Rroxscaffold_5G00380670

Belongs to the RNase T2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0013881
Physical Location & Seq
Reverse (-)
60843450 .. 60846763
3314 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00380670.1

Sequence Viewer

Length: 807 bp
ATGAGATACAGCACTAGTTTGATTTTGATCAAACTATTGGTAATTCAATACTTGTCAGGTCTTTGCGCTTCGCAGGACTTTGATTTCTTCTACTTCGTTCAACAGTGGCCTGGAGCATATTGCGACACAAAGCATAGTTGTTGCTATCCAAAGTCAGGGAAGCCTGCAGCAGATTTCGGCATTCACGGTCTCTGGCCAAACTACAAGGATGGCTCTTACCCTTCAAACTGTGATCCAGACAGTGTCTTCGACAAATCTGAGATATCAGAGTTGATGGGCAGTCTGGAAAAGAATTGGCCATCTATGAGCTGCCCAAGCAGTAATGGATACAGGTTCTGGTCACACGAATGGGAGAAGCATGGCACTTGCTCCGAATCTGAACTTGATCAGAAAGATTACTTCCAAGCCGGTCTCAAGCTCAAGGAAAAAGCAAACCTTCTTCAAGCCCTTAAAAAAGCTGGAATAAAACCAGACGATGAACTATACAGCTTAGAAAGCATTGTAGCAGCTATAAAAGAGGGTGTTGGGCATGCCCCAGGGATTGAGTGCAACAAGGATTCAGCTGGTAACAGCCAACTTTACCAAGTTTACTTGTGCGTTGACACTTCTGGCCAAGATATTATCGAGTGCCCAATTCTTCCTAAAGGACGATGGATTGTGATTTGTGATGCGATTGTGGTTTTTCTTTGCATTAAGGTATCGAAGACGTTTGAGCACGACAAGGAGCTTGTGAATCACCTCGTGGTGAAAGAAGCCATCGACCATGGAGAAGCTCCGGCGACAAAACAATTAGTTATGGTTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

268

Amino Acids

30.03

Weight (kDa)

5.38

Isoelectric Point (pI)

34.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 27 - 210 4.7e-64 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013881)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14220 AT1G26820
fragaria_vesca FvH4_4g31290
malus_domestica MD13G1071500.v1.1
prunus_persica Prupe.1G275400_v2.0.a1
pyrus_communis pycom13g06410
rosa_chinensis RchiOBHm_Chr4g0439731
rosa_laevigata RLG00000006241
rosa_multiflora Rmu_sc0010226.1_g000016 Rmu_ssc0000029.1_g000011
rosa_roxburghii Rroxscaffold_5G00380670
rosa_rugosa Rorug04G0321100
rosa_samantha Rh4AG372100 Rh4BG384200 Rh4CG398900 Rh4DG378600
rosa_wichuraiana Rw4G031770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 227
AcoI YGGCCR 3 cut(s) 194, 296, 610
AdeI CACNNNGTG 1 cut(s) 742
AfiI CCNNNNNNNGG 1 cut(s) 155
AgsI TTSAA 4 cut(s) 47, 101, 225, 443
AhlI ACTAGT 1 cut(s) 14
AjnI CCWGG 2 cut(s) 109, 535
AluBI AGCT 8 cut(s) 309, 418, 458, 489, 509, 563, 727, 773
AluI AGCT 8 cut(s) 309, 418, 458, 489, 509, 563, 727, 773
Alw21I GWGCWC 1 cut(s) 717
Alw26I GTCTC 2 cut(s) 194, 416
AlwI GGATC 1 cut(s) 227
AlwNI CAGNNNCTG 1 cut(s) 336
AoxI GGCC 4 cut(s) 107, 194, 296, 610
ApeKI GCWGC 3 cut(s) 167, 309, 506
ArsI GACNNNNNNTTYG 2 cut(s) 230, 262
AspLEI GCGC 1 cut(s) 68
AsuHPI GGTGA 2 cut(s) 728, 757
BaeGI GKGCMC 1 cut(s) 632
BalI TGGCCA 3 cut(s) 196, 298, 612
BauI CACGAG 1 cut(s) 740
BbsI GAAGAC 2 cut(s) 238, 710
Bbv12I GWGCWC 1 cut(s) 717
BbvI GCAGC 3 cut(s) 179, 296, 518
BccI CCATC 5 cut(s) 203, 268, 307, 645, 764
BciT130I CCWGG 2 cut(s) 111, 537
BciVI GTATCC 1 cut(s) 320
BclI TGATCA 2 cut(s) 27, 385
BcoDI GTCTC 2 cut(s) 194, 416
BcuI ACTAGT 1 cut(s) 14
BfaI CTAG 1 cut(s) 15
BfmI CTRYAG 1 cut(s) 165
BfuI GTATCC 1 cut(s) 320
BisI GCNGC 3 cut(s) 168, 310, 507
BlsI GCNGC 3 cut(s) 169, 311, 508
Bme1390I CCNGG 2 cut(s) 111, 537
BmrFI CCNGG 2 cut(s) 111, 537
BmsI GCATC 1 cut(s) 658
BpiI GAAGAC 2 cut(s) 238, 710
BpmI CTGGAG 1 cut(s) 132
BpuEI CTTGAG 2 cut(s) 398, 404
BsaBI GATNNNNATC 1 cut(s) 26
BsaI GGTCTC 2 cut(s) 194, 416
BsaJI CCNNGG 3 cut(s) 535, 536, 763
Bsc4I CCNNNNNNNGG 1 cut(s) 155
Bse118I RCCGGY 1 cut(s) 407
Bse8I GATNNNNATC 1 cut(s) 26
BseBI CCWGG 2 cut(s) 111, 537
BseDI CCNNGG 3 cut(s) 535, 536, 763
BseGI GGATG 1 cut(s) 214
BseJI GATNNNNATC 1 cut(s) 26
BseLI CCNNNNNNNGG 1 cut(s) 155
BseMII CTCAG 1 cut(s) 249
BseSI GKGCMC 1 cut(s) 632
BseXI GCAGC 3 cut(s) 179, 296, 518
BshFI GGCC 4 cut(s) 109, 196, 298, 612
BsiHKAI GWGCWC 1 cut(s) 717
BsiSI CCGG 2 cut(s) 408, 776
BslI CCNNNNNNNGG 1 cut(s) 155
BsmAI GTCTC 2 cut(s) 194, 416
BsmI GAATGC 1 cut(s) 180
BsnI GGCC 4 cut(s) 109, 196, 298, 612
Bso31I GGTCTC 2 cut(s) 194, 416
Bsp1286I GDGCHC 2 cut(s) 632, 717
Bsp143I GATC 3 cut(s) 27, 232, 385
Bsp19I CCATGG 1 cut(s) 763
BspANI GGCC 4 cut(s) 109, 196, 298, 612
BspCNI CTCAG 1 cut(s) 250
BspMAI CTGCAG 1 cut(s) 169
BspPI GGATC 1 cut(s) 227
BspTNI GGTCTC 2 cut(s) 194, 416
BsrFI RCCGGY 1 cut(s) 407
BssAI RCCGGY 1 cut(s) 407
BssECI CCNNGG 3 cut(s) 535, 536, 763
BssMI GATC 3 cut(s) 27, 232, 385
BssSI CACGAG 1 cut(s) 740
BssT1I CCWWGG 1 cut(s) 763
Bst2BI CACGAG 1 cut(s) 740
Bst2UI CCWGG 2 cut(s) 111, 537
Bst4CI ACNGT 4 cut(s) 105, 188, 230, 242
BstC8I GCNNGC 2 cut(s) 165, 531
BstDEI CTNAG 2 cut(s) 258, 490
BstDSI CCRYGG 1 cut(s) 763
BstF5I GGATG 1 cut(s) 214
BstHHI GCGC 1 cut(s) 68
BstKTI GATC 3 cut(s) 30, 235, 388
BstMAI GTCTC 2 cut(s) 194, 416
BstMBI GATC 3 cut(s) 27, 232, 385
BstMWI GCNNNNNNNGC 2 cut(s) 315, 495
BstNI CCWGG 2 cut(s) 111, 537
BstNSI RCATGY 1 cut(s) 533
BstSCI CCNGG 2 cut(s) 109, 535
BstSFI CTRYAG 1 cut(s) 165
BstSLI GKGCMC 1 cut(s) 632
BstV1I GCAGC 3 cut(s) 179, 296, 518
BstV2I GAAGAC 2 cut(s) 238, 710
BsuI GTATCC 1 cut(s) 320
BsuRI GGCC 4 cut(s) 109, 196, 298, 612
BtgI CCRYGG 1 cut(s) 763
BtsCI GGATG 1 cut(s) 214
BtsIMutI CAGTG 2 cut(s) 110, 247
Cac8I GCNNGC 2 cut(s) 165, 531
CaiI CAGNNNCTG 1 cut(s) 336
CfoI GCGC 1 cut(s) 68
Cfr10I RCCGGY 1 cut(s) 407
CviAII CATG 3 cut(s) 359, 530, 764
DdeI CTNAG 2 cut(s) 258, 490
DpnI GATC 3 cut(s) 29, 234, 387
DpnII GATC 3 cut(s) 27, 232, 385
DraIII CACNNNGTG 1 cut(s) 742
EaeI YGGCCR 3 cut(s) 194, 296, 610
Eco130I CCWWGG 1 cut(s) 763
Eco31I GGTCTC 2 cut(s) 194, 416
Eco32I GATATC 1 cut(s) 264
EcoRII CCWGG 2 cut(s) 109, 535
EcoRV GATATC 1 cut(s) 264
EcoT14I CCWWGG 1 cut(s) 763
ErhI CCWWGG 1 cut(s) 763
FaeI CATG 3 cut(s) 362, 533, 767
FaiI YATR 9 cut(s) 118, 135, 305, 360, 484, 512, 531, 765, 797
FalI AAGNNNNNCTT 2 cut(s) 420, 452
FatI CATG 3 cut(s) 358, 529, 763
FbaI TGATCA 2 cut(s) 27, 385
Fnu4HI GCNGC 3 cut(s) 168, 310, 507
FokI GGATG 1 cut(s) 221
Fsp4HI GCNGC 3 cut(s) 168, 310, 507
FspBI CTAG 1 cut(s) 15
GlaI GCGC 1 cut(s) 67
GluI GCNGC 3 cut(s) 168, 310, 507
GsuI CTGGAG 1 cut(s) 132
HaeIII GGCC 4 cut(s) 109, 196, 298, 612
HapII CCGG 2 cut(s) 408, 776
HhaI GCGC 1 cut(s) 68
Hin1II CATG 3 cut(s) 362, 533, 767
Hin6I GCGC 1 cut(s) 66
HinP1I GCGC 1 cut(s) 66
HincII GTYRAC 1 cut(s) 601
HindII GTYRAC 1 cut(s) 601
HinfI GANTC 3 cut(s) 374, 557, 733
HpaII CCGG 2 cut(s) 408, 776
HphI GGTGA 2 cut(s) 728, 757
Hpy166II GTNNAC 2 cut(s) 589, 601
Hpy188I TCNGA 5 cut(s) 259, 268, 373, 379, 390
Hpy188III TCNNGA 2 cut(s) 236, 284
Hpy8I GTNNAC 2 cut(s) 589, 601
HpyAV CCTTC 2 cut(s) 231, 446
HpyCH4III ACNGT 4 cut(s) 105, 188, 230, 242
HpyCH4IV ACGT 1 cut(s) 707
HpyCH4V TGCA 3 cut(s) 167, 549, 690
HpyF10VI GCNNNNNNNGC 2 cut(s) 315, 495
HpyF3I CTNAG 2 cut(s) 258, 490
HpySE526I ACGT 1 cut(s) 707
Hsp92II CATG 3 cut(s) 362, 533, 767
HspAI GCGC 1 cut(s) 66
Ksp22I TGATCA 2 cut(s) 27, 385
Kzo9I GATC 3 cut(s) 27, 232, 385
LmnI GCTCC 4 cut(s) 113, 374, 724, 778
Lsp1109I GCAGC 3 cut(s) 179, 296, 518
LweI GCATC 1 cut(s) 658
MaeI CTAG 1 cut(s) 15
MaeII ACGT 1 cut(s) 707
MaeIII GTNAC 2 cut(s) 339, 566
MalI GATC 3 cut(s) 29, 234, 387
MboI GATC 3 cut(s) 27, 232, 385
MboII GAAGA 5 cut(s) 79, 238, 431, 629, 715
MhlI GDGCHC 2 cut(s) 632, 717
MlsI TGGCCA 3 cut(s) 196, 298, 612
MluCI AATT 4 cut(s) 42, 292, 633, 788
MluNI TGGCCA 3 cut(s) 196, 298, 612
MnlI CCTC 2 cut(s) 511, 749
Mox20I TGGCCA 3 cut(s) 196, 298, 612
MscI TGGCCA 3 cut(s) 196, 298, 612
MseI TTAA 2 cut(s) 450, 693
MslI CAYNNNNRTG 1 cut(s) 346
Msp20I TGGCCA 3 cut(s) 196, 298, 612
MspA1I CMGCKG 1 cut(s) 563
MspI CCGG 2 cut(s) 408, 776
MspR9I CCNGG 2 cut(s) 111, 537
Mva1269I GAATGC 1 cut(s) 180
MvaI CCWGG 2 cut(s) 111, 537
MwoI GCNNNNNNNGC 2 cut(s) 315, 495
NcoI CCATGG 1 cut(s) 763
NdeII GATC 3 cut(s) 27, 232, 385
NlaIII CATG 3 cut(s) 362, 533, 767
NmuCI GTSAC 1 cut(s) 339
NspI RCATGY 1 cut(s) 533
PaeI GCATGC 1 cut(s) 533
PasI CCCWGGG 1 cut(s) 536
PctI GAATGC 1 cut(s) 180
PfeI GAWTC 3 cut(s) 374, 557, 733
PflFI GACNNNGTC 1 cut(s) 242
PkrI GCNGC 3 cut(s) 169, 311, 508
Psp6I CCWGG 2 cut(s) 109, 535
PspGI CCWGG 2 cut(s) 109, 535
PstI CTGCAG 1 cut(s) 169
PstNI CAGNNNCTG 1 cut(s) 336
PsyI GACNNNGTC 1 cut(s) 242
PvuII CAGCTG 1 cut(s) 563
RseI CAYNNNNRTG 1 cut(s) 346
SaqAI TTAA 2 cut(s) 450, 693
SatI GCNGC 3 cut(s) 168, 310, 507
Sau3AI GATC 3 cut(s) 27, 232, 385
ScrFI CCNGG 2 cut(s) 111, 537
SduI GDGCHC 2 cut(s) 632, 717
SfaNI GCATC 1 cut(s) 658
SfcI CTRYAG 1 cut(s) 165
SmiMI CAYNNNNRTG 1 cut(s) 346
SmlI CTYRAG 2 cut(s) 413, 419
SmoI CTYRAG 2 cut(s) 413, 419
SpeI ACTAGT 1 cut(s) 14
SphI GCATGC 1 cut(s) 533
Sse9I AATT 4 cut(s) 42, 292, 633, 788
SspMI CTAG 1 cut(s) 15
StyD4I CCNGG 2 cut(s) 109, 535
StyI CCWWGG 1 cut(s) 763
TaaI ACNGT 4 cut(s) 105, 188, 230, 242
TaiI ACGT 1 cut(s) 710
TaqI TCGA 4 cut(s) 249, 624, 701, 759
TasI AATT 4 cut(s) 42, 292, 633, 788
TfiI GAWTC 3 cut(s) 374, 557, 733
Tru1I TTAA 2 cut(s) 450, 693
Tru9I TTAA 2 cut(s) 450, 693
TscAI CASTG 2 cut(s) 110, 247
TseFI GTSAC 1 cut(s) 339
TseI GCWGC 3 cut(s) 167, 309, 506
Tsp45I GTSAC 1 cut(s) 339
TspDTI ATGAA 1 cut(s) 492
TspRI CASTG 2 cut(s) 110, 247
Tth111I GACNNNGTC 1 cut(s) 242
XceI RCATGY 1 cut(s) 533
XspI CTAG 1 cut(s) 15
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.