AT1G23350

Invertase pectin methylesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0018750
Physical Location & Seq
Reverse (-)
8293020 .. 8294264
1245 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G23350.1

Sequence Viewer

Length: 480 bp
ATGTCTATCAATCCGTATGTGACAATCTTCCTCCTCCTCGCCGCTACCTCAAGCCAAGCGTTGAGTATTTCTCTAGCTGACCCCTGCACGGTGTCGGATTTTCCTGCATTGTGCAGAAGTACCATTAAGGGTCAAACTAATGTGAATGCAGCGACGGATGTGGCCATAAAAGAGTTGATGAAGCGGACGAGGCAAGCCAAGAAAATCGCGGAGAAAGAGCTGAAACGCGATGGAGGAGTCGCGACTTGTTTGTCCAACTTCAATAGCGCGTTTGATAATTTGGAAAAGGCTCTTACAAACATTAAGGAAAATGATGGTTTTAGCCTTAACATTAACCTTAGCGCCGCCTTGACGGATTACGATACTTGCAGCGACTCGATGAAGGAAACTAAGGAGGTCAACGTCATTTATAAATCCGCCGGGGTTTTGTACCGTATGGCTGATAATTGCTTGGCGCTTTCAACTCTCGTTAAACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

159

Amino Acids

17.26

Weight (kDa)

7.57

Isoelectric Point (pI)

29.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 29 - 153 1e-17 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018750)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10770 AT1G23350 AT1G60760 AT1G70540
prunus_persica Prupe.1G249100_v2.0.a1
rosa_multiflora Rmu_sc0004275.1_g000005
rosa_samantha Rh4BG328900 Rh4CG344200 Rh4DG325600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 411
AasI GACNNNNNNGTC 1 cut(s) 250
AccII CGCG 4 cut(s) 209, 228, 242, 269
AciI CCGC 5 cut(s) 42, 184, 209, 345, 417
AcoI YGGCCR 1 cut(s) 162
AfaI GTAC 2 cut(s) 121, 431
AfiI CCNNNNNNNGG 1 cut(s) 88
AgsI TTSAA 2 cut(s) 262, 462
AluBI AGCT 2 cut(s) 77, 220
AluI AGCT 2 cut(s) 77, 220
AoxI GGCC 1 cut(s) 162
ApeKI GCWGC 2 cut(s) 149, 369
AspLEI GCGC 3 cut(s) 269, 344, 457
AsuC2I CCSGG 1 cut(s) 421
BalI TGGCCA 1 cut(s) 164
BbvI GCAGC 2 cut(s) 161, 381
BccI CCATC 2 cut(s) 224, 308
BcnI CCSGG 1 cut(s) 421
BfaI CTAG 1 cut(s) 74
BfoI RGCGCY 2 cut(s) 345, 458
BisI GCNGC 4 cut(s) 42, 150, 345, 370
BlsI GCNGC 4 cut(s) 43, 151, 346, 371
Bme1390I CCNGG 1 cut(s) 421
BmrFI CCNGG 1 cut(s) 421
BplI GAGNNNNNCTC 2 cut(s) 55, 87
Bpu10I CCTNAGC 1 cut(s) 338
BpuEI CTTGAG 1 cut(s) 34
BpuMI CCSGG 1 cut(s) 421
BsaJI CCNNGG 1 cut(s) 420
Bsc4I CCNNNNNNNGG 1 cut(s) 88
BseDI CCNNGG 1 cut(s) 420
BseGI GGATG 1 cut(s) 163
BseLI CCNNNNNNNGG 1 cut(s) 88
BseRI GAGGAG 3 cut(s) 23, 26, 249
BseXI GCAGC 2 cut(s) 161, 381
BsgI GTGCAG 2 cut(s) 70, 133
Bsh1236I CGCG 4 cut(s) 209, 228, 242, 269
BshFI GGCC 1 cut(s) 164
BsiSI CCGG 1 cut(s) 420
BslI CCNNNNNNNGG 1 cut(s) 88
BsmI GAATGC 1 cut(s) 151
BsnI GGCC 1 cut(s) 164
Bsp68I TCGCGA 1 cut(s) 242
BspACI CCGC 5 cut(s) 42, 184, 209, 345, 417
BspANI GGCC 1 cut(s) 164
BspFNI CGCG 4 cut(s) 209, 228, 242, 269
BssECI CCNNGG 1 cut(s) 420
Bst4CI ACNGT 2 cut(s) 91, 434
BstC8I GCNNGC 1 cut(s) 195
BstDEI CTNAG 2 cut(s) 338, 390
BstF5I GGATG 1 cut(s) 163
BstFNI CGCG 4 cut(s) 209, 228, 242, 269
BstH2I RGCGCY 2 cut(s) 345, 458
BstHHI GCGC 3 cut(s) 269, 344, 457
BstMWI GCNNNNNNNGC 1 cut(s) 190
BstSCI CCNGG 1 cut(s) 419
BstUI CGCG 4 cut(s) 209, 228, 242, 269
BstV1I GCAGC 2 cut(s) 161, 381
BsuRI GGCC 1 cut(s) 164
BtgZI GCGATG 1 cut(s) 243
BtsCI GGATG 1 cut(s) 163
BtuMI TCGCGA 1 cut(s) 242
Cac8I GCNNGC 1 cut(s) 195
CfoI GCGC 3 cut(s) 269, 344, 457
Csp6I GTAC 2 cut(s) 120, 430
CviJI RGCY 8 cut(s) 54, 77, 164, 197, 220, 290, 324, 440
CviKI_1 RGCY 8 cut(s) 54, 77, 164, 197, 220, 290, 324, 440
CviQI GTAC 2 cut(s) 120, 430
DdeI CTNAG 2 cut(s) 338, 390
DrdI GACNNNNNNGTC 1 cut(s) 250
DseDI GACNNNNNNGTC 1 cut(s) 250
EaeI YGGCCR 1 cut(s) 162
EciI GGCGGA 1 cut(s) 406
FaiI YATR 4 cut(s) 18, 167, 411, 437
Fnu4HI GCNGC 4 cut(s) 42, 150, 345, 370
FokI GGATG 1 cut(s) 170
Fsp4HI GCNGC 4 cut(s) 42, 150, 345, 370
FspBI CTAG 1 cut(s) 74
GlaI GCGC 3 cut(s) 268, 343, 456
GluI GCNGC 4 cut(s) 42, 150, 345, 370
HaeII RGCGCY 2 cut(s) 345, 458
HaeIII GGCC 1 cut(s) 164
HapII CCGG 1 cut(s) 420
HhaI GCGC 3 cut(s) 269, 344, 457
Hin6I GCGC 3 cut(s) 267, 342, 455
HinP1I GCGC 3 cut(s) 267, 342, 455
HincII GTYRAC 1 cut(s) 400
HindII GTYRAC 1 cut(s) 400
HinfI GANTC 2 cut(s) 237, 374
HpaII CCGG 1 cut(s) 420
Hpy166II GTNNAC 1 cut(s) 400
Hpy188I TCNGA 1 cut(s) 97
Hpy188III TCNNGA 1 cut(s) 241
Hpy8I GTNNAC 1 cut(s) 400
Hpy99I CGWCG 1 cut(s) 157
HpyAV CCTTC 1 cut(s) 376
HpyCH4III ACNGT 2 cut(s) 91, 434
HpyCH4IV ACGT 1 cut(s) 402
HpyCH4V TGCA 5 cut(s) 87, 107, 114, 149, 369
HpyF10VI GCNNNNNNNGC 1 cut(s) 190
HpyF3I CTNAG 2 cut(s) 338, 390
HpySE526I ACGT 1 cut(s) 402
HspAI GCGC 3 cut(s) 267, 342, 455
LpnPI CCDG 3 cut(s) 97, 117, 433
Lsp1109I GCAGC 2 cut(s) 161, 381
MaeI CTAG 1 cut(s) 74
MaeII ACGT 1 cut(s) 402
MaeIII GTNAC 1 cut(s) 19
MboII GAAGA 1 cut(s) 19
MlsI TGGCCA 1 cut(s) 164
MluCI AATT 2 cut(s) 277, 445
MluNI TGGCCA 1 cut(s) 164
MlyI GAGTC 2 cut(s) 246, 368
MmeI TCCRAC 2 cut(s) 75, 279
MnlI CCTC 7 cut(s) 41, 44, 47, 58, 183, 227, 388
Mox20I TGGCCA 1 cut(s) 164
MscI TGGCCA 1 cut(s) 164
MseI TTAA 5 cut(s) 126, 303, 327, 333, 471
Msp20I TGGCCA 1 cut(s) 164
MspI CCGG 1 cut(s) 420
MspR9I CCNGG 1 cut(s) 421
Mva1269I GAATGC 1 cut(s) 151
MvnI CGCG 4 cut(s) 209, 228, 242, 269
MwoI GCNNNNNNNGC 1 cut(s) 190
NciI CCSGG 1 cut(s) 421
NmuCI GTSAC 1 cut(s) 19
NruI TCGCGA 1 cut(s) 242
PctI GAATGC 1 cut(s) 151
PkrI GCNGC 4 cut(s) 43, 151, 346, 371
PleI GAGTC 2 cut(s) 245, 368
PpsI GAGTC 2 cut(s) 245, 368
PsiI TTATAA 1 cut(s) 411
RruI TCGCGA 1 cut(s) 242
RsaI GTAC 2 cut(s) 121, 431
RsaNI GTAC 2 cut(s) 120, 430
SaqAI TTAA 5 cut(s) 126, 303, 327, 333, 471
SatI GCNGC 4 cut(s) 42, 150, 345, 370
SchI GAGTC 2 cut(s) 246, 368
ScrFI CCNGG 1 cut(s) 421
SetI ASST 6 cut(s) 50, 79, 222, 339, 399, 405
SmlI CTYRAG 1 cut(s) 49
SmoI CTYRAG 1 cut(s) 49
Sse9I AATT 2 cut(s) 277, 445
SsiI CCGC 5 cut(s) 42, 184, 209, 345, 417
SspMI CTAG 1 cut(s) 74
StyD4I CCNGG 1 cut(s) 419
TaaI ACNGT 2 cut(s) 91, 434
TaiI ACGT 1 cut(s) 405
TaqI TCGA 1 cut(s) 377
TasI AATT 2 cut(s) 277, 445
TauI GCSGC 2 cut(s) 44, 347
Tru1I TTAA 5 cut(s) 126, 303, 327, 333, 471
Tru9I TTAA 5 cut(s) 126, 303, 327, 333, 471
TseFI GTSAC 1 cut(s) 19
TseI GCWGC 2 cut(s) 149, 369
Tsp45I GTSAC 1 cut(s) 19
TspDTI ATGAA 2 cut(s) 194, 395
TspGWI ACGGA 2 cut(s) 170, 368
XspI CTAG 1 cut(s) 74
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.