pycom05g32860

WEB family protein At1g12150-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0011447
Physical Location & Seq
Forward (+)
32101821 .. 32102699
879 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g32860.1

Sequence Viewer

Length: 879 bp
ATGAAGAAAGCGCATGCGTGCGAAATGGACTCCGTGAGAGTGGTAACTATGGAGCTCAATGAAGCCACAAAGATGCTGCAGGAAGTGGCATACGAAGAGAGCTCTCTCCGAAGCTTTGTGAGTTCCCTTAGGCTGGAATTGGAAGATGTGAAGAGGGAACAGGCTGAATTGAAGGACAAAGAAATGGAAATGGAAGCTCTCACTACTAAACTAACTGATCAAATACAGAAAACCAAGGACGAGGCAGAGGCACATCGTAATGCACCTCCTAGTCCTCATGAATATGATGCACATCACAGTTCAACTGCTGAGAAGCTTTCATCTGAAACTGAAAGCACTAGGCTGGAAGCAGAAAAGATGAAGGAAAGTGCTAATCAACATAAACGAGAAGCTGAAGATAGCAGAGTTGCAGCAGAGAAAGCAGAAAAGAAATTGAAGATTGCCCTCGAAGAAGCTAAAGAAGCACGAGAAGCAGAAAAAAGAGTCCTCGGGGAGCTGAAGTTCTTATCTGGGGGAACACAGGAAGAAGGTACGTCTTCAAACTCAGAGACCGGTGCTGCCAAGATCAGACTATCGGCGGAGGAGTTTGAATCTTTGAGAAGGAAAGTCGAGGAATGTGAAAAATTGACTGAGATGAAAGAGGCAGAAAGCATAAACCAAGTGGAAGCACTCAACGTAAGAAAAAGTGAAGTAGATAAAAAGCTGGAGGCCAATTTAAAAGCCATAGAGGAAATCAAGACTGCAACAGACATGGCATTGAACAAAGCAGAGATGGCAGAGTCGGCAAAATCAGCAGTTGAGGGAGAACTTCGCAAGCGGCATCAAGAAGAACAAATGGTGCTAGGCGAGTCATCAGTATCGTTCCCCGTTCAGTTCTAG

Protein Analysis

293

Amino Acids

32.92

Weight (kDa)

5.11

Isoelectric Point (pI)

55.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WEMBL PF05701 9 - 277 5.9e-16 Weak chloroplast movement under blue light
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011447)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12150 AT1G12150
fragaria_vesca FvH4_3g00281 FvH4_3g00281
malus_domestica MD05G1362200.v1.1 MD10G1339700.v1.1
prunus_persica Prupe.4G003200_v2.0.a1
pyrus_communis pycom05g32850 pycom05g32860 pycom10g28950
rosa_chinensis RchiOBHm_Chr5g0000421
rosa_laevigata RLG00000030865
rosa_multiflora Rmu_co8243975.1_g000001
rosa_roxburghii Rroxscaffold_1G00075740
rosa_rugosa Rorug04G0383500
rosa_samantha Rh5AG003400 Rh5BG004500 Rh5CG003600 Rh5DG003500
rosa_wichuraiana Rw5G000340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 578, 817
AcuI CTGAAG 2 cut(s) 414, 518
AfaI GTAC 1 cut(s) 532
AfiI CCNNNNNNNGG 1 cut(s) 133
AgeI ACCGGT 1 cut(s) 551
AgsI TTSAA 6 cut(s) 172, 303, 436, 540, 590, 760
AloI GAACNNNNNNTCC 2 cut(s) 485, 517
AluBI AGCT 9 cut(s) 55, 102, 114, 197, 316, 392, 455, 496, 703
AluI AGCT 9 cut(s) 55, 102, 114, 197, 316, 392, 455, 496, 703
Alw21I GWGCWC 2 cut(s) 57, 104
Alw26I GTCTC 1 cut(s) 542
Ama87I CYCGRG 1 cut(s) 488
AoxI GGCC 1 cut(s) 708
ApeKI GCWGC 3 cut(s) 76, 410, 557
AsiGI ACCGGT 1 cut(s) 551
AspLEI GCGC 1 cut(s) 13
AvaI CYCGRG 1 cut(s) 488
AxyI CCTNAGG 1 cut(s) 128
BanII GRGCYC 2 cut(s) 57, 104
BauI CACGAG 1 cut(s) 465
BbsI GAAGAC 1 cut(s) 528
Bbv12I GWGCWC 2 cut(s) 57, 104
BbvI GCAGC 3 cut(s) 63, 422, 544
BccI CCATC 1 cut(s) 766
BclI TGATCA 1 cut(s) 217
BcoDI GTCTC 1 cut(s) 542
BfaI CTAG 4 cut(s) 270, 339, 842, 877
BfmI CTRYAG 1 cut(s) 77
BisI GCNGC 4 cut(s) 77, 411, 558, 818
BlsI GCNGC 4 cut(s) 78, 412, 559, 819
BmeT110I CYCGRG 1 cut(s) 488
BmsI GCATC 3 cut(s) 63, 277, 829
BpiI GAAGAC 1 cut(s) 528
BpmI CTGGAG 1 cut(s) 725
BsaBI GATNNNNATC 1 cut(s) 291
BsaI GGTCTC 1 cut(s) 542
BsaJI CCNNGG 2 cut(s) 234, 487
BsaWI WCCGGW 1 cut(s) 551
BsaXI ACNNNNNCTCC 2 cut(s) 485, 515
Bsc4I CCNNNNNNNGG 1 cut(s) 133
Bse118I RCCGGY 1 cut(s) 551
Bse21I CCTNAGG 1 cut(s) 128
Bse8I GATNNNNATC 1 cut(s) 291
BseDI CCNNGG 2 cut(s) 234, 487
BseJI GATNNNNATC 1 cut(s) 291
BseLI CCNNNNNNNGG 1 cut(s) 133
BseMII CTCAG 3 cut(s) 300, 558, 621
BseRI GAGGAG 1 cut(s) 596
BseXI GCAGC 3 cut(s) 63, 422, 544
BshFI GGCC 1 cut(s) 710
BshTI ACCGGT 1 cut(s) 551
BsiHKAI GWGCWC 2 cut(s) 57, 104
BsiHKCI CYCGRG 1 cut(s) 488
BsiSI CCGG 1 cut(s) 552
BslI CCNNNNNNNGG 1 cut(s) 133
BsmAI GTCTC 1 cut(s) 542
BsnI GGCC 1 cut(s) 710
Bso31I GGTCTC 1 cut(s) 542
BsoBI CYCGRG 1 cut(s) 488
Bsp1286I GDGCHC 2 cut(s) 57, 104
Bsp143I GATC 2 cut(s) 217, 564
BspACI CCGC 2 cut(s) 578, 817
BspANI GGCC 1 cut(s) 710
BspCNI CTCAG 3 cut(s) 301, 557, 622
BspHI TCATGA 1 cut(s) 277
BspMAI CTGCAG 1 cut(s) 81
BspTNI GGTCTC 1 cut(s) 542
BsrFI RCCGGY 1 cut(s) 551
BssAI RCCGGY 1 cut(s) 551
BssECI CCNNGG 2 cut(s) 234, 487
BssMI GATC 2 cut(s) 217, 564
BssSI CACGAG 1 cut(s) 465
BssT1I CCWWGG 1 cut(s) 234
Bst2BI CACGAG 1 cut(s) 465
Bst4CI ACNGT 1 cut(s) 299
Bst6I CTCTTC 2 cut(s) 90, 146
BstC8I GCNNGC 3 cut(s) 15, 19, 815
BstDEI CTNAG 4 cut(s) 128, 309, 544, 630
BstHHI GCGC 1 cut(s) 13
BstKTI GATC 2 cut(s) 220, 567
BstMAI GTCTC 1 cut(s) 542
BstMBI GATC 2 cut(s) 217, 564
BstMWI GCNNNNNNNGC 6 cut(s) 419, 461, 470, 773, 782, 791
BstNSI RCATGY 1 cut(s) 17
BstSFI CTRYAG 1 cut(s) 77
BstV1I GCAGC 3 cut(s) 63, 422, 544
BstV2I GAAGAC 1 cut(s) 528
Bsu36I CCTNAGG 1 cut(s) 128
BsuRI GGCC 1 cut(s) 710
Cac8I GCNNGC 3 cut(s) 15, 19, 815
CciI TCATGA 1 cut(s) 277
CfoI GCGC 1 cut(s) 13
Cfr10I RCCGGY 1 cut(s) 551
Csp6I GTAC 1 cut(s) 531
CspAI ACCGGT 1 cut(s) 551
CviAII CATG 3 cut(s) 14, 278, 751
CviQI GTAC 1 cut(s) 531
DdeI CTNAG 4 cut(s) 128, 309, 544, 630
DpnI GATC 2 cut(s) 219, 566
DpnII GATC 2 cut(s) 217, 564
DraI TTTAAA 1 cut(s) 717
Eam1104I CTCTTC 2 cut(s) 90, 146
EarI CTCTTC 2 cut(s) 90, 146
EciI GGCGGA 1 cut(s) 593
Ecl136II GAGCTC 2 cut(s) 55, 102
Eco130I CCWWGG 1 cut(s) 234
Eco24I GRGCYC 2 cut(s) 57, 104
Eco31I GGTCTC 1 cut(s) 542
Eco53kI GAGCTC 2 cut(s) 55, 102
Eco57I CTGAAG 2 cut(s) 414, 518
Eco81I CCTNAGG 1 cut(s) 128
Eco88I CYCGRG 1 cut(s) 488
EcoICRI GAGCTC 2 cut(s) 55, 102
EcoT14I CCWWGG 1 cut(s) 234
EcoT38I GRGCYC 2 cut(s) 57, 104
ErhI CCWWGG 1 cut(s) 234
FaeI CATG 3 cut(s) 17, 281, 754
FaiI YATR 9 cut(s) 15, 50, 91, 279, 285, 381, 653, 725, 752
FatI CATG 3 cut(s) 13, 277, 750
FbaI TGATCA 1 cut(s) 217
Fnu4HI GCNGC 4 cut(s) 77, 411, 558, 818
FriOI GRGCYC 2 cut(s) 57, 104
Fsp4HI GCNGC 4 cut(s) 77, 411, 558, 818
FspBI CTAG 4 cut(s) 270, 339, 842, 877
GlaI GCGC 1 cut(s) 12
GluI GCNGC 4 cut(s) 77, 411, 558, 818
GsuI CTGGAG 1 cut(s) 725
HaeIII GGCC 1 cut(s) 710
HapII CCGG 1 cut(s) 552
HhaI GCGC 1 cut(s) 13
Hin1II CATG 3 cut(s) 17, 281, 754
Hin6I GCGC 1 cut(s) 11
HinP1I GCGC 1 cut(s) 11
HindIII AAGCTT 2 cut(s) 112, 314
HinfI GANTC 5 cut(s) 29, 483, 590, 779, 848
HpaII CCGG 1 cut(s) 552
Hpy188I TCNGA 4 cut(s) 110, 325, 547, 569
Hpy188III TCNNGA 3 cut(s) 278, 736, 824
HpyAV CCTTC 4 cut(s) 166, 355, 521, 594
HpyCH4III ACNGT 1 cut(s) 299
HpyCH4IV ACGT 2 cut(s) 533, 675
HpyCH4V TGCA 5 cut(s) 79, 263, 290, 410, 743
HpyF10VI GCNNNNNNNGC 6 cut(s) 419, 461, 470, 773, 782, 791
HpyF3I CTNAG 4 cut(s) 128, 309, 544, 630
HpySE526I ACGT 2 cut(s) 533, 675
Hsp92II CATG 3 cut(s) 17, 281, 754
HspAI GCGC 1 cut(s) 11
Ksp22I TGATCA 1 cut(s) 217
Kzo9I GATC 2 cut(s) 217, 564
LmnI GCTCC 2 cut(s) 52, 493
LpnPI CCDG 8 cut(s) 65, 119, 146, 329, 495, 506, 565, 689
Lsp1109I GCAGC 3 cut(s) 63, 422, 544
LweI GCATC 3 cut(s) 63, 277, 829
MaeI CTAG 4 cut(s) 270, 339, 842, 877
MaeII ACGT 2 cut(s) 533, 675
MaeIII GTNAC 1 cut(s) 43
MalI GATC 2 cut(s) 219, 566
MboI GATC 2 cut(s) 217, 564
MhlI GDGCHC 2 cut(s) 57, 104
MluCI AATT 5 cut(s) 137, 167, 431, 623, 712
MlyI GAGTC 4 cut(s) 23, 492, 788, 857
MseI TTAA 1 cut(s) 716
MslI CAYNNNNRTG 3 cut(s) 71, 258, 282
MspI CCGG 1 cut(s) 552
MwoI GCNNNNNNNGC 6 cut(s) 419, 461, 470, 773, 782, 791
NdeII GATC 2 cut(s) 217, 564
NlaIII CATG 3 cut(s) 17, 281, 754
NspI RCATGY 1 cut(s) 17
PaeI GCATGC 1 cut(s) 17
PagI TCATGA 1 cut(s) 277
PfeI GAWTC 1 cut(s) 590
PinAI ACCGGT 1 cut(s) 551
PkrI GCNGC 4 cut(s) 78, 412, 559, 819
PleI GAGTC 4 cut(s) 23, 491, 787, 856
PpsI GAGTC 4 cut(s) 23, 491, 787, 856
Psp124BI GAGCTC 2 cut(s) 57, 104
PstI CTGCAG 1 cut(s) 81
RsaI GTAC 1 cut(s) 532
RsaNI GTAC 1 cut(s) 531
RseI CAYNNNNRTG 3 cut(s) 71, 258, 282
SacI GAGCTC 2 cut(s) 57, 104
SaqAI TTAA 1 cut(s) 716
SatI GCNGC 4 cut(s) 77, 411, 558, 818
Sau3AI GATC 2 cut(s) 217, 564
SchI GAGTC 4 cut(s) 23, 492, 788, 857
SduI GDGCHC 2 cut(s) 57, 104
SfaNI GCATC 3 cut(s) 63, 277, 829
SfcI CTRYAG 1 cut(s) 77
SmiMI CAYNNNNRTG 3 cut(s) 71, 258, 282
SphI GCATGC 1 cut(s) 17
Sse9I AATT 5 cut(s) 137, 167, 431, 623, 712
SsiI CCGC 2 cut(s) 578, 817
SspMI CTAG 4 cut(s) 270, 339, 842, 877
SstI GAGCTC 2 cut(s) 57, 104
StyI CCWWGG 1 cut(s) 234
TaaI ACNGT 1 cut(s) 299
TaiI ACGT 2 cut(s) 536, 678
TaqI TCGA 2 cut(s) 447, 609
TasI AATT 5 cut(s) 137, 167, 431, 623, 712
TauI GCSGC 1 cut(s) 820
TfiI GAWTC 1 cut(s) 590
Tru1I TTAA 1 cut(s) 716
Tru9I TTAA 1 cut(s) 716
TseI GCWGC 3 cut(s) 76, 410, 557
TspDTI ATGAA 6 cut(s) 17, 75, 294, 309, 374, 650
TspGWI ACGGA 1 cut(s) 22
XceI RCATGY 1 cut(s) 17
XspI CTAG 4 cut(s) 270, 339, 842, 877
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.