MD15G1349700.v1.1

Protein of unknown function (DUF740)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0014253
Physical Location & Seq
Reverse (-)
41472905 .. 41475468
2564 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1349700.v1.1.491

Sequence Viewer

Length: 651 bp
ATGAATGGCTTCAGAGAAGAGAACTCCTTTTGCTATTTCCACCCTAAAGAGTTGGTTGTGGGGGTCTGCTCGCTCTGCCTGACTGAGAGGCTTCTGATCTTGTCAGCCAAAAAAGGCCGCCACCACCACCGCCACCATGTCTCCTCCATCAGAGGTGGCAGAGGCGGCAGTGGCCATAAAAACCCAAATGGCATGCACAAGAAGGCACCCATTAGCCTCCCCAAGATCTTTGCTTTCAGCTCTTTCCTCAATCGGCAGTGGAAACCTGACGACGGCTCAGATCAAGAGGCTTCCACTAGCCAAGAAGACTCATTCATATCAATCAAGTTTGAAGACAATGGAGTAGCCTCATGGGAGAAGAACAAAGTCTCTAAGGTCTCTCTAGATCATTGCAACATGTCCTGGAATCACAACTTCAGCAAGGAAGCCAAGGAAACCAAGGAGGCAAGGGATACCAAGAGCGTGATAGAGTACGGGAAATCCCACATCCCGCTTAGGTGGCGGAAGAGGATTGGTCACTTGACCCAGCTCATCAGATGGAAGAGGTCCAACAAGGGCAGTGTGTGCCATGTCAGCAACAAGGTGGAGGGTGTCAAGGTAAGGAAGGGTTGGATAAGAAGCTTGACAAAGAGGAGATGGGCCATGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF740

Protein Analysis

217

Amino Acids

24.87

Weight (kDa)

10.08

Isoelectric Point (pI)

53.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014253)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21830
fragaria_vesca FvH4_2g26860
malus_domestica MD08G1163000.v1.1 MD15G1349700.v1.1
prunus_persica Prupe.1G498100_v2.0.a1
pyrus_communis pycom08g14080 pycom15g31280
rosa_chinensis RchiOBHm_Chr6g0295711
rosa_laevigata RLG00000011773
rosa_multiflora Rmu_sc0007372.1_g000002
rosa_roxburghii Rroxscaffold_7G00172190
rosa_rugosa Rorug06G0253700
rosa_samantha Rh6AG367100 Rh6BG374900 Rh6CG381200 Rh6DG368000
rosa_wichuraiana Rw6G032040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 205
AciI CCGC 5 cut(s) 118, 130, 165, 491, 502
AcoI YGGCCR 1 cut(s) 172
AcuI CTGAAG 1 cut(s) 400
AfaI GTAC 1 cut(s) 473
AfiI CCNNNNNNNGG 1 cut(s) 272
AflIII ACRYGT 1 cut(s) 396
AgsI TTSAA 1 cut(s) 332
AjnI CCWGG 1 cut(s) 401
AluBI AGCT 3 cut(s) 240, 529, 621
AluI AGCT 3 cut(s) 240, 529, 621
Alw26I GTCTC 3 cut(s) 145, 373, 382
AoxI GGCC 3 cut(s) 115, 172, 639
Asp700I GAANNNNTTC 1 cut(s) 8
AspS9I GGNCC 2 cut(s) 546, 639
AvaII GGWCC 1 cut(s) 546
BalI TGGCCA 1 cut(s) 174
BanI GGYRCC 1 cut(s) 205
BbsI GAAGAC 2 cut(s) 312, 339
BccI CCATC 3 cut(s) 155, 531, 630
BceAI ACGGC 1 cut(s) 289
BciT130I CCWGG 1 cut(s) 403
BciVI GTATCC 1 cut(s) 445
BcoDI GTCTC 3 cut(s) 145, 373, 382
BfaI CTAG 2 cut(s) 297, 383
BfuI GTATCC 1 cut(s) 445
BglII AGATCT 1 cut(s) 225
BisI GCNGC 2 cut(s) 118, 166
BlsI GCNGC 2 cut(s) 119, 167
Bme1390I CCNGG 1 cut(s) 403
Bme18I GGWCC 1 cut(s) 546
BmgT120I GGNCC 2 cut(s) 546, 639
BmiI GGNNCC 1 cut(s) 207
BmrFI CCNGG 1 cut(s) 403
BpiI GAAGAC 2 cut(s) 312, 339
Bpu10I CCTNAGC 1 cut(s) 494
BsaI GGTCTC 1 cut(s) 382
BsaJI CCNNGG 3 cut(s) 429, 438, 642
BsaXI ACNNNNNCTCC 2 cut(s) 125, 155
Bsc4I CCNNNNNNNGG 1 cut(s) 272
Bse3DI GCAATG 1 cut(s) 388
BseBI CCWGG 1 cut(s) 403
BseDI CCNNGG 3 cut(s) 429, 438, 642
BseGI GGATG 1 cut(s) 486
BseLI CCNNNNNNNGG 1 cut(s) 272
BseMI GCAATG 1 cut(s) 388
BseMII CTCAG 2 cut(s) 75, 291
BseRI GAGGAG 2 cut(s) 133, 646
BseYI CCCAGC 1 cut(s) 525
BshFI GGCC 3 cut(s) 117, 174, 641
BshNI GGYRCC 1 cut(s) 205
BslI CCNNNNNNNGG 1 cut(s) 272
BsmAI GTCTC 3 cut(s) 145, 373, 382
BsnI GGCC 3 cut(s) 117, 174, 641
Bso31I GGTCTC 1 cut(s) 382
Bsp143I GATC 4 cut(s) 96, 225, 280, 385
Bsp19I CCATGG 1 cut(s) 642
BspACI CCGC 5 cut(s) 118, 130, 165, 491, 502
BspANI GGCC 3 cut(s) 117, 174, 641
BspCNI CTCAG 2 cut(s) 76, 290
BspLI GGNNCC 1 cut(s) 207
BspT107I GGYRCC 1 cut(s) 205
BspTNI GGTCTC 1 cut(s) 382
BsrDI GCAATG 1 cut(s) 388
BssECI CCNNGG 3 cut(s) 429, 438, 642
BssMI GATC 4 cut(s) 96, 225, 280, 385
BssT1I CCWWGG 3 cut(s) 429, 438, 642
Bst2UI CCWGG 1 cut(s) 403
Bst6I CTCTTC 3 cut(s) 12, 500, 536
BstAPI GCANNNNNTGC 1 cut(s) 564
BstC8I GCNNGC 2 cut(s) 71, 194
BstDEI CTNAG 4 cut(s) 84, 277, 372, 494
BstDSI CCRYGG 1 cut(s) 642
BstF5I GGATG 1 cut(s) 486
BstKTI GATC 4 cut(s) 99, 228, 283, 388
BstMAI GTCTC 3 cut(s) 145, 373, 382
BstMBI GATC 4 cut(s) 96, 225, 280, 385
BstMWI GCNNNNNNNGC 6 cut(s) 75, 165, 171, 499, 564, 573
BstNI CCWGG 1 cut(s) 403
BstNSI RCATGY 2 cut(s) 196, 400
BstSCI CCNGG 1 cut(s) 401
BstV2I GAAGAC 2 cut(s) 312, 339
BstX2I RGATCY 1 cut(s) 225
BstYI RGATCY 1 cut(s) 225
BsuI GTATCC 1 cut(s) 445
BsuRI GGCC 3 cut(s) 117, 174, 641
BtgI CCRYGG 1 cut(s) 642
BtsCI GGATG 1 cut(s) 486
BtsI GCAGTG 3 cut(s) 175, 263, 565
BtsIMutI CAGTG 3 cut(s) 175, 263, 565
Cac8I GCNNGC 2 cut(s) 71, 194
Cfr13I GGNCC 2 cut(s) 546, 639
Csp6I GTAC 1 cut(s) 472
CviAII CATG 6 cut(s) 137, 193, 351, 397, 569, 643
CviQI GTAC 1 cut(s) 472
DdeI CTNAG 4 cut(s) 84, 277, 372, 494
DpnI GATC 4 cut(s) 98, 227, 282, 387
DpnII GATC 4 cut(s) 96, 225, 280, 385
EaeI YGGCCR 1 cut(s) 172
Eam1104I CTCTTC 3 cut(s) 12, 500, 536
EarI CTCTTC 3 cut(s) 12, 500, 536
EciI GGCGGA 1 cut(s) 517
Eco130I CCWWGG 3 cut(s) 429, 438, 642
Eco31I GGTCTC 1 cut(s) 382
Eco47I GGWCC 1 cut(s) 546
Eco57I CTGAAG 1 cut(s) 400
EcoRII CCWGG 1 cut(s) 401
EcoT14I CCWWGG 3 cut(s) 429, 438, 642
ErhI CCWWGG 3 cut(s) 429, 438, 642
FaeI CATG 6 cut(s) 140, 196, 354, 400, 572, 646
FaiI YATR 8 cut(s) 138, 177, 194, 317, 352, 398, 570, 644
FatI CATG 6 cut(s) 136, 192, 350, 396, 568, 642
FauI CCCGC 1 cut(s) 498
Fnu4HI GCNGC 2 cut(s) 118, 166
FokI GGATG 1 cut(s) 473
Fsp4HI GCNGC 2 cut(s) 118, 166
FspBI CTAG 2 cut(s) 297, 383
GluI GCNGC 2 cut(s) 118, 166
GsaI CCCAGC 1 cut(s) 529
HaeIII GGCC 3 cut(s) 117, 174, 641
Hin1II CATG 6 cut(s) 140, 196, 354, 400, 572, 646
HindIII AAGCTT 1 cut(s) 619
HinfI GANTC 2 cut(s) 308, 406
Hpy188I TCNGA 5 cut(s) 14, 96, 152, 280, 536
Hpy188III TCNNGA 2 cut(s) 284, 383
Hpy99I CGWCG 1 cut(s) 275
HpyAV CCTTC 2 cut(s) 196, 598
HpyCH4V TGCA 2 cut(s) 196, 393
HpyF10VI GCNNNNNNNGC 6 cut(s) 75, 165, 171, 499, 564, 573
HpyF3I CTNAG 4 cut(s) 84, 277, 372, 494
Hsp92II CATG 6 cut(s) 140, 196, 354, 400, 572, 646
Kzo9I GATC 4 cut(s) 96, 225, 280, 385
LpnPI CCDG 5 cut(s) 92, 279, 388, 415, 539
MaeI CTAG 2 cut(s) 297, 383
MaeIII GTNAC 1 cut(s) 515
MalI GATC 4 cut(s) 98, 227, 282, 387
MboI GATC 4 cut(s) 96, 225, 280, 385
MboII GAAGA 6 cut(s) 29, 317, 344, 370, 517, 553
MflI RGATCY 1 cut(s) 225
MlsI TGGCCA 1 cut(s) 174
MluNI TGGCCA 1 cut(s) 174
MlyI GAGTC 1 cut(s) 302
MmeI TCCRAC 2 cut(s) 573, 590
Mox20I TGGCCA 1 cut(s) 174
MroXI GAANNNNTTC 1 cut(s) 8
MscI TGGCCA 1 cut(s) 174
Msp20I TGGCCA 1 cut(s) 174
MspR9I CCNGG 1 cut(s) 403
MvaI CCWGG 1 cut(s) 403
MwoI GCNNNNNNNGC 6 cut(s) 75, 165, 171, 499, 564, 573
NcoI CCATGG 1 cut(s) 642
NdeII GATC 4 cut(s) 96, 225, 280, 385
NlaIII CATG 6 cut(s) 140, 196, 354, 400, 572, 646
NlaIV GGNNCC 1 cut(s) 207
NmuCI GTSAC 1 cut(s) 515
NspI RCATGY 2 cut(s) 196, 400
PaeI GCATGC 1 cut(s) 196
PciI ACATGT 1 cut(s) 396
PdmI GAANNNNTTC 1 cut(s) 8
PfeI GAWTC 1 cut(s) 406
PfoI TCCNGGA 1 cut(s) 401
PkrI GCNGC 2 cut(s) 119, 167
PleI GAGTC 1 cut(s) 302
PpsI GAGTC 1 cut(s) 302
PscI ACATGT 1 cut(s) 396
Psp6I CCWGG 1 cut(s) 401
PspFI CCCAGC 1 cut(s) 525
PspGI CCWGG 1 cut(s) 401
PspN4I GGNNCC 1 cut(s) 207
PspPI GGNCC 2 cut(s) 546, 639
PsuI RGATCY 1 cut(s) 225
RsaI GTAC 1 cut(s) 473
RsaNI GTAC 1 cut(s) 472
SatI GCNGC 2 cut(s) 118, 166
Sau3AI GATC 4 cut(s) 96, 225, 280, 385
Sau96I GGNCC 2 cut(s) 546, 639
SchI GAGTC 1 cut(s) 302
ScrFI CCNGG 1 cut(s) 403
SinI GGWCC 1 cut(s) 546
SphI GCATGC 1 cut(s) 196
SsiI CCGC 5 cut(s) 118, 130, 165, 491, 502
SspMI CTAG 2 cut(s) 297, 383
StyD4I CCNGG 1 cut(s) 401
StyI CCWWGG 3 cut(s) 429, 438, 642
TauI GCSGC 2 cut(s) 120, 168
TfiI GAWTC 1 cut(s) 406
TscAI CASTG 3 cut(s) 175, 263, 565
TseFI GTSAC 1 cut(s) 515
Tsp45I GTSAC 1 cut(s) 515
TspDTI ATGAA 2 cut(s) 17, 304
TspRI CASTG 3 cut(s) 175, 263, 565
VpaK11BI GGWCC 1 cut(s) 546
XbaI TCTAGA 1 cut(s) 382
XceI RCATGY 2 cut(s) 196, 400
XmnI GAANNNNTTC 1 cut(s) 8
XspI CTAG 2 cut(s) 297, 383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.