Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr9

Orthogroup ID

OG0007227

Physical Location & Seq

Genomic Coordinates Reverse (-)

14174179 .. 14177921

Gene Structure 3743 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom09g14520.4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1341 bp

ATGGAGGACCTCAAAGAAAGGCTGCTTCCACCAAAAGTCCCAAAACCTGCTTCAGCTGTAAACCTTCGCGAAGCATCTTATCGACCATCCGCACTTGGAAGGCAACCTTTTCAAAGTGTGGATGTTCTGGGGCTGAAGAAGCGGGGCCAGGGTCTTCGGTCCTGGATTCGTGTTGATACGTCTGGCAATTCTCAGATCATTGAGGTTGATAAGTTCACTATGATGCGTCGTTGTGATCTTCCTGCCCGTGACCTACGGCTGCTTGATCCTCTATTTGTTTATCCTTCAACCATCCTTGGAAGAGAGAAAGCTATTGTAGTTAATCTAGAGCAGATTCGATGTATTATTACGGCCGATGAGGTTCTACTTTTGAATTCCCTTGATAGCTATGTATTACAGTATGTGGTGGAGCTTCAGAGAAGATTAACAACAAATGGAGTAGGTGATGTTTGGCAGTCTGATGGTTCTGACTTAAATCGGCTGAGGGGAAATAGAAATTTTGATAGTGTGTTTGGGAGCACATCTCCTGATTATTTGCCCTTTGAGTTTAGGGCTTTAGAAGTTGCTCTTGAGGCTGCCTGTACATTTCTTGATTCTCAGGCAGCCGAATTAGAAATTGAAGCATATCCATTACTGGACGAGCTCACGTCAAAGATCAGTACACTGAACTTGGAACGTGCTCGTCGTTTGAAAAGCAGACTTGTTGCCTTGACCCGGAGAGTTCAGAAGGTTCGTGATGAAATAGAGCAGCTCATGGATGATGATGGAGATATGGCTGAGATGTACCTGACTGAGAAGAAAAGTCGCATGGAGTCGGCATTTTATGGCGATCAATCTATGATGGGGTACAGATCTACTGATGGTGCATCTCTTTCTGCTCCTGTTTCTCCTGTATCTTCACCTCCCGATGGCCGAAAGCTTGAGAAAAGCCTCAGCATTGCAAGGAGTAGACATGAAAGCATGAGGAGTTCAGATAGTGCTACAGAGAGTATAGAAGAGCTTGAGATGTTGCTGGAAGCATACTTCGTCGTCATCGATAGCACCCTGAATAAGCTGACATCGTTGAAAGAGTACATTGACGACACTGAAGATTTCATTAACATTCAACTGGATAACGTTCGAAACCAGCTGATTCAATTCGAGCTACTGCTCACAACTGCGACGTTTGTTGTTGCGATGTTTGGGGTTGTGGCCGGAATCTTTGGGATGAACTTCGCAATACCGATGTTTGATGAGCCTGCTGCATTTAAGTGGGTACTTATAATTACAGGAGTAACTGGCTTTTGCATATTTTCTGCATTTGTGTGGTTCTTCAAGTATCGAAGACTGATGCAACTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0008324 GO:0015075 GO:0015095 GO:0015318 GO:0015693 GO:0022857 GO:0022890 GO:0030001 GO:0034220 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0070838 GO:0072511 GO:0098655 GO:0098660 GO:0098662 GO:1903830

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CorA

Protein Analysis

447

Amino Acids

50.55

Weight (kDa)

5.06

Isoelectric Point (pI)

50.3

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007227)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G16010 AT1G16010 AT1G16010 AT1G80900 AT1G80900
fragaria_vesca	FvH4_6g28490
malus_domestica	MD09G1225700.v1.1 MD17G1224500.v1.1
prunus_persica	Prupe.3G100200_v2.0.a1 Prupe.3G100200_v2.0.a1 Prupe.3G100200_v2.0.a1 Prupe.3G100200_v2.0.a1 Prupe.3G100200_v2.0.a1
pyrus_communis	pycom09g14520 pycom17g22820
rosa_chinensis	RchiOBHm_Chr2g0132741
rosa_laevigata	RLG00000019274
rosa_multiflora	Rmu_sc0004972.1_g000001 Rmu_ssc0000074.1_g000006
rosa_roxburghii	Rroxscaffold_2G00111690
rosa_rugosa	Rorug02G0307000 Rorug02G0307000
rosa_samantha	Rh2AG359600 Rh2DG382500
rosa_wichuraiana	Rw2G029250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1262
Acc36I	ACCTGC	1 cut(s)	55
AccI	GTMKAC	1 cut(s)	949
AccII	CGCG	1 cut(s)	69
AciI	CCGC	2 cut(s)	90, 142
AclI	AACGTT	1 cut(s)	1116
AclWI	GGATC	1 cut(s)	260
AcoI	YGGCCR	3 cut(s)	351, 910, 1191
AcsI	RAATTY	2 cut(s)	373, 496
AcuI	CTGAAG	4 cut(s)	36, 155, 398, 1107
AfaI	GTAC	6 cut(s)	583, 661, 785, 848, 1073, 1257
AfiI	CCNNNNNNNGG	2 cut(s)	714, 908
AgsI	TTSAA	9 cut(s)	113, 288, 373, 620, 691, 1066, 1106, 1136, 1315
AjiI	CACGTC	1 cut(s)	648
AjnI	CCWGG	2 cut(s)	147, 161
AjuI	GAANNNNNNNTTGG	2 cut(s)	34, 66
Alw21I	GWGCWC	3 cut(s)	521, 645, 682
AlwI	GGATC	1 cut(s)	260
AoxI	GGCC	4 cut(s)	145, 351, 910, 1191
ApeKI	GCWGC	6 cut(s)	22, 259, 575, 602, 748, 1241
ApoI	RAATTY	2 cut(s)	373, 496
AspS9I	GGNCC	3 cut(s)	7, 145, 159
AsuC2I	CCSGG	1 cut(s)	715
AsuHPI	GGTGA	2 cut(s)	455, 891
AsuII	TTCGAA	1 cut(s)	1120
AvaII	GGWCC	2 cut(s)	7, 159
BanII	GRGCYC	1 cut(s)	645
BbsI	GAAGAC	2 cut(s)	146, 1330
Bbv12I	GWGCWC	3 cut(s)	521, 645, 682
BbvCI	CCTCAGC	2 cut(s)	482, 932
BbvI	GCAGC	6 cut(s)	9, 246, 562, 614, 760, 1228
BccI	CCATC	7 cut(s)	94, 299, 455, 758, 835, 854, 902
BceAI	ACGGC	2 cut(s)	272, 366
BciT130I	CCWGG	2 cut(s)	149, 163
BcnI	CCSGG	1 cut(s)	715
BfaI	CTAG	1 cut(s)	326
BfmI	CTRYAG	2 cut(s)	981, 1337
BfuAI	ACCTGC	1 cut(s)	55
BglII	AGATCT	1 cut(s)	851
BisI	GCNGC	6 cut(s)	23, 260, 576, 603, 749, 1242
BlsI	GCNGC	6 cut(s)	24, 261, 577, 604, 750, 1243
Bme1390I	CCNGG	3 cut(s)	149, 163, 715
Bme18I	GGWCC	2 cut(s)	7, 159
BmgBI	CACGTC	1 cut(s)	648
BmgT120I	GGNCC	3 cut(s)	7, 145, 159
BmiI	GGNNCC	1 cut(s)	146
BmrFI	CCNGG	3 cut(s)	149, 163, 715
BmsI	GCATC	4 cut(s)	83, 213, 875, 1320
BpiI	GAAGAC	2 cut(s)	146, 1330
BplI	GAGNNNNNCTC	2 cut(s)	508, 540
Bpu10I	CCTNAGC	2 cut(s)	482, 932
Bpu14I	TTCGAA	1 cut(s)	1120
BpuEI	CTTGAG	3 cut(s)	590, 941, 1022
BpuMI	CCSGG	1 cut(s)	715
Bsa29I	ATCGAT	1 cut(s)	1035
BsaJI	CCNNGG	2 cut(s)	148, 295
Bsc4I	CCNNNNNNNGG	2 cut(s)	714, 908
Bse1I	ACTGG	3 cut(s)	639, 1113, 1282
Bse3DI	GCAATG	1 cut(s)	936
BseBI	CCWGG	2 cut(s)	149, 163
BseCI	ATCGAT	1 cut(s)	1035
BseDI	CCNNGG	2 cut(s)	148, 295
BseGI	GGATG	5 cut(s)	86, 127, 291, 763, 1212
BseLI	CCNNNNNNNGG	2 cut(s)	714, 908
BseMI	GCAATG	1 cut(s)	936
BseMII	CTCAG	6 cut(s)	206, 473, 611, 768, 783, 946
BseNI	ACTGG	3 cut(s)	639, 1113, 1282
BseRI	GAGGAG	1 cut(s)	979
BseX3I	CGGCCG	1 cut(s)	351
BseXI	GCAGC	6 cut(s)	9, 246, 562, 614, 760, 1228
Bsh1236I	CGCG	1 cut(s)	69
Bsh1285I	CGRYCG	1 cut(s)	354
BshFI	GGCC	4 cut(s)	147, 353, 912, 1193
BshVI	ATCGAT	1 cut(s)	1035
BsiEI	CGRYCG	1 cut(s)	354
BsiHKAI	GWGCWC	3 cut(s)	521, 645, 682
BsiSI	CCGG	2 cut(s)	715, 1194
BslFI	GGGAC	1 cut(s)	23
BslI	CCNNNNNNNGG	2 cut(s)	714, 908
BsmFI	GGGAC	1 cut(s)	23
BsnI	GGCC	4 cut(s)	147, 353, 912, 1193
Bsp119I	TTCGAA	1 cut(s)	1120
Bsp1286I	GDGCHC	3 cut(s)	521, 645, 682
Bsp1407I	TGTACA	1 cut(s)	581
Bsp143I	GATC	6 cut(s)	195, 235, 265, 654, 829, 851
Bsp68I	TCGCGA	1 cut(s)	69
BspACI	CCGC	2 cut(s)	90, 142
BspANI	GGCC	4 cut(s)	147, 353, 912, 1193
BspCNI	CTCAG	6 cut(s)	205, 474, 610, 769, 784, 945
BspDI	ATCGAT	1 cut(s)	1035
BspFNI	CGCG	1 cut(s)	69
BspLI	GGNNCC	1 cut(s)	146
BspMI	ACCTGC	1 cut(s)	55
BspPI	GGATC	1 cut(s)	260
BspQI	GCTCTTC	1 cut(s)	990
BspT104I	TTCGAA	1 cut(s)	1120
BsrDI	GCAATG	1 cut(s)	936
BsrGI	TGTACA	1 cut(s)	581
BsrI	ACTGG	3 cut(s)	639, 1113, 1282
BssECI	CCNNGG	2 cut(s)	148, 295
BssMI	GATC	6 cut(s)	195, 235, 265, 654, 829, 851
BssT1I	CCWWGG	1 cut(s)	295
Bst2UI	CCWGG	2 cut(s)	149, 163
Bst4CI	ACNGT	1 cut(s)	399
Bst6I	CTCTTC	2 cut(s)	295, 990
BstAUI	TGTACA	1 cut(s)	581
BstBI	TTCGAA	1 cut(s)	1120
BstC8I	GCNNGC	1 cut(s)	1239
BstDEI	CTNAG	6 cut(s)	192, 482, 597, 777, 792, 932
BstF5I	GGATG	5 cut(s)	86, 127, 291, 763, 1212
BstFNI	CGCG	1 cut(s)	69
BstKTI	GATC	6 cut(s)	198, 238, 268, 657, 832, 854
BstMBI	GATC	6 cut(s)	195, 235, 265, 654, 829, 851
BstMCI	CGRYCG	1 cut(s)	354
BstMWI	GCNNNNNNNGC	2 cut(s)	139, 572
BstNI	CCWGG	2 cut(s)	149, 163
BstSCI	CCNGG	3 cut(s)	147, 161, 713
BstSFI	CTRYAG	2 cut(s)	981, 1337
BstUI	CGCG	1 cut(s)	69
BstV1I	GCAGC	6 cut(s)	9, 246, 562, 614, 760, 1228
BstV2I	GAAGAC	2 cut(s)	146, 1330
BstX2I	RGATCY	1 cut(s)	851
BstYI	RGATCY	1 cut(s)	851
BstZI	CGGCCG	1 cut(s)	351
Bsu15I	ATCGAT	1 cut(s)	1035
BsuRI	GGCC	4 cut(s)	147, 353, 912, 1193
BsuTUI	ATCGAT	1 cut(s)	1035
BtgZI	GCGATG	1 cut(s)	1190
BtrI	CACGTC	1 cut(s)	648
BtsCI	GGATG	5 cut(s)	86, 127, 291, 763, 1212
BtsIMutI	CAGTG	2 cut(s)	662, 1083
BtuMI	TCGCGA	1 cut(s)	69
BveI	ACCTGC	1 cut(s)	55
Cac8I	GCNNGC	1 cut(s)	1239
Cfr13I	GGNCC	3 cut(s)	7, 145, 159
ClaI	ATCGAT	1 cut(s)	1035
CseI	GACGC	1 cut(s)	215
Csp6I	GTAC	6 cut(s)	582, 660, 784, 847, 1072, 1256
CviAII	CATG	4 cut(s)	754, 808, 953, 961
CviQI	GTAC	6 cut(s)	582, 660, 784, 847, 1072, 1256
DdeI	CTNAG	6 cut(s)	192, 482, 597, 777, 792, 932
DpnI	GATC	6 cut(s)	197, 237, 267, 656, 831, 853
DpnII	GATC	6 cut(s)	195, 235, 265, 654, 829, 851
EaeI	YGGCCR	3 cut(s)	351, 910, 1191
EagI	CGGCCG	1 cut(s)	351
Eam1104I	CTCTTC	2 cut(s)	295, 990
EarI	CTCTTC	2 cut(s)	295, 990
Ecl136II	GAGCTC	1 cut(s)	643
EclXI	CGGCCG	1 cut(s)	351
Eco130I	CCWWGG	1 cut(s)	295
Eco24I	GRGCYC	1 cut(s)	645
Eco47I	GGWCC	2 cut(s)	7, 159
Eco52I	CGGCCG	1 cut(s)	351
Eco53kI	GAGCTC	1 cut(s)	643
Eco57I	CTGAAG	4 cut(s)	36, 155, 398, 1107
EcoICRI	GAGCTC	1 cut(s)	643
EcoO109I	RGGNCCY	1 cut(s)	7
EcoRI	GAATTC	1 cut(s)	373
EcoRII	CCWGG	2 cut(s)	147, 161
EcoT14I	CCWWGG	1 cut(s)	295
EcoT38I	GRGCYC	1 cut(s)	645
ErhI	CCWWGG	1 cut(s)	295
FaeI	CATG	4 cut(s)	757, 811, 956, 964
FalI	AAGNNNNNCTT	4 cut(s)	91, 123, 552, 584
FaqI	GGGAC	1 cut(s)	23
FatI	CATG	4 cut(s)	753, 807, 952, 960
FauI	CCCGC	1 cut(s)	135
FblI	GTMKAC	1 cut(s)	949
Fnu4HI	GCNGC	6 cut(s)	23, 260, 576, 603, 749, 1242
FokI	GGATG	5 cut(s)	73, 134, 278, 770, 1219
FriOI	GRGCYC	1 cut(s)	645
Fsp4HI	GCNGC	6 cut(s)	23, 260, 576, 603, 749, 1242
FspBI	CTAG	1 cut(s)	326
GluI	GCNGC	6 cut(s)	23, 260, 576, 603, 749, 1242
HaeIII	GGCC	4 cut(s)	147, 353, 912, 1193
HapII	CCGG	2 cut(s)	715, 1194
HgaI	GACGC	1 cut(s)	215
Hin1II	CATG	4 cut(s)	757, 811, 956, 964
HindIII	AAGCTT	1 cut(s)	917
HinfI	GANTC	6 cut(s)	166, 334, 593, 812, 1132, 1197
HpaII	CCGG	2 cut(s)	715, 1194
HphI	GGTGA	2 cut(s)	455, 891
Hpy166II	GTNNAC	4 cut(s)	61, 216, 662, 950
Hpy188I	TCNGA	6 cut(s)	195, 417, 460, 469, 726, 973
Hpy188III	TCNNGA	7 cut(s)	68, 326, 527, 569, 590, 734, 905
Hpy8I	GTNNAC	4 cut(s)	61, 216, 662, 950
Hpy99I	CGWCG	4 cut(s)	231, 687, 1031, 1165
HpyAV	CCTTC	4 cut(s)	74, 93, 294, 721
HpyCH4III	ACNGT	1 cut(s)	399
HpyCH4IV	ACGT	5 cut(s)	179, 647, 676, 1116, 1163
HpyCH4V	TGCA	6 cut(s)	866, 941, 1244, 1287, 1298, 1333
HpyF10VI	GCNNNNNNNGC	2 cut(s)	139, 572
HpyF3I	CTNAG	6 cut(s)	192, 482, 597, 777, 792, 932
HpySE526I	ACGT	5 cut(s)	179, 647, 676, 1116, 1163
Hsp92II	CATG	4 cut(s)	757, 811, 956, 964
Kzo9I	GATC	6 cut(s)	195, 235, 265, 654, 829, 851
LguI	GCTCTTC	1 cut(s)	990
LmnI	GCTCC	3 cut(s)	409, 516, 883
Lsp1109I	GCAGC	6 cut(s)	9, 246, 562, 614, 760, 1228
LweI	GCATC	4 cut(s)	83, 213, 875, 1320
MaeI	CTAG	1 cut(s)	326
MaeII	ACGT	5 cut(s)	179, 647, 676, 1116, 1163
MaeIII	GTNAC	2 cut(s)	248, 1273
MalI	GATC	6 cut(s)	197, 237, 267, 656, 831, 853
MboI	GATC	6 cut(s)	195, 235, 265, 654, 829, 851
MflI	RGATCY	1 cut(s)	851
MhlI	GDGCHC	3 cut(s)	521, 645, 682
MluCI	AATT	7 cut(s)	187, 373, 496, 608, 615, 1136, 1263
MlyI	GAGTC	1 cut(s)	821
MnlI	CCTC	9 cut(s)	20, 196, 279, 352, 477, 565, 912, 941, 957
MseI	TTAA	5 cut(s)	321, 425, 473, 1098, 1248
MslI	CAYNNNNRTG	3 cut(s)	221, 1249, 1303
MspA1I	CMGCKG	2 cut(s)	56, 1129
MspI	CCGG	2 cut(s)	715, 1194
MspR9I	CCNGG	3 cut(s)	149, 163, 715
MvaI	CCWGG	2 cut(s)	149, 163
MvnI	CGCG	1 cut(s)	69
MwoI	GCNNNNNNNGC	2 cut(s)	139, 572
NciI	CCSGG	1 cut(s)	715
NdeII	GATC	6 cut(s)	195, 235, 265, 654, 829, 851
NlaIII	CATG	4 cut(s)	757, 811, 956, 964
NlaIV	GGNNCC	1 cut(s)	146
NmuCI	GTSAC	1 cut(s)	248
NruI	TCGCGA	1 cut(s)	69
NspV	TTCGAA	1 cut(s)	1120
PciSI	GCTCTTC	1 cut(s)	990
PcsI	WCGNNNNNNNCGW	2 cut(s)	682, 1032
PfeI	GAWTC	5 cut(s)	166, 334, 593, 1132, 1197
PfoI	TCCNGGA	1 cut(s)	161
PkrI	GCNGC	6 cut(s)	24, 261, 577, 604, 750, 1243
PleI	GAGTC	1 cut(s)	820
PpsI	GAGTC	1 cut(s)	820
PpuMI	RGGWCCY	1 cut(s)	7
PsiI	TTATAA	1 cut(s)	1262
Psp124BI	GAGCTC	1 cut(s)	645
Psp1406I	AACGTT	1 cut(s)	1116
Psp5II	RGGWCCY	1 cut(s)	7
Psp6I	CCWGG	2 cut(s)	147, 161
PspGI	CCWGG	2 cut(s)	147, 161
PspN4I	GGNNCC	1 cut(s)	146
PspPI	GGNCC	3 cut(s)	7, 145, 159
PspPPI	RGGWCCY	1 cut(s)	7
PsuI	RGATCY	1 cut(s)	851
PvuII	CAGCTG	2 cut(s)	56, 1129
RruI	TCGCGA	1 cut(s)	69
RsaI	GTAC	6 cut(s)	583, 661, 785, 848, 1073, 1257
RsaNI	GTAC	6 cut(s)	582, 660, 784, 847, 1072, 1256
RseI	CAYNNNNRTG	3 cut(s)	221, 1249, 1303
SacI	GAGCTC	1 cut(s)	645
SapI	GCTCTTC	1 cut(s)	990
SaqAI	TTAA	5 cut(s)	321, 425, 473, 1098, 1248
SatI	GCNGC	6 cut(s)	23, 260, 576, 603, 749, 1242
Sau3AI	GATC	6 cut(s)	195, 235, 265, 654, 829, 851
Sau96I	GGNCC	3 cut(s)	7, 145, 159
SchI	GAGTC	1 cut(s)	821
ScrFI	CCNGG	3 cut(s)	149, 163, 715
SduI	GDGCHC	3 cut(s)	521, 645, 682
SfaNI	GCATC	4 cut(s)	83, 213, 875, 1320
SfcI	CTRYAG	2 cut(s)	981, 1337
SfuI	TTCGAA	1 cut(s)	1120
SinI	GGWCC	2 cut(s)	7, 159
SmiMI	CAYNNNNRTG	3 cut(s)	221, 1249, 1303
SmlI	CTYRAG	3 cut(s)	569, 920, 1001
SmoI	CTYRAG	3 cut(s)	569, 920, 1001
Sse9I	AATT	7 cut(s)	187, 373, 496, 608, 615, 1136, 1263
SsiI	CCGC	2 cut(s)	90, 142
SspMI	CTAG	1 cut(s)	326
SstI	GAGCTC	1 cut(s)	645
StyD4I	CCNGG	3 cut(s)	147, 161, 713
StyI	CCWWGG	1 cut(s)	295
TaaI	ACNGT	1 cut(s)	399
TaiI	ACGT	5 cut(s)	182, 650, 679, 1119, 1166
TaqI	TCGA	6 cut(s)	82, 337, 1035, 1120, 1140, 1321
TaqII	GACCGA	1 cut(s)	147
TasI	AATT	7 cut(s)	187, 373, 496, 608, 615, 1136, 1263
TatI	WGTACW	3 cut(s)	581, 659, 1071
TfiI	GAWTC	5 cut(s)	166, 334, 593, 1132, 1197
Tru1I	TTAA	5 cut(s)	321, 425, 473, 1098, 1248
Tru9I	TTAA	5 cut(s)	321, 425, 473, 1098, 1248
TscAI	CASTG	2 cut(s)	669, 1090
TseFI	GTSAC	1 cut(s)	248
TseI	GCWGC	6 cut(s)	22, 259, 575, 602, 748, 1241
Tsp45I	GTSAC	1 cut(s)	248
TspDTI	ATGAA	4 cut(s)	753, 969, 1084, 1223
TspRI	CASTG	2 cut(s)	669, 1090
VpaK11BI	GGWCC	2 cut(s)	7, 159
XapI	RAATTY	2 cut(s)	373, 496
XbaI	TCTAGA	1 cut(s)	325
XmiI	GTMKAC	1 cut(s)	949
XspI	CTAG	1 cut(s)	326

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.