AT1G20575

dolichol-phosphate mannosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0016586
Physical Location & Seq
Reverse (-)
7126747 .. 7128762
2016 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G20575.1

Sequence Viewer

Length: 741 bp
ATGGCGGACGAGATGGAGACCAAAGGAGAGAAGAAGTACAAGTATAGCATAATCATTCCTACCTACAACGAGCGCCTCAACATCGCTATCATAGTCTACCTGATTTTCAAGCATCTCCGGGACGTTGATTTTGAGATAATTGTTGTGGATGATGGGAGTCCTGATGGCACACAGGAAATTGTCAAGCAACTGCAGCAATTGTATGGTGAAGACCGTATCCTTTTAAGAGCTAGAGCCAAGAAGCTTGGTTTGGGAACTGCATATATTCATGGTTTGAAGCATGCTACGGGTGATTTCGTCGTGATCATGGATGCTGATCTTTCACATCATCCAAAATACTTACCAAGTTTCATCAAGAAACAACTAGAGACAAATGCAAGTATAGTAACTGGTACACGATATGTAAAAGGTGGTGGTGTACACGGGTGGAATCTTATGCGGAAACTCACTAGTAGAGGAGCAAATGTGCTAGCTCAAACACTTTTATGGCCTGGTGTATCTGATTTAACAGGATCATTCCGGCTATACAAGAAATCGGCGCTTGAAGACGTGATTAGCTCATGTGTGAGTAAAGGGTATGTCTTTCAGATGGAAATGATTGTTCGTGCAACCAGAAAAGGATACCATATTGAAGAGGTACCGATCACTTTTGTTGACAGAGTCTTTGGAACTTCGAAACTCGGAGGATCTGAAATAGTGGAATATCTAAAAGGCCTCGTCTATCTCCTGCTTACAACTTAA

Protein Analysis

246

Amino Acids

27.68

Weight (kDa)

9.07

Isoelectric Point (pI)

21.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_tranf_2_3 PF13641 15 - 225 1.7e-10 Glycosyltransferase like family 2
Glycos_transf_2 PF00535 16 - 184 8.7e-40 Glycosyl transferase family 2
Glyco_tranf_2_2 PF10111 16 - 119 2.1e-08 Glycosyltransferase like family 2
Glyco_transf_21 PF13506 88 - 184 1.1e-06 Glycosyl transferase family 21
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016586)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20575
fragaria_vesca FvH4_5g10320
malus_domestica MD06G1125000.v1.1
prunus_persica Prupe.5G135600_v2.0.a1
pyrus_communis pycom06g11560
rosa_chinensis RchiOBHm_Chr7g0187901
rosa_laevigata RLG00000004749
rosa_multiflora Rmu_sc0022114.1_g000004
rosa_roxburghii Rroxscaffold_3G00266960
rosa_rugosa Rorug06G0485300
rosa_samantha Rh7AG090400 Rh7BG092000 Rh7CG091100
rosa_wichuraiana Rw7G007800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 637
AccB1I GGYRCC 1 cut(s) 637
AccI GTMKAC 1 cut(s) 96
AciI CCGC 2 cut(s) 5, 439
AclWI GGATC 2 cut(s) 520, 694
AfaI GTAC 4 cut(s) 38, 394, 420, 639
AgsI TTSAA 4 cut(s) 109, 277, 545, 632
AhlI ACTAGT 1 cut(s) 449
AjiI CACGTC 1 cut(s) 550
AjnI CCWGG 1 cut(s) 490
AjuI GAANNNNNNNTTGG 2 cut(s) 233, 265
AluBI AGCT 4 cut(s) 230, 244, 473, 558
AluI AGCT 4 cut(s) 230, 244, 473, 558
Alw26I GTCTC 2 cut(s) 11, 362
AlwI GGATC 2 cut(s) 520, 694
AoxI GGCC 2 cut(s) 488, 712
ApeKI GCWGC 1 cut(s) 193
ArsI GACNNNNNNTTYG 4 cut(s) 113, 145, 647, 679
Asp718I GGTACC 1 cut(s) 637
AspLEI GCGC 2 cut(s) 75, 541
AsuC2I CCSGG 1 cut(s) 119
AsuHPI GGTGA 2 cut(s) 218, 302
AsuII TTCGAA 1 cut(s) 674
AsuNHI GCTAGC 1 cut(s) 469
BanI GGYRCC 1 cut(s) 637
BbsI GAAGAC 2 cut(s) 216, 552
BbvI GCAGC 1 cut(s) 205
BccI CCATC 4 cut(s) 7, 146, 158, 583
BciT130I CCWGG 1 cut(s) 492
BciVI GTATCC 2 cut(s) 227, 614
BclI TGATCA 1 cut(s) 303
BcnI CCSGG 1 cut(s) 119
BcoDI GTCTC 2 cut(s) 11, 362
BcuI ACTAGT 1 cut(s) 449
BfaI CTAG 4 cut(s) 231, 365, 450, 470
BfmI CTRYAG 1 cut(s) 191
BfoI RGCGCY 2 cut(s) 76, 542
BfuI GTATCC 2 cut(s) 227, 614
BisI GCNGC 1 cut(s) 194
BlsI GCNGC 1 cut(s) 195
Bme1390I CCNGG 2 cut(s) 119, 492
BmgBI CACGTC 1 cut(s) 550
BmiI GGNNCC 1 cut(s) 639
BmrFI CCNGG 2 cut(s) 119, 492
BmsI GCATC 2 cut(s) 121, 301
BmtI GCTAGC 1 cut(s) 473
BpiI GAAGAC 2 cut(s) 216, 552
Bpu14I TTCGAA 1 cut(s) 674
BpuMI CCSGG 1 cut(s) 119
BsaBI GATNNNNATC 1 cut(s) 315
BsaI GGTCTC 1 cut(s) 11
BsaXI ACNNNNNCTCC 2 cut(s) 450, 480
Bse1I ACTGG 1 cut(s) 394
Bse8I GATNNNNATC 1 cut(s) 315
BseBI CCWGG 1 cut(s) 492
BseGI GGATG 3 cut(s) 154, 316, 328
BseJI GATNNNNATC 1 cut(s) 315
BseNI ACTGG 1 cut(s) 394
BseRI GAGGAG 1 cut(s) 471
BseXI GCAGC 1 cut(s) 205
BshFI GGCC 2 cut(s) 490, 714
BshNI GGYRCC 1 cut(s) 637
BsiSI CCGG 2 cut(s) 118, 520
BslFI GGGAC 1 cut(s) 134
BsmAI GTCTC 2 cut(s) 11, 362
BsmFI GGGAC 1 cut(s) 134
BsnI GGCC 2 cut(s) 490, 714
Bso31I GGTCTC 1 cut(s) 11
Bsp119I TTCGAA 1 cut(s) 674
Bsp1407I TGTACA 1 cut(s) 418
Bsp143I GATC 5 cut(s) 303, 316, 512, 642, 686
BspACI CCGC 2 cut(s) 5, 439
BspANI GGCC 2 cut(s) 490, 714
BspLI GGNNCC 1 cut(s) 639
BspMAI CTGCAG 1 cut(s) 195
BspOI GCTAGC 1 cut(s) 473
BspPI GGATC 2 cut(s) 520, 694
BspT104I TTCGAA 1 cut(s) 674
BspT107I GGYRCC 1 cut(s) 637
BspTNI GGTCTC 1 cut(s) 11
BsrGI TGTACA 1 cut(s) 418
BsrI ACTGG 1 cut(s) 394
BssMI GATC 5 cut(s) 303, 316, 512, 642, 686
Bst2UI CCWGG 1 cut(s) 492
Bst4CI ACNGT 1 cut(s) 215
Bst6I CTCTTC 1 cut(s) 627
BstAUI TGTACA 1 cut(s) 418
BstBI TTCGAA 1 cut(s) 674
BstC8I GCNNGC 2 cut(s) 282, 471
BstF5I GGATG 3 cut(s) 154, 316, 328
BstH2I RGCGCY 2 cut(s) 76, 542
BstHHI GCGC 2 cut(s) 75, 541
BstKTI GATC 5 cut(s) 306, 319, 515, 645, 689
BstMAI GTCTC 2 cut(s) 11, 362
BstMBI GATC 5 cut(s) 303, 316, 512, 642, 686
BstMWI GCNNNNNNNGC 1 cut(s) 193
BstNI CCWGG 1 cut(s) 492
BstNSI RCATGY 1 cut(s) 284
BstSCI CCNGG 2 cut(s) 117, 490
BstSFI CTRYAG 1 cut(s) 191
BstV1I GCAGC 1 cut(s) 205
BstV2I GAAGAC 2 cut(s) 216, 552
BstX2I RGATCY 1 cut(s) 686
BstYI RGATCY 1 cut(s) 686
BsuI GTATCC 2 cut(s) 227, 614
BsuRI GGCC 2 cut(s) 490, 714
BtgZI GCGATG 1 cut(s) 67
BtrI CACGTC 1 cut(s) 550
BtsCI GGATG 3 cut(s) 154, 316, 328
Cac8I GCNNGC 2 cut(s) 282, 471
CfoI GCGC 2 cut(s) 75, 541
Csp6I GTAC 4 cut(s) 37, 393, 419, 638
CviAII CATG 4 cut(s) 269, 281, 307, 561
CviJI RGCY 8 cut(s) 230, 236, 244, 473, 490, 523, 558, 714
CviKI_1 RGCY 8 cut(s) 230, 236, 244, 473, 490, 523, 558, 714
CviQI GTAC 4 cut(s) 37, 393, 419, 638
DpnI GATC 5 cut(s) 305, 318, 514, 644, 688
DpnII GATC 5 cut(s) 303, 316, 512, 642, 686
Eam1104I CTCTTC 1 cut(s) 627
EarI CTCTTC 1 cut(s) 627
EciI GGCGGA 1 cut(s) 20
Eco147I AGGCCT 1 cut(s) 714
Eco31I GGTCTC 1 cut(s) 11
EcoRII CCWGG 1 cut(s) 490
FaeI CATG 4 cut(s) 272, 284, 310, 564
FaqI GGGAC 1 cut(s) 134
FatI CATG 4 cut(s) 268, 280, 306, 560
FbaI TGATCA 1 cut(s) 303
FblI GTMKAC 1 cut(s) 96
Fnu4HI GCNGC 1 cut(s) 194
FokI GGATG 3 cut(s) 161, 315, 323
Fsp4HI GCNGC 1 cut(s) 194
FspBI CTAG 4 cut(s) 231, 365, 450, 470
GlaI GCGC 2 cut(s) 74, 540
GluI GCNGC 1 cut(s) 194
HaeII RGCGCY 2 cut(s) 76, 542
HaeIII GGCC 2 cut(s) 490, 714
HapII CCGG 2 cut(s) 118, 520
HhaI GCGC 2 cut(s) 75, 541
Hin1II CATG 4 cut(s) 272, 284, 310, 564
Hin6I GCGC 2 cut(s) 73, 539
HinP1I GCGC 2 cut(s) 73, 539
HincII GTYRAC 1 cut(s) 655
HindII GTYRAC 1 cut(s) 655
HindIII AAGCTT 1 cut(s) 242
HinfI GANTC 3 cut(s) 157, 430, 660
HpaII CCGG 2 cut(s) 118, 520
HphI GGTGA 2 cut(s) 218, 302
Hpy166II GTNNAC 5 cut(s) 97, 395, 419, 421, 655
Hpy188I TCNGA 4 cut(s) 502, 588, 683, 691
Hpy188III TCNNGA 3 cut(s) 161, 301, 355
Hpy8I GTNNAC 5 cut(s) 97, 395, 419, 421, 655
Hpy99I CGWCG 1 cut(s) 302
HpyCH4III ACNGT 1 cut(s) 215
HpyCH4IV ACGT 2 cut(s) 123, 549
HpyCH4V TGCA 4 cut(s) 193, 260, 377, 608
HpyF10VI GCNNNNNNNGC 1 cut(s) 193
HpySE526I ACGT 2 cut(s) 123, 549
Hsp92II CATG 4 cut(s) 272, 284, 310, 564
HspAI GCGC 2 cut(s) 73, 539
KpnI GGTACC 1 cut(s) 641
Ksp22I TGATCA 1 cut(s) 303
Kzo9I GATC 5 cut(s) 303, 316, 512, 642, 686
LmnI GCTCC 1 cut(s) 458
Lsp1109I GCAGC 1 cut(s) 205
LweI GCATC 2 cut(s) 121, 301
MaeI CTAG 4 cut(s) 231, 365, 450, 470
MaeII ACGT 2 cut(s) 123, 549
MaeIII GTNAC 1 cut(s) 385
MalI GATC 5 cut(s) 305, 318, 514, 644, 688
MboI GATC 5 cut(s) 303, 316, 512, 642, 686
MboII GAAGA 4 cut(s) 43, 221, 557, 644
MfeI CAATTG 1 cut(s) 197
MflI RGATCY 1 cut(s) 686
MluCI AATT 3 cut(s) 138, 177, 197
MlyI GAGTC 2 cut(s) 166, 669
MnlI CCTC 5 cut(s) 86, 449, 628, 677, 725
MseI TTAA 3 cut(s) 224, 506, 739
MslI CAYNNNNRTG 1 cut(s) 484
MspI CCGG 2 cut(s) 118, 520
MspR9I CCNGG 2 cut(s) 119, 492
MunI CAATTG 1 cut(s) 197
MvaI CCWGG 1 cut(s) 492
MwoI GCNNNNNNNGC 1 cut(s) 193
NciI CCSGG 1 cut(s) 119
NdeII GATC 5 cut(s) 303, 316, 512, 642, 686
NheI GCTAGC 1 cut(s) 469
NlaIII CATG 4 cut(s) 272, 284, 310, 564
NlaIV GGNNCC 1 cut(s) 639
NspI RCATGY 1 cut(s) 284
NspV TTCGAA 1 cut(s) 674
PaeI GCATGC 1 cut(s) 284
PceI AGGCCT 1 cut(s) 714
PfeI GAWTC 1 cut(s) 430
PflFI GACNNNGTC 1 cut(s) 659
PfoI TCCNGGA 1 cut(s) 117
PkrI GCNGC 1 cut(s) 195
PleI GAGTC 2 cut(s) 165, 668
PpsI GAGTC 2 cut(s) 165, 668
Psp6I CCWGG 1 cut(s) 490
PspGI CCWGG 1 cut(s) 490
PspN4I GGNNCC 1 cut(s) 639
PstI CTGCAG 1 cut(s) 195
PsuI RGATCY 1 cut(s) 686
PsyI GACNNNGTC 1 cut(s) 659
RsaI GTAC 4 cut(s) 38, 394, 420, 639
RsaNI GTAC 4 cut(s) 37, 393, 419, 638
RseI CAYNNNNRTG 1 cut(s) 484
SaqAI TTAA 3 cut(s) 224, 506, 739
SatI GCNGC 1 cut(s) 194
Sau3AI GATC 5 cut(s) 303, 316, 512, 642, 686
SchI GAGTC 2 cut(s) 166, 669
ScrFI CCNGG 2 cut(s) 119, 492
SfaNI GCATC 2 cut(s) 121, 301
SfcI CTRYAG 1 cut(s) 191
SfuI TTCGAA 1 cut(s) 674
SmiMI CAYNNNNRTG 1 cut(s) 484
SpeI ACTAGT 1 cut(s) 449
SphI GCATGC 1 cut(s) 284
Sse9I AATT 3 cut(s) 138, 177, 197
SseBI AGGCCT 1 cut(s) 714
SsiI CCGC 2 cut(s) 5, 439
SspMI CTAG 4 cut(s) 231, 365, 450, 470
StuI AGGCCT 1 cut(s) 714
StyD4I CCNGG 2 cut(s) 117, 490
TaaI ACNGT 1 cut(s) 215
TaiI ACGT 2 cut(s) 126, 552
TaqI TCGA 1 cut(s) 674
TasI AATT 3 cut(s) 138, 177, 197
TatI WGTACW 2 cut(s) 36, 418
TfiI GAWTC 1 cut(s) 430
Tru1I TTAA 3 cut(s) 224, 506, 739
Tru9I TTAA 3 cut(s) 224, 506, 739
TseI GCWGC 1 cut(s) 193
TspDTI ATGAA 2 cut(s) 257, 340
Tth111I GACNNNGTC 1 cut(s) 659
XceI RCATGY 1 cut(s) 284
XmiI GTMKAC 1 cut(s) 96
XspI CTAG 4 cut(s) 231, 365, 450, 470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.