AT4G25960

coupled to transmembrane movement of substances

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0009779
Physical Location & Seq
Forward (+)
13177356 .. 13183692
6337 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G25960.1

Sequence Viewer

Length: 3822 bp
ATGTATATCTCTCTCATCTTCTTCCTCTCTAACCACTTTCCTCCCTTAATTTCTATCCCTATATTTATTTTCCTTTCATTTTCCTCTCCAACTAATTACACACACTTGAAGCTGAAAAAAATGCAGCCGTCCGGTGATCCGGCGCCGGAAAAGGAGAAAGAGATGACGCAGCCAAAAGTGTCACTGCTTAAGCTCTTCTCCTTCGCAGATTTCTATGATTGTGTTCTAATGACTCTTGGATCCGTAGGAGCGTGCATTCATGGCGCCTCGGTTCCAATCTTTTTCATCTTCTTTGGCAAACTCATCAATATTATCGGTCTTGCTTATCTTTTCCCAAAACAAGCCTCTCACAGAGTCGCCAAGTACTCGTTGGATTTTGTATACTTGAGTGTGGCTATACTATTTTCATCATGGTTAGAGGTTGCATGTTGGATGCATACGGGAGAGAGGCAAGCGGCGAAGATGAGGAGAGCTTATCTCAGGTCAATGTTAAGCCAAGACATAAGCTTATTCGACACCGAAGCTTCTACAGGAGAAGTCATCTCCGCCATCACCTCCGACATCCTCGTCGTCCAAGATGCTCTCTCCGAGAAGGTAGGGAATTTCTTGCACTACATAAGCCGGTTCATAGCCGGTTTTGCAATTGGATTCACTAGTGTATGGCAAATAAGTCTTGTCACTCTATCCATAGTCCCCTTAATCGCTCTAGCTGGCGGCATCTATGCGTTCGTCGCCATCGGACTTATTGCTCGTGTCCGGAAATCATACATCAAGGCTGGTGAGATTGCAGAAGAGGTGATCGGGAATGTGAGGACCGTACAAGCATTCACCGGTGAAGAAAGGGCGGTGAGATTATACCGAGAAGCGCTGGAGAATACGTATAAGTACGGGAGAAAAGCTGGTTTAACCAAAGGATTAGGTCTTGGATCGATGCACTGTGTCTTGTTTCTATCTTGGGCCTTGCTCGTATGGTTCACAAGTGTTGTGGTCCACAAGGATATCGCCGATGGTGGCAAATCATTCACTACCATGCTCAACGTTGTCATCGCCGGCCTGTCTCTTGGACAAGCGGCGCCCGATATTTCTGCGTTTGTACGGGCAAAAGCCGCTGCTTATCCAATTTTCAAGATGATTGAACGGAACACGGTGACAAAAACAAGCGCAAAATCTGGCCGTAAACTAGGGAAAGTAGACGGGCACATTCAATTCAAAGACGCGACTTTCAGTTACCCATCTCGCCCTGATGTGGTGATCTTCGACAGGTTAAACCTAGCTATCCCTGCCGGGAAAATTGTGGCACTTGTTGGAGGAAGCGGATCAGGGAAGAGCACGGTCATATCTTTGATAGAGCGGTTCTACGAGCCTATTTCTGGAGCAGTGTTGCTAGACGGGAACAATATCAGTGAGCTGGATATTAAGTGGTTAAGAGGACAGATTGGTTTGGTTAACCAAGAACCAGCTCTGTTTGCAACAACGATTCGTGAGAACATTCTGTATGGCAAAGACGACGCAACTGCTGAGGAGATCACCCGTGCCGCAAAGCTCTCGGAAGCCATTTCTTTCATCAACAACCTCCCTGAAGGATTTGAGACTCAGGTTGGCGAGAGAGGGATTCAACTATCTGGCGGACAGAAACAAAGGATTGCTATATCAAGAGCAATTGTGAAGAATCCATCGATACTCTTACTCGACGAAGCAACAAGTGCTTTAGACGCAGAGTCGGAGAAGAGCGTGCAAGAAGCGCTGGATCGAGTGATGGTTGGGAGAACAACGGTGGTGGTGGCTCACAGACTCTCAACGGTAAGAAATGCTGACATCATAGCAGTTGTACACGAAGGTAAAATAGTTGAGTTTGGAAACCACGAGAATCTCATATCTAATCCCGACGGAGCCTACTCTTCTCTTCTTCGTCTCCAAGAGACTGCCTCATTGCAGCGTAACCCTTCCTTGAATCGCACGCTAAGCAGACCACATAGTATAAAATACTCGCGGGAACTATCAAGAACAAGATCCAGCTTCTGTTCAGAGAGGGAATCGGTAACTCGACCGGATGGGGCTGATCCATCCAAAAAAGTGAAAGTAACGGTGGGACGTCTTTACTCTATGATCCGTCCGGACTGGATGTATGGCGTGTGTGGCACGATATGTGCCTTTATCGCTGGATCTCAAATGCCCCTTTTCGCGCTTGGCGTCTCGCAAGCCTTAGTTTCCTATTACAGTGGTTGGGATGAGACTCAAAAAGAGATCAAGAAGATCGCTATCCTCTTCTGCTGCGCGTCCGTTATCACTCTAATTGTCTACACCATTGAGCATATCTGCTTTGGCACTATGGGCGAGCGTCTCACTCTCCGTGTCCGCGAAAATATGTTCAGAGCAATCTTGAAGAATGAAATCGGGTGGTTTGATGAGGTGGACAACACAAGCTCAATGTTGGCATCGCGGCTTGAGAGCGATGCAACTTTGCTTAAAACCATAGTGGTCGATAGGTCAACGATTCTACTGCAGAACTTAGGTCTAGTTGTGACCTCTTTTATCATAGCATTTATACTCAACTGGCGTCTCACTCTTGTCGTCTTGGCCACATACCCATTGGTTATAAGCGGACATATCAGCGAGAAACTTTTCATGCAAGGATATGGAGGAGACTTGAACAAAGCATACTTAAAGGCCAACATGTTGGCCGGTGAGTCTGTTAGCAACATCCGCACGGTCGCTGCCTTCTGTGCTGAGGAAAAGATTCTAGAACTTTATTCAAGAGAGCTTTTAGAACCTTCCAAAAGTTCTTTCAGGCGTGGACAAATTGCAGGACTTTTCTACGGTGTCTCTCAGTTCTTCATTTTCTCTTCCTATGGCCTCGCCCTCTGGTACGGATCAACTTTGATGGACAAAGGATTAGCGGGCTTCAAATCAGTGATGAAAACATTCATGGTTTTGATTGTGACGGCGTTAGCGATGGGTGAGACATTGGCTCTAGCTCCGGATCTGCTGAAAGGAAACCAGATGGTTGCGTCCGTGTTTGAGATCTTGGACCGGAAAACTCAGATCGTTGGAGAAACCAGCGAGGAGCTGAACAATGTGGAAGGCACGATTGAGCTCAAAGGCGTCCACTTTAGCTACCCTTCGAGACCAGATGTTGTAATTTTCCGGGACTTTGATCTGATAGTTCGTGCCGGAAAGAGCATGGCGCTCGTTGGACAGAGTGGATCTGGGAAAAGTTCTGTTATTTCACTCATTCTCCGGTTCTATGACCCGACTGCTGGAAAAGTCATGATAGAGGGAAAAGACATCAAGAAACTAGATTTGAAAGCATTGAGGAAACACATTGGTCTTGTTCAACAAGAACCAGCACTTTTCGCCACAACAATCTACGAGAACATTTTGTATGGAAACGAAGGAGCATCTCAATCTGAAGTCGTCGAATCAGCTATGCTCGCAAATGCTCACAGCTTCATTACCTCTCTCCCAGAAGGTTACTCTACCAAAGTCGGTGAACGTGGAGTTCAAATGTCAGGCGGGCAGAGACAGAGGATCGCTATCGCTAGAGCCATTCTCAAGAATCCAGCAATTCTATTACTTGACGAAGCCACAAGCGCTTTGGACGTTGAATCTGAGCGTGTGGTACAACAAGCATTGGATCGGTTAATGGCTAACCGGACGACTGTGGTAGTTGCTCACAGGCTATCGACGATCAAGAACGCTGACACGATAAGTGTATTACACGGAGGTAAGATCGTAGAGCAAGGCAGCCACCGTAAGCTCGTTCTAAACAAGAGTGGTCCATATTTTAAACTTATCAGTCTTCAGCAGCAGCAACAACCTTAA

Protein Analysis

1273

Amino Acids

139.91

Weight (kDa)

9.06

Isoelectric Point (pI)

33.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane PF00664 82 - 349 2.3e-48 ABC transporter transmembrane region
ABC_tran PF00005 420 - 568 3.5e-33 ABC transporter
ABC_membrane PF00664 712 - 982 7.4e-47 ABC transporter transmembrane region
ABC_tran PF00005 1049 - 1197 1.5e-32 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0009779)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10680 AT1G10680 AT4G25960
fragaria_vesca FvH4_5g22520 FvH4_5g22520 FvH4_5g22520 FvH4_5g22520 FvH4_5g22520 FvH4_5g22520
malus_domestica MD06G1037400.v1.1
prunus_persica Prupe.5G031600_v2.0.a1
pyrus_communis pycom06g03090
rosa_chinensis RchiOBHm_Chr7g0210291
rosa_laevigata RLG00000003051 RLG00000029346
rosa_multiflora Rmu_sc0003639.1_g000002
rosa_roxburghii Rroxscaffold_3G00248450
rosa_rugosa Rorug07G0120500
rosa_samantha Rh7AG253400 Rh7CG270300
rosa_wichuraiana Rw7G021600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2597
AasI GACNNNNNNGTC 1 cut(s) 566
AatII GACGTC 1 cut(s) 2095
AccB1I GGYRCC 3 cut(s) 142, 263, 1072
AccBSI CCGCTC 1 cut(s) 1351
AccI GTMKAC 3 cut(s) 381, 1191, 2298
AccII CGCG 6 cut(s) 1217, 1990, 2183, 2276, 2358, 2440
AccIII TCCGGA 3 cut(s) 756, 2113, 2977
AclI AACGTT 1 cut(s) 1038
AcoI YGGCCR 3 cut(s) 1171, 2577, 2679
AcsI RAATTY 1 cut(s) 601
AcuI CTGAAG 3 cut(s) 1599, 3429, 3785
AcyI GRCGYC 7 cut(s) 143, 264, 1073, 2092, 2190, 2557, 3102
AfaI GTAC 7 cut(s) 365, 819, 887, 1095, 1830, 2867, 3621
AfeI AGCGCT 3 cut(s) 867, 1743, 3592
AfiI CCNNNNNNNGG 3 cut(s) 1246, 1370, 3257
AflII CTTAAG 1 cut(s) 188
AflIII ACRYGT 1 cut(s) 2673
AgeI ACCGGT 1 cut(s) 830
AhdI GACNNNNNGTC 1 cut(s) 1717
AhlI ACTAGT 1 cut(s) 653
AjuI GAANNNNNNNTTGG 2 cut(s) 2768, 2800
Alw21I GWGCWC 2 cut(s) 1331, 3096
AlwNI CAGNNNCTG 1 cut(s) 2019
Aor13HI TCCGGA 3 cut(s) 756, 2113, 2977
Aor51HI AGCGCT 3 cut(s) 867, 1743, 3592
AoxI GGCC 7 cut(s) 957, 1051, 1171, 2577, 2667, 2679, 2851
ApeKI GCWGC 9 cut(s) 124, 169, 1109, 1933, 2271, 2714, 3744, 3805, 3808
ApoI RAATTY 1 cut(s) 601
AsiGI ACCGGT 1 cut(s) 830
Asp700I GAANNNNTTC 3 cut(s) 2621, 2736, 2921
AspS9I GGNCC 5 cut(s) 813, 957, 988, 3028, 3776
AsuC2I CCSGG 2 cut(s) 1285, 3146
AvaII GGWCC 4 cut(s) 813, 988, 3028, 3776
BaeGI GKGCMC 1 cut(s) 1200
BalI TGGCCA 1 cut(s) 2579
BamHI GGATCC 1 cut(s) 239
BanI GGYRCC 3 cut(s) 142, 263, 1072
BanII GRGCYC 1 cut(s) 3096
BauI CACGAG 2 cut(s) 750, 1862
BbsI GAAGAC 1 cut(s) 3791
Bbv12I GWGCWC 2 cut(s) 1331, 3096
BbvCI CCTCAGC 2 cut(s) 1518, 2727
BbvI GCAGC 8 cut(s) 136, 181, 1096, 1945, 2258, 2701, 3756, 3817
BceAI ACGGC 3 cut(s) 112, 1158, 2958
BcnI CCSGG 2 cut(s) 1285, 3146
BcuI ACTAGT 1 cut(s) 653
BfmI CTRYAG 2 cut(s) 528, 2501
BfoI RGCGCY 7 cut(s) 146, 267, 869, 1076, 1745, 3188, 3594
BfrI CTTAAG 1 cut(s) 188
BglII AGATCT 1 cut(s) 3021
BlpI GCTNAGC 1 cut(s) 1961
BmcAI AGTACT 1 cut(s) 365
Bme1390I CCNGG 2 cut(s) 1285, 3146
Bme18I GGWCC 4 cut(s) 813, 988, 3028, 3776
BmeRI GACNNNNNGTC 1 cut(s) 1717
BmgT120I GGNCC 5 cut(s) 813, 957, 988, 3028, 3776
BmiI GGNNCC 6 cut(s) 144, 241, 265, 273, 1074, 1891
BmrFI CCNGG 2 cut(s) 1285, 3146
BmsI GCATC 7 cut(s) 423, 568, 726, 921, 2443, 2444, 3407
BpiI GAAGAC 1 cut(s) 3791
BplI GAGNNNNNCTC 6 cut(s) 462, 494, 1910, 1942, 3534, 3566
BpmI CTGGAG 2 cut(s) 890, 1392
Bpu10I CCTNAGC 2 cut(s) 1518, 2727
Bpu1102I GCTNAGC 1 cut(s) 1961
BpuEI CTTGAG 3 cut(s) 406, 2465, 3536
BpuMI CCSGG 2 cut(s) 1285, 3146
Bsa29I ATCGAT 2 cut(s) 929, 1676
BsaAI YACGTR 1 cut(s) 879
BsaBI GATNNNNATC 2 cut(s) 2258, 3533
BsaHI GRCGYC 7 cut(s) 143, 264, 1073, 2092, 2190, 2557, 3102
BsaI GGTCTC 1 cut(s) 3118
BsaJI CCNNGG 1 cut(s) 267
BsaWI WCCGGW 9 cut(s) 131, 756, 830, 2047, 2113, 2977, 3030, 3237, 3651
BsaXI ACNNNNNCTCC 4 cut(s) 863, 893, 3219, 3249
Bsc4I CCNNNNNNNGG 3 cut(s) 1246, 1370, 3257
Bse118I RCCGGY 5 cut(s) 621, 632, 830, 1049, 2681
Bse1I ACTGG 2 cut(s) 2123, 2558
Bse3DI GCAATG 1 cut(s) 1928
Bse8I GATNNNNATC 2 cut(s) 2258, 3533
BseAI TCCGGA 3 cut(s) 756, 2113, 2977
BseCI ATCGAT 2 cut(s) 929, 1676
BseDI CCNNGG 1 cut(s) 267
BseGI GGATG 7 cut(s) 438, 561, 2056, 2063, 2127, 2233, 2700
BseJI GATNNNNATC 2 cut(s) 2258, 3533
BseLI CCNNNNNNNGG 3 cut(s) 1246, 1370, 3257
BseMI GCAATG 1 cut(s) 1928
BseMII CTCAG 7 cut(s) 493, 1509, 1607, 2718, 2840, 3053, 3600
BseNI ACTGG 2 cut(s) 2123, 2558
BseRI GAGGAG 4 cut(s) 481, 1535, 2655, 3077
BseSI GKGCMC 1 cut(s) 1200
BseXI GCAGC 8 cut(s) 136, 181, 1096, 1945, 2258, 2701, 3756, 3817
Bsh1236I CGCG 6 cut(s) 1217, 1990, 2183, 2276, 2358, 2440
Bsh1285I CGRYCG 2 cut(s) 2048, 2712
BshFI GGCC 7 cut(s) 959, 1053, 1173, 2579, 2669, 2681, 2853
BshNI GGYRCC 3 cut(s) 142, 263, 1072
BshTI ACCGGT 1 cut(s) 830
BshVI ATCGAT 2 cut(s) 929, 1676
BsiEI CGRYCG 2 cut(s) 2048, 2712
BsiHKAI GWGCWC 2 cut(s) 1331, 3096
BslFI GGGAC 3 cut(s) 677, 2103, 3161
BslI CCNNNNNNNGG 3 cut(s) 1246, 1370, 3257
BsmBI CGTCTC 4 cut(s) 1916, 2197, 2345, 2564
BsmFI GGGAC 3 cut(s) 677, 2103, 3161
BsmI GAATGC 2 cut(s) 255, 824
BsnI GGCC 7 cut(s) 959, 1053, 1173, 2579, 2669, 2681, 2853
Bso31I GGTCTC 1 cut(s) 3118
Bsp1286I GDGCHC 3 cut(s) 1200, 1331, 3096
Bsp13I TCCGGA 3 cut(s) 756, 2113, 2977
Bsp1407I TGTACA 1 cut(s) 1828
Bsp1720I GCTNAGC 1 cut(s) 1961
BspANI GGCC 7 cut(s) 959, 1053, 1173, 2579, 2669, 2681, 2853
BspCNI CTCAG 7 cut(s) 492, 1510, 1606, 2719, 2839, 3052, 3601
BspDI ATCGAT 2 cut(s) 929, 1676
BspEI TCCGGA 3 cut(s) 756, 2113, 2977
BspFNI CGCG 6 cut(s) 1217, 1990, 2183, 2276, 2358, 2440
BspHI TCATGA 1 cut(s) 3267
BspLI GGNNCC 6 cut(s) 144, 241, 265, 273, 1074, 1891
BspMAI CTGCAG 1 cut(s) 2505
BspQI GCTCTTC 3 cut(s) 200, 1319, 1721
BspT107I GGYRCC 3 cut(s) 142, 263, 1072
BspTI CTTAAG 1 cut(s) 188
BspTNI GGTCTC 1 cut(s) 3118
BsrBI CCGCTC 1 cut(s) 1351
BsrDI GCAATG 1 cut(s) 1928
BsrFI RCCGGY 5 cut(s) 621, 632, 830, 1049, 2681
BsrGI TGTACA 1 cut(s) 1828
BsrI ACTGG 2 cut(s) 2123, 2558
BssAI RCCGGY 5 cut(s) 621, 632, 830, 1049, 2681
BssECI CCNNGG 1 cut(s) 267
BssNAI GTATAC 1 cut(s) 382
BssNI GRCGYC 7 cut(s) 143, 264, 1073, 2092, 2190, 2557, 3102
BssSI CACGAG 2 cut(s) 750, 1862
Bst1107I GTATAC 1 cut(s) 382
Bst2BI CACGAG 2 cut(s) 750, 1862
Bst6I CTCTTC 8 cut(s) 200, 786, 1319, 1721, 1903, 1908, 2270, 2848
BstACI GRCGYC 7 cut(s) 143, 264, 1073, 2092, 2190, 2557, 3102
BstAFI CTTAAG 1 cut(s) 188
BstAPI GCANNNNNTGC 1 cut(s) 1703
BstAUI TGTACA 1 cut(s) 1828
BstBAI YACGTR 1 cut(s) 879
BstF5I GGATG 7 cut(s) 438, 561, 2056, 2063, 2127, 2233, 2700
BstFNI CGCG 6 cut(s) 1217, 1990, 2183, 2276, 2358, 2440
BstH2I RGCGCY 7 cut(s) 146, 267, 869, 1076, 1745, 3188, 3594
BstMCI CGRYCG 2 cut(s) 2048, 2712
BstNSI RCATGY 2 cut(s) 429, 2677
BstSCI CCNGG 2 cut(s) 1283, 3144
BstSFI CTRYAG 2 cut(s) 528, 2501
BstSLI GKGCMC 1 cut(s) 1200
BstSNI TACGTA 1 cut(s) 879
BstUI CGCG 6 cut(s) 1217, 1990, 2183, 2276, 2358, 2440
BstV1I GCAGC 8 cut(s) 136, 181, 1096, 1945, 2258, 2701, 3756, 3817
BstV2I GAAGAC 1 cut(s) 3791
BstX2I RGATCY 6 cut(s) 239, 2009, 2162, 2980, 3021, 3203
BstXI CCANNNNNNTGG 1 cut(s) 2677
BstYI RGATCY 6 cut(s) 239, 2009, 2162, 2980, 3021, 3203
BstZ17I GTATAC 1 cut(s) 382
Bsu15I ATCGAT 2 cut(s) 929, 1676
BsuRI GGCC 7 cut(s) 959, 1053, 1173, 2579, 2669, 2681, 2853
BsuTUI ATCGAT 2 cut(s) 929, 1676
BtgZI GCGATG 4 cut(s) 1030, 2421, 2466, 2966
BtsCI GGATG 7 cut(s) 438, 561, 2056, 2063, 2127, 2233, 2700
BtsI GCAGTG 2 cut(s) 182, 1383
BtsIMutI CAGTG 6 cut(s) 182, 934, 1383, 1408, 2224, 2916
CaiI CAGNNNCTG 1 cut(s) 2019
CciI TCATGA 1 cut(s) 3267
Cfr10I RCCGGY 5 cut(s) 621, 632, 830, 1049, 2681
Cfr13I GGNCC 5 cut(s) 813, 957, 988, 3028, 3776
ClaI ATCGAT 2 cut(s) 929, 1676
Csp6I GTAC 7 cut(s) 364, 818, 886, 1094, 1829, 2866, 3620
CspAI ACCGGT 1 cut(s) 830
CspCI CAANNNNNGTGG 4 cut(s) 966, 1001, 1758, 1793
CviAII CATG 9 cut(s) 260, 411, 426, 1030, 2626, 2674, 2926, 3181, 3268
CviQI GTAC 7 cut(s) 364, 818, 886, 1094, 1829, 2866, 3620
DinI GGCGCC 3 cut(s) 144, 265, 1074
DraI TTTAAA 1 cut(s) 3787
DrdI GACNNNNNNGTC 1 cut(s) 566
DriI GACNNNNNGTC 1 cut(s) 1717
DseDI GACNNNNNNGTC 1 cut(s) 566
EaeI YGGCCR 3 cut(s) 1171, 2577, 2679
Eam1104I CTCTTC 8 cut(s) 200, 786, 1319, 1721, 1903, 1908, 2270, 2848
Eam1105I GACNNNNNGTC 1 cut(s) 1717
EarI CTCTTC 8 cut(s) 200, 786, 1319, 1721, 1903, 1908, 2270, 2848
EciI GGCGGA 2 cut(s) 535, 1641
Ecl136II GAGCTC 1 cut(s) 3094
Eco105I TACGTA 1 cut(s) 879
Eco24I GRGCYC 1 cut(s) 3096
Eco31I GGTCTC 1 cut(s) 3118
Eco32I GATATC 1 cut(s) 1000
Eco47I GGWCC 4 cut(s) 813, 988, 3028, 3776
Eco47III AGCGCT 3 cut(s) 867, 1743, 3592
Eco53kI GAGCTC 1 cut(s) 3094
Eco57I CTGAAG 3 cut(s) 1599, 3429, 3785
EcoICRI GAGCTC 1 cut(s) 3094
EcoRV GATATC 1 cut(s) 1000
EcoT22I ATGCAT 1 cut(s) 438
EcoT38I GRGCYC 1 cut(s) 3096
EgeI GGCGCC 3 cut(s) 144, 265, 1074
EheI GGCGCC 3 cut(s) 144, 265, 1074
Esp3I CGTCTC 4 cut(s) 1916, 2197, 2345, 2564
FaeI CATG 9 cut(s) 263, 414, 429, 1033, 2629, 2677, 2929, 3184, 3271
FaqI GGGAC 3 cut(s) 677, 2103, 3161
FatI CATG 9 cut(s) 259, 410, 425, 1029, 2625, 2673, 2925, 3180, 3267
FauI CCCGC 3 cut(s) 1983, 2890, 3506
FblI GTMKAC 3 cut(s) 381, 1191, 2298
FokI GGATG 7 cut(s) 445, 548, 2050, 2063, 2134, 2240, 2687
FriOI GRGCYC 1 cut(s) 3096
GsuI CTGGAG 2 cut(s) 890, 1392
HaeII RGCGCY 7 cut(s) 146, 267, 869, 1076, 1745, 3188, 3594
HaeIII GGCC 7 cut(s) 959, 1053, 1173, 2579, 2669, 2681, 2853
Hin1I GRCGYC 7 cut(s) 143, 264, 1073, 2092, 2190, 2557, 3102
Hin1II CATG 9 cut(s) 263, 414, 429, 1033, 2629, 2677, 2929, 3184, 3271
HincII GTYRAC 2 cut(s) 1447, 2490
HindII GTYRAC 2 cut(s) 1447, 2490
HindIII AAGCTT 2 cut(s) 505, 522
HpaI GTTAAC 1 cut(s) 1447
Hpy99I CGWCG 7 cut(s) 572, 734, 1511, 1694, 1889, 3419, 3688
HpyCH4IV ACGT 5 cut(s) 878, 1038, 2092, 3493, 3600
HpySE526I ACGT 5 cut(s) 878, 1038, 2092, 3493, 3600
Hsp92I GRCGYC 7 cut(s) 143, 264, 1073, 2092, 2190, 2557, 3102
Hsp92II CATG 9 cut(s) 263, 414, 429, 1033, 2629, 2677, 2929, 3184, 3271
KasI GGCGCC 3 cut(s) 142, 263, 1072
Kpn2I TCCGGA 3 cut(s) 756, 2113, 2977
KroI GCCGGC 1 cut(s) 1049
KroNI GCCGGC 1 cut(s) 1051
KspAI GTTAAC 1 cut(s) 1447
LguI GCTCTTC 3 cut(s) 200, 1319, 1721
LmnI GCTCC 6 cut(s) 248, 1373, 1889, 2980, 3064, 3395
Lsp1109I GCAGC 8 cut(s) 136, 181, 1096, 1945, 2258, 2701, 3756, 3817
LweI GCATC 7 cut(s) 423, 568, 726, 921, 2443, 2444, 3407
MaeII ACGT 5 cut(s) 878, 1038, 2092, 3493, 3600
MbiI CCGCTC 1 cut(s) 1351
MfeI CAATTG 2 cut(s) 642, 1659
MflI RGATCY 6 cut(s) 239, 2009, 2162, 2980, 3021, 3203
MhlI GDGCHC 3 cut(s) 1200, 1331, 3096
MlsI TGGCCA 1 cut(s) 2579
MluNI TGGCCA 1 cut(s) 2579
Mly113I GGCGCC 3 cut(s) 143, 264, 1073
MlyI GAGTC 7 cut(s) 226, 363, 1585, 1727, 1785, 2227, 2696
MmeI TCCRAC 8 cut(s) 113, 351, 410, 582, 1285, 1701, 3028, 3172
Mox20I TGGCCA 1 cut(s) 2579
Mph1103I ATGCAT 1 cut(s) 438
MroI TCCGGA 3 cut(s) 756, 2113, 2977
MroNI GCCGGC 1 cut(s) 1049
MroXI GAANNNNTTC 3 cut(s) 2621, 2736, 2921
MscI TGGCCA 1 cut(s) 2579
MslI CAYNNNNRTG 1 cut(s) 1028
Msp20I TGGCCA 1 cut(s) 2579
MspA1I CMGCKG 1 cut(s) 1109
MspCI CTTAAG 1 cut(s) 188
MspR9I CCNGG 2 cut(s) 1285, 3146
MunI CAATTG 2 cut(s) 642, 1659
Mva1269I GAATGC 2 cut(s) 255, 824
MvnI CGCG 6 cut(s) 1217, 1990, 2183, 2276, 2358, 2440
NaeI GCCGGC 1 cut(s) 1051
NarI GGCGCC 3 cut(s) 143, 264, 1073
NciI CCSGG 2 cut(s) 1285, 3146
NgoMIV GCCGGC 1 cut(s) 1049
NlaIII CATG 9 cut(s) 263, 414, 429, 1033, 2629, 2677, 2929, 3184, 3271
NlaIV GGNNCC 6 cut(s) 144, 241, 265, 273, 1074, 1891
NmuCI GTSAC 5 cut(s) 180, 676, 1147, 2521, 2938
NsiI ATGCAT 1 cut(s) 438
NspI RCATGY 2 cut(s) 429, 2677
PagI TCATGA 1 cut(s) 3267
PciI ACATGT 1 cut(s) 2673
PciSI GCTCTTC 3 cut(s) 200, 1319, 1721
PcsI WCGNNNNNNNCGW 2 cut(s) 2187, 2948
PctI GAATGC 2 cut(s) 255, 824
PdiI GCCGGC 1 cut(s) 1051
PdmI GAANNNNTTC 3 cut(s) 2621, 2736, 2921
PfoI TCCNGGA 1 cut(s) 3144
PinAI ACCGGT 1 cut(s) 830
PleI GAGTC 7 cut(s) 226, 362, 1585, 1726, 1785, 2227, 2695
PluTI GGCGCC 3 cut(s) 146, 267, 1076
PpsI GAGTC 7 cut(s) 226, 362, 1585, 1726, 1785, 2227, 2695
Ppu21I YACGTR 1 cut(s) 879
PscI ACATGT 1 cut(s) 2673
PsiI TTATAA 1 cut(s) 2597
Psp124BI GAGCTC 1 cut(s) 3096
Psp1406I AACGTT 1 cut(s) 1038
PspN4I GGNNCC 6 cut(s) 144, 241, 265, 273, 1074, 1891
PspPI GGNCC 5 cut(s) 813, 957, 988, 3028, 3776
PsrI GAACNNNNNNTAC 2 cut(s) 2642, 2674
PstI CTGCAG 1 cut(s) 2505
PstNI CAGNNNCTG 1 cut(s) 2019
PsuI RGATCY 6 cut(s) 239, 2009, 2162, 2980, 3021, 3203
RsaI GTAC 7 cut(s) 365, 819, 887, 1095, 1830, 2867, 3621
RsaNI GTAC 7 cut(s) 364, 818, 886, 1094, 1829, 2866, 3620
RseI CAYNNNNRTG 1 cut(s) 1028
SacI GAGCTC 1 cut(s) 3096
SapI GCTCTTC 3 cut(s) 200, 1319, 1721
Sau96I GGNCC 5 cut(s) 813, 957, 988, 3028, 3776
ScaI AGTACT 1 cut(s) 365
SchI GAGTC 7 cut(s) 226, 363, 1585, 1727, 1785, 2227, 2696
ScrFI CCNGG 2 cut(s) 1285, 3146
SduI GDGCHC 3 cut(s) 1200, 1331, 3096
SfaNI GCATC 7 cut(s) 423, 568, 726, 921, 2443, 2444, 3407
SfcI CTRYAG 2 cut(s) 528, 2501
SfoI GGCGCC 3 cut(s) 144, 265, 1074
SgrAI CRCCGGYG 1 cut(s) 830
SinI GGWCC 4 cut(s) 813, 988, 3028, 3776
SmiMI CAYNNNNRTG 1 cut(s) 1028
SmlI CTYRAG 4 cut(s) 188, 385, 2444, 3551
SmoI CTYRAG 4 cut(s) 188, 385, 2444, 3551
SnaBI TACGTA 1 cut(s) 879
SpeI ACTAGT 1 cut(s) 653
SspDI GGCGCC 3 cut(s) 142, 263, 1072
SspI AATATT 1 cut(s) 310
SstI GAGCTC 1 cut(s) 3096
StyD4I CCNGG 2 cut(s) 1283, 3144
TaiI ACGT 5 cut(s) 881, 1041, 2095, 3496, 3603
TaqII GACCGA 1 cut(s) 305
TatI WGTACW 2 cut(s) 363, 1828
TauI GCSGC 6 cut(s) 458, 717, 1073, 1109, 1538, 2443
TscAI CASTG 6 cut(s) 189, 941, 1383, 1408, 2224, 2916
TseFI GTSAC 5 cut(s) 180, 676, 1147, 2521, 2938
TseI GCWGC 9 cut(s) 124, 169, 1109, 1933, 2271, 2714, 3744, 3805, 3808
Tsp45I GTSAC 5 cut(s) 180, 676, 1147, 2521, 2938
TspGWI ACGGA 9 cut(s) 232, 1153, 1902, 2099, 2269, 2339, 2883, 3001, 3735
TspRI CASTG 6 cut(s) 189, 941, 1383, 1408, 2224, 2916
Vha464I CTTAAG 1 cut(s) 188
VpaK11BI GGWCC 4 cut(s) 813, 988, 3028, 3776
XapI RAATTY 1 cut(s) 601
XbaI TCTAGA 1 cut(s) 2740
XceI RCATGY 2 cut(s) 429, 2677
XcmI CCANNNNNNNNNTGG 3 cut(s) 367, 2587, 3592
XmiI GTMKAC 3 cut(s) 381, 1191, 2298
XmnI GAANNNNTTC 3 cut(s) 2621, 2736, 2921
ZraI GACGTC 1 cut(s) 2093
ZrmI AGTACT 1 cut(s) 365
Zsp2I ATGCAT 1 cut(s) 438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.