Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0003911

Physical Location & Seq

Genomic Coordinates Reverse (-)

33080480 .. 33100392

Gene Structure 19913 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1258800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5613 bp

ATGTCTCTCCCCCCCGTAGAGCGTGCGTACGTCACAGATGACTGCCTTCGTCAATGGAAGAATGGAAACCCTAGTTTCAAGCTCCCGGACCCGGTTCCGATGCTTCGATTTCTCTACGAGCTCTGCTCCACCATGGTATGTGGGGAACTGCCGCTTCACAAGTGTAAAGCGGCCTTGGATTCGGTTGAGTTCTCCGACGAGGTTTCGGACCAAGACTTGCCTTCCTGTTTTGTGGATATCATCACCCAATTGTCTCAAGATATTAGAATGCCTGGAGAGGATCGTGCTCGTCTGGTAAAATTGGCCAAATGGTTGGTGGAATCTTCCTTAGTTCCGTTGAGACTTTTCCAGGAGCGCTGTGAGGAAGAATTTTTGTGGGAGGCTGAAATGATCAAGATAAAGGCTCAGGAATTGAAGAGTAAAGAGGTCAGAGTAAATACCCGTCTTCTATATCAGCAAACAAAATTTAACCTTCTCCGAGAAGAGAGCGAGGGCTATGCCAAGCTGGTTACTCTTCTTTGCCAAAACTCAGAAACTTCATCACACAATGCAGCAGCAACAATAGGAATTATAAAGTCATTAATTGGACATTTTGATCTGGACCCAAATCATGTTTTCGATATTGTTTTGGAGTGCTTTGAACTTCAACCAGATAGTGATGTCTTTTTGGAGCTGATACCAATTTTTCCAAAGTCTCATGCTTCACAAGTCCTTGGTTTCAAATTTCAATACTATCAGCGCTTGGAAGTAAATAGTCCAGTACCATTTGGACTTTATAAGCTAACAGCTTTGCTGGTGAAAGAGGACTTTATTGATCTTGATAGCATATATGCTCATCTACTTCCCAAGGACAATGAGGCATTTGAGCATTATAGTGCTTTCTCTTCCAAGCGACTTGATGAGGCTAACAAAATTGGTAAAATAAATCTTGCTGCAACTGGGAAGGATCTGATGGATGATGAGAAACAAGGAGATGTGACAATAGATTTTTTTGCCGCTTTAGACATGGAAACAGGTGCAGTTTGTGAGCGGTCTGCAGAGTGTGAAAATAATCAAACTTTGGGCTTGCTTACTGGTTTTCTTTCCGTAGATGACTGGTACCATGCACATCTACTATTTGAGCGGCTCTCACCTCTCCATCCTGTTGAACATATTCAAATCTGCGACAGCTTGTTTAGGCTCATTGAAAAATCAATCTCTTCTGCTTATGACACTGTTCGTCGAGCACATCTTCCAATATTTGGGGCTTCCTCAGGAACTAGTATTGATGTGATGAATACAGAGAATGCATCAATCCATGGATCATTTATAGAACTTCCAAAAGAACTTTTTCAGATGCTTACTTGTGCTGGACCATATCTTTATCGTAATACTTTGTTGCTGCAGAAGGTCTGTAGAGTATTGAGAGGTTATTACTCGTCTGCGCTTCAGCTTGTAAACAGTGGTGAAGGAGTCATAGATCCCAATCATGTTTTTGGAGGTAATCCTCGTCTGCATCTTAAGGAAGCAAGGTCAAGGATAGAGGAAGCTTTGGGAACATGTCTACTTCCTTCATTACAATTGGTACCTGCAAATCCAGCTGTTGGACAGGAGATCTGGGAAGTGATGAATCTGCTTCCCTATGAGGTGCGGTATCGTTTATATGGTGAGTGGGAGAAAGAAGATGAGCAGATTCCTATGGTTTTAGCTGCAAGACAAACAGCCAAGTTGGACACAAGAAGAATTTTGAAACGGCTTGCAAAAGAAAATTTGAAGCAGCTGGGTCGGATGGTTGCAAAACTGGCTCACGCAAATCCAATGACTGTACTAAGAACAATTGTTCATCAGATTGAGGCGTACAGAGATATGATTGCTCCTGTTGTAGATGCATTTAAGTATTTGACTCAGCTGGAGTATGACGTTTTGGAATATGTTGTCATTGAGCGTCTGGCTCAAGGAGGTCGTGACAAGCTAAAAGATGATGGCCTTAATTTGTCAGACTGGCTTCAATCTTTGGCTTCATTTTGGGGTCACTTGTGTAAGAAATACCCATCTATGGAATTGAGGGGGCTTTTCCAGTATCTTGTGAACCAACTAAAAAAGGGCCAAGGAATTGAACTGGTTCTCCTCGAGGAGCTTATTCAGCAAATGGCAAATGTCCATTACACTGAGAATCTGACTGATGATCAACTGGATGCCATGGCAGGCAGTGAGACGCTGCGATATCAGGCAACTTCTTTCGGTGTTACTCGTAATAACAAGGCACTAATCAAGTCCACCAACAGGCTAAGGGACTCACTGCTACCAAAGGATGAATCCAAGTTGGCAATCCCCCTTCTAATACTTCTTGCTCAGCATCGTTCTGTGGTCATAATAGAGGCGAATGCACCTTACATCAAGATGGTTAGTGAACAGTTTGATAGATGTCATGGAACCCTCCTTCAATTTGTGGAATTTCTTTGCAGTGCTGTGACACCGGCATCGGCTTATGCTCAGCTAATTCCTTCGCTTGACGATCTTGTTCACAAATACCATCTTGATCCAGAGGTTGCTTTCTTAATTTATCGCCCTGTAATGAGGCTCTTCAAGTGCCAAGGGAGTTCTGATGTCTTCTGGCCTCTAGACAATATTGACACTCAAAGTATCACGTCTGCAAGTTCAGAGTCTGAAGCTACAGAGCATTCTGGAAATGTGGTTCTGGATCTTGGGTCTCCATGGAAGCCTATAACGTGGTCAGATCTTCTTAGTACTGCTGAAAGGATGCTGCCTTCAAAATCCTGGAATAGCCTGTCACCTGATCTCTATACTACATTTTGGGGGCTCACGCTCTATGATCTTTATGTCCCTAGAAATTGTTATGAGGCTGAAATTGCAAAGCAGCATGCTGCTCTTAAAGCTCTGGAAGAGATTTCTGATAATTCAAGTTCAGCAATAACCAAACGGAAGAAAGAAAAAGAACGAATTCAAGAATCCCTAGATCGCCTGACTAGCGAACTCCATAAGCATGAAGAAAACGTCGCATCTGTTCGTAAACGCTTATCTCGGGAGAAGGACAAATGGTTGAGTTCTTGCCCTGATACTTTGAAGATCAACATGGAGTTCCTTCAGCGTTGTATATTTCCACGCTGTACTTTCAGTATGCCAGATGCTGTGTATTGTGCCATGTTTGTGCACACTCTTCATTCCCTTGGGACACCATTTTTTAACACAGTCAACCACATTGATGTTCTCATCTGTAAAACCTTGCAGCCTATGATATGCTGCTGTACTGAATATGAAGTAGGTAGACTTGGGAAGTTTCTCTGTGAGACGTTGAGGATTGCTTACTATTGGAAGAGCGATGAATCTATTTATGAACGCGAATGTGGAAACATGCCAGGCTTTGCTGTATATTATAGATATCCAAATAGCCAGAGAGTTACCTACGGCCAGTTCATCAAGGTTCACTGGAAATGGAGTCAAAGGATTACAAAGTTATTGATTCAATGTCTGGAATCAACAGAGTACATGGAAATTCGAAATGCACTCATAATGTTGACAAAAATAACTAGTGTTTTTCCAGTTACTCGAAAAACTGGGATTAATCTTGAAAAACGGGTTTCCAAGATAAAGGGTGATGAGAGAGAGGACCTCAAGGTGCTGGCAACAGGTGTCTGTGCCTCTCTTGCTGCTAAAAAGCCTTCATGGGTTTCAGATGAGGAATTTGGTAACGGACATCTTGATTTAAAGAATGGACAAAACGGATCAAAGTCTTCTGCTGGTAATTCAATAGCTGCACAGGGTGAACCTATGGGAGGAAGAGTTGGAGCTGTACCCTCCCAACACTTGGATTCAATGAACTCAGGCAAAGAGCAGACTCTAAAAAGAAAACTTGTAGATGGGAGGTTGGAAAGAGGTGAAAGTAGCCCAACTGCAAAATCTGATCAAGGGCATGTAAAACTTAAAGCTAGCTCATCGGTGAATGGGTCAGATGCTAAGCCATCAATAACCTCAGCTGCTTTCCAACCTGCAACGGCAAGATCTATTGAAAATAAAAAACAAGTGAATGAATTCTCGAATAAAGCCTCAGATGAAAACATGGGGAAAGCTGCACCAAAGAACCCTACAGAATCTGAGTTGAGAGCTCAAGCAAAACGGTCAGTTCCTACTGGATCACTTGCCAAGGCGCTGAAACAAGATGTTGCAAAAGATGATAGTAGATCTGGAAAAGCAATTGGCAGAACTTCTAGTATCAGTGACAGAGATATTCTTTCCCATGCATCAGCTGGGACTTCAAATGTTTCCTCTTCTGCCGCAGTAAATGGTAACACAGATTCAGCGTCAGCTAAAGGTTCAGCTTCATTTGCGAGGACTGACATTCATGGTACTGAATCAAAGGTGGAGGTTGCAGCAGCAAAATCTTCTAATACAAGGATTTCTGCTCCAAAGGAAGATGGTGAAAATTCTGATGCACCAAGGCCCCATTCTTCTCGACTTGTTCACTCTCCTAGGCATGATAATTCTGCTGCTGCTTCAAAATCTAGTGATAAGCTGCAAAAGAGAACCAGTCCTGAAGAAACTGACAGACAGAGCAAACGGCGGAAAGGGACAACTGAAGTGAGAGATTTTGAGGGCGAAGCTCGAGTGTCAGACCGAGAGAGGTCTGTTGATGCTCGACTGTTGGATCCTGAAATTTCAGGTACTGATCATCAAAGTGTTCACAAGGCCACTGATAAGCCTTCTGACCGGTCCAAAGATAAGGGCAATGAAAGACATGAAAAGGATTATAGGGAAAGATTGGAGCGTCCTGAAAAGTCGCGTGGAGATGATTTGAATGAGAGATCCAGGGACAGGTCAATGGAGAGACATGGAAGGGAGCGTTCAGTAGAGAAACTTCAGGAGAGGGGTATGGATAGGAATTTGGATAGACTTCCTGACAAATCTAAGGATGACAGAGGTAAAGTAAGGTACAATGATGTATCAGCGGAAAAATCTCATGTTGATGATCGATATCATGGACAAAGCTTACCTCCGCCACCACCCTTACCTCCTCACATGCTTCCACATTCTGTTAGTTCTGCTAAAAGAGATGAAGATGAAGATAGAAGGTTTGGAACTACAAGACATACTCAGAGGCTTTCCCCAAGGCATGATGAGAAGGACCGTAGACGATCTGAAGATAATTCCTTAATTTCTCAAGATGATTCAAAACGTAGAAGAGAAGATGATTTTCGAGAAAGAAAGCGCGAAGATCGTGAGGGGCTGTCAATAAAGGTAGAGGAGCGGGAACGGGAGAGGGAGAGAGAGAGGGAGAAAGCAACCCTCTTGAAGGAGGATACAGATGCAATTGCGGCTTCTAAGAGGCGGAAACTTAAAAGAGAGCATCTGCCTTCCGGGGAAGCAGGTGAGTATTCACCAGTTCCCCCTCCACCTCCTCCTCTTTCAATTAGTTTGTCCCAGTCATATGATGGAAGGGATAGGGGAGACCGAAAAGGACCCATTGTCCAGCGATCAGGCTACTTGGAGGAACCAAGTCCGAGGATTCATGGTAAAGAAGTACCCAGCAAAATGGCTCGTCGTGAAACTGACCCGATGTATGAATGGGAGGATGAGAAAAGGCAAAGAGCTGAACAAAAGCGAAGGCATCGGAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000346 GO:0000347 GO:0000445 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006396 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0008104 GO:0008150 GO:0008152 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0015031 GO:0015833 GO:0015931 GO:0016070 GO:0016441 GO:0016458 GO:0016604 GO:0016607 GO:0019222 GO:0031047 GO:0031050 GO:0031503 GO:0031974 GO:0031981 GO:0032991 GO:0033036 GO:0034613 GO:0034641 GO:0035194 GO:0035195 GO:0035196 GO:0040029 GO:0042221 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048519 GO:0050657 GO:0050658 GO:0050789 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070727 GO:0070887 GO:0070918 GO:0071166 GO:0071310 GO:0071359 GO:0071407 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1990428

KEGG Pathways

Metabolic & Signaling

ko03013 ko03040 map03013 map03040

Pfam Domains

Protein Families

THOC2_N Tho2 Thoc2

Protein Analysis

1871

Amino Acids

211.86

Weight (kDa)

6.93

Isoelectric Point (pI)

46.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
THOC2_N	PF16134	37 - 409	2.3e-46	THO complex subunit 2 N-terminus
THOC2_N	PF16134	444 - 589	2.7e-19	THO complex subunit 2 N-terminus
Thoc2	PF11732	591 - 666	1.3e-29	Transcription- and export-related complex subunit
Tho2	PF11262	920 - 1215	1e-98	Transcription factor/nuclear export subunit protein 2

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0003911)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G24706 AT1G24706 AT1G24706 AT1G24706 AT1G24706 AT1G24706 AT1G24706 AT1G24706 AT1G24706
fragaria_vesca	FvH4_4g33200 FvH4_4g33200 FvH4_4g33200
malus_domestica	MD09G1258800.v1.1
prunus_persica	Prupe.3G150800_v2.0.a1 Prupe.3G150800_v2.0.a1 Prupe.3G150800_v2.0.a1 Prupe.3G150800_v2.0.a1 Prupe.3G150800_v2.0.a1 Prupe.3G150800_v2.0.a1 Prupe.3G150800_v2.0.a1 Prupe.3G150800_v2.0.a1 Prupe.3G150800_v2.0.a1
pyrus_communis	pycom09g17360
rosa_chinensis	RchiOBHm_Chr4g0442311
rosa_laevigata	RLG00000006018
rosa_multiflora	Rmu_sc0000124.1_g000055
rosa_roxburghii	Rroxscaffold_5G00383170
rosa_rugosa	Rorug04G0340300 Rorug04G0340400
rosa_samantha	Rh4AG391900 Rh4BG405400 Rh4CG420600 Rh4DG398700
rosa_wichuraiana	Rw4G033850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	572, 777
AarI	CACCTGC	1 cut(s)	5351
AbsI	CCTCGAGG	1 cut(s)	2108
Acc36I	ACCTGC	3 cut(s)	1576, 3994, 5351
Acc65I	GGTACC	2 cut(s)	1098, 1564
AccB1I	GGYRCC	2 cut(s)	1098, 1564
AccB7I	CCANNNNNTGG	3 cut(s)	1241, 1583, 3805
AccBSI	CCGCTC	3 cut(s)	1030, 1123, 5242
AccI	GTMKAC	3 cut(s)	1543, 3265, 5125
AccII	CGCG	3 cut(s)	3339, 4777, 5205
AcoI	YGGCCR	2 cut(s)	303, 3406
AcuI	CTGAAG	7 cut(s)	1412, 2667, 3068, 4551, 4593, 4838, 5154
AdeI	CACNNNGTG	1 cut(s)	3761
AfeI	AGCGCT	2 cut(s)	356, 740
AfiI	CCNNNNNNNGG	9 cut(s)	91, 1241, 1583, 2035, 2262, 3773, 3805, 4809, 5287
AflII	CTTAAG	1 cut(s)	1499
AflIII	ACRYGT	1 cut(s)	1538
AgeI	ACCGGT	1 cut(s)	4704
AhdI	GACNNNNNGTC	1 cut(s)	5459
AhlI	ACTAGT	2 cut(s)	1259, 3527
AjiI	CACGTC	1 cut(s)	2628
AjnI	CCWGG	5 cut(s)	271, 348, 2756, 3355, 4802
AjuI	GAANNNNNNNTTGG	2 cut(s)	4426, 4458
AloI	GAACNNNNNNTCC	4 cut(s)	2088, 2120, 2658, 2690
Alw21I	GWGCWC	5 cut(s)	123, 289, 1228, 3153, 4105
Alw26I	GTCTC	9 cut(s)	9, 258, 334, 699, 2186, 2694, 3281, 4816, 5436
Alw44I	GTGCAC	1 cut(s)	3149
AlwNI	CAGNNNCTG	4 cut(s)	2645, 3128, 4091, 4661
Ama87I	CYCGRG	3 cut(s)	2108, 3021, 4599
Aor51HI	AGCGCT	2 cut(s)	356, 740
AoxI	GGCC	8 cut(s)	171, 303, 1963, 2083, 2594, 3406, 4436, 4683
ApaLI	GTGCAC	1 cut(s)	3149
AseI	ATTAAT	2 cut(s)	581, 3561
AsiGI	ACCGGT	1 cut(s)	4704
Asp700I	GAANNNNTTC	5 cut(s)	1152, 1329, 2100, 2940, 4028
Asp718I	GGTACC	2 cut(s)	1098, 1564
AspA2I	CCTAGG	1 cut(s)	4466
AspLEI	GCGC	5 cut(s)	357, 741, 1426, 4147, 5205
AsuC2I	CCSGG	3 cut(s)	86, 92, 5353
AsuII	TTCGAA	1 cut(s)	3496
AsuNHI	GCTAGC	1 cut(s)	3926
AvaI	CYCGRG	3 cut(s)	2108, 3021, 4599
AvaII	GGWCC	8 cut(s)	88, 208, 601, 1352, 3607, 4707, 5119, 5453
AvrII	CCTAGG	1 cut(s)	4466
AxyI	CCTNAGG	1 cut(s)	1252
BaeGI	GKGCMC	1 cut(s)	3153
BalI	TGGCCA	1 cut(s)	305
BamHI	GGATCC	1 cut(s)	4642
BanI	GGYRCC	2 cut(s)	1098, 1564
BanII	GRGCYC	3 cut(s)	123, 2802, 4105
BarI	GAAGNNNNNNTAC	2 cut(s)	4836, 4868
BbsI	GAAGAC	3 cut(s)	437, 2581, 3723
Bbv12I	GWGCWC	5 cut(s)	123, 289, 1228, 3153, 4105
BbvCI	CCTCAGC	1 cut(s)	3970
BceAI	ACGGC	4 cut(s)	1748, 3421, 4008, 4571
BcgI	CGANNNNNNTGC	4 cut(s)	479, 513, 1745, 1779
BciT130I	CCWGG	5 cut(s)	273, 350, 2758, 3357, 4804
BciVI	GTATCC	1 cut(s)	5287
BclI	TGATCA	4 cut(s)	390, 2164, 3901, 4663
BcnI	CCSGG	3 cut(s)	86, 92, 5353
BcoDI	GTCTC	9 cut(s)	9, 258, 334, 699, 2186, 2694, 3281, 4816, 5436
BcuI	ACTAGT	2 cut(s)	1259, 3527
BfmI	CTRYAG	5 cut(s)	1035, 1382, 1393, 2652, 4083
BfoI	RGCGCY	3 cut(s)	358, 742, 4148
BfrI	CTTAAG	1 cut(s)	1499
BfuAI	ACCTGC	3 cut(s)	1576, 3994, 5351
BfuI	GTATCC	1 cut(s)	5287
BglII	AGATCT	4 cut(s)	1593, 2716, 3998, 4178
BlnI	CCTAGG	1 cut(s)	4466
BlpI	GCTNAGC	3 cut(s)	2331, 2472, 3954
BmcAI	AGTACT	1 cut(s)	2728
Bme1390I	CCNGG	8 cut(s)	86, 92, 273, 350, 2758, 3357, 4804, 5353
Bme18I	GGWCC	8 cut(s)	88, 208, 601, 1352, 3607, 4707, 5119, 5453
BmeRI	GACNNNNNGTC	1 cut(s)	5459
BmeT110I	CYCGRG	3 cut(s)	2108, 3021, 4599
BmgBI	CACGTC	1 cut(s)	2628
BmrFI	CCNGG	8 cut(s)	86, 92, 273, 350, 2758, 3357, 4804, 5353
BmrI	ACTGGG	3 cut(s)	948, 3564, 5410
BmtI	GCTAGC	1 cut(s)	3930
BmuI	ACTGGG	3 cut(s)	948, 3564, 5410
BpiI	GAAGAC	3 cut(s)	437, 2581, 3723
BplI	GAGNNNNNCTC	6 cut(s)	110, 142, 1112, 1144, 3594, 3626
BpmI	CTGGAG	2 cut(s)	294, 1910
Bpu10I	CCTNAGC	3 cut(s)	405, 2267, 3970
Bpu1102I	GCTNAGC	3 cut(s)	2331, 2472, 3954
Bpu14I	TTCGAA	1 cut(s)	3496
BpuEI	CTTGAG	5 cut(s)	240, 1917, 3596, 4089, 5139
BpuMI	CCSGG	3 cut(s)	86, 92, 5353
Bsa29I	ATCGAT	1 cut(s)	4966
BsaBI	GATNNNNATC	2 cut(s)	1464, 4968
BsaI	GGTCTC	2 cut(s)	2694, 5436
BsaWI	WCCGGW	1 cut(s)	4704
BsaXI	ACNNNNNCTCC	4 cut(s)	2088, 2118, 3765, 3795
Bsc4I	CCNNNNNNNGG	9 cut(s)	91, 1241, 1583, 2035, 2262, 3773, 3805, 4809, 5287
Bse118I	RCCGGY	2 cut(s)	2455, 4704
Bse21I	CCTNAGG	1 cut(s)	1252
Bse3DI	GCAATG	1 cut(s)	4729
Bse8I	GATNNNNATC	2 cut(s)	1464, 4968
BseBI	CCWGG	5 cut(s)	273, 350, 2758, 3357, 4804
BseCI	ATCGAT	1 cut(s)	4966
BseGI	GGATG	8 cut(s)	961, 1138, 1773, 2179, 2296, 2745, 4912, 5572
BseJI	GATNNNNATC	2 cut(s)	1464, 4968
BseLI	CCNNNNNNNGG	9 cut(s)	91, 1241, 1583, 2035, 2262, 3773, 3805, 4809, 5287
BseMI	GCAATG	1 cut(s)	4729
BseRI	GAGGAG	6 cut(s)	2096, 2126, 4998, 5252, 5382, 5385
BseSI	GKGCMC	1 cut(s)	3153
BseYI	CCCAGC	3 cut(s)	1759, 4244, 5519
BsgI	GTGCAG	3 cut(s)	1038, 3738, 4053
Bsh1236I	CGCG	3 cut(s)	3339, 4777, 5205
BshFI	GGCC	8 cut(s)	173, 305, 1965, 2085, 2596, 3408, 4438, 4685
BshNI	GGYRCC	2 cut(s)	1098, 1564
BshTI	ACCGGT	1 cut(s)	4704
BshVI	ATCGAT	1 cut(s)	4966
BsiHKAI	GWGCWC	5 cut(s)	123, 289, 1228, 3153, 4105
BsiHKCI	CYCGRG	3 cut(s)	2108, 3021, 4599
BsiSI	CCGG	5 cut(s)	86, 92, 2456, 4705, 5352
BsiWI	CGTACG	1 cut(s)	27
BslFI	GGGAC	7 cut(s)	2285, 2807, 3184, 4261, 4579, 4820, 5398
BslI	CCNNNNNNNGG	9 cut(s)	91, 1241, 1583, 2035, 2262, 3773, 3805, 4809, 5287
BsmAI	GTCTC	9 cut(s)	9, 258, 334, 699, 2186, 2694, 3281, 4816, 5436
BsmBI	CGTCTC	2 cut(s)	2186, 3281
BsmFI	GGGAC	7 cut(s)	2285, 2807, 3184, 4261, 4579, 4820, 5398
BsmI	GAATGC	4 cut(s)	273, 1291, 2368, 2659
BsnI	GGCC	8 cut(s)	173, 305, 1965, 2085, 2596, 3408, 4438, 4685
Bso31I	GGTCTC	2 cut(s)	2694, 5436
BsoBI	CYCGRG	3 cut(s)	2108, 3021, 4599
Bsp119I	TTCGAA	1 cut(s)	3496
Bsp1286I	GDGCHC	6 cut(s)	123, 289, 1228, 2802, 3153, 4105
Bsp1720I	GCTNAGC	3 cut(s)	2331, 2472, 3954
Bsp19I	CCATGG	4 cut(s)	132, 1297, 2178, 2693
BspANI	GGCC	8 cut(s)	173, 305, 1965, 2085, 2596, 3408, 4438, 4685
BspDI	ATCGAT	1 cut(s)	4966
BspFNI	CGCG	3 cut(s)	3339, 4777, 5205
BspMAI	CTGCAG	2 cut(s)	1039, 1386
BspMI	ACCTGC	3 cut(s)	1576, 3994, 5351
BspOI	GCTAGC	1 cut(s)	3930
BspQI	GCTCTTC	2 cut(s)	2567, 3308
BspT104I	TTCGAA	1 cut(s)	3496
BspT107I	GGYRCC	2 cut(s)	1098, 1564
BspTI	CTTAAG	1 cut(s)	1499
BspTNI	GGTCTC	2 cut(s)	2694, 5436
BsrBI	CCGCTC	3 cut(s)	1030, 1123, 5242
BsrDI	GCAATG	1 cut(s)	4729
BsrFI	RCCGGY	2 cut(s)	2455, 4704
BssAI	RCCGGY	2 cut(s)	2455, 4704
Bst2UI	CCWGG	5 cut(s)	273, 350, 2758, 3357, 4804
Bst4CI	ACNGT	8 cut(s)	1216, 1442, 1804, 2394, 3190, 4116, 4638, 5123
BstAFI	CTTAAG	1 cut(s)	1499
BstBI	TTCGAA	1 cut(s)	3496
BstC8I	GCNNGC	7 cut(s)	24, 1067, 1737, 2185, 2862, 3621, 3928
BstDSI	CCRYGG	4 cut(s)	132, 1297, 2178, 2693
BstENI	CCTNNNNNAGG	2 cut(s)	3771, 5285
BstF5I	GGATG	8 cut(s)	961, 1138, 1773, 2179, 2296, 2745, 4912, 5572
BstFNI	CGCG	3 cut(s)	3339, 4777, 5205
BstH2I	RGCGCY	3 cut(s)	358, 742, 4148
BstHHI	GCGC	5 cut(s)	357, 741, 1426, 4147, 5205
BstMAI	GTCTC	9 cut(s)	9, 258, 334, 699, 2186, 2694, 3281, 4816, 5436
BstMWI	GCNNNNNNNGC	9 cut(s)	1577, 1781, 2122, 2849, 2873, 2967, 3644, 4322, 5309
BstNI	CCWGG	5 cut(s)	273, 350, 2758, 3357, 4804
BstNSI	RCATGY	5 cut(s)	1542, 2864, 3355, 3914, 5017
BstSCI	CCNGG	8 cut(s)	84, 90, 271, 348, 2756, 3355, 4802, 5351
BstSFI	CTRYAG	5 cut(s)	1035, 1382, 1393, 2652, 4083
BstSLI	GKGCMC	1 cut(s)	3153
BstUI	CGCG	3 cut(s)	3339, 4777, 5205
BstV2I	GAAGAC	3 cut(s)	437, 2581, 3723
BstX2I	RGATCY	9 cut(s)	946, 1459, 1593, 2680, 2716, 3998, 4178, 4642, 4799
BstXI	CCANNNNNNTGG	2 cut(s)	313, 5527
BstYI	RGATCY	9 cut(s)	946, 1459, 1593, 2680, 2716, 3998, 4178, 4642, 4799
Bsu15I	ATCGAT	1 cut(s)	4966
Bsu36I	CCTNAGG	1 cut(s)	1252
BsuI	GTATCC	1 cut(s)	5287
BsuRI	GGCC	8 cut(s)	173, 305, 1965, 2085, 2596, 3408, 4438, 4685
BsuTUI	ATCGAT	1 cut(s)	4966
BtgI	CCRYGG	4 cut(s)	132, 1297, 2178, 2693
BtgZI	GCGATG	1 cut(s)	3333
BtrI	CACGTC	1 cut(s)	2628
BtsCI	GGATG	8 cut(s)	961, 1138, 1773, 2179, 2296, 2745, 4912, 5572
BtsI	GCAGTG	3 cut(s)	2194, 2276, 2449
BtsIMutI	CAGTG	9 cut(s)	1212, 1447, 2145, 2194, 2276, 2449, 3424, 4219, 4686
BveI	ACCTGC	3 cut(s)	1576, 3994, 5351
Cac8I	GCNNGC	7 cut(s)	24, 1067, 1737, 2185, 2862, 3621, 3928
CaiI	CAGNNNCTG	4 cut(s)	2645, 3128, 4091, 4661
CfoI	GCGC	5 cut(s)	357, 741, 1426, 4147, 5205
Cfr10I	RCCGGY	2 cut(s)	2455, 4704
ClaI	ATCGAT	1 cut(s)	4966
CseI	GACGC	4 cut(s)	1913, 2203, 4287, 4751
CspAI	ACCGGT	1 cut(s)	4704
DraI	TTTAAA	1 cut(s)	3705
DraIII	CACNNNGTG	1 cut(s)	3761
DriI	GACNNNNNGTC	1 cut(s)	5459
EaeI	YGGCCR	2 cut(s)	303, 3406
Eam1105I	GACNNNNNGTC	1 cut(s)	5459
EciI	GGCGGA	3 cut(s)	4573, 4980, 5338
Ecl136II	GAGCTC	2 cut(s)	121, 4103
Eco24I	GRGCYC	3 cut(s)	123, 2802, 4105
Eco31I	GGTCTC	2 cut(s)	2694, 5436
Eco32I	GATATC	4 cut(s)	238, 2204, 3380, 4970
Eco47I	GGWCC	8 cut(s)	88, 208, 601, 1352, 3607, 4707, 5119, 5453
Eco47III	AGCGCT	2 cut(s)	356, 740
Eco53kI	GAGCTC	2 cut(s)	121, 4103
Eco57I	CTGAAG	7 cut(s)	1412, 2667, 3068, 4551, 4593, 4838, 5154
Eco81I	CCTNAGG	1 cut(s)	1252
Eco88I	CYCGRG	3 cut(s)	2108, 3021, 4599
EcoICRI	GAGCTC	2 cut(s)	121, 4103
EcoNI	CCTNNNNNAGG	2 cut(s)	3771, 5285
EcoO109I	RGGNCCY	3 cut(s)	3607, 4437, 5453
EcoRI	GAATTC	2 cut(s)	2940, 4028
EcoRII	CCWGG	5 cut(s)	271, 348, 2756, 3355, 4802
EcoRV	GATATC	4 cut(s)	238, 2204, 3380, 4970
EcoT22I	ATGCAT	3 cut(s)	1291, 1870, 4240
EcoT38I	GRGCYC	3 cut(s)	123, 2802, 4105
Esp3I	CGTCTC	2 cut(s)	2186, 3281
FaqI	GGGAC	7 cut(s)	2285, 2807, 3184, 4261, 4579, 4820, 5398
FauI	CCCGC	1 cut(s)	5235
FauNDI	CATATG	1 cut(s)	5422
FbaI	TGATCA	4 cut(s)	390, 2164, 3901, 4663
FblI	GTMKAC	3 cut(s)	1543, 3265, 5125
FokI	GGATG	8 cut(s)	968, 1125, 1780, 2186, 2303, 2752, 4919, 5579
FriOI	GRGCYC	3 cut(s)	123, 2802, 4105
GlaI	GCGC	5 cut(s)	356, 740, 1425, 4146, 5204
GsaI	CCCAGC	3 cut(s)	1763, 4248, 5523
GsuI	CTGGAG	2 cut(s)	294, 1910
HaeII	RGCGCY	3 cut(s)	358, 742, 4148
HaeIII	GGCC	8 cut(s)	173, 305, 1965, 2085, 2596, 3408, 4438, 4685
HapII	CCGG	5 cut(s)	86, 92, 2456, 4705, 5352
HgaI	GACGC	4 cut(s)	1913, 2203, 4287, 4751
HhaI	GCGC	5 cut(s)	357, 741, 1426, 4147, 5205
Hin6I	GCGC	5 cut(s)	355, 739, 1424, 4145, 5203
HinP1I	GCGC	5 cut(s)	355, 739, 1424, 4145, 5203
HincII	GTYRAC	2 cut(s)	3193, 3516
HindII	GTYRAC	2 cut(s)	3193, 3516
HindIII	AAGCTT	2 cut(s)	1527, 4981
HpaII	CCGG	5 cut(s)	86, 92, 2456, 4705, 5352
Hpy99I	CGWCG	4 cut(s)	200, 1224, 2999, 5538
HpyCH4III	ACNGT	8 cut(s)	1216, 1442, 1804, 2394, 3190, 4116, 4638, 5123
HpyCH4IV	ACGT	7 cut(s)	30, 1899, 2627, 2708, 2994, 3290, 5170
HpyF10VI	GCNNNNNNNGC	9 cut(s)	1577, 1781, 2122, 2849, 2873, 2967, 3644, 4322, 5309
HpySE526I	ACGT	7 cut(s)	30, 1899, 2627, 2708, 2994, 3290, 5170
HspAI	GCGC	5 cut(s)	355, 739, 1424, 4145, 5203
KpnI	GGTACC	2 cut(s)	1102, 1568
Ksp22I	TGATCA	4 cut(s)	390, 2164, 3901, 4663
LguI	GCTCTTC	2 cut(s)	2567, 3308
MaeII	ACGT	7 cut(s)	30, 1899, 2627, 2708, 2994, 3290, 5170
MbiI	CCGCTC	3 cut(s)	1030, 1123, 5242
MfeI	CAATTG	5 cut(s)	248, 1559, 1815, 4191, 5304
MflI	RGATCY	9 cut(s)	946, 1459, 1593, 2680, 2716, 3998, 4178, 4642, 4799
MhlI	GDGCHC	6 cut(s)	123, 289, 1228, 2802, 3153, 4105
MlsI	TGGCCA	1 cut(s)	305
MluNI	TGGCCA	1 cut(s)	305
MlyI	GAGTC	6 cut(s)	1461, 1876, 2267, 2651, 3445, 3829
MmeI	TCCRAC	8 cut(s)	219, 1564, 1689, 1745, 3763, 3846, 4006, 4620
Mox20I	TGGCCA	1 cut(s)	305
Mph1103I	ATGCAT	3 cut(s)	1291, 1870, 4240
MroXI	GAANNNNTTC	5 cut(s)	1152, 1329, 2100, 2940, 4028
MscI	TGGCCA	1 cut(s)	305
MslI	CAYNNNNRTG	7 cut(s)	873, 2378, 2982, 3146, 3201, 4671, 4959
Msp20I	TGGCCA	1 cut(s)	305
MspA1I	CMGCKG	6 cut(s)	1580, 1759, 1888, 3974, 4244, 4943
MspCI	CTTAAG	1 cut(s)	1499
MspI	CCGG	5 cut(s)	86, 92, 2456, 4705, 5352
MspR9I	CCNGG	8 cut(s)	86, 92, 273, 350, 2758, 3357, 4804, 5353
MunI	CAATTG	5 cut(s)	248, 1559, 1815, 4191, 5304
Mva1269I	GAATGC	4 cut(s)	273, 1291, 2368, 2659
MvaI	CCWGG	5 cut(s)	273, 350, 2758, 3357, 4804
MvnI	CGCG	3 cut(s)	3339, 4777, 5205
MwoI	GCNNNNNNNGC	9 cut(s)	1577, 1781, 2122, 2849, 2873, 2967, 3644, 4322, 5309
NciI	CCSGG	3 cut(s)	86, 92, 5353
NcoI	CCATGG	4 cut(s)	132, 1297, 2178, 2693
NdeI	CATATG	1 cut(s)	5422
NheI	GCTAGC	1 cut(s)	3926
NmuCI	GTSAC	7 cut(s)	31, 976, 1943, 2009, 2449, 2769, 4214
NsiI	ATGCAT	3 cut(s)	1291, 1870, 4240
NspI	RCATGY	5 cut(s)	1542, 2864, 3355, 3914, 5017
NspV	TTCGAA	1 cut(s)	3496
PaeI	GCATGC	1 cut(s)	2864
PaeR7I	CTCGAG	2 cut(s)	2108, 4599
PaqCI	CACCTGC	1 cut(s)	5351
PciI	ACATGT	1 cut(s)	1538
PciSI	GCTCTTC	2 cut(s)	2567, 3308
PctI	GAATGC	4 cut(s)	273, 1291, 2368, 2659
PdmI	GAANNNNTTC	5 cut(s)	1152, 1329, 2100, 2940, 4028
Pfl23II	CGTACG	1 cut(s)	27
PflMI	CCANNNNNTGG	3 cut(s)	1241, 1583, 3805
PfoI	TCCNGGA	3 cut(s)	84, 348, 2756
PinAI	ACCGGT	1 cut(s)	4704
PleI	GAGTC	6 cut(s)	1460, 1876, 2267, 2650, 3444, 3829
PpsI	GAGTC	6 cut(s)	1460, 1876, 2267, 2650, 3444, 3829
PpuMI	RGGWCCY	2 cut(s)	3607, 5453
PscI	ACATGT	1 cut(s)	1538
PshBI	ATTAAT	2 cut(s)	581, 3561
PsiI	TTATAA	2 cut(s)	572, 777
Psp124BI	GAGCTC	2 cut(s)	123, 4105
Psp5II	RGGWCCY	2 cut(s)	3607, 5453
Psp6I	CCWGG	5 cut(s)	271, 348, 2756, 3355, 4802
PspFI	CCCAGC	3 cut(s)	1759, 4244, 5519
PspGI	CCWGG	5 cut(s)	271, 348, 2756, 3355, 4802
PspLI	CGTACG	1 cut(s)	27
PspPPI	RGGWCCY	2 cut(s)	3607, 5453
PspXI	VCTCGAGB	2 cut(s)	2108, 4599
PstI	CTGCAG	2 cut(s)	1039, 1386
PstNI	CAGNNNCTG	4 cut(s)	2645, 3128, 4091, 4661
PsuI	RGATCY	9 cut(s)	946, 1459, 1593, 2680, 2716, 3998, 4178, 4642, 4799
PvuII	CAGCTG	5 cut(s)	1580, 1759, 1888, 3974, 4244
RseI	CAYNNNNRTG	7 cut(s)	873, 2378, 2982, 3146, 3201, 4671, 4959
SacI	GAGCTC	2 cut(s)	123, 4105
SapI	GCTCTTC	2 cut(s)	2567, 3308
ScaI	AGTACT	1 cut(s)	2728
SchI	GAGTC	6 cut(s)	1461, 1876, 2267, 2651, 3445, 3829
ScrFI	CCNGG	8 cut(s)	86, 92, 273, 350, 2758, 3357, 4804, 5353
SduI	GDGCHC	6 cut(s)	123, 289, 1228, 2802, 3153, 4105
SfcI	CTRYAG	5 cut(s)	1035, 1382, 1393, 2652, 4083
Sfr274I	CTCGAG	2 cut(s)	2108, 4599
SfuI	TTCGAA	1 cut(s)	3496
SinI	GGWCC	8 cut(s)	88, 208, 601, 1352, 3607, 4707, 5119, 5453
SlaI	CTCGAG	2 cut(s)	2108, 4599
SmiMI	CAYNNNNRTG	7 cut(s)	873, 2378, 2982, 3146, 3201, 4671, 4959
SmlI	CTYRAG	8 cut(s)	255, 1499, 1932, 2108, 3611, 4104, 4599, 5154
SmoI	CTYRAG	8 cut(s)	255, 1499, 1932, 2108, 3611, 4104, 4599, 5154
SpeI	ACTAGT	2 cut(s)	1259, 3527
SphI	GCATGC	1 cut(s)	2864
SspI	AATATT	2 cut(s)	1239, 2608
SstI	GAGCTC	2 cut(s)	123, 4105
StyD4I	CCNGG	8 cut(s)	84, 90, 271, 348, 2756, 3355, 4802, 5351
TaaI	ACNGT	8 cut(s)	1216, 1442, 1804, 2394, 3190, 4116, 4638, 5123
TaiI	ACGT	7 cut(s)	33, 1902, 2630, 2711, 2997, 3293, 5173
TaqII	GACCGA	2 cut(s)	4626, 5460
TatI	WGTACW	5 cut(s)	1804, 2726, 3107, 3245, 3483
TauI	GCSGC	6 cut(s)	154, 173, 998, 1126, 4274, 5312
TscAI	CASTG	9 cut(s)	1219, 1447, 2152, 2194, 2283, 2449, 3431, 4219, 4693
TseFI	GTSAC	7 cut(s)	31, 976, 1943, 2009, 2449, 2769, 4214
Tsp45I	GTSAC	7 cut(s)	31, 976, 1943, 2009, 2449, 2769, 4214
TspGWI	ACGGA	5 cut(s)	324, 1075, 2935, 3705, 3735
TspRI	CASTG	9 cut(s)	1219, 1447, 2152, 2194, 2283, 2449, 3431, 4219, 4693
Van91I	CCANNNNNTGG	3 cut(s)	1241, 1583, 3805
Vha464I	CTTAAG	1 cut(s)	1499
VneI	GTGCAC	1 cut(s)	3149
VpaK11BI	GGWCC	8 cut(s)	88, 208, 601, 1352, 3607, 4707, 5119, 5453
VspI	ATTAAT	2 cut(s)	581, 3561
XagI	CCTNNNNNAGG	2 cut(s)	3771, 5285
XbaI	TCTAGA	1 cut(s)	2599
XceI	RCATGY	5 cut(s)	1542, 2864, 3355, 3914, 5017
XcmI	CCANNNNNNNNNTGG	3 cut(s)	313, 4241, 5423
XhoI	CTCGAG	2 cut(s)	2108, 4599
XmaJI	CCTAGG	1 cut(s)	4466
XmiI	GTMKAC	3 cut(s)	1543, 3265, 5125
XmnI	GAANNNNTTC	5 cut(s)	1152, 1329, 2100, 2940, 4028
ZrmI	AGTACT	1 cut(s)	2728
Zsp2I	ATGCAT	3 cut(s)	1291, 1870, 4240

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.