Rh4DG325600

Plant invertase/pectin methylesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0018750
Physical Location & Seq
Forward (+)
55007271 .. 55007522
252 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG325600.1

Sequence Viewer

Length: 252 bp
ATGGCCACCGCGTTATTCCATGAGTCGAGCACCGAGCTTTGCGCCGGAGCCGACTACAAAACCCTCTGTCGGTCCATCGGGGGAAGAGCCAAAACCCCATACGCGGCGGCGGAGGCCATCGTCAAGCGACTCACTTCCGAGACCAAGAAAGCAAAAGCGTCCGCCGCTAAGCTCCCCAAAAGGACGCCTGAGTTTGCACGTCTCTGTATAGAGACGCCATTACCGACCTGGAGACGAGCGTGGAAGATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

83

Amino Acids

9.14

Weight (kDa)

9.96

Isoelectric Point (pI)

49.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018750)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10770 AT1G23350 AT1G60760 AT1G70540
prunus_persica Prupe.1G249100_v2.0.a1
rosa_multiflora Rmu_sc0004275.1_g000005
rosa_samantha Rh4BG328900 Rh4CG344200 Rh4DG325600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 11, 104
AciI CCGC 6 cut(s) 9, 104, 107, 110, 162, 165
AcoI YGGCCR 1 cut(s) 3
AcyI GRCGYC 2 cut(s) 185, 215
AfiI CCNNNNNNNGG 2 cut(s) 69, 103
AjiI CACGTC 1 cut(s) 200
AjnI CCWGG 1 cut(s) 227
AluBI AGCT 2 cut(s) 37, 172
AluI AGCT 2 cut(s) 37, 172
Alw21I GWGCWC 1 cut(s) 32
Alw26I GTCTC 4 cut(s) 134, 206, 206, 226
AoxI GGCC 2 cut(s) 3, 114
AspLEI GCGC 1 cut(s) 44
AspS9I GGNCC 1 cut(s) 72
AvaII GGWCC 1 cut(s) 72
BalI TGGCCA 1 cut(s) 5
Bbv12I GWGCWC 1 cut(s) 32
BccI CCATC 2 cut(s) 83, 125
BciT130I CCWGG 1 cut(s) 229
BcoDI GTCTC 4 cut(s) 134, 206, 206, 226
BisI GCNGC 3 cut(s) 105, 108, 165
BlpI GCTNAGC 1 cut(s) 168
BlsI GCNGC 3 cut(s) 106, 109, 166
Bme1390I CCNGG 1 cut(s) 229
Bme18I GGWCC 1 cut(s) 72
BmgBI CACGTC 1 cut(s) 200
BmgT120I GGNCC 1 cut(s) 72
BmiI GGNNCC 1 cut(s) 49
BmrFI CCNGG 1 cut(s) 229
BpmI CTGGAG 1 cut(s) 250
Bpu1102I GCTNAGC 1 cut(s) 168
BsaHI GRCGYC 2 cut(s) 185, 215
BsaI GGTCTC 1 cut(s) 134
Bsc4I CCNNNNNNNGG 2 cut(s) 69, 103
BseBI CCWGG 1 cut(s) 229
BseLI CCNNNNNNNGG 2 cut(s) 69, 103
BseMII CTCAG 1 cut(s) 180
Bsh1236I CGCG 2 cut(s) 11, 104
BshFI GGCC 2 cut(s) 5, 116
BsiHKAI GWGCWC 1 cut(s) 32
BsiSI CCGG 1 cut(s) 45
BslI CCNNNNNNNGG 2 cut(s) 69, 103
BsmAI GTCTC 4 cut(s) 134, 206, 206, 226
BsmBI CGTCTC 3 cut(s) 206, 206, 226
BsnI GGCC 2 cut(s) 5, 116
Bso31I GGTCTC 1 cut(s) 134
Bsp1286I GDGCHC 1 cut(s) 32
Bsp1720I GCTNAGC 1 cut(s) 168
BspACI CCGC 6 cut(s) 9, 104, 107, 110, 162, 165
BspANI GGCC 2 cut(s) 5, 116
BspCNI CTCAG 1 cut(s) 181
BspFNI CGCG 2 cut(s) 11, 104
BspLI GGNNCC 1 cut(s) 49
BspQI GCTCTTC 1 cut(s) 79
BspTNI GGTCTC 1 cut(s) 134
BssNI GRCGYC 2 cut(s) 185, 215
Bst2UI CCWGG 1 cut(s) 229
Bst6I CTCTTC 1 cut(s) 79
BstACI GRCGYC 2 cut(s) 185, 215
BstDEI CTNAG 2 cut(s) 168, 189
BstFNI CGCG 2 cut(s) 11, 104
BstHHI GCGC 1 cut(s) 44
BstMAI GTCTC 4 cut(s) 134, 206, 206, 226
BstMWI GCNNNNNNNGC 2 cut(s) 113, 164
BstNI CCWGG 1 cut(s) 229
BstSCI CCNGG 1 cut(s) 227
BstUI CGCG 2 cut(s) 11, 104
BsuRI GGCC 2 cut(s) 5, 116
BtrI CACGTC 1 cut(s) 200
CfoI GCGC 1 cut(s) 44
Cfr13I GGNCC 1 cut(s) 72
CseI GACGC 3 cut(s) 147, 193, 223
CviAII CATG 1 cut(s) 20
CviJI RGCY 6 cut(s) 5, 37, 50, 89, 116, 172
CviKI_1 RGCY 6 cut(s) 5, 37, 50, 89, 116, 172
DdeI CTNAG 2 cut(s) 168, 189
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 79
EarI CTCTTC 1 cut(s) 79
EciI GGCGGA 2 cut(s) 125, 151
Eco31I GGTCTC 1 cut(s) 134
Eco47I GGWCC 1 cut(s) 72
EcoRII CCWGG 1 cut(s) 227
Esp3I CGTCTC 3 cut(s) 206, 206, 226
FaeI CATG 1 cut(s) 23
FaiI YATR 4 cut(s) 21, 100, 209, 250
FatI CATG 1 cut(s) 19
Fnu4HI GCNGC 3 cut(s) 105, 108, 165
Fsp4HI GCNGC 3 cut(s) 105, 108, 165
GlaI GCGC 1 cut(s) 43
GluI GCNGC 3 cut(s) 105, 108, 165
GsuI CTGGAG 1 cut(s) 250
HaeIII GGCC 2 cut(s) 5, 116
HapII CCGG 1 cut(s) 45
HgaI GACGC 3 cut(s) 147, 193, 223
HhaI GCGC 1 cut(s) 44
Hin1I GRCGYC 2 cut(s) 185, 215
Hin1II CATG 1 cut(s) 23
Hin6I GCGC 1 cut(s) 42
HinP1I GCGC 1 cut(s) 42
HinfI GANTC 2 cut(s) 23, 129
HpaII CCGG 1 cut(s) 45
Hpy188I TCNGA 1 cut(s) 139
HpyCH4IV ACGT 1 cut(s) 199
HpyCH4V TGCA 1 cut(s) 197
HpyF10VI GCNNNNNNNGC 2 cut(s) 113, 164
HpyF3I CTNAG 2 cut(s) 168, 189
HpySE526I ACGT 1 cut(s) 199
Hsp92I GRCGYC 2 cut(s) 185, 215
Hsp92II CATG 1 cut(s) 23
HspAI GCGC 1 cut(s) 42
LguI GCTCTTC 1 cut(s) 79
LmnI GCTCC 2 cut(s) 47, 177
LpnPI CCDG 4 cut(s) 58, 201, 214, 241
MaeII ACGT 1 cut(s) 199
MboII GAAGA 1 cut(s) 96
MhlI GDGCHC 1 cut(s) 32
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 32, 123
MnlI CCTC 2 cut(s) 74, 106
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 45
MspR9I CCNGG 1 cut(s) 229
MvaI CCWGG 1 cut(s) 229
MvnI CGCG 2 cut(s) 11, 104
MwoI GCNNNNNNNGC 2 cut(s) 113, 164
NlaIII CATG 1 cut(s) 23
NlaIV GGNNCC 1 cut(s) 49
PciSI GCTCTTC 1 cut(s) 79
PcsI WCGNNNNNNNCGW 1 cut(s) 221
PkrI GCNGC 3 cut(s) 106, 109, 166
PleI GAGTC 2 cut(s) 31, 123
PpsI GAGTC 2 cut(s) 31, 123
Psp6I CCWGG 1 cut(s) 227
PspGI CCWGG 1 cut(s) 227
PspN4I GGNNCC 1 cut(s) 49
PspPI GGNCC 1 cut(s) 72
SapI GCTCTTC 1 cut(s) 79
SatI GCNGC 3 cut(s) 105, 108, 165
Sau96I GGNCC 1 cut(s) 72
SchI GAGTC 2 cut(s) 32, 123
ScrFI CCNGG 1 cut(s) 229
SduI GDGCHC 1 cut(s) 32
SetI ASST 4 cut(s) 39, 174, 202, 230
SinI GGWCC 1 cut(s) 72
SsiI CCGC 6 cut(s) 9, 104, 107, 110, 162, 165
StyD4I CCNGG 1 cut(s) 227
TaiI ACGT 1 cut(s) 202
TaqI TCGA 1 cut(s) 26
TaqII GACCGA 1 cut(s) 60
TauI GCSGC 3 cut(s) 107, 110, 167
VpaK11BI GGWCC 1 cut(s) 72
XcmI CCANNNNNNNNNTGG 1 cut(s) 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.