Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0003911

Physical Location & Seq

Genomic Coordinates Forward (+)

62837010 .. 62853233

Gene Structure 16224 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00383170.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5871 bp

ATGTCTCTCCCGCCCGTAGAGCGCTCACACATTAAGGAGGACCACCTCCGTGAATGGAAGAACGGAAACCCTAGCTTCAAGCTCCCGGAGCCCGTTCCGATGCTTCGGTTCCTCTACGAGCTCTGCTCCACCATGGTTCGTGGAGAATTGCCGTTTCAGAAGTGTAGAGCCGCTTTGGATTCGGTGGAGTTTTCCGAAAAGGTTTCCGAGCAAGAGCTGGCTTCAAGCTTGGCAGATATTGTCACTCAAATGTCTCAAGATGTGAGTTCTGTGAATGGATTTTCATGCTTGGAATTTGTTAAGTGCATTCGTTTCGGCAAGGGTTTTGATGTTACTCTTACCATGCCCGGAGAGCATCGAGCTCGTCTCACAAAACTGGCCAAATGGTTGGTGGAATCTTCTTTAGTTCCATTAAGGCTTTTTCAGGAGCGCTGTGAGGTTGATACTGATCCTTGTCCTAATAAACTTGACTGGCGGTTATCTGGTCATGAAGAATTTTTGTGGGAGGCTGAAATGATCAAGATAAAGGCTCAAGAACTGAAGAGTAAAGAGGTTAGAGTAAACACCCGTCTTCTTTATCAGCAAACAAAGTTTAATCTTCTTCGAGAAGAGAGCGAGGGATATGCCAAGCTGGTTACACTTCTTTGCCAAAATTCAGAAAATTCATCGCACAATGCAGGAGCAACAATAGGCATTATAAAGTCATTGATCGGACATTTTGATCTGGACCCAAATCATGTTTTTGATATCGTTTTGGAGTGTTTCGAACTTCTGCCAGACAACAATGTCTTTTTGGAGTTGATACCGATTTTTCCAAAGTCTCATGCTTCACAAATACTTGGTTTCAAATTTCAACATTACCAGCGTTTGGAAGTAAATGATCCTGTACCATTTGGACTTTATAAGCTTACGGCTTTGCTGGTGAAGGAGAATTTTATTGATCTTGATAGCATAGGCAATGCGAATGCCAGTTATTTGGGACCATTGGCACTAGCCACACCTTGGTTAGAAAAGCAGTACCCACTTTGGAAGCCGAAGACTTTGCCAATTGGGCGAGCTCTGAAACTTGCCCTTCTTTCGTTGATGGTGGAATCTTCATTGCAGACTAAGAGTCCCTTTGCATTATTGCGTGATGAGATTATCCTAATTGGCAGTTGCATTATTGCATGTGCTCATTTACTTCCGAAGGACGATGAGGCATTTGAGCACTACAGCTCTTTCTCCTCCAAGCAACTTGATGAGGCTAATAAAATTGGTAAAATTAATCTTGCTGCTACTGGGAAGGATCTAATGGAAGATGATAAACAAGGAGACGTGACAATAGATCTCTTTGCCTCTTTAGACATGGATTCAGAGGCAGTAGGAGAAAGATCTGCAGAGTTTGAAAATAATCAAACTTTGGGCTTGCTTACTGGTTTTCTTTCTGTAGATGACTGGTATCATGCACACGTACTATTTGACCGTCTGTCACCTCTAAATCCTGTTGAACATATCCAAATCTGTAATAGCTTGTTTAGATTGATTGAAAAGTCAATCTCTTCTGCATATGACATGGTTCGTCAAGCACGTCTCAATAACTTGGGGTCATCCTCAGGAACCAGTGTTGGTGTGATGACCACAGAAAACTCATCAGCTAGTGGTTCTTTTATAGAACTTAAAAAAGAACTTTTTCAGATGCTTGCTATTGTTGGGCCTTATCTTTATCGTGATACTTTGCTGCTACAGAAGGTTTGCAGAGTATTAAGAGGTTATTACTTGTCTGCTCCGGATCTTGGAAGGACTGGTGAAGTAGCTGTTTCTGCCAGTAATCCTCGTTTGCCTCTAAAGGAAGCTAGGTCCCCCCGTCAGGTTGCAATGTCAAGGGTAGAGGAAGCCTTCCGGACATGTCTACTTCCATCTTTACAGTTAGTACCTGCAAATCCAGCTGTTGGAATGGAGATTTGGGAAGTGATGAGTCTACTTCCTTATGAGGTGAGGTATCGTTTATATGGTGAATGGGAGAAAGAAGATGAACGGATTCCTATGGTTTTAGCTGCAAGGCAAACAGCCAAGTTGGACACTAGAAGGATTTTGAAGCGGCTTGCAAAAGAAAATTTAAAGCAGCAGGGTAGGATGGTTGCAAAATTAGCTCATGCAAATCCAATGACTGTACTTCGAACAATTGTTCATCAGATCGAGGCGTACAAAGATATGATTGCTCCTGTTGTAGATGCATTTAAGTATTTGACTCAGAGTTTTCTTACTTTAAAGAATATCTTGCTTGAAGTTTTTCAGAAGATTCTTTGTGGAGTAGAATCGCTGGAGTATGATGTCCTGGAATATGTTGTGATTGAACGTCTGGCGCAAGGAGGGCGTGATAAGCTAAAAGAGGATGGGCTTAACTTGTCAGACTGGCTTCAATCTTTGGCTTCATTTTGGGGTCACTTGCTAGAGGAGATTTTGATTGACATTCTCATTCTATGTGATGGAGCTTTCAGTAACTTCCTAGTTGTCTCAGGAATTGTTAGGTGTAAAAAATATCCATCTATGGAATTGAGAGGCCTTTTCCAGTATCTTGTAAATCAGTTGAAAAAGGGCCAAGGAATTGAGCTTGTTGTTCTTCAGGAGCTTATTCAGCAAATGGCAAATGTCCATCACACAGAGGACCTTACTGAAGATCAGCTGGATGCCATGGCAGGCGGTGAGACTCTCCGTCATCACGCCACTGGTTTTGGTGTTACTCGACATAACAAGCAACTACTCAAGTCCACAAACAGGCTTAGGGACTCGCTGCTTCCAAAGGATGAAACCAAGCTGGCAATCCCTCTTTTGTTACATCTTGCTCAGCATCGTTCTTTGGTCATAATAGATGCAGATGCGCCTTATATTAAGATGGTTAGCGAGCAGTTTGATAGATGTCACGGAGCACTCCTTCAGTATGTGGAATTTCTTTGCAGTGCCATGCCATCGGCATCAGCTTATGCTCAGCTAATTCCTTCACTTGATGATCTTGTCCATAAATACCACCTTGATCCTGAGGTTGCTTTCTTAATTTATCGCCCAGTAATGAGGCTCTTCAAGAGCCCTGCGAGTTCTGATGTCTTCTGGCCTCTAGACAATAATGATGCTCAAAGTATCACATCTGCAAATTCAGAGTCTGAAGCTGTACAGAACTCTGGCAACGTGGTTCTGGATCTCGGGTCTACATGGAACCCTATAACGTGGTTGGATCTTCTTCATACTGCTAAAGCTATGTTGCCTGCAAGAGCCTGGAATAGTTTGTCCCCTGATCTTTATGCTACATTTTGGGGGCTCACACTTTATGATCTTTATGTCCCTAGAAATTGTTATGAATCTGAAATTGCGAAACAGCAGGCTTCTCTTAAAGTTCTGGAGGAGCAGCCTGATAATTCAATTTCTGCAATAACCAAAAGGAAGAAAGAGAAGGAAAGAATTCAAGATACCATAGATCGCCTGACTAGTGAACTCCGTAAACATGAGGAACATGTTGCATCTGTTCGTAAACGCTTATCGCATGAGAAGGACAAATGGTTGAGTTCTTGTCTTGATACTTTAAAGATCAACATGGAATTCCTTCAGCGTTGTATATTTCCTCGCTGTACTTTCAGCATGCCAGATGCTGTCTATTGTGCCATGTTTGTGCACACTCTTCATTCCCTGGGGACACCATTTTTTAACACAGTCAACCACATGGATGTTCTTATATGTAGAACCTTACAGCCTATGATATGCTGCTGCACTGAATCTGAAGTTGGTAGACTGGGGAAGTTTCTCTGTGAGACCTTGAAAATTGCTTACTATTGGAAGAGTGATGAATCTATTTATGAGCGCGAGTGCGGAAACATGCCAGGATTTGCTGTCTACTATAGGTTTCCAGATAGCCAGCGGGTCCGATATGGCCAGTTTGTCAAGGTGCACTGGAAATGGAGTCAAAGGATCACGAGATTATTGGGTCAGTGTCTGGAATCTCCGGAGTACATGGAAATTCGAAACGCTCTCATAATCTTATCAAGAATTTCTAGTGTTTTCCCGGTTACTAAAAAGAGTGGGATGAACCTTGAAAGACGGGTGTCCAAGATCAAAGGTGATGGGAGAGAAGACCTCAAGGTGTTGGCAACAAGTGTTGGTGCTTCTTTGGCTGCTAGAAAGCCTTCATTGGTTTCGGATGAAGAATTTTGTATGGGATACGTTGAATTAAAGTCGACAGCATCTTCAAAGCCTTCAGCCAGTAATTCAGGAGCTATACAGAGTGGTCCTGCCGTTAATAATTCTCAAACCGAACCTGTTGGCGGAAGAGCAGGAAACCTTGTCTCCCAACACTCAGAATTAATAAACTCAGCCAGAGACCACGTGTCAAAAACCAAACCTGCAGATGGGAGGTTGGAAAGAGCAGAAAGTATCTCAACTGCAAAATCTGACCAAGGGCATCTAAAACTGAAAGGTGCCTCATTGGTGAATGGCTCAGATGCTCAGGCATCCGTGCCATCAGCTACTTTACAATCTGGAACATCAAGGCCTATTGAAAATCAAGTACAAGTGAACGAAATCTCAAATCGAACGCCAGATGAAAATATGGGAAAACTTGCCGCAAAGAACACTTCAGAATCTGAGTTGAGGGCCCAAGCAAAACGGTCTGTTCCTGCTGGGGCTAAGCCATTGAAACAAGATCTTGCGAAGGACGAAAGTAGATCTGGAAAAGCAGCTGGAGCTACTAATGCTTCTTCCATCACCGCAAATGGTAGCACGGTATCATCCTCAGGCAAAGGTTCAGCTTCGTTAGGTATTGAATCAAAAGTGGAGGCTGGATCTGCAAAGTTTTCCAATACAAGGATCCCTTCTTCAAAGGAAGAGGGTGCTGAGGTATCTGATGCAGCAAGGCCCCCTTCTTCTCGATTTGTTCATTCTCCTAGACATGATAGTTCTGCTACACTCTCAAAGTCCAGTGATAAACTTCAAAAAAGAACCAGCCCTGCAGAAGAAACAGATAGACAAAGAAGCGATGATAGAAGTGTATATAAGGCCACTGAAAAGGCTTCAGATCGGTCTAAAGATAAAGGAAGTGAAAGACATGAGAAGGATCACAGGGAAAGATTGGACCGTCCTGATAAGTCACGCGGAGATGATTTAGTTGAGAGATCCAGAGATAGGTCAATGGAGAGACATGGAAGGGATCATTCAGCTGAGAAATTGCAAGAGAGGGGTACAGATAGGACTTTTGATAGACTGCCTGACAAATCTAAGGATGAGAAAGGTAAAGGGCGTTACGGTGATATTTCTGCTGAAAAATCTCATGTTGATGATCGATATCACGGGCAAAGCTTACCTCCACCACCGCCCTTACCTCCTCACATTGTTCCTCAATCTGTTAGTTCTGGAAGAAGAGACGAAGATTCAGATAGAAGGACTGCAAATACAAGGCATACTCAAAGGCTTTCCCCGAGACATGATGAGAAGGAACGTAGACGATCTGAAGAAAATTCCTCAATTTCTCAAGATGATTCAAAGCGTAGAAGAGAAGATGATTTTCGAGAGAGAAAGCGTGATGATCGTGAGGGGACCTCACTAAAGGTGGAAGAGAGGGACAGGGAGAGGGAAAGGGAGCGGGAGAGGGAGAAAGAAAGGGAGAAAGCCAACCTCTTGAAGGAGGATTCAGACTCAATTGCAGCTTCTAAGAGGCGGAAACTTAAACGAGAACATCTGACTTCTGGGGAAGCTGGTTTCATATTATCCACTAGTGCTGAATGGAATCCTATTTTCACAACTGTGTTGAGATCTATCATGAGCATTGCTGTGCGTGCATATATGATGTACGACTGGGACGACGATAAAAGGCAACGAGGTGAACAGAAACGAAGGCATCGGAAGAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000346 GO:0000347 GO:0000445 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006396 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0008104 GO:0008150 GO:0008152 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0015031 GO:0015833 GO:0015931 GO:0016070 GO:0016441 GO:0016458 GO:0016604 GO:0016607 GO:0019222 GO:0031047 GO:0031050 GO:0031503 GO:0031974 GO:0031981 GO:0032991 GO:0033036 GO:0034613 GO:0034641 GO:0035194 GO:0035195 GO:0035196 GO:0040029 GO:0042221 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048519 GO:0050657 GO:0050658 GO:0050789 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070727 GO:0070887 GO:0070918 GO:0071166 GO:0071310 GO:0071359 GO:0071407 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1990428

KEGG Pathways

Metabolic & Signaling

ko03013 ko03040 map03013 map03040

Pfam Domains

Protein Families

THOC2_N Tho2 Thoc2

Protein Analysis

1956

Amino Acids

220.76

Weight (kDa)

7.55

Isoelectric Point (pI)

47.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
THOC2_N	PF16134	175 - 318	3.4e-31	THO complex subunit 2 N-terminus
THOC2_N	PF16134	555 - 704	3.3e-21	THO complex subunit 2 N-terminus
Thoc2	PF11732	706 - 744	5.5e-11	Transcription- and export-related complex subunit
Thoc2	PF11732	764 - 803	9.7e-11	Transcription- and export-related complex subunit
Tho2	PF11262	1084 - 1378	6.3e-96	Transcription factor/nuclear export subunit protein 2

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0003911)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G24706 AT1G24706 AT1G24706 AT1G24706 AT1G24706 AT1G24706 AT1G24706 AT1G24706 AT1G24706
fragaria_vesca	FvH4_4g33200 FvH4_4g33200 FvH4_4g33200
malus_domestica	MD09G1258800.v1.1
prunus_persica	Prupe.3G150800_v2.0.a1 Prupe.3G150800_v2.0.a1 Prupe.3G150800_v2.0.a1 Prupe.3G150800_v2.0.a1 Prupe.3G150800_v2.0.a1 Prupe.3G150800_v2.0.a1 Prupe.3G150800_v2.0.a1 Prupe.3G150800_v2.0.a1 Prupe.3G150800_v2.0.a1
pyrus_communis	pycom09g17360
rosa_chinensis	RchiOBHm_Chr4g0442311
rosa_laevigata	RLG00000006018
rosa_multiflora	Rmu_sc0000124.1_g000055
rosa_roxburghii	Rroxscaffold_5G00383170
rosa_rugosa	Rorug04G0340300 Rorug04G0340400
rosa_samantha	Rh4AG391900 Rh4BG405400 Rh4CG420600 Rh4DG398700
rosa_wichuraiana	Rw4G033850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	698, 903
AasI	GACNNNNNNGTC	1 cut(s)	785
Acc36I	ACCTGC	2 cut(s)	1921, 4375
AccB1I	GGYRCC	1 cut(s)	4442
AccB7I	CCANNNNNTGG	3 cut(s)	868, 1002, 1928
AccBSI	CCGCTC	1 cut(s)	5602
AccI	GTMKAC	7 cut(s)	1888, 1957, 3182, 3757, 3861, 4202, 5461
AccII	CGCG	2 cut(s)	3831, 5115
AccIII	TCCGGA	3 cut(s)	1765, 1878, 3970
AcoI	YGGCCR	2 cut(s)	378, 3898
AcvI	CACGTG	1 cut(s)	4351
AfeI	AGCGCT	2 cut(s)	23, 431
AflIII	ACRYGT	4 cut(s)	1447, 1883, 3484, 4350
AhdI	GACNNNNNGTC	4 cut(s)	1110, 1465, 2691, 4351
AhlI	ACTAGT	2 cut(s)	3458, 5732
AjiI	CACGTC	2 cut(s)	1315, 1568
AjnI	CCWGG	4 cut(s)	2313, 3248, 3657, 3847
AjuI	GAANNNNNNNTTGG	4 cut(s)	1924, 1956, 3735, 3767
AleI	CACNNNNGTG	2 cut(s)	48, 5762
Alw21I	GWGCWC	8 cut(s)	123, 364, 1060, 1174, 1209, 2908, 3645, 3918
Alw44I	GTGCAC	2 cut(s)	3641, 3914
AlwNI	CAGNNNCTG	4 cut(s)	3137, 3620, 3961, 4607
Ama87I	CYCGRG	2 cut(s)	3176, 5437
Aor13HI	TCCGGA	3 cut(s)	1765, 1878, 3970
Aor51HI	AGCGCT	2 cut(s)	23, 431
ApaI	GGGCCC	1 cut(s)	4621
ApaLI	GTGCAC	2 cut(s)	3641, 3914
ArsI	GACNNNNNNTTYG	2 cut(s)	3119, 3151
AseI	ATTAAT	2 cut(s)	1263, 4328
Asp700I	GAANNNNTTC	6 cut(s)	1668, 2016, 2268, 3432, 3573, 4150
AspLEI	GCGC	5 cut(s)	24, 432, 2344, 2860, 3831
AsuC2I	CCSGG	3 cut(s)	86, 348, 4031
AsuII	TTCGAA	3 cut(s)	765, 2155, 3988
AvaI	CYCGRG	2 cut(s)	3176, 5437
AvaII	GGWCC	9 cut(s)	40, 727, 980, 1836, 2644, 3889, 4253, 5095, 5556
AxyI	CCTNAGG	3 cut(s)	1591, 3015, 4756
BaeGI	GKGCMC	3 cut(s)	3645, 3918, 4621
BalI	TGGCCA	2 cut(s)	380, 3900
BamHI	GGATCC	1 cut(s)	4830
BanI	GGYRCC	1 cut(s)	4442
BanII	GRGCYC	7 cut(s)	93, 123, 364, 1060, 3065, 3294, 4621
BauI	CACGAG	1 cut(s)	3940
BbrPI	CACGTG	1 cut(s)	4351
BbsI	GAAGAC	4 cut(s)	563, 1043, 3073, 4104
Bbv12I	GWGCWC	8 cut(s)	123, 364, 1060, 1174, 1209, 2908, 3645, 3918
BbvCI	CCTCAGC	1 cut(s)	4857
BceAI	ACGGC	3 cut(s)	136, 927, 4244
BcgI	CGANNNNNNTGC	8 cut(s)	295, 329, 344, 378, 605, 639, 1024, 1058
BciT130I	CCWGG	4 cut(s)	2315, 3250, 3659, 3849
BciVI	GTATCC	1 cut(s)	4178
BclI	TGATCA	1 cut(s)	516
BcnI	CCSGG	3 cut(s)	86, 348, 4031
BcuI	ACTAGT	2 cut(s)	3458, 5732
BfmI	CTRYAG	7 cut(s)	1210, 1374, 1425, 1721, 3865, 4368, 4971
BfoI	RGCGCY	2 cut(s)	25, 433
BfuAI	ACCTGC	2 cut(s)	1921, 4375
BfuI	GTATCC	1 cut(s)	4178
BglI	GCCNNNNNGGC	1 cut(s)	1051
BglII	AGATCT	5 cut(s)	1324, 1370, 4666, 4688, 5771
BlpI	GCTNAGC	3 cut(s)	2823, 2964, 4650
Bme1390I	CCNGG	7 cut(s)	86, 348, 2315, 3250, 3659, 3849, 4031
Bme18I	GGWCC	9 cut(s)	40, 727, 980, 1836, 2644, 3889, 4253, 5095, 5556
BmeRI	GACNNNNNGTC	4 cut(s)	1110, 1465, 2691, 4351
BmeT110I	CYCGRG	2 cut(s)	3176, 5437
BmgBI	CACGTC	2 cut(s)	1315, 1568
BmrFI	CCNGG	7 cut(s)	86, 348, 2315, 3250, 3659, 3849, 4031
BmrI	ACTGGG	4 cut(s)	1287, 3035, 3770, 5824
BmuI	ACTGGG	4 cut(s)	1287, 3035, 3770, 5824
BoxI	GACNNNNGTC	1 cut(s)	4069
BpiI	GAAGAC	4 cut(s)	563, 1043, 3073, 4104
BplI	GAGNNNNNCTC	8 cut(s)	110, 142, 351, 383, 4086, 4118, 5543, 5575
BpmI	CTGGAG	3 cut(s)	2321, 3392, 4725
Bpu10I	CCTNAGC	3 cut(s)	2759, 4470, 4857
Bpu1102I	GCTNAGC	3 cut(s)	2823, 2964, 4650
Bpu14I	TTCGAA	3 cut(s)	765, 2155, 3988
BpuEI	CTTGAG	5 cut(s)	240, 516, 2726, 4088, 5475
BpuMI	CCSGG	3 cut(s)	86, 348, 4031
Bsa29I	ATCGAT	1 cut(s)	5302
BsaAI	YACGTR	2 cut(s)	1450, 4351
BsaBI	GATNNNNATC	2 cut(s)	447, 5304
BsaI	GGTCTC	2 cut(s)	3773, 4338
BsaJI	CCNNGG	7 cut(s)	132, 1001, 2578, 2670, 3657, 3658, 4420
BsaWI	WCCGGW	3 cut(s)	1765, 1878, 3970
BsaXI	ACNNNNNCTCC	2 cut(s)	4295, 4325
Bse21I	CCTNAGG	3 cut(s)	1591, 3015, 4756
Bse3DI	GCAATG	4 cut(s)	964, 1097, 1860, 5784
Bse8I	GATNNNNATC	2 cut(s)	447, 5304
BseAI	TCCGGA	3 cut(s)	1765, 1878, 3970
BseBI	CCWGG	4 cut(s)	2315, 3250, 3659, 3849
BseCI	ATCGAT	1 cut(s)	5302
BseDI	CCNNGG	7 cut(s)	132, 1001, 2578, 2670, 3657, 3658, 4420
BseJI	GATNNNNATC	2 cut(s)	447, 5304
BseMI	GCAATG	4 cut(s)	964, 1097, 1860, 5784
BseRI	GAGGAG	4 cut(s)	1213, 2447, 3389, 5334
BseSI	GKGCMC	3 cut(s)	3645, 3918, 4621
BseYI	CCCAGC	1 cut(s)	4643
BsgI	GTGCAG	1 cut(s)	3721
Bsh1236I	CGCG	2 cut(s)	3831, 5115
BshNI	GGYRCC	1 cut(s)	4442
BshVI	ATCGAT	1 cut(s)	5302
BsiHKAI	GWGCWC	8 cut(s)	123, 364, 1060, 1174, 1209, 2908, 3645, 3918
BsiHKCI	CYCGRG	2 cut(s)	3176, 5437
BsiSI	CCGG	6 cut(s)	86, 348, 1766, 1879, 3971, 4031
BsmBI	CGTCTC	4 cut(s)	371, 1305, 1574, 5376
BsmI	GAATGC	2 cut(s)	306, 970
Bso31I	GGTCTC	2 cut(s)	3773, 4338
BsoBI	CYCGRG	2 cut(s)	3176, 5437
Bsp119I	TTCGAA	3 cut(s)	765, 2155, 3988
Bsp120I	GGGCCC	1 cut(s)	4617
Bsp13I	TCCGGA	3 cut(s)	1765, 1878, 3970
Bsp1407I	TGTACA	1 cut(s)	3145
Bsp1720I	GCTNAGC	3 cut(s)	2823, 2964, 4650
Bsp19I	CCATGG	2 cut(s)	132, 2670
BspDI	ATCGAT	1 cut(s)	5302
BspEI	TCCGGA	3 cut(s)	1765, 1878, 3970
BspFNI	CGCG	2 cut(s)	3831, 5115
BspHI	TCATGA	2 cut(s)	487, 5778
BspMAI	CTGCAG	3 cut(s)	1378, 4372, 4975
BspMI	ACCTGC	2 cut(s)	1921, 4375
BspQI	GCTCTTC	2 cut(s)	3059, 4288
BspT104I	TTCGAA	3 cut(s)	765, 2155, 3988
BspT107I	GGYRCC	1 cut(s)	4442
BspTNI	GGTCTC	2 cut(s)	3773, 4338
BsrBI	CCGCTC	1 cut(s)	5602
BsrDI	GCAATG	4 cut(s)	964, 1097, 1860, 5784
BsrGI	TGTACA	1 cut(s)	3145
BssECI	CCNNGG	7 cut(s)	132, 1001, 2578, 2670, 3657, 3658, 4420
BssSI	CACGAG	1 cut(s)	3940
BssT1I	CCWWGG	5 cut(s)	132, 1001, 2578, 2670, 4420
Bst2BI	CACGAG	1 cut(s)	3940
Bst2UI	CCWGG	4 cut(s)	2315, 3250, 3659, 3849
Bst4CI	ACNGT	9 cut(s)	1463, 1905, 2149, 3682, 4632, 4747, 5099, 5267, 5764
BstAUI	TGTACA	1 cut(s)	3145
BstBAI	YACGTR	2 cut(s)	1450, 4351
BstBI	TTCGAA	3 cut(s)	765, 2155, 3988
BstDSI	CCRYGG	2 cut(s)	132, 2670
BstENI	CCTNNNNNAGG	1 cut(s)	5639
BstFNI	CGCG	2 cut(s)	3831, 5115
BstH2I	RGCGCY	2 cut(s)	25, 433
BstHHI	GCGC	5 cut(s)	24, 432, 2344, 2860, 3831
BstNI	CCWGG	4 cut(s)	2315, 3250, 3659, 3849
BstNSI	RCATGY	5 cut(s)	1170, 1887, 3488, 3613, 3847
BstPAI	GACNNNNGTC	1 cut(s)	4069
BstSCI	CCNGG	7 cut(s)	84, 346, 2313, 3248, 3657, 3847, 4029
BstSFI	CTRYAG	7 cut(s)	1210, 1374, 1425, 1721, 3865, 4368, 4971
BstSLI	GKGCMC	3 cut(s)	3645, 3918, 4621
BstUI	CGCG	2 cut(s)	3831, 5115
BstV2I	GAAGAC	4 cut(s)	563, 1043, 3073, 4104
BstXI	CCANNNNNNTGG	2 cut(s)	388, 976
Bsu15I	ATCGAT	1 cut(s)	5302
Bsu36I	CCTNAGG	3 cut(s)	1591, 3015, 4756
BsuI	GTATCC	1 cut(s)	4178
BsuTUI	ATCGAT	1 cut(s)	5302
BtgI	CCRYGG	2 cut(s)	132, 2670
BtgZI	GCGATG	2 cut(s)	651, 5013
BtrI	CACGTC	2 cut(s)	1315, 1568
BtsI	GCAGTG	1 cut(s)	2941
BtsIMutI	CAGTG	8 cut(s)	1606, 2703, 2941, 3738, 3916, 3962, 4948, 5022
BveI	ACCTGC	2 cut(s)	1921, 4375
CaiI	CAGNNNCTG	4 cut(s)	3137, 3620, 3961, 4607
CciI	TCATGA	2 cut(s)	487, 5778
CfoI	GCGC	5 cut(s)	24, 432, 2344, 2860, 3831
ClaI	ATCGAT	1 cut(s)	5302
CspCI	CAANNNNNGTGG	2 cut(s)	2692, 2727
DraI	TTTAAA	3 cut(s)	2097, 2247, 3555
DrdI	GACNNNNNNGTC	1 cut(s)	785
DriI	GACNNNNNGTC	4 cut(s)	1110, 1465, 2691, 4351
DseDI	GACNNNNNNGTC	1 cut(s)	785
EaeI	YGGCCR	2 cut(s)	378, 3898
Eam1105I	GACNNNNNGTC	4 cut(s)	1110, 1465, 2691, 4351
EciI	GGCGGA	2 cut(s)	4305, 5692
Ecl136II	GAGCTC	3 cut(s)	121, 362, 1058
Eco130I	CCWWGG	5 cut(s)	132, 1001, 2578, 2670, 4420
Eco147I	AGGCCT	2 cut(s)	2541, 4516
Eco24I	GRGCYC	7 cut(s)	93, 123, 364, 1060, 3065, 3294, 4621
Eco31I	GGTCTC	2 cut(s)	3773, 4338
Eco32I	GATATC	2 cut(s)	748, 5306
Eco47I	GGWCC	9 cut(s)	40, 727, 980, 1836, 2644, 3889, 4253, 5095, 5556
Eco47III	AGCGCT	2 cut(s)	23, 431
Eco53kI	GAGCTC	3 cut(s)	121, 362, 1058
Eco72I	CACGTG	1 cut(s)	4351
Eco81I	CCTNAGG	3 cut(s)	1591, 3015, 4756
Eco88I	CYCGRG	2 cut(s)	3176, 5437
EcoICRI	GAGCTC	3 cut(s)	121, 362, 1058
EcoNI	CCTNNNNNAGG	1 cut(s)	5639
EcoO109I	RGGNCCY	5 cut(s)	1836, 2644, 4617, 4878, 5556
EcoRI	GAATTC	2 cut(s)	3432, 3569
EcoRII	CCWGG	4 cut(s)	2313, 3248, 3657, 3847
EcoRV	GATATC	2 cut(s)	748, 5306
EcoT14I	CCWWGG	5 cut(s)	132, 1001, 2578, 2670, 4420
EcoT22I	ATGCAT	1 cut(s)	2215
EcoT38I	GRGCYC	7 cut(s)	93, 123, 364, 1060, 3065, 3294, 4621
ErhI	CCWWGG	5 cut(s)	132, 1001, 2578, 2670, 4420
Esp3I	CGTCTC	4 cut(s)	371, 1305, 1574, 5376
FalI	AAGNNNNNCTT	8 cut(s)	1100, 1132, 2240, 2272, 4819, 4851, 4867, 4899
FauI	CCCGC	3 cut(s)	18, 3879, 5595
FauNDI	CATATG	1 cut(s)	1546
FbaI	TGATCA	1 cut(s)	516
FblI	GTMKAC	7 cut(s)	1888, 1957, 3182, 3757, 3861, 4202, 5461
FriOI	GRGCYC	7 cut(s)	93, 123, 364, 1060, 3065, 3294, 4621
GlaI	GCGC	5 cut(s)	23, 431, 2343, 2859, 3830
GsaI	CCCAGC	1 cut(s)	4647
GsuI	CTGGAG	3 cut(s)	2321, 3392, 4725
HaeII	RGCGCY	2 cut(s)	25, 433
HapII	CCGG	6 cut(s)	86, 348, 1766, 1879, 3971, 4031
HhaI	GCGC	5 cut(s)	24, 432, 2344, 2860, 3831
Hin6I	GCGC	5 cut(s)	22, 430, 2342, 2858, 3829
HinP1I	GCGC	5 cut(s)	22, 430, 2342, 2858, 3829
HincII	GTYRAC	2 cut(s)	3685, 4203
HindII	GTYRAC	2 cut(s)	3685, 4203
HindIII	AAGCTT	3 cut(s)	226, 905, 5317
HpaII	CCGG	6 cut(s)	86, 348, 1766, 1879, 3971, 4031
Hpy99I	CGWCG	1 cut(s)	5825
HpyCH4III	ACNGT	9 cut(s)	1463, 1905, 2149, 3682, 4632, 4747, 5099, 5267, 5764
HpyCH4IV	ACGT	9 cut(s)	1314, 1449, 1567, 2335, 3162, 3200, 4188, 4350, 5458
HpySE526I	ACGT	9 cut(s)	1314, 1449, 1567, 2335, 3162, 3200, 4188, 4350, 5458
HspAI	GCGC	5 cut(s)	22, 430, 2342, 2858, 3829
Kpn2I	TCCGGA	3 cut(s)	1765, 1878, 3970
Ksp22I	TGATCA	1 cut(s)	516
LguI	GCTCTTC	2 cut(s)	3059, 4288
MaeII	ACGT	9 cut(s)	1314, 1449, 1567, 2335, 3162, 3200, 4188, 4350, 5458
MbiI	CCGCTC	1 cut(s)	5602
MfeI	CAATTG	3 cut(s)	1047, 2160, 5658
MlsI	TGGCCA	2 cut(s)	380, 3900
MluNI	TGGCCA	2 cut(s)	380, 3900
MlyI	GAGTC	8 cut(s)	1120, 1963, 2221, 2680, 2759, 3143, 3937, 5648
MmeI	TCCRAC	4 cut(s)	1909, 2034, 3186, 4362
Mox20I	TGGCCA	2 cut(s)	380, 3900
Mph1103I	ATGCAT	1 cut(s)	2215
MroI	TCCGGA	3 cut(s)	1765, 1878, 3970
MroXI	GAANNNNTTC	6 cut(s)	1668, 2016, 2268, 3432, 3573, 4150
MscI	TGGCCA	2 cut(s)	380, 3900
MslI	CAYNNNNRTG	7 cut(s)	48, 248, 3638, 3693, 5295, 5762, 5789
Msp20I	TGGCCA	2 cut(s)	380, 3900
MspA1I	CMGCKG	5 cut(s)	1925, 2662, 3886, 4703, 5180
MspI	CCGG	6 cut(s)	86, 348, 1766, 1879, 3971, 4031
MspR9I	CCNGG	7 cut(s)	86, 348, 2315, 3250, 3659, 3849, 4031
MunI	CAATTG	3 cut(s)	1047, 2160, 5658
Mva1269I	GAATGC	2 cut(s)	306, 970
MvaI	CCWGG	4 cut(s)	2315, 3250, 3659, 3849
MvnI	CGCG	2 cut(s)	3831, 5115
NciI	CCSGG	3 cut(s)	86, 348, 4031
NcoI	CCATGG	2 cut(s)	132, 2670
NdeI	CATATG	1 cut(s)	1546
NmuCI	GTSAC	6 cut(s)	241, 1315, 1467, 2420, 2897, 5109
NsiI	ATGCAT	1 cut(s)	2215
NspI	RCATGY	5 cut(s)	1170, 1887, 3488, 3613, 3847
NspV	TTCGAA	3 cut(s)	765, 2155, 3988
OliI	CACNNNNGTG	2 cut(s)	48, 5762
PaeI	GCATGC	1 cut(s)	3613
PagI	TCATGA	2 cut(s)	487, 5778
PasI	CCCWGGG	1 cut(s)	3658
PceI	AGGCCT	2 cut(s)	2541, 4516
PciI	ACATGT	2 cut(s)	1883, 3484
PciSI	GCTCTTC	2 cut(s)	3059, 4288
PcsI	WCGNNNNNNNCGW	2 cut(s)	1564, 5817
PctI	GAATGC	2 cut(s)	306, 970
PdmI	GAANNNNTTC	6 cut(s)	1668, 2016, 2268, 3432, 3573, 4150
PflMI	CCANNNNNTGG	3 cut(s)	868, 1002, 1928
PfoI	TCCNGGA	2 cut(s)	84, 2313
PleI	GAGTC	8 cut(s)	1119, 1962, 2221, 2680, 2759, 3142, 3936, 5648
PmaCI	CACGTG	1 cut(s)	4351
PmlI	CACGTG	1 cut(s)	4351
PpsI	GAGTC	8 cut(s)	1119, 1962, 2221, 2680, 2759, 3142, 3936, 5648
Ppu21I	YACGTR	2 cut(s)	1450, 4351
PpuMI	RGGWCCY	3 cut(s)	1836, 2644, 5556
PscI	ACATGT	2 cut(s)	1883, 3484
PshAI	GACNNNNGTC	1 cut(s)	4069
PshBI	ATTAAT	2 cut(s)	1263, 4328
PsiI	TTATAA	2 cut(s)	698, 903
Psp124BI	GAGCTC	3 cut(s)	123, 364, 1060
Psp5II	RGGWCCY	3 cut(s)	1836, 2644, 5556
Psp6I	CCWGG	4 cut(s)	2313, 3248, 3657, 3847
PspCI	CACGTG	1 cut(s)	4351
PspFI	CCCAGC	1 cut(s)	4643
PspGI	CCWGG	4 cut(s)	2313, 3248, 3657, 3847
PspOMI	GGGCCC	1 cut(s)	4617
PspPPI	RGGWCCY	3 cut(s)	1836, 2644, 5556
PstI	CTGCAG	3 cut(s)	1378, 4372, 4975
PstNI	CAGNNNCTG	4 cut(s)	3137, 3620, 3961, 4607
PvuII	CAGCTG	4 cut(s)	1925, 2662, 4703, 5180
RseI	CAYNNNNRTG	7 cut(s)	48, 248, 3638, 3693, 5295, 5762, 5789
SacI	GAGCTC	3 cut(s)	123, 364, 1060
SalI	GTCGAC	1 cut(s)	4201
SapI	GCTCTTC	2 cut(s)	3059, 4288
SchI	GAGTC	8 cut(s)	1120, 1963, 2221, 2680, 2759, 3143, 3937, 5648
ScrFI	CCNGG	7 cut(s)	86, 348, 2315, 3250, 3659, 3849, 4031
SfcI	CTRYAG	7 cut(s)	1210, 1374, 1425, 1721, 3865, 4368, 4971
SfuI	TTCGAA	3 cut(s)	765, 2155, 3988
SinI	GGWCC	9 cut(s)	40, 727, 980, 1836, 2644, 3889, 4253, 5095, 5556
SmiMI	CAYNNNNRTG	7 cut(s)	48, 248, 3638, 3693, 5295, 5762, 5789
SmlI	CTYRAG	5 cut(s)	255, 531, 2741, 4103, 5490
SmoI	CTYRAG	5 cut(s)	255, 531, 2741, 4103, 5490
SpeI	ACTAGT	2 cut(s)	3458, 5732
SphI	GCATGC	1 cut(s)	3613
SseBI	AGGCCT	2 cut(s)	2541, 4516
SstI	GAGCTC	3 cut(s)	123, 364, 1060
StuI	AGGCCT	2 cut(s)	2541, 4516
StyD4I	CCNGG	7 cut(s)	84, 346, 2313, 3248, 3657, 3847, 4029
StyI	CCWWGG	5 cut(s)	132, 1001, 2578, 2670, 4420
TaaI	ACNGT	9 cut(s)	1463, 1905, 2149, 3682, 4632, 4747, 5099, 5267, 5764
TaiI	ACGT	9 cut(s)	1317, 1452, 1570, 2338, 3165, 3203, 4191, 4353, 5461
TaqII	GACCGA	1 cut(s)	5031
TatI	WGTACW	5 cut(s)	2149, 3145, 3599, 3975, 4531
TauI	GCSGC	3 cut(s)	173, 2080, 4589
TscAI	CASTG	8 cut(s)	1606, 2710, 2941, 3745, 3923, 3962, 4948, 5029
TseFI	GTSAC	6 cut(s)	241, 1315, 1467, 2420, 2897, 5109
Tsp45I	GTSAC	6 cut(s)	241, 1315, 1467, 2420, 2897, 5109
TspGWI	ACGGA	7 cut(s)	38, 78, 2029, 2681, 2916, 3458, 4468
TspRI	CASTG	8 cut(s)	1606, 2710, 2941, 3745, 3923, 3962, 4948, 5029
Van91I	CCANNNNNTGG	3 cut(s)	868, 1002, 1928
VneI	GTGCAC	2 cut(s)	3641, 3914
VpaK11BI	GGWCC	9 cut(s)	40, 727, 980, 1836, 2644, 3889, 4253, 5095, 5556
VspI	ATTAAT	2 cut(s)	1263, 4328
XagI	CCTNNNNNAGG	1 cut(s)	5639
XbaI	TCTAGA	1 cut(s)	3091
XceI	RCATGY	5 cut(s)	1170, 1887, 3488, 3613, 3847
XcmI	CCANNNNNNNNNTGG	1 cut(s)	388
XmiI	GTMKAC	7 cut(s)	1888, 1957, 3182, 3757, 3861, 4202, 5461
XmnI	GAANNNNTTC	6 cut(s)	1668, 2016, 2268, 3432, 3573, 4150
Zsp2I	ATGCAT	1 cut(s)	2215

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.