MD08G1163000.v1.1

Protein of unknown function (DUF740)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0014253
Physical Location & Seq
Reverse (-)
19191020 .. 19193820
2801 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1163000.v1.1.491

Sequence Viewer

Length: 642 bp
ATGAATGGGTACAAAGAAGACACCTCCTTTTGCTATTTCCACCCCAAAGAGCTGGTTGTGGGGGTCTGCTCCCTCTGCCTGACTGAGAGGCTTCTAATCTTGGCTTCCAAAAAAGGCCACCACCACCACCGCCACCATGTCTCATCCGTCGGAAGCGGCAGTGCCCATAAAAACCCAAATGGTATGCACAAGAAGGCACCCATTAGCCTCCCCAAGATCTTTGCTTTCAGCTCTCTCCTCAATCGGCAGGGGAAACCAGACGACGGCCCAGATCAAGAGGCTTCCACCAGCCAAGAAGACTCATTCATATCAATCAAGTTTGAAGACAATGGAGTGGCCTCATGGGAAAAGAACAAAGTGTCTAAGGTCTCTCTAGATCATTGCAACATGTCCTGGAATCACAACCTGAGCAAGGAAGCCAAGGAAACAAAGGAGGCAAAGGATAGCAAGAGCGTGATAGAGCACGGGAAATCCCACATCCCGCTCAGGTGGCGGAAGAGGATTGGTCACTTGGCCCAGCTCATCAGATGGAAGAGGTCGAACAAGGGCAGTGTGTGTCATGTCGGCAACAAGGCGGAGGGAGTCAAGGTAAGGAAGGGTTGGATAAGAAACCTGACAAAGAGGAGAGGGGCCATGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF740

Protein Analysis

214

Amino Acids

24.03

Weight (kDa)

9.85

Isoelectric Point (pI)

47.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014253)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21830
fragaria_vesca FvH4_2g26860
malus_domestica MD08G1163000.v1.1 MD15G1349700.v1.1
prunus_persica Prupe.1G498100_v2.0.a1
pyrus_communis pycom08g14080 pycom15g31280
rosa_chinensis RchiOBHm_Chr6g0295711
rosa_laevigata RLG00000011773
rosa_multiflora Rmu_sc0007372.1_g000002
rosa_roxburghii Rroxscaffold_7G00172190
rosa_rugosa Rorug06G0253700
rosa_samantha Rh6AG367100 Rh6BG374900 Rh6CG381200 Rh6DG368000
rosa_wichuraiana Rw6G032040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 196
AccBSI CCGCTC 1 cut(s) 484
AciI CCGC 5 cut(s) 130, 156, 482, 493, 575
AfaI GTAC 1 cut(s) 11
AfiI CCNNNNNNNGG 2 cut(s) 263, 412
AflIII ACRYGT 1 cut(s) 387
AgsI TTSAA 1 cut(s) 323
AjnI CCWGG 1 cut(s) 392
AluBI AGCT 3 cut(s) 52, 231, 520
AluI AGCT 3 cut(s) 52, 231, 520
Alw21I GWGCWC 1 cut(s) 465
Alw26I GTCTC 2 cut(s) 145, 373
AoxI GGCC 5 cut(s) 115, 265, 336, 513, 630
AspS9I GGNCC 3 cut(s) 266, 514, 630
BaeGI GKGCMC 1 cut(s) 166
BanI GGYRCC 1 cut(s) 196
BbsI GAAGAC 3 cut(s) 24, 303, 330
Bbv12I GWGCWC 1 cut(s) 465
BccI CCATC 1 cut(s) 522
BceAI ACGGC 1 cut(s) 280
BciT130I CCWGG 1 cut(s) 394
BcoDI GTCTC 2 cut(s) 145, 373
BfaI CTAG 1 cut(s) 374
BglII AGATCT 1 cut(s) 216
BisI GCNGC 1 cut(s) 157
BlsI GCNGC 1 cut(s) 158
Bme1390I CCNGG 1 cut(s) 394
BmgT120I GGNCC 3 cut(s) 266, 514, 630
BmiI GGNNCC 2 cut(s) 198, 631
BmrFI CCNGG 1 cut(s) 394
BpiI GAAGAC 3 cut(s) 24, 303, 330
Bpu10I CCTNAGC 2 cut(s) 407, 485
BsaI GGTCTC 1 cut(s) 373
BsaJI CCNNGG 2 cut(s) 420, 633
BsaXI ACNNNNNCTCC 2 cut(s) 573, 603
Bsc4I CCNNNNNNNGG 2 cut(s) 263, 412
Bse3DI GCAATG 1 cut(s) 379
BseBI CCWGG 1 cut(s) 394
BseDI CCNNGG 2 cut(s) 420, 633
BseGI GGATG 2 cut(s) 143, 477
BseLI CCNNNNNNNGG 2 cut(s) 263, 412
BseMI GCAATG 1 cut(s) 379
BseMII CTCAG 3 cut(s) 75, 398, 499
BseRI GAGGAG 2 cut(s) 227, 637
BseSI GKGCMC 1 cut(s) 166
BseYI CCCAGC 1 cut(s) 516
BshFI GGCC 5 cut(s) 117, 267, 338, 515, 632
BshNI GGYRCC 1 cut(s) 196
BsiHKAI GWGCWC 1 cut(s) 465
BslI CCNNNNNNNGG 2 cut(s) 263, 412
BsmAI GTCTC 2 cut(s) 145, 373
BsnI GGCC 5 cut(s) 117, 267, 338, 515, 632
Bso31I GGTCTC 1 cut(s) 373
Bsp1286I GDGCHC 2 cut(s) 166, 465
Bsp143I GATC 3 cut(s) 216, 271, 376
Bsp19I CCATGG 1 cut(s) 633
BspACI CCGC 5 cut(s) 130, 156, 482, 493, 575
BspANI GGCC 5 cut(s) 117, 267, 338, 515, 632
BspCNI CTCAG 3 cut(s) 76, 399, 498
BspLI GGNNCC 2 cut(s) 198, 631
BspT107I GGYRCC 1 cut(s) 196
BspTNI GGTCTC 1 cut(s) 373
BsrBI CCGCTC 1 cut(s) 484
BsrDI GCAATG 1 cut(s) 379
BssECI CCNNGG 2 cut(s) 420, 633
BssMI GATC 3 cut(s) 216, 271, 376
BssT1I CCWWGG 2 cut(s) 420, 633
Bst2UI CCWGG 1 cut(s) 394
Bst6I CTCTTC 2 cut(s) 491, 527
BstDEI CTNAG 4 cut(s) 84, 363, 407, 485
BstDSI CCRYGG 1 cut(s) 633
BstENI CCTNNNNNAGG 1 cut(s) 410
BstF5I GGATG 2 cut(s) 143, 477
BstKTI GATC 3 cut(s) 219, 274, 379
BstMAI GTCTC 2 cut(s) 145, 373
BstMBI GATC 3 cut(s) 216, 271, 376
BstMWI GCNNNNNNNGC 2 cut(s) 75, 490
BstNI CCWGG 1 cut(s) 394
BstNSI RCATGY 1 cut(s) 391
BstSCI CCNGG 1 cut(s) 392
BstSLI GKGCMC 1 cut(s) 166
BstV2I GAAGAC 3 cut(s) 24, 303, 330
BstX2I RGATCY 1 cut(s) 216
BstXI CCANNNNNNTGG 1 cut(s) 52
BstYI RGATCY 1 cut(s) 216
BsuRI GGCC 5 cut(s) 117, 267, 338, 515, 632
BtgI CCRYGG 1 cut(s) 633
BtsCI GGATG 2 cut(s) 143, 477
BtsI GCAGTG 2 cut(s) 166, 556
BtsIMutI CAGTG 2 cut(s) 166, 556
Cfr13I GGNCC 3 cut(s) 266, 514, 630
Csp6I GTAC 1 cut(s) 10
CviAII CATG 5 cut(s) 137, 342, 388, 560, 634
CviQI GTAC 1 cut(s) 10
DdeI CTNAG 4 cut(s) 84, 363, 407, 485
DpnI GATC 3 cut(s) 218, 273, 378
DpnII GATC 3 cut(s) 216, 271, 376
Eam1104I CTCTTC 2 cut(s) 491, 527
EarI CTCTTC 2 cut(s) 491, 527
EciI GGCGGA 2 cut(s) 508, 590
Eco130I CCWWGG 2 cut(s) 420, 633
Eco31I GGTCTC 1 cut(s) 373
EcoNI CCTNNNNNAGG 1 cut(s) 410
EcoRII CCWGG 1 cut(s) 392
EcoT14I CCWWGG 2 cut(s) 420, 633
ErhI CCWWGG 2 cut(s) 420, 633
FaeI CATG 5 cut(s) 140, 345, 391, 563, 637
FaiI YATR 8 cut(s) 138, 168, 185, 308, 343, 389, 561, 635
FatI CATG 5 cut(s) 136, 341, 387, 559, 633
FauI CCCGC 1 cut(s) 489
Fnu4HI GCNGC 1 cut(s) 157
FokI GGATG 2 cut(s) 130, 464
Fsp4HI GCNGC 1 cut(s) 157
FspBI CTAG 1 cut(s) 374
GluI GCNGC 1 cut(s) 157
GsaI CCCAGC 1 cut(s) 520
HaeIII GGCC 5 cut(s) 117, 267, 338, 515, 632
Hin1II CATG 5 cut(s) 140, 345, 391, 563, 637
HinfI GANTC 3 cut(s) 299, 397, 582
Hpy188I TCNGA 2 cut(s) 152, 527
Hpy188III TCNNGA 2 cut(s) 275, 374
Hpy99I CGWCG 2 cut(s) 152, 266
HpyAV CCTTC 2 cut(s) 187, 589
HpyCH4V TGCA 2 cut(s) 187, 384
HpyF10VI GCNNNNNNNGC 2 cut(s) 75, 490
HpyF3I CTNAG 4 cut(s) 84, 363, 407, 485
Hsp92II CATG 5 cut(s) 140, 345, 391, 563, 637
Kzo9I GATC 3 cut(s) 216, 271, 376
LmnI GCTCC 1 cut(s) 74
MaeI CTAG 1 cut(s) 374
MaeIII GTNAC 1 cut(s) 506
MalI GATC 3 cut(s) 218, 273, 378
MbiI CCGCTC 1 cut(s) 484
MboI GATC 3 cut(s) 216, 271, 376
MboII GAAGA 5 cut(s) 29, 308, 335, 508, 544
MflI RGATCY 1 cut(s) 216
MhlI GDGCHC 2 cut(s) 166, 465
MlyI GAGTC 2 cut(s) 293, 591
MmeI TCCRAC 2 cut(s) 130, 581
MspR9I CCNGG 1 cut(s) 394
MvaI CCWGG 1 cut(s) 394
MwoI GCNNNNNNNGC 2 cut(s) 75, 490
NcoI CCATGG 1 cut(s) 633
NdeII GATC 3 cut(s) 216, 271, 376
NlaIII CATG 5 cut(s) 140, 345, 391, 563, 637
NlaIV GGNNCC 2 cut(s) 198, 631
NmuCI GTSAC 1 cut(s) 506
NspI RCATGY 1 cut(s) 391
PciI ACATGT 1 cut(s) 387
PfeI GAWTC 1 cut(s) 397
PfoI TCCNGGA 1 cut(s) 392
PkrI GCNGC 1 cut(s) 158
PleI GAGTC 2 cut(s) 293, 590
PpsI GAGTC 2 cut(s) 293, 590
PscI ACATGT 1 cut(s) 387
Psp6I CCWGG 1 cut(s) 392
PspFI CCCAGC 1 cut(s) 516
PspGI CCWGG 1 cut(s) 392
PspN4I GGNNCC 2 cut(s) 198, 631
PspPI GGNCC 3 cut(s) 266, 514, 630
PsuI RGATCY 1 cut(s) 216
RsaI GTAC 1 cut(s) 11
RsaNI GTAC 1 cut(s) 10
SatI GCNGC 1 cut(s) 157
Sau3AI GATC 3 cut(s) 216, 271, 376
Sau96I GGNCC 3 cut(s) 266, 514, 630
SchI GAGTC 2 cut(s) 293, 591
ScrFI CCNGG 1 cut(s) 394
SduI GDGCHC 2 cut(s) 166, 465
SsiI CCGC 5 cut(s) 130, 156, 482, 493, 575
SspMI CTAG 1 cut(s) 374
StyD4I CCNGG 1 cut(s) 392
StyI CCWWGG 2 cut(s) 420, 633
TaqI TCGA 1 cut(s) 539
TauI GCSGC 1 cut(s) 159
TfiI GAWTC 1 cut(s) 397
TscAI CASTG 2 cut(s) 166, 556
TseFI GTSAC 1 cut(s) 506
Tsp45I GTSAC 1 cut(s) 506
TspDTI ATGAA 2 cut(s) 17, 295
TspGWI ACGGA 1 cut(s) 136
TspRI CASTG 2 cut(s) 166, 556
XagI CCTNNNNNAGG 1 cut(s) 410
XbaI TCTAGA 1 cut(s) 373
XceI RCATGY 1 cut(s) 391
XspI CTAG 1 cut(s) 374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.