AT1G16740

Binds directly to 23S ribosomal RNA and is necessary for the in vitro assembly process of the 50S ribosomal subunit. It is not involved in the protein synthesizing functions of that subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0006084
Physical Location & Seq
Forward (+)
5727622 .. 5729115
1494 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G16740.1

Sequence Viewer

Length: 381 bp
ATGAACAAGAAGGAGATCTTCAAGCTAGCGAAGGGTTTCAGAGGAAGAGCTAAGAATTGTATAAGAATTGCGAGGGAAAGAGTCGAGAAAGCTCTGCAGTACTCTTACAGAGATAGGCGCAACAAGAAACGTGAGATGAGAGGTCTCTGGATCGAGCGCATCAACGCCGGTTCTCGTCAGCACGGTGTGAACTACGGGAACTTCATCCATGGACTGATGAAGGAGAACATCCAGCTGAACCGCAAAGTCCTCTCTGAGTTATCTATGCATGAACCTTACAGTTTCAAAGCACTTGTGGATGTCTCCCGCAAGTCGTTCCCTGGAAACAAGAACGTGGTCCAAGCATCTCGGAAAGTAGACATTTCATCCATCAATGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L20

Protein Analysis

126

Amino Acids

14.72

Weight (kDa)

10.74

Isoelectric Point (pI)

37.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L20 PF00453 3 - 98 2.8e-41 Ribosomal protein L20
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 357
AciI CCGC 2 cut(s) 241, 307
AclWI GGATC 1 cut(s) 158
AdeI CACNNNGTG 1 cut(s) 187
AfaI GTAC 1 cut(s) 101
AgsI TTSAA 2 cut(s) 22, 286
AjnI CCWGG 1 cut(s) 319
AluBI AGCT 4 cut(s) 25, 50, 92, 235
AluI AGCT 4 cut(s) 25, 50, 92, 235
Alw26I GTCTC 2 cut(s) 149, 307
AlwI GGATC 1 cut(s) 158
Asp700I GAANNNNTTC 1 cut(s) 35
AspLEI GCGC 2 cut(s) 120, 159
AspS9I GGNCC 1 cut(s) 337
AsuNHI GCTAGC 1 cut(s) 25
AvaII GGWCC 1 cut(s) 337
BccI CCATC 1 cut(s) 377
BciT130I CCWGG 1 cut(s) 321
BcoDI GTCTC 2 cut(s) 149, 307
BfaI CTAG 2 cut(s) 26, 379
BfmI CTRYAG 1 cut(s) 95
BglII AGATCT 1 cut(s) 15
BmcAI AGTACT 1 cut(s) 101
Bme1390I CCNGG 1 cut(s) 321
Bme18I GGWCC 1 cut(s) 337
BmgT120I GGNCC 1 cut(s) 337
BmrFI CCNGG 1 cut(s) 321
BmsI GCATC 2 cut(s) 168, 353
BmtI GCTAGC 1 cut(s) 29
BsaI GGTCTC 1 cut(s) 149
BsaJI CCNNGG 2 cut(s) 208, 319
Bse118I RCCGGY 1 cut(s) 167
BseBI CCWGG 1 cut(s) 321
BseDI CCNNGG 2 cut(s) 208, 319
BseGI GGATG 4 cut(s) 204, 228, 304, 365
BseMII CTCAG 1 cut(s) 246
BsiSI CCGG 1 cut(s) 168
BsmAI GTCTC 2 cut(s) 149, 307
Bso31I GGTCTC 1 cut(s) 149
Bsp143I GATC 2 cut(s) 15, 150
Bsp19I CCATGG 1 cut(s) 208
BspACI CCGC 2 cut(s) 241, 307
BspCNI CTCAG 1 cut(s) 247
BspMAI CTGCAG 1 cut(s) 99
BspOI GCTAGC 1 cut(s) 29
BspPI GGATC 1 cut(s) 158
BspQI GCTCTTC 1 cut(s) 40
BspTNI GGTCTC 1 cut(s) 149
BsrFI RCCGGY 1 cut(s) 167
BssAI RCCGGY 1 cut(s) 167
BssECI CCNNGG 2 cut(s) 208, 319
BssMI GATC 2 cut(s) 15, 150
BssT1I CCWWGG 1 cut(s) 208
Bst2UI CCWGG 1 cut(s) 321
Bst4CI ACNGT 2 cut(s) 185, 281
Bst6I CTCTTC 1 cut(s) 40
BstC8I GCNNGC 1 cut(s) 27
BstDEI CTNAG 2 cut(s) 51, 255
BstDSI CCRYGG 1 cut(s) 208
BstF5I GGATG 4 cut(s) 204, 228, 304, 365
BstHHI GCGC 2 cut(s) 120, 159
BstKTI GATC 2 cut(s) 18, 153
BstMAI GTCTC 2 cut(s) 149, 307
BstMBI GATC 2 cut(s) 15, 150
BstNI CCWGG 1 cut(s) 321
BstSCI CCNGG 1 cut(s) 319
BstSFI CTRYAG 1 cut(s) 95
BstX2I RGATCY 1 cut(s) 15
BstYI RGATCY 1 cut(s) 15
BtgI CCRYGG 1 cut(s) 208
BtsCI GGATG 4 cut(s) 204, 228, 304, 365
Cac8I GCNNGC 1 cut(s) 27
CfoI GCGC 2 cut(s) 120, 159
Cfr10I RCCGGY 1 cut(s) 167
Cfr13I GGNCC 1 cut(s) 337
Csp6I GTAC 1 cut(s) 100
CviAII CATG 2 cut(s) 209, 269
CviJI RGCY 4 cut(s) 25, 50, 92, 235
CviKI_1 RGCY 4 cut(s) 25, 50, 92, 235
CviQI GTAC 1 cut(s) 100
DdeI CTNAG 2 cut(s) 51, 255
DpnI GATC 2 cut(s) 17, 152
DpnII GATC 2 cut(s) 15, 150
DraIII CACNNNGTG 1 cut(s) 187
Eam1104I CTCTTC 1 cut(s) 40
EarI CTCTTC 1 cut(s) 40
Eco130I CCWWGG 1 cut(s) 208
Eco31I GGTCTC 1 cut(s) 149
Eco47I GGWCC 1 cut(s) 337
EcoRII CCWGG 1 cut(s) 319
EcoT14I CCWWGG 1 cut(s) 208
EcoT22I ATGCAT 1 cut(s) 270
ErhI CCWWGG 1 cut(s) 208
FaeI CATG 2 cut(s) 212, 272
FaiI YATR 4 cut(s) 62, 210, 266, 270
FalI AAGNNNNNCTT 1 cut(s) 34
FatI CATG 2 cut(s) 208, 268
FauI CCCGC 1 cut(s) 314
FblI GTMKAC 1 cut(s) 357
FokI GGATG 4 cut(s) 191, 215, 311, 352
FspBI CTAG 2 cut(s) 26, 379
GlaI GCGC 2 cut(s) 119, 158
HapII CCGG 1 cut(s) 168
HhaI GCGC 2 cut(s) 120, 159
Hin1II CATG 2 cut(s) 212, 272
Hin6I GCGC 2 cut(s) 118, 157
HinP1I GCGC 2 cut(s) 118, 157
HinfI GANTC 1 cut(s) 81
HpaII CCGG 1 cut(s) 168
Hpy166II GTNNAC 2 cut(s) 190, 358
Hpy188I TCNGA 3 cut(s) 41, 256, 351
Hpy188III TCNNGA 2 cut(s) 85, 148
Hpy8I GTNNAC 2 cut(s) 190, 358
HpyAV CCTTC 3 cut(s) 4, 25, 214
HpyCH4III ACNGT 2 cut(s) 185, 281
HpyCH4IV ACGT 2 cut(s) 130, 333
HpyCH4V TGCA 2 cut(s) 97, 268
HpyF3I CTNAG 2 cut(s) 51, 255
HpySE526I ACGT 2 cut(s) 130, 333
Hsp92II CATG 2 cut(s) 212, 272
HspAI GCGC 2 cut(s) 118, 157
Kzo9I GATC 2 cut(s) 15, 150
LguI GCTCTTC 1 cut(s) 40
LpnPI CCDG 5 cut(s) 133, 181, 245, 306, 333
LweI GCATC 2 cut(s) 168, 353
MaeI CTAG 2 cut(s) 26, 379
MaeII ACGT 2 cut(s) 130, 333
MalI GATC 2 cut(s) 17, 152
MboI GATC 2 cut(s) 15, 150
MboII GAAGA 2 cut(s) 10, 57
MflI RGATCY 1 cut(s) 15
MluCI AATT 2 cut(s) 55, 66
MlyI GAGTC 1 cut(s) 90
MnlI CCTC 4 cut(s) 35, 66, 134, 260
Mph1103I ATGCAT 1 cut(s) 270
MroXI GAANNNNTTC 1 cut(s) 35
MspA1I CMGCKG 1 cut(s) 235
MspI CCGG 1 cut(s) 168
MspR9I CCNGG 1 cut(s) 321
MvaI CCWGG 1 cut(s) 321
NcoI CCATGG 1 cut(s) 208
NdeII GATC 2 cut(s) 15, 150
NheI GCTAGC 1 cut(s) 25
NlaIII CATG 2 cut(s) 212, 272
NsiI ATGCAT 1 cut(s) 270
PciSI GCTCTTC 1 cut(s) 40
PdmI GAANNNNTTC 1 cut(s) 35
PleI GAGTC 1 cut(s) 89
PpsI GAGTC 1 cut(s) 89
Psp6I CCWGG 1 cut(s) 319
PspGI CCWGG 1 cut(s) 319
PspPI GGNCC 1 cut(s) 337
PstI CTGCAG 1 cut(s) 99
PsuI RGATCY 1 cut(s) 15
PvuII CAGCTG 1 cut(s) 235
RsaI GTAC 1 cut(s) 101
RsaNI GTAC 1 cut(s) 100
SapI GCTCTTC 1 cut(s) 40
Sau3AI GATC 2 cut(s) 15, 150
Sau96I GGNCC 1 cut(s) 337
ScaI AGTACT 1 cut(s) 101
SchI GAGTC 1 cut(s) 90
ScrFI CCNGG 1 cut(s) 321
SetI ASST 8 cut(s) 27, 52, 94, 133, 145, 237, 277, 336
SfaNI GCATC 2 cut(s) 168, 353
SfcI CTRYAG 1 cut(s) 95
SinI GGWCC 1 cut(s) 337
Sse9I AATT 2 cut(s) 55, 66
SsiI CCGC 2 cut(s) 241, 307
SspMI CTAG 2 cut(s) 26, 379
StyD4I CCNGG 1 cut(s) 319
StyI CCWWGG 1 cut(s) 208
TaaI ACNGT 2 cut(s) 185, 281
TaiI ACGT 2 cut(s) 133, 336
TaqI TCGA 2 cut(s) 84, 153
TasI AATT 2 cut(s) 55, 66
TatI WGTACW 1 cut(s) 99
TspDTI ATGAA 5 cut(s) 17, 193, 233, 285, 354
VpaK11BI GGWCC 1 cut(s) 337
XmiI GTMKAC 1 cut(s) 357
XmnI GAANNNNTTC 1 cut(s) 35
XspI CTAG 2 cut(s) 26, 379
ZrmI AGTACT 1 cut(s) 101
Zsp2I ATGCAT 1 cut(s) 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.