Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0010462

Physical Location & Seq

Genomic Coordinates Reverse (-)

5567742 .. 5570679

Gene Structure 2938 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G16280.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1476 bp

ATGGAGGAACCAACGCCGGAGGAGGAAGGGGGAATCACCATCATGTCTAAATCCCGCAAAAATCCTAAAACCGTAGTAAACATTCAGTCCCAGAAACTTGATTCGGACCAGAATACTCCTCAATTTGAGAAATTCACTAATCCTAACCCTAGCTCCGACACCACATCCGCCACTAATTTCGAAGGGCTCGGATTAGCTGAGTGGGCAGTGGAAACGTGTAAAGAGCTTGGGATGCGTAAACCCACTCCAGTTCAAACTCACTGCGTGCCGAAGATATTAGCCGGACGAGACGTTTTAGGGCTTGCTCAGACTGGTAGTGGTAAAACTGCTGCGTTTGCTCTACCTATACTTCACCGCCTTGCTGAAGATCCTTATGGAGTCTTCGCGCTTGTAGTGACGCCGACTAGAGAGCTAGCGTTCCAGCTCGCTGAGCAGTTTAAGGCTTTGGGTTCGTGTTTGAATTTGCGTTGCTCTGTAATTGTGGGAGGAATGGATATGTTGACGCAAACTATGAGCTTGGTTTCACGACCTCATATCGTTATTACTACTCCTGGAAGAATCAAGGTTTTGCTTGAAAACAATCCTGATGTTCCTCCAGTTTTCTCTAGAACCAAGTTCTTAGTGCTGGACGAGGCGGATAGAGTTCTTGATGTTGGTTTCCAAGACGAGTTACGCACAATCTTTCAGTGTCTGCCAAAGAGTAGACAAACATTGTTGTTCTCTGCTACCATGACTAGTAACTTACAGGCTTTGCTTGAACATTCTTCAAACAAAGCTTATTTCTATGAAGCCTATGAAGGGTTAAAGACTGTTGACACTCTTACGCAGCAGTTCATTTTCGAAGACAAAGATGCCAAAGAGCTGTATCTTGTGCACATCCTCTCGCAAATGGAAGACAAGGGCATTCGATCAGCCATGATTTTTGTCTCCACTTGCAGAACATGTCAAAGGTTAAGTCTTATGCTTGACGAGCTTGAAGTGGAAAATATAGCTATGCATTCTCTGAATTCTCAGTCTATGCGACTATCTGCACTAAGTAAATTCAAGTCTGGGAAAGTACCGATATTGCTTGCGACTGATGTTGCCAGCCGTGGATTGGACATTCCTACTGTTGACCTTGTCATCAATTATGACATTCCGAGAGATCCTAGGGATTATGTTCATCGTGTTGGACGTACAGCTAGAGCTGGTAGAGGAGGACTGGCTGTAAGCATTATCACTGAGACCGATGTGAAACTTATCCATAAAATAGAAGAGGAAGTTGGAAAAAAGATGGAACCATACAACAAAAAAGTAATCACTGACAGTTTAGAAGTCACAAAGGTCTCAAAAGCCAAAAGAGTGGCAATGATGAAGATGCTAGATAATGGATTCGAAGACAAGGTTAAAGACCGGAGGAAATTGAAACGGAAAACACTTGCGGATAAAGGGTTGCTTAAGAAACGGGGCAAAAGGCAGAAATCAACAGAAAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0007275 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009553 GO:0009561 GO:0009791 GO:0009987 GO:0010467 GO:0010501 GO:0016070 GO:0016072 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022613 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0042623 GO:0042995 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0048229 GO:0048856 GO:0070013 GO:0070035 GO:0071704 GO:0071840 GO:0090304 GO:0090406 GO:0120025 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

491

Amino Acids

54.83

Weight (kDa)

9.0

Isoelectric Point (pI)

36.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	82 - 250	2.5e-47	DEAD/DEAH box helicase
Helicase_C	PF00271	291 - 397	2.8e-27	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010462)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G16280
fragaria_vesca	FvH4_3g14750 FvH4_3g14750 FvH4_3g14750
malus_domestica	MD10G1209000.v1.1
prunus_persica	Prupe.4G132500_v2.0.a1
pyrus_communis	pycom10g17780 pycom13g14880
rosa_chinensis	RchiOBHm_Chr5g0024261
rosa_laevigata	RLG00000032788
rosa_multiflora	Rmu_sc0002344.1_g000004 Rmu_sc0018255.1_g000002
rosa_roxburghii	Rroxscaffold_1G00054920 Rroxscaffold_1G00055760
rosa_rugosa	Rorug05G0082900
rosa_samantha	Rh5AG173300 Rh5BG170200 Rh5CG186300 Rh5DG172800
rosa_wichuraiana	Rw0G000850 Rw5G015650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	703
AccII	CGCG	1 cut(s)	386
AciI	CCGC	5 cut(s)	55, 168, 355, 635, 1421
AclWI	GGATC	2 cut(s)	362, 1139
AcsI	RAATTY	4 cut(s)	131, 460, 1006, 1040
AcuI	CTGAAG	1 cut(s)	384
AcyI	GRCGYC	1 cut(s)	398
AdeI	CACNNNGTG	1 cut(s)	265
AfaI	GTAC	2 cut(s)	1059, 1177
AfiI	CCNNNNNNNGG	2 cut(s)	798, 1096
AflII	CTTAAG	1 cut(s)	1436
AflIII	ACRYGT	2 cut(s)	215, 941
AgsI	TTSAA	8 cut(s)	254, 460, 575, 758, 768, 977, 1045, 1405
AhlI	ACTAGT	1 cut(s)	734
AjnI	CCWGG	1 cut(s)	550
AjuI	GAANNNNNNNTTGG	2 cut(s)	1245, 1277
Alw21I	GWGCWC	1 cut(s)	876
Alw26I	GTCTC	4 cut(s)	282, 931, 1217, 1330
Alw44I	GTGCAC	1 cut(s)	872
AlwI	GGATC	2 cut(s)	362, 1139
AlwNI	CAGNNNCTG	1 cut(s)	691
ApaLI	GTGCAC	1 cut(s)	872
ApeKI	GCWGC	2 cut(s)	329, 826
ApoI	RAATTY	4 cut(s)	131, 460, 1006, 1040
ArsI	GACNNNNNNTTYG	2 cut(s)	940, 972
AspA2I	CCTAGG	1 cut(s)	1148
AspLEI	GCGC	1 cut(s)	388
AspS9I	GGNCC	1 cut(s)	106
AsuHPI	GGTGA	2 cut(s)	28, 344
AsuII	TTCGAA	3 cut(s)	180, 840, 1374
AsuNHI	GCTAGC	1 cut(s)	412
AvaII	GGWCC	1 cut(s)	106
AvrII	CCTAGG	1 cut(s)	1148
BaeGI	GKGCMC	1 cut(s)	876
BanII	GRGCYC	1 cut(s)	189
BbsI	GAAGAC	4 cut(s)	373, 849, 900, 1383
Bbv12I	GWGCWC	1 cut(s)	876
BbvI	GCAGC	2 cut(s)	316, 838
BccI	CCATC	2 cut(s)	47, 1267
BceAI	ACGGC	1 cut(s)	1074
BciT130I	CCWGG	1 cut(s)	552
BcoDI	GTCTC	4 cut(s)	282, 931, 1217, 1330
BcuI	ACTAGT	1 cut(s)	734
BfaI	CTAG	8 cut(s)	150, 405, 413, 606, 735, 1149, 1182, 1361
BfrI	CTTAAG	1 cut(s)	1436
BisI	GCNGC	2 cut(s)	330, 827
BlnI	CCTAGG	1 cut(s)	1148
BlpI	GCTNAGC	1 cut(s)	429
BlsI	GCNGC	2 cut(s)	331, 828
Bme1390I	CCNGG	1 cut(s)	552
Bme18I	GGWCC	1 cut(s)	106
BmgT120I	GGNCC	1 cut(s)	106
BmiI	GGNNCC	2 cut(s)	9, 1278
BmrFI	CCNGG	1 cut(s)	552
BmsI	GCATC	3 cut(s)	222, 841, 1347
BmtI	GCTAGC	1 cut(s)	416
BpiI	GAAGAC	4 cut(s)	373, 849, 900, 1383
BpmI	CTGGAG	2 cut(s)	231, 579
Bpu1102I	GCTNAGC	1 cut(s)	429
Bpu14I	TTCGAA	3 cut(s)	180, 840, 1374
BsaHI	GRCGYC	1 cut(s)	398
BsaI	GGTCTC	2 cut(s)	1217, 1330
BsaJI	CCNNGG	2 cut(s)	1090, 1148
BsaWI	WCCGGW	1 cut(s)	1392
BsaXI	ACNNNNNCTCC	2 cut(s)	137, 167
Bsc4I	CCNNNNNNNGG	2 cut(s)	798, 1096
Bse1I	ACTGG	4 cut(s)	248, 316, 596, 1206
Bse3DI	GCAATG	1 cut(s)	1353
BseBI	CCWGG	1 cut(s)	552
BseDI	CCNNGG	2 cut(s)	1090, 1148
BseGI	GGATG	3 cut(s)	164, 237, 876
BseLI	CCNNNNNNNGG	2 cut(s)	798, 1096
BseMI	GCAATG	1 cut(s)	1353
BseMII	CTCAG	5 cut(s)	189, 320, 420, 1025, 1212
BseNI	ACTGG	4 cut(s)	248, 316, 596, 1206
BseRI	GAGGAG	3 cut(s)	35, 108, 1209
BseSI	GKGCMC	1 cut(s)	876
BseXI	GCAGC	2 cut(s)	316, 838
BsgI	GTGCAG	1 cut(s)	1014
Bsh1236I	CGCG	1 cut(s)	386
BsiHKAI	GWGCWC	1 cut(s)	876
BsiSI	CCGG	3 cut(s)	17, 282, 1393
BslFI	GGGAC	1 cut(s)	73
BslI	CCNNNNNNNGG	2 cut(s)	798, 1096
BsmAI	GTCTC	4 cut(s)	282, 931, 1217, 1330
BsmBI	CGTCTC	1 cut(s)	282
BsmFI	GGGAC	1 cut(s)	73
BsmI	GAATGC	2 cut(s)	903, 997
Bso31I	GGTCTC	2 cut(s)	1217, 1330
Bsp119I	TTCGAA	3 cut(s)	180, 840, 1374
Bsp1286I	GDGCHC	2 cut(s)	189, 876
Bsp143I	GATC	3 cut(s)	367, 908, 1144
Bsp1720I	GCTNAGC	1 cut(s)	429
BspACI	CCGC	5 cut(s)	55, 168, 355, 635, 1421
BspCNI	CTCAG	5 cut(s)	190, 319, 421, 1024, 1213
BspFNI	CGCG	1 cut(s)	386
BspLI	GGNNCC	2 cut(s)	9, 1278
BspOI	GCTAGC	1 cut(s)	416
BspPI	GGATC	2 cut(s)	362, 1139
BspT104I	TTCGAA	3 cut(s)	180, 840, 1374
BspTI	CTTAAG	1 cut(s)	1436
BspTNI	GGTCTC	2 cut(s)	1217, 1330
BsrDI	GCAATG	1 cut(s)	1353
BsrI	ACTGG	4 cut(s)	248, 316, 596, 1206
BssECI	CCNNGG	2 cut(s)	1090, 1148
BssMI	GATC	3 cut(s)	367, 908, 1144
BssNI	GRCGYC	1 cut(s)	398
BssT1I	CCWWGG	1 cut(s)	1148
Bst2UI	CCWGG	1 cut(s)	552
Bst4CI	ACNGT	4 cut(s)	73, 811, 1111, 1307
Bst6I	CTCTTC	1 cut(s)	1248
BstACI	GRCGYC	1 cut(s)	398
BstAFI	CTTAAG	1 cut(s)	1436
BstBI	TTCGAA	3 cut(s)	180, 840, 1374
BstC8I	GCNNGC	6 cut(s)	266, 303, 414, 426, 1071, 1087
BstDEI	CTNAG	7 cut(s)	198, 306, 429, 619, 1011, 1034, 1221
BstDSI	CCRYGG	1 cut(s)	1090
BstF5I	GGATG	3 cut(s)	164, 237, 876
BstFNI	CGCG	1 cut(s)	386
BstHHI	GCGC	1 cut(s)	388
BstKTI	GATC	3 cut(s)	370, 911, 1147
BstMAI	GTCTC	4 cut(s)	282, 931, 1217, 1330
BstMBI	GATC	3 cut(s)	367, 908, 1144
BstMWI	GCNNNNNNNGC	5 cut(s)	203, 232, 335, 430, 970
BstNI	CCWGG	1 cut(s)	552
BstNSI	RCATGY	1 cut(s)	945
BstSCI	CCNGG	1 cut(s)	550
BstSLI	GKGCMC	1 cut(s)	876
BstUI	CGCG	1 cut(s)	386
BstV1I	GCAGC	2 cut(s)	316, 838
BstV2I	GAAGAC	4 cut(s)	373, 849, 900, 1383
BstX2I	RGATCY	2 cut(s)	367, 1144
BstXI	CCANNNNNNTGG	1 cut(s)	1342
BstYI	RGATCY	2 cut(s)	367, 1144
BtgI	CCRYGG	1 cut(s)	1090
BtsCI	GGATG	3 cut(s)	164, 237, 876
BtsI	GCAGTG	2 cut(s)	213, 259
BtsIMutI	CAGTG	5 cut(s)	213, 259, 692, 1218, 1299
Cac8I	GCNNGC	6 cut(s)	266, 303, 414, 426, 1071, 1087
CaiI	CAGNNNCTG	1 cut(s)	691
CfoI	GCGC	1 cut(s)	388
Cfr13I	GGNCC	1 cut(s)	106
CseI	GACGC	2 cut(s)	406, 511
Csp6I	GTAC	2 cut(s)	1058, 1176
CviAII	CATG	4 cut(s)	43, 730, 916, 942
CviQI	GTAC	2 cut(s)	1058, 1176
DdeI	CTNAG	7 cut(s)	198, 306, 429, 619, 1011, 1034, 1221
DpnI	GATC	3 cut(s)	369, 910, 1146
DpnII	GATC	3 cut(s)	367, 908, 1144
DraIII	CACNNNGTG	1 cut(s)	265
Eam1104I	CTCTTC	1 cut(s)	1248
EarI	CTCTTC	1 cut(s)	1248
EciI	GGCGGA	2 cut(s)	157, 650
Eco130I	CCWWGG	1 cut(s)	1148
Eco24I	GRGCYC	1 cut(s)	189
Eco31I	GGTCTC	2 cut(s)	1217, 1330
Eco47I	GGWCC	1 cut(s)	106
Eco57I	CTGAAG	1 cut(s)	384
EcoRI	GAATTC	1 cut(s)	1006
EcoRII	CCWGG	1 cut(s)	550
EcoT14I	CCWWGG	1 cut(s)	1148
EcoT22I	ATGCAT	1 cut(s)	999
EcoT38I	GRGCYC	1 cut(s)	189
ErhI	CCWWGG	1 cut(s)	1148
Esp3I	CGTCTC	1 cut(s)	282
FaeI	CATG	4 cut(s)	46, 733, 919, 945
FalI	AAGNNNNNCTT	2 cut(s)	1419, 1451
FaqI	GGGAC	1 cut(s)	73
FatI	CATG	4 cut(s)	42, 729, 915, 941
FauI	CCCGC	1 cut(s)	62
FblI	GTMKAC	1 cut(s)	703
Fnu4HI	GCNGC	2 cut(s)	330, 827
FokI	GGATG	3 cut(s)	151, 244, 863
FriOI	GRGCYC	1 cut(s)	189
Fsp4HI	GCNGC	2 cut(s)	330, 827
FspBI	CTAG	8 cut(s)	150, 405, 413, 606, 735, 1149, 1182, 1361
GlaI	GCGC	1 cut(s)	387
GluI	GCNGC	2 cut(s)	330, 827
GsuI	CTGGAG	2 cut(s)	231, 579
HapII	CCGG	3 cut(s)	17, 282, 1393
HgaI	GACGC	2 cut(s)	406, 511
HhaI	GCGC	1 cut(s)	388
Hin1I	GRCGYC	1 cut(s)	398
Hin1II	CATG	4 cut(s)	46, 733, 919, 945
Hin6I	GCGC	1 cut(s)	386
HinP1I	GCGC	1 cut(s)	386
HincII	GTYRAC	3 cut(s)	501, 814, 1114
HindII	GTYRAC	3 cut(s)	501, 814, 1114
HindIII	AAGCTT	1 cut(s)	774
HinfI	GANTC	5 cut(s)	33, 101, 378, 558, 1371
HpaII	CCGG	3 cut(s)	17, 282, 1393
HphI	GGTGA	2 cut(s)	28, 344
Hpy166II	GTNNAC	7 cut(s)	79, 239, 501, 704, 814, 874, 1114
Hpy188I	TCNGA	6 cut(s)	106, 157, 191, 309, 1005, 1140
Hpy188III	TCNNGA	4 cut(s)	525, 584, 606, 647
Hpy8I	GTNNAC	7 cut(s)	79, 239, 501, 704, 814, 874, 1114
HpyAV	CCTTC	3 cut(s)	20, 176, 791
HpyCH4III	ACNGT	4 cut(s)	73, 811, 1111, 1307
HpyCH4IV	ACGT	3 cut(s)	215, 291, 1174
HpyCH4V	TGCA	4 cut(s)	874, 936, 997, 1031
HpyF10VI	GCNNNNNNNGC	5 cut(s)	203, 232, 335, 430, 970
HpyF3I	CTNAG	7 cut(s)	198, 306, 429, 619, 1011, 1034, 1221
HpySE526I	ACGT	3 cut(s)	215, 291, 1174
Hsp92I	GRCGYC	1 cut(s)	398
Hsp92II	CATG	4 cut(s)	46, 733, 919, 945
HspAI	GCGC	1 cut(s)	386
Kzo9I	GATC	3 cut(s)	367, 908, 1144
LmnI	GCTCC	1 cut(s)	158
Lsp1109I	GCAGC	2 cut(s)	316, 838
LweI	GCATC	3 cut(s)	222, 841, 1347
MaeI	CTAG	8 cut(s)	150, 405, 413, 606, 735, 1149, 1182, 1361
MaeII	ACGT	3 cut(s)	215, 291, 1174
MaeIII	GTNAC	4 cut(s)	394, 669, 737, 1315
MalI	GATC	3 cut(s)	369, 910, 1146
MboI	GATC	3 cut(s)	367, 908, 1144
MflI	RGATCY	2 cut(s)	367, 1144
MhlI	GDGCHC	2 cut(s)	189, 876
MlyI	GAGTC	1 cut(s)	387
MmeI	TCCRAC	3 cut(s)	180, 1150, 1243
Mph1103I	ATGCAT	1 cut(s)	999
MseI	TTAA	5 cut(s)	438, 803, 953, 1386, 1437
MslI	CAYNNNNRTG	1 cut(s)	41
MspCI	CTTAAG	1 cut(s)	1436
MspI	CCGG	3 cut(s)	17, 282, 1393
MspR9I	CCNGG	1 cut(s)	552
Mva1269I	GAATGC	2 cut(s)	903, 997
MvaI	CCWGG	1 cut(s)	552
MvnI	CGCG	1 cut(s)	386
MwoI	GCNNNNNNNGC	5 cut(s)	203, 232, 335, 430, 970
NdeII	GATC	3 cut(s)	367, 908, 1144
NheI	GCTAGC	1 cut(s)	412
NlaIII	CATG	4 cut(s)	46, 733, 919, 945
NlaIV	GGNNCC	2 cut(s)	9, 1278
NmuCI	GTSAC	2 cut(s)	394, 1315
NsiI	ATGCAT	1 cut(s)	999
NspI	RCATGY	1 cut(s)	945
NspV	TTCGAA	3 cut(s)	180, 840, 1374
PciI	ACATGT	1 cut(s)	941
PcsI	WCGNNNNNNNCGW	1 cut(s)	1171
PctI	GAATGC	2 cut(s)	903, 997
PfeI	GAWTC	4 cut(s)	33, 101, 558, 1371
PflFI	GACNNNGTC	1 cut(s)	1118
PfoI	TCCNGGA	1 cut(s)	550
PkrI	GCNGC	2 cut(s)	331, 828
PleI	GAGTC	1 cut(s)	386
PpsI	GAGTC	1 cut(s)	386
PscI	ACATGT	1 cut(s)	941
Psp6I	CCWGG	1 cut(s)	550
PspGI	CCWGG	1 cut(s)	550
PspN4I	GGNNCC	2 cut(s)	9, 1278
PspPI	GGNCC	1 cut(s)	106
PstNI	CAGNNNCTG	1 cut(s)	691
PsuI	RGATCY	2 cut(s)	367, 1144
PsyI	GACNNNGTC	1 cut(s)	1118
RsaI	GTAC	2 cut(s)	1059, 1177
RsaNI	GTAC	2 cut(s)	1058, 1176
RseI	CAYNNNNRTG	1 cut(s)	41
SaqAI	TTAA	5 cut(s)	438, 803, 953, 1386, 1437
SatI	GCNGC	2 cut(s)	330, 827
Sau3AI	GATC	3 cut(s)	367, 908, 1144
Sau96I	GGNCC	1 cut(s)	106
SchI	GAGTC	1 cut(s)	387
ScrFI	CCNGG	1 cut(s)	552
SduI	GDGCHC	2 cut(s)	189, 876
SfaNI	GCATC	3 cut(s)	222, 841, 1347
SfuI	TTCGAA	3 cut(s)	180, 840, 1374
SinI	GGWCC	1 cut(s)	106
SmiMI	CAYNNNNRTG	1 cut(s)	41
SmlI	CTYRAG	1 cut(s)	1436
SmoI	CTYRAG	1 cut(s)	1436
SpeI	ACTAGT	1 cut(s)	734
SsiI	CCGC	5 cut(s)	55, 168, 355, 635, 1421
SspMI	CTAG	8 cut(s)	150, 405, 413, 606, 735, 1149, 1182, 1361
StyD4I	CCNGG	1 cut(s)	550
StyI	CCWWGG	1 cut(s)	1148
TaaI	ACNGT	4 cut(s)	73, 811, 1111, 1307
TaiI	ACGT	3 cut(s)	218, 294, 1177
TaqI	TCGA	4 cut(s)	180, 840, 907, 1374
TaqII	GACCGA	1 cut(s)	1241
TfiI	GAWTC	4 cut(s)	33, 101, 558, 1371
Tru1I	TTAA	5 cut(s)	438, 803, 953, 1386, 1437
Tru9I	TTAA	5 cut(s)	438, 803, 953, 1386, 1437
TscAI	CASTG	5 cut(s)	213, 266, 692, 1225, 1306
TseFI	GTSAC	2 cut(s)	394, 1315
TseI	GCWGC	2 cut(s)	329, 826
Tsp45I	GTSAC	2 cut(s)	394, 1315
TspDTI	ATGAA	5 cut(s)	801, 810, 823, 1151, 1367
TspGWI	ACGGA	1 cut(s)	1423
TspRI	CASTG	5 cut(s)	213, 266, 692, 1225, 1306
Tth111I	GACNNNGTC	1 cut(s)	1118
Vha464I	CTTAAG	1 cut(s)	1436
VneI	GTGCAC	1 cut(s)	872
VpaK11BI	GGWCC	1 cut(s)	106
XapI	RAATTY	4 cut(s)	131, 460, 1006, 1040
XbaI	TCTAGA	1 cut(s)	605
XceI	RCATGY	1 cut(s)	945
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1093
XmaJI	CCTAGG	1 cut(s)	1148
XmiI	GTMKAC	1 cut(s)	703
XspI	CTAG	8 cut(s)	150, 405, 413, 606, 735, 1149, 1182, 1361
Zsp2I	ATGCAT	1 cut(s)	999

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.