Rorug04G0321100

Belongs to the RNase T2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0013881
Physical Location & Seq
Forward (+)
49474011 .. 49476836
2826 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0321100.1

Sequence Viewer

Length: 771 bp
ATGGCAGCTTCTTTGTCAAATGTTGCATCGCTTCCGGGCTCTGTGAGCGTGTCTCAGATTAGAGCTTTGGCCCCTCCTCCGTGTAACTCATCCGCGGTTCCGTATTCTTCTTCCGCCAAAACGGCGCCGTTGTCGTCGTCGTCCAGCTTCTTCTCCAGGTCGCTGCGTTTGTCCCCCCTCACATTGAGCCCTAGCTCGAAGAGAAGGACTGTTAGCTCGGTCCGTGCCAGCGCTGAGGAAGCAGCATTGCAGGCGAAAGTGACCAACAAGGTGTATTTTGATATTAGCATTGGTAATCCGGTAGGGAAGCTGGCCGGAAGGATTGTGATTGGGTTGTTTGGTGATGATGTGCCCCAAACTGTGGAGAATTTCCGTTCCCTTTGCACAGGTGAGAAAGGCTTTGGGTACAAGGGTTCTGCGTTCCATCGTGTGATCAAGGATTTCATGATTCAAGGAGGGGACTTCGATAAGGGCAATGGTACTGGAGGCAAAAGCATTTATGGGCGTACATTTAAAGATGAGAACTTTAAGTTGCTTCATACTGGACCAGGAGTTCTTAGCATGGCGAATGCAGGCCCCAATACCAATGGAAGTCAGTTCTTCATATGCACAGTACAGACGCCATGGCTAAATGGGAGGCACGTTGTGTTTGGGCAAGTTTTGGAAGGCATAGAAATTGTTAGGCTGATCGAGTCGCAGGAGACAGATAGAGGTGACCGTCCTACAAAGAAGGTAGTCATTGCTGAATGCGGAGAGCTTCCAGTAGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

256

Amino Acids

27.15

Weight (kDa)

9.46

Isoelectric Point (pI)

40.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pro_isomerase PF00160 99 - 251 7.1e-51 Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013881)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14220 AT1G26820
fragaria_vesca FvH4_4g31290
malus_domestica MD13G1071500.v1.1
prunus_persica Prupe.1G275400_v2.0.a1
pyrus_communis pycom13g06410
rosa_chinensis RchiOBHm_Chr4g0439731
rosa_laevigata RLG00000006241
rosa_multiflora Rmu_sc0010226.1_g000016 Rmu_ssc0000029.1_g000011
rosa_roxburghii Rroxscaffold_5G00380670
rosa_rugosa Rorug04G0321100
rosa_samantha Rh4AG372100 Rh4BG384200 Rh4CG398900 Rh4DG378600
rosa_wichuraiana Rw4G031770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 124
AccB7I CCANNNNNTGG 1 cut(s) 361
AccII CGCG 1 cut(s) 95
AciI CCGC 4 cut(s) 93, 95, 114, 750
AcoI YGGCCR 1 cut(s) 312
AcsI RAATTY 1 cut(s) 367
AcyI GRCGYC 2 cut(s) 125, 620
AdeI CACNNNGTG 1 cut(s) 646
AfaI GTAC 4 cut(s) 407, 481, 508, 615
AfeI AGCGCT 1 cut(s) 232
AfiI CCNNNNNNNGG 1 cut(s) 361
AgsI TTSAA 1 cut(s) 452
AjnI CCWGG 2 cut(s) 155, 547
AloI GAACNNNNNNTCC 2 cut(s) 537, 569
AluBI AGCT 7 cut(s) 8, 65, 147, 195, 216, 310, 757
AluI AGCT 7 cut(s) 8, 65, 147, 195, 216, 310, 757
Alw26I GTCTC 2 cut(s) 57, 695
Aor51HI AGCGCT 1 cut(s) 232
AoxI GGCC 3 cut(s) 69, 312, 574
ApeKI GCWGC 3 cut(s) 5, 163, 242
ApoI RAATTY 1 cut(s) 367
AspLEI GCGC 2 cut(s) 127, 233
AspS9I GGNCC 4 cut(s) 70, 220, 545, 575
AsuC2I CCSGG 1 cut(s) 36
AsuHPI GGTGA 3 cut(s) 353, 401, 725
AvaII GGWCC 2 cut(s) 220, 545
BaeGI GKGCMC 1 cut(s) 354
BaeI ACNNNNGTAYC 2 cut(s) 397, 430
BanI GGYRCC 1 cut(s) 124
BanII GRGCYC 2 cut(s) 41, 191
BbvCI CCTCAGC 1 cut(s) 234
BbvI GCAGC 3 cut(s) 17, 150, 254
BccI CCATC 1 cut(s) 432
BceAI ACGGC 2 cut(s) 112, 138
BciT130I CCWGG 2 cut(s) 157, 549
BclI TGATCA 1 cut(s) 432
BcnI CCSGG 1 cut(s) 36
BcoDI GTCTC 2 cut(s) 57, 695
BfaI CTAG 1 cut(s) 192
BfoI RGCGCY 2 cut(s) 128, 234
BglI GCCNNNNNGGC 1 cut(s) 122
BisI GCNGC 3 cut(s) 6, 164, 243
BlsI GCNGC 3 cut(s) 7, 165, 244
Bme1390I CCNGG 3 cut(s) 36, 157, 549
Bme18I GGWCC 2 cut(s) 220, 545
BmgT120I GGNCC 4 cut(s) 70, 220, 545, 575
BmiI GGNNCC 4 cut(s) 72, 99, 126, 577
BmrFI CCNGG 3 cut(s) 36, 157, 549
BmsI GCATC 1 cut(s) 35
BplI GAGNNNNNCTC 2 cut(s) 37, 69
BpmI CTGGAG 2 cut(s) 139, 504
Bpu10I CCTNAGC 1 cut(s) 234
BpuMI CCSGG 1 cut(s) 36
BsaHI GRCGYC 2 cut(s) 125, 620
BsaJI CCNNGG 2 cut(s) 93, 623
BsaWI WCCGGW 1 cut(s) 298
Bsc4I CCNNNNNNNGG 1 cut(s) 361
Bse1I ACTGG 3 cut(s) 487, 547, 761
Bse3DI GCAATG 3 cut(s) 245, 481, 738
BseBI CCWGG 2 cut(s) 157, 549
BseDI CCNNGG 2 cut(s) 93, 623
BseGI GGATG 1 cut(s) 89
BseLI CCNNNNNNNGG 1 cut(s) 361
BseMI GCAATG 3 cut(s) 245, 481, 738
BseMII CTCAG 2 cut(s) 68, 225
BseNI ACTGG 3 cut(s) 487, 547, 761
BseRI GAGGAG 1 cut(s) 66
BseSI GKGCMC 1 cut(s) 354
BseXI GCAGC 3 cut(s) 17, 150, 254
Bsh1236I CGCG 1 cut(s) 95
BshFI GGCC 3 cut(s) 71, 314, 576
BshNI GGYRCC 1 cut(s) 124
BsiSI CCGG 3 cut(s) 35, 299, 315
BslFI GGGAC 2 cut(s) 157, 473
BslI CCNNNNNNNGG 1 cut(s) 361
BsmAI GTCTC 2 cut(s) 57, 695
BsmFI GGGAC 2 cut(s) 157, 473
BsmI GAATGC 2 cut(s) 574, 752
BsnI GGCC 3 cut(s) 71, 314, 576
Bsp1286I GDGCHC 3 cut(s) 41, 191, 354
Bsp143I GATC 2 cut(s) 432, 687
Bsp19I CCATGG 1 cut(s) 623
BspACI CCGC 4 cut(s) 93, 95, 114, 750
BspANI GGCC 3 cut(s) 71, 314, 576
BspCNI CTCAG 2 cut(s) 67, 226
BspFNI CGCG 1 cut(s) 95
BspHI TCATGA 1 cut(s) 444
BspLI GGNNCC 4 cut(s) 72, 99, 126, 577
BspT107I GGYRCC 1 cut(s) 124
BsrDI GCAATG 3 cut(s) 245, 481, 738
BsrI ACTGG 3 cut(s) 487, 547, 761
BssECI CCNNGG 2 cut(s) 93, 623
BssMI GATC 2 cut(s) 432, 687
BssNI GRCGYC 2 cut(s) 125, 620
BssT1I CCWWGG 1 cut(s) 623
Bst2UI CCWGG 2 cut(s) 157, 549
Bst4CI ACNGT 4 cut(s) 211, 361, 613, 719
Bst6I CTCTTC 1 cut(s) 194
BstACI GRCGYC 2 cut(s) 125, 620
BstC8I GCNNGC 4 cut(s) 229, 252, 312, 574
BstDEI CTNAG 3 cut(s) 54, 234, 557
BstDSI CCRYGG 2 cut(s) 93, 623
BstEII GGTNACC 1 cut(s) 713
BstF5I GGATG 1 cut(s) 89
BstFNI CGCG 1 cut(s) 95
BstH2I RGCGCY 2 cut(s) 128, 234
BstHHI GCGC 2 cut(s) 127, 233
BstKTI GATC 2 cut(s) 435, 690
BstMAI GTCTC 2 cut(s) 57, 695
BstMBI GATC 2 cut(s) 432, 687
BstMWI GCNNNNNNNGC 4 cut(s) 45, 122, 239, 251
BstNI CCWGG 2 cut(s) 157, 549
BstPI GGTNACC 1 cut(s) 713
BstSCI CCNGG 3 cut(s) 34, 155, 547
BstSLI GKGCMC 1 cut(s) 354
BstUI CGCG 1 cut(s) 95
BstV1I GCAGC 3 cut(s) 17, 150, 254
BsuRI GGCC 3 cut(s) 71, 314, 576
BtgI CCRYGG 2 cut(s) 93, 623
BtgZI GCGATG 1 cut(s) 12
BtsCI GGATG 1 cut(s) 89
Cac8I GCNNGC 4 cut(s) 229, 252, 312, 574
CciI TCATGA 1 cut(s) 444
CfoI GCGC 2 cut(s) 127, 233
Cfr13I GGNCC 4 cut(s) 70, 220, 545, 575
Cfr42I CCGCGG 1 cut(s) 96
CpoI CGGWCCG 1 cut(s) 220
CseI GACGC 1 cut(s) 628
Csp6I GTAC 4 cut(s) 406, 480, 507, 614
CspI CGGWCCG 1 cut(s) 220
CviAII CATG 3 cut(s) 445, 562, 624
CviQI GTAC 4 cut(s) 406, 480, 507, 614
DdeI CTNAG 3 cut(s) 54, 234, 557
DinI GGCGCC 1 cut(s) 126
DpnI GATC 2 cut(s) 434, 689
DpnII GATC 2 cut(s) 432, 687
DraI TTTAAA 1 cut(s) 514
DraIII CACNNNGTG 1 cut(s) 646
EaeI YGGCCR 1 cut(s) 312
Eam1104I CTCTTC 1 cut(s) 194
EarI CTCTTC 1 cut(s) 194
EciI GGCGGA 1 cut(s) 103
Eco130I CCWWGG 1 cut(s) 623
Eco24I GRGCYC 2 cut(s) 41, 191
Eco47I GGWCC 2 cut(s) 220, 545
Eco47III AGCGCT 1 cut(s) 232
Eco91I GGTNACC 1 cut(s) 713
EcoO109I RGGNCCY 1 cut(s) 575
EcoO65I GGTNACC 1 cut(s) 713
EcoRII CCWGG 2 cut(s) 155, 547
EcoT14I CCWWGG 1 cut(s) 623
EcoT38I GRGCYC 2 cut(s) 41, 191
EgeI GGCGCC 1 cut(s) 126
EheI GGCGCC 1 cut(s) 126
ErhI CCWWGG 1 cut(s) 623
FaeI CATG 3 cut(s) 448, 565, 627
FaiI YATR 8 cut(s) 446, 501, 540, 563, 605, 607, 625, 671
FaqI GGGAC 2 cut(s) 157, 473
FatI CATG 3 cut(s) 444, 561, 623
FauNDI CATATG 1 cut(s) 605
FbaI TGATCA 1 cut(s) 432
Fnu4HI GCNGC 3 cut(s) 6, 164, 243
FokI GGATG 1 cut(s) 76
FriOI GRGCYC 2 cut(s) 41, 191
Fsp4HI GCNGC 3 cut(s) 6, 164, 243
FspBI CTAG 1 cut(s) 192
GlaI GCGC 2 cut(s) 126, 232
GluI GCNGC 3 cut(s) 6, 164, 243
GsuI CTGGAG 2 cut(s) 139, 504
HaeII RGCGCY 2 cut(s) 128, 234
HaeIII GGCC 3 cut(s) 71, 314, 576
HapII CCGG 3 cut(s) 35, 299, 315
HgaI GACGC 1 cut(s) 628
HhaI GCGC 2 cut(s) 127, 233
Hin1I GRCGYC 2 cut(s) 125, 620
Hin1II CATG 3 cut(s) 448, 565, 627
Hin6I GCGC 2 cut(s) 125, 231
HinP1I GCGC 2 cut(s) 125, 231
HinfI GANTC 2 cut(s) 448, 692
HpaII CCGG 3 cut(s) 35, 299, 315
HphI GGTGA 3 cut(s) 353, 401, 725
Hpy188I TCNGA 1 cut(s) 57
Hpy188III TCNNGA 1 cut(s) 445
Hpy99I CGWCG 2 cut(s) 139, 142
HpyAV CCTTC 4 cut(s) 198, 312, 659, 724
HpyCH4III ACNGT 4 cut(s) 211, 361, 613, 719
HpyCH4IV ACGT 1 cut(s) 642
HpyCH4V TGCA 5 cut(s) 26, 250, 384, 572, 609
HpyF10VI GCNNNNNNNGC 4 cut(s) 45, 122, 239, 251
HpyF3I CTNAG 3 cut(s) 54, 234, 557
HpySE526I ACGT 1 cut(s) 642
Hsp92I GRCGYC 2 cut(s) 125, 620
Hsp92II CATG 3 cut(s) 448, 565, 627
HspAI GCGC 2 cut(s) 125, 231
KasI GGCGCC 1 cut(s) 124
Ksp22I TGATCA 1 cut(s) 432
KspI CCGCGG 1 cut(s) 96
Kzo9I GATC 2 cut(s) 432, 687
Lsp1109I GCAGC 3 cut(s) 17, 150, 254
LweI GCATC 1 cut(s) 35
MaeI CTAG 1 cut(s) 192
MaeII ACGT 1 cut(s) 642
MaeIII GTNAC 3 cut(s) 83, 259, 713
MalI GATC 2 cut(s) 434, 689
MboI GATC 2 cut(s) 432, 687
MboII GAAGA 5 cut(s) 99, 102, 142, 211, 592
MhlI GDGCHC 3 cut(s) 41, 191, 354
MluCI AATT 2 cut(s) 367, 675
Mly113I GGCGCC 1 cut(s) 125
MlyI GAGTC 1 cut(s) 701
MnlI CCTC 8 cut(s) 84, 87, 188, 229, 449, 479, 630, 704
MseI TTAA 2 cut(s) 513, 528
MspA1I CMGCKG 1 cut(s) 95
MspI CCGG 3 cut(s) 35, 299, 315
MspR9I CCNGG 3 cut(s) 36, 157, 549
Mva1269I GAATGC 2 cut(s) 574, 752
MvaI CCWGG 2 cut(s) 157, 549
MvnI CGCG 1 cut(s) 95
MwoI GCNNNNNNNGC 4 cut(s) 45, 122, 239, 251
NarI GGCGCC 1 cut(s) 125
NciI CCSGG 1 cut(s) 36
NcoI CCATGG 1 cut(s) 623
NdeI CATATG 1 cut(s) 605
NdeII GATC 2 cut(s) 432, 687
NlaIII CATG 3 cut(s) 448, 565, 627
NlaIV GGNNCC 4 cut(s) 72, 99, 126, 577
NmuCI GTSAC 2 cut(s) 259, 713
PagI TCATGA 1 cut(s) 444
PctI GAATGC 2 cut(s) 574, 752
PfeI GAWTC 1 cut(s) 448
PflMI CCANNNNNTGG 1 cut(s) 361
PkrI GCNGC 3 cut(s) 7, 165, 244
PleI GAGTC 1 cut(s) 700
PluTI GGCGCC 1 cut(s) 128
PpsI GAGTC 1 cut(s) 700
Psp6I CCWGG 2 cut(s) 155, 547
PspEI GGTNACC 1 cut(s) 713
PspGI CCWGG 2 cut(s) 155, 547
PspN4I GGNNCC 4 cut(s) 72, 99, 126, 577
PspPI GGNCC 4 cut(s) 70, 220, 545, 575
RsaI GTAC 4 cut(s) 407, 481, 508, 615
RsaNI GTAC 4 cut(s) 406, 480, 507, 614
Rsr2I CGGWCCG 1 cut(s) 220
RsrII CGGWCCG 1 cut(s) 220
SacII CCGCGG 1 cut(s) 96
SaqAI TTAA 2 cut(s) 513, 528
SatI GCNGC 3 cut(s) 6, 164, 243
Sau3AI GATC 2 cut(s) 432, 687
Sau96I GGNCC 4 cut(s) 70, 220, 545, 575
SchI GAGTC 1 cut(s) 701
ScrFI CCNGG 3 cut(s) 36, 157, 549
SduI GDGCHC 3 cut(s) 41, 191, 354
SfaNI GCATC 1 cut(s) 35
SfoI GGCGCC 1 cut(s) 126
Sfr303I CCGCGG 1 cut(s) 96
SgrBI CCGCGG 1 cut(s) 96
SinI GGWCC 2 cut(s) 220, 545
Sse9I AATT 2 cut(s) 367, 675
SsiI CCGC 4 cut(s) 93, 95, 114, 750
SspDI GGCGCC 1 cut(s) 124
SspMI CTAG 1 cut(s) 192
StyD4I CCNGG 3 cut(s) 34, 155, 547
StyI CCWWGG 1 cut(s) 623
TaaI ACNGT 4 cut(s) 211, 361, 613, 719
TaiI ACGT 1 cut(s) 645
TaqI TCGA 3 cut(s) 197, 465, 690
TaqII GACCGA 1 cut(s) 208
TasI AATT 2 cut(s) 367, 675
TatI WGTACW 1 cut(s) 613
TfiI GAWTC 1 cut(s) 448
Tru1I TTAA 2 cut(s) 513, 528
Tru9I TTAA 2 cut(s) 513, 528
TseFI GTSAC 2 cut(s) 259, 713
TseI GCWGC 3 cut(s) 5, 163, 242
Tsp45I GTSAC 2 cut(s) 259, 713
TspDTI ATGAA 3 cut(s) 433, 527, 592
TspGWI ACGGA 4 cut(s) 69, 90, 212, 362
Van91I CCANNNNNTGG 1 cut(s) 361
VpaK11BI GGWCC 2 cut(s) 220, 545
XapI RAATTY 1 cut(s) 367
XspI CTAG 1 cut(s) 192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.