MD10G1216300.v1.1

Binds directly to 23S ribosomal RNA and is necessary for the in vitro assembly process of the 50S ribosomal subunit. It is not involved in the protein synthesizing functions of that subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0006084
Physical Location & Seq
Reverse (-)
31484756 .. 31487956
3201 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1216300.v1.1.491

Sequence Viewer

Length: 378 bp
ATGAATAAGGGTAAGATTTTCAAGCTAGCCAAGGGCTTCAGGGGAAGGGCCAAGAATTGCATAAGGATTGCCAGAGAGAGGGTAGAGAAGGCCTTGCAGTATTCTTACAGGGATCGCCGCACCAAGAAGCGAGACATGCGCTCCCTGTGGATCCAGCGTATCAATGCTGGCACCCGCATCCATGGAGTTAACTATGGAAACTTCATGCATGGGTTGATGAAGGAGAACATTCAGCTGAACAGGAAAGTTCTGTCAGAGCTGTCGATGCACGAACCACACAGTTTCAAGTCCCTGGTAGACATTTCTCGCAGCGCCTTCCCTGGAAACAAGAACGCGGTGCCTGCTCCTCAGAAAAAAGGCCTTCAAATTCTTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L20

Protein Analysis

126

Amino Acids

14.49

Weight (kDa)

11.22

Isoelectric Point (pI)

44.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L20 PF00453 3 - 98 8.1e-42 Ribosomal protein L20
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 170, 337
AccI GTMKAC 1 cut(s) 297
AccII CGCG 1 cut(s) 335
AciI CCGC 3 cut(s) 118, 175, 335
AclWI GGATC 3 cut(s) 120, 145, 158
AcsI RAATTY 1 cut(s) 366
AcuI CTGAAG 1 cut(s) 22
AfiI CCNNNNNNNGG 1 cut(s) 78
AgsI TTSAA 3 cut(s) 22, 286, 365
AjnI CCWGG 2 cut(s) 291, 319
AluBI AGCT 3 cut(s) 25, 235, 259
AluI AGCT 3 cut(s) 25, 235, 259
Alw26I GTCTC 1 cut(s) 126
AlwI GGATC 3 cut(s) 120, 145, 158
AoxI GGCC 3 cut(s) 48, 90, 358
ApeKI GCWGC 1 cut(s) 309
ApoI RAATTY 1 cut(s) 366
AspLEI GCGC 2 cut(s) 141, 314
AspS9I GGNCC 1 cut(s) 48
AsuNHI GCTAGC 1 cut(s) 25
BamHI GGATCC 1 cut(s) 150
BanI GGYRCC 2 cut(s) 170, 337
BbvI GCAGC 1 cut(s) 321
BciT130I CCWGG 2 cut(s) 293, 321
BcoDI GTCTC 1 cut(s) 126
BfaI CTAG 1 cut(s) 26
BfoI RGCGCY 1 cut(s) 315
BisI GCNGC 2 cut(s) 118, 310
BlsI GCNGC 2 cut(s) 119, 311
Bme1390I CCNGG 2 cut(s) 293, 321
BmgT120I GGNCC 1 cut(s) 48
BmiI GGNNCC 3 cut(s) 152, 172, 339
BmrFI CCNGG 2 cut(s) 293, 321
BmsI GCATC 2 cut(s) 186, 255
BmtI GCTAGC 1 cut(s) 29
BsaJI CCNNGG 4 cut(s) 30, 181, 291, 319
BsaXI ACNNNNNCTCC 2 cut(s) 125, 155
Bsc4I CCNNNNNNNGG 1 cut(s) 78
BseBI CCWGG 2 cut(s) 293, 321
BseDI CCNNGG 4 cut(s) 30, 181, 291, 319
BseGI GGATG 1 cut(s) 177
BseLI CCNNNNNNNGG 1 cut(s) 78
BseMII CTCAG 1 cut(s) 362
BseRI GAGGAG 1 cut(s) 336
BseXI GCAGC 1 cut(s) 321
Bsh1236I CGCG 1 cut(s) 335
BshFI GGCC 3 cut(s) 50, 92, 360
BshNI GGYRCC 2 cut(s) 170, 337
BslFI GGGAC 1 cut(s) 274
BslI CCNNNNNNNGG 1 cut(s) 78
BsmAI GTCTC 1 cut(s) 126
BsmFI GGGAC 1 cut(s) 274
BsnI GGCC 3 cut(s) 50, 92, 360
Bsp143I GATC 2 cut(s) 112, 150
Bsp19I CCATGG 1 cut(s) 181
BspACI CCGC 3 cut(s) 118, 175, 335
BspANI GGCC 3 cut(s) 50, 92, 360
BspCNI CTCAG 1 cut(s) 361
BspFNI CGCG 1 cut(s) 335
BspLI GGNNCC 3 cut(s) 152, 172, 339
BspOI GCTAGC 1 cut(s) 29
BspPI GGATC 3 cut(s) 120, 145, 158
BspT107I GGYRCC 2 cut(s) 170, 337
BssECI CCNNGG 4 cut(s) 30, 181, 291, 319
BssMI GATC 2 cut(s) 112, 150
BssT1I CCWWGG 2 cut(s) 30, 181
Bst2UI CCWGG 2 cut(s) 293, 321
Bst4CI ACNGT 1 cut(s) 281
BstC8I GCNNGC 3 cut(s) 27, 169, 342
BstDEI CTNAG 1 cut(s) 348
BstDSI CCRYGG 1 cut(s) 181
BstF5I GGATG 1 cut(s) 177
BstFNI CGCG 1 cut(s) 335
BstH2I RGCGCY 1 cut(s) 315
BstHHI GCGC 2 cut(s) 141, 314
BstKTI GATC 2 cut(s) 115, 153
BstMAI GTCTC 1 cut(s) 126
BstMBI GATC 2 cut(s) 112, 150
BstMWI GCNNNNNNNGC 3 cut(s) 136, 265, 341
BstNI CCWGG 2 cut(s) 293, 321
BstNSI RCATGY 1 cut(s) 139
BstSCI CCNGG 2 cut(s) 291, 319
BstUI CGCG 1 cut(s) 335
BstV1I GCAGC 1 cut(s) 321
BstX2I RGATCY 1 cut(s) 150
BstYI RGATCY 1 cut(s) 150
BsuRI GGCC 3 cut(s) 50, 92, 360
BtgI CCRYGG 1 cut(s) 181
BtsCI GGATG 1 cut(s) 177
Cac8I GCNNGC 3 cut(s) 27, 169, 342
CfoI GCGC 2 cut(s) 141, 314
Cfr13I GGNCC 1 cut(s) 48
CviAII CATG 4 cut(s) 136, 182, 205, 209
CviJI RGCY 8 cut(s) 25, 29, 36, 50, 92, 235, 259, 360
CviKI_1 RGCY 8 cut(s) 25, 29, 36, 50, 92, 235, 259, 360
DdeI CTNAG 1 cut(s) 348
DpnI GATC 2 cut(s) 114, 152
DpnII GATC 2 cut(s) 112, 150
Eco130I CCWWGG 2 cut(s) 30, 181
Eco147I AGGCCT 2 cut(s) 92, 360
Eco57I CTGAAG 1 cut(s) 22
EcoRII CCWGG 2 cut(s) 291, 319
EcoT14I CCWWGG 2 cut(s) 30, 181
EcoT22I ATGCAT 1 cut(s) 210
ErhI CCWWGG 2 cut(s) 30, 181
FaeI CATG 4 cut(s) 139, 185, 208, 212
FaiI YATR 6 cut(s) 62, 137, 183, 195, 206, 210
FaqI GGGAC 1 cut(s) 274
FatI CATG 4 cut(s) 135, 181, 204, 208
FauI CCCGC 1 cut(s) 182
FblI GTMKAC 1 cut(s) 297
Fnu4HI GCNGC 2 cut(s) 118, 310
FokI GGATG 1 cut(s) 164
Fsp4HI GCNGC 2 cut(s) 118, 310
FspBI CTAG 1 cut(s) 26
GlaI GCGC 2 cut(s) 140, 313
GluI GCNGC 2 cut(s) 118, 310
HaeII RGCGCY 1 cut(s) 315
HaeIII GGCC 3 cut(s) 50, 92, 360
HhaI GCGC 2 cut(s) 141, 314
Hin1II CATG 4 cut(s) 139, 185, 208, 212
Hin6I GCGC 2 cut(s) 139, 312
HinP1I GCGC 2 cut(s) 139, 312
HincII GTYRAC 1 cut(s) 190
HindII GTYRAC 1 cut(s) 190
HpaI GTTAAC 1 cut(s) 190
Hpy166II GTNNAC 2 cut(s) 190, 298
Hpy188I TCNGA 2 cut(s) 256, 351
Hpy8I GTNNAC 2 cut(s) 190, 298
HpyAV CCTTC 5 cut(s) 39, 82, 214, 325, 371
HpyCH4III ACNGT 1 cut(s) 281
HpyCH4V TGCA 4 cut(s) 60, 97, 208, 268
HpyF10VI GCNNNNNNNGC 3 cut(s) 136, 265, 341
HpyF3I CTNAG 1 cut(s) 348
Hsp92II CATG 4 cut(s) 139, 185, 208, 212
HspAI GCGC 2 cut(s) 139, 312
KspAI GTTAAC 1 cut(s) 190
Kzo9I GATC 2 cut(s) 112, 150
LmnI GCTCC 2 cut(s) 146, 349
Lsp1109I GCAGC 1 cut(s) 321
LweI GCATC 2 cut(s) 186, 255
MaeI CTAG 1 cut(s) 26
MalI GATC 2 cut(s) 114, 152
MboI GATC 2 cut(s) 112, 150
MflI RGATCY 1 cut(s) 150
MluCI AATT 2 cut(s) 55, 366
MnlI CCTC 2 cut(s) 72, 357
Mph1103I ATGCAT 1 cut(s) 210
MseI TTAA 1 cut(s) 189
MspA1I CMGCKG 1 cut(s) 235
MspR9I CCNGG 2 cut(s) 293, 321
MvaI CCWGG 2 cut(s) 293, 321
MvnI CGCG 1 cut(s) 335
MwoI GCNNNNNNNGC 3 cut(s) 136, 265, 341
NcoI CCATGG 1 cut(s) 181
NdeII GATC 2 cut(s) 112, 150
NheI GCTAGC 1 cut(s) 25
NlaIII CATG 4 cut(s) 139, 185, 208, 212
NlaIV GGNNCC 3 cut(s) 152, 172, 339
NsiI ATGCAT 1 cut(s) 210
NspI RCATGY 1 cut(s) 139
PceI AGGCCT 2 cut(s) 92, 360
PkrI GCNGC 2 cut(s) 119, 311
Psp6I CCWGG 2 cut(s) 291, 319
PspGI CCWGG 2 cut(s) 291, 319
PspN4I GGNNCC 3 cut(s) 152, 172, 339
PspPI GGNCC 1 cut(s) 48
PsuI RGATCY 1 cut(s) 150
PvuII CAGCTG 1 cut(s) 235
SaqAI TTAA 1 cut(s) 189
SatI GCNGC 2 cut(s) 118, 310
Sau3AI GATC 2 cut(s) 112, 150
Sau96I GGNCC 1 cut(s) 48
ScrFI CCNGG 2 cut(s) 293, 321
SetI ASST 3 cut(s) 27, 237, 261
SfaNI GCATC 2 cut(s) 186, 255
Sse9I AATT 2 cut(s) 55, 366
SseBI AGGCCT 2 cut(s) 92, 360
SsiI CCGC 3 cut(s) 118, 175, 335
SspMI CTAG 1 cut(s) 26
StuI AGGCCT 2 cut(s) 92, 360
StyD4I CCNGG 2 cut(s) 291, 319
StyI CCWWGG 2 cut(s) 30, 181
TaaI ACNGT 1 cut(s) 281
TaqI TCGA 1 cut(s) 263
TasI AATT 2 cut(s) 55, 366
TauI GCSGC 1 cut(s) 120
Tru1I TTAA 1 cut(s) 189
Tru9I TTAA 1 cut(s) 189
TseI GCWGC 1 cut(s) 309
TspDTI ATGAA 3 cut(s) 17, 193, 233
XapI RAATTY 1 cut(s) 366
XceI RCATGY 1 cut(s) 139
XmiI GTMKAC 1 cut(s) 297
XspI CTAG 1 cut(s) 26
Zsp2I ATGCAT 1 cut(s) 210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.