Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0003911

Physical Location & Seq

Genomic Coordinates Forward (+)

8741836 .. 8755604

Gene Structure 13769 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G24706.5

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5415 bp

ATGTCTCTTCCGCTACTGGAGTGTAAGTACGTGACGGAGGAGTTTGTTCGTGAAGGTAAGAATGGCAACTATGGAACCAAGCTGCCCAGCTCTGTGCCGATGCTTCGTTTCCTCTACGAGCTCTCTTGGATTTTGGTTCGTGGCGAATTGCCAATTCAGAGCTGTAAGGCAGTGTTGGAAGGTGTGGAGTTTTTGGATAAGCCATCGAGAGAAGAATTAGCTTCGTGTTTTGCGGATGTTGTGACTCAGATAGCTCAAGACCTTACCATGTCGGGAGATCAACGTTCTCGTCTAATCAAATTGGCGAAATGGCTTGTGGAATCACAGACAGTCCCTCAAAGGCTTTTCCAAGAGCGTTGCGAGGAGGAGTTTCTCTGGGAAGCTGATATGGTTAAAATAAAGGCCCAAGATTTGAAGGGGAAAGAGGTGCGATTAAACACTCGTCTTCTGTACCAGCAGACAAAGTTCAACTTACTCCGTGAAGAAAGCGAGGGATATGCCAAATTGGCCACTCTTTTGTGCCGAGGATCTGCAAGTTCTTCGCACAATGCATCAGCAGCAACGATGGGAATTATCAAGTCATTAATTGGGCATTTTGACCTGGATCCAAACCGTGTTTTCGACATTGTTTTAGATTGCTTTGAACTCGAACAAGATTACGACACATTTCTCAATCTAATTCCTATCTTTCCCAAGTCTCATGCTTCACAAATTCTTGGATTTAAATTCCAATACTATCAGAGACTAGAGGTGAATAGCCCTGTTCCAGTTGGGCTCTATAAGCTTACAGCCTTGCTAGTAAAGGAAGAGTTTATCAATCTTGAAAGCATATATGCACATCTGCTACCTAAGGACGAGGAAGTTTTTGAGGATTATAATGTTTCTTCTGCAAAGCGGTTTGAAGAGGCCAATAAAATTGGAAAGATTAATCTCGCTGCTACTGGGAAGGATCTTATGGAAGATGAGAAGCAAGGAGATGTCACAGTAGATCTCTTTGCTGCTTTAGACATGGAAAGTGAAGCTGTTACTGAGCGATTACCAGAGCTTGAAAATAATCAGACTCTGGGCCTACTTAATGGTTTCCTCTCTGTGGACGATTGGTATCATGCAAATATCTTGTTCGAGCGTCTTGCACCTCTTAATCCAGTGGCACACGATCAGATCTGTAGTGGGTTGTTTAGGCTTATAGAGAAGTCAATCACTCATTCTTATCGCATTGCTCGTCAGACACGTTTTCAAAGTTCTTCTTCTGCGAGCACAGTTAAGCTCACACCCACTGCTAATACGACAGCTAATAGGACTTATCTAGATCTTCCGAAAGAAGTGTTTCAAATGCTTGTTACTGTTGGACCCTACCTCTACCGCAATACACAATTGTTGCAGAAGATTTGTAGAGTATTAAGGGCTTACTACTTGTCGGCCTTGGATCTTGTTCGCGATGGGTCAAATCAAGAAGGTAGTGCATATGAAGTTTCTCGTGGGCACCTGAAAGAAGTTAGGTTGAGAGTGGAAGAAGCTCTAGGAACATGTCTTCTGCCATCGTTACAGTTAGTACCTGCAAATCCAGCAGTTGGTCATGAGATTTGGGAAGTGATGAGTCTCCTTCCATATGAGGCTCGTTATCGCTTATATGGTGAGTGGGAAAAGGACGATGAGCAGAATCCTTTGTTGTTGGCAGCAAGGCAAGTGGCAAAGTTGGATACTAGAAGAATTCTGAAACGGCTTGCAAAAGAAAATCTGAAGCAGTTAGGAAGGATGGTGGCAAAACTAGCTCATGCCAATCCCATGACGGTGCTTCGGACAATTGTAAATCAGATTGAAGCATACAGAGATATGATTGCTCCTGTTGTGGATGCCTTCAAGTACTTGACACAGCTTGAGTATGATATCCTGGAATATGTAGTGATTGAGAGACTGGCACAAAGCGGACGAGATAAACTTAAAGACGACGGGATTAATTTATCAGACTGGCTTCAATCTTTGGCATCCTTTTGGGGTCACCTGTGTAAGAAGTATCCATCAATGGAGTTGAGGGGTCTTTTTCAGTATCTCGTAAACCAGTTAAAGAGGGGCCAAGGGATTGAGCTTGTTCTTCTCCAGGAACTGGTCCAGCAGATGGCAAATGTACAATACACTGAGAACCTTACTGAAGATCAGTTGGATGCCATGGCAGGAAGTGAGACTTTACGTTATCATGCAACATCATTTGGCATGATGCGAAACAACAAGGCACTTATCAAATCATCAAACCGTCTACGAGATTCGTTACTTCCAAATGATGAACCAAAGTTAGCTATCCCCCTTTTGTTACTTATCGCGCAGCATCGTTCTCTAGTTGTGGTGAATGCGGATGCGCCTTATATTAAGATGGTCACTGAACAGTTCGACAGATGTCATGGAATTCTTCTCCAATACGTGGACTTCCTTTCTAGTGCTGTCAGTCCTACTACTGCTTATGCTCGGCTGGTACCTTCGCTTGATGAGCTTGTTCATACATACCATCTCGAAGCAGAGGTCGCATTTTTGGTATTTCGCCCTGTGATGAGGCTCTTCAAGTGTCGACGGAATGGTGATGTTTCATGGCCTTTAGATAGTGGGGAATCTATGGATGCGGATTCTGAAATATCAGAGTCTGAGAGTTCCATGATTCTCGATGTCGGTACATCTGAAAAAGCTGTAACGTGGTCTGATGTTCTTGATACAGTAAGGACAATGCTGCCCTCAAAAGCTTGGAATAGTCTATCGCCAGACCTCTACGCTACATTTTGGGGGCTTACGCTTTATGATCTCCATGTCCCCAGAAATCGTTATGAGTCTGAGATATCTAAACAGCATACTGCCCTTAAGACACTTGAAGAGGTTGCTGATAATTCTAGCTCAGCAATTACCAAGAGGAAGAAAGAGAAGGAAAGGATTCAAGAGTCTCTTGATCGTCTAACCGGTGAGCTGAAAAAGCATGAAGAACATGTGGCTTCTGTCCGTAGAAGGCTTTCACGTGAAAAGGATACATGGTTGAGCTCCTGTCCAGATACTTTGAAGATTAATATGGAGTTCCTGCAGCGTTGTATTTTCCCTCGTTGCACATTTAGCATGGCAGATTCTGTGTACTGCGCGATGTTTGTAAATATGCTTCACTCGCTTGGAACACCCTTTTTTAACACTGTCAACCACATTGATGTCCTGATATGTAAAACGCTGCAACCAATGATCTGCTGCTGTACTGAATATGAAGTTGGAAGACTGGGGAGGTTTTTGTTTGAGACCTTGAAGATTGCATACCACTGGAAGAGTAAAGAATCAGTCTATGAACATGAGTGCGGAAACATGCCAGGGTTTGCTGTTTATTATAGATACCCGAACAGCCAGCGTGTCACATTTGGACAATTTGTGAAGGTACATTGGAAATGGAGTGGAAGAATCACACGACTTTTGATCCAGTGTCTGGAGTCTAATGAGTATATGGAGATTCGTAATGCCCTTATTATGTTAACAAAAATATCTGGTGTTTTCCCAGTTACTCGGAAAACTGGGATAAACCTTGAGAAACGGGCAACCAAGATTAAAAATGATGAGCGAGAGGACCTTAAGGTCTTAGCAACTGGTGTTGGGGCTGCTCTGTCTGCAAGAAAACCACATTGGGTTACAGACGAAGAATTTAGCATGGGCTTCCTTGAACTAAAGGCTCCGCCAGTACATACTCCAAAACATGCTTCTTCCCAGAATGGCTTGTTAGTTGGTGTCTCCCAAGGTGAACCAACTGGGGAAAGAGCTACTGTTAACCAGCAGCCTGAGTCTGGTGGATTGGGCAAGGACCAGATGTTGAAAACTAAACCTCTAGATGGAAGAACGGAAAGTATACCTTCAAAATCTGATCAAGGACATCTAAAATCAAAGGGAGGCAATCCATTGGATTCCCAACCATCCATATCTAAGAAGTCAATGGAACAAAAGGAAACAGATGAAACACCCAGAATATCTGACGAGAATCCTGTCAAACCTGCTTCAAAATATTCTGAAGCCGAGTTAAAAGCTTCCTCTAAACGAGGTGCGTCAGTGAACAAATCTGCAAAGCAAGATTTTGGAAAAGATGATGGAAAGTCCGGTAAAGCTATTGGAAGAACATCCACAGCTGACAAAGATCTTAATTATCTGGAGAGCAGGCAATCAGGTTTGACCAAAGCTCTTTCCAGCACAGCAGCCAATGGTAGCATAGCTACTGGGTCGTCCAAAGTAAAAGATGATGGCGCTGAAGCTTTGGATGCACAAAAGCAATCTTCTCGGACTGTTCATTCCCCTAGGCATGAAATTGTCACTTCTGTGAGATCCAGTGATAGGTTACAAAAACGAGCTAATGCTGTTGAAGATAGTGAAAGGATAAGTAAACGTCGTAAAGGAGATGCTGAGCACAAAGAGCATGACAGTGAGCCTCGATCGAGTGACCGAGATAGATCAGTCGAGGCCCGATTGGATTTAAACAAAACAGTTACTGATGACCAAAGCACCCATAGGGATCAAGATAGATCAAAGGATAAGGGTTATGAAAGACAGGATCGAGACCACAGAGAAAGAGTCGATCGTTCTGATAAACCTCGTGGAGATGATGTTGAGAAAGCACGAGACAAATCCTTGGAACGTCATGGTAGAGAACGCTCAGTTGAGAAGGGTCTAGATAAAGGAACAACTAGGAGTTATGATAGAAACAAGGATGAAAGAAACAAAGATGATAGAAGCAAACTGCGTCATAGTGAAGCATCACTTGAGAAATCTCATCCTGATGACCATTTTCATTCTCAAGGTTTACCCCCACCTCCACCTTTACCTCCTAACATCATTCCACATTCTATGGCCGCAAAAGAAGACCTCGAAAGAAGGGCTGGCGGTGCAAGGCATTCTCAGAGGCTTTCCCCGAGGCATGAAGAAAGAGAGAAACGACGCTCTGAAGAGAATTTATCAGTTTCTGTGGATGATGCAAAACGTAGAAGAGATGATGATATTAGGGACCGAAAGCGAGATGACCGAGAAACAATCACTGTGAAGGGAGAAGAAAGAGAAAGGGAAAGGGAAAGAGAGAGGGAAAGAGAAAAAAGCCTCCCTTTGAAAGAGGATTTTGAAGCCTCCAAGAGAAGGAAGCTTAAGAGGGAGCAACAGGTCCCATCTGCAGAGCCAGGAGAATACTCTCCTATGCCACATCATTCGTCTTTATCGACCAGTATGGGACCATCATCGTACGAAGGCAGAGAGCGGAAGAGCAGTAGTATGATTCAACACGGAGGTTATCTTGAAGAGCCAAGCATCAGACTTCTTGGAAAAGAAGCTTCCAGCAAAATGGCTCGTCGTGATCCTGACCCAATCTATGACCGTGAATGGGAAGACGACAAGAGGAGAGCAGAGCGGAAGCGGAGAGATCGGAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000346 GO:0000347 GO:0000445 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006396 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0008104 GO:0008150 GO:0008152 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0015031 GO:0015833 GO:0015931 GO:0016070 GO:0016441 GO:0016458 GO:0016604 GO:0016607 GO:0019222 GO:0031047 GO:0031050 GO:0031503 GO:0031974 GO:0031981 GO:0032991 GO:0033036 GO:0034613 GO:0034641 GO:0035194 GO:0035195 GO:0035196 GO:0040029 GO:0042221 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048519 GO:0050657 GO:0050658 GO:0050789 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070727 GO:0070887 GO:0070918 GO:0071166 GO:0071310 GO:0071359 GO:0071407 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1990428

KEGG Pathways

Metabolic & Signaling

ko03013 ko03040 map03013 map03040

Pfam Domains

Protein Families

THOC2_N Tho2 Thoc2

Protein Analysis

1804

Amino Acids

205.72

Weight (kDa)

7.58

Isoelectric Point (pI)

46.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
THOC2_N	PF16134	37 - 420	6.6e-50	THO complex subunit 2 N-terminus
THOC2_N	PF16134	434 - 585	5.7e-20	THO complex subunit 2 N-terminus
Thoc2	PF11732	587 - 662	6.1e-30	Transcription- and export-related complex subunit
Tho2	PF11262	911 - 1204	4.9e-98	Transcription factor/nuclear export subunit protein 2

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0003911)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G24706 AT1G24706 AT1G24706 AT1G24706 AT1G24706 AT1G24706 AT1G24706 AT1G24706 AT1G24706
fragaria_vesca	FvH4_4g33200 FvH4_4g33200 FvH4_4g33200
malus_domestica	MD09G1258800.v1.1
prunus_persica	Prupe.3G150800_v2.0.a1 Prupe.3G150800_v2.0.a1 Prupe.3G150800_v2.0.a1 Prupe.3G150800_v2.0.a1 Prupe.3G150800_v2.0.a1 Prupe.3G150800_v2.0.a1 Prupe.3G150800_v2.0.a1 Prupe.3G150800_v2.0.a1 Prupe.3G150800_v2.0.a1
pyrus_communis	pycom09g17360
rosa_chinensis	RchiOBHm_Chr4g0442311
rosa_laevigata	RLG00000006018
rosa_multiflora	Rmu_sc0000124.1_g000055
rosa_roxburghii	Rroxscaffold_5G00383170
rosa_rugosa	Rorug04G0340300 Rorug04G0340400
rosa_samantha	Rh4AG391900 Rh4BG405400 Rh4CG420600 Rh4DG398700
rosa_wichuraiana	Rw4G033850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	876
AasI	GACNNNNNNGTC	1 cut(s)	3587
Acc36I	ACCTGC	2 cut(s)	1564, 4006
Acc65I	GGTACC	1 cut(s)	2467
AccB1I	GGYRCC	2 cut(s)	1482, 2467
AccB7I	CCANNNNNTGG	5 cut(s)	1571, 2104, 2116, 2416, 3442
AccBSI	CCGCTC	2 cut(s)	5242, 5392
AccI	GTMKAC	3 cut(s)	2254, 2560, 3856
AccII	CGCG	3 cut(s)	1437, 2318, 3113
AclI	AACGTT	1 cut(s)	283
AcoI	YGGCCR	2 cut(s)	507, 4845
AcsI	RAATTY	6 cut(s)	711, 725, 1710, 2400, 3653, 4945
AcuI	CTGAAG	5 cut(s)	1760, 2169, 4035, 4269, 4959
AcvI	CACGTG	1 cut(s)	2996
AflII	CTTAAG	3 cut(s)	2843, 3584, 5132
AflIII	ACRYGT	3 cut(s)	1229, 1526, 2965
AgeI	ACCGGT	1 cut(s)	2939
AjnI	CCWGG	5 cut(s)	600, 1890, 2097, 3328, 5164
AjuI	GAANNNNNNNTTGG	6 cut(s)	3216, 3248, 3619, 3651, 3694, 3726
AleI	CACNNNNGTG	1 cut(s)	4316
Alw21I	GWGCWC	4 cut(s)	123, 1259, 3020, 4407
AlwNI	CAGNNNCTG	7 cut(s)	1063, 2104, 2633, 3101, 3442, 4487, 4958
Ama87I	CYCGRG	1 cut(s)	4906
AoxI	GGCC	9 cut(s)	402, 507, 906, 1066, 1419, 2071, 2582, 4458, 4845
ApoI	RAATTY	6 cut(s)	711, 725, 1710, 2400, 3653, 4945
ArsI	GACNNNNNNTTYG	2 cut(s)	2499, 2531
AseI	ATTAAT	4 cut(s)	584, 927, 1956, 3042
AsiGI	ACCGGT	1 cut(s)	2939
Asp700I	GAANNNNTTC	3 cut(s)	1326, 2913, 2989
Asp718I	GGTACC	1 cut(s)	2467
AspA2I	CCTAGG	1 cut(s)	4295
AspLEI	GCGC	4 cut(s)	2320, 2356, 3113, 4247
AsuHPI	GGTGA	7 cut(s)	763, 1646, 1991, 2353, 2582, 2954, 3761
AvaI	CYCGRG	1 cut(s)	4906
AvaII	GGWCC	7 cut(s)	1349, 2107, 3580, 3811, 4999, 5149, 5216
AvrII	CCTAGG	1 cut(s)	4295
AxyI	CCTNAGG	1 cut(s)	849
BaeGI	GKGCMC	1 cut(s)	1485
BalI	TGGCCA	1 cut(s)	509
BamHI	GGATCC	1 cut(s)	604
BanI	GGYRCC	2 cut(s)	1482, 2467
BanII	GRGCYC	3 cut(s)	123, 777, 3020
BarI	GAAGNNNNNNTAC	4 cut(s)	3114, 3146, 3260, 3292
BauI	CACGAG	3 cut(s)	1476, 4590, 4614
BbrPI	CACGTG	1 cut(s)	2996
BbsI	GAAGAC	5 cut(s)	437, 1523, 3244, 4863, 5376
Bbv12I	GWGCWC	4 cut(s)	123, 1259, 3020, 4407
BceAI	ACGGC	1 cut(s)	1736
BcgI	CGANNNNNNTGC	8 cut(s)	479, 513, 522, 556, 1112, 1146, 4259, 4293
BciT130I	CCWGG	5 cut(s)	602, 1892, 2099, 3330, 5166
BciVI	GTATCC	3 cut(s)	1693, 2025, 2998
BclI	TGATCA	1 cut(s)	3871
BfmI	CTRYAG	3 cut(s)	1165, 3056, 5157
BfoI	RGCGCY	1 cut(s)	4248
BfrI	CTTAAG	3 cut(s)	2843, 3584, 5132
BfuAI	ACCTGC	2 cut(s)	1564, 4006
BfuI	GTATCC	3 cut(s)	1693, 2025, 2998
BglI	GCCNNNNNGGC	1 cut(s)	506
BglII	AGATCT	4 cut(s)	988, 1161, 1309, 4138
BlnI	CCTAGG	1 cut(s)	4295
BlpI	GCTNAGC	2 cut(s)	2878, 4401
BmcAI	AGTACT	1 cut(s)	1865
Bme1390I	CCNGG	5 cut(s)	602, 1892, 2099, 3330, 5166
Bme18I	GGWCC	7 cut(s)	1349, 2107, 3580, 3811, 4999, 5149, 5216
BmeT110I	CYCGRG	1 cut(s)	4906
BmrFI	CCNGG	5 cut(s)	602, 1892, 2099, 3330, 5166
BmrI	ACTGGG	6 cut(s)	951, 3251, 3506, 3537, 3768, 4227
BmuI	ACTGGG	6 cut(s)	951, 3251, 3506, 3537, 3768, 4227
BoxI	GACNNNNGTC	1 cut(s)	2391
BpiI	GAAGAC	5 cut(s)	437, 1523, 3244, 4863, 5376
BpmI	CTGGAG	4 cut(s)	38, 2081, 3464, 4172
Bpu1102I	GCTNAGC	2 cut(s)	2878, 4401
BpuEI	CTTGAG	5 cut(s)	240, 1898, 3560, 4776, 4778
BsaAI	YACGTR	3 cut(s)	31, 2416, 2996
BsaBI	GATNNNNATC	1 cut(s)	1101
BsaI	GGTCTC	2 cut(s)	3254, 4548
BsaJI	CCNNGG	9 cut(s)	523, 1422, 2074, 2166, 3329, 3745, 4295, 4626, 4907
BsaWI	WCCGGW	2 cut(s)	2939, 4100
BsaXI	ACNNNNNCTCC	4 cut(s)	11, 41, 4587, 4617
Bse118I	RCCGGY	1 cut(s)	2939
Bse21I	CCTNAGG	1 cut(s)	849
Bse3DI	GCAATG	1 cut(s)	1215
Bse8I	GATNNNNATC	1 cut(s)	1101
BseBI	CCWGG	5 cut(s)	602, 1892, 2099, 3330, 5166
BseDI	CCNNGG	9 cut(s)	523, 1422, 2074, 2166, 3329, 3745, 4295, 4626, 4907
BseJI	GATNNNNATC	1 cut(s)	1101
BseMI	GCAATG	1 cut(s)	1215
BseRI	GAGGAG	4 cut(s)	53, 377, 380, 5395
BseSI	GKGCMC	1 cut(s)	1485
BseYI	CCCAGC	1 cut(s)	86
Bsh1236I	CGCG	3 cut(s)	1437, 2318, 3113
Bsh1285I	CGRYCG	2 cut(s)	4433, 4576
BshFI	GGCC	9 cut(s)	404, 509, 908, 1068, 1421, 2073, 2584, 4460, 4847
BshNI	GGYRCC	2 cut(s)	1482, 2467
BshTI	ACCGGT	1 cut(s)	2939
BsiEI	CGRYCG	2 cut(s)	4433, 4576
BsiHKAI	GWGCWC	4 cut(s)	123, 1259, 3020, 4407
BsiHKCI	CYCGRG	1 cut(s)	4906
BsiSI	CCGG	2 cut(s)	2940, 4101
BsiWI	CGTACG	1 cut(s)	5226
BslFI	GGGAC	5 cut(s)	317, 2780, 5012, 5135, 5229
BsmFI	GGGAC	5 cut(s)	317, 2780, 5012, 5135, 5229
BsmI	GAATGC	2 cut(s)	2350, 4888
BsnI	GGCC	9 cut(s)	404, 509, 908, 1068, 1421, 2073, 2584, 4460, 4847
Bso31I	GGTCTC	2 cut(s)	3254, 4548
BsoBI	CYCGRG	1 cut(s)	4906
Bsp1286I	GDGCHC	6 cut(s)	123, 777, 1259, 1485, 3020, 4407
Bsp1407I	TGTACA	1 cut(s)	2125
Bsp1720I	GCTNAGC	2 cut(s)	2878, 4401
Bsp19I	CCATGG	1 cut(s)	2166
Bsp68I	TCGCGA	1 cut(s)	1437
BspANI	GGCC	9 cut(s)	404, 509, 908, 1068, 1421, 2073, 2584, 4460, 4847
BspFNI	CGCG	3 cut(s)	1437, 2318, 3113
BspHI	TCATGA	1 cut(s)	1576
BspMAI	CTGCAG	2 cut(s)	3060, 5161
BspMI	ACCTGC	2 cut(s)	1564, 4006
BspQI	GCTCTTC	3 cut(s)	2555, 5240, 5277
BspT107I	GGYRCC	2 cut(s)	1482, 2467
BspTI	CTTAAG	3 cut(s)	2843, 3584, 5132
BspTNI	GGTCTC	2 cut(s)	3254, 4548
BsrBI	CCGCTC	2 cut(s)	5242, 5392
BsrDI	GCAATG	1 cut(s)	1215
BsrFI	RCCGGY	1 cut(s)	2939
BsrGI	TGTACA	1 cut(s)	2125
BssAI	RCCGGY	1 cut(s)	2939
BssECI	CCNNGG	9 cut(s)	523, 1422, 2074, 2166, 3329, 3745, 4295, 4626, 4907
BssNAI	GTATAC	1 cut(s)	3857
BssSI	CACGAG	3 cut(s)	1476, 4590, 4614
BssT1I	CCWWGG	6 cut(s)	1422, 2074, 2166, 3745, 4295, 4626
Bst1107I	GTATAC	1 cut(s)	3857
Bst2BI	CACGAG	3 cut(s)	1476, 4590, 4614
Bst2UI	CCWGG	5 cut(s)	602, 1892, 2099, 3330, 5166
BstAFI	CTTAAG	3 cut(s)	2843, 3584, 5132
BstAUI	TGTACA	1 cut(s)	2125
BstBAI	YACGTR	3 cut(s)	31, 2416, 2996
BstC8I	GCNNGC	5 cut(s)	1255, 1725, 3365, 4160, 4876
BstDSI	CCRYGG	1 cut(s)	2166
BstEII	GGTNACC	1 cut(s)	1997
BstFNI	CGCG	3 cut(s)	1437, 2318, 3113
BstH2I	RGCGCY	1 cut(s)	4248
BstHHI	GCGC	4 cut(s)	2320, 2356, 3113, 4247
BstMCI	CGRYCG	2 cut(s)	4433, 4576
BstNI	CCWGG	5 cut(s)	602, 1892, 2099, 3330, 5166
BstNSI	RCATGY	4 cut(s)	1530, 2969, 3328, 3710
BstPAI	GACNNNNGTC	1 cut(s)	2391
BstPI	GGTNACC	1 cut(s)	1997
BstSCI	CCNGG	5 cut(s)	600, 1890, 2097, 3328, 5164
BstSFI	CTRYAG	3 cut(s)	1165, 3056, 5157
BstSLI	GKGCMC	1 cut(s)	1485
BstUI	CGCG	3 cut(s)	1437, 2318, 3113
BstV2I	GAAGAC	5 cut(s)	437, 1523, 3244, 4863, 5376
BstX2I	RGATCY	9 cut(s)	527, 604, 949, 988, 1161, 1309, 1426, 4138, 4322
BstXI	CCANNNNNNTGG	1 cut(s)	5326
BstYI	RGATCY	9 cut(s)	527, 604, 949, 988, 1161, 1309, 1426, 4138, 4322
BstZ17I	GTATAC	1 cut(s)	3857
Bsu36I	CCTNAGG	1 cut(s)	849
BsuI	GTATCC	3 cut(s)	1693, 2025, 2998
BsuRI	GGCC	9 cut(s)	404, 509, 908, 1068, 1421, 2073, 2584, 4460, 4847
BtgI	CCRYGG	1 cut(s)	2166
BtgZI	GCGATG	2 cut(s)	1452, 3128
BtsI	GCAGTG	2 cut(s)	177, 1275
BtuMI	TCGCGA	1 cut(s)	1437
BveI	ACCTGC	2 cut(s)	1564, 4006
Cac8I	GCNNGC	5 cut(s)	1255, 1725, 3365, 4160, 4876
CaiI	CAGNNNCTG	7 cut(s)	1063, 2104, 2633, 3101, 3442, 4487, 4958
CciI	TCATGA	1 cut(s)	1576
CfoI	GCGC	4 cut(s)	2320, 2356, 3113, 4247
Cfr10I	RCCGGY	1 cut(s)	2939
CseI	GACGC	4 cut(s)	1115, 4038, 4727, 4941
CspAI	ACCGGT	1 cut(s)	2939
CspCI	CAANNNNNGTGG	2 cut(s)	1668, 1703
DraI	TTTAAA	2 cut(s)	724, 4473
DrdI	GACNNNNNNGTC	1 cut(s)	3587
DseDI	GACNNNNNNGTC	1 cut(s)	3587
EaeI	YGGCCR	2 cut(s)	507, 4845
EciI	GGCGGA	1 cut(s)	3675
Ecl136II	GAGCTC	2 cut(s)	121, 3018
Eco130I	CCWWGG	6 cut(s)	1422, 2074, 2166, 3745, 4295, 4626
Eco24I	GRGCYC	3 cut(s)	123, 777, 3020
Eco31I	GGTCTC	2 cut(s)	3254, 4548
Eco32I	GATATC	2 cut(s)	1888, 2823
Eco47I	GGWCC	7 cut(s)	1349, 2107, 3580, 3811, 4999, 5149, 5216
Eco53kI	GAGCTC	2 cut(s)	121, 3018
Eco57I	CTGAAG	5 cut(s)	1760, 2169, 4035, 4269, 4959
Eco72I	CACGTG	1 cut(s)	2996
Eco81I	CCTNAGG	1 cut(s)	849
Eco88I	CYCGRG	1 cut(s)	4906
Eco91I	GGTNACC	1 cut(s)	1997
EcoICRI	GAGCTC	2 cut(s)	121, 3018
EcoO109I	RGGNCCY	2 cut(s)	3580, 5149
EcoO65I	GGTNACC	1 cut(s)	1997
EcoRI	GAATTC	2 cut(s)	1710, 2400
EcoRII	CCWGG	5 cut(s)	600, 1890, 2097, 3328, 5164
EcoRV	GATATC	2 cut(s)	1888, 2823
EcoT14I	CCWWGG	6 cut(s)	1422, 2074, 2166, 3745, 4295, 4626
EcoT22I	ATGCAT	1 cut(s)	553
EcoT38I	GRGCYC	3 cut(s)	123, 777, 3020
ErhI	CCWWGG	6 cut(s)	1422, 2074, 2166, 3745, 4295, 4626
FaqI	GGGAC	5 cut(s)	317, 2780, 5012, 5135, 5229
FauNDI	CATATG	2 cut(s)	1465, 1609
FbaI	TGATCA	1 cut(s)	3871
FblI	GTMKAC	3 cut(s)	2254, 2560, 3856
FriOI	GRGCYC	3 cut(s)	123, 777, 3020
GlaI	GCGC	4 cut(s)	2319, 2355, 3112, 4246
GsaI	CCCAGC	1 cut(s)	90
GsuI	CTGGAG	4 cut(s)	38, 2081, 3464, 4172
HaeII	RGCGCY	1 cut(s)	4248
HaeIII	GGCC	9 cut(s)	404, 509, 908, 1068, 1421, 2073, 2584, 4460, 4847
HapII	CCGG	2 cut(s)	2940, 4101
HgaI	GACGC	4 cut(s)	1115, 4038, 4727, 4941
HhaI	GCGC	4 cut(s)	2320, 2356, 3113, 4247
Hin6I	GCGC	4 cut(s)	2318, 2354, 3111, 4245
HinP1I	GCGC	4 cut(s)	2318, 2354, 3111, 4245
HincII	GTYRAC	4 cut(s)	2561, 3166, 3489, 3778
HindII	GTYRAC	4 cut(s)	2561, 3166, 3489, 3778
HindIII	AAGCTT	6 cut(s)	782, 2727, 4029, 4251, 5129, 5313
HpaI	GTTAAC	2 cut(s)	3489, 3778
HpaII	CCGG	2 cut(s)	2940, 4101
HphI	GGTGA	7 cut(s)	763, 1646, 1991, 2353, 2582, 2954, 3761
Hpy99I	CGWCG	5 cut(s)	1952, 2565, 4389, 4935, 5337
HspAI	GCGC	4 cut(s)	2318, 2354, 3111, 4245
KpnI	GGTACC	1 cut(s)	2471
Ksp22I	TGATCA	1 cut(s)	3871
KspAI	GTTAAC	2 cut(s)	3489, 3778
LguI	GCTCTTC	3 cut(s)	2555, 5240, 5277
LmnI	GCTCC	4 cut(s)	1846, 3023, 3688, 5140
MbiI	CCGCTC	2 cut(s)	5242, 5392
MfeI	CAATTG	2 cut(s)	1373, 1803
MflI	RGATCY	9 cut(s)	527, 604, 949, 988, 1161, 1309, 1426, 4138, 4322
MhlI	GDGCHC	6 cut(s)	123, 777, 1259, 1485, 3020, 4407
MlsI	TGGCCA	1 cut(s)	509
MluNI	TGGCCA	1 cut(s)	509
MlyI	GAGTC	9 cut(s)	238, 1054, 1606, 2639, 2822, 2930, 3455, 3800, 4578
MmeI	TCCRAC	5 cut(s)	156, 1327, 1677, 2139, 3214
Mox20I	TGGCCA	1 cut(s)	509
Mph1103I	ATGCAT	1 cut(s)	553
MroXI	GAANNNNTTC	3 cut(s)	1326, 2913, 2989
MscI	TGGCCA	1 cut(s)	509
MslI	CAYNNNNRTG	5 cut(s)	1790, 3174, 4316, 4419, 4773
Msp20I	TGGCCA	1 cut(s)	509
MspA1I	CMGCKG	1 cut(s)	4130
MspCI	CTTAAG	3 cut(s)	2843, 3584, 5132
MspI	CCGG	2 cut(s)	2940, 4101
MspR9I	CCNGG	5 cut(s)	602, 1892, 2099, 3330, 5166
MunI	CAATTG	2 cut(s)	1373, 1803
Mva1269I	GAATGC	2 cut(s)	2350, 4888
MvaI	CCWGG	5 cut(s)	602, 1892, 2099, 3330, 5166
MvnI	CGCG	3 cut(s)	1437, 2318, 3113
NcoI	CCATGG	1 cut(s)	2166
NdeI	CATATG	2 cut(s)	1465, 1609
NmeAIII	GCCGAG	3 cut(s)	548, 2440, 4045
NmuCI	GTSAC	8 cut(s)	31, 241, 979, 1997, 2371, 3370, 4309, 4436
NruI	TCGCGA	1 cut(s)	1437
NsiI	ATGCAT	1 cut(s)	553
NspI	RCATGY	4 cut(s)	1530, 2969, 3328, 3710
OliI	CACNNNNGTG	1 cut(s)	4316
PagI	TCATGA	1 cut(s)	1576
PciI	ACATGT	2 cut(s)	1526, 2965
PciSI	GCTCTTC	3 cut(s)	2555, 5240, 5277
PcsI	WCGNNNNNNNCGW	3 cut(s)	1219, 1228, 5201
PctI	GAATGC	2 cut(s)	2350, 4888
PdmI	GAANNNNTTC	3 cut(s)	1326, 2913, 2989
Pfl23II	CGTACG	1 cut(s)	5226
PflMI	CCANNNNNTGG	5 cut(s)	1571, 2104, 2116, 2416, 3442
PfoI	TCCNGGA	2 cut(s)	1890, 2097
PinAI	ACCGGT	1 cut(s)	2939
Ple19I	CGATCG	2 cut(s)	4433, 4576
PleI	GAGTC	9 cut(s)	238, 1054, 1605, 2638, 2821, 2929, 3454, 3799, 4577
PmaCI	CACGTG	1 cut(s)	2996
PmlI	CACGTG	1 cut(s)	2996
PpsI	GAGTC	9 cut(s)	238, 1054, 1605, 2638, 2821, 2929, 3454, 3799, 4577
Ppu21I	YACGTR	3 cut(s)	31, 2416, 2996
PpuMI	RGGWCCY	2 cut(s)	3580, 5149
PscI	ACATGT	2 cut(s)	1526, 2965
PshAI	GACNNNNGTC	1 cut(s)	2391
PshBI	ATTAAT	4 cut(s)	584, 927, 1956, 3042
PsiI	TTATAA	1 cut(s)	876
Psp124BI	GAGCTC	2 cut(s)	123, 3020
Psp1406I	AACGTT	1 cut(s)	283
Psp5II	RGGWCCY	2 cut(s)	3580, 5149
Psp6I	CCWGG	5 cut(s)	600, 1890, 2097, 3328, 5164
PspCI	CACGTG	1 cut(s)	2996
PspEI	GGTNACC	1 cut(s)	1997
PspFI	CCCAGC	1 cut(s)	86
PspGI	CCWGG	5 cut(s)	600, 1890, 2097, 3328, 5164
PspLI	CGTACG	1 cut(s)	5226
PspPPI	RGGWCCY	2 cut(s)	3580, 5149
PstI	CTGCAG	2 cut(s)	3060, 5161
PstNI	CAGNNNCTG	7 cut(s)	1063, 2104, 2633, 3101, 3442, 4487, 4958
PsuI	RGATCY	9 cut(s)	527, 604, 949, 988, 1161, 1309, 1426, 4138, 4322
PvuI	CGATCG	2 cut(s)	4433, 4576
PvuII	CAGCTG	1 cut(s)	4130
RruI	TCGCGA	1 cut(s)	1437
RseI	CAYNNNNRTG	5 cut(s)	1790, 3174, 4316, 4419, 4773
SacI	GAGCTC	2 cut(s)	123, 3020
SalI	GTCGAC	1 cut(s)	2559
SapI	GCTCTTC	3 cut(s)	2555, 5240, 5277
ScaI	AGTACT	1 cut(s)	1865
SchI	GAGTC	9 cut(s)	238, 1054, 1606, 2639, 2822, 2930, 3455, 3800, 4578
ScrFI	CCNGG	5 cut(s)	602, 1892, 2099, 3330, 5166
SduI	GDGCHC	6 cut(s)	123, 777, 1259, 1485, 3020, 4407
SfcI	CTRYAG	3 cut(s)	1165, 3056, 5157
SinI	GGWCC	7 cut(s)	1349, 2107, 3580, 3811, 4999, 5149, 5216
SmiI	ATTTAAAT	1 cut(s)	724
SmiMI	CAYNNNNRTG	5 cut(s)	1790, 3174, 4316, 4419, 4773
SmlI	CTYRAG	8 cut(s)	255, 1877, 2843, 3539, 3584, 4757, 4791, 5132
SmoI	CTYRAG	8 cut(s)	255, 1877, 2843, 3539, 3584, 4757, 4791, 5132
SspI	AATATT	1 cut(s)	4010
SstI	GAGCTC	2 cut(s)	123, 3020
StyD4I	CCNGG	5 cut(s)	600, 1890, 2097, 3328, 5164
StyI	CCWWGG	6 cut(s)	1422, 2074, 2166, 3745, 4295, 4626
SwaI	ATTTAAAT	1 cut(s)	724
TaqII	GACCGA	3 cut(s)	4455, 5016, 5031
TatI	WGTACW	5 cut(s)	1863, 2125, 3105, 3218, 3691
TauI	GCSGC	1 cut(s)	4850
TseFI	GTSAC	8 cut(s)	31, 241, 979, 1997, 2371, 3370, 4309, 4436
Tsp45I	GTSAC	8 cut(s)	31, 241, 979, 1997, 2371, 3370, 4309, 4436
TspGWI	ACGGA	6 cut(s)	50, 467, 2578, 2969, 3863, 5283
Van91I	CCANNNNNTGG	5 cut(s)	1571, 2104, 2116, 2416, 3442
Vha464I	CTTAAG	3 cut(s)	2843, 3584, 5132
VpaK11BI	GGWCC	7 cut(s)	1349, 2107, 3580, 3811, 4999, 5149, 5216
VspI	ATTAAT	4 cut(s)	584, 927, 1956, 3042
XapI	RAATTY	6 cut(s)	711, 725, 1710, 2400, 3653, 4945
XbaI	TCTAGA	3 cut(s)	1306, 3835, 4666
XceI	RCATGY	4 cut(s)	1530, 2969, 3328, 3710
XmaJI	CCTAGG	1 cut(s)	4295
XmiI	GTMKAC	3 cut(s)	2254, 2560, 3856
XmnI	GAANNNNTTC	3 cut(s)	1326, 2913, 2989
ZrmI	AGTACT	1 cut(s)	1865
Zsp2I	ATGCAT	1 cut(s)	553

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.