FvH4_6g28490

magnesium transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0007227
Physical Location & Seq
Forward (+)
21957229 .. 21961156
3928 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g28490.t2

Sequence Viewer

Length: 1341 bp
ATGGATGACATCAAAGAGCGGCTGCTTCCGCCAAAAGTTCCGAAACCTGCATCTGCTATTAATCTTCGAGATTCATCTTTTCGATCATCGGCCTTTGGGAGGCAACCTTTTCAAGGCGTGGATGTTCTGGGGCTGAAGAAGCGGGGCCAGGGCCTTCGGTCCTGGATTCGTGTTGATACATCTGGGAATTCTCAGATCATTGAGGTTGACAAGTTCACCATGATGCGACGTTGTGATTTACCTGCCCGTGATTTGCGCTTGCTTGATCCTCTATTTGTGTACCCCTCAACCATCCTTGGCAGAGAGAAGGCTATTGTAGTTAATCTGGAGCAGATCCGTTGTATTATCACGGCTGATGAGGTTCTGCTTTTGAATTCCCTTGATAACTATGTATTGCAGTATGTGGTGGAGCTACAGAAAAGATTGACAACAAGTGGTGTAGGTGATGTTTGGCAGTCTGATGGTTCTGACATGAGTAGGAGGAGGGGAGGTAGAAGTTTTGATAATGTGTTTGGGAGCACATCTCCTGACTATTTACCCTTTGAATTTAGGGCTTTAGAAGTTGCTCTGGAGGCTGCCTGTACATTCCTTGATTCTCAGGCAGCTGAACTGGAAATTGAAGCGTATCCATTACTTGATGAGCTTACGTCGAAAATCAGTACTCTGAACTTGGAACGTGCTCGACGATTGAAAAGCAGACTTCTTGCCTTGACTCGAAGAGTTCAGAAGGTTCGGGATGAAATAGAGCAGCTCATGGATGATGATGGAGATATGGCTGAGATGTATCTCACAGAGAAGAAAAGTCGTATGGAGGCAACATTTTATGGCGATCAATCTGTGATGGGGTATAGATCTAATGATGGTGTATCTCTTTCTGCTCCTGTTTCCCCTGTATCTTCACCTCCCGATGGTCGAAGACTTGAGAAAAGCTTGAGTATTGCAAGGAGTCGACATGAAAGCATGAGAAGTTCAGAAAGTGTTACAGAGAGTATAGAAGAGCTTGAGATGCTGCTGGAAGCATACTTTGTTGTCATTGATAGCACCCTGAATAAGCTGACTTCGTTGAAAGAATACATTGATGACACAGAAGATTTCATTAACATTCAGCTGGATAACGTACGTAATCAGCTGATTCAATTTGAGCTGCTACTAACAACTGCAACGTTTGTGGTTGCGATATTTGGAGTTGTAGCAGGAATCTTTGGCATGAACTTCGAAATCCCATTGTTTGATGACCCTGGCGCTTTTAAGTGGGTACTTGTAATCACAGGAGTAACTGGCATATGCATATTTTCTGCATTTGTGTGGTTCTTCAAGTATAGAAGACTCATGCCACTATAG

Protein Analysis

447

Amino Acids

50.51

Weight (kDa)

5.06

Isoelectric Point (pI)

57.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MRS2-like PF22099 61 - 269 4.5e-25 Magnesium transporter MRS2-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 55, 250
AccBSI CCGCTC 1 cut(s) 19
AccI GTMKAC 1 cut(s) 949
AciI CCGC 3 cut(s) 19, 29, 142
AclI AACGTT 1 cut(s) 1163
AclWI GGATC 2 cut(s) 260, 328
AcsI RAATTY 3 cut(s) 187, 373, 545
AcuI CTGAAG 1 cut(s) 155
AfaI GTAC 5 cut(s) 281, 583, 661, 1119, 1257
AfiI CCNNNNNNNGG 3 cut(s) 99, 113, 908
AgsI TTSAA 8 cut(s) 113, 373, 545, 620, 691, 1066, 1136, 1315
AjnI CCWGG 3 cut(s) 147, 161, 1237
Alw21I GWGCWC 2 cut(s) 521, 682
AlwI GGATC 2 cut(s) 260, 328
AoxI GGCC 3 cut(s) 90, 145, 151
ApeKI GCWGC 6 cut(s) 22, 575, 602, 748, 1009, 1144
ApoI RAATTY 3 cut(s) 187, 373, 545
AseI ATTAAT 1 cut(s) 60
AspLEI GCGC 2 cut(s) 258, 1244
AspS9I GGNCC 3 cut(s) 145, 151, 159
AsuHPI GGTGA 3 cut(s) 208, 455, 891
AsuII TTCGAA 1 cut(s) 1215
AvaII GGWCC 1 cut(s) 159
BbsI GAAGAC 2 cut(s) 922, 1330
Bbv12I GWGCWC 2 cut(s) 521, 682
BbvI GCAGC 6 cut(s) 9, 562, 614, 760, 996, 1131
BccI CCATC 6 cut(s) 299, 455, 758, 835, 854, 902
BceAI ACGGC 1 cut(s) 366
BcgI CGANNNNNNTGC 2 cut(s) 1195, 1229
BciT130I CCWGG 3 cut(s) 149, 163, 1239
BciVI GTATCC 1 cut(s) 636
BfmI CTRYAG 2 cut(s) 413, 1337
BfoI RGCGCY 1 cut(s) 1245
BfuAI ACCTGC 2 cut(s) 55, 250
BfuI GTATCC 1 cut(s) 636
BglII AGATCT 1 cut(s) 851
BisI GCNGC 7 cut(s) 20, 23, 576, 603, 749, 1010, 1145
BlsI GCNGC 7 cut(s) 21, 24, 577, 604, 750, 1011, 1146
BmcAI AGTACT 1 cut(s) 661
Bme1390I CCNGG 3 cut(s) 149, 163, 1239
Bme18I GGWCC 1 cut(s) 159
BmgT120I GGNCC 3 cut(s) 145, 151, 159
BmiI GGNNCC 1 cut(s) 146
BmrFI CCNGG 3 cut(s) 149, 163, 1239
BmsI GCATC 3 cut(s) 59, 213, 996
BpiI GAAGAC 2 cut(s) 922, 1330
BplI GAGNNNNNCTC 2 cut(s) 508, 540
BpmI CTGGAG 2 cut(s) 347, 590
Bpu14I TTCGAA 1 cut(s) 1215
BpuEI CTTGAG 3 cut(s) 941, 952, 1022
BsaAI YACGTR 1 cut(s) 1121
BsaJI CCNNGG 3 cut(s) 148, 295, 1237
BsaXI ACNNNNNCTCC 2 cut(s) 475, 505
Bsc4I CCNNNNNNNGG 3 cut(s) 99, 113, 908
Bse1I ACTGG 2 cut(s) 615, 1282
BseBI CCWGG 3 cut(s) 149, 163, 1239
BseDI CCNNGG 3 cut(s) 148, 295, 1237
BseGI GGATG 5 cut(s) 10, 127, 291, 742, 763
BseLI CCNNNNNNNGG 3 cut(s) 99, 113, 908
BseMII CTCAG 3 cut(s) 206, 611, 768
BseNI ACTGG 2 cut(s) 615, 1282
BseRI GAGGAG 1 cut(s) 496
BseXI GCAGC 6 cut(s) 9, 562, 614, 760, 996, 1131
BshFI GGCC 3 cut(s) 92, 147, 153
BsiHKAI GWGCWC 2 cut(s) 521, 682
BsiWI CGTACG 1 cut(s) 1117
BslI CCNNNNNNNGG 3 cut(s) 99, 113, 908
BsnI GGCC 3 cut(s) 92, 147, 153
Bsp119I TTCGAA 1 cut(s) 1215
Bsp1286I GDGCHC 2 cut(s) 521, 682
Bsp1407I TGTACA 1 cut(s) 581
Bsp143I GATC 6 cut(s) 83, 195, 265, 333, 829, 851
BspACI CCGC 3 cut(s) 19, 29, 142
BspANI GGCC 3 cut(s) 92, 147, 153
BspCNI CTCAG 3 cut(s) 205, 610, 769
BspLI GGNNCC 1 cut(s) 146
BspMI ACCTGC 2 cut(s) 55, 250
BspPI GGATC 2 cut(s) 260, 328
BspQI GCTCTTC 1 cut(s) 990
BspT104I TTCGAA 1 cut(s) 1215
BsrBI CCGCTC 1 cut(s) 19
BsrGI TGTACA 1 cut(s) 581
BsrI ACTGG 2 cut(s) 615, 1282
BssECI CCNNGG 3 cut(s) 148, 295, 1237
BssMI GATC 6 cut(s) 83, 195, 265, 333, 829, 851
BssT1I CCWWGG 1 cut(s) 295
Bst2UI CCWGG 3 cut(s) 149, 163, 1239
Bst6I CTCTTC 2 cut(s) 712, 990
BstAUI TGTACA 1 cut(s) 581
BstBAI YACGTR 1 cut(s) 1121
BstBI TTCGAA 1 cut(s) 1215
BstC8I GCNNGC 1 cut(s) 260
BstDEI CTNAG 3 cut(s) 192, 597, 777
BstENI CCTNNNNNAGG 2 cut(s) 97, 111
BstF5I GGATG 5 cut(s) 10, 127, 291, 742, 763
BstH2I RGCGCY 1 cut(s) 1245
BstHHI GCGC 2 cut(s) 258, 1244
BstKTI GATC 6 cut(s) 86, 198, 268, 336, 832, 854
BstMBI GATC 6 cut(s) 83, 195, 265, 333, 829, 851
BstMWI GCNNNNNNNGC 4 cut(s) 28, 139, 572, 1006
BstNI CCWGG 3 cut(s) 149, 163, 1239
BstSCI CCNGG 3 cut(s) 147, 161, 1237
BstSFI CTRYAG 2 cut(s) 413, 1337
BstSNI TACGTA 1 cut(s) 1121
BstV1I GCAGC 6 cut(s) 9, 562, 614, 760, 996, 1131
BstV2I GAAGAC 2 cut(s) 922, 1330
BstX2I RGATCY 2 cut(s) 333, 851
BstYI RGATCY 2 cut(s) 333, 851
BsuI GTATCC 1 cut(s) 636
BsuRI GGCC 3 cut(s) 92, 147, 153
BtsCI GGATG 5 cut(s) 10, 127, 291, 742, 763
BveI ACCTGC 2 cut(s) 55, 250
Cac8I GCNNGC 1 cut(s) 260
CfoI GCGC 2 cut(s) 258, 1244
Cfr13I GGNCC 3 cut(s) 145, 151, 159
Csp6I GTAC 5 cut(s) 280, 582, 660, 1118, 1256
CspCI CAANNNNNGTGG 2 cut(s) 1149, 1184
CviAII CATG 7 cut(s) 220, 472, 754, 953, 961, 1207, 1330
CviQI GTAC 5 cut(s) 280, 582, 660, 1118, 1256
DdeI CTNAG 3 cut(s) 192, 597, 777
DpnI GATC 6 cut(s) 85, 197, 267, 335, 831, 853
DpnII GATC 6 cut(s) 83, 195, 265, 333, 829, 851
Eam1104I CTCTTC 2 cut(s) 712, 990
EarI CTCTTC 2 cut(s) 712, 990
EciI GGCGGA 1 cut(s) 18
Eco105I TACGTA 1 cut(s) 1121
Eco130I CCWWGG 1 cut(s) 295
Eco47I GGWCC 1 cut(s) 159
Eco57I CTGAAG 1 cut(s) 155
EcoNI CCTNNNNNAGG 2 cut(s) 97, 111
EcoO109I RGGNCCY 1 cut(s) 151
EcoRI GAATTC 2 cut(s) 187, 373
EcoRII CCWGG 3 cut(s) 147, 161, 1237
EcoT14I CCWWGG 1 cut(s) 295
EcoT22I ATGCAT 1 cut(s) 1289
ErhI CCWWGG 1 cut(s) 295
FaeI CATG 7 cut(s) 223, 475, 757, 956, 964, 1210, 1333
FatI CATG 7 cut(s) 219, 471, 753, 952, 960, 1206, 1329
FauI CCCGC 1 cut(s) 135
FauNDI CATATG 1 cut(s) 1283
FblI GTMKAC 1 cut(s) 949
Fnu4HI GCNGC 7 cut(s) 20, 23, 576, 603, 749, 1010, 1145
FokI GGATG 5 cut(s) 17, 134, 278, 749, 770
Fsp4HI GCNGC 7 cut(s) 20, 23, 576, 603, 749, 1010, 1145
GlaI GCGC 2 cut(s) 257, 1243
GluI GCNGC 7 cut(s) 20, 23, 576, 603, 749, 1010, 1145
GsuI CTGGAG 2 cut(s) 347, 590
HaeII RGCGCY 1 cut(s) 1245
HaeIII GGCC 3 cut(s) 92, 147, 153
HhaI GCGC 2 cut(s) 258, 1244
Hin1II CATG 7 cut(s) 223, 475, 757, 956, 964, 1210, 1333
Hin6I GCGC 2 cut(s) 256, 1242
HinP1I GCGC 2 cut(s) 256, 1242
HincII GTYRAC 2 cut(s) 208, 950
HindII GTYRAC 2 cut(s) 208, 950
HindIII AAGCTT 1 cut(s) 928
HinfI GANTC 8 cut(s) 71, 166, 593, 712, 946, 1132, 1197, 1326
HphI GGTGA 3 cut(s) 208, 455, 891
Hpy166II GTNNAC 4 cut(s) 208, 216, 280, 950
Hpy188I TCNGA 7 cut(s) 42, 195, 460, 469, 666, 726, 973
Hpy188III TCNNGA 6 cut(s) 68, 326, 527, 569, 734, 905
Hpy8I GTNNAC 4 cut(s) 208, 216, 280, 950
Hpy99I CGWCG 3 cut(s) 231, 652, 687
HpyAV CCTTC 3 cut(s) 164, 301, 721
HpyCH4IV ACGT 6 cut(s) 229, 647, 676, 1116, 1120, 1163
HpyCH4V TGCA 6 cut(s) 50, 397, 941, 1160, 1287, 1298
HpyF10VI GCNNNNNNNGC 4 cut(s) 28, 139, 572, 1006
HpyF3I CTNAG 3 cut(s) 192, 597, 777
HpySE526I ACGT 6 cut(s) 229, 647, 676, 1116, 1120, 1163
Hsp92II CATG 7 cut(s) 223, 475, 757, 956, 964, 1210, 1333
HspAI GCGC 2 cut(s) 256, 1242
Kzo9I GATC 6 cut(s) 83, 195, 265, 333, 829, 851
LguI GCTCTTC 1 cut(s) 990
LmnI GCTCC 4 cut(s) 328, 409, 516, 883
Lsp1109I GCAGC 6 cut(s) 9, 562, 614, 760, 996, 1131
LweI GCATC 3 cut(s) 59, 213, 996
MaeII ACGT 6 cut(s) 229, 647, 676, 1116, 1120, 1163
MaeIII GTNAC 2 cut(s) 979, 1273
MalI GATC 6 cut(s) 85, 197, 267, 335, 831, 853
MbiI CCGCTC 1 cut(s) 19
MboI GATC 6 cut(s) 83, 195, 265, 333, 829, 851
MflI RGATCY 2 cut(s) 333, 851
MhlI GDGCHC 2 cut(s) 521, 682
MluCI AATT 5 cut(s) 187, 373, 545, 615, 1136
MlyI GAGTC 3 cut(s) 706, 955, 1320
Mph1103I ATGCAT 1 cut(s) 1289
MseI TTAA 4 cut(s) 60, 321, 1098, 1248
MslI CAYNNNNRTG 2 cut(s) 221, 1303
MspA1I CMGCKG 3 cut(s) 605, 1108, 1129
MspR9I CCNGG 3 cut(s) 149, 163, 1239
MvaI CCWGG 3 cut(s) 149, 163, 1239
MwoI GCNNNNNNNGC 4 cut(s) 28, 139, 572, 1006
NdeI CATATG 1 cut(s) 1283
NdeII GATC 6 cut(s) 83, 195, 265, 333, 829, 851
NlaIII CATG 7 cut(s) 223, 475, 757, 956, 964, 1210, 1333
NlaIV GGNNCC 1 cut(s) 146
NsiI ATGCAT 1 cut(s) 1289
NspV TTCGAA 1 cut(s) 1215
PciSI GCTCTTC 1 cut(s) 990
PcsI WCGNNNNNNNCGW 1 cut(s) 682
PfeI GAWTC 5 cut(s) 71, 166, 593, 1132, 1197
Pfl23II CGTACG 1 cut(s) 1117
PfoI TCCNGGA 1 cut(s) 161
PkrI GCNGC 7 cut(s) 21, 24, 577, 604, 750, 1011, 1146
PleI GAGTC 3 cut(s) 706, 954, 1320
PpsI GAGTC 3 cut(s) 706, 954, 1320
Ppu21I YACGTR 1 cut(s) 1121
PshBI ATTAAT 1 cut(s) 60
Psp1406I AACGTT 1 cut(s) 1163
Psp6I CCWGG 3 cut(s) 147, 161, 1237
PspGI CCWGG 3 cut(s) 147, 161, 1237
PspLI CGTACG 1 cut(s) 1117
PspN4I GGNNCC 1 cut(s) 146
PspPI GGNCC 3 cut(s) 145, 151, 159
PsuI RGATCY 2 cut(s) 333, 851
PvuII CAGCTG 3 cut(s) 605, 1108, 1129
RsaI GTAC 5 cut(s) 281, 583, 661, 1119, 1257
RsaNI GTAC 5 cut(s) 280, 582, 660, 1118, 1256
RseI CAYNNNNRTG 2 cut(s) 221, 1303
SalI GTCGAC 1 cut(s) 948
SapI GCTCTTC 1 cut(s) 990
SaqAI TTAA 4 cut(s) 60, 321, 1098, 1248
SatI GCNGC 7 cut(s) 20, 23, 576, 603, 749, 1010, 1145
Sau3AI GATC 6 cut(s) 83, 195, 265, 333, 829, 851
Sau96I GGNCC 3 cut(s) 145, 151, 159
ScaI AGTACT 1 cut(s) 661
SchI GAGTC 3 cut(s) 706, 955, 1320
ScrFI CCNGG 3 cut(s) 149, 163, 1239
SduI GDGCHC 2 cut(s) 521, 682
SfaNI GCATC 3 cut(s) 59, 213, 996
SfcI CTRYAG 2 cut(s) 413, 1337
SfuI TTCGAA 1 cut(s) 1215
SinI GGWCC 1 cut(s) 159
SmiMI CAYNNNNRTG 2 cut(s) 221, 1303
SmlI CTYRAG 3 cut(s) 920, 931, 1001
SmoI CTYRAG 3 cut(s) 920, 931, 1001
SnaBI TACGTA 1 cut(s) 1121
Sse9I AATT 5 cut(s) 187, 373, 545, 615, 1136
SsiI CCGC 3 cut(s) 19, 29, 142
StyD4I CCNGG 3 cut(s) 147, 161, 1237
StyI CCWWGG 1 cut(s) 295
TaiI ACGT 6 cut(s) 232, 650, 679, 1119, 1123, 1166
TaqI TCGA 8 cut(s) 67, 82, 650, 682, 715, 913, 949, 1215
TaqII GACCGA 1 cut(s) 147
TasI AATT 5 cut(s) 187, 373, 545, 615, 1136
TatI WGTACW 2 cut(s) 581, 659
TauI GCSGC 1 cut(s) 22
TfiI GAWTC 5 cut(s) 71, 166, 593, 1132, 1197
Tru1I TTAA 4 cut(s) 60, 321, 1098, 1248
Tru9I TTAA 4 cut(s) 60, 321, 1098, 1248
TseI GCWGC 6 cut(s) 22, 575, 602, 748, 1009, 1144
TspDTI ATGAA 5 cut(s) 63, 753, 969, 1084, 1223
TspGWI ACGGA 1 cut(s) 326
VpaK11BI GGWCC 1 cut(s) 159
VspI ATTAAT 1 cut(s) 60
XagI CCTNNNNNAGG 2 cut(s) 97, 111
XapI RAATTY 3 cut(s) 187, 373, 545
XmiI GTMKAC 1 cut(s) 949
ZrmI AGTACT 1 cut(s) 661
Zsp2I ATGCAT 1 cut(s) 1289
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.