MD00G1118700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0015303
Physical Location & Seq
Forward (+)
25410129 .. 25410668
540 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1118700.v1.1.491

Sequence Viewer

Length: 540 bp
ATGACAAACGAGTCAGTTAAACCCAGCAGCCACTCCTCAACCGATCACGGAGGCAGCAGGAACGACAAGCAACTGGACAAAGAAATCGGAGACATGGTCTCCGCCATCACCAACCGAGTCACGGACATCCACAAGTCTGGCTCCATCCACCACGAGGACGAGGACGAAGATGGCGTCAGGATCATCACCCTTGCAGGATCCAACACCGGAGCTACATTGAGGGGTGAGCTAGATCATGAGAACGTTAACAAACTTGCAGGGCTTCATGGTGGCGGTCCGGCTTTGGAAGAGGCTGATGGGCTGAGCACTTATGTGAACAGCAACTTCCAGGCCGTGAACAACTCCCTCATGATGGGCGGCAACTACACCACGAATGACCCCGGCGTTCGCATGGACATCACAGATGTTGTGGAGCCGCACGGCGGGCATTGTGGGAAGCCGGAGAAGCGGGGGTGGAAGGAGAAGAAGAAGGAGAAGGAGAAGGAGAAGGAGAAGGAGAGGGAGAGCTTCCATAGCGACCAGCATCATGATACGGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

180

Amino Acids

19.61

Weight (kDa)

5.41

Isoelectric Point (pI)

24.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015303)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G63310
fragaria_vesca FvH4_5g10310
malus_domestica MD00G1118700.v1.1 MD06G1125100.v1.1
prunus_persica Prupe.5G135700_v2.0.a1
pyrus_communis pycom14g11720
rosa_chinensis RchiOBHm_Chr7g0187911
rosa_laevigata RLG00000004748
rosa_multiflora Rmu_co8094926.1_g000001
rosa_roxburghii Rroxscaffold_3G00266950
rosa_rugosa Rorug06G0485300
rosa_samantha Rh7AG090500 Rh7BG092100 Rh7CG091200 Rh7DG092900
rosa_wichuraiana Rw7G007810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 10
AciI CCGC 6 cut(s) 102, 273, 357, 416, 423, 448
AclI AACGTT 1 cut(s) 243
AclWI GGATC 3 cut(s) 188, 192, 205
AcyI GRCGYC 1 cut(s) 174
AfiI CCNNNNNNNGG 4 cut(s) 121, 154, 352, 422
AjnI CCWGG 1 cut(s) 327
AleI CACNNNNGTG 1 cut(s) 311
AluBI AGCT 3 cut(s) 212, 229, 507
AluI AGCT 3 cut(s) 212, 229, 507
Alw21I GWGCWC 1 cut(s) 308
Alw26I GTCTC 2 cut(s) 84, 103
AlwI GGATC 3 cut(s) 188, 192, 205
AoxI GGCC 1 cut(s) 330
ApeKI GCWGC 2 cut(s) 27, 54
ArsI GACNNNNNNTTYG 2 cut(s) 159, 191
AspS9I GGNCC 1 cut(s) 275
AsuC2I CCSGG 1 cut(s) 381
AsuHPI GGTGA 3 cut(s) 100, 178, 236
AvaII GGWCC 1 cut(s) 275
BamHI GGATCC 1 cut(s) 197
BauI CACGAG 1 cut(s) 152
Bbv12I GWGCWC 1 cut(s) 308
BbvI GCAGC 2 cut(s) 39, 66
BccI CCATC 5 cut(s) 113, 152, 164, 290, 346
BceAI ACGGC 2 cut(s) 317, 436
BciT130I CCWGG 1 cut(s) 329
BcnI CCSGG 1 cut(s) 381
BcoDI GTCTC 2 cut(s) 84, 103
BfaI CTAG 1 cut(s) 230
BisI GCNGC 4 cut(s) 28, 55, 358, 416
BlpI GCTNAGC 1 cut(s) 302
BlsI GCNGC 4 cut(s) 29, 56, 359, 417
Bme1390I CCNGG 2 cut(s) 329, 381
Bme18I GGWCC 1 cut(s) 275
BmgT120I GGNCC 1 cut(s) 275
BmiI GGNNCC 3 cut(s) 142, 199, 414
BmrFI CCNGG 2 cut(s) 329, 381
BmsI GCATC 1 cut(s) 532
Bpu1102I GCTNAGC 1 cut(s) 302
BpuMI CCSGG 1 cut(s) 381
BsaHI GRCGYC 1 cut(s) 174
BsaI GGTCTC 1 cut(s) 103
BsaJI CCNNGG 1 cut(s) 379
BsaWI WCCGGW 1 cut(s) 206
BsaXI ACNNNNNCTCC 4 cut(s) 81, 83, 111, 113
Bsc4I CCNNNNNNNGG 4 cut(s) 121, 154, 352, 422
Bse1I ACTGG 1 cut(s) 78
BseBI CCWGG 1 cut(s) 329
BseDI CCNNGG 1 cut(s) 379
BseGI GGATG 2 cut(s) 126, 144
BseLI CCNNNNNNNGG 4 cut(s) 121, 154, 352, 422
BseMII CTCAG 1 cut(s) 293
BseNI ACTGG 1 cut(s) 78
BseRI GAGGAG 1 cut(s) 25
BseXI GCAGC 2 cut(s) 39, 66
BseYI CCCAGC 1 cut(s) 23
BshFI GGCC 1 cut(s) 332
BsiHKAI GWGCWC 1 cut(s) 308
BsiSI CCGG 4 cut(s) 207, 278, 381, 440
BslI CCNNNNNNNGG 4 cut(s) 121, 154, 352, 422
BsmAI GTCTC 2 cut(s) 84, 103
BsnI GGCC 1 cut(s) 332
Bso31I GGTCTC 1 cut(s) 103
Bsp1286I GDGCHC 1 cut(s) 308
Bsp143I GATC 4 cut(s) 43, 180, 197, 232
Bsp1720I GCTNAGC 1 cut(s) 302
BspACI CCGC 6 cut(s) 102, 273, 357, 416, 423, 448
BspANI GGCC 1 cut(s) 332
BspCNI CTCAG 1 cut(s) 294
BspHI TCATGA 3 cut(s) 235, 348, 526
BspLI GGNNCC 3 cut(s) 142, 199, 414
BspPI GGATC 3 cut(s) 188, 192, 205
BspTNI GGTCTC 1 cut(s) 103
BsrI ACTGG 1 cut(s) 78
BssECI CCNNGG 1 cut(s) 379
BssMI GATC 4 cut(s) 43, 180, 197, 232
BssNI GRCGYC 1 cut(s) 174
BssSI CACGAG 1 cut(s) 152
Bst2BI CACGAG 1 cut(s) 152
Bst2UI CCWGG 1 cut(s) 329
Bst6I CTCTTC 1 cut(s) 282
BstACI GRCGYC 1 cut(s) 174
BstC8I GCNNGC 1 cut(s) 425
BstDEI CTNAG 1 cut(s) 302
BstF5I GGATG 2 cut(s) 126, 144
BstKTI GATC 4 cut(s) 46, 183, 200, 235
BstMAI GTCTC 2 cut(s) 84, 103
BstMBI GATC 4 cut(s) 43, 180, 197, 232
BstMWI GCNNNNNNNGC 3 cut(s) 424, 445, 513
BstNI CCWGG 1 cut(s) 329
BstSCI CCNGG 2 cut(s) 327, 379
BstV1I GCAGC 2 cut(s) 39, 66
BstX2I RGATCY 1 cut(s) 197
BstXI CCANNNNNNTGG 1 cut(s) 137
BstYI RGATCY 1 cut(s) 197
BsuRI GGCC 1 cut(s) 332
BtsCI GGATG 2 cut(s) 126, 144
Cac8I GCNNGC 1 cut(s) 425
CciI TCATGA 3 cut(s) 235, 348, 526
Cfr13I GGNCC 1 cut(s) 275
CpoI CGGWCCG 1 cut(s) 275
CseI GACGC 1 cut(s) 163
CspI CGGWCCG 1 cut(s) 275
CviAII CATG 6 cut(s) 94, 236, 266, 349, 391, 527
DdeI CTNAG 1 cut(s) 302
DpnI GATC 4 cut(s) 45, 182, 199, 234
DpnII GATC 4 cut(s) 43, 180, 197, 232
DrdI GACNNNNNNGTC 1 cut(s) 10
DseDI GACNNNNNNGTC 1 cut(s) 10
Eam1104I CTCTTC 1 cut(s) 282
EarI CTCTTC 1 cut(s) 282
EciI GGCGGA 1 cut(s) 91
Eco31I GGTCTC 1 cut(s) 103
Eco47I GGWCC 1 cut(s) 275
EcoRII CCWGG 1 cut(s) 327
FaeI CATG 6 cut(s) 97, 239, 269, 352, 394, 530
FaiI YATR 8 cut(s) 95, 237, 267, 312, 350, 392, 513, 528
FatI CATG 6 cut(s) 93, 235, 265, 348, 390, 526
FauI CCCGC 2 cut(s) 416, 441
Fnu4HI GCNGC 4 cut(s) 28, 55, 358, 416
FokI GGATG 2 cut(s) 113, 131
Fsp4HI GCNGC 4 cut(s) 28, 55, 358, 416
FspBI CTAG 1 cut(s) 230
GluI GCNGC 4 cut(s) 28, 55, 358, 416
GsaI CCCAGC 1 cut(s) 27
HaeIII GGCC 1 cut(s) 332
HapII CCGG 4 cut(s) 207, 278, 381, 440
HgaI GACGC 1 cut(s) 163
Hin1I GRCGYC 1 cut(s) 174
Hin1II CATG 6 cut(s) 97, 239, 269, 352, 394, 530
HincII GTYRAC 1 cut(s) 247
HindII GTYRAC 1 cut(s) 247
HinfI GANTC 2 cut(s) 11, 117
HpaI GTTAAC 1 cut(s) 247
HpaII CCGG 4 cut(s) 207, 278, 381, 440
HphI GGTGA 3 cut(s) 100, 178, 236
Hpy166II GTNNAC 3 cut(s) 247, 316, 337
Hpy188I TCNGA 1 cut(s) 89
Hpy188III TCNNGA 4 cut(s) 178, 236, 349, 527
Hpy8I GTNNAC 3 cut(s) 247, 316, 337
HpyAV CCTTC 6 cut(s) 451, 463, 469, 475, 481, 487
HpyCH4IV ACGT 1 cut(s) 243
HpyCH4V TGCA 2 cut(s) 194, 257
HpyF10VI GCNNNNNNNGC 3 cut(s) 424, 445, 513
HpyF3I CTNAG 1 cut(s) 302
HpySE526I ACGT 1 cut(s) 243
Hsp92I GRCGYC 1 cut(s) 174
Hsp92II CATG 6 cut(s) 97, 239, 269, 352, 394, 530
KspAI GTTAAC 1 cut(s) 247
Kzo9I GATC 4 cut(s) 43, 180, 197, 232
LmnI GCTCC 3 cut(s) 146, 209, 412
Lsp1109I GCAGC 2 cut(s) 39, 66
LweI GCATC 1 cut(s) 532
MaeI CTAG 1 cut(s) 230
MaeII ACGT 1 cut(s) 243
MaeIII GTNAC 1 cut(s) 118
MalI GATC 4 cut(s) 45, 182, 199, 234
MboI GATC 4 cut(s) 43, 180, 197, 232
MboII GAAGA 4 cut(s) 179, 299, 475, 478
MflI RGATCY 1 cut(s) 197
MhlI GDGCHC 1 cut(s) 308
MlyI GAGTC 2 cut(s) 20, 126
MmeI TCCRAC 1 cut(s) 225
MnlI CCTC 8 cut(s) 44, 46, 148, 154, 213, 283, 356, 492
MseI TTAA 2 cut(s) 18, 246
MslI CAYNNNNRTG 1 cut(s) 311
MspI CCGG 4 cut(s) 207, 278, 381, 440
MspR9I CCNGG 2 cut(s) 329, 381
MvaI CCWGG 1 cut(s) 329
MwoI GCNNNNNNNGC 3 cut(s) 424, 445, 513
NciI CCSGG 1 cut(s) 381
NdeII GATC 4 cut(s) 43, 180, 197, 232
NlaIII CATG 6 cut(s) 97, 239, 269, 352, 394, 530
NlaIV GGNNCC 3 cut(s) 142, 199, 414
NmuCI GTSAC 1 cut(s) 118
OliI CACNNNNGTG 1 cut(s) 311
PagI TCATGA 3 cut(s) 235, 348, 526
PcsI WCGNNNNNNNCGW 1 cut(s) 171
PflFI GACNNNGTC 1 cut(s) 95
PkrI GCNGC 4 cut(s) 29, 56, 359, 417
PleI GAGTC 2 cut(s) 19, 125
PpsI GAGTC 2 cut(s) 19, 125
Psp1406I AACGTT 1 cut(s) 243
Psp6I CCWGG 1 cut(s) 327
PspFI CCCAGC 1 cut(s) 23
PspGI CCWGG 1 cut(s) 327
PspN4I GGNNCC 3 cut(s) 142, 199, 414
PspPI GGNCC 1 cut(s) 275
PsuI RGATCY 1 cut(s) 197
PsyI GACNNNGTC 1 cut(s) 95
RseI CAYNNNNRTG 1 cut(s) 311
Rsr2I CGGWCCG 1 cut(s) 275
RsrII CGGWCCG 1 cut(s) 275
SaqAI TTAA 2 cut(s) 18, 246
SatI GCNGC 4 cut(s) 28, 55, 358, 416
Sau3AI GATC 4 cut(s) 43, 180, 197, 232
Sau96I GGNCC 1 cut(s) 275
SchI GAGTC 2 cut(s) 20, 126
ScrFI CCNGG 2 cut(s) 329, 381
SduI GDGCHC 1 cut(s) 308
SetI ASST 4 cut(s) 214, 231, 246, 509
SfaNI GCATC 1 cut(s) 532
SinI GGWCC 1 cut(s) 275
SmiMI CAYNNNNRTG 1 cut(s) 311
SsiI CCGC 6 cut(s) 102, 273, 357, 416, 423, 448
SspMI CTAG 1 cut(s) 230
StyD4I CCNGG 2 cut(s) 327, 379
TaiI ACGT 1 cut(s) 246
TauI GCSGC 2 cut(s) 360, 418
Tru1I TTAA 2 cut(s) 18, 246
Tru9I TTAA 2 cut(s) 18, 246
TseFI GTSAC 1 cut(s) 118
TseI GCWGC 2 cut(s) 27, 54
Tsp45I GTSAC 1 cut(s) 118
TspDTI ATGAA 1 cut(s) 254
TspGWI ACGGA 2 cut(s) 63, 137
Tth111I GACNNNGTC 1 cut(s) 95
VpaK11BI GGWCC 1 cut(s) 275
XspI CTAG 1 cut(s) 230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.