Rorug06G0485300

Dolichol-phosphate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0015303
Physical Location & Seq
Forward (+)
60494129 .. 60496330
2202 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0485300.1

Sequence Viewer

Length: 990 bp
ATGAGCACGCTGAAGTTGGATTCGATTTCTCCTACTGAAACCGTGGAGATAGAGAATGGTCTGTCACTGGTGCCGCGGCTCCGGCTGAATATAACGGTGTATCCGTCGAGCCAAGTTGTGAGCAAACCGATTGACGAGTGGAAGATGAAGCAAACCCTAATTGACTTCCTCAAGAACTCGCTTTCCGATCCGGTCACAGTTCCGGAGGAGGATCTCAAGATCAGACGGGTGAGGGACCTGAAGAAGCGGAAGCGGGACGACCCTGTAGCTTCCGGTACCATCTTCATTCGCGACCTAGGGTTTCTGAGGAAGACGAGTAGGAAGGGTGAGGAGGTGGAGAAGAAGTTCAGTGATTGGAGGAGGTACATTGTGGAGAAGATGGATGGGATTGAGCTCAATCTCGAAGGGGTTAAGTTCAAGCTAAACGTCGCCGTTCCGGAGTCGGATGATTTCCAGGGGATGAAGAAAGGTTGGGAGGAGTTTTACGCTTTCGGAAATCGAGGGTTTTCGAGGGGTGGGAAGCAAGAGACTGATACTATAATACTGAGGGGTGTTCCTTCACGCTGGTTCGCAGAGCCACGGGTTTCGTCTAAGCCTTCAATGCTGGTTACACATACCATCTTTTCGACATTTGGGAAGATAAGGAATCTTAATGTTGCTGAGGACGATGATCCAGGTAAGGATGCGAATGAGGATGATGGGGACTTGGTTTCAGGTCTCCATTGTAAGATTGTGGTTCAGTTCGAGAAACACAAGGACTTCTGCAACACTTTCAAGGCGTTTTGTGGCCGCTCATTGCAAAAGGAAGGATCTAAGTTGAAGGCTGATTATGAGGTTGCTTGGGACAAGGATGGATTTTTCCGAAACTCGAGAAATCAAACACAGGAAGACAGTAGCAGGGCACGAGAAACCACAACATATCCCAGGCAGCAGCAACACTATTCTCGGTTCAGTTCCGATGAAGCTCCCAGAAAGAGGTTTAAGGAATAG

Protein Analysis

329

Amino Acids

37.97

Weight (kDa)

9.0

Isoelectric Point (pI)

50.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RBD_AKAP-17A PF25015 113 - 297 2.3e-32 AKAP-17A-like, RNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015303)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G63310
fragaria_vesca FvH4_5g10310
malus_domestica MD00G1118700.v1.1 MD06G1125100.v1.1
prunus_persica Prupe.5G135700_v2.0.a1
pyrus_communis pycom14g11720
rosa_chinensis RchiOBHm_Chr7g0187911
rosa_laevigata RLG00000004748
rosa_multiflora Rmu_co8094926.1_g000001
rosa_roxburghii Rroxscaffold_3G00266950
rosa_rugosa Rorug06G0485300
rosa_samantha Rh7AG090500 Rh7BG092100 Rh7CG091200 Rh7DG092900
rosa_wichuraiana Rw7G007810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 275
AccB1I GGYRCC 2 cut(s) 70, 275
AccBSI CCGCTC 1 cut(s) 792
AccII CGCG 2 cut(s) 76, 291
AccIII TCCGGA 2 cut(s) 202, 436
AciI CCGC 5 cut(s) 74, 76, 247, 253, 790
AclWI GGATC 4 cut(s) 182, 219, 665, 817
AcoI YGGCCR 1 cut(s) 787
AcuI CTGAAG 2 cut(s) 32, 260
AfaI GTAC 2 cut(s) 277, 365
AfiI CCNNNNNNNGG 1 cut(s) 975
AgsI TTSAA 4 cut(s) 418, 600, 775, 820
AjnI CCWGG 3 cut(s) 453, 673, 923
AloI GAACNNNNNNTCC 4 cut(s) 167, 199, 329, 361
AluBI AGCT 4 cut(s) 269, 394, 421, 965
AluI AGCT 4 cut(s) 269, 394, 421, 965
Alw21I GWGCWC 2 cut(s) 8, 396
Alw26I GTCTC 2 cut(s) 521, 722
AlwI GGATC 4 cut(s) 182, 219, 665, 817
Ama87I CYCGRG 1 cut(s) 868
Aor13HI TCCGGA 2 cut(s) 202, 436
AoxI GGCC 1 cut(s) 787
ApeKI GCWGC 2 cut(s) 928, 931
Asp700I GAANNNNTTC 1 cut(s) 344
Asp718I GGTACC 1 cut(s) 275
AspA2I CCTAGG 1 cut(s) 295
AspS9I GGNCC 1 cut(s) 235
AsuHPI GGTGA 2 cut(s) 241, 338
AvaI CYCGRG 1 cut(s) 868
AvaII GGWCC 1 cut(s) 235
AvrII CCTAGG 1 cut(s) 295
BaeGI GKGCMC 1 cut(s) 904
BanI GGYRCC 2 cut(s) 70, 275
BanII GRGCYC 1 cut(s) 396
BauI CACGAG 1 cut(s) 903
BbsI GAAGAC 2 cut(s) 317, 894
Bbv12I GWGCWC 2 cut(s) 8, 396
BbvCI CCTCAGC 1 cut(s) 660
BbvI GCAGC 2 cut(s) 940, 943
BccI CCATC 6 cut(s) 287, 373, 377, 626, 692, 845
BceAI ACGGC 1 cut(s) 416
BciT130I CCWGG 3 cut(s) 455, 675, 925
BciVI GTATCC 1 cut(s) 111
BcoDI GTCTC 2 cut(s) 521, 722
BfaI CTAG 1 cut(s) 296
BfmI CTRYAG 1 cut(s) 264
BfuI GTATCC 1 cut(s) 111
BisI GCNGC 5 cut(s) 74, 77, 790, 929, 932
BlnI CCTAGG 1 cut(s) 295
BlsI GCNGC 5 cut(s) 75, 78, 791, 930, 933
Bme1390I CCNGG 3 cut(s) 455, 675, 925
Bme18I GGWCC 1 cut(s) 235
BmeT110I CYCGRG 1 cut(s) 868
BmgT120I GGNCC 1 cut(s) 235
BmiI GGNNCC 4 cut(s) 72, 80, 236, 277
BmrFI CCNGG 3 cut(s) 455, 675, 925
BmsI GCATC 1 cut(s) 673
BpiI GAAGAC 2 cut(s) 317, 894
Bpu10I CCTNAGC 1 cut(s) 660
BpuEI CTTGAG 2 cut(s) 155, 200
BsaI GGTCTC 1 cut(s) 722
BsaJI CCNNGG 6 cut(s) 42, 74, 295, 454, 578, 923
BsaWI WCCGGW 4 cut(s) 190, 202, 272, 436
BsaXI ACNNNNNCTCC 2 cut(s) 329, 359
Bsc4I CCNNNNNNNGG 1 cut(s) 975
Bse1I ACTGG 1 cut(s) 72
Bse3DI GCAATG 1 cut(s) 794
BseAI TCCGGA 2 cut(s) 202, 436
BseBI CCWGG 3 cut(s) 455, 675, 925
BseDI CCNNGG 6 cut(s) 42, 74, 295, 454, 578, 923
BseGI GGATG 6 cut(s) 388, 451, 465, 688, 700, 856
BseLI CCNNNNNNNGG 1 cut(s) 975
BseMI GCAATG 1 cut(s) 794
BseMII CTCAG 3 cut(s) 296, 536, 651
BseNI ACTGG 1 cut(s) 72
BseRI GAGGAG 4 cut(s) 221, 344, 373, 491
BseSI GKGCMC 1 cut(s) 904
BseXI GCAGC 2 cut(s) 940, 943
Bsh1236I CGCG 2 cut(s) 76, 291
BshFI GGCC 1 cut(s) 789
BshNI GGYRCC 2 cut(s) 70, 275
BsiHKAI GWGCWC 2 cut(s) 8, 396
BsiHKCI CYCGRG 1 cut(s) 868
BsiSI CCGG 5 cut(s) 82, 191, 203, 273, 437
BslFI GGGAC 4 cut(s) 248, 269, 716, 857
BslI CCNNNNNNNGG 1 cut(s) 975
BsmAI GTCTC 2 cut(s) 521, 722
BsmFI GGGAC 4 cut(s) 248, 269, 716, 857
BsnI GGCC 1 cut(s) 789
Bso31I GGTCTC 1 cut(s) 722
BsoBI CYCGRG 1 cut(s) 868
Bsp1286I GDGCHC 3 cut(s) 8, 396, 904
Bsp13I TCCGGA 2 cut(s) 202, 436
Bsp143I GATC 5 cut(s) 187, 211, 219, 670, 809
Bsp68I TCGCGA 1 cut(s) 291
BspACI CCGC 5 cut(s) 74, 76, 247, 253, 790
BspANI GGCC 1 cut(s) 789
BspCNI CTCAG 3 cut(s) 297, 537, 652
BspEI TCCGGA 2 cut(s) 202, 436
BspFNI CGCG 2 cut(s) 76, 291
BspLI GGNNCC 4 cut(s) 72, 80, 236, 277
BspPI GGATC 4 cut(s) 182, 219, 665, 817
BspT107I GGYRCC 2 cut(s) 70, 275
BspTNI GGTCTC 1 cut(s) 722
BsrBI CCGCTC 1 cut(s) 792
BsrDI GCAATG 1 cut(s) 794
BsrI ACTGG 1 cut(s) 72
BssECI CCNNGG 6 cut(s) 42, 74, 295, 454, 578, 923
BssMI GATC 5 cut(s) 187, 211, 219, 670, 809
BssSI CACGAG 1 cut(s) 903
BssT1I CCWWGG 1 cut(s) 295
Bst2BI CACGAG 1 cut(s) 903
Bst2UI CCWGG 3 cut(s) 455, 675, 925
Bst4CI ACNGT 4 cut(s) 43, 97, 199, 893
BstC8I GCNNGC 1 cut(s) 8
BstDEI CTNAG 5 cut(s) 305, 545, 591, 660, 813
BstDSI CCRYGG 3 cut(s) 42, 74, 578
BstF5I GGATG 6 cut(s) 388, 451, 465, 688, 700, 856
BstFNI CGCG 2 cut(s) 76, 291
BstKTI GATC 5 cut(s) 190, 214, 222, 673, 812
BstMAI GTCTC 2 cut(s) 521, 722
BstMBI GATC 5 cut(s) 187, 211, 219, 670, 809
BstMWI GCNNNNNNNGC 2 cut(s) 82, 601
BstNI CCWGG 3 cut(s) 455, 675, 925
BstSCI CCNGG 3 cut(s) 453, 673, 923
BstSFI CTRYAG 1 cut(s) 264
BstSLI GKGCMC 1 cut(s) 904
BstUI CGCG 2 cut(s) 76, 291
BstV1I GCAGC 2 cut(s) 940, 943
BstV2I GAAGAC 2 cut(s) 317, 894
BstX2I RGATCY 2 cut(s) 211, 809
BstYI RGATCY 2 cut(s) 211, 809
BsuI GTATCC 1 cut(s) 111
BsuRI GGCC 1 cut(s) 789
BtgI CCRYGG 3 cut(s) 42, 74, 578
BtsCI GGATG 6 cut(s) 388, 451, 465, 688, 700, 856
BtsIMutI CAGTG 2 cut(s) 65, 355
BtuMI TCGCGA 1 cut(s) 291
Cac8I GCNNGC 1 cut(s) 8
Cfr13I GGNCC 1 cut(s) 235
Cfr42I CCGCGG 1 cut(s) 77
Csp6I GTAC 2 cut(s) 276, 364
CviQI GTAC 2 cut(s) 276, 364
DdeI CTNAG 5 cut(s) 305, 545, 591, 660, 813
DpnI GATC 5 cut(s) 189, 213, 221, 672, 811
DpnII GATC 5 cut(s) 187, 211, 219, 670, 809
EaeI YGGCCR 1 cut(s) 787
Ecl136II GAGCTC 1 cut(s) 394
Eco130I CCWWGG 1 cut(s) 295
Eco24I GRGCYC 1 cut(s) 396
Eco31I GGTCTC 1 cut(s) 722
Eco47I GGWCC 1 cut(s) 235
Eco53kI GAGCTC 1 cut(s) 394
Eco57I CTGAAG 2 cut(s) 32, 260
Eco88I CYCGRG 1 cut(s) 868
EcoICRI GAGCTC 1 cut(s) 394
EcoO109I RGGNCCY 1 cut(s) 235
EcoRII CCWGG 3 cut(s) 453, 673, 923
EcoT14I CCWWGG 1 cut(s) 295
EcoT38I GRGCYC 1 cut(s) 396
ErhI CCWWGG 1 cut(s) 295
FaiI YATR 5 cut(s) 92, 539, 615, 831, 919
FaqI GGGAC 4 cut(s) 248, 269, 716, 857
FauI CCCGC 1 cut(s) 246
Fnu4HI GCNGC 5 cut(s) 74, 77, 790, 929, 932
FokI GGATG 6 cut(s) 395, 458, 472, 695, 707, 863
FriOI GRGCYC 1 cut(s) 396
Fsp4HI GCNGC 5 cut(s) 74, 77, 790, 929, 932
FspBI CTAG 1 cut(s) 296
GluI GCNGC 5 cut(s) 74, 77, 790, 929, 932
HaeIII GGCC 1 cut(s) 789
HapII CCGG 5 cut(s) 82, 191, 203, 273, 437
HinfI GANTC 3 cut(s) 20, 440, 646
HpaII CCGG 5 cut(s) 82, 191, 203, 273, 437
HphI GGTGA 2 cut(s) 241, 338
Hpy188I TCNGA 7 cut(s) 187, 224, 306, 445, 494, 863, 958
Hpy188III TCNNGA 8 cut(s) 172, 203, 217, 290, 401, 437, 745, 870
Hpy99I CGWCG 2 cut(s) 109, 431
HpyAV CCTTC 6 cut(s) 316, 398, 567, 606, 800, 814
HpyCH4III ACNGT 4 cut(s) 43, 97, 199, 893
HpyCH4IV ACGT 1 cut(s) 426
HpyCH4V TGCA 2 cut(s) 765, 799
HpyF10VI GCNNNNNNNGC 2 cut(s) 82, 601
HpyF3I CTNAG 5 cut(s) 305, 545, 591, 660, 813
HpySE526I ACGT 1 cut(s) 426
Kpn2I TCCGGA 2 cut(s) 202, 436
KpnI GGTACC 1 cut(s) 279
KspI CCGCGG 1 cut(s) 77
Kzo9I GATC 5 cut(s) 187, 211, 219, 670, 809
LmnI GCTCC 2 cut(s) 84, 970
Lsp1109I GCAGC 2 cut(s) 940, 943
LweI GCATC 1 cut(s) 673
MaeI CTAG 1 cut(s) 296
MaeII ACGT 1 cut(s) 426
MaeIII GTNAC 3 cut(s) 63, 193, 607
MalI GATC 5 cut(s) 189, 213, 221, 672, 811
MbiI CCGCTC 1 cut(s) 792
MboI GATC 5 cut(s) 187, 211, 219, 670, 809
MboII GAAGA 9 cut(s) 154, 253, 274, 322, 352, 388, 475, 649, 899
MflI RGATCY 2 cut(s) 211, 809
MhlI GDGCHC 3 cut(s) 8, 396, 904
MluCI AATT 1 cut(s) 159
MlyI GAGTC 1 cut(s) 449
MmeI TCCRAC 1 cut(s) 423
MroI TCCGGA 2 cut(s) 202, 436
MroXI GAANNNNTTC 1 cut(s) 344
MseI TTAA 3 cut(s) 411, 651, 981
MspA1I CMGCKG 1 cut(s) 76
MspI CCGG 5 cut(s) 82, 191, 203, 273, 437
MspR9I CCNGG 3 cut(s) 455, 675, 925
MvaI CCWGG 3 cut(s) 455, 675, 925
MvnI CGCG 2 cut(s) 76, 291
MwoI GCNNNNNNNGC 2 cut(s) 82, 601
NdeII GATC 5 cut(s) 187, 211, 219, 670, 809
NlaIV GGNNCC 4 cut(s) 72, 80, 236, 277
NmuCI GTSAC 2 cut(s) 63, 193
NruI TCGCGA 1 cut(s) 291
PaeR7I CTCGAG 1 cut(s) 868
PcsI WCGNNNNNNNCGW 1 cut(s) 101
PdmI GAANNNNTTC 1 cut(s) 344
PfeI GAWTC 2 cut(s) 20, 646
PkrI GCNGC 5 cut(s) 75, 78, 791, 930, 933
PleI GAGTC 1 cut(s) 448
PpsI GAGTC 1 cut(s) 448
PpuMI RGGWCCY 1 cut(s) 235
Psp124BI GAGCTC 1 cut(s) 396
Psp5II RGGWCCY 1 cut(s) 235
Psp6I CCWGG 3 cut(s) 453, 673, 923
PspGI CCWGG 3 cut(s) 453, 673, 923
PspN4I GGNNCC 4 cut(s) 72, 80, 236, 277
PspPI GGNCC 1 cut(s) 235
PspPPI RGGWCCY 1 cut(s) 235
PsuI RGATCY 2 cut(s) 211, 809
RruI TCGCGA 1 cut(s) 291
RsaI GTAC 2 cut(s) 277, 365
RsaNI GTAC 2 cut(s) 276, 364
SacI GAGCTC 1 cut(s) 396
SacII CCGCGG 1 cut(s) 77
SaqAI TTAA 3 cut(s) 411, 651, 981
SatI GCNGC 5 cut(s) 74, 77, 790, 929, 932
Sau3AI GATC 5 cut(s) 187, 211, 219, 670, 809
Sau96I GGNCC 1 cut(s) 235
SchI GAGTC 1 cut(s) 449
ScrFI CCNGG 3 cut(s) 455, 675, 925
SduI GDGCHC 3 cut(s) 8, 396, 904
SfaNI GCATC 1 cut(s) 673
SfcI CTRYAG 1 cut(s) 264
Sfr274I CTCGAG 1 cut(s) 868
Sfr303I CCGCGG 1 cut(s) 77
SgrBI CCGCGG 1 cut(s) 77
SinI GGWCC 1 cut(s) 235
SlaI CTCGAG 1 cut(s) 868
SmlI CTYRAG 3 cut(s) 170, 215, 868
SmoI CTYRAG 3 cut(s) 170, 215, 868
Sse9I AATT 1 cut(s) 159
SsiI CCGC 5 cut(s) 74, 76, 247, 253, 790
SspMI CTAG 1 cut(s) 296
SstI GAGCTC 1 cut(s) 396
StyD4I CCNGG 3 cut(s) 453, 673, 923
StyI CCWWGG 1 cut(s) 295
TaaI ACNGT 4 cut(s) 43, 97, 199, 893
TaiI ACGT 1 cut(s) 429
TaqI TCGA 8 cut(s) 23, 107, 402, 499, 509, 626, 744, 869
TasI AATT 1 cut(s) 159
TauI GCSGC 3 cut(s) 76, 79, 792
TfiI GAWTC 2 cut(s) 20, 646
Tru1I TTAA 3 cut(s) 411, 651, 981
Tru9I TTAA 3 cut(s) 411, 651, 981
TscAI CASTG 2 cut(s) 72, 355
TseFI GTSAC 2 cut(s) 63, 193
TseI GCWGC 2 cut(s) 928, 931
Tsp45I GTSAC 2 cut(s) 63, 193
TspDTI ATGAA 4 cut(s) 161, 274, 476, 975
TspGWI ACGGA 1 cut(s) 93
TspRI CASTG 2 cut(s) 72, 355
VpaK11BI GGWCC 1 cut(s) 235
XhoI CTCGAG 1 cut(s) 868
XmaJI CCTAGG 1 cut(s) 295
XmnI GAANNNNTTC 1 cut(s) 344
XspI CTAG 1 cut(s) 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.