MD06G1125000.v1.1

Dolichol-phosphate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0016586
Physical Location & Seq
Reverse (-)
26704481 .. 26707546
3066 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1125000.v1.1.491

Sequence Viewer

Length: 717 bp
ATGGAGACGAAGAACAAGTACAGCATCATAGTCCCGACCTACAACGAGCGCCTCAACATCGCCCTCCTCGTCTACCTCGTCTTCAAGCATCTCCGGGATGTTGACTTTGAGATAATCGTCGTTGACGATGGAAGCCCCGACGGCACTCAGGAGATTGTTGAACAGTTGCAGCAAGTCTACGGTCACGATCGCATCCTGTTGAGAGCTAGAGCTAAGAAGCTTGGGTTAGGAACGGCTTATATTCATGGTTTGAAGCATGCTTCCGGGAACTTTGTTGTGATCATGGACGCAGATTTATCACATCACCCAAAGTATTTGCCAAGCTTCATCAAGAAACAGCTGGAAACTGGTGCAAGTATAGTTACTGGAACTCGTTATGTCAGAGGTGGCGGGGTCCATGGGTGGAATCTTATGCGCAAACTAACAAGTAGAGGAGCCAATGTTCTTGCACAAACACTACTATGGCCTGGTGTATCAGATTTGACTGGATCATTTCGGCTTTACAGGAAAACAGTGCTTGAAGATATCATTAGTTCCTGTGTCAGCAAGGGCTACGTCTTTCAGATGGAGATGATAGTTCGTGCTTCTAGAAAAGGATACCATATCGAAGAGGTTCCAATCAGTTTTGTTGACAGGGTATATGGAATTTCAAAGCTCGGAGGATCTGAAATTGTCGAATATCTGAAAGGCCTCGTCTATCTTCTCGTTACAACATGA

Protein Analysis

239

Amino Acids

26.73

Weight (kDa)

9.26

Isoelectric Point (pI)

21.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_tranf_2_3 PF13641 7 - 213 3.3e-11 Glycosyltransferase like family 2
Glycos_transf_2 PF00535 8 - 176 7.7e-39 Glycosyl transferase family 2
Glyco_tranf_2_2 PF10111 8 - 101 3e-07 Glycosyltransferase like family 2
Glyco_transf_21 PF13506 80 - 176 8.2e-06 Glycosyl transferase family 21
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016586)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20575
fragaria_vesca FvH4_5g10320
malus_domestica MD06G1125000.v1.1
prunus_persica Prupe.5G135600_v2.0.a1
pyrus_communis pycom06g11560
rosa_chinensis RchiOBHm_Chr7g0187901
rosa_laevigata RLG00000004749
rosa_multiflora Rmu_sc0022114.1_g000004
rosa_roxburghii Rroxscaffold_3G00266960
rosa_rugosa Rorug06G0485300
rosa_samantha Rh7AG090400 Rh7BG092000 Rh7CG091100
rosa_wichuraiana Rw7G007800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 416
AccI GTMKAC 2 cut(s) 72, 177
AciI CCGC 1 cut(s) 390
AclWI GGATC 2 cut(s) 496, 670
AcsI RAATTY 1 cut(s) 645
AfaI GTAC 1 cut(s) 20
AgsI TTSAA 5 cut(s) 85, 161, 253, 521, 651
AjnI CCWGG 1 cut(s) 466
AloI GAACNNNNNNTCC 2 cut(s) 426, 458
AluBI AGCT 6 cut(s) 206, 212, 220, 324, 340, 655
AluI AGCT 6 cut(s) 206, 212, 220, 324, 340, 655
AlwI GGATC 2 cut(s) 496, 670
AoxI GGCC 2 cut(s) 464, 688
ApeKI GCWGC 1 cut(s) 169
ApoI RAATTY 1 cut(s) 645
Asp700I GAANNNNTTC 1 cut(s) 612
AspLEI GCGC 2 cut(s) 51, 417
AspS9I GGNCC 1 cut(s) 394
AsuC2I CCSGG 2 cut(s) 95, 265
AsuHPI GGTGA 1 cut(s) 296
AvaII GGWCC 1 cut(s) 394
BarI GAAGNNNNNNTAC 1 cut(s) 34
BbsI GAAGAC 1 cut(s) 73
BbvI GCAGC 1 cut(s) 181
BccI CCATC 2 cut(s) 122, 559
BceAI ACGGC 2 cut(s) 157, 249
BciT130I CCWGG 1 cut(s) 468
BciVI GTATCC 1 cut(s) 590
BclI TGATCA 1 cut(s) 279
BcnI CCSGG 2 cut(s) 95, 265
BfaI CTAG 2 cut(s) 207, 588
BfoI RGCGCY 1 cut(s) 52
BfuI GTATCC 1 cut(s) 590
BglI GCCNNNNNGGC 1 cut(s) 141
BisI GCNGC 1 cut(s) 170
BlsI GCNGC 1 cut(s) 171
Bme1390I CCNGG 3 cut(s) 95, 265, 468
Bme18I GGWCC 1 cut(s) 394
BmgT120I GGNCC 1 cut(s) 394
BmiI GGNNCC 3 cut(s) 395, 436, 615
BmrFI CCNGG 3 cut(s) 95, 265, 468
BmsI GCATC 3 cut(s) 33, 97, 201
BpiI GAAGAC 1 cut(s) 73
BpuMI CCSGG 2 cut(s) 95, 265
BsaJI CCNNGG 1 cut(s) 397
BsaXI ACNNNNNCTCC 2 cut(s) 426, 456
Bse1I ACTGG 3 cut(s) 352, 370, 490
BseBI CCWGG 1 cut(s) 468
BseDI CCNNGG 1 cut(s) 397
BseGI GGATG 2 cut(s) 103, 192
BseMII CTCAG 1 cut(s) 161
BseNI ACTGG 3 cut(s) 352, 370, 490
BseRI GAGGAG 2 cut(s) 56, 447
BseXI GCAGC 1 cut(s) 181
Bsh1285I CGRYCG 1 cut(s) 190
BshFI GGCC 2 cut(s) 466, 690
BsiEI CGRYCG 1 cut(s) 190
BsiSI CCGG 2 cut(s) 94, 264
BslFI GGGAC 1 cut(s) 17
BsmFI GGGAC 1 cut(s) 17
BsnI GGCC 2 cut(s) 466, 690
Bsp143I GATC 4 cut(s) 187, 279, 488, 662
Bsp19I CCATGG 1 cut(s) 397
BspACI CCGC 1 cut(s) 390
BspANI GGCC 2 cut(s) 466, 690
BspCNI CTCAG 1 cut(s) 160
BspLI GGNNCC 3 cut(s) 395, 436, 615
BspPI GGATC 2 cut(s) 496, 670
BsrI ACTGG 3 cut(s) 352, 370, 490
BssECI CCNNGG 1 cut(s) 397
BssMI GATC 4 cut(s) 187, 279, 488, 662
BssT1I CCWWGG 1 cut(s) 397
Bst2UI CCWGG 1 cut(s) 468
Bst4CI ACNGT 3 cut(s) 165, 182, 514
Bst6I CTCTTC 1 cut(s) 603
BstC8I GCNNGC 1 cut(s) 258
BstDEI CTNAG 2 cut(s) 147, 213
BstDSI CCRYGG 1 cut(s) 397
BstF5I GGATG 2 cut(s) 103, 192
BstH2I RGCGCY 1 cut(s) 52
BstHHI GCGC 2 cut(s) 51, 417
BstKTI GATC 4 cut(s) 190, 282, 491, 665
BstMBI GATC 4 cut(s) 187, 279, 488, 662
BstMCI CGRYCG 1 cut(s) 190
BstMWI GCNNNNNNNGC 1 cut(s) 141
BstNI CCWGG 1 cut(s) 468
BstNSI RCATGY 1 cut(s) 260
BstSCI CCNGG 3 cut(s) 93, 263, 466
BstV1I GCAGC 1 cut(s) 181
BstV2I GAAGAC 1 cut(s) 73
BstX2I RGATCY 1 cut(s) 662
BstYI RGATCY 1 cut(s) 662
BsuI GTATCC 1 cut(s) 590
BsuRI GGCC 2 cut(s) 466, 690
BtgI CCRYGG 1 cut(s) 397
BtgZI GCGATG 1 cut(s) 43
BtsCI GGATG 2 cut(s) 103, 192
BtsIMutI CAGTG 1 cut(s) 519
Cac8I GCNNGC 1 cut(s) 258
CfoI GCGC 2 cut(s) 51, 417
Cfr13I GGNCC 1 cut(s) 394
CseI GACGC 1 cut(s) 296
Csp6I GTAC 1 cut(s) 19
CviAII CATG 5 cut(s) 245, 257, 283, 398, 714
CviQI GTAC 1 cut(s) 19
DdeI CTNAG 2 cut(s) 147, 213
DpnI GATC 4 cut(s) 189, 281, 490, 664
DpnII GATC 4 cut(s) 187, 279, 488, 662
Eam1104I CTCTTC 1 cut(s) 603
EarI CTCTTC 1 cut(s) 603
Eco130I CCWWGG 1 cut(s) 397
Eco147I AGGCCT 1 cut(s) 690
Eco32I GATATC 1 cut(s) 526
Eco47I GGWCC 1 cut(s) 394
EcoRII CCWGG 1 cut(s) 466
EcoRV GATATC 1 cut(s) 526
EcoT14I CCWWGG 1 cut(s) 397
ErhI CCWWGG 1 cut(s) 397
FaeI CATG 5 cut(s) 248, 260, 286, 401, 717
FaqI GGGAC 1 cut(s) 17
FatI CATG 5 cut(s) 244, 256, 282, 397, 713
FauI CCCGC 1 cut(s) 383
FbaI TGATCA 1 cut(s) 279
FblI GTMKAC 2 cut(s) 72, 177
Fnu4HI GCNGC 1 cut(s) 170
FokI GGATG 2 cut(s) 110, 179
Fsp4HI GCNGC 1 cut(s) 170
FspBI CTAG 2 cut(s) 207, 588
FspI TGCGCA 1 cut(s) 416
GlaI GCGC 2 cut(s) 50, 416
GluI GCNGC 1 cut(s) 170
HaeII RGCGCY 1 cut(s) 52
HaeIII GGCC 2 cut(s) 466, 690
HapII CCGG 2 cut(s) 94, 264
HgaI GACGC 1 cut(s) 296
HhaI GCGC 2 cut(s) 51, 417
Hin1II CATG 5 cut(s) 248, 260, 286, 401, 717
Hin6I GCGC 2 cut(s) 49, 415
HinP1I GCGC 2 cut(s) 49, 415
HincII GTYRAC 3 cut(s) 103, 124, 631
HindII GTYRAC 3 cut(s) 103, 124, 631
HindIII AAGCTT 2 cut(s) 218, 322
HinfI GANTC 1 cut(s) 406
HpaII CCGG 2 cut(s) 94, 264
HphI GGTGA 1 cut(s) 296
Hpy166II GTNNAC 5 cut(s) 73, 103, 124, 178, 631
Hpy188I TCNGA 6 cut(s) 383, 478, 564, 659, 667, 684
Hpy188III TCNNGA 5 cut(s) 34, 149, 185, 331, 588
Hpy8I GTNNAC 5 cut(s) 73, 103, 124, 178, 631
Hpy99I CGWCG 2 cut(s) 122, 143
HpyCH4III ACNGT 3 cut(s) 165, 182, 514
HpyCH4IV ACGT 1 cut(s) 555
HpyCH4V TGCA 3 cut(s) 169, 353, 449
HpyF10VI GCNNNNNNNGC 1 cut(s) 141
HpyF3I CTNAG 2 cut(s) 147, 213
HpySE526I ACGT 1 cut(s) 555
Hsp92II CATG 5 cut(s) 248, 260, 286, 401, 717
HspAI GCGC 2 cut(s) 49, 415
Ksp22I TGATCA 1 cut(s) 279
Kzo9I GATC 4 cut(s) 187, 279, 488, 662
LmnI GCTCC 1 cut(s) 434
Lsp1109I GCAGC 1 cut(s) 181
LweI GCATC 3 cut(s) 33, 97, 201
MaeI CTAG 2 cut(s) 207, 588
MaeII ACGT 1 cut(s) 555
MaeIII GTNAC 3 cut(s) 182, 361, 706
MalI GATC 4 cut(s) 189, 281, 490, 664
MboI GATC 4 cut(s) 187, 279, 488, 662
MboII GAAGA 5 cut(s) 22, 73, 533, 620, 692
MflI RGATCY 1 cut(s) 662
MluCI AATT 2 cut(s) 645, 669
MnlI CCTC 9 cut(s) 62, 74, 77, 86, 377, 425, 604, 653, 701
MroXI GAANNNNTTC 1 cut(s) 612
MslI CAYNNNNRTG 1 cut(s) 460
MspA1I CMGCKG 1 cut(s) 340
MspI CCGG 2 cut(s) 94, 264
MspR9I CCNGG 3 cut(s) 95, 265, 468
MvaI CCWGG 1 cut(s) 468
MwoI GCNNNNNNNGC 1 cut(s) 141
NciI CCSGG 2 cut(s) 95, 265
NcoI CCATGG 1 cut(s) 397
NdeII GATC 4 cut(s) 187, 279, 488, 662
NlaIII CATG 5 cut(s) 248, 260, 286, 401, 717
NlaIV GGNNCC 3 cut(s) 395, 436, 615
NmuCI GTSAC 1 cut(s) 182
NsbI TGCGCA 1 cut(s) 416
NspI RCATGY 1 cut(s) 260
PaeI GCATGC 1 cut(s) 260
PceI AGGCCT 1 cut(s) 690
PcsI WCGNNNNNNNCGW 3 cut(s) 66, 75, 123
PdmI GAANNNNTTC 1 cut(s) 612
PfeI GAWTC 1 cut(s) 406
PfoI TCCNGGA 2 cut(s) 93, 263
PkrI GCNGC 1 cut(s) 171
Ple19I CGATCG 1 cut(s) 190
Psp6I CCWGG 1 cut(s) 466
PspGI CCWGG 1 cut(s) 466
PspN4I GGNNCC 3 cut(s) 395, 436, 615
PspPI GGNCC 1 cut(s) 394
PsuI RGATCY 1 cut(s) 662
PvuI CGATCG 1 cut(s) 190
PvuII CAGCTG 1 cut(s) 340
RsaI GTAC 1 cut(s) 20
RsaNI GTAC 1 cut(s) 19
RseI CAYNNNNRTG 1 cut(s) 460
SatI GCNGC 1 cut(s) 170
Sau3AI GATC 4 cut(s) 187, 279, 488, 662
Sau96I GGNCC 1 cut(s) 394
ScrFI CCNGG 3 cut(s) 95, 265, 468
SfaNI GCATC 3 cut(s) 33, 97, 201
SinI GGWCC 1 cut(s) 394
SmiMI CAYNNNNRTG 1 cut(s) 460
SphI GCATGC 1 cut(s) 260
Sse9I AATT 2 cut(s) 645, 669
SseBI AGGCCT 1 cut(s) 690
SsiI CCGC 1 cut(s) 390
SspMI CTAG 2 cut(s) 207, 588
StuI AGGCCT 1 cut(s) 690
StyD4I CCNGG 3 cut(s) 93, 263, 466
StyI CCWWGG 1 cut(s) 397
TaaI ACNGT 3 cut(s) 165, 182, 514
TaiI ACGT 1 cut(s) 558
TaqI TCGA 2 cut(s) 606, 675
TasI AATT 2 cut(s) 645, 669
TatI WGTACW 1 cut(s) 18
TfiI GAWTC 1 cut(s) 406
TscAI CASTG 1 cut(s) 519
TseFI GTSAC 1 cut(s) 182
TseI GCWGC 1 cut(s) 169
Tsp45I GTSAC 1 cut(s) 182
TspDTI ATGAA 2 cut(s) 233, 316
TspRI CASTG 1 cut(s) 519
VpaK11BI GGWCC 1 cut(s) 394
XapI RAATTY 1 cut(s) 645
XbaI TCTAGA 1 cut(s) 587
XceI RCATGY 1 cut(s) 260
XmiI GTMKAC 2 cut(s) 72, 177
XmnI GAANNNNTTC 1 cut(s) 612
XspI CTAG 2 cut(s) 207, 588
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.