AT1G21830

Protein of unknown function (DUF740)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0014253
Physical Location & Seq
Reverse (-)
7660947 .. 7662725
1779 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G21830.1

Sequence Viewer

Length: 621 bp
ATGGATATGTACAAAGATGATAGCTCACCATACTGTTATTTCCATCCAAAAGAAGAGTATGTTGGAGTTTGTCCTCTCTGTTTGAACGAGAGGCTTCTTGTTTTAGCTTCAAAACAGAGATCATCAAGAACCAAACACTCTTCCTCTTCACCAATCATCAGCCTCCCTAAGATATTCGCCTTGAGCTCTCTCCTCAGTCGCCTCGATCTCCGTCACCGGAAATTCCATCCCTCCTCCGATCTTGACGTCTCCACAAGCCAAGAAGATTCCTTTATATCGATAAAATTCGAAAACGACGGAAACGCGTCGTGGGAAAAGAAAGCAGTAGTGACAAAGGTTTGTGTAGACAACAACAACAACAACACAACAAACTCAACATGCAAGAAGCAGCAATCTCCTATAACGAGCAATACCAGTATCGTAGAGCACAATAGTGCCAAGTCATCGCTTAGATGGCGCAAGCGTATTGGTCATCTATTCCACGTCATCAAACTCAAATCAGGATCGTCCACGTCTTCTTGCCATGTGGCACCATCTAAGGTCGAAGGAACCAAAGTGAGGAAACAGGGTTGGATGGTGAGGACCTTAACCAGAAAAAAATCCAGGAAAAGTAAAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF740

Protein Analysis

206

Amino Acids

23.28

Weight (kDa)

9.94

Isoelectric Point (pI)

54.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF740 PF05340 8 - 60 5.3e-07 Protein of unknown function (DUF740)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014253)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21830
fragaria_vesca FvH4_2g26860
malus_domestica MD08G1163000.v1.1 MD15G1349700.v1.1
prunus_persica Prupe.1G498100_v2.0.a1
pyrus_communis pycom08g14080 pycom15g31280
rosa_chinensis RchiOBHm_Chr6g0295711
rosa_laevigata RLG00000011773
rosa_multiflora Rmu_sc0007372.1_g000002
rosa_roxburghii Rroxscaffold_7G00172190
rosa_rugosa Rorug06G0253700
rosa_samantha Rh6AG367100 Rh6BG374900 Rh6CG381200 Rh6DG368000
rosa_wichuraiana Rw6G032040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 249
AccB1I GGYRCC 1 cut(s) 529
AccI GTMKAC 1 cut(s) 345
AccII CGCG 1 cut(s) 305
AclWI GGATC 1 cut(s) 511
AcsI RAATTY 2 cut(s) 221, 284
AcyI GRCGYC 1 cut(s) 246
AfaI GTAC 1 cut(s) 11
AfiI CCNNNNNNNGG 1 cut(s) 558
AflIII ACRYGT 1 cut(s) 303
AgsI TTSAA 2 cut(s) 85, 111
AjiI CACGTC 2 cut(s) 484, 513
AjnI CCWGG 1 cut(s) 602
AjuI GAANNNNNNNTTGG 2 cut(s) 45, 77
AleI CACNNNNGTG 1 cut(s) 432
AluBI AGCT 3 cut(s) 24, 107, 186
AluI AGCT 3 cut(s) 24, 107, 186
Alw21I GWGCWC 2 cut(s) 188, 429
Alw26I GTCTC 1 cut(s) 253
AlwI GGATC 1 cut(s) 511
ApeKI GCWGC 1 cut(s) 388
ApoI RAATTY 2 cut(s) 221, 284
AspLEI GCGC 1 cut(s) 459
AspS9I GGNCC 1 cut(s) 582
AsuHPI GGTGA 4 cut(s) 18, 141, 206, 589
AsuII TTCGAA 1 cut(s) 288
AvaII GGWCC 1 cut(s) 582
BanI GGYRCC 1 cut(s) 529
BanII GRGCYC 1 cut(s) 188
BbsI GAAGAC 1 cut(s) 507
Bbv12I GWGCWC 2 cut(s) 188, 429
BbvI GCAGC 1 cut(s) 400
BccI CCATC 5 cut(s) 51, 234, 447, 541, 568
BciT130I CCWGG 1 cut(s) 604
BcoDI GTCTC 1 cut(s) 253
BisI GCNGC 1 cut(s) 389
BlsI GCNGC 1 cut(s) 390
Bme1390I CCNGG 1 cut(s) 604
Bme18I GGWCC 1 cut(s) 582
BmgBI CACGTC 2 cut(s) 484, 513
BmgT120I GGNCC 1 cut(s) 582
BmiI GGNNCC 2 cut(s) 531, 550
BmrFI CCNGG 1 cut(s) 604
BpiI GAAGAC 1 cut(s) 507
Bpu14I TTCGAA 1 cut(s) 288
BpuEI CTTGAG 1 cut(s) 202
Bsa29I ATCGAT 1 cut(s) 278
BsaHI GRCGYC 1 cut(s) 246
BsaWI WCCGGW 1 cut(s) 216
Bsc4I CCNNNNNNNGG 1 cut(s) 558
Bse1I ACTGG 1 cut(s) 414
BseBI CCWGG 1 cut(s) 604
BseCI ATCGAT 1 cut(s) 278
BseGI GGATG 3 cut(s) 43, 226, 579
BseLI CCNNNNNNNGG 1 cut(s) 558
BseMII CTCAG 1 cut(s) 208
BseNI ACTGG 1 cut(s) 414
BseRI GAGGAG 2 cut(s) 182, 223
BseXI GCAGC 1 cut(s) 400
Bsh1236I CGCG 1 cut(s) 305
BshNI GGYRCC 1 cut(s) 529
BshVI ATCGAT 1 cut(s) 278
BsiHKAI GWGCWC 2 cut(s) 188, 429
BsiSI CCGG 1 cut(s) 217
BslI CCNNNNNNNGG 1 cut(s) 558
BsmAI GTCTC 1 cut(s) 253
BsmBI CGTCTC 1 cut(s) 253
Bsp119I TTCGAA 1 cut(s) 288
Bsp1286I GDGCHC 2 cut(s) 188, 429
Bsp1407I TGTACA 1 cut(s) 9
Bsp143I GATC 4 cut(s) 119, 205, 238, 503
BspCNI CTCAG 1 cut(s) 207
BspDI ATCGAT 1 cut(s) 278
BspFNI CGCG 1 cut(s) 305
BspLI GGNNCC 2 cut(s) 531, 550
BspPI GGATC 1 cut(s) 511
BspT104I TTCGAA 1 cut(s) 288
BspT107I GGYRCC 1 cut(s) 529
BsrGI TGTACA 1 cut(s) 9
BsrI ACTGG 1 cut(s) 414
BssMI GATC 4 cut(s) 119, 205, 238, 503
BssNI GRCGYC 1 cut(s) 246
Bst2UI CCWGG 1 cut(s) 604
Bst4CI ACNGT 1 cut(s) 35
Bst6I CTCTTC 3 cut(s) 48, 145, 151
BstACI GRCGYC 1 cut(s) 246
BstAUI TGTACA 1 cut(s) 9
BstBI TTCGAA 1 cut(s) 288
BstC8I GCNNGC 1 cut(s) 461
BstDEI CTNAG 4 cut(s) 168, 194, 449, 537
BstF5I GGATG 3 cut(s) 43, 226, 579
BstFNI CGCG 1 cut(s) 305
BstHHI GCGC 1 cut(s) 459
BstKTI GATC 4 cut(s) 122, 208, 241, 506
BstMAI GTCTC 1 cut(s) 253
BstMBI GATC 4 cut(s) 119, 205, 238, 503
BstMWI GCNNNNNNNGC 1 cut(s) 454
BstNI CCWGG 1 cut(s) 604
BstNSI RCATGY 1 cut(s) 381
BstSCI CCNGG 1 cut(s) 602
BstUI CGCG 1 cut(s) 305
BstV1I GCAGC 1 cut(s) 400
BstV2I GAAGAC 1 cut(s) 507
Bsu15I ATCGAT 1 cut(s) 278
BsuTUI ATCGAT 1 cut(s) 278
BtgZI GCGATG 1 cut(s) 429
BtrI CACGTC 2 cut(s) 484, 513
BtsCI GGATG 3 cut(s) 43, 226, 579
Cac8I GCNNGC 1 cut(s) 461
CfoI GCGC 1 cut(s) 459
Cfr13I GGNCC 1 cut(s) 582
ClaI ATCGAT 1 cut(s) 278
CseI GACGC 1 cut(s) 294
Csp6I GTAC 1 cut(s) 10
CviAII CATG 2 cut(s) 378, 524
CviJI RGCY 6 cut(s) 24, 94, 107, 162, 186, 258
CviKI_1 RGCY 6 cut(s) 24, 94, 107, 162, 186, 258
CviQI GTAC 1 cut(s) 10
DdeI CTNAG 4 cut(s) 168, 194, 449, 537
DpnI GATC 4 cut(s) 121, 207, 240, 505
DpnII GATC 4 cut(s) 119, 205, 238, 503
Eam1104I CTCTTC 3 cut(s) 48, 145, 151
EarI CTCTTC 3 cut(s) 48, 145, 151
Ecl136II GAGCTC 1 cut(s) 186
Eco24I GRGCYC 1 cut(s) 188
Eco47I GGWCC 1 cut(s) 582
Eco53kI GAGCTC 1 cut(s) 186
EcoICRI GAGCTC 1 cut(s) 186
EcoO109I RGGNCCY 1 cut(s) 582
EcoRII CCWGG 1 cut(s) 602
EcoT38I GRGCYC 1 cut(s) 188
Esp3I CGTCTC 1 cut(s) 253
FaeI CATG 2 cut(s) 381, 527
FaiI YATR 7 cut(s) 8, 31, 60, 275, 379, 401, 525
FatI CATG 2 cut(s) 377, 523
FblI GTMKAC 1 cut(s) 345
Fnu4HI GCNGC 1 cut(s) 389
FokI GGATG 3 cut(s) 30, 213, 586
FriOI GRGCYC 1 cut(s) 188
Fsp4HI GCNGC 1 cut(s) 389
GlaI GCGC 1 cut(s) 458
GluI GCNGC 1 cut(s) 389
HapII CCGG 1 cut(s) 217
HgaI GACGC 1 cut(s) 294
HhaI GCGC 1 cut(s) 459
Hin1I GRCGYC 1 cut(s) 246
Hin1II CATG 2 cut(s) 381, 527
Hin6I GCGC 1 cut(s) 457
HinP1I GCGC 1 cut(s) 457
HinfI GANTC 1 cut(s) 266
HpaII CCGG 1 cut(s) 217
HphI GGTGA 4 cut(s) 18, 141, 206, 589
Hpy166II GTNNAC 2 cut(s) 346, 510
Hpy188I TCNGA 1 cut(s) 238
Hpy188III TCNNGA 3 cut(s) 126, 242, 501
Hpy8I GTNNAC 2 cut(s) 346, 510
Hpy99I CGWCG 2 cut(s) 299, 310
HpyAV CCTTC 1 cut(s) 539
HpyCH4III ACNGT 1 cut(s) 35
HpyCH4IV ACGT 3 cut(s) 246, 483, 512
HpyCH4V TGCA 1 cut(s) 381
HpyF10VI GCNNNNNNNGC 1 cut(s) 454
HpyF3I CTNAG 4 cut(s) 168, 194, 449, 537
HpySE526I ACGT 3 cut(s) 246, 483, 512
Hsp92I GRCGYC 1 cut(s) 246
Hsp92II CATG 2 cut(s) 381, 527
HspAI GCGC 1 cut(s) 457
Kzo9I GATC 4 cut(s) 119, 205, 238, 503
LpnPI CCDG 7 cut(s) 230, 427, 486, 551, 589, 604, 616
Lsp1109I GCAGC 1 cut(s) 400
MaeII ACGT 3 cut(s) 246, 483, 512
MaeIII GTNAC 2 cut(s) 212, 328
MalI GATC 4 cut(s) 121, 207, 240, 505
MboI GATC 4 cut(s) 119, 205, 238, 503
MboII GAAGA 5 cut(s) 65, 132, 138, 275, 507
MhlI GDGCHC 2 cut(s) 188, 429
MluCI AATT 2 cut(s) 221, 284
MluI ACGCGT 1 cut(s) 303
MmeI TCCRAC 2 cut(s) 43, 551
MseI TTAA 1 cut(s) 587
MslI CAYNNNNRTG 1 cut(s) 432
MspI CCGG 1 cut(s) 217
MspR9I CCNGG 1 cut(s) 604
MvaI CCWGG 1 cut(s) 604
MvnI CGCG 1 cut(s) 305
MwoI GCNNNNNNNGC 1 cut(s) 454
NdeII GATC 4 cut(s) 119, 205, 238, 503
NlaIII CATG 2 cut(s) 381, 527
NlaIV GGNNCC 2 cut(s) 531, 550
NmuCI GTSAC 2 cut(s) 212, 328
NspI RCATGY 1 cut(s) 381
NspV TTCGAA 1 cut(s) 288
OliI CACNNNNGTG 1 cut(s) 432
PfeI GAWTC 1 cut(s) 266
PfoI TCCNGGA 1 cut(s) 602
PkrI GCNGC 1 cut(s) 390
PpuMI RGGWCCY 1 cut(s) 582
Psp124BI GAGCTC 1 cut(s) 188
Psp5II RGGWCCY 1 cut(s) 582
Psp6I CCWGG 1 cut(s) 602
PspGI CCWGG 1 cut(s) 602
PspN4I GGNNCC 2 cut(s) 531, 550
PspPI GGNCC 1 cut(s) 582
PspPPI RGGWCCY 1 cut(s) 582
RsaI GTAC 1 cut(s) 11
RsaNI GTAC 1 cut(s) 10
RseI CAYNNNNRTG 1 cut(s) 432
SacI GAGCTC 1 cut(s) 188
SaqAI TTAA 1 cut(s) 587
SatI GCNGC 1 cut(s) 389
Sau3AI GATC 4 cut(s) 119, 205, 238, 503
Sau96I GGNCC 1 cut(s) 582
ScrFI CCNGG 1 cut(s) 604
SduI GDGCHC 2 cut(s) 188, 429
SetI ASST 9 cut(s) 26, 109, 188, 249, 339, 486, 515, 543, 587
SfuI TTCGAA 1 cut(s) 288
SinI GGWCC 1 cut(s) 582
SmiMI CAYNNNNRTG 1 cut(s) 432
SmlI CTYRAG 1 cut(s) 181
SmoI CTYRAG 1 cut(s) 181
Sse9I AATT 2 cut(s) 221, 284
SstI GAGCTC 1 cut(s) 188
StyD4I CCNGG 1 cut(s) 602
TaaI ACNGT 1 cut(s) 35
TaiI ACGT 3 cut(s) 249, 486, 515
TaqI TCGA 4 cut(s) 204, 278, 288, 543
TasI AATT 2 cut(s) 221, 284
TatI WGTACW 1 cut(s) 9
TfiI GAWTC 1 cut(s) 266
Tru1I TTAA 1 cut(s) 587
Tru9I TTAA 1 cut(s) 587
TseFI GTSAC 2 cut(s) 212, 328
TseI GCWGC 1 cut(s) 388
Tsp45I GTSAC 2 cut(s) 212, 328
TspGWI ACGGA 2 cut(s) 200, 312
VpaK11BI GGWCC 1 cut(s) 582
XapI RAATTY 2 cut(s) 221, 284
XceI RCATGY 1 cut(s) 381
XmiI GTMKAC 1 cut(s) 345
ZraI GACGTC 1 cut(s) 247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.