Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0003911

Physical Location & Seq

Genomic Coordinates Forward (+)

31499316 .. 31512816

Gene Structure 13501 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g33200.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5583 bp

ATGTCTCTCCCGCCCGTAGAGCGTGCACACATCAACGAGGATCACCTCCGTGAATGGAAGACCGGAAACCCTAGCTTCAAGCTACCGGAGCCTGTTCCGATGCTCCGGTTCCTCTACGAGCTCTGCTCCACCATGGTTCGTGGAGAATTGCCGGTTCAGAAGTGTAGAGCGGCTCTGGATTCGGTGGAGTTTTCTGAAAAGGTTTCCGAGCAAGAGCTGGCTTCAAGCTTGGCTGATATTGTTACTCAAATGTCTCAAGATCTTACCATGCCTGGAGAGCATCGAGCTCGTCTCACAAAGCTGGCCAAATGGTTGGTGGAATCTTCTTTAGTTCCATTAAGGCTTTTTCAGGAGCGCTGTGAGGAAGAATTTTTGTGGGAGGCTGAAATGATTAAGATAAAGGCTCAAGAACTGAAGAGTAAAGAGGTTAGAGTAAACACCCGTCTTCTATATCAGCAAACAAAGTTTAATCTTCTTCGAGAAGAGAGCGAGGGATATGCCAAGCTGGTTACACTTCTTTGCCAAAATTCAGAAAATTCATCGCACAATGCTGGAGCAACAATAGGCATTATAAAGTCATTAATCGGACATTTTGATCTGGACCCAAATCATGTTTTTGATATCGTTTTGGAGTGCTTCGAACTTCTGCCAGACAACAATGTCTTTTTGGAGTTGATACCAATTTTTCCAAAGTCTCATGCTTCACAAATCCTTGGTTTTAAATTTCAACACTACCAGCGTTTGGAAGTAAATGATCCTGTACCATTTGGACTTTATAAGCTTACAGCTTTGCTGGTGAAGGAGAATTTTATTGATCTTGATAGCATATGTGCTCATCTACTTCCGAAGGACGATGAGGCATTTGAGCATTATAGCTCTTTCTCCTCCAAGCAACTTGATGAGGCTAATAAAATTGGTAAAATAAATCTTGCTGCTACTGGGAAGGATCTAATGGAAGATGAGAAACAAGGGGACGTGACAATAGATCTCTTTGCATCTTTAGACATGGATTCAGTGGCAGTAGGAGAACGATCTACAGAGTTTGAAAATAATCAAACTTTGGGATTGCTTACTGGTTTTCTTGCGGTAGATGACTGGTATCATGCGAATTTACTATTTGACCGTCTGTCACCTCTAAATCCTGTTGAACATACCCAAATATGTAATAGCTTGTTTAGACTCATTGAAAAATCAATCTCTTCTGCATACGACATGGTTCATCAAGCACGTCTCAATAACTTGGGCTCCTCAGGAACCGGTGTTGGTGTGATGACTACAGAAAACTCATCAGCTAGTGGATCTTTTATTGAACTTCAAAAAGAACTTTTTCAGATGCTTGCTATTGTTGGGCCTTACCTTTATCGTGATACTTTGCTGCTACAGAAGGTTTGCAGAGTATTAAAAGGTTATTACTTGTCTGCACCGGAGCTTGGAATACCTGGTGAAGTAGCTGTTTCTGCAAGTAATCCTGGTTTGCCCCTTAAGGAAGCTAGGTCCCCCCGTCAGGTAGCAATGTCAAGGGTAGAGGAAGCCTTCCGGACATGTCTACTTCCATCTTTACAGTTAGTACCTGCAAATCCAGCTGTTGGAATGGAGATTTGGGAAGTGATGAGTCTACTTCCATATGAGGTGAGGTACCGTTTATACGGTGAATGGGAGAAAGAAGATGAACGAATTCCTATAGTTTTAGCTGCAAGGCAAACAGCCAAGTTGGACACTAGAAGAATTTTGAAGCGGCTTGCAAAAGAAAATTTAAAGCAGCAGAGTCGGATGGTTGCAAAGTTAGCTCATGCAAATCCAATGACTGTACTTCGAACCATTGTTCATCAGATTGAGGCATACAAAGATATGATTACTCCTGTTGTAGATGCATTTAAGTATTTGACTCAGCTGGAGTATGATGTCCTGGAATATGTTGTGATTGAACGTCTGGCGCAAGGAGGGCGTGATAAGTTAAAAGAGGATGGGCTTAATTTGTCAGACTGGCTTCAATCTTTGGCTTCATTTTGGGGTCACTTGTGTAAAAAATACCCATCTATGGAATTGAGAAGCCTTTTCCAGTATCTTGTAAATCAGTTGAAAAAGGGGCAAGGAATTGAGCTTGTTGTTCTTCAGGAGCTTATTCAGCAAATGGCAAATGTCCATCACACGGAGGACCTTACTGAAGATCAGCTGGATGCCATGGCTGGGGGTGAGACTCTTCGTCATCTGACCACTGGTTTTGGTGTTACTCGACATAACAAGCAATTAATCAAGTCCACAAACAGGCTAAGGGAGTCGCTGCTTCCAAAAGATGAAACAAAGCTTGCAATCCCTCTGTTGTTGCATCTTGCTCAGCATCGTTCTTTGGTCATAATAGATGCAGATGCGCCTTATATTAAGATGGTTTGCGAGCAGTTTGATAGATGTCACGGAGCACTCCTTCAGTATGTGGAATTTCTTTGCAGTGCCATGCCATCGGCATCAGCTTATGCTCAGCTAATTCCTTCACTTGACGATCTTGTCCACAAATACCACCTTGAGCCTGAGGTTGCTTTCTTAATTTATCGCCCAGTAATGAGGCTGTTCAAGACCCCACCAAGTTCTGATGTCTTCTGGCCTCTTGACAATAATGATGCTCAAAGTATCACATCTGCAATTTCAGAGTCTGAAGCTGCACAGAATTCTGGCAACGTGGTTCTGGATCTTGGGTCTACATGGAACCCTATAACGTGGTTGGATCTTCTTGATACTGCTAAAACCATGTTGCCTGCAAGAGCCTGGAATAGTTTGTCCCCTGATCTCTATGCTACATTTTGGGGGCTCACACTCTATGATCTTTATGTCCCTAGAAATTGTTACATATCTGAAATTGCGAAACAGCAGGCTTCTATCAAAGCTCTGGAAGAGCAGCCTGATAATTCAATTTCTGAAATATTAAGAAGGAAGAAGGAGAAGGAAAGAATTCAAGAAACAATAGATCGGCTGATTAGTGAATCCCGTAAACACGAGGAACATGTTGCATCTGTTCGTAAACGGTTATTGCGTGAGAAGGACAAATGGTTGAGTTCGTGTCCTGATACTTTAAAGATCAACATGGAATTTCTTCAGCGTTGTATATTTCCTCGCTGTACTTTCAGCATGCCAGATGCTGTGTATTCTGCCATGTTTGTCCACACTCTTCATACCCTGGGGACACCATTTTTCAACACTGTCAACCACATGGATGTGCTTATCTGTAGAACCTTACAACCTATGATATGCTGCTGCACTGAATCTGAAGTTGGTAGATTGGGGAAGTTTCTCTGTGAGACCTTGAAGATTGCTTACTATTGGAAGAGTGATGAATCTATTTATGAGCGTGAGTGCGGAAACATGCCGGGCTTTGCTGTCTATTATAGATTTCCAGACAGCCAGCGGGTCCGATATGGCCAGTTTGTCAAGGTGCACTGGAAATGGAGTCAAAGGATCACAAGATTATTGGGTCAGTGTCTGGAATCTACGGAGTACATGGAAATTCGAAATGCTCTCATAATCTTATCAAGAATTTCTAGTGTTTTTCCAGTTACTAGAAAGAGTGCGTTGAACCTCGAAAAACGGGTCTCCAAGATCAAAGGTGATGGGAGAGAGGACCTCAAGGTGTTGGCAACAAGTGTTGGTGCTTCTTTGGCTGCTAGAAAGCCTTCTTTGGTTTCGGATGAAGAATTTTGTATGGGATACGTTGAACTAAAGTCTGCATCATCTTCAAAGCCTTTGGCCAGTAATTCAGGAGCTATACATAGCGGTCCTGCCGTTAATAATTCTCAAACTGAACCTGCTGGCGGAAAAGCAGGAACTCTTGTCTCCCAACACGCTGAATTAATAGATTCAGCCAGAGACCACGTGTCAAAAGCTAAACCTGCAGATGGGAGGTCGGAAAGAGCAGAAAGTGTCTCAACTGCAAAATCTGATCCAGGGCATCTAAAACATAAAGGTGCCTCGTTGGTGAATGGCTCAGATGCTCAGGCATCTGTACCATCAGCTACTTTACAAGCTGGAACAGCAAGGCCTATCGAAAATCAAGTACAACTGAACGAAACCTCAACTAGAAGGGCGGAAGAAAATACGGGAAAACTTGCCGCAAAGAACACTTCAGAATCTGAGTTGAGGGCCCAAGCAAAACGGTCTGTTCCTGCTGGGGCTAAGCCATTAAAACAAGACCTTGTGAAGGACGAAAGTAGATCTGGAAAAGCAGCTGGGGCTACTAATGTCTCTTCCATCACAGCAAATGGTAGCACGGTACCATCTTTGGGGAAAGGTTCAGCTTCATTAGGTATTGAATCAAAAGTGGAGGCTGGATCTGCAAAGATTTCCAATACAAGGATTCCTTCTTCAAAGGAAGAAGGTGCTGAGGTTTCTGATGTGGCAAGGCCCCCTTCTTCTCGATTCGTTAATTCTCCTAGACATGATAGTTCTGCTACACTCTCCAAGTCCAGTGATAAACTTCAAAAAAGAACCGGCCCTGCAGAAGAAACAGATAGACAAAGTAAACGCCGGAAAGGGGAGGCTGAAATGAGAGATTCTGAGGGTGAAGCTCGGTTGTCTGACCGAGAGAGGTCCGTTGATGCTAGACTGTTGGATCTAGACAAATCAGGAAGCGATGATAGAAGTGTTTATAAGGCCACTGAGAAGGCTTCAGATCGGTCTAAAGATAAAGGAAATGAAAGACATGATAAGGATCACAGGGAAAGAGCGGACCGTCCTGATAAGTCACGCGGAGATGATTTAGTTGAGAGATCCAGAGATAGGTCAATGGAGAGACATGGAAGGGATCATTCAGCTGAGAAATTGCAAGAGAGGGGTTCAGATAGGAGTTTCGATAGACTGCCTGAAAAATCAAAGGATGAGAAAGGTAAAGGGCGTTACAGTGATATATCCACTGAAAAATCTCATGTTGATGAACGATATCACGGACAAAGCTTACCTCCACCACCACCCTTACCTCCTCACATTGTTCCTCAATCTGTTAGTTCCGGAAGAAGAGATGAAGACTCAGATAGAAGGACAACTACAAGGCATACTCAAAGGCTTTCTCCGAGACATGATGAGAAGGAACGTAGAAGATCTGAAGAAAATTCCTCAATTTCTCAAGATGATTCGAAGCGTAGAAGAGAAGATGATTTTCGAGAGAGAAAACGTGATGACCGTGAGGGGATCTCAGTAAAGGTGGATGAGAGGGATAGGGACAGGGACAGGGACAGGGAAAGGGAGCGGGAGAAAGAAAGGGAGAAAGCCAACCTATCGAAGGAGGATCCAGACATGATTGCAGCTTCTAAGAGGCGGAAACTTAAACGAGATCTGTCTTCTGTGGAAGCTGGTGAGTACTCACCAGTTCACCCTCCCCCTCCCCTCTCTATTAACTTGTCCCAGTCATACGATGGAAGGGATAGGGGAGAACGGAAAGGACCCATTGTCGCACGCACGGGTTACGTAGAGGAACCAAGTTTGAGGATTCATGGTAAAGAAGTATCCAACAAGATGACTCGCCGTGATACAGACCCGATGTACGAATGGGATGACGATAAAAGGCGAGGTGAACAAAAACGAAGGCATCGGAAGAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000346 GO:0000347 GO:0000445 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006396 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0008104 GO:0008150 GO:0008152 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0015031 GO:0015833 GO:0015931 GO:0016070 GO:0016441 GO:0016458 GO:0016604 GO:0016607 GO:0019222 GO:0031047 GO:0031050 GO:0031503 GO:0031974 GO:0031981 GO:0032991 GO:0033036 GO:0034613 GO:0034641 GO:0035194 GO:0035195 GO:0035196 GO:0040029 GO:0042221 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048519 GO:0050657 GO:0050658 GO:0050789 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070727 GO:0070887 GO:0070918 GO:0071166 GO:0071310 GO:0071359 GO:0071407 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1990428

KEGG Pathways

Metabolic & Signaling

ko03013 ko03040 map03013 map03040

Pfam Domains

Protein Families

THOC2_N Tho2 Thoc2

Protein Analysis

1861

Amino Acids

210.24

Weight (kDa)

6.99

Isoelectric Point (pI)

48.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
THOC2_N	PF16134	37 - 412	6.9e-45	THO complex subunit 2 N-terminus
THOC2_N	PF16134	439 - 590	1.5e-21	THO complex subunit 2 N-terminus
Thoc2	PF11732	592 - 667	6.8e-30	Transcription- and export-related complex subunit
Tho2	PF11262	921 - 1215	1.5e-93	Transcription factor/nuclear export subunit protein 2

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0003911)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G24706 AT1G24706 AT1G24706 AT1G24706 AT1G24706 AT1G24706 AT1G24706 AT1G24706 AT1G24706
fragaria_vesca	FvH4_4g33200 FvH4_4g33200 FvH4_4g33200
malus_domestica	MD09G1258800.v1.1
prunus_persica	Prupe.3G150800_v2.0.a1 Prupe.3G150800_v2.0.a1 Prupe.3G150800_v2.0.a1 Prupe.3G150800_v2.0.a1 Prupe.3G150800_v2.0.a1 Prupe.3G150800_v2.0.a1 Prupe.3G150800_v2.0.a1 Prupe.3G150800_v2.0.a1 Prupe.3G150800_v2.0.a1
pyrus_communis	pycom09g17360
rosa_chinensis	RchiOBHm_Chr4g0442311
rosa_laevigata	RLG00000006018
rosa_multiflora	Rmu_sc0000124.1_g000055
rosa_roxburghii	Rroxscaffold_5G00383170
rosa_rugosa	Rorug04G0340300 Rorug04G0340400
rosa_samantha	Rh4AG391900 Rh4BG405400 Rh4CG420600 Rh4DG398700
rosa_wichuraiana	Rw4G033850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	572, 777, 4635
AasI	GACNNNNNNGTC	2 cut(s)	659, 2501
Acc36I	ACCTGC	3 cut(s)	1579, 3802, 3886
Acc65I	GGTACC	2 cut(s)	1635, 4258
AccB1I	GGYRCC	3 cut(s)	1635, 3953, 4258
AccB7I	CCANNNNNTGG	2 cut(s)	742, 1586
AccBSI	CCGCTC	3 cut(s)	170, 4712, 5230
AccI	GTMKAC	3 cut(s)	1546, 1615, 2693
AccII	CGCG	1 cut(s)	4734
AccIII	TCCGGA	2 cut(s)	1536, 4991
AcoI	YGGCCR	3 cut(s)	303, 3409, 3735
AcvI	CACGTG	1 cut(s)	3862
AfeI	AGCGCT	1 cut(s)	356
AflII	CTTAAG	1 cut(s)	1481
AflIII	ACRYGT	3 cut(s)	1541, 2995, 3861
AgeI	ACCGGT	1 cut(s)	1256
AhdI	GACNNNNNGTC	4 cut(s)	1126, 2202, 3862, 5429
AjiI	CACGTC	2 cut(s)	976, 1229
AjnI	CCWGG	7 cut(s)	271, 1438, 1468, 1905, 2759, 3168, 3931
AjuI	GAANNNNNNNTTGG	4 cut(s)	1582, 1614, 3246, 3278
AleI	CACNNNNGTG	1 cut(s)	48
Alw21I	GWGCWC	6 cut(s)	28, 123, 289, 835, 2419, 3429
Alw44I	GTGCAC	2 cut(s)	24, 3425
AlwNI	CAGNNNCTG	4 cut(s)	2648, 3131, 3472, 4118
Aor13HI	TCCGGA	2 cut(s)	1536, 4991
Aor51HI	AGCGCT	1 cut(s)	356
ApaI	GGGCCC	1 cut(s)	4132
ApaLI	GTGCAC	2 cut(s)	24, 3425
ArsI	GACNNNNNNTTYG	2 cut(s)	3564, 3596
AseI	ATTAAT	3 cut(s)	581, 2249, 3839
AsiGI	ACCGGT	1 cut(s)	1256
Asp700I	GAANNNNTTC	5 cut(s)	1326, 1674, 2943, 3084, 3661
Asp718I	GGTACC	2 cut(s)	1635, 4258
AspLEI	GCGC	3 cut(s)	357, 1936, 2371
AsuC2I	CCSGG	1 cut(s)	3360
AsuII	TTCGAA	4 cut(s)	639, 1813, 3499, 5117
AvaII	GGWCC	9 cut(s)	601, 1494, 2155, 3400, 3610, 3764, 4575, 4714, 5423
AxyI	CCTNAGG	2 cut(s)	1249, 2526
BaeGI	GKGCMC	3 cut(s)	28, 3429, 4132
BalI	TGGCCA	3 cut(s)	305, 3411, 3737
BamHI	GGATCC	1 cut(s)	5269
BanI	GGYRCC	3 cut(s)	1635, 3953, 4258
BanII	GRGCYC	5 cut(s)	123, 289, 1247, 2805, 4132
BarI	GAAGNNNNNNTAC	4 cut(s)	3290, 3322, 5011, 5043
BauI	CACGAG	1 cut(s)	2987
BbrPI	CACGTG	1 cut(s)	3862
BbsI	GAAGAC	5 cut(s)	65, 437, 2584, 5013, 5313
Bbv12I	GWGCWC	6 cut(s)	28, 123, 289, 835, 2419, 3429
BbvCI	CCTCAGC	1 cut(s)	4368
BceAI	ACGGC	2 cut(s)	3755, 5490
BcgI	CGANNNNNNTGC	6 cut(s)	269, 303, 479, 513, 1748, 1782
BciT130I	CCWGG	7 cut(s)	273, 1440, 1470, 1907, 2761, 3170, 3933
BciVI	GTATCC	2 cut(s)	3689, 5497
BcnI	CCSGG	1 cut(s)	3360
BfmI	CTRYAG	7 cut(s)	1035, 1275, 1379, 1680, 3217, 3879, 4482
BfoI	RGCGCY	1 cut(s)	358
BfrI	CTTAAG	1 cut(s)	1481
BfuAI	ACCTGC	3 cut(s)	1579, 3802, 3886
BfuI	GTATCC	2 cut(s)	3689, 5497
BglII	AGATCT	5 cut(s)	259, 985, 4199, 5081, 5314
BlpI	GCTNAGC	3 cut(s)	2334, 2475, 4161
BmcAI	AGTACT	1 cut(s)	5342
Bme1390I	CCNGG	8 cut(s)	273, 1440, 1470, 1907, 2761, 3170, 3360, 3933
Bme18I	GGWCC	9 cut(s)	601, 1494, 2155, 3400, 3610, 3764, 4575, 4714, 5423
BmeRI	GACNNNNNGTC	4 cut(s)	1126, 2202, 3862, 5429
BmgBI	CACGTC	2 cut(s)	976, 1229
BmrFI	CCNGG	8 cut(s)	273, 1440, 1470, 1907, 2761, 3170, 3360, 3933
BmrI	ACTGGG	3 cut(s)	948, 2546, 5380
BmuI	ACTGGG	3 cut(s)	948, 2546, 5380
BpiI	GAAGAC	5 cut(s)	65, 437, 2584, 5013, 5313
BplI	GAGNNNNNCTC	8 cut(s)	110, 142, 276, 308, 3597, 3629, 5159, 5191
BpmI	CTGGAG	3 cut(s)	294, 573, 1913
Bpu10I	CCTNAGC	3 cut(s)	2270, 3981, 4368
Bpu1102I	GCTNAGC	3 cut(s)	2334, 2475, 4161
Bpu14I	TTCGAA	4 cut(s)	639, 1813, 3499, 5117
BpuEI	CTTGAG	5 cut(s)	240, 390, 2540, 3599, 5091
BpuMI	CCSGG	1 cut(s)	3360
BsaAI	YACGTR	2 cut(s)	3862, 5449
BsaBI	GATNNNNATC	1 cut(s)	4695
BsaI	GGTCTC	3 cut(s)	3284, 3586, 3849
BsaJI	CCNNGG	6 cut(s)	132, 712, 2181, 3168, 3169, 3932
BsaWI	WCCGGW	7 cut(s)	62, 85, 105, 1256, 1423, 1536, 4991
BsaXI	ACNNNNNCTCC	2 cut(s)	1229, 1259
Bse118I	RCCGGY	3 cut(s)	151, 1256, 4475
Bse21I	CCTNAGG	2 cut(s)	1249, 2526
Bse3DI	GCAATG	1 cut(s)	1518
Bse8I	GATNNNNATC	1 cut(s)	4695
BseAI	TCCGGA	2 cut(s)	1536, 4991
BseBI	CCWGG	7 cut(s)	273, 1440, 1470, 1907, 2761, 3170, 3933
BseDI	CCNNGG	6 cut(s)	132, 712, 2181, 3168, 3169, 3932
BseGI	GGATG	8 cut(s)	1776, 1969, 2182, 3211, 3682, 4867, 5194, 5539
BseJI	GATNNNNATC	1 cut(s)	4695
BseMI	GCAATG	1 cut(s)	1518
BseRI	GAGGAG	3 cut(s)	874, 1237, 4953
BseSI	GKGCMC	3 cut(s)	28, 3429, 4132
BseYI	CCCAGC	3 cut(s)	2186, 4154, 4214
BsgI	GTGCAG	3 cut(s)	1404, 2640, 3232
Bsh1236I	CGCG	1 cut(s)	4734
BshNI	GGYRCC	3 cut(s)	1635, 3953, 4258
BshTI	ACCGGT	1 cut(s)	1256
BsiHKAI	GWGCWC	6 cut(s)	28, 123, 289, 835, 2419, 3429
BslFI	GGGAC	9 cut(s)	986, 1480, 2758, 2810, 3187, 5216, 5222, 5228, 5368
BsmBI	CGTCTC	2 cut(s)	296, 1235
BsmFI	GGGAC	9 cut(s)	986, 1480, 2758, 2810, 3187, 5216, 5222, 5228, 5368
Bso31I	GGTCTC	3 cut(s)	3284, 3586, 3849
Bsp119I	TTCGAA	4 cut(s)	639, 1813, 3499, 5117
Bsp120I	GGGCCC	1 cut(s)	4128
Bsp1286I	GDGCHC	9 cut(s)	28, 123, 289, 835, 1247, 2419, 2805, 3429, 4132
Bsp13I	TCCGGA	2 cut(s)	1536, 4991
Bsp1720I	GCTNAGC	3 cut(s)	2334, 2475, 4161
Bsp19I	CCATGG	2 cut(s)	132, 2181
BspEI	TCCGGA	2 cut(s)	1536, 4991
BspFNI	CGCG	1 cut(s)	4734
BspMAI	CTGCAG	2 cut(s)	3883, 4486
BspMI	ACCTGC	3 cut(s)	1579, 3802, 3886
BspQI	GCTCTTC	1 cut(s)	2880
BspT104I	TTCGAA	4 cut(s)	639, 1813, 3499, 5117
BspT107I	GGYRCC	3 cut(s)	1635, 3953, 4258
BspTI	CTTAAG	1 cut(s)	1481
BspTNI	GGTCTC	3 cut(s)	3284, 3586, 3849
BsrBI	CCGCTC	3 cut(s)	170, 4712, 5230
BsrDI	GCAATG	1 cut(s)	1518
BsrFI	RCCGGY	3 cut(s)	151, 1256, 4475
BssAI	RCCGGY	3 cut(s)	151, 1256, 4475
BssECI	CCNNGG	6 cut(s)	132, 712, 2181, 3168, 3169, 3932
BssSI	CACGAG	1 cut(s)	2987
BssT1I	CCWWGG	3 cut(s)	132, 712, 2181
Bst2BI	CACGAG	1 cut(s)	2987
Bst2UI	CCWGG	7 cut(s)	273, 1440, 1470, 1907, 2761, 3170, 3933
BstAFI	CTTAAG	1 cut(s)	1481
BstBAI	YACGTR	2 cut(s)	3862, 5449
BstBI	TTCGAA	4 cut(s)	639, 1813, 3499, 5117
BstDSI	CCRYGG	2 cut(s)	132, 2181
BstENI	CCTNNNNNAGG	2 cut(s)	4184, 5261
BstF5I	GGATG	8 cut(s)	1776, 1969, 2182, 3211, 3682, 4867, 5194, 5539
BstFNI	CGCG	1 cut(s)	4734
BstH2I	RGCGCY	1 cut(s)	358
BstHHI	GCGC	3 cut(s)	357, 1936, 2371
BstNI	CCWGG	7 cut(s)	273, 1440, 1470, 1907, 2761, 3170, 3933
BstNSI	RCATGY	4 cut(s)	1545, 2999, 3124, 3358
BstSCI	CCNGG	8 cut(s)	271, 1438, 1468, 1905, 2759, 3168, 3358, 3931
BstSFI	CTRYAG	7 cut(s)	1035, 1275, 1379, 1680, 3217, 3879, 4482
BstSLI	GKGCMC	3 cut(s)	28, 3429, 4132
BstSNI	TACGTA	1 cut(s)	5449
BstUI	CGCG	1 cut(s)	4734
BstV2I	GAAGAC	5 cut(s)	65, 437, 2584, 5013, 5313
BstXI	CCANNNNNNTGG	1 cut(s)	313
Bsu36I	CCTNAGG	2 cut(s)	1249, 2526
BsuI	GTATCC	2 cut(s)	3689, 5497
BtgI	CCRYGG	2 cut(s)	132, 2181
BtgZI	GCGATG	2 cut(s)	525, 4632
BtrI	CACGTC	2 cut(s)	976, 1229
BtsCI	GGATG	8 cut(s)	1776, 1969, 2182, 3211, 3682, 4867, 5194, 5539
BtsI	GCAGTG	1 cut(s)	2452
BveI	ACCTGC	3 cut(s)	1579, 3802, 3886
CaiI	CAGNNNCTG	4 cut(s)	2648, 3131, 3472, 4118
CfoI	GCGC	3 cut(s)	357, 1936, 2371
Cfr10I	RCCGGY	3 cut(s)	151, 1256, 4475
CpoI	CGGWCCG	1 cut(s)	4714
CsiI	ACCWGGT	1 cut(s)	1438
CspAI	ACCGGT	1 cut(s)	1256
CspCI	CAANNNNNGTGG	2 cut(s)	2203, 2238
CspI	CGGWCCG	1 cut(s)	4714
DraI	TTTAAA	3 cut(s)	721, 1755, 3066
DrdI	GACNNNNNNGTC	2 cut(s)	659, 2501
DriI	GACNNNNNGTC	4 cut(s)	1126, 2202, 3862, 5429
DseDI	GACNNNNNNGTC	2 cut(s)	659, 2501
EaeI	YGGCCR	3 cut(s)	303, 3409, 3735
Eam1105I	GACNNNNNGTC	4 cut(s)	1126, 2202, 3862, 5429
EciI	GGCGGA	3 cut(s)	3816, 4088, 5314
Ecl136II	GAGCTC	2 cut(s)	121, 287
Eco105I	TACGTA	1 cut(s)	5449
Eco130I	CCWWGG	3 cut(s)	132, 712, 2181
Eco147I	AGGCCT	1 cut(s)	4027
Eco24I	GRGCYC	5 cut(s)	123, 289, 1247, 2805, 4132
Eco31I	GGTCTC	3 cut(s)	3284, 3586, 3849
Eco32I	GATATC	2 cut(s)	622, 4925
Eco47I	GGWCC	9 cut(s)	601, 1494, 2155, 3400, 3610, 3764, 4575, 4714, 5423
Eco47III	AGCGCT	1 cut(s)	356
Eco53kI	GAGCTC	2 cut(s)	121, 287
Eco72I	CACGTG	1 cut(s)	3862
Eco81I	CCTNAGG	2 cut(s)	1249, 2526
EcoICRI	GAGCTC	2 cut(s)	121, 287
EcoNI	CCTNNNNNAGG	2 cut(s)	4184, 5261
EcoO109I	RGGNCCY	6 cut(s)	1494, 2155, 3610, 4128, 4389, 5423
EcoRI	GAATTC	3 cut(s)	1674, 2662, 2943
EcoRII	CCWGG	7 cut(s)	271, 1438, 1468, 1905, 2759, 3168, 3931
EcoRV	GATATC	2 cut(s)	622, 4925
EcoT14I	CCWWGG	3 cut(s)	132, 712, 2181
EcoT22I	ATGCAT	1 cut(s)	1873
EcoT38I	GRGCYC	5 cut(s)	123, 289, 1247, 2805, 4132
ErhI	CCWWGG	3 cut(s)	132, 712, 2181
Esp3I	CGTCTC	2 cut(s)	296, 1235
FalI	AAGNNNNNCTT	4 cut(s)	4330, 4362, 4378, 4410
FaqI	GGGAC	9 cut(s)	986, 1480, 2758, 2810, 3187, 5216, 5222, 5228, 5368
FauI	CCCGC	3 cut(s)	18, 3390, 5223
FauNDI	CATATG	2 cut(s)	827, 1624
FblI	GTMKAC	3 cut(s)	1546, 1615, 2693
FokI	GGATG	8 cut(s)	1783, 1976, 2189, 3218, 3689, 4874, 5201, 5546
FriOI	GRGCYC	5 cut(s)	123, 289, 1247, 2805, 4132
GlaI	GCGC	3 cut(s)	356, 1935, 2370
GsaI	CCCAGC	3 cut(s)	2190, 4158, 4218
GsuI	CTGGAG	3 cut(s)	294, 573, 1913
HaeII	RGCGCY	1 cut(s)	358
HhaI	GCGC	3 cut(s)	357, 1936, 2371
Hin6I	GCGC	3 cut(s)	355, 1934, 2369
HinP1I	GCGC	3 cut(s)	355, 1934, 2369
HincII	GTYRAC	1 cut(s)	3196
HindII	GTYRAC	1 cut(s)	3196
HindIII	AAGCTT	4 cut(s)	226, 779, 2303, 4936
HspAI	GCGC	3 cut(s)	355, 1934, 2369
Kpn2I	TCCGGA	2 cut(s)	1536, 4991
KpnI	GGTACC	2 cut(s)	1639, 4262
LguI	GCTCTTC	1 cut(s)	2880
MabI	ACCWGGT	1 cut(s)	1438
MbiI	CCGCTC	3 cut(s)	170, 4712, 5230
MhlI	GDGCHC	9 cut(s)	28, 123, 289, 835, 1247, 2419, 2805, 3429, 4132
MlsI	TGGCCA	3 cut(s)	305, 3411, 3737
MluNI	TGGCCA	3 cut(s)	305, 3411, 3737
MmeI	TCCRAC	7 cut(s)	1567, 1692, 1748, 2697, 3873, 4575, 5514
Mox20I	TGGCCA	3 cut(s)	305, 3411, 3737
Mph1103I	ATGCAT	1 cut(s)	1873
MroI	TCCGGA	2 cut(s)	1536, 4991
MroXI	GAANNNNTTC	5 cut(s)	1326, 1674, 2943, 3084, 3661
MscI	TGGCCA	3 cut(s)	305, 3411, 3737
MslI	CAYNNNNRTG	5 cut(s)	48, 3204, 3206, 3951, 4914
Msp20I	TGGCCA	3 cut(s)	305, 3411, 3737
MspA1I	CMGCKG	6 cut(s)	1583, 1891, 2173, 3397, 4214, 4799
MspCI	CTTAAG	1 cut(s)	1481
MspR9I	CCNGG	8 cut(s)	273, 1440, 1470, 1907, 2761, 3170, 3360, 3933
MvaI	CCWGG	7 cut(s)	273, 1440, 1470, 1907, 2761, 3170, 3933
MvnI	CGCG	1 cut(s)	4734
NciI	CCSGG	1 cut(s)	3360
NcoI	CCATGG	2 cut(s)	132, 2181
NdeI	CATATG	2 cut(s)	827, 1624
NmuCI	GTSAC	5 cut(s)	976, 1128, 2012, 2408, 4728
NsiI	ATGCAT	1 cut(s)	1873
NspI	RCATGY	4 cut(s)	1545, 2999, 3124, 3358
NspV	TTCGAA	4 cut(s)	639, 1813, 3499, 5117
OliI	CACNNNNGTG	1 cut(s)	48
PaeI	GCATGC	1 cut(s)	3124
PasI	CCCWGGG	1 cut(s)	3169
PceI	AGGCCT	1 cut(s)	4027
PciI	ACATGT	2 cut(s)	1541, 2995
PciSI	GCTCTTC	1 cut(s)	2880
PcsI	WCGNNNNNNNCGW	2 cut(s)	3022, 5161
PdmI	GAANNNNTTC	5 cut(s)	1326, 1674, 2943, 3084, 3661
PflMI	CCANNNNNTGG	2 cut(s)	742, 1586
PfoI	TCCNGGA	1 cut(s)	1905
PinAI	ACCGGT	1 cut(s)	1256
PmaCI	CACGTG	1 cut(s)	3862
PmlI	CACGTG	1 cut(s)	3862
Ppu21I	YACGTR	2 cut(s)	3862, 5449
PpuMI	RGGWCCY	4 cut(s)	1494, 2155, 3610, 5423
PscI	ACATGT	2 cut(s)	1541, 2995
PshBI	ATTAAT	3 cut(s)	581, 2249, 3839
PsiI	TTATAA	3 cut(s)	572, 777, 4635
Psp124BI	GAGCTC	2 cut(s)	123, 289
Psp5II	RGGWCCY	4 cut(s)	1494, 2155, 3610, 5423
Psp6I	CCWGG	7 cut(s)	271, 1438, 1468, 1905, 2759, 3168, 3931
PspCI	CACGTG	1 cut(s)	3862
PspFI	CCCAGC	3 cut(s)	2186, 4154, 4214
PspGI	CCWGG	7 cut(s)	271, 1438, 1468, 1905, 2759, 3168, 3931
PspOMI	GGGCCC	1 cut(s)	4128
PspPPI	RGGWCCY	4 cut(s)	1494, 2155, 3610, 5423
PstI	CTGCAG	2 cut(s)	3883, 4486
PstNI	CAGNNNCTG	4 cut(s)	2648, 3131, 3472, 4118
PvuII	CAGCTG	5 cut(s)	1583, 1891, 2173, 4214, 4799
RseI	CAYNNNNRTG	5 cut(s)	48, 3204, 3206, 3951, 4914
Rsr2I	CGGWCCG	1 cut(s)	4714
RsrII	CGGWCCG	1 cut(s)	4714
SacI	GAGCTC	2 cut(s)	123, 289
SapI	GCTCTTC	1 cut(s)	2880
ScaI	AGTACT	1 cut(s)	5342
ScrFI	CCNGG	8 cut(s)	273, 1440, 1470, 1907, 2761, 3170, 3360, 3933
SduI	GDGCHC	9 cut(s)	28, 123, 289, 835, 1247, 2419, 2805, 3429, 4132
SexAI	ACCWGGT	1 cut(s)	1438
SfcI	CTRYAG	7 cut(s)	1035, 1275, 1379, 1680, 3217, 3879, 4482
SfuI	TTCGAA	4 cut(s)	639, 1813, 3499, 5117
SinI	GGWCC	9 cut(s)	601, 1494, 2155, 3400, 3610, 3764, 4575, 4714, 5423
SmiMI	CAYNNNNRTG	5 cut(s)	48, 3204, 3206, 3951, 4914
SmlI	CTYRAG	6 cut(s)	255, 405, 1481, 2519, 3614, 5106
SmoI	CTYRAG	6 cut(s)	255, 405, 1481, 2519, 3614, 5106
SnaBI	TACGTA	1 cut(s)	5449
SphI	GCATGC	1 cut(s)	3124
SseBI	AGGCCT	1 cut(s)	4027
SspI	AATATT	1 cut(s)	2916
SstI	GAGCTC	2 cut(s)	123, 289
StuI	AGGCCT	1 cut(s)	4027
StyD4I	CCNGG	8 cut(s)	271, 1438, 1468, 1905, 2759, 3168, 3358, 3931
StyI	CCWWGG	3 cut(s)	132, 712, 2181
TaqII	GACCGA	2 cut(s)	4581, 4650
TatI	WGTACW	5 cut(s)	1807, 3110, 3486, 4042, 5340
TauI	GCSGC	3 cut(s)	173, 1738, 4100
TseFI	GTSAC	5 cut(s)	976, 1128, 2012, 2408, 4728
Tsp45I	GTSAC	5 cut(s)	976, 1128, 2012, 2408, 4728
TspGWI	ACGGA	7 cut(s)	38, 2165, 2427, 3497, 4567, 4944, 5431
Van91I	CCANNNNNTGG	2 cut(s)	742, 1586
Vha464I	CTTAAG	1 cut(s)	1481
VneI	GTGCAC	2 cut(s)	24, 3425
VpaK11BI	GGWCC	9 cut(s)	601, 1494, 2155, 3400, 3610, 3764, 4575, 4714, 5423
VspI	ATTAAT	3 cut(s)	581, 2249, 3839
XagI	CCTNNNNNAGG	2 cut(s)	4184, 5261
XbaI	TCTAGA	1 cut(s)	4600
XceI	RCATGY	4 cut(s)	1545, 2999, 3124, 3358
XcmI	CCANNNNNNNNNTGG	2 cut(s)	313, 5393
XmiI	GTMKAC	3 cut(s)	1546, 1615, 2693
XmnI	GAANNNNTTC	5 cut(s)	1326, 1674, 2943, 3084, 3661
ZrmI	AGTACT	1 cut(s)	5342
Zsp2I	ATGCAT	1 cut(s)	1873

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.