Rorug05G0079100

Binds directly to 23S ribosomal RNA and is necessary for the in vitro assembly process of the 50S ribosomal subunit. It is not involved in the protein synthesizing functions of that subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0006084
Physical Location & Seq
Forward (+)
6833885 .. 6838035
4151 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0079100.1

Sequence Viewer

Length: 777 bp
ATGGCCGAGAGAGAGAACCACGTCTACACCGCCAAGCTCGCCGAGCAAGCCGAGCGCTACGACGAAATGGTGGAGGCGATGACGAAAGTGGCGAAGCTTGACGTGGAGCTGACTATCGAGGAGCGGAATCTGCTGTCTGTGGGGTACAAGAACGTGATCGGAGCACGGAGGGCTTCTTGGAGGATACTGTCCTCGATTGAGCAGAAGGAGGAGAGCAAGGGGAATGAGCAGAATGTGAGCCGTATCAAGGAGTACAGGAACAAGGTGGAGACTGAGCTCTCCAGCATTTGCAGTGACATCATGAGGGTTATTGATGAGCATCTCCTTCCGTCTTGCTCGGCTTTCGAGTCCACTGTGTTCTTCTACAAAATGAAAGGAGATTACTATCGCTACTTGGCTGAGTTTAAGACTGGTGATGACAGGAAAGAGGTTGCTGATCAGTCAATGAAGGCTTATCAGGCAGCTTCTAGCAAAGCAGAGACTGATTTACCTCCCACACATCCCATCAGGCTGGGATTGGCCTTGAACTTCTCTGTCTTTTACTATGAAATCTTGAATTCTCCTGAAAGAGCCTGTCATCTTGCAAAGCAAGCCTTTGATGAAGCTATCTCCGAGCTGGATACTTTGAGTGAGGAGTCATACAAGGACAGCACCTTAATCATGCAACTCTTGAGGGACAACCTCACATTGTGGACCTCTGACATTCCGGAGGATGGAGTTGAAGAAGGGCAGAAGGCGGACAGCTCTGCAAAAACTGGAGGTGAAGATGCAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L20

Protein Analysis

258

Amino Acids

29.34

Weight (kDa)

4.78

Isoelectric Point (pI)

47.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
14-3-3 PF00244 10 - 232 5.7e-108 14-3-3 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 124
AccI GTMKAC 1 cut(s) 24
AccIII TCCGGA 1 cut(s) 706
AciI CCGC 3 cut(s) 30, 124, 737
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 556
AdeI CACNNNGTG 1 cut(s) 690
AfaI GTAC 2 cut(s) 146, 254
AfeI AGCGCT 1 cut(s) 56
AfiI CCNNNNNNNGG 2 cut(s) 247, 713
AgsI TTSAA 3 cut(s) 526, 556, 722
AjiI CACGTC 2 cut(s) 22, 103
AluBI AGCT 8 cut(s) 37, 97, 109, 277, 464, 605, 616, 744
AluI AGCT 8 cut(s) 37, 97, 109, 277, 464, 605, 616, 744
Alw21I GWGCWC 2 cut(s) 166, 279
Alw26I GTCTC 2 cut(s) 263, 473
AlwNI CAGNNNCTG 1 cut(s) 482
Aor13HI TCCGGA 1 cut(s) 706
Aor51HI AGCGCT 1 cut(s) 56
AoxI GGCC 2 cut(s) 3, 519
ApeKI GCWGC 1 cut(s) 461
ApoI RAATTY 1 cut(s) 556
AspLEI GCGC 1 cut(s) 57
AspS9I GGNCC 1 cut(s) 693
AsuHPI GGTGA 2 cut(s) 425, 773
AvaII GGWCC 1 cut(s) 693
BanII GRGCYC 1 cut(s) 279
Bbv12I GWGCWC 2 cut(s) 166, 279
BbvI GCAGC 1 cut(s) 473
BccI CCATC 2 cut(s) 512, 707
BceAI ACGGC 1 cut(s) 225
BciVI GTATCC 2 cut(s) 177, 613
BclI TGATCA 1 cut(s) 436
BcoDI GTCTC 2 cut(s) 263, 473
BfaI CTAG 1 cut(s) 468
BfoI RGCGCY 1 cut(s) 58
BfuI GTATCC 2 cut(s) 177, 613
BisI GCNGC 1 cut(s) 462
BlsI GCNGC 1 cut(s) 463
Bme18I GGWCC 1 cut(s) 693
BmgBI CACGTC 2 cut(s) 22, 103
BmgT120I GGNCC 1 cut(s) 693
BmsI GCATC 2 cut(s) 328, 757
BpmI CTGGAG 2 cut(s) 265, 777
BpuEI CTTGAG 1 cut(s) 691
BsaBI GATNNNNATC 2 cut(s) 318, 384
BsaWI WCCGGW 1 cut(s) 706
Bsc4I CCNNNNNNNGG 2 cut(s) 247, 713
Bse1I ACTGG 2 cut(s) 415, 760
Bse8I GATNNNNATC 2 cut(s) 318, 384
BseAI TCCGGA 1 cut(s) 706
BseGI GGATG 2 cut(s) 499, 718
BseJI GATNNNNATC 2 cut(s) 318, 384
BseLI CCNNNNNNNGG 2 cut(s) 247, 713
BseMII CTCAG 2 cut(s) 264, 390
BseNI ACTGG 2 cut(s) 415, 760
BseRI GAGGAG 3 cut(s) 134, 224, 647
BseXI GCAGC 1 cut(s) 473
BseYI CCCAGC 1 cut(s) 511
BshFI GGCC 2 cut(s) 5, 521
BsiHKAI GWGCWC 2 cut(s) 166, 279
BsiSI CCGG 1 cut(s) 707
BslFI GGGAC 1 cut(s) 689
BslI CCNNNNNNNGG 2 cut(s) 247, 713
BsmAI GTCTC 2 cut(s) 263, 473
BsmFI GGGAC 1 cut(s) 689
BsnI GGCC 2 cut(s) 5, 521
Bsp1286I GDGCHC 2 cut(s) 166, 279
Bsp13I TCCGGA 1 cut(s) 706
Bsp143I GATC 2 cut(s) 156, 436
BspACI CCGC 3 cut(s) 30, 124, 737
BspANI GGCC 2 cut(s) 5, 521
BspCNI CTCAG 2 cut(s) 265, 391
BspEI TCCGGA 1 cut(s) 706
BspHI TCATGA 1 cut(s) 300
BsrBI CCGCTC 1 cut(s) 124
BsrI ACTGG 2 cut(s) 415, 760
BssMI GATC 2 cut(s) 156, 436
Bst4CI ACNGT 2 cut(s) 189, 355
BstC8I GCNNGC 3 cut(s) 39, 48, 591
BstDEI CTNAG 2 cut(s) 273, 399
BstF5I GGATG 2 cut(s) 499, 718
BstH2I RGCGCY 1 cut(s) 58
BstHHI GCGC 1 cut(s) 57
BstKTI GATC 2 cut(s) 159, 439
BstMAI GTCTC 2 cut(s) 263, 473
BstMBI GATC 2 cut(s) 156, 436
BstMWI GCNNNNNNNGC 8 cut(s) 38, 43, 47, 52, 130, 170, 458, 590
BstV1I GCAGC 1 cut(s) 473
BstXI CCANNNNNNTGG 1 cut(s) 511
BsuI GTATCC 2 cut(s) 177, 613
BsuRI GGCC 2 cut(s) 5, 521
BtgZI GCGATG 1 cut(s) 92
BtrI CACGTC 2 cut(s) 22, 103
BtsCI GGATG 2 cut(s) 499, 718
BtsI GCAGTG 1 cut(s) 298
BtsIMutI CAGTG 2 cut(s) 298, 351
Cac8I GCNNGC 3 cut(s) 39, 48, 591
CaiI CAGNNNCTG 1 cut(s) 482
CciI TCATGA 1 cut(s) 300
CfoI GCGC 1 cut(s) 57
Cfr13I GGNCC 1 cut(s) 693
Csp6I GTAC 2 cut(s) 145, 253
CviAII CATG 2 cut(s) 301, 661
CviQI GTAC 2 cut(s) 145, 253
DdeI CTNAG 2 cut(s) 273, 399
DpnI GATC 2 cut(s) 158, 438
DpnII GATC 2 cut(s) 156, 436
DraIII CACNNNGTG 1 cut(s) 690
EaeI YGGCCR 1 cut(s) 3
EciI GGCGGA 1 cut(s) 752
Ecl136II GAGCTC 1 cut(s) 277
Eco24I GRGCYC 1 cut(s) 279
Eco47I GGWCC 1 cut(s) 693
Eco47III AGCGCT 1 cut(s) 56
Eco53kI GAGCTC 1 cut(s) 277
EcoICRI GAGCTC 1 cut(s) 277
EcoRI GAATTC 1 cut(s) 556
EcoT38I GRGCYC 1 cut(s) 279
FaeI CATG 2 cut(s) 304, 664
FaiI YATR 4 cut(s) 302, 546, 640, 662
FalI AAGNNNNNCTT 2 cut(s) 578, 610
FaqI GGGAC 1 cut(s) 689
FatI CATG 2 cut(s) 300, 660
FbaI TGATCA 1 cut(s) 436
FblI GTMKAC 1 cut(s) 24
Fnu4HI GCNGC 1 cut(s) 462
FokI GGATG 2 cut(s) 486, 725
FriOI GRGCYC 1 cut(s) 279
Fsp4HI GCNGC 1 cut(s) 462
FspBI CTAG 1 cut(s) 468
GlaI GCGC 1 cut(s) 56
GluI GCNGC 1 cut(s) 462
GsaI CCCAGC 1 cut(s) 515
GsuI CTGGAG 2 cut(s) 265, 777
HaeII RGCGCY 1 cut(s) 58
HaeIII GGCC 2 cut(s) 5, 521
HapII CCGG 1 cut(s) 707
HhaI GCGC 1 cut(s) 57
Hin1II CATG 2 cut(s) 304, 664
Hin6I GCGC 1 cut(s) 55
HinP1I GCGC 1 cut(s) 55
HindIII AAGCTT 1 cut(s) 95
HinfI GANTC 3 cut(s) 127, 347, 635
HpaII CCGG 1 cut(s) 707
HphI GGTGA 2 cut(s) 425, 773
Hpy166II GTNNAC 3 cut(s) 25, 351, 693
Hpy188I TCNGA 3 cut(s) 161, 613, 700
Hpy188III TCNNGA 5 cut(s) 301, 553, 563, 670, 707
Hpy8I GTNNAC 3 cut(s) 25, 351, 693
Hpy99I CGWCG 1 cut(s) 65
HpyAV CCTTC 5 cut(s) 199, 335, 442, 719, 727
HpyCH4III ACNGT 2 cut(s) 189, 355
HpyCH4IV ACGT 3 cut(s) 21, 102, 153
HpyCH4V TGCA 5 cut(s) 291, 584, 664, 749, 770
HpyF10VI GCNNNNNNNGC 8 cut(s) 38, 43, 47, 52, 130, 170, 458, 590
HpyF3I CTNAG 2 cut(s) 273, 399
HpySE526I ACGT 3 cut(s) 21, 102, 153
Hsp92II CATG 2 cut(s) 304, 664
HspAI GCGC 1 cut(s) 55
Kpn2I TCCGGA 1 cut(s) 706
Ksp22I TGATCA 1 cut(s) 436
Kzo9I GATC 2 cut(s) 156, 436
LmnI GCTCC 3 cut(s) 106, 121, 161
Lsp1109I GCAGC 1 cut(s) 473
LweI GCATC 2 cut(s) 328, 757
MaeI CTAG 1 cut(s) 468
MaeII ACGT 3 cut(s) 21, 102, 153
MaeIII GTNAC 1 cut(s) 293
MalI GATC 2 cut(s) 158, 438
MbiI CCGCTC 1 cut(s) 124
MboI GATC 2 cut(s) 156, 436
MboII GAAGA 3 cut(s) 352, 734, 776
MhlI GDGCHC 2 cut(s) 166, 279
MluCI AATT 1 cut(s) 556
MlyI GAGTC 2 cut(s) 356, 644
MroI TCCGGA 1 cut(s) 706
MseI TTAA 2 cut(s) 405, 656
MspI CCGG 1 cut(s) 707
MwoI GCNNNNNNNGC 8 cut(s) 38, 43, 47, 52, 130, 170, 458, 590
NdeII GATC 2 cut(s) 156, 436
NlaIII CATG 2 cut(s) 304, 664
NmeAIII GCCGAG 4 cut(s) 31, 67, 76, 317
NmuCI GTSAC 1 cut(s) 293
PagI TCATGA 1 cut(s) 300
PcsI WCGNNNNNNNCGW 1 cut(s) 89
PfeI GAWTC 1 cut(s) 127
PkrI GCNGC 1 cut(s) 463
PleI GAGTC 2 cut(s) 355, 643
PpsI GAGTC 2 cut(s) 355, 643
Psp124BI GAGCTC 1 cut(s) 279
PspFI CCCAGC 1 cut(s) 511
PspPI GGNCC 1 cut(s) 693
PsrI GAACNNNNNNTAC 2 cut(s) 8, 40
PstNI CAGNNNCTG 1 cut(s) 482
RsaI GTAC 2 cut(s) 146, 254
RsaNI GTAC 2 cut(s) 145, 253
SacI GAGCTC 1 cut(s) 279
SaqAI TTAA 2 cut(s) 405, 656
SatI GCNGC 1 cut(s) 462
Sau3AI GATC 2 cut(s) 156, 436
Sau96I GGNCC 1 cut(s) 693
SchI GAGTC 2 cut(s) 356, 644
SduI GDGCHC 2 cut(s) 166, 279
SfaNI GCATC 2 cut(s) 328, 757
SinI GGWCC 1 cut(s) 693
SmlI CTYRAG 1 cut(s) 670
SmoI CTYRAG 1 cut(s) 670
Sse9I AATT 1 cut(s) 556
SsiI CCGC 3 cut(s) 30, 124, 737
SspMI CTAG 1 cut(s) 468
SstI GAGCTC 1 cut(s) 279
TaaI ACNGT 2 cut(s) 189, 355
TaiI ACGT 3 cut(s) 24, 105, 156
TaqI TCGA 3 cut(s) 117, 194, 345
TasI AATT 1 cut(s) 556
TatI WGTACW 1 cut(s) 252
TfiI GAWTC 1 cut(s) 127
Tru1I TTAA 2 cut(s) 405, 656
Tru9I TTAA 2 cut(s) 405, 656
TscAI CASTG 2 cut(s) 298, 358
TseFI GTSAC 1 cut(s) 293
TseI GCWGC 1 cut(s) 461
Tsp45I GTSAC 1 cut(s) 293
TspDTI ATGAA 4 cut(s) 386, 461, 561, 615
TspGWI ACGGA 2 cut(s) 181, 318
TspRI CASTG 2 cut(s) 298, 358
VpaK11BI GGWCC 1 cut(s) 693
XapI RAATTY 1 cut(s) 556
XmiI GTMKAC 1 cut(s) 24
XspI CTAG 1 cut(s) 468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.