Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr2

Orthogroup ID

OG0003911

Physical Location & Seq

Genomic Coordinates Reverse (-)

3556833 .. 3572710

Gene Structure 15878 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000006018

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5589 bp

ATGTCTCTCCCGCCCGTAGAGCGCTCACACATTAAGGAGGACCACCTCCGTGAATGGAAGAACGGAAACCCTAGCTTCAAGCTCCCGGAGCCCGTTCCGATGCTTCGGTTCCTCTACGAGCTCTGCTCCACCATGGTTCGTGGAGAATTGCCGTTTCAGAAGTGTAGAGCCGCTTTGGATTCGGTGGAGTTTACCGAAAATGTTTCGGAGCAAGAGCTGGCTTCAAGCTTGGCAGATATTGTCACTCAAATGTCTCAAGATCTTACCATGCCCGGAGAGCATCGAGCTCGTCTCACAAAACTGGCCAAATGGTTGGTGGAATCTTCTTTAGTTCCATTAAGGCTTTTTCAGGAGCGCTGTGAGGAAGAATTTTTGTGGGAGGCTGAAATGATCAAGATAAAGGCTCAAGAACTGAAGAGTAAAGAGGTTAGAGTAAACACCCGTCTTCTATATCAGCAAACAAAGTTTAATCTTCTTCGAGAAGAGAGCGAGGGATATGCCAAGCTGGTTACACTTCTTTGCCAAAATTCAGAAAATTCATCGCACAATGCAGGAGCAACAATAGGCATTATAAAGTCATTGATCGGACATTTTGATCTGGACCCAAATCATGTTTTTGATATCGTTTTGGAGTGTTTTGAACTTCTGCCGGACAACAATGTCTTTTTGGAGTTGATACCAATTTTTCCAAAGTCTCATGCTTCACAAATACTTGGTTTTAAATTTCAACATTACCAGCGTTTGGAAGTAAATGATCCTGTACCATTTGGACTTTATAAGCTTACGGCTTTGCTGGTGAAGGAGAATTTTATTGATCTTGATAGCATATGTGCTCATTTACTTCCGAAGGACGATGAGGCATTTGAGCACTACAGCTCTTTCTCCTCCAAGCAACTTGATGAGGCTAATAAAATTGGTAAAATTAATCTTGCTGCTACTGGGAAGGATCTAATGGAAGATGAGAAACAAGGAGACGTGACAATAGATCTCTTTGCATCTTTAGACATGGATTCAGAGGCAGTAGGAGAAAGATCTGCAGAGTTTGAAAATAATCAAACTTTGGGCTTGCTTACTGGTTTTCTTTCTGTAGATGACTGGTATCATGCACACATACTATTTGACCGTCTGTCACCTCTAAATCCTGTTGAACATATCCAAATCTGTAATAGCTTGTTTAGATTGATTGAAAAGTCAATCTCTTCTGCATATGACATGGTTCGTCAAGCACGTCTCAATAACTTGGGGTCATCCTCAGGAACCAGTGTTGGTGTGATGACTACAGAAAACTCATCAGCTAGTGGTTCTTTTATAGAACTTAAAAAAGAACTTTTTCAGATGCTTGCTATTGTTGGGCCTTATCTTTATCGTGATACTTTGCTGCTACAGAAGGTTTGCAGAGTATTAAGAGGTTATTACTTGTCTGCTCCGGATCTTGGAAGGACTGGTGAAGTAGCTGTTTCTGCCAGTAATCCTCGTTTGCCTCTAAAGGAAGCTAGGTCCTCCCGTCAGGTTGCAATGTCAAGGGTAGAGGAAGCCTTCCGGACATGTCTACTTCCATCTTTACAGTTAGTACCTGCAAATCCAGCTGTTGGAATGGAGATTTGGGAAGTGATGAGTCTACTTCCTTATGAGGTGAGGTATCGTTTATATGGTGAATGGGAGAAAGAAGATGAACGGATTCCTATGGTTTTAGCTGCAAGGCAAACAGCCAAGTTGGACACTAGAAGAATTTTGAAGCGGCTTGCAAAAGAAAATTTAAAGCAGCAGGGTAGGATGGTTGCAAAATTAGCTCATGCAAATCCAATGACTGTACTTCGAACAATTGTTCATCAGATCGAGGCGTACAAAGATATGATTGCTCCTGTTGTAGATGCATTTAAGTATTTGACTCAGCTGGAGTATGATGTCCTGGAATATGTTGTCATTGAACGTCTGGCGCAAGGAGGGCGTGATAAGCTAAAAGAGGATGGGCTTAACTTGTCAGACTGGCTTCAATCTTTGGCTTCATTTTGGGGTCACTTGTGTAAAAAATATCCATCTATGGAATTGAGAGGCCTTTTCCAGTATCTTGTAAATCAGTTGAAAAAGGGCCAAGGAATTGAGCTTGTTGTTCTTCAGGAGCTTATTCAGCAAATGGCAAATGTCCATCACACAGAGGACCTTACTGAAGATCAGCTGGATGCCATGGCAGGGGGTGAGACTCTCCGTCATCACGCCACTGGTTTTGGTGTTACTCGACATAACAAGCAACTAATCAAGTCCACAAACAGGCTAAGGGACTCACTGCTTCCAAAGGATGAAACCAAGCTGGCAATCCCTCTTTTGTTACATCTTGCTCAGCATCATTCTTTGGTCATAATAGATGCAGATGCGCCTTATATTAAGATGGTTAGCGAGCAGTTTGATAGATGTCACGGAGCACTCCTTCAGTATGTGGAATTTCTTTGCAGTGCCATGCCATCGGCATCAGCTTATGCTCAGCTAATTCCTTCACTTGATGATCTTGTCCATAAATACCACCTTGATCCTGAGGTTGCTTTCTTAATTTATCGCCCAGTAATGAGGCTCTTCAAGAGCCCCGCGAGTTCTGATGTCTTCTGGCCTCTAGACAATAATGATGCTCAAAGTATCACATCTGCAAATTCAGAGTTTGAAGCTGTACAGAACTCTGGCAACGTGGTTCTGGATCTCGGGTCTACATGGAACCCTATAACGTGGTTGGATCTTCTTCATACTGCTAAAACTATGTTGCCTGCAAGAGCCTGGAATAGTTTGTCCCCTGATCTTTATGCTACATTTTGGGGGCTCACACTCTACGATCTTTATGTCCCTAGAAATTGTTATGAATCTGAAATTGCGAAACAGCAGGCTTCTCTTAAAGTTCTGGAAGAGCAGCCTGATAATTCAATTTCTGCAATAACCAAAAGGAAGAAAGAGAAGGAAAGAATTCAAGATACCATAGATCGCCTGACTAGTGAACTCCGTAAACATGAGGAACATGTTGCATCTGTTCGTAAACGCTTATCGCGTGAGAAGGACAAATGGTTGAGTTCTTGTCTTGATACTTTAAAGATCAACATGGAATTCCTTCAGCGTTGTATATTTCCTCGCTGTACTTTCAGCATGCCAGATGCTGTCTATTGTGCCATGTTTGTGCACACTCTTCATTCCCTGGGGACACCATTTTTTAACACAGTCAACCACATGGATGTTCTTATATGTAGAACCTTACAGCCTATGATATGCTGCTGCACTGAATCTGAAGTTGGTAGACTGGGGAAGTTTCTCTGTGAGACCTTGAAAATTGCTTACTATTGGAAGAGTGATGAATCTATTTATGAGCGCGAGTGTGGAAACATGCCAGGATTTGCTGTCTACTATAGGTTTCCAGATAGCCAGCGGGTCCGATATGGCCAGTTTGTCAAGGTGCACTGGAAATGGAGTCAAAGGATCACGAGATTATTGGGTCAGTGTCTGGAATCTCCGGAGTACATGGAAATTCGAAACGCTCTCATAATCTTATCAAGAATTTCTAGTGTTTTCCCAGTTACTAAAAAGAGTGGGATGAACCTTGAAAGACGGGTGTCCAAGATCAAAGGTGATGGGAGAGAGGACCTCAAGGTGTTGGCAACAAGTGTTGGTGCTTCTTTGGCTGCTAGAAAGCCTTCATTGGTTTCGGATGAAGAATTTTGTATGGGATACGTTGAATTAAAGTCGACAGCATCTTCAAAGCCTTCGGCCAGTAATTCAGGACCTATACAGAGTGGTTCTGCCGGTAATAATTCTCAAACTGAACCTGTTGGCGGAAGAGCAGGAACCCTTGTCTCCCAACACTCAGAATTAATAAACTCAGCCAGAGACCATGTGTCAAAAACCAAACCTGCAGATGGGAGGTTGGAAAGAGCAGAAAGTATCTCAACTGCAAAATCTGACCAAGGGCATCTAAAACTGAAAGGTGCCTCATTGGTGAATGGCTCAGATGCTCAGGCATCTGTGCCATCAGCTAGTTTACAAACTGGAACATCAAGGCCTATCGAAAATCAAGTACAAGTGAACGAAATCTCAAATCGAACGCCAGATGAAAATATGGGAAAACTTGCTGCAAAGAACACTTCAGAATCTGAGTTGAGGGCCCAAGCAAAACGGTCTGTTCCTGCTGGGGCTAAGCCATTGAAACAAGATCTTGCGAAGGACGAAAGTAGATCTGGAAAAGCAGCTGGAGCTACTAATGCTTCTTCCATCACCGCAAACGGTAGCACGATATCATCCTCGGGCAAAGGTTCAGCTTCGTTAGGTATTGAATCAAAAGTGGAGGCTGGATCTGCAAAGTTTTCCAATACAAGGATCCCTTCTTCAAAGGAAGAGGGTGCTGAGGTTTCTGATGCAGCAAGGCCCCCTTCTTCTCGATTCGTTCATTCTCCTAGACATGATAGTTCTGCTACACTCTCAAAGTCCAGTGATAAACTTCAAAAAAGAGCCAGCCCTGCAGAAGAAACGGATAGACAAAGTAAACGCCGGAAGGGGGAGGCTGAAATGAAAGATTTTGAGGGTGAAGCTCGCTTGTCTGACCGAGAGAGGTCCGTTGATGCTAGACTGTTGGATCTTGACAAATCAGGAAGCGATGATAGAAGTGTATATAAGGCCACTGAAAAGGCTTCAGATCGGTCTAAAGATAAAGGAAGTGAAAGACATGATAAGGATCACAGGGAAAGATTGGACCGTCCTGATAAGTCACGCGGTGATGATTTAGTTGAGAGATCCAGAGATAGGTCAATGGAGAGACATGGAAGGGATCATTCAGCTGAGAAATTGCAAGAGAGGGGTACAGATAGGAGTTTTGATAGACTGCCTGACAAATCTAAGGATGAGAAAGGTAAAGGGCGTTACAGTGATATTTCTGCTGAAAAATCTCATGTTGATGATCGATATCACGGGCAAAGCTTACCTCCACCACCACCCTTACCTCCTCACATTGTTCCTCAATCTGTTAGTTCTGGAAGAAGAGATGAAGATTCAGATAGAAGGACTGCAACTACAAGGCATACTCAAAGGCTTTCTCCGAGACATGATGAGAAGGAACGTAGACGATCTGAAGAAAATTCCTCAATTTCTCAAGATGATTCAAAGCGTAGAAGAGAAGATGATTTTCGAGAGAGAAAGCGTGATGATCGTGAGGGGACCTCAGTAAAGGTGGAAGAGAGGGACAGGGAGAGGGAAAGGGAGCGGGAGAGGGAGAAAGAAAGGGAGAAAGTCAACCTCTTGAAGGAGGATTCAGACTCAATTGCAGCTTCTAAGAGGCGGAAACTTAAACGAGAACATCTGGCTTCTGGGGAAGCTGGCGAGTACTCACCAGTTCACCCTCCCCCTCCCCTCTCTATTAACTTGTCCCAGTCATACGATGGAAGAGATAGGGGAGAACGGAAAGGACCCATTGTCACGCGCACAGGTTACATGGAGGAACCAAGTCTGAGGATTCATGGTAAAGAAGTATCCAGCAAGATGACCCGCCGTGATACAGACCCGATGTACGACTGGGACGACGATAAAAGGCAAAGAGGTGAACAGAAACGAAGGCATCGGAAGAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000346 GO:0000347 GO:0000445 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006396 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0008104 GO:0008150 GO:0008152 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0015031 GO:0015833 GO:0015931 GO:0016070 GO:0016441 GO:0016458 GO:0016604 GO:0016607 GO:0019222 GO:0031047 GO:0031050 GO:0031503 GO:0031974 GO:0031981 GO:0032991 GO:0033036 GO:0034613 GO:0034641 GO:0035194 GO:0035195 GO:0035196 GO:0040029 GO:0042221 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048519 GO:0050657 GO:0050658 GO:0050789 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070727 GO:0070887 GO:0070918 GO:0071166 GO:0071310 GO:0071359 GO:0071407 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1990428

KEGG Pathways

Metabolic & Signaling

ko03013 ko03040 map03013 map03040

Pfam Domains

Protein Families

THOC2_N Tho2 Thoc2

Protein Analysis

1863

Amino Acids

210.69

Weight (kDa)

7.32

Isoelectric Point (pI)

47.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
THOC2_N	PF16134	37 - 413	1.9e-45	THO complex subunit 2 N-terminus
THOC2_N	PF16134	442 - 591	3e-21	THO complex subunit 2 N-terminus
Thoc2	PF11732	593 - 668	9.1e-30	Transcription- and export-related complex subunit
Tho2	PF11262	922 - 1216	4.9e-96	Transcription factor/nuclear export subunit protein 2

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0003911)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G24706 AT1G24706 AT1G24706 AT1G24706 AT1G24706 AT1G24706 AT1G24706 AT1G24706 AT1G24706
fragaria_vesca	FvH4_4g33200 FvH4_4g33200 FvH4_4g33200
malus_domestica	MD09G1258800.v1.1
prunus_persica	Prupe.3G150800_v2.0.a1 Prupe.3G150800_v2.0.a1 Prupe.3G150800_v2.0.a1 Prupe.3G150800_v2.0.a1 Prupe.3G150800_v2.0.a1 Prupe.3G150800_v2.0.a1 Prupe.3G150800_v2.0.a1 Prupe.3G150800_v2.0.a1 Prupe.3G150800_v2.0.a1
pyrus_communis	pycom09g17360
rosa_chinensis	RchiOBHm_Chr4g0442311
rosa_laevigata	RLG00000006018
rosa_multiflora	Rmu_sc0000124.1_g000055
rosa_roxburghii	Rroxscaffold_5G00383170
rosa_rugosa	Rorug04G0340300 Rorug04G0340400
rosa_samantha	Rh4AG391900 Rh4BG405400 Rh4CG420600 Rh4DG398700
rosa_wichuraiana	Rw4G033850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	572, 777
AasI	GACNNNNNNGTC	1 cut(s)	659
Acc36I	ACCTGC	2 cut(s)	1582, 3889
AccB1I	GGYRCC	1 cut(s)	3956
AccB7I	CCANNNNNTGG	2 cut(s)	742, 1589
AccBSI	CCGCTC	1 cut(s)	5224
AccI	GTMKAC	7 cut(s)	1549, 1618, 2696, 3271, 3375, 3716, 5083
AccII	CGCG	5 cut(s)	2582, 3028, 3345, 4737, 5440
AccIII	TCCGGA	3 cut(s)	1426, 1539, 3484
AcoI	YGGCCR	3 cut(s)	303, 3412, 3738
AcuI	CTGAAG	9 cut(s)	434, 2099, 2187, 2411, 3074, 3282, 4098, 4641, 5112
AdeI	CACNNNGTG	1 cut(s)	4739
AfeI	AGCGCT	2 cut(s)	23, 356
AflIII	ACRYGT	2 cut(s)	1544, 2998
AhdI	GACNNNNNGTC	4 cut(s)	1126, 2205, 3865, 5432
AhlI	ACTAGT	1 cut(s)	2972
AjiI	CACGTC	2 cut(s)	976, 1229
AjnI	CCWGG	4 cut(s)	1908, 2762, 3171, 3361
AjuI	GAANNNNNNNTTGG	4 cut(s)	1585, 1617, 3249, 3281
AleI	CACNNNNGTG	1 cut(s)	48
Alw21I	GWGCWC	7 cut(s)	123, 289, 835, 870, 2422, 3159, 3432
Alw44I	GTGCAC	2 cut(s)	3155, 3428
AlwNI	CAGNNNCTG	3 cut(s)	3134, 3475, 4121
Ama87I	CYCGRG	2 cut(s)	2690, 4270
Aor13HI	TCCGGA	3 cut(s)	1426, 1539, 3484
Aor51HI	AGCGCT	2 cut(s)	23, 356
ApaI	GGGCCC	1 cut(s)	4135
ApaLI	GTGCAC	2 cut(s)	3155, 3428
AseI	ATTAAT	2 cut(s)	924, 3842
Asp700I	GAANNNNTTC	5 cut(s)	1329, 1677, 2946, 3087, 3664
AspLEI	GCGC	6 cut(s)	24, 357, 1939, 2374, 3345, 5442
AsuC2I	CCSGG	2 cut(s)	86, 273
AsuII	TTCGAA	2 cut(s)	1816, 3502
AvaI	CYCGRG	2 cut(s)	2690, 4270
AxyI	CCTNAGG	2 cut(s)	1252, 2529
BaeGI	GKGCMC	3 cut(s)	3159, 3432, 4135
BalI	TGGCCA	2 cut(s)	305, 3414
BamHI	GGATCC	1 cut(s)	4344
BanI	GGYRCC	1 cut(s)	3956
BanII	GRGCYC	6 cut(s)	93, 123, 289, 2579, 2808, 4135
BauI	CACGAG	1 cut(s)	3454
BbsI	GAAGAC	2 cut(s)	437, 2587
Bbv12I	GWGCWC	7 cut(s)	123, 289, 835, 870, 2422, 3159, 3432
BbvCI	CCTCAGC	1 cut(s)	4371
BceAI	ACGGC	3 cut(s)	136, 801, 5493
BcgI	CGANNNNNNTGC	4 cut(s)	269, 303, 479, 513
BciT130I	CCWGG	4 cut(s)	1910, 2764, 3173, 3363
BciVI	GTATCC	2 cut(s)	3692, 5500
BclI	TGATCA	1 cut(s)	390
BcnI	CCSGG	2 cut(s)	86, 273
BcuI	ACTAGT	1 cut(s)	2972
BfmI	CTRYAG	8 cut(s)	871, 1035, 1086, 1278, 1382, 3379, 3882, 4485
BfoI	RGCGCY	2 cut(s)	25, 358
BfuAI	ACCTGC	2 cut(s)	1582, 3889
BfuI	GTATCC	2 cut(s)	3692, 5500
BglII	AGATCT	5 cut(s)	259, 985, 1031, 4180, 4202
BlpI	GCTNAGC	3 cut(s)	2337, 2478, 4164
BmcAI	AGTACT	1 cut(s)	5345
Bme1390I	CCNGG	6 cut(s)	86, 273, 1910, 2764, 3173, 3363
BmeRI	GACNNNNNGTC	4 cut(s)	1126, 2205, 3865, 5432
BmeT110I	CYCGRG	2 cut(s)	2690, 4270
BmgBI	CACGTC	2 cut(s)	976, 1229
BmrFI	CCNGG	6 cut(s)	86, 273, 1910, 2764, 3173, 3363
BmrI	ACTGGG	6 cut(s)	948, 2549, 3284, 3539, 5383, 5542
BmuI	ACTGGG	6 cut(s)	948, 2549, 3284, 3539, 5383, 5542
BoxI	GACNNNNGTC	1 cut(s)	3583
BpiI	GAAGAC	2 cut(s)	437, 2587
BplI	GAGNNNNNCTC	8 cut(s)	110, 142, 276, 308, 3600, 3632, 5165, 5197
BpmI	CTGGAG	2 cut(s)	1916, 4239
Bpu10I	CCTNAGC	3 cut(s)	2273, 3984, 4371
Bpu1102I	GCTNAGC	3 cut(s)	2337, 2478, 4164
Bpu14I	TTCGAA	2 cut(s)	1816, 3502
BpuEI	CTTGAG	4 cut(s)	240, 390, 3602, 5097
BpuMI	CCSGG	2 cut(s)	86, 273
Bsa29I	ATCGAT	1 cut(s)	4924
BsaBI	GATNNNNATC	2 cut(s)	4698, 4926
BsaI	GGTCTC	2 cut(s)	3287, 3852
BsaJI	CCNNGG	7 cut(s)	132, 2092, 2184, 3171, 3172, 3934, 4269
BsaWI	WCCGGW	3 cut(s)	1426, 1539, 3484
Bse118I	RCCGGY	1 cut(s)	3773
Bse21I	CCTNAGG	2 cut(s)	1252, 2529
Bse3DI	GCAATG	1 cut(s)	1521
Bse8I	GATNNNNATC	2 cut(s)	4698, 4926
BseAI	TCCGGA	3 cut(s)	1426, 1539, 3484
BseBI	CCWGG	4 cut(s)	1910, 2764, 3173, 3363
BseCI	ATCGAT	1 cut(s)	4924
BseDI	CCNNGG	7 cut(s)	132, 2092, 2184, 3171, 3172, 3934, 4269
BseJI	GATNNNNATC	2 cut(s)	4698, 4926
BseMI	GCAATG	1 cut(s)	1521
BseRI	GAGGAG	2 cut(s)	874, 4956
BseSI	GKGCMC	3 cut(s)	3159, 3432, 4135
BseYI	CCCAGC	1 cut(s)	4157
BsgI	GTGCAG	1 cut(s)	3235
Bsh1236I	CGCG	5 cut(s)	2582, 3028, 3345, 4737, 5440
BshNI	GGYRCC	1 cut(s)	3956
BshVI	ATCGAT	1 cut(s)	4924
BsiHKAI	GWGCWC	7 cut(s)	123, 289, 835, 870, 2422, 3159, 3432
BsiHKCI	CYCGRG	2 cut(s)	2690, 4270
BsiSI	CCGG	8 cut(s)	86, 273, 650, 1427, 1540, 3485, 3774, 4516
BslFI	GGGAC	8 cut(s)	2291, 2761, 2813, 3190, 5191, 5216, 5371, 5549
BsmBI	CGTCTC	3 cut(s)	296, 966, 1235
BsmFI	GGGAC	8 cut(s)	2291, 2761, 2813, 3190, 5191, 5216, 5371, 5549
Bso31I	GGTCTC	2 cut(s)	3287, 3852
BsoBI	CYCGRG	2 cut(s)	2690, 4270
Bsp119I	TTCGAA	2 cut(s)	1816, 3502
Bsp120I	GGGCCC	1 cut(s)	4131
Bsp13I	TCCGGA	3 cut(s)	1426, 1539, 3484
Bsp1407I	TGTACA	1 cut(s)	2659
Bsp1720I	GCTNAGC	3 cut(s)	2337, 2478, 4164
Bsp19I	CCATGG	2 cut(s)	132, 2184
BspDI	ATCGAT	1 cut(s)	4924
BspEI	TCCGGA	3 cut(s)	1426, 1539, 3484
BspFNI	CGCG	5 cut(s)	2582, 3028, 3345, 4737, 5440
BspMAI	CTGCAG	3 cut(s)	1039, 3886, 4489
BspMI	ACCTGC	2 cut(s)	1582, 3889
BspQI	GCTCTTC	3 cut(s)	2573, 2883, 3802
BspT104I	TTCGAA	2 cut(s)	1816, 3502
BspT107I	GGYRCC	1 cut(s)	3956
BspTNI	GGTCTC	2 cut(s)	3287, 3852
BsrBI	CCGCTC	1 cut(s)	5224
BsrDI	GCAATG	1 cut(s)	1521
BsrFI	RCCGGY	1 cut(s)	3773
BsrGI	TGTACA	1 cut(s)	2659
BssAI	RCCGGY	1 cut(s)	3773
BssECI	CCNNGG	7 cut(s)	132, 2092, 2184, 3171, 3172, 3934, 4269
BssSI	CACGAG	1 cut(s)	3454
BssT1I	CCWWGG	4 cut(s)	132, 2092, 2184, 3934
Bst2BI	CACGAG	1 cut(s)	3454
Bst2UI	CCWGG	4 cut(s)	1910, 2764, 3173, 3363
Bst4CI	ACNGT	9 cut(s)	1124, 1566, 1810, 3196, 4146, 4253, 4596, 4721, 4889
BstAUI	TGTACA	1 cut(s)	2659
BstBI	TTCGAA	2 cut(s)	1816, 3502
BstDSI	CCRYGG	2 cut(s)	132, 2184
BstENI	CCTNNNNNAGG	1 cut(s)	5261
BstFNI	CGCG	5 cut(s)	2582, 3028, 3345, 4737, 5440
BstH2I	RGCGCY	2 cut(s)	25, 358
BstHHI	GCGC	6 cut(s)	24, 357, 1939, 2374, 3345, 5442
BstNI	CCWGG	4 cut(s)	1910, 2764, 3173, 3363
BstNSI	RCATGY	4 cut(s)	1548, 3002, 3127, 3361
BstPAI	GACNNNNGTC	1 cut(s)	3583
BstSCI	CCNGG	6 cut(s)	84, 271, 1908, 2762, 3171, 3361
BstSFI	CTRYAG	8 cut(s)	871, 1035, 1086, 1278, 1382, 3379, 3882, 4485
BstSLI	GKGCMC	3 cut(s)	3159, 3432, 4135
BstUI	CGCG	5 cut(s)	2582, 3028, 3345, 4737, 5440
BstV2I	GAAGAC	2 cut(s)	437, 2587
BstXI	CCANNNNNNTGG	1 cut(s)	313
Bsu15I	ATCGAT	1 cut(s)	4924
Bsu36I	CCTNAGG	2 cut(s)	1252, 2529
BsuI	GTATCC	2 cut(s)	3692, 5500
BsuTUI	ATCGAT	1 cut(s)	4924
BtgI	CCRYGG	2 cut(s)	132, 2184
BtgZI	GCGATG	2 cut(s)	525, 4635
BtrI	CACGTC	2 cut(s)	976, 1229
BtsI	GCAGTG	2 cut(s)	2282, 2455
BveI	ACCTGC	2 cut(s)	1582, 3889
CaiI	CAGNNNCTG	3 cut(s)	3134, 3475, 4121
CfoI	GCGC	6 cut(s)	24, 357, 1939, 2374, 3345, 5442
Cfr10I	RCCGGY	1 cut(s)	3773
ClaI	ATCGAT	1 cut(s)	4924
CspCI	CAANNNNNGTGG	2 cut(s)	2206, 2241
DraI	TTTAAA	3 cut(s)	721, 1758, 3069
DraIII	CACNNNGTG	1 cut(s)	4739
DrdI	GACNNNNNNGTC	1 cut(s)	659
DriI	GACNNNNNGTC	4 cut(s)	1126, 2205, 3865, 5432
DseDI	GACNNNNNNGTC	1 cut(s)	659
EaeI	YGGCCR	3 cut(s)	303, 3412, 3738
Eam1105I	GACNNNNNGTC	4 cut(s)	1126, 2205, 3865, 5432
EciI	GGCGGA	2 cut(s)	3819, 5314
Ecl136II	GAGCTC	2 cut(s)	121, 287
Eco130I	CCWWGG	4 cut(s)	132, 2092, 2184, 3934
Eco147I	AGGCCT	2 cut(s)	2055, 4030
Eco24I	GRGCYC	6 cut(s)	93, 123, 289, 2579, 2808, 4135
Eco31I	GGTCTC	2 cut(s)	3287, 3852
Eco32I	GATATC	3 cut(s)	622, 4263, 4928
Eco47III	AGCGCT	2 cut(s)	23, 356
Eco53kI	GAGCTC	2 cut(s)	121, 287
Eco57I	CTGAAG	9 cut(s)	434, 2099, 2187, 2411, 3074, 3282, 4098, 4641, 5112
Eco81I	CCTNAGG	2 cut(s)	1252, 2529
Eco88I	CYCGRG	2 cut(s)	2690, 4270
EcoICRI	GAGCTC	2 cut(s)	121, 287
EcoNI	CCTNNNNNAGG	1 cut(s)	5261
EcoO109I	RGGNCCY	8 cut(s)	1497, 2158, 3613, 3752, 4131, 4392, 5178, 5426
EcoRI	GAATTC	2 cut(s)	2946, 3083
EcoRII	CCWGG	4 cut(s)	1908, 2762, 3171, 3361
EcoRV	GATATC	3 cut(s)	622, 4263, 4928
EcoT14I	CCWWGG	4 cut(s)	132, 2092, 2184, 3934
EcoT22I	ATGCAT	1 cut(s)	1876
EcoT38I	GRGCYC	6 cut(s)	93, 123, 289, 2579, 2808, 4135
ErhI	CCWWGG	4 cut(s)	132, 2092, 2184, 3934
Esp3I	CGTCTC	3 cut(s)	296, 966, 1235
FalI	AAGNNNNNCTT	4 cut(s)	4333, 4365, 4381, 4413
FaqI	GGGAC	8 cut(s)	2291, 2761, 2813, 3190, 5191, 5216, 5371, 5549
FauI	CCCGC	5 cut(s)	18, 2587, 3393, 5217, 5513
FauNDI	CATATG	2 cut(s)	827, 1207
FbaI	TGATCA	1 cut(s)	390
FblI	GTMKAC	7 cut(s)	1549, 1618, 2696, 3271, 3375, 3716, 5083
FriOI	GRGCYC	6 cut(s)	93, 123, 289, 2579, 2808, 4135
GlaI	GCGC	6 cut(s)	23, 356, 1938, 2373, 3344, 5441
GsaI	CCCAGC	1 cut(s)	4161
GsuI	CTGGAG	2 cut(s)	1916, 4239
HaeII	RGCGCY	2 cut(s)	25, 358
HapII	CCGG	8 cut(s)	86, 273, 650, 1427, 1540, 3485, 3774, 4516
HhaI	GCGC	6 cut(s)	24, 357, 1939, 2374, 3345, 5442
Hin6I	GCGC	6 cut(s)	22, 355, 1937, 2372, 3343, 5440
HinP1I	GCGC	6 cut(s)	22, 355, 1937, 2372, 3343, 5440
HincII	GTYRAC	3 cut(s)	3199, 3717, 5254
HindII	GTYRAC	3 cut(s)	3199, 3717, 5254
HindIII	AAGCTT	3 cut(s)	226, 779, 4939
HpaII	CCGG	8 cut(s)	86, 273, 650, 1427, 1540, 3485, 3774, 4516
Hpy99I	CGWCG	1 cut(s)	5543
HpyCH4III	ACNGT	9 cut(s)	1124, 1566, 1810, 3196, 4146, 4253, 4596, 4721, 4889
HpyCH4IV	ACGT	7 cut(s)	975, 1228, 1930, 2676, 2714, 3702, 5080
HpySE526I	ACGT	7 cut(s)	975, 1228, 1930, 2676, 2714, 3702, 5080
HspAI	GCGC	6 cut(s)	22, 355, 1937, 2372, 3343, 5440
Kpn2I	TCCGGA	3 cut(s)	1426, 1539, 3484
Ksp22I	TGATCA	1 cut(s)	390
LguI	GCTCTTC	3 cut(s)	2573, 2883, 3802
MaeII	ACGT	7 cut(s)	975, 1228, 1930, 2676, 2714, 3702, 5080
MbiI	CCGCTC	1 cut(s)	5224
MfeI	CAATTG	2 cut(s)	1821, 5280
MlsI	TGGCCA	2 cut(s)	305, 3414
MluNI	TGGCCA	2 cut(s)	305, 3414
MlyI	GAGTC	6 cut(s)	1624, 1882, 2194, 2273, 3451, 5270
MmeI	TCCRAC	5 cut(s)	1570, 1695, 2700, 3876, 4578
Mox20I	TGGCCA	2 cut(s)	305, 3414
Mph1103I	ATGCAT	1 cut(s)	1876
MroI	TCCGGA	3 cut(s)	1426, 1539, 3484
MroXI	GAANNNNTTC	5 cut(s)	1329, 1677, 2946, 3087, 3664
MscI	TGGCCA	2 cut(s)	305, 3414
MslI	CAYNNNNRTG	5 cut(s)	48, 248, 3152, 3207, 4917
Msp20I	TGGCCA	2 cut(s)	305, 3414
MspA1I	CMGCKG	6 cut(s)	1586, 1894, 2176, 3400, 4217, 4802
MspI	CCGG	8 cut(s)	86, 273, 650, 1427, 1540, 3485, 3774, 4516
MspR9I	CCNGG	6 cut(s)	86, 273, 1910, 2764, 3173, 3363
MunI	CAATTG	2 cut(s)	1821, 5280
MvaI	CCWGG	4 cut(s)	1910, 2764, 3173, 3363
MvnI	CGCG	5 cut(s)	2582, 3028, 3345, 4737, 5440
NciI	CCSGG	2 cut(s)	86, 273
NcoI	CCATGG	2 cut(s)	132, 2184
NdeI	CATATG	2 cut(s)	827, 1207
NmuCI	GTSAC	7 cut(s)	241, 976, 1128, 2015, 2411, 4731, 5434
NsiI	ATGCAT	1 cut(s)	1876
NspI	RCATGY	4 cut(s)	1548, 3002, 3127, 3361
NspV	TTCGAA	2 cut(s)	1816, 3502
OliI	CACNNNNGTG	1 cut(s)	48
PaeI	GCATGC	1 cut(s)	3127
PasI	CCCWGGG	1 cut(s)	3172
PceI	AGGCCT	2 cut(s)	2055, 4030
PciI	ACATGT	2 cut(s)	1544, 2998
PciSI	GCTCTTC	3 cut(s)	2573, 2883, 3802
PcsI	WCGNNNNNNNCGW	3 cut(s)	1225, 3025, 5535
PdmI	GAANNNNTTC	5 cut(s)	1329, 1677, 2946, 3087, 3664
PflMI	CCANNNNNTGG	2 cut(s)	742, 1589
PfoI	TCCNGGA	2 cut(s)	84, 1908
PleI	GAGTC	6 cut(s)	1623, 1882, 2194, 2273, 3450, 5270
PpsI	GAGTC	6 cut(s)	1623, 1882, 2194, 2273, 3450, 5270
PpuMI	RGGWCCY	6 cut(s)	1497, 2158, 3613, 3752, 5178, 5426
PscI	ACATGT	2 cut(s)	1544, 2998
PshAI	GACNNNNGTC	1 cut(s)	3583
PshBI	ATTAAT	2 cut(s)	924, 3842
PsiI	TTATAA	2 cut(s)	572, 777
Psp124BI	GAGCTC	2 cut(s)	123, 289
Psp5II	RGGWCCY	6 cut(s)	1497, 2158, 3613, 3752, 5178, 5426
Psp6I	CCWGG	4 cut(s)	1908, 2762, 3171, 3361
PspFI	CCCAGC	1 cut(s)	4157
PspGI	CCWGG	4 cut(s)	1908, 2762, 3171, 3361
PspOMI	GGGCCC	1 cut(s)	4131
PspPPI	RGGWCCY	6 cut(s)	1497, 2158, 3613, 3752, 5178, 5426
PstI	CTGCAG	3 cut(s)	1039, 3886, 4489
PstNI	CAGNNNCTG	3 cut(s)	3134, 3475, 4121
PvuII	CAGCTG	5 cut(s)	1586, 1894, 2176, 4217, 4802
RseI	CAYNNNNRTG	5 cut(s)	48, 248, 3152, 3207, 4917
SacI	GAGCTC	2 cut(s)	123, 289
SalI	GTCGAC	1 cut(s)	3715
SapI	GCTCTTC	3 cut(s)	2573, 2883, 3802
ScaI	AGTACT	1 cut(s)	5345
SchI	GAGTC	6 cut(s)	1624, 1882, 2194, 2273, 3451, 5270
ScrFI	CCNGG	6 cut(s)	86, 273, 1910, 2764, 3173, 3363
SfcI	CTRYAG	8 cut(s)	871, 1035, 1086, 1278, 1382, 3379, 3882, 4485
SfuI	TTCGAA	2 cut(s)	1816, 3502
SmiMI	CAYNNNNRTG	5 cut(s)	48, 248, 3152, 3207, 4917
SmlI	CTYRAG	4 cut(s)	255, 405, 3617, 5112
SmoI	CTYRAG	4 cut(s)	255, 405, 3617, 5112
SpeI	ACTAGT	1 cut(s)	2972
SphI	GCATGC	1 cut(s)	3127
SseBI	AGGCCT	2 cut(s)	2055, 4030
SstI	GAGCTC	2 cut(s)	123, 289
StuI	AGGCCT	2 cut(s)	2055, 4030
StyD4I	CCNGG	6 cut(s)	84, 271, 1908, 2762, 3171, 3361
StyI	CCWWGG	4 cut(s)	132, 2092, 2184, 3934
TaaI	ACNGT	9 cut(s)	1124, 1566, 1810, 3196, 4146, 4253, 4596, 4721, 4889
TaiI	ACGT	7 cut(s)	978, 1231, 1933, 2679, 2717, 3705, 5083
TaqII	GACCGA	2 cut(s)	4584, 4653
TatI	WGTACW	6 cut(s)	1810, 2659, 3113, 3489, 4045, 5343
TauI	GCSGC	2 cut(s)	173, 1741
TseFI	GTSAC	7 cut(s)	241, 976, 1128, 2015, 2411, 4731, 5434
Tsp45I	GTSAC	7 cut(s)	241, 976, 1128, 2015, 2411, 4731, 5434
TspGWI	ACGGA	9 cut(s)	38, 78, 1690, 2195, 2430, 2972, 4511, 4570, 5434
Van91I	CCANNNNNTGG	2 cut(s)	742, 1589
VneI	GTGCAC	2 cut(s)	3155, 3428
VspI	ATTAAT	2 cut(s)	924, 3842
XagI	CCTNNNNNAGG	1 cut(s)	5261
XbaI	TCTAGA	1 cut(s)	2605
XceI	RCATGY	4 cut(s)	1548, 3002, 3127, 3361
XcmI	CCANNNNNNNNNTGG	2 cut(s)	313, 5396
XmiI	GTMKAC	7 cut(s)	1549, 1618, 2696, 3271, 3375, 3716, 5083
XmnI	GAANNNNTTC	5 cut(s)	1329, 1677, 2946, 3087, 3664
ZrmI	AGTACT	1 cut(s)	5345
Zsp2I	ATGCAT	1 cut(s)	1876

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.