FvH4_4g31290

Belongs to the RNase T2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0013881
Physical Location & Seq
Reverse (-)
30499355 .. 30501037
1683 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g31290.t1

Sequence Viewer

Length: 690 bp
ATGAGATACAGCACTAGTTTGATTTTGATCAAACTGCTGGTAATTCAATACCTGTCAGTCCAGGTTCTTTGCGCTTCTCAGGACTTTGATTTCTTCTACTTCGTTCAACAGTGGCCAGGAGCATACTGCGACACAAAGCACAGTTGTTGCTATCCCAAGTCAGGGAAGCCTGCTGCAGATTTTGGCATTCACGGTCTCTGGCCTAACTACAACGATGGCTCTTACCCTTCTAACTGTGATCCCGACAGTGTCTTCGACAAATCTGAGATCTCAGAGTTGATGAGCAGTCTGGAAAAGAATTGGCCGTCGATGAGCTGCCCGAGCAGTAACGGGTACAGGTTCTGGTCACATGAATGGGAGAAGCACGGCACTTGCGCCGAATCTGAACTTGACCAGAAAGATTACTTCCAAGCCGGTCTCAAGCTCAAGGAAAAGGCCAACCTTCTTCAAGCCCTTAAAAAAGCTGGTATAAAACCAGATGATGAACTATACAGCTTGGAGAGCATTGTGCAGGCTATAAAAGAGGGTGTTGGGCATACCCCAGGGATTGAGTGCAACAAAGACTCAGCTGGTAACAGCCAACTTTACCAAGTTTACTTGTGCGTTGATACTTCTGGCCAAGACATTATCGAGTGCCCAATTCTTCCTAGAGGACGATGTGCTTCCACTGTCCAATTCCCTAAATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

230

Amino Acids

25.75

Weight (kDa)

5.23

Isoelectric Point (pI)

35.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 29 - 212 4.2e-65 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013881)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14220 AT1G26820
fragaria_vesca FvH4_4g31290
malus_domestica MD13G1071500.v1.1
prunus_persica Prupe.1G275400_v2.0.a1
pyrus_communis pycom13g06410
rosa_chinensis RchiOBHm_Chr4g0439731
rosa_laevigata RLG00000006241
rosa_multiflora Rmu_sc0010226.1_g000016 Rmu_ssc0000029.1_g000011
rosa_roxburghii Rroxscaffold_5G00380670
rosa_rugosa Rorug04G0321100
rosa_samantha Rh4AG372100 Rh4BG384200 Rh4CG398900 Rh4DG378600
rosa_wichuraiana Rw4G031770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 233
AcoI YGGCCR 3 cut(s) 113, 302, 616
AcsI RAATTY 1 cut(s) 683
AfaI GTAC 1 cut(s) 335
AfiI CCNNNNNNNGG 2 cut(s) 161, 162
AgsI TTSAA 3 cut(s) 47, 107, 449
AhlI ACTAGT 1 cut(s) 14
AjnI CCWGG 3 cut(s) 60, 115, 541
AluBI AGCT 5 cut(s) 315, 424, 464, 495, 569
AluI AGCT 5 cut(s) 315, 424, 464, 495, 569
Alw26I GTCTC 2 cut(s) 200, 422
AlwI GGATC 1 cut(s) 233
AlwNI CAGNNNCTG 1 cut(s) 342
Ama87I CYCGRG 1 cut(s) 319
AoxI GGCC 5 cut(s) 113, 200, 302, 435, 616
ApeKI GCWGC 2 cut(s) 173, 315
ApoI RAATTY 1 cut(s) 683
ArsI GACNNNNNNTTYG 2 cut(s) 236, 268
AspLEI GCGC 2 cut(s) 74, 377
AvaI CYCGRG 1 cut(s) 319
BaeGI GKGCMC 1 cut(s) 638
BalI TGGCCA 2 cut(s) 115, 618
BbsI GAAGAC 1 cut(s) 244
BbvI GCAGC 2 cut(s) 160, 302
BccI CCATC 1 cut(s) 209
BceAI ACGGC 2 cut(s) 289, 382
BciT130I CCWGG 3 cut(s) 62, 117, 543
BclI TGATCA 1 cut(s) 27
BcoDI GTCTC 2 cut(s) 200, 422
BcuI ACTAGT 1 cut(s) 14
BfaI CTAG 2 cut(s) 15, 648
BfmI CTRYAG 1 cut(s) 174
BglII AGATCT 1 cut(s) 267
BisI GCNGC 2 cut(s) 174, 316
BlsI GCNGC 2 cut(s) 175, 317
Bme1390I CCNGG 3 cut(s) 62, 117, 543
BmeT110I CYCGRG 1 cut(s) 319
BmrFI CCNGG 3 cut(s) 62, 117, 543
BpiI GAAGAC 1 cut(s) 244
BpuEI CTTGAG 2 cut(s) 404, 410
BsaBI GATNNNNATC 1 cut(s) 26
BsaI GGTCTC 2 cut(s) 200, 422
BsaJI CCNNGG 2 cut(s) 541, 542
Bsc4I CCNNNNNNNGG 2 cut(s) 161, 162
Bse118I RCCGGY 1 cut(s) 413
Bse8I GATNNNNATC 1 cut(s) 26
BseBI CCWGG 3 cut(s) 62, 117, 543
BseDI CCNNGG 2 cut(s) 541, 542
BseJI GATNNNNATC 1 cut(s) 26
BseLI CCNNNNNNNGG 2 cut(s) 161, 162
BseMII CTCAG 4 cut(s) 92, 255, 285, 579
BseSI GKGCMC 1 cut(s) 638
BseXI GCAGC 2 cut(s) 160, 302
BsgI GTGCAG 1 cut(s) 530
BshFI GGCC 5 cut(s) 115, 202, 304, 437, 618
BsiHKCI CYCGRG 1 cut(s) 319
BsiSI CCGG 1 cut(s) 414
BslI CCNNNNNNNGG 2 cut(s) 161, 162
BsmAI GTCTC 2 cut(s) 200, 422
BsmI GAATGC 1 cut(s) 186
BsnI GGCC 5 cut(s) 115, 202, 304, 437, 618
Bso31I GGTCTC 2 cut(s) 200, 422
BsoBI CYCGRG 1 cut(s) 319
Bsp1286I GDGCHC 1 cut(s) 638
Bsp143I GATC 3 cut(s) 27, 238, 267
BspANI GGCC 5 cut(s) 115, 202, 304, 437, 618
BspCNI CTCAG 4 cut(s) 91, 256, 284, 578
BspMAI CTGCAG 1 cut(s) 178
BspPI GGATC 1 cut(s) 233
BspTNI GGTCTC 2 cut(s) 200, 422
BsrFI RCCGGY 1 cut(s) 413
BssAI RCCGGY 1 cut(s) 413
BssECI CCNNGG 2 cut(s) 541, 542
BssMI GATC 3 cut(s) 27, 238, 267
Bst2UI CCWGG 3 cut(s) 62, 117, 543
Bst4CI ACNGT 6 cut(s) 111, 143, 194, 236, 248, 670
BstC8I GCNNGC 2 cut(s) 171, 513
BstDEI CTNAG 4 cut(s) 78, 264, 271, 565
BstHHI GCGC 2 cut(s) 74, 377
BstKTI GATC 3 cut(s) 30, 241, 270
BstMAI GTCTC 2 cut(s) 200, 422
BstMBI GATC 3 cut(s) 27, 238, 267
BstMWI GCNNNNNNNGC 2 cut(s) 321, 501
BstNI CCWGG 3 cut(s) 62, 117, 543
BstSCI CCNGG 3 cut(s) 60, 115, 541
BstSFI CTRYAG 1 cut(s) 174
BstSLI GKGCMC 1 cut(s) 638
BstV1I GCAGC 2 cut(s) 160, 302
BstV2I GAAGAC 1 cut(s) 244
BstX2I RGATCY 1 cut(s) 267
BstYI RGATCY 1 cut(s) 267
BsuRI GGCC 5 cut(s) 115, 202, 304, 437, 618
BtsIMutI CAGTG 3 cut(s) 116, 253, 666
Cac8I GCNNGC 2 cut(s) 171, 513
CaiI CAGNNNCTG 1 cut(s) 342
CfoI GCGC 2 cut(s) 74, 377
Cfr10I RCCGGY 1 cut(s) 413
Csp6I GTAC 1 cut(s) 334
CviAII CATG 1 cut(s) 350
CviQI GTAC 1 cut(s) 334
DdeI CTNAG 4 cut(s) 78, 264, 271, 565
DpnI GATC 3 cut(s) 29, 240, 269
DpnII GATC 3 cut(s) 27, 238, 267
EaeI YGGCCR 3 cut(s) 113, 302, 616
Eco31I GGTCTC 2 cut(s) 200, 422
Eco88I CYCGRG 1 cut(s) 319
EcoRII CCWGG 3 cut(s) 60, 115, 541
FaeI CATG 1 cut(s) 353
FaiI YATR 6 cut(s) 124, 351, 470, 490, 518, 537
FatI CATG 1 cut(s) 349
FbaI TGATCA 1 cut(s) 27
Fnu4HI GCNGC 2 cut(s) 174, 316
Fsp4HI GCNGC 2 cut(s) 174, 316
FspBI CTAG 2 cut(s) 15, 648
GlaI GCGC 2 cut(s) 73, 376
GluI GCNGC 2 cut(s) 174, 316
HaeIII GGCC 5 cut(s) 115, 202, 304, 437, 618
HapII CCGG 1 cut(s) 414
HhaI GCGC 2 cut(s) 74, 377
Hin1II CATG 1 cut(s) 353
Hin6I GCGC 2 cut(s) 72, 375
HinP1I GCGC 2 cut(s) 72, 375
HinfI GANTC 2 cut(s) 380, 563
HpaII CCGG 1 cut(s) 414
Hpy166II GTNNAC 1 cut(s) 595
Hpy188I TCNGA 3 cut(s) 265, 274, 385
Hpy188III TCNNGA 3 cut(s) 80, 242, 290
Hpy8I GTNNAC 1 cut(s) 595
Hpy99I CGWCG 1 cut(s) 310
HpyAV CCTTC 2 cut(s) 237, 452
HpyCH4III ACNGT 6 cut(s) 111, 143, 194, 236, 248, 670
HpyCH4V TGCA 3 cut(s) 176, 511, 555
HpyF10VI GCNNNNNNNGC 2 cut(s) 321, 501
HpyF3I CTNAG 4 cut(s) 78, 264, 271, 565
Hsp92II CATG 1 cut(s) 353
HspAI GCGC 2 cut(s) 72, 375
Ksp22I TGATCA 1 cut(s) 27
Kzo9I GATC 3 cut(s) 27, 238, 267
LmnI GCTCC 1 cut(s) 119
Lsp1109I GCAGC 2 cut(s) 160, 302
MaeI CTAG 2 cut(s) 15, 648
MaeIII GTNAC 3 cut(s) 326, 345, 572
MalI GATC 3 cut(s) 29, 240, 269
MboI GATC 3 cut(s) 27, 238, 267
MboII GAAGA 4 cut(s) 85, 244, 437, 635
MflI RGATCY 1 cut(s) 267
MhlI GDGCHC 1 cut(s) 638
MlsI TGGCCA 2 cut(s) 115, 618
MluCI AATT 5 cut(s) 42, 298, 639, 674, 683
MluNI TGGCCA 2 cut(s) 115, 618
MlyI GAGTC 1 cut(s) 557
MnlI CCTC 2 cut(s) 517, 644
Mox20I TGGCCA 2 cut(s) 115, 618
MscI TGGCCA 2 cut(s) 115, 618
MseI TTAA 2 cut(s) 456, 688
MslI CAYNNNNRTG 1 cut(s) 352
Msp20I TGGCCA 2 cut(s) 115, 618
MspA1I CMGCKG 1 cut(s) 569
MspI CCGG 1 cut(s) 414
MspR9I CCNGG 3 cut(s) 62, 117, 543
Mva1269I GAATGC 1 cut(s) 186
MvaI CCWGG 3 cut(s) 62, 117, 543
MwoI GCNNNNNNNGC 2 cut(s) 321, 501
NdeII GATC 3 cut(s) 27, 238, 267
NlaIII CATG 1 cut(s) 353
NmuCI GTSAC 1 cut(s) 345
PasI CCCWGGG 1 cut(s) 542
PctI GAATGC 1 cut(s) 186
PfeI GAWTC 1 cut(s) 380
PflFI GACNNNGTC 1 cut(s) 248
PkrI GCNGC 2 cut(s) 175, 317
PleI GAGTC 1 cut(s) 557
PpsI GAGTC 1 cut(s) 557
Psp6I CCWGG 3 cut(s) 60, 115, 541
PspGI CCWGG 3 cut(s) 60, 115, 541
PstI CTGCAG 1 cut(s) 178
PstNI CAGNNNCTG 1 cut(s) 342
PsuI RGATCY 1 cut(s) 267
PsyI GACNNNGTC 1 cut(s) 248
PvuII CAGCTG 1 cut(s) 569
RsaI GTAC 1 cut(s) 335
RsaNI GTAC 1 cut(s) 334
RseI CAYNNNNRTG 1 cut(s) 352
SaqAI TTAA 2 cut(s) 456, 688
SatI GCNGC 2 cut(s) 174, 316
Sau3AI GATC 3 cut(s) 27, 238, 267
SchI GAGTC 1 cut(s) 557
ScrFI CCNGG 3 cut(s) 62, 117, 543
SduI GDGCHC 1 cut(s) 638
SetI ASST 9 cut(s) 54, 66, 317, 341, 426, 444, 466, 497, 571
SfcI CTRYAG 1 cut(s) 174
SmiMI CAYNNNNRTG 1 cut(s) 352
SmlI CTYRAG 2 cut(s) 419, 425
SmoI CTYRAG 2 cut(s) 419, 425
SpeI ACTAGT 1 cut(s) 14
Sse9I AATT 5 cut(s) 42, 298, 639, 674, 683
SspMI CTAG 2 cut(s) 15, 648
StyD4I CCNGG 3 cut(s) 60, 115, 541
TaaI ACNGT 6 cut(s) 111, 143, 194, 236, 248, 670
TaqI TCGA 3 cut(s) 255, 308, 630
TasI AATT 5 cut(s) 42, 298, 639, 674, 683
TfiI GAWTC 1 cut(s) 380
Tru1I TTAA 2 cut(s) 456, 688
Tru9I TTAA 2 cut(s) 456, 688
TscAI CASTG 3 cut(s) 116, 253, 673
TseFI GTSAC 1 cut(s) 345
TseI GCWGC 2 cut(s) 173, 315
Tsp45I GTSAC 1 cut(s) 345
TspDTI ATGAA 2 cut(s) 366, 498
TspRI CASTG 3 cut(s) 116, 253, 673
Tth111I GACNNNGTC 1 cut(s) 248
XapI RAATTY 1 cut(s) 683
XspI CTAG 2 cut(s) 15, 648
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.