Rorug06G0253700

Protein of unknown function (DUF740)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0014253
Physical Location & Seq
Reverse (-)
40010308 .. 40011513
1206 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0253700.1

Sequence Viewer

Length: 1206 bp
ATGTTGGCTAAACAGGGATGGAGGCTGGTTTCTAACCCACAGTCACTCATTGCCAAGTTGTTCAAGGCTCGATATCATCCTCATTGTTCCTTTTGGGAAGCTGATCTTGGTGAGTCTCCGTCCTTCTCTTGGAGGAGTATACTGCAGGATAGATCGGTGCTCAAGGCCGGCTTACAGTGGCGTGTAGGAGATGGGAAACATGTGCATATATGGCATGACAAATGGATACCGGAGTGTCAACAGTTTCAAGTCCAAAGGCCTCAGGGCTGTGAGTTTGAAATGGTTGAAGAGCTCATTGATGCCAACAACAAAGAATGGAAGACTTCTCTGATTCATGCTACCTTCTCGCCAGTCATAGCTCAAAAAATATTGTGCATACCTCTTAGTCATAGACCAATGGGAGATAATCTGTTATGGAGCGCTAAGAAAAAAGTCTACTCGGTGAAATCTGCCCATTGGGTTGCACGACCCATGGTGTTAGGTAATGCCCTGCCTTCAACCTCTAATGGGGATCCGTTCCAAGTGTTATGGAAAAAGTTGTGGAGAGCTAATGTACCGAGGAAGGTGAAAATATGTCTTTGGAGAGCTTTTAATGACCTCCTGCCTACTCGCATCAAACTGTGTACCAAGGGATTTCAGGGACCAACTGACTGCTCCCTTTGTTCCCATCGTTTTGAAGATACGGCACACATACTATGTAAGTGTCCTGCTGCCTCAGCTATTCTATCTTCCCCTCCTTTTAATCTTGGAGGCAGTTTACTTCCACACATTCAATTCAAGGAGTGGATGCTGGAAAGGGCTATGAGCTTTCATCATGACGCTTTTGATCACCTTCTTATGGTACTATGGGCTTTGTGGAAGAATAGGAATAACAAAGTGTGGAATGGCACTCAAAAATCTGTCAACGACATCTTTCTAAGTGCCACTGAATGGCTTCAGGAGTTTCAACAGGCTAGACAAGTGCCTAAACCTGCTCATGCACGAACTACACAACACTGGAAACCAGCTTTTGAAGGTAAATGGAAATTGAACGTTGATGGAAGTTATCTCCCGAACCAAGTAAAGGGTGGGTTGGGGGGAGTTCTACGTGATGACCTGGGGAATTTTCGGGCGGCATTTGCAATTCCTATTTCTCATGTCAGCTCTGCTAAGCATACTGAGCTGTTCGCTATCAAAGAAGGCCTCAAACTGCTGCAAACATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF740

Protein Analysis

401

Amino Acids

45.86

Weight (kDa)

9.6

Isoelectric Point (pI)

40.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RVT PF13966 174 - 240 1.5e-13 zinc-binding in reverse transcriptase
RVT_3 PF13456 344 - 399 3.1e-06 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014253)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G21830
fragaria_vesca FvH4_2g26860
malus_domestica MD08G1163000.v1.1 MD15G1349700.v1.1
prunus_persica Prupe.1G498100_v2.0.a1
pyrus_communis pycom08g14080 pycom15g31280
rosa_chinensis RchiOBHm_Chr6g0295711
rosa_laevigata RLG00000011773
rosa_multiflora Rmu_sc0007372.1_g000002
rosa_roxburghii Rroxscaffold_7G00172190
rosa_rugosa Rorug06G0253700
rosa_samantha Rh6AG367100 Rh6BG374900 Rh6CG381200 Rh6DG368000
rosa_wichuraiana Rw6G032040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 979
AccB7I CCANNNNNTGG 1 cut(s) 930
AccI GTMKAC 2 cut(s) 139, 435
AciI CCGC 1 cut(s) 1112
AclI AACGTT 1 cut(s) 1032
AclWI GGATC 2 cut(s) 506, 519
AcsI RAATTY 1 cut(s) 1102
AcuI CTGAAG 1 cut(s) 920
AfaI GTAC 3 cut(s) 555, 625, 843
AfeI AGCGCT 1 cut(s) 421
AfiI CCNNNNNNNGG 5 cut(s) 129, 507, 838, 930, 1063
AflIII ACRYGT 1 cut(s) 199
AjnI CCWGG 1 cut(s) 1095
AjuI GAANNNNNNNTTGG 2 cut(s) 90, 122
Alw21I GWGCWC 2 cut(s) 162, 294
Alw26I GTCTC 1 cut(s) 120
AlwI GGATC 2 cut(s) 506, 519
Aor51HI AGCGCT 1 cut(s) 421
AoxI GGCC 3 cut(s) 165, 257, 1180
ApeKI GCWGC 2 cut(s) 710, 1192
ApoI RAATTY 1 cut(s) 1102
Asp700I GAANNNNTTC 1 cut(s) 933
AspLEI GCGC 1 cut(s) 422
AspS9I GGNCC 1 cut(s) 641
AsuHPI GGTGA 4 cut(s) 122, 454, 577, 821
AvaII GGWCC 1 cut(s) 641
AxyI CCTNAGG 1 cut(s) 261
BaeI ACNNNNGTAYC 2 cut(s) 218, 251
BamHI GGATCC 1 cut(s) 511
BanII GRGCYC 1 cut(s) 294
BbsI GAAGAC 1 cut(s) 326
Bbv12I GWGCWC 2 cut(s) 162, 294
BbvCI CCTCAGC 1 cut(s) 715
BbvI GCAGC 2 cut(s) 697, 1179
BccI CCATC 4 cut(s) 12, 185, 675, 1031
BceAI ACGGC 1 cut(s) 699
BciT130I CCWGG 1 cut(s) 1097
BciVI GTATCC 1 cut(s) 219
BclI TGATCA 1 cut(s) 826
BcoDI GTCTC 1 cut(s) 120
BfaI CTAG 1 cut(s) 954
BfmI CTRYAG 1 cut(s) 143
BfoI RGCGCY 1 cut(s) 423
BfuAI ACCTGC 1 cut(s) 979
BfuI GTATCC 1 cut(s) 219
BisI GCNGC 3 cut(s) 711, 1113, 1193
BlpI GCTNAGC 1 cut(s) 1149
BlsI GCNGC 3 cut(s) 712, 1114, 1194
Bme1390I CCNGG 1 cut(s) 1097
Bme18I GGWCC 1 cut(s) 641
BmgT120I GGNCC 1 cut(s) 641
BmiI GGNNCC 2 cut(s) 513, 642
BmrFI CCNGG 1 cut(s) 1097
BmsI GCATC 3 cut(s) 289, 621, 777
BpiI GAAGAC 1 cut(s) 326
Bpu10I CCTNAGC 1 cut(s) 715
Bpu1102I GCTNAGC 1 cut(s) 1149
BpuEI CTTGAG 1 cut(s) 146
BsaAI YACGTR 1 cut(s) 1088
BsaJI CCNNGG 4 cut(s) 471, 557, 627, 1096
BsaWI WCCGGW 1 cut(s) 229
Bsc4I CCNNNNNNNGG 5 cut(s) 129, 507, 838, 930, 1063
Bse118I RCCGGY 1 cut(s) 167
Bse1I ACTGG 2 cut(s) 350, 1001
Bse21I CCTNAGG 1 cut(s) 261
Bse3DI GCAATG 1 cut(s) 48
BseBI CCWGG 1 cut(s) 1097
BseDI CCNNGG 4 cut(s) 471, 557, 627, 1096
BseGI GGATG 3 cut(s) 23, 76, 792
BseLI CCNNNNNNNGG 5 cut(s) 129, 507, 838, 930, 1063
BseMI GCAATG 1 cut(s) 48
BseMII CTCAG 3 cut(s) 275, 729, 1149
BseNI ACTGG 2 cut(s) 350, 1001
BseRI GAGGAG 1 cut(s) 148
BseXI GCAGC 2 cut(s) 697, 1179
BshFI GGCC 3 cut(s) 167, 259, 1182
BsiHKAI GWGCWC 2 cut(s) 162, 294
BsiSI CCGG 2 cut(s) 168, 230
BslFI GGGAC 1 cut(s) 654
BslI CCNNNNNNNGG 5 cut(s) 129, 507, 838, 930, 1063
BsmAI GTCTC 1 cut(s) 120
BsmFI GGGAC 1 cut(s) 654
BsnI GGCC 3 cut(s) 167, 259, 1182
Bsp1286I GDGCHC 2 cut(s) 162, 294
Bsp143I GATC 4 cut(s) 103, 152, 511, 826
Bsp1720I GCTNAGC 1 cut(s) 1149
Bsp19I CCATGG 1 cut(s) 471
BspACI CCGC 1 cut(s) 1112
BspANI GGCC 3 cut(s) 167, 259, 1182
BspCNI CTCAG 3 cut(s) 274, 728, 1150
BspHI TCATGA 1 cut(s) 814
BspLI GGNNCC 2 cut(s) 513, 642
BspMAI CTGCAG 1 cut(s) 147
BspMI ACCTGC 1 cut(s) 979
BspPI GGATC 2 cut(s) 506, 519
BspQI GCTCTTC 1 cut(s) 282
BsrDI GCAATG 1 cut(s) 48
BsrFI RCCGGY 1 cut(s) 167
BsrI ACTGG 2 cut(s) 350, 1001
BssAI RCCGGY 1 cut(s) 167
BssECI CCNNGG 4 cut(s) 471, 557, 627, 1096
BssMI GATC 4 cut(s) 103, 152, 511, 826
BssNAI GTATAC 1 cut(s) 140
BssT1I CCWWGG 2 cut(s) 471, 627
Bst1107I GTATAC 1 cut(s) 140
Bst2UI CCWGG 1 cut(s) 1097
Bst4CI ACNGT 4 cut(s) 42, 177, 243, 621
Bst6I CTCTTC 1 cut(s) 282
BstBAI YACGTR 1 cut(s) 1088
BstC8I GCNNGC 1 cut(s) 169
BstDEI CTNAG 7 cut(s) 261, 383, 423, 715, 917, 1149, 1158
BstDSI CCRYGG 1 cut(s) 471
BstF5I GGATG 3 cut(s) 23, 76, 792
BstH2I RGCGCY 1 cut(s) 423
BstHHI GCGC 1 cut(s) 422
BstKTI GATC 4 cut(s) 106, 155, 514, 829
BstMAI GTCTC 1 cut(s) 120
BstMBI GATC 4 cut(s) 103, 152, 511, 826
BstMWI GCNNNNNNNGC 4 cut(s) 211, 716, 1118, 1159
BstNI CCWGG 1 cut(s) 1097
BstNSI RCATGY 1 cut(s) 203
BstSCI CCNGG 1 cut(s) 1095
BstSFI CTRYAG 1 cut(s) 143
BstV1I GCAGC 2 cut(s) 697, 1179
BstV2I GAAGAC 1 cut(s) 326
BstX2I RGATCY 1 cut(s) 511
BstYI RGATCY 1 cut(s) 511
BstZ17I GTATAC 1 cut(s) 140
Bsu36I CCTNAGG 1 cut(s) 261
BsuI GTATCC 1 cut(s) 219
BsuRI GGCC 3 cut(s) 167, 259, 1182
BtgI CCRYGG 1 cut(s) 471
BtsCI GGATG 3 cut(s) 23, 76, 792
BtsIMutI CAGTG 3 cut(s) 182, 924, 994
BveI ACCTGC 1 cut(s) 979
Cac8I GCNNGC 1 cut(s) 169
CciI TCATGA 1 cut(s) 814
CfoI GCGC 1 cut(s) 422
Cfr10I RCCGGY 1 cut(s) 167
Cfr13I GGNCC 1 cut(s) 641
CseI GACGC 1 cut(s) 827
Csp6I GTAC 3 cut(s) 554, 624, 842
CviAII CATG 7 cut(s) 200, 215, 335, 472, 815, 977, 1136
CviQI GTAC 3 cut(s) 554, 624, 842
DdeI CTNAG 7 cut(s) 261, 383, 423, 715, 917, 1149, 1158
DpnI GATC 4 cut(s) 105, 154, 513, 828
DpnII GATC 4 cut(s) 103, 152, 511, 826
Eam1104I CTCTTC 1 cut(s) 282
EarI CTCTTC 1 cut(s) 282
Ecl136II GAGCTC 1 cut(s) 292
Eco130I CCWWGG 2 cut(s) 471, 627
Eco147I AGGCCT 2 cut(s) 259, 1182
Eco24I GRGCYC 1 cut(s) 294
Eco32I GATATC 1 cut(s) 74
Eco47I GGWCC 1 cut(s) 641
Eco47III AGCGCT 1 cut(s) 421
Eco53kI GAGCTC 1 cut(s) 292
Eco57I CTGAAG 1 cut(s) 920
Eco81I CCTNAGG 1 cut(s) 261
EcoICRI GAGCTC 1 cut(s) 292
EcoRII CCWGG 1 cut(s) 1095
EcoRV GATATC 1 cut(s) 74
EcoT14I CCWWGG 2 cut(s) 471, 627
EcoT38I GRGCYC 1 cut(s) 294
ErhI CCWWGG 2 cut(s) 471, 627
FaeI CATG 7 cut(s) 203, 218, 338, 475, 818, 980, 1139
FalI AAGNNNNNCTT 4 cut(s) 90, 122, 155, 187
FaqI GGGAC 1 cut(s) 654
FatI CATG 7 cut(s) 199, 214, 334, 471, 814, 976, 1135
FbaI TGATCA 1 cut(s) 826
FblI GTMKAC 2 cut(s) 139, 435
Fnu4HI GCNGC 3 cut(s) 711, 1113, 1193
FokI GGATG 3 cut(s) 30, 63, 799
FriOI GRGCYC 1 cut(s) 294
Fsp4HI GCNGC 3 cut(s) 711, 1113, 1193
FspBI CTAG 1 cut(s) 954
GlaI GCGC 1 cut(s) 421
GluI GCNGC 3 cut(s) 711, 1113, 1193
HaeII RGCGCY 1 cut(s) 423
HaeIII GGCC 3 cut(s) 167, 259, 1182
HapII CCGG 2 cut(s) 168, 230
HgaI GACGC 1 cut(s) 827
HhaI GCGC 1 cut(s) 422
Hin1II CATG 7 cut(s) 203, 218, 338, 475, 818, 980, 1139
Hin6I GCGC 1 cut(s) 420
HinP1I GCGC 1 cut(s) 420
HincII GTYRAC 2 cut(s) 239, 904
HindII GTYRAC 2 cut(s) 239, 904
HinfI GANTC 2 cut(s) 113, 331
HpaII CCGG 2 cut(s) 168, 230
HphI GGTGA 4 cut(s) 122, 454, 577, 821
Hpy166II GTNNAC 6 cut(s) 140, 239, 436, 624, 758, 904
Hpy188I TCNGA 1 cut(s) 330
Hpy188III TCNNGA 3 cut(s) 815, 938, 1051
Hpy8I GTNNAC 6 cut(s) 140, 239, 436, 624, 758, 904
HpyAV CCTTC 7 cut(s) 133, 352, 504, 556, 842, 1007, 1172
HpyCH4III ACNGT 4 cut(s) 42, 177, 243, 621
HpyCH4IV ACGT 2 cut(s) 1032, 1087
HpyCH4V TGCA 7 cut(s) 145, 205, 375, 464, 980, 1121, 1195
HpyF10VI GCNNNNNNNGC 4 cut(s) 211, 716, 1118, 1159
HpyF3I CTNAG 7 cut(s) 261, 383, 423, 715, 917, 1149, 1158
HpySE526I ACGT 2 cut(s) 1032, 1087
Hsp92II CATG 7 cut(s) 203, 218, 338, 475, 818, 980, 1139
HspAI GCGC 1 cut(s) 420
KroI GCCGGC 1 cut(s) 167
KroNI GCCGGC 1 cut(s) 169
Ksp22I TGATCA 1 cut(s) 826
Kzo9I GATC 4 cut(s) 103, 152, 511, 826
LguI GCTCTTC 1 cut(s) 282
LmnI GCTCC 2 cut(s) 417, 659
Lsp1109I GCAGC 2 cut(s) 697, 1179
LweI GCATC 3 cut(s) 289, 621, 777
MaeI CTAG 1 cut(s) 954
MaeII ACGT 2 cut(s) 1032, 1087
MaeIII GTNAC 1 cut(s) 42
MalI GATC 4 cut(s) 105, 154, 513, 828
MboI GATC 4 cut(s) 103, 152, 511, 826
MboII GAAGA 5 cut(s) 299, 331, 689, 720, 871
MflI RGATCY 1 cut(s) 511
MhlI GDGCHC 2 cut(s) 162, 294
MluCI AATT 4 cut(s) 773, 1025, 1102, 1122
MlyI GAGTC 1 cut(s) 122
MroNI GCCGGC 1 cut(s) 167
MroXI GAANNNNTTC 1 cut(s) 933
MseI TTAA 2 cut(s) 591, 741
MspI CCGG 2 cut(s) 168, 230
MspR9I CCNGG 1 cut(s) 1097
MvaI CCWGG 1 cut(s) 1097
MwoI GCNNNNNNNGC 4 cut(s) 211, 716, 1118, 1159
NaeI GCCGGC 1 cut(s) 169
NcoI CCATGG 1 cut(s) 471
NdeII GATC 4 cut(s) 103, 152, 511, 826
NgoMIV GCCGGC 1 cut(s) 167
NlaIII CATG 7 cut(s) 203, 218, 338, 475, 818, 980, 1139
NlaIV GGNNCC 2 cut(s) 513, 642
NmuCI GTSAC 1 cut(s) 42
NspI RCATGY 1 cut(s) 203
PagI TCATGA 1 cut(s) 814
PceI AGGCCT 2 cut(s) 259, 1182
PciI ACATGT 1 cut(s) 199
PciSI GCTCTTC 1 cut(s) 282
PdiI GCCGGC 1 cut(s) 169
PdmI GAANNNNTTC 1 cut(s) 933
PfeI GAWTC 1 cut(s) 331
PflMI CCANNNNNTGG 1 cut(s) 930
PkrI GCNGC 3 cut(s) 712, 1114, 1194
PleI GAGTC 1 cut(s) 121
PpsI GAGTC 1 cut(s) 121
Ppu21I YACGTR 1 cut(s) 1088
PscI ACATGT 1 cut(s) 199
Psp124BI GAGCTC 1 cut(s) 294
Psp1406I AACGTT 1 cut(s) 1032
Psp6I CCWGG 1 cut(s) 1095
PspGI CCWGG 1 cut(s) 1095
PspN4I GGNNCC 2 cut(s) 513, 642
PspPI GGNCC 1 cut(s) 641
PstI CTGCAG 1 cut(s) 147
PsuI RGATCY 1 cut(s) 511
RsaI GTAC 3 cut(s) 555, 625, 843
RsaNI GTAC 3 cut(s) 554, 624, 842
SacI GAGCTC 1 cut(s) 294
SapI GCTCTTC 1 cut(s) 282
SaqAI TTAA 2 cut(s) 591, 741
SatI GCNGC 3 cut(s) 711, 1113, 1193
Sau3AI GATC 4 cut(s) 103, 152, 511, 826
Sau96I GGNCC 1 cut(s) 641
SchI GAGTC 1 cut(s) 122
ScrFI CCNGG 1 cut(s) 1097
SduI GDGCHC 2 cut(s) 162, 294
SfaNI GCATC 3 cut(s) 289, 621, 777
SfcI CTRYAG 1 cut(s) 143
SinI GGWCC 1 cut(s) 641
SmlI CTYRAG 1 cut(s) 161
SmoI CTYRAG 1 cut(s) 161
Sse9I AATT 4 cut(s) 773, 1025, 1102, 1122
SseBI AGGCCT 2 cut(s) 259, 1182
SsiI CCGC 1 cut(s) 1112
SspI AATATT 1 cut(s) 369
SspMI CTAG 1 cut(s) 954
SstI GAGCTC 1 cut(s) 294
StuI AGGCCT 2 cut(s) 259, 1182
StyD4I CCNGG 1 cut(s) 1095
StyI CCWWGG 2 cut(s) 471, 627
TaaI ACNGT 4 cut(s) 42, 177, 243, 621
TaiI ACGT 2 cut(s) 1035, 1090
TaqI TCGA 1 cut(s) 70
TasI AATT 4 cut(s) 773, 1025, 1102, 1122
TauI GCSGC 1 cut(s) 1115
TfiI GAWTC 1 cut(s) 331
Tru1I TTAA 2 cut(s) 591, 741
Tru9I TTAA 2 cut(s) 591, 741
TscAI CASTG 3 cut(s) 182, 931, 1001
TseFI GTSAC 1 cut(s) 42
TseI GCWGC 2 cut(s) 710, 1192
Tsp45I GTSAC 1 cut(s) 42
TspDTI ATGAA 2 cut(s) 323, 800
TspGWI ACGGA 2 cut(s) 108, 504
TspRI CASTG 3 cut(s) 182, 931, 1001
Van91I CCANNNNNTGG 1 cut(s) 930
VpaK11BI GGWCC 1 cut(s) 641
XapI RAATTY 1 cut(s) 1102
XceI RCATGY 1 cut(s) 203
XcmI CCANNNNNNNNNTGG 1 cut(s) 1064
XmiI GTMKAC 2 cut(s) 139, 435
XmnI GAANNNNTTC 1 cut(s) 933
XspI CTAG 1 cut(s) 954
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.