Rmu_sc0010226.1_g000016

Belongs to the RNase T2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0010226.1
OG0013881
Physical Location & Seq
Forward (+)
64927 .. 68076
3150 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0010226.1_g000016.1.cds

Sequence Viewer

Length: 759 bp
atgagatacagcactagtttgattttgatcaaactattggtaattcaatacctgtcagttctttgcgcttcgcaggactttgatttcttctacttcgttcaacagtggcctggagcatattgcgacacaaagcatagttgttgctatccaaagtcagggaagcctgcagcagatttcggcattcacggtctctggccaaactacaaggatggctcttacccttcaaactgtgatccagacagtgtcttcgacaaatctgagatctcagagttgatgggcagtctggaaaagagttggccatctatgagctgcccaagcagtaatggttacaggttctggtcacacgaatgggagaagcatggcacttgctccgaatctgaacttgatcagaaagattacttccaagccggtctcaagctcaaggaaaaagcaaaccttcttcatgcccttaaaaaagctggaataaaaccagacgatgaactatacagcttagaaagcattgtagcagctataaaagagggtgttgggcatgccccagggattgagtgcaacaaggattcagctggtaacaaccaactttaccaagtttacttgcggctacggcggctcggggcttgcaggagggaggccggaggcgtccttgcccgttctgggtacagttcgaggtcgggcttgctggtttctaggaagccacctcacctgcaactgcaggttctgtgcagcactgcaaaacccgtcgccggcgactccacccaatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

252

Amino Acids

28.02

Weight (kDa)

7.98

Isoelectric Point (pI)

43.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013881)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14220 AT1G26820
fragaria_vesca FvH4_4g31290
malus_domestica MD13G1071500.v1.1
prunus_persica Prupe.1G275400_v2.0.a1
pyrus_communis pycom13g06410
rosa_chinensis RchiOBHm_Chr4g0439731
rosa_laevigata RLG00000006241
rosa_multiflora Rmu_sc0010226.1_g000016 Rmu_ssc0000029.1_g000011
rosa_roxburghii Rroxscaffold_5G00380670
rosa_rugosa Rorug04G0321100
rosa_samantha Rh4AG372100 Rh4BG384200 Rh4CG398900 Rh4DG378600
rosa_wichuraiana Rw4G031770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 708
Acc36I ACCTGC 2 cut(s) 700, 708
AciI CCGC 2 cut(s) 595, 604
AclWI GGATC 1 cut(s) 227
AcoI YGGCCR 2 cut(s) 194, 296
AcyI GRCGYC 1 cut(s) 636
AfaI GTAC 1 cut(s) 656
AfiI CCNNNNNNNGG 3 cut(s) 155, 651, 740
AgsI TTSAA 3 cut(s) 47, 101, 225
AhlI ACTAGT 1 cut(s) 14
AjnI CCWGG 2 cut(s) 109, 535
AluBI AGCT 6 cut(s) 309, 418, 458, 489, 509, 563
AluI AGCT 6 cut(s) 309, 418, 458, 489, 509, 563
Alw26I GTCTC 2 cut(s) 194, 416
AlwI GGATC 1 cut(s) 227
AlwNI CAGNNNCTG 2 cut(s) 336, 715
Ama87I CYCGRG 1 cut(s) 608
AoxI GGCC 4 cut(s) 107, 194, 296, 627
ApeKI GCWGC 4 cut(s) 167, 309, 506, 720
ArsI GACNNNNNNTTYG 2 cut(s) 230, 262
AspLEI GCGC 1 cut(s) 68
AsuHPI GGTGA 1 cut(s) 689
AvaI CYCGRG 1 cut(s) 608
BalI TGGCCA 2 cut(s) 196, 298
BbsI GAAGAC 1 cut(s) 238
BbvI GCAGC 4 cut(s) 179, 296, 518, 732
BccI CCATC 3 cut(s) 203, 268, 307
BceAI ACGGC 1 cut(s) 617
BciT130I CCWGG 2 cut(s) 111, 537
BclI TGATCA 2 cut(s) 27, 385
BcoDI GTCTC 2 cut(s) 194, 416
BcuI ACTAGT 1 cut(s) 14
BfaI CTAG 2 cut(s) 15, 684
BfmI CTRYAG 2 cut(s) 165, 707
BfuAI ACCTGC 2 cut(s) 700, 708
BglII AGATCT 1 cut(s) 261
BisI GCNGC 6 cut(s) 168, 310, 507, 596, 605, 721
BlsI GCNGC 6 cut(s) 169, 311, 508, 597, 606, 722
Bme1390I CCNGG 2 cut(s) 111, 537
BmeT110I CYCGRG 1 cut(s) 608
BmrFI CCNGG 2 cut(s) 111, 537
BpiI GAAGAC 1 cut(s) 238
BpmI CTGGAG 1 cut(s) 132
BpuEI CTTGAG 2 cut(s) 398, 404
BsaBI GATNNNNATC 1 cut(s) 26
BsaHI GRCGYC 1 cut(s) 636
BsaI GGTCTC 2 cut(s) 194, 416
BsaJI CCNNGG 2 cut(s) 535, 536
Bsc4I CCNNNNNNNGG 3 cut(s) 155, 651, 740
Bse118I RCCGGY 2 cut(s) 407, 740
Bse8I GATNNNNATC 1 cut(s) 26
BseBI CCWGG 2 cut(s) 111, 537
BseDI CCNNGG 2 cut(s) 535, 536
BseGI GGATG 1 cut(s) 214
BseJI GATNNNNATC 1 cut(s) 26
BseLI CCNNNNNNNGG 3 cut(s) 155, 651, 740
BseMII CTCAG 2 cut(s) 249, 279
BseXI GCAGC 4 cut(s) 179, 296, 518, 732
BsgI GTGCAG 1 cut(s) 739
BshFI GGCC 4 cut(s) 109, 196, 298, 629
BsiHKCI CYCGRG 1 cut(s) 608
BsiSI CCGG 3 cut(s) 408, 630, 741
BslI CCNNNNNNNGG 3 cut(s) 155, 651, 740
BsmAI GTCTC 2 cut(s) 194, 416
BsmI GAATGC 1 cut(s) 180
BsnI GGCC 4 cut(s) 109, 196, 298, 629
Bso31I GGTCTC 2 cut(s) 194, 416
BsoBI CYCGRG 1 cut(s) 608
Bsp143I GATC 4 cut(s) 27, 232, 261, 385
BspACI CCGC 2 cut(s) 595, 604
BspANI GGCC 4 cut(s) 109, 196, 298, 629
BspCNI CTCAG 2 cut(s) 250, 278
BspMAI CTGCAG 2 cut(s) 169, 711
BspMI ACCTGC 2 cut(s) 700, 708
BspPI GGATC 1 cut(s) 227
BspTNI GGTCTC 2 cut(s) 194, 416
BsrFI RCCGGY 2 cut(s) 407, 740
BssAI RCCGGY 2 cut(s) 407, 740
BssECI CCNNGG 2 cut(s) 535, 536
BssMI GATC 4 cut(s) 27, 232, 261, 385
BssNI GRCGYC 1 cut(s) 636
Bst2UI CCWGG 2 cut(s) 111, 537
Bst4CI ACNGT 5 cut(s) 105, 188, 230, 242, 659
BstACI GRCGYC 1 cut(s) 636
BstC8I GCNNGC 5 cut(s) 165, 531, 616, 674, 742
BstDEI CTNAG 3 cut(s) 258, 265, 490
BstF5I GGATG 1 cut(s) 214
BstHHI GCGC 1 cut(s) 68
BstKTI GATC 4 cut(s) 30, 235, 264, 388
BstMAI GTCTC 2 cut(s) 194, 416
BstMBI GATC 4 cut(s) 27, 232, 261, 385
BstMWI GCNNNNNNNGC 4 cut(s) 315, 495, 601, 604
BstNI CCWGG 2 cut(s) 111, 537
BstNSI RCATGY 1 cut(s) 533
BstSCI CCNGG 2 cut(s) 109, 535
BstSFI CTRYAG 2 cut(s) 165, 707
BstV1I GCAGC 4 cut(s) 179, 296, 518, 732
BstV2I GAAGAC 1 cut(s) 238
BstX2I RGATCY 1 cut(s) 261
BstYI RGATCY 1 cut(s) 261
BsuRI GGCC 4 cut(s) 109, 196, 298, 629
BtsCI GGATG 1 cut(s) 214
BtsI GCAGTG 1 cut(s) 723
BtsIMutI CAGTG 3 cut(s) 110, 247, 723
BveI ACCTGC 2 cut(s) 700, 708
Cac8I GCNNGC 5 cut(s) 165, 531, 616, 674, 742
CaiI CAGNNNCTG 2 cut(s) 336, 715
CfoI GCGC 1 cut(s) 68
Cfr10I RCCGGY 2 cut(s) 407, 740
CseI GACGC 1 cut(s) 625
Csp6I GTAC 1 cut(s) 655
CviAII CATG 3 cut(s) 359, 443, 530
CviQI GTAC 1 cut(s) 655
DdeI CTNAG 3 cut(s) 258, 265, 490
DpnI GATC 4 cut(s) 29, 234, 263, 387
DpnII GATC 4 cut(s) 27, 232, 261, 385
EaeI YGGCCR 2 cut(s) 194, 296
Eco31I GGTCTC 2 cut(s) 194, 416
Eco88I CYCGRG 1 cut(s) 608
EcoRII CCWGG 2 cut(s) 109, 535
FaeI CATG 3 cut(s) 362, 446, 533
FaiI YATR 8 cut(s) 118, 135, 305, 360, 444, 484, 512, 531
FalI AAGNNNNNCTT 2 cut(s) 420, 452
FatI CATG 3 cut(s) 358, 442, 529
FbaI TGATCA 2 cut(s) 27, 385
Fnu4HI GCNGC 6 cut(s) 168, 310, 507, 596, 605, 721
FokI GGATG 1 cut(s) 221
Fsp4HI GCNGC 6 cut(s) 168, 310, 507, 596, 605, 721
FspBI CTAG 2 cut(s) 15, 684
GlaI GCGC 1 cut(s) 67
GluI GCNGC 6 cut(s) 168, 310, 507, 596, 605, 721
GsuI CTGGAG 1 cut(s) 132
HaeIII GGCC 4 cut(s) 109, 196, 298, 629
HapII CCGG 3 cut(s) 408, 630, 741
HgaI GACGC 1 cut(s) 625
HhaI GCGC 1 cut(s) 68
Hin1I GRCGYC 1 cut(s) 636
Hin1II CATG 3 cut(s) 362, 446, 533
Hin6I GCGC 1 cut(s) 66
HinP1I GCGC 1 cut(s) 66
HinfI GANTC 3 cut(s) 374, 557, 746
HpaII CCGG 3 cut(s) 408, 630, 741
HphI GGTGA 1 cut(s) 689
Hpy166II GTNNAC 1 cut(s) 589
Hpy188I TCNGA 5 cut(s) 259, 268, 373, 379, 390
Hpy188III TCNNGA 2 cut(s) 236, 284
Hpy8I GTNNAC 1 cut(s) 589
Hpy99I CGWCG 1 cut(s) 740
HpyAV CCTTC 2 cut(s) 231, 446
HpyCH4III ACNGT 5 cut(s) 105, 188, 230, 242, 659
HpyCH4V TGCA 7 cut(s) 167, 549, 618, 703, 709, 720, 728
HpyF10VI GCNNNNNNNGC 4 cut(s) 315, 495, 601, 604
HpyF3I CTNAG 3 cut(s) 258, 265, 490
Hsp92I GRCGYC 1 cut(s) 636
Hsp92II CATG 3 cut(s) 362, 446, 533
HspAI GCGC 1 cut(s) 66
KroI GCCGGC 1 cut(s) 740
KroNI GCCGGC 1 cut(s) 742
Ksp22I TGATCA 2 cut(s) 27, 385
Kzo9I GATC 4 cut(s) 27, 232, 261, 385
LmnI GCTCC 2 cut(s) 113, 374
Lsp1109I GCAGC 4 cut(s) 179, 296, 518, 732
MaeI CTAG 2 cut(s) 15, 684
MaeIII GTNAC 3 cut(s) 326, 339, 566
MalI GATC 4 cut(s) 29, 234, 263, 387
MboI GATC 4 cut(s) 27, 232, 261, 385
MboII GAAGA 3 cut(s) 79, 238, 431
MflI RGATCY 1 cut(s) 261
MlsI TGGCCA 2 cut(s) 196, 298
MluCI AATT 1 cut(s) 42
MluNI TGGCCA 2 cut(s) 196, 298
MlyI GAGTC 1 cut(s) 740
MnlI CCTC 6 cut(s) 511, 615, 619, 626, 657, 705
Mox20I TGGCCA 2 cut(s) 196, 298
MreI CGCCGGCG 1 cut(s) 740
MroNI GCCGGC 1 cut(s) 740
MscI TGGCCA 2 cut(s) 196, 298
MseI TTAA 1 cut(s) 450
MslI CAYNNNNRTG 1 cut(s) 346
Msp20I TGGCCA 2 cut(s) 196, 298
MspA1I CMGCKG 1 cut(s) 563
MspI CCGG 3 cut(s) 408, 630, 741
MspR9I CCNGG 2 cut(s) 111, 537
Mva1269I GAATGC 1 cut(s) 180
MvaI CCWGG 2 cut(s) 111, 537
MwoI GCNNNNNNNGC 4 cut(s) 315, 495, 601, 604
NaeI GCCGGC 1 cut(s) 742
NdeII GATC 4 cut(s) 27, 232, 261, 385
NgoMIV GCCGGC 1 cut(s) 740
NlaIII CATG 3 cut(s) 362, 446, 533
NmuCI GTSAC 1 cut(s) 339
NspI RCATGY 1 cut(s) 533
PaeI GCATGC 1 cut(s) 533
PaqCI CACCTGC 1 cut(s) 708
PasI CCCWGGG 1 cut(s) 536
PctI GAATGC 1 cut(s) 180
PdiI GCCGGC 1 cut(s) 742
PfeI GAWTC 2 cut(s) 374, 557
PflFI GACNNNGTC 1 cut(s) 242
PkrI GCNGC 6 cut(s) 169, 311, 508, 597, 606, 722
PleI GAGTC 1 cut(s) 740
PpsI GAGTC 1 cut(s) 740
Psp6I CCWGG 2 cut(s) 109, 535
PspGI CCWGG 2 cut(s) 109, 535
PstI CTGCAG 2 cut(s) 169, 711
PstNI CAGNNNCTG 2 cut(s) 336, 715
PsuI RGATCY 1 cut(s) 261
PsyI GACNNNGTC 1 cut(s) 242
PvuII CAGCTG 1 cut(s) 563
RsaI GTAC 1 cut(s) 656
RsaNI GTAC 1 cut(s) 655
RseI CAYNNNNRTG 1 cut(s) 346
SaqAI TTAA 1 cut(s) 450
SatI GCNGC 6 cut(s) 168, 310, 507, 596, 605, 721
Sau3AI GATC 4 cut(s) 27, 232, 261, 385
SchI GAGTC 1 cut(s) 740
ScrFI CCNGG 2 cut(s) 111, 537
SfcI CTRYAG 2 cut(s) 165, 707
SgrAI CRCCGGYG 1 cut(s) 740
SmiMI CAYNNNNRTG 1 cut(s) 346
SmlI CTYRAG 2 cut(s) 413, 419
SmoI CTYRAG 2 cut(s) 413, 419
SpeI ACTAGT 1 cut(s) 14
SphI GCATGC 1 cut(s) 533
Sse9I AATT 1 cut(s) 42
SsiI CCGC 2 cut(s) 595, 604
SspMI CTAG 2 cut(s) 15, 684
StyD4I CCNGG 2 cut(s) 109, 535
TaaI ACNGT 5 cut(s) 105, 188, 230, 242, 659
TaqI TCGA 2 cut(s) 249, 662
TasI AATT 1 cut(s) 42
TauI GCSGC 2 cut(s) 598, 607
TfiI GAWTC 2 cut(s) 374, 557
Tru1I TTAA 1 cut(s) 450
Tru9I TTAA 1 cut(s) 450
TscAI CASTG 3 cut(s) 110, 247, 730
TseFI GTSAC 1 cut(s) 339
TseI GCWGC 4 cut(s) 167, 309, 506, 720
Tsp45I GTSAC 1 cut(s) 339
TspDTI ATGAA 2 cut(s) 431, 492
TspRI CASTG 3 cut(s) 110, 247, 730
Tth111I GACNNNGTC 1 cut(s) 242
XceI RCATGY 1 cut(s) 533
XspI CTAG 2 cut(s) 15, 684
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.