FvH4_3g14110

Binds directly to 23S ribosomal RNA and is necessary for the in vitro assembly process of the 50S ribosomal subunit. It is not involved in the protein synthesizing functions of that subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0006084
Physical Location & Seq
Forward (+)
8471868 .. 8474282
2415 bp
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UTR
Exon/CDS
Intron
FvH4_3g14110.t1

Sequence Viewer

Length: 375 bp
ATGCATAAGGATAAGATTTTCAAGCTCGCCAAGGGATTCAGGGGGAGGGCCAAGAACTGCATCAGGATAGCCAGAGAGAGGGTGGAGAAGGCCTTGCAGTATTCCTACAGAGATCGCCGCACCAAGAAGAGAGACATGCGTGCCCTGTGGATCCAACGGATCAATGCTGGCACCCGCATTCATGGTGTTAATTATGGGAATTTCATGCATGGATTGATGAAGGAGAACATTCAGCTAAACAGGAAAGTTTTATCAGAGATTTCTATGCACGAACCATACACATTCAAGGCCCTCGTAGATGTCTCTCACAGCGCATTCCCTGGGAACAAGGCAGTGGTTCCTCCCAAAAAGGAAGGCCTCCAAATTCTTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L20

Protein Analysis

125

Amino Acids

14.48

Weight (kDa)

10.62

Isoelectric Point (pI)

40.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L20 PF00453 3 - 98 4.1e-41 Ribosomal protein L20
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 170
AciI CCGC 2 cut(s) 118, 175
AclWI GGATC 3 cut(s) 145, 158, 167
AcsI RAATTY 2 cut(s) 199, 363
AfiI CCNNNNNNNGG 1 cut(s) 78
AgsI TTSAA 2 cut(s) 22, 286
AjnI CCWGG 1 cut(s) 319
AluBI AGCT 2 cut(s) 25, 235
AluI AGCT 2 cut(s) 25, 235
Alw26I GTCTC 2 cut(s) 126, 307
AlwI GGATC 3 cut(s) 145, 158, 167
AoxI GGCC 4 cut(s) 48, 90, 288, 355
ApoI RAATTY 2 cut(s) 199, 363
AspLEI GCGC 1 cut(s) 314
AspS9I GGNCC 2 cut(s) 48, 289
BaeGI GKGCMC 1 cut(s) 145
BamHI GGATCC 1 cut(s) 150
BanI GGYRCC 1 cut(s) 170
BciT130I CCWGG 1 cut(s) 321
BcoDI GTCTC 2 cut(s) 126, 307
BfmI CTRYAG 1 cut(s) 106
BisI GCNGC 1 cut(s) 118
BlsI GCNGC 1 cut(s) 119
Bme1390I CCNGG 1 cut(s) 321
BmgT120I GGNCC 2 cut(s) 48, 289
BmiI GGNNCC 3 cut(s) 152, 172, 339
BmrFI CCNGG 1 cut(s) 321
BmsI GCATC 1 cut(s) 69
BsaJI CCNNGG 3 cut(s) 30, 319, 320
Bsc4I CCNNNNNNNGG 1 cut(s) 78
BseBI CCWGG 1 cut(s) 321
BseDI CCNNGG 3 cut(s) 30, 319, 320
BseLI CCNNNNNNNGG 1 cut(s) 78
BseSI GKGCMC 1 cut(s) 145
BshFI GGCC 4 cut(s) 50, 92, 290, 357
BshNI GGYRCC 1 cut(s) 170
BslI CCNNNNNNNGG 1 cut(s) 78
BsmAI GTCTC 2 cut(s) 126, 307
BsmI GAATGC 2 cut(s) 177, 314
BsnI GGCC 4 cut(s) 50, 92, 290, 357
Bsp1286I GDGCHC 1 cut(s) 145
Bsp143I GATC 3 cut(s) 112, 150, 159
BspACI CCGC 2 cut(s) 118, 175
BspANI GGCC 4 cut(s) 50, 92, 290, 357
BspLI GGNNCC 3 cut(s) 152, 172, 339
BspPI GGATC 3 cut(s) 145, 158, 167
BspT107I GGYRCC 1 cut(s) 170
BssECI CCNNGG 3 cut(s) 30, 319, 320
BssMI GATC 3 cut(s) 112, 150, 159
BssT1I CCWWGG 1 cut(s) 30
Bst2UI CCWGG 1 cut(s) 321
Bst6I CTCTTC 1 cut(s) 122
BstC8I GCNNGC 3 cut(s) 27, 141, 169
BstHHI GCGC 1 cut(s) 314
BstKTI GATC 3 cut(s) 115, 153, 162
BstMAI GTCTC 2 cut(s) 126, 307
BstMBI GATC 3 cut(s) 112, 150, 159
BstNI CCWGG 1 cut(s) 321
BstNSI RCATGY 1 cut(s) 139
BstSCI CCNGG 1 cut(s) 319
BstSFI CTRYAG 1 cut(s) 106
BstSLI GKGCMC 1 cut(s) 145
BstX2I RGATCY 1 cut(s) 150
BstYI RGATCY 1 cut(s) 150
BsuRI GGCC 4 cut(s) 50, 92, 290, 357
BtsI GCAGTG 1 cut(s) 339
BtsIMutI CAGTG 1 cut(s) 339
Cac8I GCNNGC 3 cut(s) 27, 141, 169
CfoI GCGC 1 cut(s) 314
Cfr13I GGNCC 2 cut(s) 48, 289
CviAII CATG 4 cut(s) 136, 182, 205, 209
CviJI RGCY 7 cut(s) 25, 50, 71, 92, 235, 290, 357
CviKI_1 RGCY 7 cut(s) 25, 50, 71, 92, 235, 290, 357
DpnI GATC 3 cut(s) 114, 152, 161
DpnII GATC 3 cut(s) 112, 150, 159
Eam1104I CTCTTC 1 cut(s) 122
EarI CTCTTC 1 cut(s) 122
Eco130I CCWWGG 1 cut(s) 30
Eco147I AGGCCT 2 cut(s) 92, 357
EcoO109I RGGNCCY 1 cut(s) 289
EcoRII CCWGG 1 cut(s) 319
EcoT14I CCWWGG 1 cut(s) 30
EcoT22I ATGCAT 2 cut(s) 6, 210
ErhI CCWWGG 1 cut(s) 30
FaeI CATG 4 cut(s) 139, 185, 208, 212
FaiI YATR 8 cut(s) 6, 137, 183, 195, 206, 210, 266, 277
FatI CATG 4 cut(s) 135, 181, 204, 208
FauI CCCGC 1 cut(s) 182
Fnu4HI GCNGC 1 cut(s) 118
Fsp4HI GCNGC 1 cut(s) 118
GlaI GCGC 1 cut(s) 313
GluI GCNGC 1 cut(s) 118
HaeIII GGCC 4 cut(s) 50, 92, 290, 357
HhaI GCGC 1 cut(s) 314
Hin1II CATG 4 cut(s) 139, 185, 208, 212
Hin6I GCGC 1 cut(s) 312
HinP1I GCGC 1 cut(s) 312
HinfI GANTC 1 cut(s) 36
Hpy188I TCNGA 1 cut(s) 256
Hpy188III TCNNGA 1 cut(s) 64
HpyAV CCTTC 3 cut(s) 82, 214, 347
HpyCH4V TGCA 5 cut(s) 4, 60, 97, 208, 268
Hsp92II CATG 4 cut(s) 139, 185, 208, 212
HspAI GCGC 1 cut(s) 312
Kzo9I GATC 3 cut(s) 112, 150, 159
LpnPI CCDG 8 cut(s) 25, 49, 85, 153, 158, 226, 306, 333
LweI GCATC 1 cut(s) 69
MalI GATC 3 cut(s) 114, 152, 161
MboI GATC 3 cut(s) 112, 150, 159
MboII GAAGA 1 cut(s) 139
MflI RGATCY 1 cut(s) 150
MhlI GDGCHC 1 cut(s) 145
MluCI AATT 3 cut(s) 190, 199, 363
MmeI TCCRAC 1 cut(s) 178
MnlI CCTC 5 cut(s) 39, 72, 302, 351, 368
Mph1103I ATGCAT 2 cut(s) 6, 210
MseI TTAA 1 cut(s) 189
MspR9I CCNGG 1 cut(s) 321
Mva1269I GAATGC 2 cut(s) 177, 314
MvaI CCWGG 1 cut(s) 321
NdeII GATC 3 cut(s) 112, 150, 159
NlaIII CATG 4 cut(s) 139, 185, 208, 212
NlaIV GGNNCC 3 cut(s) 152, 172, 339
NsiI ATGCAT 2 cut(s) 6, 210
NspI RCATGY 1 cut(s) 139
PasI CCCWGGG 1 cut(s) 320
PceI AGGCCT 2 cut(s) 92, 357
PctI GAATGC 2 cut(s) 177, 314
PfeI GAWTC 1 cut(s) 36
PkrI GCNGC 1 cut(s) 119
Psp6I CCWGG 1 cut(s) 319
PspGI CCWGG 1 cut(s) 319
PspN4I GGNNCC 3 cut(s) 152, 172, 339
PspPI GGNCC 2 cut(s) 48, 289
PsuI RGATCY 1 cut(s) 150
SaqAI TTAA 1 cut(s) 189
SatI GCNGC 1 cut(s) 118
Sau3AI GATC 3 cut(s) 112, 150, 159
Sau96I GGNCC 2 cut(s) 48, 289
ScrFI CCNGG 1 cut(s) 321
SduI GDGCHC 1 cut(s) 145
SetI ASST 2 cut(s) 27, 237
SfaNI GCATC 1 cut(s) 69
SfcI CTRYAG 1 cut(s) 106
Sse9I AATT 3 cut(s) 190, 199, 363
SseBI AGGCCT 2 cut(s) 92, 357
SsiI CCGC 2 cut(s) 118, 175
StuI AGGCCT 2 cut(s) 92, 357
StyD4I CCNGG 1 cut(s) 319
StyI CCWWGG 1 cut(s) 30
TasI AATT 3 cut(s) 190, 199, 363
TauI GCSGC 1 cut(s) 120
TfiI GAWTC 1 cut(s) 36
Tru1I TTAA 1 cut(s) 189
Tru9I TTAA 1 cut(s) 189
TscAI CASTG 1 cut(s) 339
TspDTI ATGAA 3 cut(s) 170, 193, 233
TspGWI ACGGA 1 cut(s) 172
TspRI CASTG 1 cut(s) 339
XapI RAATTY 2 cut(s) 199, 363
XceI RCATGY 1 cut(s) 139
XcmI CCANNNNNNNNNTGG 1 cut(s) 79
Zsp2I ATGCAT 2 cut(s) 6, 210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.