Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0010462

Physical Location & Seq

Genomic Coordinates Forward (+)

76294259 .. 76298161

Gene Structure 3903 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00054920.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1920 bp

ATGGACGGAGAAGAAGAGACCACTGTATCTATAGACTTCCCTCTCTTCAAACCCAGAGCATCTAAACCCGACCCGGATCCGATAACCCATGCCGCCGCCGCCGCCGCCGAACCGGACCTTGAGAGGTCTGCAGACCCGGACGCGAACTCCGACGCCAAGTTCACCGACCTCGGCCTCGCCGACTGGCTGCTCCGGACGCTGGCCGAGCTCGGTCTGAAGAAGCCGACGCCGGTCCAGGCCAACTGCATCCCCAAAATCCTGGAGGGCCGGGACGTGCTGGGCCTGGCCCAGACCGGTAGCGGCAAGACGGCGGCGTTCGCGCTGCCGATTCTGCAGCGGCTGGCGGAGGGGCCTTATGGGGTTTTCGCGCTGGTGATGACGCCGACGCATGAGCTGGCGTACCAGCTCGCCGAGCAGTTCAGGGCTTTCGGGTCGTCGCTGCATTTGAGGTGCTCGGTGATTGTTGGAGGTATGGATAAGCTGAAGCAAGCTCAGAGCTTGATGGGTAGGCCCCATGTGGTGATTGCCACTCCCGGTAGGGTTAGGGATTTGCTTCAGGAGGACCCTGATTTTCCCTCTGTTTTCTCCAAGGCCAAGTTCCTAGTGCTGGATGAGGCAGATAGGTTATTGAATGCTGAATTTGAGGCTGATTTGAGAGTGGTGTTTCAGTGCTTGCCGAAGAATCGGCAGACCCTGTTGTTTTCTGCAACAATGACTAGTGACCTGCAAGCACTGATTGAAGTTTCTGCAAATAAGGCGTACTTTTACCAGGCTTATGAAGGTCTCAGGACGGTTGGCACTCTTGCGCAGAAATATGTTTTCATACCCCGTGAAGCGAAAGAAGTTTATTTGGTGTATATTTTGTCGAAAATGAAAGATATGGGTATTCGTTCAGCCATCGTGTTTGTCTCAACCCTATGGAATTGTCACTTGTTGAATTTTTTACTGGAGGAGCTTGGTCAAAGTGTTTCAGCGTTGCACTCATCCAAATCCCAGCCTCTTAGACTTTCTGCATTACATAAGTTCAAATCTGGACAGGTTCCAGTGTTGATTGCAACTGATCTTGCCAATCGTGGTTTGGATATTCCTACGGTTGACCTAGTAATCAATTATGATATTCCAAGTGATCCAAAGGATTATGTTCACCGTGTGGGACGTACTGCAAGAGCAGGCAGAGGAGGTTTAGCTGTCAGCGTTGTTACCCAACTTGATGTAAAGCTGCTTCATAAAATAGAAGAAGAAGTTGGGGAACAGTTGGTAGAATTTGAATGCAAGGAGAACGAGGTTCTTGAGGATATCCGTAAGGTTTACACAGCCAGGCGTGTCGCAAAGATGAAGATGATGGAATCCAACTTTGTAGAAAGAGAGAAAAAACGGAAAGAACGAACACTAAAGACGCTAGAAGCGAAAGAGGAGGACACCATTCAAGAAAAGAAGACGATCTCCCTGAAGACCTCCGACGATGAGATCTTCGAATTGGAGGAGGACGTAGCGATGGAGTTCGGAACCGTCAAGGCCTTCTTTGGAGACGATGGGGTCTCCCGAGACATGGTGATGCCTATACCTAACGTGCACAGCAAGGAACTGACGAGGATCATTGACTTCTGCACCAAGCATCTCGCCTTGAAGCCGAAAGCGGAGCACGACGAGGCCGGGAAGAAGGAGCTGAGGAAGTTCTATGCTGAGTACGTGAAGGAGGACACCACAGAACGCATAATGGAGCTCATTCTGGCAGCTGATTATTTGAATGTGAATGACTTGCTGGACTTGTTGAACCAGTCCGTGGCGGACCGGATCAAGAACAAGAGTGTGGAGTATGTAAGGAAGTTGTTTGGGATTGAGAGCGATTTTACTCCCGAGGAGGAGGCAGCGCTCCGGGAGGAGTATGCCTGGGCTTTTGAGGGGGTTGATGAAGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0007275 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009553 GO:0009561 GO:0009791 GO:0009987 GO:0010467 GO:0010501 GO:0016070 GO:0016072 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022613 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0042623 GO:0042995 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0048229 GO:0048856 GO:0070013 GO:0070035 GO:0071704 GO:0071840 GO:0090304 GO:0090406 GO:0120025 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

639

Amino Acids

71.72

Weight (kDa)

5.21

Isoelectric Point (pI)

34.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ResIII	PF04851	74 - 238	3.1e-06	Type III restriction enzyme, res subunit
DEAD	PF00270	76 - 244	1.5e-47	DEAD/DEAH box helicase
Helicase_C	PF00271	293 - 391	2e-26	Helicase conserved C-terminal domain
Skp1_POZ	PF03931	480 - 540	6.4e-13	Skp1 family, tetramerisation domain
Skp1	PF01466	586 - 632	4.3e-20	Skp1 family, dimerisation domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0010462)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G16280
fragaria_vesca	FvH4_3g14750 FvH4_3g14750 FvH4_3g14750
malus_domestica	MD10G1209000.v1.1
prunus_persica	Prupe.4G132500_v2.0.a1
pyrus_communis	pycom10g17780 pycom13g14880
rosa_chinensis	RchiOBHm_Chr5g0024261
rosa_laevigata	RLG00000032788
rosa_multiflora	Rmu_sc0002344.1_g000004 Rmu_sc0018255.1_g000002
rosa_roxburghii	Rroxscaffold_1G00054920 Rroxscaffold_1G00055760
rosa_rugosa	Rorug05G0082900
rosa_samantha	Rh5AG173300 Rh5BG170200 Rh5CG186300 Rh5DG172800
rosa_wichuraiana	Rw0G000850 Rw5G015650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1535
Acc16I	TGCGCA	1 cut(s)	807
Acc36I	ACCTGC	1 cut(s)	732
AccB7I	CCANNNNNTGG	1 cut(s)	1783
AccII	CGCG	3 cut(s)	143, 320, 368
AccIII	TCCGGA	1 cut(s)	192
AclWI	GGATC	5 cut(s)	71, 84, 1121, 1601, 1802
AcoI	YGGCCR	1 cut(s)	201
AcsI	RAATTY	3 cut(s)	638, 937, 1262
AcuI	CTGAAG	4 cut(s)	236, 503, 539, 1469
AcyI	GRCGYC	3 cut(s)	153, 227, 380
AdeI	CACNNNGTG	1 cut(s)	1150
AfaI	GTAC	4 cut(s)	401, 761, 1159, 1688
AfeI	AGCGCT	1 cut(s)	1872
AfiI	CCNNNNNNNGG	4 cut(s)	199, 607, 1549, 1783
AgeI	ACCGGT	1 cut(s)	293
AhlI	ACTAGT	1 cut(s)	716
AjiI	CACGTC	1 cut(s)	274
AjnI	CCWGG	6 cut(s)	234, 258, 282, 768, 1316, 1889
AjuI	GAANNNNNNNTTGG	4 cut(s)	581, 613, 1227, 1259
Alw21I	GWGCWC	5 cut(s)	210, 455, 1575, 1644, 1725
Alw26I	GTCTC	6 cut(s)	11, 788, 913, 1521, 1539, 1543
Alw44I	GTGCAC	1 cut(s)	1571
AlwI	GGATC	5 cut(s)	71, 84, 1121, 1601, 1802
AlwNI	CAGNNNCTG	2 cut(s)	340, 694
Ama87I	CYCGRG	2 cut(s)	1542, 1856
Aor13HI	TCCGGA	1 cut(s)	192
Aor51HI	AGCGCT	1 cut(s)	1872
ApaLI	GTGCAC	1 cut(s)	1571
ApeKI	GCWGC	7 cut(s)	187, 322, 334, 439, 1219, 1733, 1868
ApoI	RAATTY	3 cut(s)	638, 937, 1262
AsiGI	ACCGGT	1 cut(s)	293
AspLEI	GCGC	4 cut(s)	322, 370, 808, 1873
AspS9I	GGNCC	9 cut(s)	115, 232, 265, 280, 286, 350, 510, 562, 1789
AsuC2I	CCSGG	6 cut(s)	74, 137, 269, 534, 1654, 1877
AsuHPI	GGTGA	6 cut(s)	154, 385, 469, 532, 1136, 1564
AsuII	TTCGAA	1 cut(s)	1473
AvaI	CYCGRG	2 cut(s)	1542, 1856
AvaII	GGWCC	4 cut(s)	115, 232, 562, 1789
BaeGI	GKGCMC	1 cut(s)	1575
BaeI	ACNNNNGTAYC	2 cut(s)	9, 42
BamHI	GGATCC	1 cut(s)	76
BanII	GRGCYC	2 cut(s)	210, 1725
BarI	GAAGNNNNNNTAC	2 cut(s)	1206, 1238
BbsI	GAAGAC	2 cut(s)	1442, 1457
Bbv12I	GWGCWC	5 cut(s)	210, 455, 1575, 1644, 1725
BbvCI	CCTCAGC	1 cut(s)	1667
BbvI	GCAGC	7 cut(s)	174, 309, 346, 426, 1206, 1745, 1880
BccI	CCATC	5 cut(s)	496, 905, 1336, 1489, 1526
BceAI	ACGGC	1 cut(s)	324
BciT130I	CCWGG	6 cut(s)	236, 260, 284, 770, 1318, 1891
BcnI	CCSGG	6 cut(s)	74, 137, 269, 534, 1654, 1877
BcoDI	GTCTC	6 cut(s)	11, 788, 913, 1521, 1539, 1543
BcuI	ACTAGT	1 cut(s)	716
BfaI	CTAG	4 cut(s)	602, 717, 1100, 1400
BfmI	CTRYAG	3 cut(s)	30, 129, 332
BfoI	RGCGCY	1 cut(s)	1874
BfuAI	ACCTGC	1 cut(s)	732
BglII	AGATCT	1 cut(s)	1467
Bme18I	GGWCC	4 cut(s)	115, 232, 562, 1789
BmeT110I	CYCGRG	2 cut(s)	1542, 1856
BmgBI	CACGTC	1 cut(s)	274
BmgT120I	GGNCC	9 cut(s)	115, 232, 265, 280, 286, 350, 510, 562, 1789
BmiI	GGNNCC	6 cut(s)	78, 351, 512, 564, 1041, 1507
BmsI	GCATC	4 cut(s)	68, 255, 1545, 1624
BoxI	GACNNNNGTC	1 cut(s)	230
BpiI	GAAGAC	2 cut(s)	1442, 1457
BpmI	CTGGAG	2 cut(s)	281, 968
Bpu10I	CCTNAGC	1 cut(s)	1667
Bpu14I	TTCGAA	1 cut(s)	1473
BpuEI	CTTGAG	2 cut(s)	140, 1310
BpuMI	CCSGG	6 cut(s)	74, 137, 269, 534, 1654, 1877
BsaAI	YACGTR	1 cut(s)	1690
BsaHI	GRCGYC	3 cut(s)	153, 227, 380
BsaI	GGTCTC	3 cut(s)	11, 788, 1543
BsaJI	CCNNGG	5 cut(s)	169, 588, 1782, 1857, 1890
BsaWI	WCCGGW	4 cut(s)	112, 192, 293, 1791
BsaXI	ACNNNNNCTCC	2 cut(s)	131, 161
Bsc4I	CCNNNNNNNGG	4 cut(s)	199, 607, 1549, 1783
Bse118I	RCCGGY	2 cut(s)	229, 293
Bse1I	ACTGG	4 cut(s)	188, 951, 1043, 1777
BseAI	TCCGGA	1 cut(s)	192
BseBI	CCWGG	6 cut(s)	236, 260, 284, 770, 1318, 1891
BseDI	CCNNGG	5 cut(s)	169, 588, 1782, 1857, 1890
BseGI	GGATG	3 cut(s)	246, 616, 983
BseLI	CCNNNNNNNGG	4 cut(s)	199, 607, 1549, 1783
BseMII	CTCAG	4 cut(s)	506, 799, 1658, 1674
BseNI	ACTGG	4 cut(s)	188, 951, 1043, 1777
BseRI	GAGGAG	7 cut(s)	965, 1191, 1427, 1496, 1874, 1877, 1895
BseSI	GKGCMC	1 cut(s)	1575
BseXI	GCAGC	7 cut(s)	174, 309, 346, 426, 1206, 1745, 1880
BseYI	CCCAGC	2 cut(s)	277, 993
BsgI	GTGCAG	1 cut(s)	1591
Bsh1236I	CGCG	3 cut(s)	143, 320, 368
BshTI	ACCGGT	1 cut(s)	293
BsiHKAI	GWGCWC	5 cut(s)	210, 455, 1575, 1644, 1725
BsiHKCI	CYCGRG	2 cut(s)	1542, 1856
BslFI	GGGAC	2 cut(s)	284, 1167
BslI	CCNNNNNNNGG	4 cut(s)	199, 607, 1549, 1783
BsmAI	GTCTC	6 cut(s)	11, 788, 913, 1521, 1539, 1543
BsmBI	CGTCTC	1 cut(s)	1521
BsmFI	GGGAC	2 cut(s)	284, 1167
BsmI	GAATGC	2 cut(s)	637, 1274
Bso31I	GGTCTC	3 cut(s)	11, 788, 1543
BsoBI	CYCGRG	2 cut(s)	1542, 1856
Bsp119I	TTCGAA	1 cut(s)	1473
Bsp1286I	GDGCHC	5 cut(s)	210, 455, 1575, 1644, 1725
Bsp13I	TCCGGA	1 cut(s)	192
Bsp143I	GATC	7 cut(s)	76, 1060, 1126, 1440, 1467, 1593, 1794
BspCNI	CTCAG	4 cut(s)	505, 798, 1659, 1675
BspEI	TCCGGA	1 cut(s)	192
BspFNI	CGCG	3 cut(s)	143, 320, 368
BspLI	GGNNCC	6 cut(s)	78, 351, 512, 564, 1041, 1507
BspMAI	CTGCAG	2 cut(s)	133, 336
BspMI	ACCTGC	1 cut(s)	732
BspPI	GGATC	5 cut(s)	71, 84, 1121, 1601, 1802
BspT104I	TTCGAA	1 cut(s)	1473
BspTNI	GGTCTC	3 cut(s)	11, 788, 1543
BsrFI	RCCGGY	2 cut(s)	229, 293
BsrI	ACTGG	4 cut(s)	188, 951, 1043, 1777
BssAI	RCCGGY	2 cut(s)	229, 293
BssECI	CCNNGG	5 cut(s)	169, 588, 1782, 1857, 1890
BssMI	GATC	7 cut(s)	76, 1060, 1126, 1440, 1467, 1593, 1794
BssNI	GRCGYC	3 cut(s)	153, 227, 380
BssT1I	CCWWGG	1 cut(s)	588
Bst2UI	CCWGG	6 cut(s)	236, 260, 284, 770, 1318, 1891
Bst4CI	ACNGT	6 cut(s)	25, 793, 1093, 1148, 1254, 1510
Bst6I	CTCTTC	2 cut(s)	9, 50
BstACI	GRCGYC	3 cut(s)	153, 227, 380
BstBAI	YACGTR	1 cut(s)	1690
BstBI	TTCGAA	1 cut(s)	1473
BstC8I	GCNNGC	8 cut(s)	201, 342, 396, 408, 489, 674, 729, 1171
BstDEI	CTNAG	5 cut(s)	492, 785, 1001, 1667, 1683
BstDSI	CCRYGG	1 cut(s)	1782
BstF5I	GGATG	3 cut(s)	246, 616, 983
BstFNI	CGCG	3 cut(s)	143, 320, 368
BstH2I	RGCGCY	1 cut(s)	1874
BstHHI	GCGC	4 cut(s)	322, 370, 808, 1873
BstKTI	GATC	7 cut(s)	79, 1063, 1129, 1443, 1470, 1596, 1797
BstMAI	GTCTC	6 cut(s)	11, 788, 913, 1521, 1539, 1543
BstMBI	GATC	7 cut(s)	76, 1060, 1126, 1440, 1467, 1593, 1794
BstMWI	GCNNNNNNNGC	9 cut(s)	98, 101, 104, 196, 205, 317, 331, 412, 755
BstNI	CCWGG	6 cut(s)	236, 260, 284, 770, 1318, 1891
BstPAI	GACNNNNGTC	1 cut(s)	230
BstSFI	CTRYAG	3 cut(s)	30, 129, 332
BstSLI	GKGCMC	1 cut(s)	1575
BstUI	CGCG	3 cut(s)	143, 320, 368
BstV1I	GCAGC	7 cut(s)	174, 309, 346, 426, 1206, 1745, 1880
BstV2I	GAAGAC	2 cut(s)	1442, 1457
BstX2I	RGATCY	2 cut(s)	76, 1467
BstXI	CCANNNNNNTGG	1 cut(s)	259
BstYI	RGATCY	2 cut(s)	76, 1467
BtgI	CCRYGG	1 cut(s)	1782
BtgZI	GCGATG	1 cut(s)	1508
BtrI	CACGTC	1 cut(s)	274
BtsCI	GGATG	3 cut(s)	246, 616, 983
BtsIMutI	CAGTG	4 cut(s)	21, 674, 731, 1050
BveI	ACCTGC	1 cut(s)	732
Cac8I	GCNNGC	8 cut(s)	201, 342, 396, 408, 489, 674, 729, 1171
CaiI	CAGNNNCTG	2 cut(s)	340, 694
CfoI	GCGC	4 cut(s)	322, 370, 808, 1873
Cfr10I	RCCGGY	2 cut(s)	229, 293
Cfr13I	GGNCC	9 cut(s)	115, 232, 265, 280, 286, 350, 510, 562, 1789
CpoI	CGGWCCG	1 cut(s)	1789
CseI	GACGC	7 cut(s)	149, 161, 205, 235, 388, 394, 1405
Csp6I	GTAC	4 cut(s)	400, 760, 1158, 1687
CspAI	ACCGGT	1 cut(s)	293
CspI	CGGWCCG	1 cut(s)	1789
CviAII	CATG	4 cut(s)	89, 389, 515, 1549
CviQI	GTAC	4 cut(s)	400, 760, 1158, 1687
DdeI	CTNAG	5 cut(s)	492, 785, 1001, 1667, 1683
DpnI	GATC	7 cut(s)	78, 1062, 1128, 1442, 1469, 1595, 1796
DpnII	GATC	7 cut(s)	76, 1060, 1126, 1440, 1467, 1593, 1794
DraIII	CACNNNGTG	1 cut(s)	1150
DrdI	GACNNNNNNGTC	1 cut(s)	1535
DseDI	GACNNNNNNGTC	1 cut(s)	1535
EaeI	YGGCCR	1 cut(s)	201
Eam1104I	CTCTTC	2 cut(s)	9, 50
EarI	CTCTTC	2 cut(s)	9, 50
EciI	GGCGGA	2 cut(s)	359, 1802
Ecl136II	GAGCTC	2 cut(s)	208, 1723
Eco130I	CCWWGG	1 cut(s)	588
Eco147I	AGGCCT	1 cut(s)	1517
Eco24I	GRGCYC	2 cut(s)	210, 1725
Eco31I	GGTCTC	3 cut(s)	11, 788, 1543
Eco32I	GATATC	1 cut(s)	1297
Eco47I	GGWCC	4 cut(s)	115, 232, 562, 1789
Eco47III	AGCGCT	1 cut(s)	1872
Eco53kI	GAGCTC	2 cut(s)	208, 1723
Eco57I	CTGAAG	4 cut(s)	236, 503, 539, 1469
Eco88I	CYCGRG	2 cut(s)	1542, 1856
EcoICRI	GAGCTC	2 cut(s)	208, 1723
EcoO109I	RGGNCCY	3 cut(s)	350, 510, 562
EcoRII	CCWGG	6 cut(s)	234, 258, 282, 768, 1316, 1889
EcoRV	GATATC	1 cut(s)	1297
EcoT14I	CCWWGG	1 cut(s)	588
EcoT38I	GRGCYC	2 cut(s)	210, 1725
ErhI	CCWWGG	1 cut(s)	588
Esp3I	CGTCTC	1 cut(s)	1521
FaeI	CATG	4 cut(s)	92, 392, 518, 1552
FalI	AAGNNNNNCTT	4 cut(s)	746, 778, 1505, 1537
FaqI	GGGAC	2 cut(s)	284, 1167
FatI	CATG	4 cut(s)	88, 388, 514, 1548
FokI	GGATG	3 cut(s)	233, 623, 970
FriOI	GRGCYC	2 cut(s)	210, 1725
FspBI	CTAG	4 cut(s)	602, 717, 1100, 1400
FspI	TGCGCA	1 cut(s)	807
GlaI	GCGC	4 cut(s)	321, 369, 807, 1872
GsaI	CCCAGC	2 cut(s)	281, 997
GsuI	CTGGAG	2 cut(s)	281, 968
HaeII	RGCGCY	1 cut(s)	1874
HgaI	GACGC	7 cut(s)	149, 161, 205, 235, 388, 394, 1405
HhaI	GCGC	4 cut(s)	322, 370, 808, 1873
Hin1I	GRCGYC	3 cut(s)	153, 227, 380
Hin1II	CATG	4 cut(s)	92, 392, 518, 1552
Hin6I	GCGC	4 cut(s)	320, 368, 806, 1871
HinP1I	GCGC	4 cut(s)	320, 368, 806, 1871
HincII	GTYRAC	1 cut(s)	1096
HindII	GTYRAC	1 cut(s)	1096
HinfI	GANTC	3 cut(s)	328, 682, 1346
HphI	GGTGA	6 cut(s)	154, 385, 469, 532, 1136, 1564
Hpy166II	GTNNAC	5 cut(s)	162, 1096, 1144, 1309, 1573
Hpy188I	TCNGA	6 cut(s)	81, 151, 216, 495, 1459, 1505
Hpy188III	TCNNGA	9 cut(s)	193, 557, 787, 1032, 1289, 1427, 1542, 1798, 1856
Hpy8I	GTNNAC	5 cut(s)	162, 1096, 1144, 1309, 1573
Hpy99I	CGWCG	6 cut(s)	155, 229, 388, 439, 1463, 1649
HpyAV	CCTTC	4 cut(s)	773, 1528, 1654, 1687
HpyCH4III	ACNGT	6 cut(s)	25, 793, 1093, 1148, 1254, 1510
HpyCH4IV	ACGT	5 cut(s)	273, 1156, 1488, 1569, 1689
HpyF10VI	GCNNNNNNNGC	9 cut(s)	98, 101, 104, 196, 205, 317, 331, 412, 755
HpyF3I	CTNAG	5 cut(s)	492, 785, 1001, 1667, 1683
HpySE526I	ACGT	5 cut(s)	273, 1156, 1488, 1569, 1689
Hsp92I	GRCGYC	3 cut(s)	153, 227, 380
Hsp92II	CATG	4 cut(s)	92, 392, 518, 1552
HspAI	GCGC	4 cut(s)	320, 368, 806, 1871
Kpn2I	TCCGGA	1 cut(s)	192
Kzo9I	GATC	7 cut(s)	76, 1060, 1126, 1440, 1467, 1593, 1794
LmnI	GCTCC	6 cut(s)	195, 952, 1639, 1663, 1720, 1878
Lsp1109I	GCAGC	7 cut(s)	174, 309, 346, 426, 1206, 1745, 1880
LweI	GCATC	4 cut(s)	68, 255, 1545, 1624
MaeI	CTAG	4 cut(s)	602, 717, 1100, 1400
MaeII	ACGT	5 cut(s)	273, 1156, 1488, 1569, 1689
MaeIII	GTNAC	3 cut(s)	719, 926, 1198
MalI	GATC	7 cut(s)	78, 1062, 1128, 1442, 1469, 1595, 1796
MboI	GATC	7 cut(s)	76, 1060, 1126, 1440, 1467, 1593, 1794
MflI	RGATCY	2 cut(s)	76, 1467
MhlI	GDGCHC	5 cut(s)	210, 455, 1575, 1644, 1725
MluCI	AATT	6 cut(s)	638, 922, 937, 1108, 1262, 1475
MmeI	TCCRAC	4 cut(s)	174, 445, 1374, 1482
MroI	TCCGGA	1 cut(s)	192
MslI	CAYNNNNRTG	1 cut(s)	1553
MspA1I	CMGCKG	2 cut(s)	337, 1736
Mva1269I	GAATGC	2 cut(s)	637, 1274
MvaI	CCWGG	6 cut(s)	236, 260, 284, 770, 1318, 1891
MvnI	CGCG	3 cut(s)	143, 320, 368
MwoI	GCNNNNNNNGC	9 cut(s)	98, 101, 104, 196, 205, 317, 331, 412, 755
NciI	CCSGG	6 cut(s)	74, 137, 269, 534, 1654, 1877
NdeII	GATC	7 cut(s)	76, 1060, 1126, 1440, 1467, 1593, 1794
NlaIII	CATG	4 cut(s)	92, 392, 518, 1552
NlaIV	GGNNCC	6 cut(s)	78, 351, 512, 564, 1041, 1507
NmeAIII	GCCGAG	3 cut(s)	150, 229, 436
NmuCI	GTSAC	2 cut(s)	719, 926
NsbI	TGCGCA	1 cut(s)	807
NspV	TTCGAA	1 cut(s)	1473
PceI	AGGCCT	1 cut(s)	1517
PcsI	WCGNNNNNNNCGW	4 cut(s)	147, 177, 1381, 1403
PctI	GAATGC	2 cut(s)	637, 1274
PfeI	GAWTC	3 cut(s)	328, 682, 1346
PflMI	CCANNNNNTGG	1 cut(s)	1783
PfoI	TCCNGGA	2 cut(s)	258, 1875
PinAI	ACCGGT	1 cut(s)	293
Ppu21I	YACGTR	1 cut(s)	1690
PpuMI	RGGWCCY	1 cut(s)	562
PshAI	GACNNNNGTC	1 cut(s)	230
Psp124BI	GAGCTC	2 cut(s)	210, 1725
Psp5II	RGGWCCY	1 cut(s)	562
Psp6I	CCWGG	6 cut(s)	234, 258, 282, 768, 1316, 1889
PspFI	CCCAGC	2 cut(s)	277, 993
PspGI	CCWGG	6 cut(s)	234, 258, 282, 768, 1316, 1889
PspN4I	GGNNCC	6 cut(s)	78, 351, 512, 564, 1041, 1507
PspPI	GGNCC	9 cut(s)	115, 232, 265, 280, 286, 350, 510, 562, 1789
PspPPI	RGGWCCY	1 cut(s)	562
PstI	CTGCAG	2 cut(s)	133, 336
PstNI	CAGNNNCTG	2 cut(s)	340, 694
PsuI	RGATCY	2 cut(s)	76, 1467
PvuII	CAGCTG	1 cut(s)	1736
RsaI	GTAC	4 cut(s)	401, 761, 1159, 1688
RsaNI	GTAC	4 cut(s)	400, 760, 1158, 1687
RseI	CAYNNNNRTG	1 cut(s)	1553
Rsr2I	CGGWCCG	1 cut(s)	1789
RsrII	CGGWCCG	1 cut(s)	1789
SacI	GAGCTC	2 cut(s)	210, 1725
Sau3AI	GATC	7 cut(s)	76, 1060, 1126, 1440, 1467, 1593, 1794
Sau96I	GGNCC	9 cut(s)	115, 232, 265, 280, 286, 350, 510, 562, 1789
SduI	GDGCHC	5 cut(s)	210, 455, 1575, 1644, 1725
SfaNI	GCATC	4 cut(s)	68, 255, 1545, 1624
SfcI	CTRYAG	3 cut(s)	30, 129, 332
SfuI	TTCGAA	1 cut(s)	1473
SinI	GGWCC	4 cut(s)	115, 232, 562, 1789
SmiMI	CAYNNNNRTG	1 cut(s)	1553
SmlI	CTYRAG	2 cut(s)	119, 1289
SmoI	CTYRAG	2 cut(s)	119, 1289
SpeI	ACTAGT	1 cut(s)	716
Sse9I	AATT	6 cut(s)	638, 922, 937, 1108, 1262, 1475
SseBI	AGGCCT	1 cut(s)	1517
SspMI	CTAG	4 cut(s)	602, 717, 1100, 1400
SstI	GAGCTC	2 cut(s)	210, 1725
StuI	AGGCCT	1 cut(s)	1517
StyI	CCWWGG	1 cut(s)	588
TaaI	ACNGT	6 cut(s)	25, 793, 1093, 1148, 1254, 1510
TaiI	ACGT	5 cut(s)	276, 1159, 1491, 1572, 1692
TaqI	TCGA	2 cut(s)	866, 1473
TaqII	GACCGA	1 cut(s)	200
TasI	AATT	6 cut(s)	638, 922, 937, 1108, 1262, 1475
TauI	GCSGC	8 cut(s)	95, 98, 101, 104, 107, 303, 314, 340
TfiI	GAWTC	3 cut(s)	328, 682, 1346
TscAI	CASTG	4 cut(s)	28, 674, 738, 1050
TseFI	GTSAC	2 cut(s)	719, 926
TseI	GCWGC	7 cut(s)	187, 322, 334, 439, 1219, 1733, 1868
Tsp45I	GTSAC	2 cut(s)	719, 926
TspDTI	ATGAA	5 cut(s)	792, 811, 887, 1214, 1349
TspGWI	ACGGA	4 cut(s)	21, 1289, 1390, 1771
TspRI	CASTG	4 cut(s)	28, 674, 738, 1050
Van91I	CCANNNNNTGG	1 cut(s)	1783
VneI	GTGCAC	1 cut(s)	1571
VpaK11BI	GGWCC	4 cut(s)	115, 232, 562, 1789
XapI	RAATTY	3 cut(s)	638, 937, 1262
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1075
XspI	CTAG	4 cut(s)	602, 717, 1100, 1400

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.