MD07G1169200.v1.1

Binds directly to 23S ribosomal RNA and is necessary for the in vitro assembly process of the 50S ribosomal subunit. It is not involved in the protein synthesizing functions of that subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0006084
Physical Location & Seq
Forward (+)
24374708 .. 24377598
2891 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1169200.v1.1.491

Sequence Viewer

Length: 318 bp
ATGCGCTCCCTGTGGATCCAGCGTATCAATGCTGGCACCCGCATCCATGGAATTATGTTGAGGTCGTGTATTGTTGTCAAACCCGATTTATTGAAAATTGTGGATGTGGGAGCTGTTCAATATACTGTTAACTATGGAAACTTCATGCATGGGTTGATGAAGGAGAACATTCAGCTGAACAGGAAAGTTCTGTCGGAGCTTTCGATGCACGAACCACATAGTTTCAAGTCCCTGGTAGACATTTCTCGCAGCGCCTTCCCTGGAAACAAGAACGTGATGCCCGCTCCTCAGAAAAAAGGCCTCCAAATTCTTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L20

Protein Analysis

106

Amino Acids

11.87

Weight (kDa)

10.17

Isoelectric Point (pI)

47.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L20 PF00453 35 - 78 1.8e-11 Ribosomal protein L20
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 35
AccBSI CCGCTC 1 cut(s) 284
AccI GTMKAC 1 cut(s) 237
AciI CCGC 2 cut(s) 40, 282
AclWI GGATC 2 cut(s) 10, 23
AcsI RAATTY 1 cut(s) 306
AgsI TTSAA 3 cut(s) 94, 119, 226
AjnI CCWGG 2 cut(s) 231, 259
AluBI AGCT 3 cut(s) 113, 175, 199
AluI AGCT 3 cut(s) 113, 175, 199
AlwI GGATC 2 cut(s) 10, 23
AoxI GGCC 1 cut(s) 298
ApeKI GCWGC 1 cut(s) 249
ApoI RAATTY 1 cut(s) 306
AspLEI GCGC 2 cut(s) 6, 254
BamHI GGATCC 1 cut(s) 15
BanI GGYRCC 1 cut(s) 35
BbvI GCAGC 1 cut(s) 261
BciT130I CCWGG 2 cut(s) 233, 261
BfoI RGCGCY 1 cut(s) 255
BisI GCNGC 1 cut(s) 250
BlsI GCNGC 1 cut(s) 251
Bme1390I CCNGG 2 cut(s) 233, 261
BmiI GGNNCC 2 cut(s) 17, 37
BmrFI CCNGG 2 cut(s) 233, 261
BmsI GCATC 3 cut(s) 51, 195, 267
BsaJI CCNNGG 3 cut(s) 46, 231, 259
BseBI CCWGG 2 cut(s) 233, 261
BseDI CCNNGG 3 cut(s) 46, 231, 259
BseGI GGATG 2 cut(s) 42, 109
BseMII CTCAG 1 cut(s) 302
BseRI GAGGAG 1 cut(s) 276
BseXI GCAGC 1 cut(s) 261
BshFI GGCC 1 cut(s) 300
BshNI GGYRCC 1 cut(s) 35
BslFI GGGAC 1 cut(s) 214
BsmFI GGGAC 1 cut(s) 214
BsnI GGCC 1 cut(s) 300
Bsp143I GATC 1 cut(s) 15
Bsp19I CCATGG 1 cut(s) 46
BspACI CCGC 2 cut(s) 40, 282
BspANI GGCC 1 cut(s) 300
BspCNI CTCAG 1 cut(s) 301
BspLI GGNNCC 2 cut(s) 17, 37
BspPI GGATC 2 cut(s) 10, 23
BspT107I GGYRCC 1 cut(s) 35
BsrBI CCGCTC 1 cut(s) 284
BssECI CCNNGG 3 cut(s) 46, 231, 259
BssMI GATC 1 cut(s) 15
BssT1I CCWWGG 1 cut(s) 46
Bst2UI CCWGG 2 cut(s) 233, 261
Bst4CI ACNGT 1 cut(s) 127
BstC8I GCNNGC 2 cut(s) 34, 282
BstDEI CTNAG 1 cut(s) 288
BstDSI CCRYGG 1 cut(s) 46
BstF5I GGATG 2 cut(s) 42, 109
BstH2I RGCGCY 1 cut(s) 255
BstHHI GCGC 2 cut(s) 6, 254
BstKTI GATC 1 cut(s) 18
BstMBI GATC 1 cut(s) 15
BstMWI GCNNNNNNNGC 1 cut(s) 205
BstNI CCWGG 2 cut(s) 233, 261
BstSCI CCNGG 2 cut(s) 231, 259
BstV1I GCAGC 1 cut(s) 261
BstX2I RGATCY 1 cut(s) 15
BstYI RGATCY 1 cut(s) 15
BsuRI GGCC 1 cut(s) 300
BtgI CCRYGG 1 cut(s) 46
BtsCI GGATG 2 cut(s) 42, 109
Cac8I GCNNGC 2 cut(s) 34, 282
CfoI GCGC 2 cut(s) 6, 254
CviAII CATG 3 cut(s) 47, 145, 149
CviJI RGCY 4 cut(s) 113, 175, 199, 300
CviKI_1 RGCY 4 cut(s) 113, 175, 199, 300
DdeI CTNAG 1 cut(s) 288
DpnI GATC 1 cut(s) 17
DpnII GATC 1 cut(s) 15
Eco130I CCWWGG 1 cut(s) 46
Eco147I AGGCCT 1 cut(s) 300
EcoRII CCWGG 2 cut(s) 231, 259
EcoT14I CCWWGG 1 cut(s) 46
EcoT22I ATGCAT 1 cut(s) 150
ErhI CCWWGG 1 cut(s) 46
FaeI CATG 3 cut(s) 50, 148, 152
FaiI YATR 7 cut(s) 48, 56, 123, 135, 146, 150, 219
FaqI GGGAC 1 cut(s) 214
FatI CATG 3 cut(s) 46, 144, 148
FauI CCCGC 2 cut(s) 47, 289
FblI GTMKAC 1 cut(s) 237
Fnu4HI GCNGC 1 cut(s) 250
FokI GGATG 2 cut(s) 29, 116
Fsp4HI GCNGC 1 cut(s) 250
GlaI GCGC 2 cut(s) 5, 253
GluI GCNGC 1 cut(s) 250
HaeII RGCGCY 1 cut(s) 255
HaeIII GGCC 1 cut(s) 300
HhaI GCGC 2 cut(s) 6, 254
Hin1II CATG 3 cut(s) 50, 148, 152
Hin6I GCGC 2 cut(s) 4, 252
HinP1I GCGC 2 cut(s) 4, 252
HincII GTYRAC 1 cut(s) 130
HindII GTYRAC 1 cut(s) 130
HpaI GTTAAC 1 cut(s) 130
Hpy166II GTNNAC 2 cut(s) 130, 238
Hpy188I TCNGA 2 cut(s) 196, 291
Hpy8I GTNNAC 2 cut(s) 130, 238
HpyAV CCTTC 2 cut(s) 154, 265
HpyCH4III ACNGT 1 cut(s) 127
HpyCH4IV ACGT 1 cut(s) 273
HpyCH4V TGCA 2 cut(s) 148, 208
HpyF10VI GCNNNNNNNGC 1 cut(s) 205
HpyF3I CTNAG 1 cut(s) 288
HpySE526I ACGT 1 cut(s) 273
Hsp92II CATG 3 cut(s) 50, 148, 152
HspAI GCGC 2 cut(s) 4, 252
KspAI GTTAAC 1 cut(s) 130
Kzo9I GATC 1 cut(s) 15
LmnI GCTCC 4 cut(s) 11, 110, 196, 289
LpnPI CCDG 8 cut(s) 18, 23, 32, 166, 218, 245, 246, 273
Lsp1109I GCAGC 1 cut(s) 261
LweI GCATC 3 cut(s) 51, 195, 267
MaeII ACGT 1 cut(s) 273
MalI GATC 1 cut(s) 17
MbiI CCGCTC 1 cut(s) 284
MboI GATC 1 cut(s) 15
MflI RGATCY 1 cut(s) 15
MluCI AATT 3 cut(s) 51, 96, 306
MmeI TCCRAC 1 cut(s) 174
MnlI CCTC 3 cut(s) 54, 297, 311
Mph1103I ATGCAT 1 cut(s) 150
MseI TTAA 1 cut(s) 129
MspA1I CMGCKG 1 cut(s) 175
MspR9I CCNGG 2 cut(s) 233, 261
MvaI CCWGG 2 cut(s) 233, 261
MwoI GCNNNNNNNGC 1 cut(s) 205
NcoI CCATGG 1 cut(s) 46
NdeII GATC 1 cut(s) 15
NlaIII CATG 3 cut(s) 50, 148, 152
NlaIV GGNNCC 2 cut(s) 17, 37
NsiI ATGCAT 1 cut(s) 150
PceI AGGCCT 1 cut(s) 300
PcsI WCGNNNNNNNCGW 1 cut(s) 200
PkrI GCNGC 1 cut(s) 251
Psp6I CCWGG 2 cut(s) 231, 259
PspGI CCWGG 2 cut(s) 231, 259
PspN4I GGNNCC 2 cut(s) 17, 37
PsuI RGATCY 1 cut(s) 15
PvuII CAGCTG 1 cut(s) 175
SaqAI TTAA 1 cut(s) 129
SatI GCNGC 1 cut(s) 250
Sau3AI GATC 1 cut(s) 15
ScrFI CCNGG 2 cut(s) 233, 261
SetI ASST 5 cut(s) 65, 115, 177, 201, 276
SfaNI GCATC 3 cut(s) 51, 195, 267
Sse9I AATT 3 cut(s) 51, 96, 306
SseBI AGGCCT 1 cut(s) 300
SsiI CCGC 2 cut(s) 40, 282
StuI AGGCCT 1 cut(s) 300
StyD4I CCNGG 2 cut(s) 231, 259
StyI CCWWGG 1 cut(s) 46
TaaI ACNGT 1 cut(s) 127
TaiI ACGT 1 cut(s) 276
TaqI TCGA 1 cut(s) 203
TasI AATT 3 cut(s) 51, 96, 306
Tru1I TTAA 1 cut(s) 129
Tru9I TTAA 1 cut(s) 129
TseI GCWGC 1 cut(s) 249
TspDTI ATGAA 2 cut(s) 133, 173
XapI RAATTY 1 cut(s) 306
XmiI GTMKAC 1 cut(s) 237
Zsp2I ATGCAT 1 cut(s) 150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.