AT1G14220

Belongs to the RNase T2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0013881
Physical Location & Seq
Reverse (-)
4858450 .. 4859661
1212 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G14220.1

Sequence Viewer

Length: 687 bp
ATGGGAGCAAAGGGTTGTGTTAACGTTCTCCTCAAGCTTCTAGTGTTTCAAGGTCTTTTCGTTTCACGTCCTCAAGAAGACTTTGATTTCTTCTACTTCGTTCTTCAGTGGCCTGGAGCCTATTGCGATACATCGCGTGCTTGTTGCTATCCAACATCCGGTAAACCCGCTGCAGATTTTGGTATCCACGGTCTATGGCCTAATTATAACGGTGGTTCATGGCCATCAAATTGTGATCCCGATAGCCAATTTGATAGATCTCAGATATCGGATCTCGTAAGTAGTTTGAAAAAGAATTGGCCAACACTATCATGTCCAAGCAACGAAGGGTTCAACTTTTGGGAACACGAATGGGAGAAACACGGTACATGCTCCGAATCCGTAATGGACCAACATGAGTACTTTGAAAATGCTCTTAAACTCAAACAAAAAGCAAATCTCCTCCAAATCCTCAAAAACTCCGGAATTAATCCGGATGATGGATTCTACAATCTGGATAAGATCACCAACGCGATAAAAGATGGAATTGGATTTACGCCAGGAATAGAATGTAACAAAGATCCGGAGCGTAATGCCCAGCTTCATCAGATCTATATTTGTGTTGATACGTCAGGAACTGAGTTTATCGAATGTCCAGTTTTGCCTAGAGGAAGCTGTCCATCTCAAATCCAGTTTTCTAAGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

228

Amino Acids

25.69

Weight (kDa)

5.08

Isoelectric Point (pI)

40.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 28 - 211 1.4e-64 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013881)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14220 AT1G26820
fragaria_vesca FvH4_4g31290
malus_domestica MD13G1071500.v1.1
prunus_persica Prupe.1G275400_v2.0.a1
pyrus_communis pycom13g06410
rosa_chinensis RchiOBHm_Chr4g0439731
rosa_laevigata RLG00000006241
rosa_multiflora Rmu_sc0010226.1_g000016 Rmu_ssc0000029.1_g000011
rosa_roxburghii Rroxscaffold_5G00380670
rosa_rugosa Rorug04G0321100
rosa_samantha Rh4AG372100 Rh4BG384200 Rh4CG398900 Rh4DG378600
rosa_wichuraiana Rw4G031770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 207
AccII CGCG 2 cut(s) 136, 512
AccIII TCCGGA 3 cut(s) 461, 472, 562
AciI CCGC 1 cut(s) 168
AclI AACGTT 1 cut(s) 24
AclWI GGATC 3 cut(s) 230, 279, 554
AcoI YGGCCR 2 cut(s) 221, 299
AcuI CTGAAG 1 cut(s) 89
AfaI GTAC 2 cut(s) 367, 401
AfiI CCNNNNNNNGG 2 cut(s) 158, 479
AgsI TTSAA 4 cut(s) 50, 289, 334, 407
AjiI CACGTC 1 cut(s) 68
AjnI CCWGG 2 cut(s) 112, 538
AluBI AGCT 3 cut(s) 37, 580, 654
AluI AGCT 3 cut(s) 37, 580, 654
AlwI GGATC 3 cut(s) 230, 279, 554
AlwNI CAGNNNCTG 1 cut(s) 617
Aor13HI TCCGGA 3 cut(s) 461, 472, 562
AoxI GGCC 4 cut(s) 110, 197, 221, 299
ApeKI GCWGC 1 cut(s) 170
AseI ATTAAT 1 cut(s) 468
AspS9I GGNCC 1 cut(s) 388
AsuHPI GGTGA 1 cut(s) 496
AvaII GGWCC 1 cut(s) 388
BalI TGGCCA 2 cut(s) 223, 301
BbsI GAAGAC 1 cut(s) 84
BbvI GCAGC 1 cut(s) 157
BccI CCATC 4 cut(s) 232, 473, 515, 667
BciT130I CCWGG 2 cut(s) 114, 540
BciVI GTATCC 1 cut(s) 194
BfaI CTAG 2 cut(s) 41, 645
BfmI CTRYAG 1 cut(s) 171
BfuI GTATCC 1 cut(s) 194
BglII AGATCT 2 cut(s) 257, 588
BisI GCNGC 1 cut(s) 171
BlsI GCNGC 1 cut(s) 172
BmcAI AGTACT 1 cut(s) 401
Bme1390I CCNGG 2 cut(s) 114, 540
Bme18I GGWCC 1 cut(s) 388
BmgBI CACGTC 1 cut(s) 68
BmgT120I GGNCC 1 cut(s) 388
BmiI GGNNCC 1 cut(s) 118
BmrFI CCNGG 2 cut(s) 114, 540
BpiI GAAGAC 1 cut(s) 84
BpmI CTGGAG 1 cut(s) 135
BpuEI CTTGAG 2 cut(s) 17, 57
BsaJI CCNNGG 1 cut(s) 187
BsaWI WCCGGW 4 cut(s) 158, 461, 472, 562
Bsc4I CCNNNNNNNGG 2 cut(s) 158, 479
Bse1I ACTGG 2 cut(s) 635, 670
BseAI TCCGGA 3 cut(s) 461, 472, 562
BseBI CCWGG 2 cut(s) 114, 540
BseDI CCNNGG 1 cut(s) 187
BseGI GGATG 2 cut(s) 155, 481
BseLI CCNNNNNNNGG 2 cut(s) 158, 479
BseMII CTCAG 2 cut(s) 275, 609
BseNI ACTGG 2 cut(s) 635, 670
BseRI GAGGAG 2 cut(s) 20, 431
BseXI GCAGC 1 cut(s) 157
BseYI CCCAGC 1 cut(s) 576
Bsh1236I CGCG 2 cut(s) 136, 512
BshFI GGCC 4 cut(s) 112, 199, 223, 301
BsiSI CCGG 4 cut(s) 159, 462, 473, 563
BslI CCNNNNNNNGG 2 cut(s) 158, 479
BsnI GGCC 4 cut(s) 112, 199, 223, 301
Bsp13I TCCGGA 3 cut(s) 461, 472, 562
Bsp143I GATC 6 cut(s) 235, 257, 271, 501, 559, 588
BspACI CCGC 1 cut(s) 168
BspANI GGCC 4 cut(s) 112, 199, 223, 301
BspCNI CTCAG 2 cut(s) 274, 610
BspEI TCCGGA 3 cut(s) 461, 472, 562
BspFNI CGCG 2 cut(s) 136, 512
BspLI GGNNCC 1 cut(s) 118
BspMAI CTGCAG 1 cut(s) 175
BspPI GGATC 3 cut(s) 230, 279, 554
BsrI ACTGG 2 cut(s) 635, 670
BssECI CCNNGG 1 cut(s) 187
BssMI GATC 6 cut(s) 235, 257, 271, 501, 559, 588
Bst2UI CCWGG 2 cut(s) 114, 540
Bst4CI ACNGT 3 cut(s) 191, 212, 365
BstC8I GCNNGC 1 cut(s) 138
BstDEI CTNAG 3 cut(s) 261, 618, 678
BstDSI CCRYGG 1 cut(s) 187
BstF5I GGATG 2 cut(s) 155, 481
BstFNI CGCG 2 cut(s) 136, 512
BstKTI GATC 6 cut(s) 238, 260, 274, 504, 562, 591
BstMBI GATC 6 cut(s) 235, 257, 271, 501, 559, 588
BstNI CCWGG 2 cut(s) 114, 540
BstNSI RCATGY 1 cut(s) 372
BstSCI CCNGG 2 cut(s) 112, 538
BstSFI CTRYAG 1 cut(s) 171
BstUI CGCG 2 cut(s) 136, 512
BstV1I GCAGC 1 cut(s) 157
BstV2I GAAGAC 1 cut(s) 84
BstX2I RGATCY 4 cut(s) 257, 271, 559, 588
BstYI RGATCY 4 cut(s) 257, 271, 559, 588
BsuI GTATCC 1 cut(s) 194
BsuRI GGCC 4 cut(s) 112, 199, 223, 301
BtgI CCRYGG 1 cut(s) 187
BtgZI GCGATG 1 cut(s) 117
BtrI CACGTC 1 cut(s) 68
BtsCI GGATG 2 cut(s) 155, 481
BtsIMutI CAGTG 1 cut(s) 113
Cac8I GCNNGC 1 cut(s) 138
CaiI CAGNNNCTG 1 cut(s) 617
Cfr13I GGNCC 1 cut(s) 388
Csp6I GTAC 2 cut(s) 366, 400
CviAII CATG 4 cut(s) 219, 312, 369, 395
CviJI RGCY 9 cut(s) 37, 112, 119, 199, 223, 246, 301, 580, 654
CviKI_1 RGCY 9 cut(s) 37, 112, 119, 199, 223, 246, 301, 580, 654
CviQI GTAC 2 cut(s) 366, 400
DdeI CTNAG 3 cut(s) 261, 618, 678
DpnI GATC 6 cut(s) 237, 259, 273, 503, 561, 590
DpnII GATC 6 cut(s) 235, 257, 271, 501, 559, 588
EaeI YGGCCR 2 cut(s) 221, 299
Eco32I GATATC 1 cut(s) 267
Eco47I GGWCC 1 cut(s) 388
Eco57I CTGAAG 1 cut(s) 89
EcoRII CCWGG 2 cut(s) 112, 538
EcoRV GATATC 1 cut(s) 267
FaeI CATG 4 cut(s) 222, 315, 372, 398
FaiI YATR 7 cut(s) 196, 207, 220, 313, 370, 396, 594
FatI CATG 4 cut(s) 218, 311, 368, 394
FauI CCCGC 1 cut(s) 175
Fnu4HI GCNGC 1 cut(s) 171
FokI GGATG 2 cut(s) 142, 488
Fsp4HI GCNGC 1 cut(s) 171
FspBI CTAG 2 cut(s) 41, 645
GluI GCNGC 1 cut(s) 171
GsaI CCCAGC 1 cut(s) 580
GsuI CTGGAG 1 cut(s) 135
HaeIII GGCC 4 cut(s) 112, 199, 223, 301
HapII CCGG 4 cut(s) 159, 462, 473, 563
Hin1II CATG 4 cut(s) 222, 315, 372, 398
HincII GTYRAC 1 cut(s) 22
HindII GTYRAC 1 cut(s) 22
HindIII AAGCTT 1 cut(s) 35
HinfI GANTC 2 cut(s) 377, 483
HpaI GTTAAC 1 cut(s) 22
HpaII CCGG 4 cut(s) 159, 462, 473, 563
HphI GGTGA 1 cut(s) 496
Hpy166II GTNNAC 2 cut(s) 22, 164
Hpy188I TCNGA 4 cut(s) 264, 271, 376, 588
Hpy188III TCNNGA 7 cut(s) 74, 239, 462, 473, 494, 563, 612
Hpy8I GTNNAC 2 cut(s) 22, 164
HpyAV CCTTC 1 cut(s) 320
HpyCH4III ACNGT 3 cut(s) 191, 212, 365
HpyCH4IV ACGT 3 cut(s) 24, 67, 608
HpyCH4V TGCA 1 cut(s) 173
HpyF3I CTNAG 3 cut(s) 261, 618, 678
HpySE526I ACGT 3 cut(s) 24, 67, 608
Hsp92II CATG 4 cut(s) 222, 315, 372, 398
Kpn2I TCCGGA 3 cut(s) 461, 472, 562
KspAI GTTAAC 1 cut(s) 22
Kzo9I GATC 6 cut(s) 235, 257, 271, 501, 559, 588
LmnI GCTCC 4 cut(s) 5, 116, 377, 565
Lsp1109I GCAGC 1 cut(s) 157
MaeI CTAG 2 cut(s) 41, 645
MaeII ACGT 3 cut(s) 24, 67, 608
MaeIII GTNAC 1 cut(s) 551
MalI GATC 6 cut(s) 237, 259, 273, 503, 561, 590
MboI GATC 6 cut(s) 235, 257, 271, 501, 559, 588
MboII GAAGA 3 cut(s) 82, 89, 95
MflI RGATCY 4 cut(s) 257, 271, 559, 588
MlsI TGGCCA 2 cut(s) 223, 301
MluCI AATT 6 cut(s) 202, 229, 248, 295, 465, 525
MluNI TGGCCA 2 cut(s) 223, 301
MmeI TCCRAC 1 cut(s) 176
MnlI CCTC 5 cut(s) 41, 81, 452, 461, 641
Mox20I TGGCCA 2 cut(s) 223, 301
MroI TCCGGA 3 cut(s) 461, 472, 562
MscI TGGCCA 2 cut(s) 223, 301
MseI TTAA 3 cut(s) 21, 417, 468
MslI CAYNNNNRTG 1 cut(s) 310
Msp20I TGGCCA 2 cut(s) 223, 301
MspA1I CMGCKG 1 cut(s) 170
MspI CCGG 4 cut(s) 159, 462, 473, 563
MspR9I CCNGG 2 cut(s) 114, 540
MvaI CCWGG 2 cut(s) 114, 540
MvnI CGCG 2 cut(s) 136, 512
NdeII GATC 6 cut(s) 235, 257, 271, 501, 559, 588
NlaIII CATG 4 cut(s) 222, 315, 372, 398
NlaIV GGNNCC 1 cut(s) 118
NspI RCATGY 1 cut(s) 372
PfeI GAWTC 2 cut(s) 377, 483
PkrI GCNGC 1 cut(s) 172
PshBI ATTAAT 1 cut(s) 468
PsiI TTATAA 1 cut(s) 207
Psp1406I AACGTT 1 cut(s) 24
Psp6I CCWGG 2 cut(s) 112, 538
PspFI CCCAGC 1 cut(s) 576
PspGI CCWGG 2 cut(s) 112, 538
PspN4I GGNNCC 1 cut(s) 118
PspPI GGNCC 1 cut(s) 388
PstI CTGCAG 1 cut(s) 175
PstNI CAGNNNCTG 1 cut(s) 617
PsuI RGATCY 4 cut(s) 257, 271, 559, 588
RsaI GTAC 2 cut(s) 367, 401
RsaNI GTAC 2 cut(s) 366, 400
RseI CAYNNNNRTG 1 cut(s) 310
SaqAI TTAA 3 cut(s) 21, 417, 468
SatI GCNGC 1 cut(s) 171
Sau3AI GATC 6 cut(s) 235, 257, 271, 501, 559, 588
Sau96I GGNCC 1 cut(s) 388
ScaI AGTACT 1 cut(s) 401
ScrFI CCNGG 2 cut(s) 114, 540
SetI ASST 7 cut(s) 27, 39, 55, 70, 582, 611, 656
SfcI CTRYAG 1 cut(s) 171
SinI GGWCC 1 cut(s) 388
SmiMI CAYNNNNRTG 1 cut(s) 310
SmlI CTYRAG 2 cut(s) 32, 72
SmoI CTYRAG 2 cut(s) 32, 72
Sse9I AATT 6 cut(s) 202, 229, 248, 295, 465, 525
SsiI CCGC 1 cut(s) 168
SspMI CTAG 2 cut(s) 41, 645
StyD4I CCNGG 2 cut(s) 112, 538
TaaI ACNGT 3 cut(s) 191, 212, 365
TaiI ACGT 3 cut(s) 27, 70, 611
TaqI TCGA 1 cut(s) 627
TasI AATT 6 cut(s) 202, 229, 248, 295, 465, 525
TatI WGTACW 1 cut(s) 399
TfiI GAWTC 2 cut(s) 377, 483
Tru1I TTAA 3 cut(s) 21, 417, 468
Tru9I TTAA 3 cut(s) 21, 417, 468
TscAI CASTG 1 cut(s) 113
TseI GCWGC 1 cut(s) 170
TspDTI ATGAA 2 cut(s) 207, 572
TspGWI ACGGA 1 cut(s) 370
TspRI CASTG 1 cut(s) 113
VpaK11BI GGWCC 1 cut(s) 388
VspI ATTAAT 1 cut(s) 468
XceI RCATGY 1 cut(s) 372
XspI CTAG 2 cut(s) 41, 645
ZrmI AGTACT 1 cut(s) 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.