pycom10g18230

Binds directly to 23S ribosomal RNA and is necessary for the in vitro assembly process of the 50S ribosomal subunit. It is not involved in the protein synthesizing functions of that subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0006084
Physical Location & Seq
Reverse (-)
21544715 .. 21545017
303 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g18230.1

Sequence Viewer

Length: 303 bp
ATGAATAAGGGTAAGATTTTCAAGCTAGCCAAGGGCTTCAGGGGAAGGGCCAAGAATTGCATAAGGATTGCCAGAGAGAGGGTGGAGAAGGCCTTGCAGTATTCCTACAGGGATCGCCGCACCAAGAAGCGAGACATGCGCTCCCTGTGGATCCAGCGTATCAATGCTGGCACCCGCATCCATGGAGTATGTTTCAAATTGTGCCCCTATTTTATATGTGATGCATTTTTGAGAGAACTGTACAAGTTGCTATTGTCTTTGATATGGTTTTGGATTTTAACCTCAGTTGTTTTACTTTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L20

Protein Analysis

101

Amino Acids

12.0

Weight (kDa)

10.66

Isoelectric Point (pI)

24.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L20 PF00453 3 - 65 6.1e-25 Ribosomal protein L20
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 170
AciI CCGC 2 cut(s) 118, 175
AclWI GGATC 3 cut(s) 120, 145, 158
AcuI CTGAAG 1 cut(s) 22
AfaI GTAC 1 cut(s) 242
AfiI CCNNNNNNNGG 1 cut(s) 78
AgsI TTSAA 2 cut(s) 22, 196
AluBI AGCT 1 cut(s) 25
AluI AGCT 1 cut(s) 25
Alw26I GTCTC 1 cut(s) 126
AlwI GGATC 3 cut(s) 120, 145, 158
AoxI GGCC 2 cut(s) 48, 90
AspLEI GCGC 1 cut(s) 141
AspS9I GGNCC 1 cut(s) 48
AsuNHI GCTAGC 1 cut(s) 25
BaeGI GKGCMC 1 cut(s) 206
BamHI GGATCC 1 cut(s) 150
BanI GGYRCC 1 cut(s) 170
BcoDI GTCTC 1 cut(s) 126
BfaI CTAG 1 cut(s) 26
BfmI CTRYAG 1 cut(s) 106
BisI GCNGC 1 cut(s) 118
BlsI GCNGC 1 cut(s) 119
BmgT120I GGNCC 1 cut(s) 48
BmiI GGNNCC 2 cut(s) 152, 172
BmsI GCATC 2 cut(s) 186, 211
BmtI GCTAGC 1 cut(s) 29
BsaJI CCNNGG 2 cut(s) 30, 181
BsaXI ACNNNNNCTCC 2 cut(s) 125, 155
Bsc4I CCNNNNNNNGG 1 cut(s) 78
BseDI CCNNGG 2 cut(s) 30, 181
BseGI GGATG 1 cut(s) 177
BseLI CCNNNNNNNGG 1 cut(s) 78
BseMII CTCAG 1 cut(s) 297
BseSI GKGCMC 1 cut(s) 206
BshFI GGCC 2 cut(s) 50, 92
BshNI GGYRCC 1 cut(s) 170
BslI CCNNNNNNNGG 1 cut(s) 78
BsmAI GTCTC 1 cut(s) 126
BsnI GGCC 2 cut(s) 50, 92
Bsp1286I GDGCHC 1 cut(s) 206
Bsp1407I TGTACA 1 cut(s) 240
Bsp143I GATC 2 cut(s) 112, 150
Bsp19I CCATGG 1 cut(s) 181
BspACI CCGC 2 cut(s) 118, 175
BspANI GGCC 2 cut(s) 50, 92
BspCNI CTCAG 1 cut(s) 296
BspLI GGNNCC 2 cut(s) 152, 172
BspOI GCTAGC 1 cut(s) 29
BspPI GGATC 3 cut(s) 120, 145, 158
BspT107I GGYRCC 1 cut(s) 170
BsrGI TGTACA 1 cut(s) 240
BssECI CCNNGG 2 cut(s) 30, 181
BssMI GATC 2 cut(s) 112, 150
BssT1I CCWWGG 2 cut(s) 30, 181
Bst4CI ACNGT 1 cut(s) 240
BstAUI TGTACA 1 cut(s) 240
BstC8I GCNNGC 2 cut(s) 27, 169
BstDEI CTNAG 1 cut(s) 283
BstDSI CCRYGG 1 cut(s) 181
BstF5I GGATG 1 cut(s) 177
BstHHI GCGC 1 cut(s) 141
BstKTI GATC 2 cut(s) 115, 153
BstMAI GTCTC 1 cut(s) 126
BstMBI GATC 2 cut(s) 112, 150
BstMWI GCNNNNNNNGC 1 cut(s) 136
BstNSI RCATGY 1 cut(s) 139
BstSFI CTRYAG 1 cut(s) 106
BstSLI GKGCMC 1 cut(s) 206
BstX2I RGATCY 1 cut(s) 150
BstYI RGATCY 1 cut(s) 150
BsuRI GGCC 2 cut(s) 50, 92
BtgI CCRYGG 1 cut(s) 181
BtsCI GGATG 1 cut(s) 177
Cac8I GCNNGC 2 cut(s) 27, 169
CfoI GCGC 1 cut(s) 141
Cfr13I GGNCC 1 cut(s) 48
Csp6I GTAC 1 cut(s) 241
CviAII CATG 2 cut(s) 136, 182
CviJI RGCY 5 cut(s) 25, 29, 36, 50, 92
CviKI_1 RGCY 5 cut(s) 25, 29, 36, 50, 92
CviQI GTAC 1 cut(s) 241
DdeI CTNAG 1 cut(s) 283
DpnI GATC 2 cut(s) 114, 152
DpnII GATC 2 cut(s) 112, 150
Eco130I CCWWGG 2 cut(s) 30, 181
Eco147I AGGCCT 1 cut(s) 92
Eco57I CTGAAG 1 cut(s) 22
EcoT14I CCWWGG 2 cut(s) 30, 181
EcoT22I ATGCAT 1 cut(s) 226
ErhI CCWWGG 2 cut(s) 30, 181
FaeI CATG 2 cut(s) 139, 185
FaiI YATR 8 cut(s) 62, 137, 183, 190, 215, 217, 265, 301
FatI CATG 2 cut(s) 135, 181
FauI CCCGC 1 cut(s) 182
Fnu4HI GCNGC 1 cut(s) 118
FokI GGATG 1 cut(s) 164
Fsp4HI GCNGC 1 cut(s) 118
FspBI CTAG 1 cut(s) 26
GlaI GCGC 1 cut(s) 140
GluI GCNGC 1 cut(s) 118
HaeIII GGCC 2 cut(s) 50, 92
HhaI GCGC 1 cut(s) 141
Hin1II CATG 2 cut(s) 139, 185
Hin6I GCGC 1 cut(s) 139
HinP1I GCGC 1 cut(s) 139
HpyAV CCTTC 2 cut(s) 39, 82
HpyCH4III ACNGT 1 cut(s) 240
HpyCH4V TGCA 3 cut(s) 60, 97, 224
HpyF10VI GCNNNNNNNGC 1 cut(s) 136
HpyF3I CTNAG 1 cut(s) 283
Hsp92II CATG 2 cut(s) 139, 185
HspAI GCGC 1 cut(s) 139
Kzo9I GATC 2 cut(s) 112, 150
LmnI GCTCC 1 cut(s) 146
LpnPI CCDG 6 cut(s) 25, 85, 94, 153, 158, 167
LweI GCATC 2 cut(s) 186, 211
MaeI CTAG 1 cut(s) 26
MalI GATC 2 cut(s) 114, 152
MboI GATC 2 cut(s) 112, 150
MflI RGATCY 1 cut(s) 150
MhlI GDGCHC 1 cut(s) 206
MluCI AATT 2 cut(s) 55, 197
MnlI CCTC 2 cut(s) 72, 292
Mph1103I ATGCAT 1 cut(s) 226
MseI TTAA 1 cut(s) 278
MwoI GCNNNNNNNGC 1 cut(s) 136
NcoI CCATGG 1 cut(s) 181
NdeII GATC 2 cut(s) 112, 150
NheI GCTAGC 1 cut(s) 25
NlaIII CATG 2 cut(s) 139, 185
NlaIV GGNNCC 2 cut(s) 152, 172
NsiI ATGCAT 1 cut(s) 226
NspI RCATGY 1 cut(s) 139
PceI AGGCCT 1 cut(s) 92
PkrI GCNGC 1 cut(s) 119
PspN4I GGNNCC 2 cut(s) 152, 172
PspPI GGNCC 1 cut(s) 48
PsuI RGATCY 1 cut(s) 150
RsaI GTAC 1 cut(s) 242
RsaNI GTAC 1 cut(s) 241
SaqAI TTAA 1 cut(s) 278
SatI GCNGC 1 cut(s) 118
Sau3AI GATC 2 cut(s) 112, 150
Sau96I GGNCC 1 cut(s) 48
SduI GDGCHC 1 cut(s) 206
SetI ASST 2 cut(s) 27, 284
SfaNI GCATC 2 cut(s) 186, 211
SfcI CTRYAG 1 cut(s) 106
Sse9I AATT 2 cut(s) 55, 197
SseBI AGGCCT 1 cut(s) 92
SsiI CCGC 2 cut(s) 118, 175
SspMI CTAG 1 cut(s) 26
StuI AGGCCT 1 cut(s) 92
StyI CCWWGG 2 cut(s) 30, 181
TaaI ACNGT 1 cut(s) 240
TasI AATT 2 cut(s) 55, 197
TatI WGTACW 1 cut(s) 240
TauI GCSGC 1 cut(s) 120
Tru1I TTAA 1 cut(s) 278
Tru9I TTAA 1 cut(s) 278
TspDTI ATGAA 2 cut(s) 17, 288
XceI RCATGY 1 cut(s) 139
XcmI CCANNNNNNNNNTGG 1 cut(s) 79
XspI CTAG 1 cut(s) 26
Zsp2I ATGCAT 1 cut(s) 226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.