Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0007227

Physical Location & Seq

Genomic Coordinates Reverse (-)

5495373 .. 5497723

Gene Structure 2351 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G16010.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1329 bp

ATGTCTGAGCTAAAAGAGCGCCTGCTTCCTCCAAGACCTGCTTCAGCTATGAACCTCAGAGATGCTTCTGTGACTCGGCCTTCTGCTTCTGGAAGACCACCCCTTCTTGGAGTTGATGTCTTGGGTCTGAAGAAGCGTGGACAAGGCCTACGCTCTTGGATTCGTGTTGATACTTCTGGTAATACTCAGGTCATGGAGGTTGACAAGTTCACTATGATGCGTAGGTGTGACTTACCCGCCCGTGATTTAAGGCTGCTTGATCCCTTATTCGTGTATCCCTCTACTATCCTAGGCCGAGAGAAGGCTATTGTTGTCAACTTAGAGCAGATCCGTTGTATTATTACTGCAGACGAAGTTCTGCTTCTGAATTCCCTTGACAACTACGTGCTGCGCTATGTTGTTGAGTTGCAGCAGAGACTAAAGACTAGTAGTGTAGGTGAGATGTGGCAGCAGGAAAATTCCCAGTTGAGTAGGAGGAGGAGCAGGAGTTTTGACAATGCTTTTGAGAATTCATCCCCTGATTATTTACCTTTCGAGTTTAGAGCTCTTGAAATAGCCCTTGAAGCTGCTTGTACGTTTCTCGATTCCCAGGCTTCAGAGTTGGAGATTGAGGCATACCCGTTATTAGATGAGCTTACCTCAAAGATCAGTACACTAAACTTGGAGCGTGTGCGTAGACTAAAGAGCAGGCTTGTAGCACTGACAAGAAGAGTTCAGAAGGTTCGGGATGAGATCGAGCAGCTAATGGATGATGATGGAGATATGGCAGAAATGTATCTAACAGAGAAGAAGAGGAGAATGGAAGGATCTATGTACGGTGACCAGTCCTTATTGGGTTACAGGTCAAATGATGGTCTATCTGTTTCTGCACCGGTGTCTCCTGTTTCTTCTCCACCTGATTCCCGAAGACTTGATAAAAGCTTGAGCATTGCGAGGAGTCGGCATGACAGTGCAAGGAGTTCTGAAGGTGCAGAGAATATAGAAGAGCTAGAGATGTTGCTGGAAGCTTACTTTGTCGTCATTGATAGTACTCTAAACAAGTTGACATCGTTAAAGGAGTACATTGATGACACTGAAGATTTCATTAACATTCAACTGGATAATGTGCGGAATCAGCTGATCCAGTTCGAGTTGCTTCTGACGACTGCAACGTTTGTTGTAGCCATATTTGGGGTCGTTGCTGGGATCTTTGGGATGAACTTTGAGATAGACTTCTTCAATCAGCCAGGTGCATTCAGATGGGTACTCATAATTACTGGAGTATGTGGCTTTGTGATATTTTCGGCATTCGTCTGGTTCTTCAAGTACAGAAGACTCATGCCTCTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0008324 GO:0015075 GO:0015095 GO:0015318 GO:0015693 GO:0022857 GO:0022890 GO:0030001 GO:0034220 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0070838 GO:0072511 GO:0098655 GO:0098660 GO:0098662 GO:1903830

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CorA

Protein Analysis

442

Amino Acids

50.42

Weight (kDa)

5.26

Isoelectric Point (pI)

60.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MRS2-like	PF22099	57 - 266	1.2e-26	Magnesium transporter MRS2-like
MRS2-like	PF22099	322 - 370	3.6e-07	Magnesium transporter MRS2-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007227)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G16010 AT1G16010 AT1G16010 AT1G80900 AT1G80900
fragaria_vesca	FvH4_6g28490
malus_domestica	MD09G1225700.v1.1 MD17G1224500.v1.1
prunus_persica	Prupe.3G100200_v2.0.a1 Prupe.3G100200_v2.0.a1 Prupe.3G100200_v2.0.a1 Prupe.3G100200_v2.0.a1 Prupe.3G100200_v2.0.a1
pyrus_communis	pycom09g14520 pycom17g22820
rosa_chinensis	RchiOBHm_Chr2g0132741
rosa_laevigata	RLG00000019274
rosa_multiflora	Rmu_sc0004972.1_g000001 Rmu_ssc0000074.1_g000006
rosa_roxburghii	Rroxscaffold_2G00111690
rosa_rugosa	Rorug02G0307000 Rorug02G0307000
rosa_samantha	Rh2AG359600 Rh2DG382500
rosa_wichuraiana	Rw2G029250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	46
AccI	GTMKAC	1 cut(s)	676
AciI	CCGC	2 cut(s)	237, 1108
AclI	AACGTT	1 cut(s)	1151
AclWI	GGATC	5 cut(s)	254, 322, 814, 1114, 1193
AcsI	RAATTY	3 cut(s)	367, 457, 508
AcuI	CTGAAG	5 cut(s)	27, 149, 579, 984, 1095
AfaI	GTAC	7 cut(s)	574, 652, 815, 1030, 1061, 1245, 1307
AfiI	CCNNNNNNNGG	3 cut(s)	107, 301, 1170
AgeI	ACCGGT	1 cut(s)	871
AgsI	TTSAA	5 cut(s)	551, 563, 1094, 1219, 1303
AhlI	ACTAGT	1 cut(s)	425
AjnI	CCWGG	2 cut(s)	588, 1225
AjuI	GAANNNNNNNTTGG	2 cut(s)	25, 57
Alw21I	GWGCWC	1 cut(s)	547
Alw26I	GTCTC	2 cut(s)	409, 882
AlwI	GGATC	5 cut(s)	254, 322, 814, 1114, 1193
AoxI	GGCC	3 cut(s)	77, 145, 292
ApeKI	GCWGC	6 cut(s)	253, 388, 409, 448, 566, 739
ApoI	RAATTY	3 cut(s)	367, 457, 508
ArsI	GACNNNNNNTTYG	2 cut(s)	485, 517
AsiGI	ACCGGT	1 cut(s)	871
AspA2I	CCTAGG	1 cut(s)	289
AspLEI	GCGC	2 cut(s)	21, 393
AsuHPI	GGTGA	2 cut(s)	449, 830
AvrII	CCTAGG	1 cut(s)	289
BanII	GRGCYC	1 cut(s)	547
BbsI	GAAGAC	3 cut(s)	100, 913, 1318
Bbv12I	GWGCWC	1 cut(s)	547
BbvI	GCAGC	6 cut(s)	240, 375, 421, 460, 553, 751
BccI	CCATC	3 cut(s)	749, 845, 1233
BciT130I	CCWGG	2 cut(s)	590, 1227
BciVI	GTATCC	1 cut(s)	285
BcoDI	GTCTC	2 cut(s)	409, 882
BcuI	ACTAGT	1 cut(s)	425
BfaI	CTAG	3 cut(s)	290, 426, 989
BfmI	CTRYAG	1 cut(s)	345
BfoI	RGCGCY	1 cut(s)	22
BfuAI	ACCTGC	1 cut(s)	46
BfuI	GTATCC	1 cut(s)	285
BisI	GCNGC	6 cut(s)	254, 389, 410, 449, 567, 740
BlnI	CCTAGG	1 cut(s)	289
BlsI	GCNGC	6 cut(s)	255, 390, 411, 450, 568, 741
BmcAI	AGTACT	1 cut(s)	1030
Bme1390I	CCNGG	2 cut(s)	590, 1227
BmrFI	CCNGG	2 cut(s)	590, 1227
BmrI	ACTGGG	1 cut(s)	457
BmsI	GCATC	2 cut(s)	52, 207
BmuI	ACTGGG	1 cut(s)	457
BpiI	GAAGAC	3 cut(s)	100, 913, 1318
BplI	GAGNNNNNCTC	2 cut(s)	623, 655
BpmI	CTGGAG	1 cut(s)	1278
BpuEI	CTTGAG	1 cut(s)	943
BsaAI	YACGTR	1 cut(s)	385
BsaJI	CCNNGG	2 cut(s)	289, 588
BsaWI	WCCGGW	1 cut(s)	871
BsaXI	ACNNNNNCTCC	4 cut(s)	102, 132, 472, 502
Bsc4I	CCNNNNNNNGG	3 cut(s)	107, 301, 1170
Bse118I	RCCGGY	1 cut(s)	871
Bse1I	ACTGG	5 cut(s)	463, 823, 1101, 1123, 1261
Bse3DI	GCAATG	1 cut(s)	927
BseBI	CCWGG	2 cut(s)	590, 1227
BseDI	CCNNGG	2 cut(s)	289, 588
BseGI	GGATG	4 cut(s)	512, 733, 754, 1200
BseLI	CCNNNNNNNGG	3 cut(s)	107, 301, 1170
BseMI	GCAATG	1 cut(s)	927
BseMII	CTCAG	2 cut(s)	70, 200
BseNI	ACTGG	5 cut(s)	463, 823, 1101, 1123, 1261
BseRI	GAGGAG	4 cut(s)	490, 493, 808, 949
BseXI	GCAGC	6 cut(s)	240, 375, 421, 460, 553, 751
BseYI	CCCAGC	1 cut(s)	1181
BsgI	GTGCAG	2 cut(s)	852, 990
BshFI	GGCC	3 cut(s)	79, 147, 294
BshTI	ACCGGT	1 cut(s)	871
BsiHKAI	GWGCWC	1 cut(s)	547
BsiSI	CCGG	1 cut(s)	872
BslI	CCNNNNNNNGG	3 cut(s)	107, 301, 1170
BsmAI	GTCTC	2 cut(s)	409, 882
BsmI	GAATGC	2 cut(s)	1232, 1286
BsnI	GGCC	3 cut(s)	79, 147, 294
Bsp1286I	GDGCHC	1 cut(s)	547
Bsp143I	GATC	7 cut(s)	259, 327, 645, 732, 806, 1119, 1185
BspACI	CCGC	2 cut(s)	237, 1108
BspANI	GGCC	3 cut(s)	79, 147, 294
BspCNI	CTCAG	2 cut(s)	69, 199
BspMAI	CTGCAG	1 cut(s)	349
BspMI	ACCTGC	1 cut(s)	46
BspPI	GGATC	5 cut(s)	254, 322, 814, 1114, 1193
BspQI	GCTCTTC	1 cut(s)	978
BsrDI	GCAATG	1 cut(s)	927
BsrFI	RCCGGY	1 cut(s)	871
BsrI	ACTGG	5 cut(s)	463, 823, 1101, 1123, 1261
BssAI	RCCGGY	1 cut(s)	871
BssECI	CCNNGG	2 cut(s)	289, 588
BssMI	GATC	7 cut(s)	259, 327, 645, 732, 806, 1119, 1185
BssT1I	CCWWGG	1 cut(s)	289
Bst2UI	CCWGG	2 cut(s)	590, 1227
Bst4CI	ACNGT	2 cut(s)	818, 950
Bst6I	CTCTTC	3 cut(s)	703, 785, 978
BstBAI	YACGTR	1 cut(s)	385
BstC8I	GCNNGC	2 cut(s)	23, 689
BstDEI	CTNAG	4 cut(s)	6, 56, 186, 319
BstEII	GGTNACC	1 cut(s)	818
BstF5I	GGATG	4 cut(s)	512, 733, 754, 1200
BstH2I	RGCGCY	1 cut(s)	22
BstHHI	GCGC	2 cut(s)	21, 393
BstKTI	GATC	7 cut(s)	262, 330, 648, 735, 809, 1122, 1188
BstMAI	GTCTC	2 cut(s)	409, 882
BstMBI	GATC	7 cut(s)	259, 327, 645, 732, 806, 1119, 1185
BstMWI	GCNNNNNNNGC	3 cut(s)	16, 563, 1114
BstNI	CCWGG	2 cut(s)	590, 1227
BstPI	GGTNACC	1 cut(s)	818
BstSCI	CCNGG	2 cut(s)	588, 1225
BstSFI	CTRYAG	1 cut(s)	345
BstV1I	GCAGC	6 cut(s)	240, 375, 421, 460, 553, 751
BstV2I	GAAGAC	3 cut(s)	100, 913, 1318
BstX2I	RGATCY	3 cut(s)	327, 806, 1185
BstYI	RGATCY	3 cut(s)	327, 806, 1185
BsuI	GTATCC	1 cut(s)	285
BsuRI	GGCC	3 cut(s)	79, 147, 294
BtsCI	GGATG	4 cut(s)	512, 733, 754, 1200
BtsIMutI	CAGTG	3 cut(s)	698, 955, 1071
BveI	ACCTGC	1 cut(s)	46
Cac8I	GCNNGC	2 cut(s)	23, 689
CfoI	GCGC	2 cut(s)	21, 393
Cfr10I	RCCGGY	1 cut(s)	871
Csp6I	GTAC	7 cut(s)	573, 651, 814, 1029, 1060, 1244, 1306
CspAI	ACCGGT	1 cut(s)	871
CviAII	CATG	3 cut(s)	193, 944, 1318
CviQI	GTAC	7 cut(s)	573, 651, 814, 1029, 1060, 1244, 1306
DdeI	CTNAG	4 cut(s)	6, 56, 186, 319
DpnI	GATC	7 cut(s)	261, 329, 647, 734, 808, 1121, 1187
DpnII	GATC	7 cut(s)	259, 327, 645, 732, 806, 1119, 1185
Eam1104I	CTCTTC	3 cut(s)	703, 785, 978
EarI	CTCTTC	3 cut(s)	703, 785, 978
Ecl136II	GAGCTC	1 cut(s)	545
Eco130I	CCWWGG	1 cut(s)	289
Eco147I	AGGCCT	1 cut(s)	147
Eco24I	GRGCYC	1 cut(s)	547
Eco53kI	GAGCTC	1 cut(s)	545
Eco57I	CTGAAG	5 cut(s)	27, 149, 579, 984, 1095
Eco91I	GGTNACC	1 cut(s)	818
EcoICRI	GAGCTC	1 cut(s)	545
EcoO65I	GGTNACC	1 cut(s)	818
EcoRI	GAATTC	2 cut(s)	367, 508
EcoRII	CCWGG	2 cut(s)	588, 1225
EcoT14I	CCWWGG	1 cut(s)	289
EcoT38I	GRGCYC	1 cut(s)	547
ErhI	CCWWGG	1 cut(s)	289
FaeI	CATG	3 cut(s)	196, 947, 1321
FalI	AAGNNNNNCTT	4 cut(s)	25, 57, 345, 377
FatI	CATG	3 cut(s)	192, 943, 1317
FauI	CCCGC	1 cut(s)	244
FblI	GTMKAC	1 cut(s)	676
Fnu4HI	GCNGC	6 cut(s)	254, 389, 410, 449, 567, 740
FokI	GGATG	4 cut(s)	499, 740, 761, 1207
FriOI	GRGCYC	1 cut(s)	547
Fsp4HI	GCNGC	6 cut(s)	254, 389, 410, 449, 567, 740
FspBI	CTAG	3 cut(s)	290, 426, 989
GlaI	GCGC	2 cut(s)	20, 392
GluI	GCNGC	6 cut(s)	254, 389, 410, 449, 567, 740
GsaI	CCCAGC	1 cut(s)	1185
GsuI	CTGGAG	1 cut(s)	1278
HaeII	RGCGCY	1 cut(s)	22
HaeIII	GGCC	3 cut(s)	79, 147, 294
HapII	CCGG	1 cut(s)	872
HhaI	GCGC	2 cut(s)	21, 393
Hin1II	CATG	3 cut(s)	196, 947, 1321
Hin6I	GCGC	2 cut(s)	19, 391
HinP1I	GCGC	2 cut(s)	19, 391
HincII	GTYRAC	3 cut(s)	202, 316, 1044
HindII	GTYRAC	3 cut(s)	202, 316, 1044
HindIII	AAGCTT	2 cut(s)	919, 1005
HinfI	GANTC	7 cut(s)	73, 160, 584, 899, 937, 1111, 1314
HpaII	CCGG	1 cut(s)	872
HphI	GGTGA	2 cut(s)	449, 830
Hpy166II	GTNNAC	7 cut(s)	140, 202, 210, 316, 653, 677, 1044
Hpy188I	TCNGA	9 cut(s)	7, 59, 129, 366, 598, 717, 964, 1140, 1238
Hpy188III	TCNNGA	5 cut(s)	90, 548, 581, 725, 903
Hpy8I	GTNNAC	7 cut(s)	140, 202, 210, 316, 653, 677, 1044
HpyAV	CCTTC	6 cut(s)	90, 113, 295, 712, 797, 959
HpyCH4III	ACNGT	2 cut(s)	818, 950
HpyCH4IV	ACGT	3 cut(s)	384, 575, 1151
HpyCH4V	TGCA	7 cut(s)	347, 409, 869, 953, 971, 1148, 1232
HpyF10VI	GCNNNNNNNGC	3 cut(s)	16, 563, 1114
HpyF3I	CTNAG	4 cut(s)	6, 56, 186, 319
HpySE526I	ACGT	3 cut(s)	384, 575, 1151
Hsp92II	CATG	3 cut(s)	196, 947, 1321
HspAI	GCGC	2 cut(s)	19, 391
Kzo9I	GATC	7 cut(s)	259, 327, 645, 732, 806, 1119, 1185
LguI	GCTCTTC	1 cut(s)	978
LmnI	GCTCC	2 cut(s)	480, 664
Lsp1109I	GCAGC	6 cut(s)	240, 375, 421, 460, 553, 751
LweI	GCATC	2 cut(s)	52, 207
MaeI	CTAG	3 cut(s)	290, 426, 989
MaeII	ACGT	3 cut(s)	384, 575, 1151
MaeIII	GTNAC	4 cut(s)	70, 227, 818, 836
MalI	GATC	7 cut(s)	261, 329, 647, 734, 808, 1121, 1187
MboI	GATC	7 cut(s)	259, 327, 645, 732, 806, 1119, 1185
MflI	RGATCY	3 cut(s)	327, 806, 1185
MhlI	GDGCHC	1 cut(s)	547
MluCI	AATT	4 cut(s)	367, 457, 508, 1251
MlyI	GAGTC	3 cut(s)	67, 946, 1308
MmeI	TCCRAC	1 cut(s)	582
MseI	TTAA	3 cut(s)	248, 1052, 1086
MslI	CAYNNNNRTG	3 cut(s)	215, 948, 1237
MspA1I	CMGCKG	1 cut(s)	1117
MspI	CCGG	1 cut(s)	872
MspR9I	CCNGG	2 cut(s)	590, 1227
Mva1269I	GAATGC	2 cut(s)	1232, 1286
MvaI	CCWGG	2 cut(s)	590, 1227
MwoI	GCNNNNNNNGC	3 cut(s)	16, 563, 1114
NdeII	GATC	7 cut(s)	259, 327, 645, 732, 806, 1119, 1185
NlaIII	CATG	3 cut(s)	196, 947, 1321
NmeAIII	GCCGAG	2 cut(s)	55, 320
NmuCI	GTSAC	3 cut(s)	70, 227, 818
PceI	AGGCCT	1 cut(s)	147
PciSI	GCTCTTC	1 cut(s)	978
PcsI	WCGNNNNNNNCGW	1 cut(s)	1148
PctI	GAATGC	2 cut(s)	1232, 1286
PfeI	GAWTC	4 cut(s)	160, 584, 899, 1111
PinAI	ACCGGT	1 cut(s)	871
PkrI	GCNGC	6 cut(s)	255, 390, 411, 450, 568, 741
PleI	GAGTC	3 cut(s)	67, 945, 1308
PpsI	GAGTC	3 cut(s)	67, 945, 1308
Ppu21I	YACGTR	1 cut(s)	385
Psp124BI	GAGCTC	1 cut(s)	547
Psp1406I	AACGTT	1 cut(s)	1151
Psp6I	CCWGG	2 cut(s)	588, 1225
PspEI	GGTNACC	1 cut(s)	818
PspFI	CCCAGC	1 cut(s)	1181
PspGI	CCWGG	2 cut(s)	588, 1225
PstI	CTGCAG	1 cut(s)	349
PsuI	RGATCY	3 cut(s)	327, 806, 1185
PvuII	CAGCTG	1 cut(s)	1117
RsaI	GTAC	7 cut(s)	574, 652, 815, 1030, 1061, 1245, 1307
RsaNI	GTAC	7 cut(s)	573, 651, 814, 1029, 1060, 1244, 1306
RseI	CAYNNNNRTG	3 cut(s)	215, 948, 1237
SacI	GAGCTC	1 cut(s)	547
SapI	GCTCTTC	1 cut(s)	978
SaqAI	TTAA	3 cut(s)	248, 1052, 1086
SatI	GCNGC	6 cut(s)	254, 389, 410, 449, 567, 740
Sau3AI	GATC	7 cut(s)	259, 327, 645, 732, 806, 1119, 1185
ScaI	AGTACT	1 cut(s)	1030
SchI	GAGTC	3 cut(s)	67, 946, 1308
ScrFI	CCNGG	2 cut(s)	590, 1227
SduI	GDGCHC	1 cut(s)	547
SfaNI	GCATC	2 cut(s)	52, 207
SfcI	CTRYAG	1 cut(s)	345
SgrAI	CRCCGGYG	1 cut(s)	871
SmiMI	CAYNNNNRTG	3 cut(s)	215, 948, 1237
SmlI	CTYRAG	1 cut(s)	922
SmoI	CTYRAG	1 cut(s)	922
SpeI	ACTAGT	1 cut(s)	425
Sse9I	AATT	4 cut(s)	367, 457, 508, 1251
SseBI	AGGCCT	1 cut(s)	147
SsiI	CCGC	2 cut(s)	237, 1108
SspMI	CTAG	3 cut(s)	290, 426, 989
SstI	GAGCTC	1 cut(s)	547
StuI	AGGCCT	1 cut(s)	147
StyD4I	CCNGG	2 cut(s)	588, 1225
StyI	CCWWGG	1 cut(s)	289
TaaI	ACNGT	2 cut(s)	818, 950
TaiI	ACGT	3 cut(s)	387, 578, 1154
TaqI	TCGA	4 cut(s)	534, 582, 735, 1128
TasI	AATT	4 cut(s)	367, 457, 508, 1251
TatI	WGTACW	4 cut(s)	650, 1028, 1059, 1305
TfiI	GAWTC	4 cut(s)	160, 584, 899, 1111
Tru1I	TTAA	3 cut(s)	248, 1052, 1086
Tru9I	TTAA	3 cut(s)	248, 1052, 1086
TscAI	CASTG	3 cut(s)	705, 955, 1078
TseFI	GTSAC	3 cut(s)	70, 227, 818
TseI	GCWGC	6 cut(s)	253, 388, 409, 448, 566, 739
Tsp45I	GTSAC	3 cut(s)	70, 227, 818
TspDTI	ATGAA	4 cut(s)	65, 501, 1072, 1211
TspGWI	ACGGA	1 cut(s)	320
TspRI	CASTG	3 cut(s)	705, 955, 1078
XapI	RAATTY	3 cut(s)	367, 457, 508
XmaJI	CCTAGG	1 cut(s)	289
XmiI	GTMKAC	1 cut(s)	676
XspI	CTAG	3 cut(s)	290, 426, 989
ZrmI	AGTACT	1 cut(s)	1030

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.