Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0010462

Physical Location & Seq

Genomic Coordinates Reverse (-)

19051838 .. 19054133

Gene Structure 2296 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000032788

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1479 bp

ATGGACGGAGAAGAAGAAGCCAAGGTATCTATAGACTTCCCTCTCTTCAAACCCAGAGCATCTAAACCCGCCCCGAAACCCGACCCGGATCCGATAACCCATGTCTCGGAACCGGACCTTGAGAGGTCCGCAGACCCAGACGCCAACTCCGACGCCAAGTTCACCGACCTCGGCCTCGCCGACTGGCTGCTCCGGACGCTCGCCGAGCTCGGTCTGAAGAAGCCGACGCCGGTCCAGGCCAACTGCATCCCCAAAATCCTGGAGGGCCGGGACGTGCTGGGCCTGGCCCAGACCGGTAGCGGCAAAACGGCGGCGTTCGCGCTGCCGATTCTGCAGCGGCTGGCGGAGGGGCCTTATGGGGTTTTCGCGCTGGTGATGACGCCGACGCATGAGCTGGCGTACCAGCTCGCCGAGCAGTTCAGGGCTTTCGGGTCGTCGCTGCATTTGAGGTGCTCGGTGATTGTTGGAGGTATGGATAAGCTGAAGCAAGCTCAGAGCTTGATGAGTAGGCCCCATGTGGTGATTGCCACGCCCGGTAGGGTTAGGGATTTGCTTCAGGAGGACCCTGATTTTCCCTCTGTTTTCTCCAAGGCCAAGTTCCTAGTGCTGGATGAAGCAGATAGGTTATTGAATTCTGAATTTGAGGCCGATTTGAGAGTGGTGTTTCAGTGCTTGCCGAAGAATCGGCAGACTCTGTTGTTCTCTGCAACAATGACTAGTGACCTGCAAGCACTGATTGAAGTTTCTGCAAATAAGGCGTACTTTTACCGGGCTTATGAAGGTCTCAGGACGGTTGGCACTCTTGCGCAGAAATATGTTTTCATACCCCGTGAAGCGAAAGAAGTTTATTTGGTGTATATTTTGTCGAAAATGAAAGATATGGGTATTCGTTCAGCCATCGTGTTTGTCTCAACCCTATGGAATTGTCACTTATTGAATTTTTTACTGGAGGAGCTTGGTCAAAGTGTTTCAGCATTGCACTCATCCAAATCCCAGCCTCTTCGACTTTCTGCATTACATAAGTTCAAATCTGGACAGGTTCCAGTGTTGATTGCAACTGATCTTGCCAATCGTGGTTTGGATATTCCTACGGTTGACCTAGTAATCAATTATGATATTCCAAGTGATCCAAAGGATTATGTTCACCGTGTGGGACGTACTGCAAGAGCAGGCAGAGGAGGTTTAGCTGTCAGCGTTGTTACCCAACTCGATGTAAAGCTGCTTCATAAAATAGAAGAAGAAGTTGGGGAACAGTTGGTAGAATTTGAATGCAAGGAGAACGAGGTTCTTGAGGATATTCGTAAGGTTTACACAGCCAGGCGTGTCGCAAAGATGAAGATGATGGAATCCAACTTTGTAGAAAGAGAGAAAAAACGGAAAGAACGAACACTAAAGACGCTAGAAGCGAAAGGTTTATTGAAAAAAAGGAGTAAGAAGAAGAAGAGAGAAAAATCAACCGTTGAATTGGCTCCCGTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0007275 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009553 GO:0009561 GO:0009791 GO:0009987 GO:0010467 GO:0010501 GO:0016070 GO:0016072 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022613 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0042623 GO:0042995 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0048229 GO:0048856 GO:0070013 GO:0070035 GO:0071704 GO:0071840 GO:0090304 GO:0090406 GO:0120025 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

493

Amino Acids

54.97

Weight (kDa)

8.98

Isoelectric Point (pI)

37.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ResIII	PF04851	74 - 238	8.2e-07	Type III restriction enzyme, res subunit
DEAD	PF00270	76 - 244	4.6e-48	DEAD/DEAH box helicase
Helicase_C	PF00271	293 - 391	1.3e-26	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010462)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G16280
fragaria_vesca	FvH4_3g14750 FvH4_3g14750 FvH4_3g14750
malus_domestica	MD10G1209000.v1.1
prunus_persica	Prupe.4G132500_v2.0.a1
pyrus_communis	pycom10g17780 pycom13g14880
rosa_chinensis	RchiOBHm_Chr5g0024261
rosa_laevigata	RLG00000032788
rosa_multiflora	Rmu_sc0002344.1_g000004 Rmu_sc0018255.1_g000002
rosa_roxburghii	Rroxscaffold_1G00054920 Rroxscaffold_1G00055760
rosa_rugosa	Rorug05G0082900
rosa_samantha	Rh5AG173300 Rh5BG170200 Rh5CG186300 Rh5DG172800
rosa_wichuraiana	Rw0G000850 Rw5G015650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	807
Acc36I	ACCTGC	1 cut(s)	732
AccII	CGCG	2 cut(s)	320, 368
AccIII	TCCGGA	1 cut(s)	192
AciI	CCGC	6 cut(s)	69, 129, 300, 311, 337, 344
AclWI	GGATC	3 cut(s)	83, 96, 1121
AcsI	RAATTY	4 cut(s)	631, 638, 937, 1262
AcuI	CTGAAG	3 cut(s)	236, 503, 539
AcyI	GRCGYC	4 cut(s)	141, 153, 227, 380
AdeI	CACNNNGTG	1 cut(s)	1150
AfaI	GTAC	3 cut(s)	401, 761, 1159
AfiI	CCNNNNNNNGG	2 cut(s)	106, 607
AgeI	ACCGGT	1 cut(s)	293
AgsI	TTSAA	8 cut(s)	49, 631, 740, 937, 1027, 1268, 1420, 1463
AhlI	ACTAGT	1 cut(s)	716
AjiI	CACGTC	1 cut(s)	274
AjnI	CCWGG	4 cut(s)	234, 258, 282, 1316
AjuI	GAANNNNNNNTTGG	4 cut(s)	581, 613, 1227, 1259
AluBI	AGCT	9 cut(s)	208, 394, 406, 481, 491, 498, 955, 1187, 1219
AluI	AGCT	9 cut(s)	208, 394, 406, 481, 491, 498, 955, 1187, 1219
Alw21I	GWGCWC	2 cut(s)	210, 455
Alw26I	GTCTC	3 cut(s)	109, 788, 913
AlwI	GGATC	3 cut(s)	83, 96, 1121
AlwNI	CAGNNNCTG	2 cut(s)	340, 694
Aor13HI	TCCGGA	1 cut(s)	192
AoxI	GGCC	9 cut(s)	172, 237, 265, 280, 285, 350, 509, 591, 645
ApeKI	GCWGC	5 cut(s)	187, 322, 334, 439, 1219
ApoI	RAATTY	4 cut(s)	631, 638, 937, 1262
AsiGI	ACCGGT	1 cut(s)	293
AspLEI	GCGC	3 cut(s)	322, 370, 808
AspS9I	GGNCC	9 cut(s)	115, 126, 232, 265, 280, 286, 350, 510, 562
AsuC2I	CCSGG	4 cut(s)	86, 269, 534, 770
AsuHPI	GGTGA	5 cut(s)	154, 385, 469, 532, 1136
AvaII	GGWCC	4 cut(s)	115, 126, 232, 562
BamHI	GGATCC	1 cut(s)	88
BanII	GRGCYC	1 cut(s)	210
BarI	GAAGNNNNNNTAC	2 cut(s)	1206, 1238
Bbv12I	GWGCWC	2 cut(s)	210, 455
BbvI	GCAGC	5 cut(s)	174, 309, 346, 426, 1206
BccI	CCATC	2 cut(s)	905, 1336
BceAI	ACGGC	1 cut(s)	324
BciT130I	CCWGG	4 cut(s)	236, 260, 284, 1318
BcnI	CCSGG	4 cut(s)	86, 269, 534, 770
BcoDI	GTCTC	3 cut(s)	109, 788, 913
BcuI	ACTAGT	1 cut(s)	716
BfaI	CTAG	4 cut(s)	602, 717, 1100, 1400
BfmI	CTRYAG	2 cut(s)	30, 332
BfuAI	ACCTGC	1 cut(s)	732
BisI	GCNGC	8 cut(s)	188, 301, 312, 323, 335, 338, 440, 1220
BlsI	GCNGC	8 cut(s)	189, 302, 313, 324, 336, 339, 441, 1221
Bme1390I	CCNGG	8 cut(s)	86, 236, 260, 269, 284, 534, 770, 1318
Bme18I	GGWCC	4 cut(s)	115, 126, 232, 562
BmgBI	CACGTC	1 cut(s)	274
BmgT120I	GGNCC	9 cut(s)	115, 126, 232, 265, 280, 286, 350, 510, 562
BmiI	GGNNCC	7 cut(s)	90, 111, 351, 512, 564, 1041, 1470
BmrFI	CCNGG	8 cut(s)	86, 236, 260, 269, 284, 534, 770, 1318
BmsI	GCATC	2 cut(s)	68, 255
BoxI	GACNNNNGTC	1 cut(s)	230
BpmI	CTGGAG	2 cut(s)	281, 968
BpuEI	CTTGAG	2 cut(s)	140, 1310
BpuMI	CCSGG	4 cut(s)	86, 269, 534, 770
BsaHI	GRCGYC	4 cut(s)	141, 153, 227, 380
BsaI	GGTCTC	1 cut(s)	788
BsaJI	CCNNGG	3 cut(s)	21, 169, 588
BsaWI	WCCGGW	3 cut(s)	112, 192, 293
BsaXI	ACNNNNNCTCC	2 cut(s)	131, 161
Bsc4I	CCNNNNNNNGG	2 cut(s)	106, 607
Bse118I	RCCGGY	2 cut(s)	229, 293
Bse1I	ACTGG	3 cut(s)	188, 951, 1043
Bse3DI	GCAATG	1 cut(s)	974
BseAI	TCCGGA	1 cut(s)	192
BseBI	CCWGG	4 cut(s)	236, 260, 284, 1318
BseDI	CCNNGG	3 cut(s)	21, 169, 588
BseGI	GGATG	3 cut(s)	246, 616, 983
BseLI	CCNNNNNNNGG	2 cut(s)	106, 607
BseMI	GCAATG	1 cut(s)	974
BseMII	CTCAG	2 cut(s)	506, 799
BseNI	ACTGG	3 cut(s)	188, 951, 1043
BseRI	GAGGAG	2 cut(s)	965, 1191
BseXI	GCAGC	5 cut(s)	174, 309, 346, 426, 1206
BseYI	CCCAGC	2 cut(s)	277, 993
Bsh1236I	CGCG	2 cut(s)	320, 368
BshFI	GGCC	9 cut(s)	174, 239, 267, 282, 287, 352, 511, 593, 647
BshTI	ACCGGT	1 cut(s)	293
BsiHKAI	GWGCWC	2 cut(s)	210, 455
BsiSI	CCGG	8 cut(s)	86, 113, 193, 230, 268, 294, 534, 769
BslFI	GGGAC	2 cut(s)	284, 1167
BslI	CCNNNNNNNGG	2 cut(s)	106, 607
BsmAI	GTCTC	3 cut(s)	109, 788, 913
BsmFI	GGGAC	2 cut(s)	284, 1167
BsmI	GAATGC	1 cut(s)	1274
BsnI	GGCC	9 cut(s)	174, 239, 267, 282, 287, 352, 511, 593, 647
Bso31I	GGTCTC	1 cut(s)	788
Bsp1286I	GDGCHC	2 cut(s)	210, 455
Bsp13I	TCCGGA	1 cut(s)	192
Bsp143I	GATC	3 cut(s)	88, 1060, 1126
BspACI	CCGC	6 cut(s)	69, 129, 300, 311, 337, 344
BspANI	GGCC	9 cut(s)	174, 239, 267, 282, 287, 352, 511, 593, 647
BspCNI	CTCAG	2 cut(s)	505, 798
BspEI	TCCGGA	1 cut(s)	192
BspFNI	CGCG	2 cut(s)	320, 368
BspLI	GGNNCC	7 cut(s)	90, 111, 351, 512, 564, 1041, 1470
BspMAI	CTGCAG	1 cut(s)	336
BspMI	ACCTGC	1 cut(s)	732
BspPI	GGATC	3 cut(s)	83, 96, 1121
BspTNI	GGTCTC	1 cut(s)	788
BsrDI	GCAATG	1 cut(s)	974
BsrFI	RCCGGY	2 cut(s)	229, 293
BsrI	ACTGG	3 cut(s)	188, 951, 1043
BssAI	RCCGGY	2 cut(s)	229, 293
BssECI	CCNNGG	3 cut(s)	21, 169, 588
BssMI	GATC	3 cut(s)	88, 1060, 1126
BssNI	GRCGYC	4 cut(s)	141, 153, 227, 380
BssT1I	CCWWGG	2 cut(s)	21, 588
Bst2UI	CCWGG	4 cut(s)	236, 260, 284, 1318
Bst4CI	ACNGT	5 cut(s)	793, 1093, 1148, 1254, 1459
Bst6I	CTCTTC	3 cut(s)	50, 1005, 1436
BstACI	GRCGYC	4 cut(s)	141, 153, 227, 380
BstC8I	GCNNGC	8 cut(s)	201, 342, 396, 408, 489, 674, 729, 1171
BstDEI	CTNAG	2 cut(s)	492, 785
BstF5I	GGATG	3 cut(s)	246, 616, 983
BstFNI	CGCG	2 cut(s)	320, 368
BstHHI	GCGC	3 cut(s)	322, 370, 808
BstKTI	GATC	3 cut(s)	91, 1063, 1129
BstMAI	GTCTC	3 cut(s)	109, 788, 913
BstMBI	GATC	3 cut(s)	88, 1060, 1126
BstMWI	GCNNNNNNNGC	6 cut(s)	196, 205, 317, 331, 412, 755
BstNI	CCWGG	4 cut(s)	236, 260, 284, 1318
BstPAI	GACNNNNGTC	1 cut(s)	230
BstSCI	CCNGG	8 cut(s)	84, 234, 258, 267, 282, 532, 768, 1316
BstSFI	CTRYAG	2 cut(s)	30, 332
BstUI	CGCG	2 cut(s)	320, 368
BstV1I	GCAGC	5 cut(s)	174, 309, 346, 426, 1206
BstX2I	RGATCY	1 cut(s)	88
BstXI	CCANNNNNNTGG	1 cut(s)	259
BstYI	RGATCY	1 cut(s)	88
BsuRI	GGCC	9 cut(s)	174, 239, 267, 282, 287, 352, 511, 593, 647
BtrI	CACGTC	1 cut(s)	274
BtsCI	GGATG	3 cut(s)	246, 616, 983
BtsIMutI	CAGTG	3 cut(s)	674, 731, 1050
BveI	ACCTGC	1 cut(s)	732
Cac8I	GCNNGC	8 cut(s)	201, 342, 396, 408, 489, 674, 729, 1171
CaiI	CAGNNNCTG	2 cut(s)	340, 694
CfoI	GCGC	3 cut(s)	322, 370, 808
Cfr10I	RCCGGY	2 cut(s)	229, 293
Cfr13I	GGNCC	9 cut(s)	115, 126, 232, 265, 280, 286, 350, 510, 562
CseI	GACGC	7 cut(s)	149, 161, 205, 235, 388, 394, 1405
Csp6I	GTAC	3 cut(s)	400, 760, 1158
CspAI	ACCGGT	1 cut(s)	293
CviAII	CATG	3 cut(s)	101, 389, 515
CviQI	GTAC	3 cut(s)	400, 760, 1158
DdeI	CTNAG	2 cut(s)	492, 785
DpnI	GATC	3 cut(s)	90, 1062, 1128
DpnII	GATC	3 cut(s)	88, 1060, 1126
DraIII	CACNNNGTG	1 cut(s)	1150
Eam1104I	CTCTTC	3 cut(s)	50, 1005, 1436
EarI	CTCTTC	3 cut(s)	50, 1005, 1436
EciI	GGCGGA	1 cut(s)	359
Ecl136II	GAGCTC	1 cut(s)	208
Eco130I	CCWWGG	2 cut(s)	21, 588
Eco24I	GRGCYC	1 cut(s)	210
Eco31I	GGTCTC	1 cut(s)	788
Eco47I	GGWCC	4 cut(s)	115, 126, 232, 562
Eco53kI	GAGCTC	1 cut(s)	208
Eco57I	CTGAAG	3 cut(s)	236, 503, 539
EcoICRI	GAGCTC	1 cut(s)	208
EcoO109I	RGGNCCY	3 cut(s)	350, 510, 562
EcoRI	GAATTC	1 cut(s)	631
EcoRII	CCWGG	4 cut(s)	234, 258, 282, 1316
EcoT14I	CCWWGG	2 cut(s)	21, 588
EcoT38I	GRGCYC	1 cut(s)	210
ErhI	CCWWGG	2 cut(s)	21, 588
FaeI	CATG	3 cut(s)	104, 392, 518
FalI	AAGNNNNNCTT	2 cut(s)	746, 778
FaqI	GGGAC	2 cut(s)	284, 1167
FatI	CATG	3 cut(s)	100, 388, 514
FauI	CCCGC	1 cut(s)	76
Fnu4HI	GCNGC	8 cut(s)	188, 301, 312, 323, 335, 338, 440, 1220
FokI	GGATG	3 cut(s)	233, 623, 970
FriOI	GRGCYC	1 cut(s)	210
Fsp4HI	GCNGC	8 cut(s)	188, 301, 312, 323, 335, 338, 440, 1220
FspBI	CTAG	4 cut(s)	602, 717, 1100, 1400
FspI	TGCGCA	1 cut(s)	807
GlaI	GCGC	3 cut(s)	321, 369, 807
GluI	GCNGC	8 cut(s)	188, 301, 312, 323, 335, 338, 440, 1220
GsaI	CCCAGC	2 cut(s)	281, 997
GsuI	CTGGAG	2 cut(s)	281, 968
HaeIII	GGCC	9 cut(s)	174, 239, 267, 282, 287, 352, 511, 593, 647
HapII	CCGG	8 cut(s)	86, 113, 193, 230, 268, 294, 534, 769
HgaI	GACGC	7 cut(s)	149, 161, 205, 235, 388, 394, 1405
HhaI	GCGC	3 cut(s)	322, 370, 808
Hin1I	GRCGYC	4 cut(s)	141, 153, 227, 380
Hin1II	CATG	3 cut(s)	104, 392, 518
Hin6I	GCGC	3 cut(s)	320, 368, 806
HinP1I	GCGC	3 cut(s)	320, 368, 806
HincII	GTYRAC	1 cut(s)	1096
HindII	GTYRAC	1 cut(s)	1096
HinfI	GANTC	4 cut(s)	328, 682, 691, 1346
HpaII	CCGG	8 cut(s)	86, 113, 193, 230, 268, 294, 534, 769
HphI	GGTGA	5 cut(s)	154, 385, 469, 532, 1136
Hpy166II	GTNNAC	4 cut(s)	162, 1096, 1144, 1309
Hpy188I	TCNGA	6 cut(s)	93, 109, 151, 216, 495, 637
Hpy188III	TCNNGA	5 cut(s)	193, 557, 787, 1032, 1289
Hpy8I	GTNNAC	4 cut(s)	162, 1096, 1144, 1309
Hpy99I	CGWCG	4 cut(s)	155, 229, 388, 439
HpyAV	CCTTC	1 cut(s)	773
HpyCH4III	ACNGT	5 cut(s)	793, 1093, 1148, 1254, 1459
HpyCH4IV	ACGT	2 cut(s)	273, 1156
HpyF10VI	GCNNNNNNNGC	6 cut(s)	196, 205, 317, 331, 412, 755
HpyF3I	CTNAG	2 cut(s)	492, 785
HpySE526I	ACGT	2 cut(s)	273, 1156
Hsp92I	GRCGYC	4 cut(s)	141, 153, 227, 380
Hsp92II	CATG	3 cut(s)	104, 392, 518
HspAI	GCGC	3 cut(s)	320, 368, 806
Kpn2I	TCCGGA	1 cut(s)	192
Kzo9I	GATC	3 cut(s)	88, 1060, 1126
LmnI	GCTCC	3 cut(s)	195, 952, 1474
Lsp1109I	GCAGC	5 cut(s)	174, 309, 346, 426, 1206
LweI	GCATC	2 cut(s)	68, 255
MaeI	CTAG	4 cut(s)	602, 717, 1100, 1400
MaeII	ACGT	2 cut(s)	273, 1156
MaeIII	GTNAC	3 cut(s)	719, 926, 1198
MalI	GATC	3 cut(s)	90, 1062, 1128
MboI	GATC	3 cut(s)	88, 1060, 1126
MflI	RGATCY	1 cut(s)	88
MhlI	GDGCHC	2 cut(s)	210, 455
MluCI	AATT	7 cut(s)	631, 638, 922, 937, 1108, 1262, 1463
MlyI	GAGTC	1 cut(s)	685
MmeI	TCCRAC	3 cut(s)	174, 445, 1374
MroI	TCCGGA	1 cut(s)	192
MspA1I	CMGCKG	1 cut(s)	337
MspI	CCGG	8 cut(s)	86, 113, 193, 230, 268, 294, 534, 769
MspR9I	CCNGG	8 cut(s)	86, 236, 260, 269, 284, 534, 770, 1318
Mva1269I	GAATGC	1 cut(s)	1274
MvaI	CCWGG	4 cut(s)	236, 260, 284, 1318
MvnI	CGCG	2 cut(s)	320, 368
MwoI	GCNNNNNNNGC	6 cut(s)	196, 205, 317, 331, 412, 755
NciI	CCSGG	4 cut(s)	86, 269, 534, 770
NdeII	GATC	3 cut(s)	88, 1060, 1126
NlaIII	CATG	3 cut(s)	104, 392, 518
NlaIV	GGNNCC	7 cut(s)	90, 111, 351, 512, 564, 1041, 1470
NmeAIII	GCCGAG	3 cut(s)	150, 229, 436
NmuCI	GTSAC	2 cut(s)	719, 926
NsbI	TGCGCA	1 cut(s)	807
PcsI	WCGNNNNNNNCGW	4 cut(s)	147, 177, 1381, 1403
PctI	GAATGC	1 cut(s)	1274
PfeI	GAWTC	3 cut(s)	328, 682, 1346
PfoI	TCCNGGA	1 cut(s)	258
PinAI	ACCGGT	1 cut(s)	293
PkrI	GCNGC	8 cut(s)	189, 302, 313, 324, 336, 339, 441, 1221
PleI	GAGTC	1 cut(s)	685
PpsI	GAGTC	1 cut(s)	685
PpuMI	RGGWCCY	1 cut(s)	562
PshAI	GACNNNNGTC	1 cut(s)	230
Psp124BI	GAGCTC	1 cut(s)	210
Psp5II	RGGWCCY	1 cut(s)	562
Psp6I	CCWGG	4 cut(s)	234, 258, 282, 1316
PspFI	CCCAGC	2 cut(s)	277, 993
PspGI	CCWGG	4 cut(s)	234, 258, 282, 1316
PspN4I	GGNNCC	7 cut(s)	90, 111, 351, 512, 564, 1041, 1470
PspPI	GGNCC	9 cut(s)	115, 126, 232, 265, 280, 286, 350, 510, 562
PspPPI	RGGWCCY	1 cut(s)	562
PstI	CTGCAG	1 cut(s)	336
PstNI	CAGNNNCTG	2 cut(s)	340, 694
PsuI	RGATCY	1 cut(s)	88
RsaI	GTAC	3 cut(s)	401, 761, 1159
RsaNI	GTAC	3 cut(s)	400, 760, 1158
SacI	GAGCTC	1 cut(s)	210
SatI	GCNGC	8 cut(s)	188, 301, 312, 323, 335, 338, 440, 1220
Sau3AI	GATC	3 cut(s)	88, 1060, 1126
Sau96I	GGNCC	9 cut(s)	115, 126, 232, 265, 280, 286, 350, 510, 562
SchI	GAGTC	1 cut(s)	685
ScrFI	CCNGG	8 cut(s)	86, 236, 260, 269, 284, 534, 770, 1318
SduI	GDGCHC	2 cut(s)	210, 455
SfaNI	GCATC	2 cut(s)	68, 255
SfcI	CTRYAG	2 cut(s)	30, 332
SinI	GGWCC	4 cut(s)	115, 126, 232, 562
SmlI	CTYRAG	2 cut(s)	119, 1289
SmoI	CTYRAG	2 cut(s)	119, 1289
SpeI	ACTAGT	1 cut(s)	716
Sse9I	AATT	7 cut(s)	631, 638, 922, 937, 1108, 1262, 1463
SsiI	CCGC	6 cut(s)	69, 129, 300, 311, 337, 344
SspMI	CTAG	4 cut(s)	602, 717, 1100, 1400
SstI	GAGCTC	1 cut(s)	210
StyD4I	CCNGG	8 cut(s)	84, 234, 258, 267, 282, 532, 768, 1316
StyI	CCWWGG	2 cut(s)	21, 588
TaaI	ACNGT	5 cut(s)	793, 1093, 1148, 1254, 1459
TaiI	ACGT	2 cut(s)	276, 1159
TaqI	TCGA	3 cut(s)	866, 1003, 1209
TaqII	GACCGA	1 cut(s)	200
TasI	AATT	7 cut(s)	631, 638, 922, 937, 1108, 1262, 1463
TauI	GCSGC	3 cut(s)	303, 314, 340
TfiI	GAWTC	3 cut(s)	328, 682, 1346
TscAI	CASTG	3 cut(s)	674, 738, 1050
TseFI	GTSAC	2 cut(s)	719, 926
TseI	GCWGC	5 cut(s)	187, 322, 334, 439, 1219
Tsp45I	GTSAC	2 cut(s)	719, 926
TspDTI	ATGAA	6 cut(s)	627, 792, 811, 887, 1214, 1349
TspGWI	ACGGA	2 cut(s)	21, 1390
TspRI	CASTG	3 cut(s)	674, 738, 1050
VpaK11BI	GGWCC	4 cut(s)	115, 126, 232, 562
XapI	RAATTY	4 cut(s)	631, 638, 937, 1262
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1075
XspI	CTAG	4 cut(s)	602, 717, 1100, 1400

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.