTGC 1 cut(s) 566
AccB1I GGYRCC 1 cut(s) 2096
AccB7I CCANNNNNTGG 2 cut(s) 1313, 3215
AccII CGCG 3 cut(s) 1055, 2751, 3573
AclI AACGTT 1 cut(s) 981
AclWI GGATC 8 cut(s) 177, 190, 1267, 2720, 2733, 2994, 3169, 3200
AcoI YGGCCR 4 cut(s) 849, 1114, 1315, 1993
AcsI RAATTY 2 cut(s) 1924, 2464
AcuI CTGAAG 3 cut(s) 195, 483, 3381
AcvI CACGTG 2 cut(s) 1475, 1598
AcyI GRCGYC 1 cut(s) 660
AdeI CACNNNGTG 1 cut(s) 1916
AfaI GTAC 6 cut(s) 308, 824, 1273, 1999, 3043, 3613
AfiI CCNNNNNNNGG 6 cut(s) 1313, 1892, 2340, 3215, 3424, 3616
AflIII ACRYGT 3 cut(s) 15, 1472, 2035
AhdI GACNNNNNGTC 1 cut(s) 1660
AhlI ACTAGT 3 cut(s) 1846, 2378, 3145
AjiI CACGTC 1 cut(s) 2399
AjnI CCWGG 3 cut(s) 1392, 2811, 3003
AjuI GAANNNNNNNTTGG 4 cut(s) 1524, 1556, 2456, 2488
AloI GAACNNNNNNTCC 2 cut(s) 198, 230
Alw21I GWGCWC 4 cut(s) 1815, 2125, 2853, 3553
AlwI GGATC 8 cut(s) 177, 190, 1267, 2720, 2733, 2994, 3169, 3200
AlwNI CAGNNNCTG 1 cut(s) 1664
Ama87I CYCGRG 2 cut(s) 2144, 3127
ApoI RAATTY 2 cut(s) 1924, 2464
ArsI GACNNNNNNTTYG 6 cut(s) 696, 728, 1855, 1887, 2289, 2321
AseI ATTAAT 1 cut(s) 641
Asp700I GAANNNNTTC 4 cut(s) 137, 1431, 2579, 2879
AspLEI GCGC 4 cut(s) 1649, 3126, 3400, 3573
AspS9I GGNCC 3 cut(s) 1726, 2069, 3734
AsuC2I CCSGG 2 cut(s) 1798, 2641
AsuHPI GGTGA 5 cut(s) 734, 751, 958, 1556, 2927
AsuNHI GCTAGC 1 cut(s) 2635
AvaI CYCGRG 2 cut(s) 2144, 3127
AvaII GGWCC 2 cut(s) 2069, 3734
AxyI CCTNAGG 1 cut(s) 3423
BaeGI GKGCMC 1 cut(s) 3418
BalI TGGCCA 3 cut(s) 851, 1116, 1317
BamHI GGATCC 2 cut(s) 182, 2725
BanI GGYRCC 1 cut(s) 2096
BanII GRGCYC 3 cut(s) 1815, 2125, 2853
BauI CACGAG 3 cut(s) 1929, 2525, 2712
BbrPI CACGTG 2 cut(s) 1475, 1598
BbsI GAAGAC 2 cut(s) 80, 786
Bbv12I GWGCWC 4 cut(s) 1815, 2125, 2853, 3553
BccI CCATC 7 cut(s) 406, 500, 1325, 1737, 2349, 3227, 3344
BceAI ACGGC 4 cut(s) 58, 1980, 2916, 3488
BcgI CGANNNNNNTGC 4 cut(s) 1292, 1326, 2690, 2724
BciT130I CCWGG 3 cut(s) 1394, 2813, 3005
BciVI GTATCC 4 cut(s) 1068, 2791, 3103, 3422
BclI TGATCA 2 cut(s) 246, 2550
BcnI CCSGG 2 cut(s) 1798, 2641
BcuI ACTAGT 3 cut(s) 1846, 2378, 3145
BfuAI ACCTGC 1 cut(s) 566
BfuI GTATCC 4 cut(s) 1068, 2791, 3103, 3422
BglI GCCNNNNNGGC 1 cut(s) 2645
BlpI GCTNAGC 1 cut(s) 3384
BmcAI AGTACT 1 cut(s) 308
Bme1390I CCNGG 5 cut(s) 1394, 1798, 2641, 2813, 3005
Bme18I GGWCC 2 cut(s) 2069, 3734
BmeRI GACNNNNNGTC 1 cut(s) 1660
BmeT110I CYCGRG 2 cut(s) 2144, 3127
BmgBI CACGTC 1 cut(s) 2399
BmgT120I GGNCC 3 cut(s) 1726, 2069, 3734
BmrFI CCNGG 5 cut(s) 1394, 1798, 2641, 2813, 3005
BmrI ACTGGG 2 cut(s) 393, 2191
BmsI GCATC 9 cut(s) 55, 366, 420, 511, 1203, 1441, 2118, 2278, 2401
BmtI GCTAGC 1 cut(s) 2639
BmuI ACTGGG 2 cut(s) 393, 2191
BoxI GACNNNNGTC 1 cut(s) 447
BpiI GAAGAC 2 cut(s) 80, 786
BplI GAGNNNNNCTC 4 cut(s) 470, 502, 2283, 2315
BpmI CTGGAG 2 cut(s) 2139, 2533
Bpu10I CCTNAGC 2 cut(s) 1367, 3053
Bpu1102I GCTNAGC 1 cut(s) 3384
BpuEI CTTGAG 1 cut(s) 2626
BpuMI CCSGG 2 cut(s) 1798, 2641
BsaAI YACGTR 3 cut(s) 559, 1475, 1598
BsaHI GRCGYC 1 cut(s) 660
BsaI GGTCTC 1 cut(s) 2155
BsaJI CCNNGG 8 cut(s) 732, 852, 865, 1044, 1698, 2061, 2640, 3004
BsaXI ACNNNNNCTCC 2 cut(s) 198, 228
Bsc4I CCNNNNNNNGG 6 cut(s) 1313, 1892, 2340, 3215, 3424, 3616
Bse118I RCCGGY 2 cut(s) 564, 719
Bse1I ACTGG 9 cut(s) 28, 388, 852, 1656, 2005, 2081, 2186, 2516, 3546
Bse21I CCTNAGG 1 cut(s) 3423
Bse3DI GCAATG 2 cut(s) 687, 3492
BseBI CCWGG 3 cut(s) 1394, 2813, 3005
BseDI CCNNGG 8 cut(s) 732, 852, 865, 1044, 1698, 2061, 2640, 3004
BseGI GGATG 6 cut(s) 381, 417, 1639, 2360, 2658, 3333
BseLI CCNNNNNNNGG 6 cut(s) 1313, 1892, 2340, 3215, 3424, 3616
BseMI GCAATG 2 cut(s) 687, 3492
BseMII CTCAG 9 cut(s) 320, 522, 1550, 1656, 2138, 2168, 2676, 3414, 3681
BseNI ACTGG 9 cut(s) 28, 388, 852, 1656, 2005, 2081, 2186, 2516, 3546
BseRI GAGGAG 4 cut(s) 749, 1823, 2702, 3035
BseSI GKGCMC 1 cut(s) 3418
BsgI GTGCAG 2 cut(s) 1497, 1697
Bsh1236I CGCG 3 cut(s) 1055, 2751, 3573
BshNI GGYRCC 1 cut(s) 2096
BsiHKAI GWGCWC 4 cut(s) 1815, 2125, 2853, 3553
BsiHKCI CYCGRG 2 cut(s) 2144, 3127
BsiSI CCGG 6 cut(s) 11, 565, 720, 1227, 1797, 2640
BslFI GGGAC 1 cut(s) 1719
BslI CCNNNNNNNGG 6 cut(s) 1313, 1892, 2340, 3215, 3424, 3616
BsmBI CGTCTC 2 cut(s) 1182, 3003
BsmFI GGGAC 1 cut(s) 1719
BsmI GAATGC 2 cut(s) 1031, 1756
Bso31I GGTCTC 1 cut(s) 2155
BsoBI CYCGRG 2 cut(s) 2144, 3127
Bsp1286I GDGCHC 5 cut(s) 1815, 2125, 2853, 3418, 3553
Bsp1720I GCTNAGC 1 cut(s) 3384
Bsp19I CCATGG 2 cut(s) 1698, 2061
BspCNI CTCAG 9 cut(s) 321, 523, 1549, 1657, 2137, 2167, 2677, 3415, 3680
BspFNI CGCG 3 cut(s) 1055, 2751, 3573
BspHI TCATGA 2 cut(s) 2871, 3225
BspMI ACCTGC 1 cut(s) 566
BspOI GCTAGC 1 cut(s) 2639
BspPI GGATC 8 cut(s) 177, 190, 1267, 2720, 2733, 2994, 3169, 3200
BspQI GCTCTTC 1 cut(s) 143
BspT107I GGYRCC 1 cut(s) 2096
BspTNI GGTCTC 1 cut(s) 2155
BsrDI GCAATG 2 cut(s) 687, 3492
BsrFI RCCGGY 2 cut(s) 564, 719
BsrI ACTGG 9 cut(s) 28, 388, 852, 1656, 2005, 2081, 2186, 2516, 3546
BssAI RCCGGY 2 cut(s) 564, 719
BssECI CCNNGG 8 cut(s) 732, 852, 865, 1044, 1698, 2061, 2640, 3004
BssNI GRCGYC 1 cut(s) 660
BssSI CACGAG 3 cut(s) 1929, 2525, 2712
BssT1I CCWWGG 5 cut(s) 852, 865, 1044, 1698, 2061
Bst2BI CACGAG 3 cut(s) 1929, 2525, 2712
Bst2UI CCWGG 3 cut(s) 1394, 2813, 3005
Bst6I CTCTTC 5 cut(s) 143, 1664, 2228, 2310, 3273
BstACI GRCGYC 1 cut(s) 660
BstBAI YACGTR 3 cut(s) 559, 1475, 1598
BstDSI CCRYGG 2 cut(s) 1698, 2061
BstENI CCTNNNNNAGG 1 cut(s) 3422
BstF5I GGATG 6 cut(s) 381, 417, 1639, 2360, 2658, 3333
BstFNI CGCG 3 cut(s) 1055, 2751, 3573
BstHHI GCGC 4 cut(s) 1649, 3126, 3400, 3573
BstNI CCWGG 3 cut(s) 1394, 2813, 3005
BstNSI RCATGY 2 cut(s) 2039, 2635
BstPAI GACNNNNGTC 1 cut(s) 447
BstSCI CCNGG 5 cut(s) 1392, 1796, 2639, 2811, 3003
BstSLI GKGCMC 1 cut(s) 3418
BstSNI TACGTA 1 cut(s) 559
BstUI CGCG 3 cut(s) 1055, 2751, 3573
BstV2I GAAGAC 2 cut(s) 80, 786
BstX2I RGATCY 3 cut(s) 182, 2725, 3161
BstYI RGATCY 3 cut(s) 182, 2725, 3161
Bsu36I CCTNAGG 1 cut(s) 3423
BsuI GTATCC 4 cut(s) 1068, 2791, 3103, 3422
BtgI CCRYGG 2 cut(s) 1698, 2061
BtgZI GCGATG 3 cut(s) 78, 443, 2479
BtrI CACGTC 1 cut(s) 2399
BtsCI GGATG 6 cut(s) 381, 417, 1639, 2360, 2658, 3333
BtsI GCAGTG 3 cut(s) 877, 1026, 1261
BtsIMutI CAGTG 9 cut(s) 381, 459, 877, 1026, 1261, 1649, 2664, 3418, 3553
BveI ACCTGC 1 cut(s) 566
CaiI CAGNNNCTG 1 cut(s) 1664
CciI TCATGA 2 cut(s) 2871, 3225
CfoI GCGC 4 cut(s) 1649, 3126, 3400, 3573
Cfr10I RCCGGY 2 cut(s) 564, 719
Cfr13I GGNCC 3 cut(s) 1726, 2069, 3734
CseI GACGC 2 cut(s) 649, 1664
Csp6I GTAC 6 cut(s) 307, 823, 1272, 1998, 3042, 3612
CspCI CAANNNNNGTGG 2 cut(s) 1701, 1736
CviQI GTAC 6 cut(s) 307, 823, 1272, 1998, 3042, 3612
DraI TTTAAA 1 cut(s) 2863
DraIII CACNNNGTG 1 cut(s) 1916
DrdI GACNNNNNNGTC 1 cut(s) 2026
DriI GACNNNNNGTC 1 cut(s) 1660
DseDI GACNNNNNNGTC 1 cut(s) 2026
EaeI YGGCCR 4 cut(s) 849, 1114, 1315, 1993
Eam1104I CTCTTC 5 cut(s) 143, 1664, 2228, 2310, 3273
Eam1105I GACNNNNNGTC 1 cut(s) 1660
EarI CTCTTC 5 cut(s) 143, 1664, 2228, 2310, 3273
EciI GGCGGA 1 cut(s) 2320
Ecl136II GAGCTC 3 cut(s) 1813, 2123, 2851
Eco105I TACGTA 1 cut(s) 559
Eco130I CCWWGG 5 cut(s) 852, 865, 1044, 1698, 2061
Eco24I GRGCYC 3 cut(s) 1815, 2125, 2853
Eco31I GGTCTC 1 cut(s) 2155
Eco32I GATATC 1 cut(s) 1219
Eco47I GGWCC 2 cut(s) 2069, 3734
Eco53kI GAGCTC 3 cut(s) 1813, 2123, 2851
Eco57I CTGAAG 3 cut(s) 195, 483, 3381
Eco72I CACGTG 2 cut(s) 1475, 1598
Eco81I CCTNAGG 1 cut(s) 3423
Eco88I CYCGRG 2 cut(s) 2144, 3127
EcoICRI GAGCTC 3 cut(s) 1813, 2123, 2851
EcoNI CCTNNNNNAGG 1 cut(s) 3422
EcoRI GAATTC 1 cut(s) 1924
EcoRII CCWGG 3 cut(s) 1392, 2811, 3003
EcoRV GATATC 1 cut(s) 1219
EcoT14I CCWWGG 5 cut(s) 852, 865, 1044, 1698, 2061
EcoT38I GRGCYC 3 cut(s) 1815, 2125, 2853
ErhI CCWWGG 5 cut(s) 852, 865, 1044, 1698, 2061
Esp3I CGTCTC 2 cut(s) 1182, 3003
FalI AAGNNNNNCTT 2 cut(s) 510, 542
FaqI GGGAC 1 cut(s) 1719
FauI CCCGC 2 cut(s) 1949, 2222
FauNDI CATATG 1 cut(s) 157
FbaI TGATCA 2 cut(s) 246, 2550
FokI GGATG 6 cut(s) 388, 424, 1646, 2367, 2645, 3320
FriOI GRGCYC 3 cut(s) 1815, 2125, 2853
FspAI RTGCGCAY 1 cut(s) 3399
FspI TGCGCA 1 cut(s) 3399
GlaI GCGC 4 cut(s) 1648, 3125, 3399, 3572
GsuI CTGGAG 2 cut(s) 2139, 2533
HapII CCGG 6 cut(s) 11, 565, 720, 1227, 1797, 2640
HgaI GACGC 2 cut(s) 649, 1664
HhaI GCGC 4 cut(s) 1649, 3126, 3400, 3573
Hin1I GRCGYC 1 cut(s) 660
Hin6I GCGC 4 cut(s) 1647, 3124, 3398, 3571
HinP1I GCGC 4 cut(s) 1647, 3124, 3398, 3571
HincII GTYRAC 1 cut(s) 1390
HindII GTYRAC 1 cut(s) 1390
HindIII AAGCTT 4 cut(s) 285, 465, 2857, 3386
HinfI GANTC 8 cut(s) 152, 436, 959, 1612, 1661, 1985, 2298, 3563
HpaII CCGG 6 cut(s) 11, 565, 720, 1227, 1797, 2640
HphI GGTGA 5 cut(s) 734, 751, 958, 1556, 2927
Hpy166II GTNNAC 6 cut(s) 21, 918, 934, 1390, 3064, 3275
Hpy8I GTNNAC 6 cut(s) 21, 918, 934, 1390, 3064, 3275
Hpy99I CGWCG 2 cut(s) 191, 1466
Hsp92I GRCGYC 1 cut(s) 660
HspAI GCGC 4 cut(s) 1647, 3124, 3398, 3571
Ksp22I TGATCA 2 cut(s) 246, 2550
LguI GCTCTTC 1 cut(s) 143
LweI GCATC 9 cut(s) 55, 366, 420, 511, 1203, 1441, 2118, 2278, 2401
MfeI CAATTG 2 cut(s) 678, 1890
MflI RGATCY 3 cut(s) 182, 2725, 3161
MhlI GDGCHC 5 cut(s) 1815, 2125, 2853, 3418, 3553
MlsI TGGCCA 3 cut(s) 851, 1116, 1317
MluNI TGGCCA 3 cut(s) 851, 1116, 1317
MlyI GAGTC 3 cut(s) 1670, 2292, 3572
MmeI TCCRAC 5 cut(s) 160, 294, 3233, 3424, 3546
Mox20I TGGCCA 3 cut(s) 851, 1116, 1317
MroXI GAANNNNTTC 4 cut(s) 137, 1431, 2579, 2879
MscI TGGCCA 3 cut(s) 851, 1116, 1317
MslI CAYNNNNRTG 1 cut(s) 971
Msp20I TGGCCA 3 cut(s) 851, 1116, 1317
MspA1I CMGCKG 2 cut(s) 48, 115
MspI CCGG 6 cut(s) 11, 565, 720, 1227, 1797, 2640
MspR9I CCNGG 5 cut(s) 1394, 1798, 2641, 2813, 3005
MunI CAATTG 2 cut(s) 678, 1890
Mva1269I GAATGC 2 cut(s) 1031, 1756
MvaI CCWGG 3 cut(s) 1394, 2813, 3005
MvnI CGCG 3 cut(s) 1055, 2751, 3573
NciI CCSGG 2 cut(s) 1798, 2641
NcoI CCATGG 2 cut(s) 1698, 2061
NdeI CATATG 1 cut(s) 157
NheI GCTAGC 1 cut(s) 2635
NmeAIII GCCGAG 1 cut(s) 757
NmuCI GTSAC 7 cut(s) 500, 619, 673, 999, 2561, 2896, 3704
NsbI TGCGCA 1 cut(s) 3399
NspI RCATGY 2 cut(s) 2039, 2635
PagI TCATGA 2 cut(s) 2871, 3225
PciI ACATGT 1 cut(s) 2035
PciSI GCTCTTC 1 cut(s) 143
PctI GAATGC 2 cut(s) 1031, 1756
PdmI GAANNNNTTC 4 cut(s) 137, 1431, 2579, 2879
PfeI GAWTC 5 cut(s) 152, 436, 959, 1612, 1985
PflMI CCANNNNNTGG 2 cut(s) 1313, 3215
PleI GAGTC 3 cut(s) 1669, 2292, 3571
PmaCI CACGTG 2 cut(s) 1475, 1598
PmlI CACGTG 2 cut(s) 1475, 1598
PpsI GAGTC 3 cut(s) 1669, 2292, 3571
Ppu21I YACGTR 3 cut(s) 559, 1475, 1598
PscI ACATGT 1 cut(s) 2035
PshAI GACNNNNGTC 1 cut(s) 447
PshBI ATTAAT 1 cut(s) 641
Psp124BI GAGCTC 3 cut(s) 1815, 2125, 2853
Psp1406I AACGTT 1 cut(s) 981
Psp6I CCWGG 3 cut(s) 1392, 2811, 3003
PspCI CACGTG 2 cut(s) 1475, 1598
PspGI CCWGG 3 cut(s) 1392, 2811, 3003
PspPI GGNCC 3 cut(s) 1726, 2069, 3734
PstNI CAGNNNCTG 1 cut(s) 1664
PsuI RGATCY 3 cut(s) 182, 2725, 3161
RsaI GTAC 6 cut(s) 308, 824, 1273, 1999, 3043, 3613
RsaNI GTAC 6 cut(s) 307, 823, 1272, 1998, 3042, 3612
RseI CAYNNNNRTG 1 cut(s) 971
SacI GAGCTC 3 cut(s) 1815, 2125, 2853
SapI GCTCTTC 1 cut(s) 143
Sau96I GGNCC 3 cut(s) 1726, 2069, 3734
ScaI AGTACT 1 cut(s) 308
SchI GAGTC 3 cut(s) 1670, 2292, 3572
ScrFI CCNGG 5 cut(s) 1394, 1798, 2641, 2813, 3005
SduI GDGCHC 5 cut(s) 1815, 2125, 2853, 3418, 3553
SfaNI GCATC 9 cut(s) 55, 366, 420, 511, 1203, 1441, 2118, 2278, 2401
SinI GGWCC 2 cut(s) 2069, 3734
SmiMI CAYNNNNRTG 1 cut(s) 971
SmlI CTYRAG 1 cut(s) 2605
SmoI CTYRAG 1 cut(s) 2605
SnaBI TACGTA 1 cut(s) 559
SpeI ACTAGT 3 cut(s) 1846, 2378, 3145
SspI AATATT 2 cut(s) 253, 1432
SstI GAGCTC 3 cut(s) 1815, 2125, 2853
StyD4I CCNGG 5 cut(s) 1392, 1796, 2639, 2811, 3003
StyI CCWWGG 5 cut(s) 852, 865, 1044, 1698, 2061
TaqI TCGA 6 cut(s) 1039, 1290, 1461, 2700, 3641, 3710
TatI WGTACW 2 cut(s) 306, 1271
TauI GCSGC 7 cut(s) 30, 401, 776, 957, 1016, 1055, 3383
TfiI GAWTC 5 cut(s) 152, 436, 959, 1612, 1985
TscAI CASTG 9 cut(s) 388, 466, 884, 1033, 1261, 1656, 2671, 3418, 3553
TseFI GTSAC 7 cut(s) 500, 619, 673, 999, 2561, 2896, 3704
Tsp45I GTSAC 7 cut(s) 500, 619, 673, 999, 2561, 2896, 3704
TspGWI ACGGA 4 cut(s) 175, 374, 1605, 1961
TspRI CASTG 9 cut(s) 388, 466, 884, 1033, 1261, 1656, 2671, 3418, 3553
Van91I CCANNNNNTGG 2 cut(s) 1313, 3215
VpaK11BI GGWCC 2 cut(s) 2069, 3734
VspI ATTAAT 1 cut(s) 641
XagI CCTNNNNNAGG 1 cut(s) 3422
XapI RAATTY 2 cut(s) 1924, 2464
XbaI TCTAGA 3 cut(s) 2401, 2732, 3553
XceI RCATGY 2 cut(s) 2039, 2635
XcmI CCANNNNNNNNNTGG 1 cut(s) 1889
XmnI GAANNNNTTC 4 cut(s) 137, 1431, 2579, 2879
ZrmI AGTACT 1 cut(s) 308
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.