pycom05g32850

WEB family protein At1g12150-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0011447
Physical Location & Seq
Forward (+)
32100675 .. 32101747
1073 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g32850.1

Sequence Viewer

Length: 969 bp
ATGCATGCGTGTTGCGCGCAGAAGAAACCAAAAATGGCGAATGTCCGTATAAAGGATCAGCAGAAGGCTCCCGGCTCCCCGAGGGCGGAGGTGGGAGAGGTTGACACCCGAGCACCCTTCCAATCTGTCAAAGCTGCTGTTAGTTTGTTTGGCCAAGTCGTTTCTAGAGGAGGAAAATCTTCCAATGCCAATGCCAATTCCGATTCCAATTCCAGTGCCAGCCCCAAAGCCAATGCTAATGCCAACGCCAATGCCAATGCCAATGCCGTCAAGAAAAAGCTTTCGCTAACGTCTTGCGAAGAGAACAGCCTTTCTATTTTTGTATTGCGTATATCATCTCTTCTGAATGATTTGATATTTAAGTGGGGATTTCTTGTTTGTCAGACTAATGCACTGGACAAGGAGACGCAACTTCTGTTGGCACAGAAAGAACTAAACAAGATTAAGCAACAATTAGGGAGTGCAGAAACTACCAAAGCTAGAGCACTCTCTGAGCTTGATAAGGCCAAGAGAACACTGCAGGATCTGAACACCAAGCTCAAAACTGTAACAGACTCCAAGCGATCAGCAATTAAAGACGCCGAAGATGTAAAGAATCGGGCTAAGAAACTAGAAGAGGTCAAATCCAGAGAAACCATTGAAGGTAATGCTTGGAAACGGGAATTGGACCACGAAAGAAATGAATACACAGCCACTGTGACCGAACTCGATGCAGCAAAGCAAGAGCTTACCAAAATCCGACAGGACTTTGACGCCGCCTTTGAAGCAAAACTGGCTGCATTCCAACAGGCGGCGGAAGCTCAGCGGTCGGCAAACGTTAACTCAGACAGGGTTAACGAGCTGTCGAAGGAGGTTTCAGCCATGCAGGGATCAATTGAACAGCTCAAACTTGCTGCCCAGCAAGCCCAACAGGAGCAGGCAAAAGCCGTGTCCGAAAAAGAGGCCCACCTTCGATCCTACCGAAGTTGA

Protein Analysis

323

Amino Acids

35.35

Weight (kDa)

9.38

Isoelectric Point (pI)

42.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WEMBL PF05701 137 - 312 2e-15 Weak chloroplast movement under blue light
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011447)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12150 AT1G12150
fragaria_vesca FvH4_3g00281 FvH4_3g00281
malus_domestica MD05G1362200.v1.1 MD10G1339700.v1.1
prunus_persica Prupe.4G003200_v2.0.a1
pyrus_communis pycom05g32850 pycom05g32860 pycom10g28950
rosa_chinensis RchiOBHm_Chr5g0000421
rosa_laevigata RLG00000030865
rosa_multiflora Rmu_co8243975.1_g000001
rosa_roxburghii Rroxscaffold_1G00075740
rosa_rugosa Rorug04G0383500
rosa_samantha Rh5AG003400 Rh5BG004500 Rh5CG003600 Rh5DG003500
rosa_wichuraiana Rw5G000340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 17
AciI CCGC 5 cut(s) 86, 756, 791, 794, 805
AclI AACGTT 1 cut(s) 816
AclWI GGATC 4 cut(s) 63, 531, 877, 948
AcoI YGGCCR 1 cut(s) 151
AcyI GRCGYC 2 cut(s) 579, 753
AfiI CCNNNNNNNGG 3 cut(s) 52, 85, 790
AgsI TTSAA 3 cut(s) 641, 764, 878
AjuI GAANNNNNNNTTGG 2 cut(s) 647, 679
AluBI AGCT 9 cut(s) 134, 280, 479, 496, 538, 727, 800, 841, 883
AluI AGCT 9 cut(s) 134, 280, 479, 496, 538, 727, 800, 841, 883
Alw21I GWGCWC 2 cut(s) 115, 487
Alw26I GTCTC 1 cut(s) 398
AlwI GGATC 4 cut(s) 63, 531, 877, 948
AlwNI CAGNNNCTG 2 cut(s) 526, 695
Ama87I CYCGRG 2 cut(s) 79, 108
AoxI GGCC 3 cut(s) 151, 504, 942
ApeKI GCWGC 4 cut(s) 134, 713, 776, 893
ArsI GACNNNNNNTTYG 4 cut(s) 743, 775, 914, 946
Asp700I GAANNNNTTC 1 cut(s) 178
AspLEI GCGC 2 cut(s) 17, 19
AspS9I GGNCC 2 cut(s) 667, 943
AsuC2I CCSGG 1 cut(s) 72
AvaI CYCGRG 2 cut(s) 79, 108
AvaII GGWCC 1 cut(s) 667
BalI TGGCCA 1 cut(s) 153
Bbv12I GWGCWC 2 cut(s) 115, 487
BbvI GCAGC 4 cut(s) 121, 725, 763, 880
BceAI ACGGC 2 cut(s) 251, 911
BcgI CGANNNNNNTGC 2 cut(s) 692, 726
BcnI CCSGG 1 cut(s) 72
BcoDI GTCTC 1 cut(s) 398
BfaI CTAG 3 cut(s) 165, 480, 611
BfmI CTRYAG 1 cut(s) 518
BisI GCNGC 6 cut(s) 135, 714, 756, 777, 792, 894
BlpI GCTNAGC 1 cut(s) 801
BlsI GCNGC 6 cut(s) 136, 715, 757, 778, 793, 895
Bme1390I CCNGG 1 cut(s) 72
Bme18I GGWCC 1 cut(s) 667
BmeT110I CYCGRG 2 cut(s) 79, 108
BmgT120I GGNCC 2 cut(s) 667, 943
BmiI GGNNCC 2 cut(s) 69, 76
BmrFI CCNGG 1 cut(s) 72
BmsI GCATC 1 cut(s) 700
Bpu1102I GCTNAGC 1 cut(s) 801
BpuMI CCSGG 1 cut(s) 72
BsaHI GRCGYC 2 cut(s) 579, 753
BsaJI CCNNGG 1 cut(s) 80
Bsc4I CCNNNNNNNGG 3 cut(s) 52, 85, 790
Bse1I ACTGG 3 cut(s) 213, 399, 777
BseDI CCNNGG 1 cut(s) 80
BseLI CCNNNNNNNGG 3 cut(s) 52, 85, 790
BseMII CTCAG 3 cut(s) 483, 815, 837
BseNI ACTGG 3 cut(s) 213, 399, 777
BsePI GCGCGC 1 cut(s) 15
BseRI GAGGAG 1 cut(s) 183
BseXI GCAGC 4 cut(s) 121, 725, 763, 880
BseYI CCCAGC 1 cut(s) 897
BsgI GTGCAG 1 cut(s) 483
Bsh1236I CGCG 1 cut(s) 17
Bsh1285I CGRYCG 1 cut(s) 809
BshFI GGCC 3 cut(s) 153, 506, 944
BsiEI CGRYCG 1 cut(s) 809
BsiHKAI GWGCWC 2 cut(s) 115, 487
BsiHKCI CYCGRG 2 cut(s) 79, 108
BsiSI CCGG 1 cut(s) 72
BslI CCNNNNNNNGG 3 cut(s) 52, 85, 790
BsmAI GTCTC 1 cut(s) 398
BsmBI CGTCTC 1 cut(s) 398
BsmI GAATGC 1 cut(s) 779
BsnI GGCC 3 cut(s) 153, 506, 944
BsoBI CYCGRG 2 cut(s) 79, 108
Bsp1286I GDGCHC 2 cut(s) 115, 487
Bsp143I GATC 5 cut(s) 55, 523, 563, 869, 953
Bsp1720I GCTNAGC 1 cut(s) 801
BspACI CCGC 5 cut(s) 86, 756, 791, 794, 805
BspANI GGCC 3 cut(s) 153, 506, 944
BspCNI CTCAG 3 cut(s) 484, 814, 836
BspFNI CGCG 1 cut(s) 17
BspLI GGNNCC 2 cut(s) 69, 76
BspMAI CTGCAG 1 cut(s) 522
BspPI GGATC 4 cut(s) 63, 531, 877, 948
BsrI ACTGG 3 cut(s) 213, 399, 777
BssECI CCNNGG 1 cut(s) 80
BssHII GCGCGC 1 cut(s) 15
BssMI GATC 5 cut(s) 55, 523, 563, 869, 953
BssNI GRCGYC 2 cut(s) 579, 753
Bst4CI ACNGT 2 cut(s) 547, 697
Bst6I CTCTTC 3 cut(s) 294, 345, 609
BstACI GRCGYC 2 cut(s) 579, 753
BstC8I GCNNGC 5 cut(s) 6, 17, 220, 903, 918
BstDEI CTNAG 4 cut(s) 492, 603, 801, 823
BstFNI CGCG 1 cut(s) 17
BstHHI GCGC 2 cut(s) 17, 19
BstKTI GATC 5 cut(s) 58, 526, 566, 872, 956
BstMAI GTCTC 1 cut(s) 398
BstMBI GATC 5 cut(s) 55, 523, 563, 869, 953
BstMCI CGRYCG 1 cut(s) 809
BstMWI GCNNNNNNNGC 5 cut(s) 14, 764, 773, 797, 902
BstNSI RCATGY 1 cut(s) 8
BstSCI CCNGG 1 cut(s) 70
BstSFI CTRYAG 1 cut(s) 518
BstUI CGCG 1 cut(s) 17
BstV1I GCAGC 4 cut(s) 121, 725, 763, 880
BstX2I RGATCY 1 cut(s) 523
BstYI RGATCY 1 cut(s) 523
BsuRI GGCC 3 cut(s) 153, 506, 944
BtsI GCAGTG 1 cut(s) 515
BtsIMutI CAGTG 4 cut(s) 220, 392, 515, 693
Cac8I GCNNGC 5 cut(s) 6, 17, 220, 903, 918
CaiI CAGNNNCTG 2 cut(s) 526, 695
CfoI GCGC 2 cut(s) 17, 19
Cfr13I GGNCC 2 cut(s) 667, 943
CseI GACGC 3 cut(s) 415, 587, 761
CviAII CATG 2 cut(s) 5, 862
DdeI CTNAG 4 cut(s) 492, 603, 801, 823
DpnI GATC 5 cut(s) 57, 525, 565, 871, 955
DpnII GATC 5 cut(s) 55, 523, 563, 869, 953
EaeI YGGCCR 1 cut(s) 151
Eam1104I CTCTTC 3 cut(s) 294, 345, 609
EarI CTCTTC 3 cut(s) 294, 345, 609
EciI GGCGGA 2 cut(s) 101, 809
Eco47I GGWCC 1 cut(s) 667
Eco88I CYCGRG 2 cut(s) 79, 108
EcoT22I ATGCAT 1 cut(s) 6
Esp3I CGTCTC 1 cut(s) 398
FaeI CATG 2 cut(s) 8, 865
FaiI YATR 4 cut(s) 6, 50, 332, 863
FatI CATG 2 cut(s) 4, 861
Fnu4HI GCNGC 6 cut(s) 135, 714, 756, 777, 792, 894
Fsp4HI GCNGC 6 cut(s) 135, 714, 756, 777, 792, 894
FspBI CTAG 3 cut(s) 165, 480, 611
GlaI GCGC 2 cut(s) 16, 18
GluI GCNGC 6 cut(s) 135, 714, 756, 777, 792, 894
GsaI CCCAGC 1 cut(s) 901
HaeIII GGCC 3 cut(s) 153, 506, 944
HapII CCGG 1 cut(s) 72
HgaI GACGC 3 cut(s) 415, 587, 761
HhaI GCGC 2 cut(s) 17, 19
Hin1I GRCGYC 2 cut(s) 579, 753
Hin1II CATG 2 cut(s) 8, 865
Hin6I GCGC 2 cut(s) 15, 17
HinP1I GCGC 2 cut(s) 15, 17
HincII GTYRAC 3 cut(s) 103, 820, 835
HindII GTYRAC 3 cut(s) 103, 820, 835
HindIII AAGCTT 1 cut(s) 278
HinfI GANTC 3 cut(s) 203, 554, 595
HpaI GTTAAC 2 cut(s) 820, 835
HpaII CCGG 1 cut(s) 72
Hpy166II GTNNAC 3 cut(s) 103, 820, 835
Hpy188I TCNGA 8 cut(s) 202, 345, 384, 493, 528, 740, 826, 934
Hpy188III TCNNGA 3 cut(s) 165, 271, 627
Hpy8I GTNNAC 3 cut(s) 103, 820, 835
HpyAV CCTTC 5 cut(s) 58, 127, 635, 841, 959
HpyCH4III ACNGT 2 cut(s) 547, 697
HpyCH4IV ACGT 2 cut(s) 290, 816
HpyCH4V TGCA 7 cut(s) 4, 392, 464, 520, 713, 779, 865
HpyF10VI GCNNNNNNNGC 5 cut(s) 14, 764, 773, 797, 902
HpyF3I CTNAG 4 cut(s) 492, 603, 801, 823
HpySE526I ACGT 2 cut(s) 290, 816
Hsp92I GRCGYC 2 cut(s) 579, 753
Hsp92II CATG 2 cut(s) 8, 865
HspAI GCGC 2 cut(s) 15, 17
KspAI GTTAAC 2 cut(s) 820, 835
Kzo9I GATC 5 cut(s) 55, 523, 563, 869, 953
LmnI GCTCC 3 cut(s) 73, 80, 913
Lsp1109I GCAGC 4 cut(s) 121, 725, 763, 880
LweI GCATC 1 cut(s) 700
MaeI CTAG 3 cut(s) 165, 480, 611
MaeII ACGT 2 cut(s) 290, 816
MaeIII GTNAC 2 cut(s) 547, 697
MalI GATC 5 cut(s) 57, 525, 565, 871, 955
MboI GATC 5 cut(s) 55, 523, 563, 869, 953
MboII GAAGA 6 cut(s) 34, 171, 311, 332, 596, 626
MfeI CAATTG 1 cut(s) 873
MflI RGATCY 1 cut(s) 523
MhlI GDGCHC 2 cut(s) 115, 487
MlsI TGGCCA 1 cut(s) 153
MluCI AATT 6 cut(s) 196, 208, 452, 570, 662, 873
MluNI TGGCCA 1 cut(s) 153
MlyI GAGTC 1 cut(s) 548
MmeI TCCRAC 2 cut(s) 763, 808
MnlI CCTC 8 cut(s) 75, 82, 91, 161, 164, 610, 844, 934
Mox20I TGGCCA 1 cut(s) 153
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 178
MscI TGGCCA 1 cut(s) 153
MseI TTAA 5 cut(s) 360, 444, 573, 819, 834
Msp20I TGGCCA 1 cut(s) 153
MspA1I CMGCKG 1 cut(s) 805
MspI CCGG 1 cut(s) 72
MspR9I CCNGG 1 cut(s) 72
MunI CAATTG 1 cut(s) 873
Mva1269I GAATGC 1 cut(s) 779
MvnI CGCG 1 cut(s) 17
MwoI GCNNNNNNNGC 5 cut(s) 14, 764, 773, 797, 902
NciI CCSGG 1 cut(s) 72
NdeII GATC 5 cut(s) 55, 523, 563, 869, 953
NlaIII CATG 2 cut(s) 8, 865
NlaIV GGNNCC 2 cut(s) 69, 76
NmuCI GTSAC 1 cut(s) 697
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 8
PaeI GCATGC 1 cut(s) 8
PauI GCGCGC 1 cut(s) 15
PcsI WCGNNNNNNNCGW 1 cut(s) 958
PctI GAATGC 1 cut(s) 779
PdmI GAANNNNTTC 1 cut(s) 178
PfeI GAWTC 2 cut(s) 203, 595
PkrI GCNGC 6 cut(s) 136, 715, 757, 778, 793, 895
PleI GAGTC 1 cut(s) 548
PpsI GAGTC 1 cut(s) 548
Psp1406I AACGTT 1 cut(s) 816
PspFI CCCAGC 1 cut(s) 897
PspN4I GGNNCC 2 cut(s) 69, 76
PspPI GGNCC 2 cut(s) 667, 943
PstI CTGCAG 1 cut(s) 522
PstNI CAGNNNCTG 2 cut(s) 526, 695
PsuI RGATCY 1 cut(s) 523
PteI GCGCGC 1 cut(s) 15
SaqAI TTAA 5 cut(s) 360, 444, 573, 819, 834
SatI GCNGC 6 cut(s) 135, 714, 756, 777, 792, 894
Sau3AI GATC 5 cut(s) 55, 523, 563, 869, 953
Sau96I GGNCC 2 cut(s) 667, 943
SchI GAGTC 1 cut(s) 548
ScrFI CCNGG 1 cut(s) 72
SduI GDGCHC 2 cut(s) 115, 487
SfaNI GCATC 1 cut(s) 700
SfcI CTRYAG 1 cut(s) 518
SinI GGWCC 1 cut(s) 667
SphI GCATGC 1 cut(s) 8
Sse9I AATT 6 cut(s) 196, 208, 452, 570, 662, 873
SsiI CCGC 5 cut(s) 86, 756, 791, 794, 805
SspMI CTAG 3 cut(s) 165, 480, 611
StyD4I CCNGG 1 cut(s) 70
TaaI ACNGT 2 cut(s) 547, 697
TaiI ACGT 2 cut(s) 293, 819
TaqI TCGA 3 cut(s) 708, 845, 952
TaqII GACCGA 1 cut(s) 716
TasI AATT 6 cut(s) 196, 208, 452, 570, 662, 873
TauI GCSGC 2 cut(s) 758, 794
TfiI GAWTC 2 cut(s) 203, 595
Tru1I TTAA 5 cut(s) 360, 444, 573, 819, 834
Tru9I TTAA 5 cut(s) 360, 444, 573, 819, 834
TscAI CASTG 4 cut(s) 220, 399, 522, 700
TseFI GTSAC 1 cut(s) 697
TseI GCWGC 4 cut(s) 134, 713, 776, 893
Tsp45I GTSAC 1 cut(s) 697
TspDTI ATGAA 1 cut(s) 696
TspGWI ACGGA 1 cut(s) 35
TspRI CASTG 4 cut(s) 220, 399, 522, 700
VpaK11BI GGWCC 1 cut(s) 667
XbaI TCTAGA 1 cut(s) 164
XceI RCATGY 1 cut(s) 8
XmnI GAANNNNTTC 1 cut(s) 178
XspI CTAG 3 cut(s) 165, 480, 611
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.