AT1G12150

WEB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0011447
Physical Location & Seq
Reverse (-)
4123530 .. 4125651
2122 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G12150.2

Sequence Viewer

Length: 1647 bp
ATGGTGAACATTAGAGTACAAAAAGCTCCGGAATCTCCGAGGACGATGGAGGTGGGAGAAATAGACACAAGAGCACCGTTCCAATCGGTGAAAGCTGCGGTGAGCTTGTTCGGAGAAGTTGCAGTCTCAAAGCAAAGAAGTACTCCAAGGCGATCTCGACTCTCCTCTGAGAGCGTTTGCGACAAGGAAACACAGCTAATGCTGGTCCACAAGGAATTCATGAAGATCAAGCAAAAGCTTGACAACGCTGAGAGCACAAGATCTCGTGCTCTAGACGATCTCTCAAAGGCGAAGAAGACAATGGAGGATCTTAGCAACAAACTTGAGACTGTCAACAAGTCTAAGCAATCAGCTATTGACACCAAAGAAACTGTCCAACAGCGGGAAGAACAGCTTGAGCATGATAAGTGTCATGGCTCTCCTCCTCATCACCATGAACTTGACGTCGCTAGAGAACAATATATCTCCACCACCGTTGAACTTGATGCTGCAAAACAGCAGCTCAACAAAATTAGACAGAGCTTTGATTCCGCTATGGATTTCAAAGCCACGGCCTTAAACCAGGCTGCAGAAGCTCAACGTGCACTCCAAGTCAACTCCGCCAAAGTTAACGAGCTTTCTAAGGAGATTTCGGATATGAAAGATGCGATTCATCAGCTCAAGCTAGCGGCTGCTCAGAATCTGCAGGAGCACGCTAATATTGTTAAGGAAAAAGACGATTTACGAGAATGTTATAGAACTGCTGTTGAAGAGGCAGAGAAGAAACTGCTAGTGTTGAGGAAAGAATATGAGCCTGAGCTCTCAAGAACTCTTGAAGCCAAGCTGTTAGAGACAACTTCAGAGATTGAGGTTTTACGGGAAGAGATGAAGAAAGCTCATGAATCAGAGATGAACACGGTTAAGATCATTACAAATGAGCTTAATGAAGCAACGATGAGGCTACAAGAAGCTGCTGATGACGAATGCTCTCTCCGAAGTTTGGTGAATTCTCTCAGGATGGAACTAGAAGACTTGAGAAGAGAACGCGAAGAGCTGCAGCAGAAAGAAGCAGAGAGATTAGAGATTGAGGAAACAAAAAAGTTGGAAGCTCTCAAGCAAGAGAGCTTGAAACTGGAGCAAATGAAAACAGAAGCTATTGAAGCAAGAAACGAAGCTGCAAATATGAATAGGAAGATAGAGAGTCTGAAGAAAGAAACAGAGGCCGCGATGATAGCTGCTGAGGAAGCTGAAAAGAGACTAGAGCTTGTTATAAGAGAAGTGGAGGAGGCGAAATCCGCTGAAGAGAAAGTCCGCGAAGAGATGAAGATGATATCTCAGAAGCAAGAGAGCAAGAAACAAGATGAAGAATCATCTGGTTCAAAGATAAAAATCACAATTCAAGAGTTTGAATCTTTGAAACGAGGAGCAGGGGAAACAGAGGCTGCAATCGAAAAGAAGTTGGCAACTATAGCAGCTGAGCTTGAAGAGATCAACAAACGAAGAGCCGAAGCGGATAATAAGTTAGAAGCGAACCTGAAAGCGATAGAGGAGATGAAGCAAGCAACGGAATTGGCTCAGAAGTCAGCAGAATCTGCAGAAGCTGCAAAGAGAATGGTGGAAAGTGAGTTGCAGAGATGGCGTCAACAAGAAAATGTACAACTTGCTTAG

Protein Analysis

548

Amino Acids

62.65

Weight (kDa)

5.37

Isoelectric Point (pI)

62.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WEMBL PF05701 20 - 544 5.2e-31 Weak chloroplast movement under blue light
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011447)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12150 AT1G12150
fragaria_vesca FvH4_3g00281 FvH4_3g00281
malus_domestica MD05G1362200.v1.1 MD10G1339700.v1.1
prunus_persica Prupe.4G003200_v2.0.a1
pyrus_communis pycom05g32850 pycom05g32860 pycom10g28950
rosa_chinensis RchiOBHm_Chr5g0000421
rosa_laevigata RLG00000030865
rosa_multiflora Rmu_co8243975.1_g000001
rosa_roxburghii Rroxscaffold_1G00075740
rosa_rugosa Rorug04G0383500
rosa_samantha Rh5AG003400 Rh5BG004500 Rh5CG003600 Rh5DG003500
rosa_wichuraiana Rw5G000340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1250
AatII GACGTC 1 cut(s) 447
AccII CGCG 3 cut(s) 1026, 1205, 1293
AccIII TCCGGA 1 cut(s) 28
AciI CCGC 9 cut(s) 98, 382, 531, 600, 668, 1203, 1275, 1291, 1490
AclWI GGATC 1 cut(s) 315
AcsI RAATTY 2 cut(s) 215, 985
AcuI CTGAAG 3 cut(s) 822, 1205, 1299
AcyI GRCGYC 2 cut(s) 444, 1618
AfaI GTAC 3 cut(s) 18, 142, 1635
AfiI CCNNNNNNNGG 2 cut(s) 382, 979
AjnI CCWGG 1 cut(s) 561
Alw21I GWGCWC 6 cut(s) 76, 257, 271, 586, 693, 801
Alw26I GTCTC 4 cut(s) 130, 320, 824, 1228
Alw44I GTGCAC 1 cut(s) 582
AlwI GGATC 1 cut(s) 315
AlwNI CAGNNNCTG 4 cut(s) 682, 1421, 1571, 1580
Aor13HI TCCGGA 1 cut(s) 28
AoxI GGCC 2 cut(s) 552, 1200
ApaLI GTGCAC 1 cut(s) 582
ApoI RAATTY 2 cut(s) 215, 985
ArsI GACNNNNNNTTYG 2 cut(s) 357, 389
AspS9I GGNCC 1 cut(s) 205
AsuHPI GGTGA 5 cut(s) 16, 100, 112, 422, 994
AsuNHI GCTAGC 1 cut(s) 664
AvaII GGWCC 1 cut(s) 205
BaeGI GKGCMC 1 cut(s) 586
BanII GRGCYC 1 cut(s) 801
BauI CACGAG 1 cut(s) 264
BbsI GAAGAC 2 cut(s) 302, 1014
Bbv12I GWGCWC 6 cut(s) 76, 257, 271, 586, 693, 801
BbvCI CCTCAGC 1 cut(s) 1218
BccI CCATC 3 cut(s) 40, 991, 1608
BceAI ACGGC 1 cut(s) 567
BciT130I CCWGG 1 cut(s) 563
BcoDI GTCTC 4 cut(s) 130, 320, 824, 1228
BfaI CTAG 6 cut(s) 272, 450, 665, 770, 1004, 1238
BfmI CTRYAG 5 cut(s) 567, 683, 1034, 1446, 1572
BglII AGATCT 1 cut(s) 260
BlpI GCTNAGC 1 cut(s) 1455
BmcAI AGTACT 1 cut(s) 142
Bme1390I CCNGG 1 cut(s) 563
Bme18I GGWCC 1 cut(s) 205
BmgT120I GGNCC 1 cut(s) 205
BmrFI CCNGG 1 cut(s) 563
BmsI GCATC 2 cut(s) 475, 634
BmtI GCTAGC 1 cut(s) 668
BpiI GAAGAC 2 cut(s) 302, 1014
BpmI CTGGAG 1 cut(s) 1133
Bpu10I CCTNAGC 2 cut(s) 795, 1218
Bpu1102I GCTNAGC 1 cut(s) 1455
BpuEI CTTGAG 6 cut(s) 344, 416, 644, 787, 1033, 1076
BsaBI GATNNNNATC 1 cut(s) 1367
BsaHI GRCGYC 2 cut(s) 444, 1618
BsaJI CCNNGG 3 cut(s) 38, 146, 549
BsaWI WCCGGW 1 cut(s) 28
BsaXI ACNNNNNCTCC 2 cut(s) 570, 600
Bsc4I CCNNNNNNNGG 2 cut(s) 382, 979
Bse1I ACTGG 1 cut(s) 1116
Bse8I GATNNNNATC 1 cut(s) 1367
BseAI TCCGGA 1 cut(s) 28
BseBI CCWGG 1 cut(s) 563
BseDI CCNNGG 3 cut(s) 38, 146, 549
BseGI GGATG 1 cut(s) 1002
BseJI GATNNNNATC 1 cut(s) 1367
BseLI CCNNNNNNNGG 2 cut(s) 382, 979
BseMII CTCAG 9 cut(s) 159, 240, 689, 786, 1006, 1209, 1328, 1446, 1568
BseNI ACTGG 1 cut(s) 1116
BseRI GAGGAG 6 cut(s) 154, 411, 414, 1277, 1416, 1541
BseSI GKGCMC 1 cut(s) 586
Bsh1236I CGCG 3 cut(s) 1026, 1205, 1293
BshFI GGCC 2 cut(s) 554, 1202
BsiHKAI GWGCWC 6 cut(s) 76, 257, 271, 586, 693, 801
BsiSI CCGG 1 cut(s) 29
BslI CCNNNNNNNGG 2 cut(s) 382, 979
BsmAI GTCTC 4 cut(s) 130, 320, 824, 1228
BsmI GAATGC 1 cut(s) 968
BsnI GGCC 2 cut(s) 554, 1202
Bsp1286I GDGCHC 6 cut(s) 76, 257, 271, 586, 693, 801
Bsp13I TCCGGA 1 cut(s) 28
Bsp1407I TGTACA 1 cut(s) 1633
Bsp143I GATC 7 cut(s) 152, 225, 260, 277, 307, 903, 1467
Bsp1720I GCTNAGC 1 cut(s) 1455
BspACI CCGC 9 cut(s) 98, 382, 531, 600, 668, 1203, 1275, 1291, 1490
BspANI GGCC 2 cut(s) 554, 1202
BspCNI CTCAG 9 cut(s) 160, 241, 688, 787, 1005, 1210, 1327, 1447, 1567
BspEI TCCGGA 1 cut(s) 28
BspFNI CGCG 3 cut(s) 1026, 1205, 1293
BspHI TCATGA 2 cut(s) 219, 877
BspMAI CTGCAG 4 cut(s) 571, 687, 1038, 1576
BspOI GCTAGC 1 cut(s) 668
BspPI GGATC 1 cut(s) 315
BspQI GCTCTTC 2 cut(s) 1023, 1474
BsrGI TGTACA 1 cut(s) 1633
BsrI ACTGG 1 cut(s) 1116
BssECI CCNNGG 3 cut(s) 38, 146, 549
BssMI GATC 7 cut(s) 152, 225, 260, 277, 307, 903, 1467
BssNI GRCGYC 2 cut(s) 444, 1618
BssSI CACGAG 1 cut(s) 264
BssT1I CCWWGG 1 cut(s) 146
Bst2BI CACGAG 1 cut(s) 264
Bst2UI CCWGG 1 cut(s) 563
Bst4CI ACNGT 5 cut(s) 78, 331, 373, 475, 898
Bst6I CTCTTC 8 cut(s) 744, 855, 1012, 1023, 1275, 1290, 1458, 1474
BstACI GRCGYC 2 cut(s) 444, 1618
BstAPI GCANNNNNTGC 2 cut(s) 1571, 1580
BstAUI TGTACA 1 cut(s) 1633
BstC8I GCNNGC 3 cut(s) 666, 693, 1539
BstDSI CCRYGG 1 cut(s) 549
BstF5I GGATG 1 cut(s) 1002
BstFNI CGCG 3 cut(s) 1026, 1205, 1293
BstKTI GATC 7 cut(s) 155, 228, 263, 280, 310, 906, 1470
BstMAI GTCTC 4 cut(s) 130, 320, 824, 1228
BstMBI GATC 7 cut(s) 152, 225, 260, 277, 307, 903, 1467
BstNI CCWGG 1 cut(s) 563
BstSCI CCNGG 1 cut(s) 561
BstSFI CTRYAG 5 cut(s) 567, 683, 1034, 1446, 1572
BstSLI GKGCMC 1 cut(s) 586
BstUI CGCG 3 cut(s) 1026, 1205, 1293
BstV2I GAAGAC 2 cut(s) 302, 1014
BstX2I RGATCY 2 cut(s) 260, 307
BstYI RGATCY 2 cut(s) 260, 307
BsuRI GGCC 2 cut(s) 554, 1202
BtgI CCRYGG 1 cut(s) 549
BtgZI GCGATG 1 cut(s) 1220
BtsCI GGATG 1 cut(s) 1002
Cac8I GCNNGC 3 cut(s) 666, 693, 1539
CaiI CAGNNNCTG 4 cut(s) 682, 1421, 1571, 1580
CciI TCATGA 2 cut(s) 219, 877
Cfr13I GGNCC 1 cut(s) 205
CseI GACGC 1 cut(s) 1607
Csp6I GTAC 3 cut(s) 17, 141, 1634
CspCI CAANNNNNGTGG 2 cut(s) 457, 492
CviAII CATG 5 cut(s) 220, 401, 413, 434, 878
CviQI GTAC 3 cut(s) 17, 141, 1634
DpnI GATC 7 cut(s) 154, 227, 262, 279, 309, 905, 1469
DpnII GATC 7 cut(s) 152, 225, 260, 277, 307, 903, 1467
Eam1104I CTCTTC 8 cut(s) 744, 855, 1012, 1023, 1275, 1290, 1458, 1474
EarI CTCTTC 8 cut(s) 744, 855, 1012, 1023, 1275, 1290, 1458, 1474
EciI GGCGGA 1 cut(s) 589
Ecl136II GAGCTC 1 cut(s) 799
Eco130I CCWWGG 1 cut(s) 146
Eco24I GRGCYC 1 cut(s) 801
Eco32I GATATC 1 cut(s) 1311
Eco47I GGWCC 1 cut(s) 205
Eco53kI GAGCTC 1 cut(s) 799
Eco57I CTGAAG 3 cut(s) 822, 1205, 1299
EcoICRI GAGCTC 1 cut(s) 799
EcoRI GAATTC 2 cut(s) 215, 985
EcoRII CCWGG 1 cut(s) 561
EcoRV GATATC 1 cut(s) 1311
EcoT14I CCWWGG 1 cut(s) 146
EcoT38I GRGCYC 1 cut(s) 801
ErhI CCWWGG 1 cut(s) 146
FaeI CATG 5 cut(s) 223, 404, 416, 437, 881
FalI AAGNNNNNCTT 2 cut(s) 378, 410
FatI CATG 5 cut(s) 219, 400, 412, 433, 877
FauI CCCGC 1 cut(s) 375
FokI GGATG 1 cut(s) 1009
FriOI GRGCYC 1 cut(s) 801
FspBI CTAG 6 cut(s) 272, 450, 665, 770, 1004, 1238
GsuI CTGGAG 1 cut(s) 1133
HaeIII GGCC 2 cut(s) 554, 1202
HapII CCGG 1 cut(s) 29
HgaI GACGC 1 cut(s) 1607
Hin1I GRCGYC 2 cut(s) 444, 1618
Hin1II CATG 5 cut(s) 223, 404, 416, 437, 881
HincII GTYRAC 4 cut(s) 334, 595, 610, 1622
HindII GTYRAC 4 cut(s) 334, 595, 610, 1622
HindIII AAGCTT 1 cut(s) 236
HpaI GTTAAC 1 cut(s) 610
HpaII CCGG 1 cut(s) 29
HphI GGTGA 5 cut(s) 16, 100, 112, 422, 994
Hpy166II GTNNAC 7 cut(s) 7, 208, 334, 584, 595, 610, 1622
Hpy188III TCNNGA 9 cut(s) 29, 156, 220, 272, 804, 812, 878, 994, 1379
Hpy8I GTNNAC 7 cut(s) 7, 208, 334, 584, 595, 610, 1622
Hpy99I CGWCG 1 cut(s) 449
HpyCH4III ACNGT 5 cut(s) 78, 331, 373, 475, 898
HpyCH4IV ACGT 2 cut(s) 444, 580
HpySE526I ACGT 2 cut(s) 444, 580
Hsp92I GRCGYC 2 cut(s) 444, 1618
Hsp92II CATG 5 cut(s) 223, 404, 416, 437, 881
Kpn2I TCCGGA 1 cut(s) 28
KspAI GTTAAC 1 cut(s) 610
Kzo9I GATC 7 cut(s) 152, 225, 260, 277, 307, 903, 1467
LguI GCTCTTC 2 cut(s) 1023, 1474
LmnI GCTCC 4 cut(s) 31, 688, 1114, 1403
LweI GCATC 2 cut(s) 475, 634
MaeI CTAG 6 cut(s) 272, 450, 665, 770, 1004, 1238
MaeII ACGT 2 cut(s) 444, 580
MalI GATC 7 cut(s) 154, 227, 262, 279, 309, 905, 1469
MboI GATC 7 cut(s) 152, 225, 260, 277, 307, 903, 1467
MflI RGATCY 2 cut(s) 260, 307
MhlI GDGCHC 6 cut(s) 76, 257, 271, 586, 693, 801
MluCI AATT 5 cut(s) 215, 510, 985, 1374, 1547
MlyI GAGTC 2 cut(s) 153, 1189
MmeI TCCRAC 2 cut(s) 400, 1062
MroI TCCGGA 1 cut(s) 28
MseI TTAA 5 cut(s) 557, 609, 705, 900, 921
MslI CAYNNNNRTG 1 cut(s) 432
MspA1I CMGCKG 3 cut(s) 382, 1277, 1454
MspI CCGG 1 cut(s) 29
MspR9I CCNGG 1 cut(s) 563
Mva1269I GAATGC 1 cut(s) 968
MvaI CCWGG 1 cut(s) 563
MvnI CGCG 3 cut(s) 1026, 1205, 1293
NdeII GATC 7 cut(s) 152, 225, 260, 277, 307, 903, 1467
NheI GCTAGC 1 cut(s) 664
NlaIII CATG 5 cut(s) 223, 404, 416, 437, 881
PagI TCATGA 2 cut(s) 219, 877
PciSI GCTCTTC 2 cut(s) 1023, 1474
PctI GAATGC 1 cut(s) 968
PfeI GAWTC 8 cut(s) 32, 527, 649, 679, 881, 1346, 1388, 1568
PleI GAGTC 2 cut(s) 153, 1188
PpsI GAGTC 2 cut(s) 153, 1188
PsiI TTATAA 1 cut(s) 1250
Psp124BI GAGCTC 1 cut(s) 801
Psp6I CCWGG 1 cut(s) 561
PspGI CCWGG 1 cut(s) 561
PspPI GGNCC 1 cut(s) 205
PstI CTGCAG 4 cut(s) 571, 687, 1038, 1576
PstNI CAGNNNCTG 4 cut(s) 682, 1421, 1571, 1580
PsuI RGATCY 2 cut(s) 260, 307
PvuII CAGCTG 1 cut(s) 1454
RsaI GTAC 3 cut(s) 18, 142, 1635
RsaNI GTAC 3 cut(s) 17, 141, 1634
RseI CAYNNNNRTG 1 cut(s) 432
SacI GAGCTC 1 cut(s) 801
SapI GCTCTTC 2 cut(s) 1023, 1474
SaqAI TTAA 5 cut(s) 557, 609, 705, 900, 921
Sau3AI GATC 7 cut(s) 152, 225, 260, 277, 307, 903, 1467
Sau96I GGNCC 1 cut(s) 205
ScaI AGTACT 1 cut(s) 142
SchI GAGTC 2 cut(s) 153, 1189
ScrFI CCNGG 1 cut(s) 563
SduI GDGCHC 6 cut(s) 76, 257, 271, 586, 693, 801
SfaNI GCATC 2 cut(s) 475, 634
SfcI CTRYAG 5 cut(s) 567, 683, 1034, 1446, 1572
SinI GGWCC 1 cut(s) 205
SmiMI CAYNNNNRTG 1 cut(s) 432
SmlI CTYRAG 6 cut(s) 323, 395, 659, 802, 1012, 1091
SmoI CTYRAG 6 cut(s) 323, 395, 659, 802, 1012, 1091
Sse9I AATT 5 cut(s) 215, 510, 985, 1374, 1547
SsiI CCGC 9 cut(s) 98, 382, 531, 600, 668, 1203, 1275, 1291, 1490
SspI AATATT 1 cut(s) 700
SspMI CTAG 6 cut(s) 272, 450, 665, 770, 1004, 1238
SstI GAGCTC 1 cut(s) 801
StyD4I CCNGG 1 cut(s) 561
StyI CCWWGG 1 cut(s) 146
TaaI ACNGT 5 cut(s) 78, 331, 373, 475, 898
TaiI ACGT 2 cut(s) 447, 583
TaqI TCGA 2 cut(s) 157, 1428
TasI AATT 5 cut(s) 215, 510, 985, 1374, 1547
TatI WGTACW 3 cut(s) 16, 140, 1633
TauI GCSGC 2 cut(s) 671, 1205
TfiI GAWTC 8 cut(s) 32, 527, 649, 679, 881, 1346, 1388, 1568
Tru1I TTAA 5 cut(s) 557, 609, 705, 900, 921
Tru9I TTAA 5 cut(s) 557, 609, 705, 900, 921
TspGWI ACGGA 1 cut(s) 1559
VneI GTGCAC 1 cut(s) 582
VpaK11BI GGWCC 1 cut(s) 205
XapI RAATTY 2 cut(s) 215, 985
XbaI TCTAGA 1 cut(s) 271
XspI CTAG 6 cut(s) 272, 450, 665, 770, 1004, 1238
ZraI GACGTC 1 cut(s) 445
ZrmI AGTACT 1 cut(s) 142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.