Rh4BG384200

Belongs to the RNase T2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0013881
Physical Location & Seq
Reverse (-)
53896852 .. 53898496
1645 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG384200.1

Sequence Viewer

Length: 684 bp
ATGAGATACATCACTAGTTTGATTTTGATCAAACTATTGGTAATTCAATACCTGTCAGTTCTTTGCGCTTCGCAGGACTTTGATTTCTTCTACTTCGTTCAACAGTGGCCTGGAGCATATTGCGACACAAAGCATAGTTGTTGCTATCCAAAGTCAGGGAAGCCTGCAGCAGATTTCGGCATTCACGGTCTCTGGCCAAACTACAAGGATGGCTCTTACCCTTCAAACTGTGATCCAGACAGTGTCTTCGACAAATCTGAGATATCAGAGTTGATGGGCAGTCTGGAAAAGAGTTGGCCATCTATGAGCTGCCCAAGCAGTAATGGTTACAGGTTCTGGTCACACGAATGGGAGAAGCATGGCACTTGCTCCGAATCTGAACTTGATCAGAAAGATTACTTCCAAGCCGGTCTCAAGCTCAAGGAAAAAGCAAACCTTCTTCAAGCCCTTAAAAAAACTGGAATAAAACCAGACGATGAACTATACAGCTTAGAAAGCATTGTAGCAGCTATAAAAGAGGGTGTTGGGCATGCCCCCGGGATTGAGTGCAACAAGGATTCAGCTGGTAACAGCCAACTTTACCAAGTTTACTTGTGCGTTGACACTTCTGGCCAAGATATTATCGAGTGCCCAATTCTTCCTAAAGGACGATGTGCTTCCGCGATCCAATTCCCTAAATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

227

Amino Acids

25.42

Weight (kDa)

5.38

Isoelectric Point (pI)

35.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 27 - 210 9.3e-64 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013881)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14220 AT1G26820
fragaria_vesca FvH4_4g31290
malus_domestica MD13G1071500.v1.1
prunus_persica Prupe.1G275400_v2.0.a1
pyrus_communis pycom13g06410
rosa_chinensis RchiOBHm_Chr4g0439731
rosa_laevigata RLG00000006241
rosa_multiflora Rmu_sc0010226.1_g000016 Rmu_ssc0000029.1_g000011
rosa_roxburghii Rroxscaffold_5G00380670
rosa_rugosa Rorug04G0321100
rosa_samantha Rh4AG372100 Rh4BG384200 Rh4CG398900 Rh4DG378600
rosa_wichuraiana Rw4G031770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 662
AciI CCGC 1 cut(s) 660
AclWI GGATC 2 cut(s) 227, 658
AcoI YGGCCR 3 cut(s) 194, 296, 610
AcsI RAATTY 1 cut(s) 677
AfiI CCNNNNNNNGG 1 cut(s) 155
AgsI TTSAA 4 cut(s) 47, 101, 225, 443
AhlI ACTAGT 1 cut(s) 14
AjnI CCWGG 1 cut(s) 109
AluBI AGCT 5 cut(s) 309, 418, 489, 509, 563
AluI AGCT 5 cut(s) 309, 418, 489, 509, 563
Alw26I GTCTC 2 cut(s) 194, 416
AlwI GGATC 2 cut(s) 227, 658
AlwNI CAGNNNCTG 1 cut(s) 336
Ama87I CYCGRG 1 cut(s) 536
AoxI GGCC 4 cut(s) 107, 194, 296, 610
ApeKI GCWGC 3 cut(s) 167, 309, 506
ApoI RAATTY 1 cut(s) 677
ArsI GACNNNNNNTTYG 2 cut(s) 230, 262
AspLEI GCGC 1 cut(s) 68
AsuC2I CCSGG 2 cut(s) 537, 538
AvaI CYCGRG 1 cut(s) 536
BaeGI GKGCMC 1 cut(s) 632
BalI TGGCCA 3 cut(s) 196, 298, 612
BbsI GAAGAC 1 cut(s) 238
BbvI GCAGC 3 cut(s) 179, 296, 518
BccI CCATC 3 cut(s) 203, 268, 307
BciT130I CCWGG 1 cut(s) 111
BclI TGATCA 2 cut(s) 27, 385
BcnI CCSGG 2 cut(s) 537, 538
BcoDI GTCTC 2 cut(s) 194, 416
BcuI ACTAGT 1 cut(s) 14
BfaI CTAG 1 cut(s) 15
BfmI CTRYAG 1 cut(s) 165
BisI GCNGC 3 cut(s) 168, 310, 507
BlsI GCNGC 3 cut(s) 169, 311, 508
Bme1390I CCNGG 3 cut(s) 111, 537, 538
BmeT110I CYCGRG 1 cut(s) 536
BmrFI CCNGG 3 cut(s) 111, 537, 538
BpiI GAAGAC 1 cut(s) 238
BpmI CTGGAG 1 cut(s) 132
BpuEI CTTGAG 2 cut(s) 398, 404
BpuMI CCSGG 2 cut(s) 537, 538
BsaBI GATNNNNATC 1 cut(s) 26
BsaI GGTCTC 2 cut(s) 194, 416
BsaJI CCNNGG 2 cut(s) 535, 536
Bsc4I CCNNNNNNNGG 1 cut(s) 155
Bse118I RCCGGY 1 cut(s) 407
Bse1I ACTGG 1 cut(s) 463
Bse8I GATNNNNATC 1 cut(s) 26
BseBI CCWGG 1 cut(s) 111
BseDI CCNNGG 2 cut(s) 535, 536
BseGI GGATG 1 cut(s) 214
BseJI GATNNNNATC 1 cut(s) 26
BseLI CCNNNNNNNGG 1 cut(s) 155
BseMII CTCAG 1 cut(s) 249
BseNI ACTGG 1 cut(s) 463
BseSI GKGCMC 1 cut(s) 632
BseXI GCAGC 3 cut(s) 179, 296, 518
Bsh1236I CGCG 1 cut(s) 662
BshFI GGCC 4 cut(s) 109, 196, 298, 612
BsiHKCI CYCGRG 1 cut(s) 536
BsiSI CCGG 2 cut(s) 408, 537
BslI CCNNNNNNNGG 1 cut(s) 155
BsmAI GTCTC 2 cut(s) 194, 416
BsmI GAATGC 1 cut(s) 180
BsnI GGCC 4 cut(s) 109, 196, 298, 612
Bso31I GGTCTC 2 cut(s) 194, 416
BsoBI CYCGRG 1 cut(s) 536
Bsp1286I GDGCHC 1 cut(s) 632
Bsp143I GATC 4 cut(s) 27, 232, 385, 663
BspACI CCGC 1 cut(s) 660
BspANI GGCC 4 cut(s) 109, 196, 298, 612
BspCNI CTCAG 1 cut(s) 250
BspFNI CGCG 1 cut(s) 662
BspMAI CTGCAG 1 cut(s) 169
BspPI GGATC 2 cut(s) 227, 658
BspTNI GGTCTC 2 cut(s) 194, 416
BsrFI RCCGGY 1 cut(s) 407
BsrI ACTGG 1 cut(s) 463
BssAI RCCGGY 1 cut(s) 407
BssECI CCNNGG 2 cut(s) 535, 536
BssMI GATC 4 cut(s) 27, 232, 385, 663
Bst2UI CCWGG 1 cut(s) 111
Bst4CI ACNGT 4 cut(s) 105, 188, 230, 242
BstC8I GCNNGC 2 cut(s) 165, 531
BstDEI CTNAG 2 cut(s) 258, 490
BstF5I GGATG 1 cut(s) 214
BstFNI CGCG 1 cut(s) 662
BstHHI GCGC 1 cut(s) 68
BstKTI GATC 4 cut(s) 30, 235, 388, 666
BstMAI GTCTC 2 cut(s) 194, 416
BstMBI GATC 4 cut(s) 27, 232, 385, 663
BstMWI GCNNNNNNNGC 2 cut(s) 315, 495
BstNI CCWGG 1 cut(s) 111
BstNSI RCATGY 1 cut(s) 533
BstSCI CCNGG 3 cut(s) 109, 535, 536
BstSFI CTRYAG 1 cut(s) 165
BstSLI GKGCMC 1 cut(s) 632
BstUI CGCG 1 cut(s) 662
BstV1I GCAGC 3 cut(s) 179, 296, 518
BstV2I GAAGAC 1 cut(s) 238
BsuRI GGCC 4 cut(s) 109, 196, 298, 612
BtsCI GGATG 1 cut(s) 214
BtsIMutI CAGTG 2 cut(s) 110, 247
Cac8I GCNNGC 2 cut(s) 165, 531
CaiI CAGNNNCTG 1 cut(s) 336
CfoI GCGC 1 cut(s) 68
Cfr10I RCCGGY 1 cut(s) 407
Cfr9I CCCGGG 1 cut(s) 536
CviAII CATG 2 cut(s) 359, 530
DdeI CTNAG 2 cut(s) 258, 490
DpnI GATC 4 cut(s) 29, 234, 387, 665
DpnII GATC 4 cut(s) 27, 232, 385, 663
EaeI YGGCCR 3 cut(s) 194, 296, 610
Eco31I GGTCTC 2 cut(s) 194, 416
Eco32I GATATC 1 cut(s) 264
Eco88I CYCGRG 1 cut(s) 536
EcoRII CCWGG 1 cut(s) 109
EcoRV GATATC 1 cut(s) 264
FaeI CATG 2 cut(s) 362, 533
FaiI YATR 7 cut(s) 118, 135, 305, 360, 484, 512, 531
FalI AAGNNNNNCTT 2 cut(s) 420, 452
FatI CATG 2 cut(s) 358, 529
FbaI TGATCA 2 cut(s) 27, 385
Fnu4HI GCNGC 3 cut(s) 168, 310, 507
FokI GGATG 1 cut(s) 221
Fsp4HI GCNGC 3 cut(s) 168, 310, 507
FspBI CTAG 1 cut(s) 15
GlaI GCGC 1 cut(s) 67
GluI GCNGC 3 cut(s) 168, 310, 507
GsuI CTGGAG 1 cut(s) 132
HaeIII GGCC 4 cut(s) 109, 196, 298, 612
HapII CCGG 2 cut(s) 408, 537
HhaI GCGC 1 cut(s) 68
Hin1II CATG 2 cut(s) 362, 533
Hin6I GCGC 1 cut(s) 66
HinP1I GCGC 1 cut(s) 66
HincII GTYRAC 1 cut(s) 601
HindII GTYRAC 1 cut(s) 601
HinfI GANTC 2 cut(s) 374, 557
HpaII CCGG 2 cut(s) 408, 537
Hpy166II GTNNAC 2 cut(s) 589, 601
Hpy188I TCNGA 5 cut(s) 259, 268, 373, 379, 390
Hpy188III TCNNGA 2 cut(s) 236, 284
Hpy8I GTNNAC 2 cut(s) 589, 601
HpyAV CCTTC 2 cut(s) 231, 446
HpyCH4III ACNGT 4 cut(s) 105, 188, 230, 242
HpyCH4V TGCA 2 cut(s) 167, 549
HpyF10VI GCNNNNNNNGC 2 cut(s) 315, 495
HpyF3I CTNAG 2 cut(s) 258, 490
Hsp92II CATG 2 cut(s) 362, 533
HspAI GCGC 1 cut(s) 66
Ksp22I TGATCA 2 cut(s) 27, 385
Kzo9I GATC 4 cut(s) 27, 232, 385, 663
LmnI GCTCC 2 cut(s) 113, 374
Lsp1109I GCAGC 3 cut(s) 179, 296, 518
MaeI CTAG 1 cut(s) 15
MaeIII GTNAC 3 cut(s) 326, 339, 566
MalI GATC 4 cut(s) 29, 234, 387, 665
MboI GATC 4 cut(s) 27, 232, 385, 663
MboII GAAGA 4 cut(s) 79, 238, 431, 629
MhlI GDGCHC 1 cut(s) 632
MlsI TGGCCA 3 cut(s) 196, 298, 612
MluCI AATT 4 cut(s) 42, 633, 668, 677
MluNI TGGCCA 3 cut(s) 196, 298, 612
MnlI CCTC 1 cut(s) 511
Mox20I TGGCCA 3 cut(s) 196, 298, 612
MscI TGGCCA 3 cut(s) 196, 298, 612
MseI TTAA 2 cut(s) 450, 682
MslI CAYNNNNRTG 1 cut(s) 346
Msp20I TGGCCA 3 cut(s) 196, 298, 612
MspA1I CMGCKG 1 cut(s) 563
MspI CCGG 2 cut(s) 408, 537
MspR9I CCNGG 3 cut(s) 111, 537, 538
Mva1269I GAATGC 1 cut(s) 180
MvaI CCWGG 1 cut(s) 111
MvnI CGCG 1 cut(s) 662
MwoI GCNNNNNNNGC 2 cut(s) 315, 495
NciI CCSGG 2 cut(s) 537, 538
NdeII GATC 4 cut(s) 27, 232, 385, 663
NlaIII CATG 2 cut(s) 362, 533
NmuCI GTSAC 1 cut(s) 339
NspI RCATGY 1 cut(s) 533
PaeI GCATGC 1 cut(s) 533
PctI GAATGC 1 cut(s) 180
PfeI GAWTC 2 cut(s) 374, 557
PflFI GACNNNGTC 1 cut(s) 242
PkrI GCNGC 3 cut(s) 169, 311, 508
Psp6I CCWGG 1 cut(s) 109
PspGI CCWGG 1 cut(s) 109
PstI CTGCAG 1 cut(s) 169
PstNI CAGNNNCTG 1 cut(s) 336
PsyI GACNNNGTC 1 cut(s) 242
PvuII CAGCTG 1 cut(s) 563
RseI CAYNNNNRTG 1 cut(s) 346
SaqAI TTAA 2 cut(s) 450, 682
SatI GCNGC 3 cut(s) 168, 310, 507
Sau3AI GATC 4 cut(s) 27, 232, 385, 663
ScrFI CCNGG 3 cut(s) 111, 537, 538
SduI GDGCHC 1 cut(s) 632
SetI ASST 8 cut(s) 54, 311, 335, 420, 438, 491, 511, 565
SfcI CTRYAG 1 cut(s) 165
SmaI CCCGGG 1 cut(s) 538
SmiMI CAYNNNNRTG 1 cut(s) 346
SmlI CTYRAG 2 cut(s) 413, 419
SmoI CTYRAG 2 cut(s) 413, 419
SpeI ACTAGT 1 cut(s) 14
SphI GCATGC 1 cut(s) 533
Sse9I AATT 4 cut(s) 42, 633, 668, 677
SsiI CCGC 1 cut(s) 660
SspMI CTAG 1 cut(s) 15
StyD4I CCNGG 3 cut(s) 109, 535, 536
TaaI ACNGT 4 cut(s) 105, 188, 230, 242
TaqI TCGA 2 cut(s) 249, 624
TasI AATT 4 cut(s) 42, 633, 668, 677
TfiI GAWTC 2 cut(s) 374, 557
Tru1I TTAA 2 cut(s) 450, 682
Tru9I TTAA 2 cut(s) 450, 682
TscAI CASTG 2 cut(s) 110, 247
TseFI GTSAC 1 cut(s) 339
TseI GCWGC 3 cut(s) 167, 309, 506
Tsp45I GTSAC 1 cut(s) 339
TspDTI ATGAA 1 cut(s) 492
TspMI CCCGGG 1 cut(s) 536
TspRI CASTG 2 cut(s) 110, 247
Tth111I GACNNNGTC 1 cut(s) 242
XapI RAATTY 1 cut(s) 677
XceI RCATGY 1 cut(s) 533
XmaI CCCGGG 1 cut(s) 536
XspI CTAG 1 cut(s) 15
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.