AT1G27480

Lecithin:cholesterol acyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0015278
Physical Location & Seq
Forward (+)
9544410 .. 9546285
1876 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G27480.1

Sequence Viewer

Length: 1299 bp
ATGAAAAAAATATCTTCACATTATTCGGTAGTCATAGCGATACTCGTTGTGGTGACGATGACCTCGATGTGTCAAGCTGTGGGTAGCAACGTGTACCCTTTGATTCTGGTTCCAGGAAACGGAGGTAACCAGCTAGAGGTACGGCTGGACAGAGAATACAAGCCAAGTAGTGTCTGGTGTAGCAGCTGGTTATATCCGATTCATAAGAAGAGTGGTGGATGGTTTAGGCTATGGTTCGATGCAGCAGTGTTATTGTCTCCCTTCACCAGGTGCTTCAGCGATCGAATGATGTTGTACTATGACCCTGATTTGGATGATTACCAAAATGCTCCTGGTGTCCAAACCCGGGTTCCTCATTTCGGTTCGACCAAATCACTTCTATACCTCGACCCTCGTCTCCGAGATGCCACATCTTACATGGAACATTTGGTGAAAGCTCTAGAGAAAAAATGCGGGTATGTTAACGACCAAACCATCCTAGGAGCTCCATATGATTTCAGGTACGGCCTGGCTGCTTCGGGCCACCCGTCCCGTGTAGCCTCACAGTTCCTACAAGACCTCAAACAATTGGTGGAAAAAACTAGCAGCGAGAACGAAGGAAAGCCAGTGATACTCCTCTCCCATAGCCTAGGAGGACTTTTCGTCCTCCATTTCCTCAACCGTACCACCCCTTCATGGCGCCGCAAGTACATCAAACACTTTGTTGCACTCGCTGCGCCATGGGGTGGGACGATCTCTCAGATGAAGACATTTGCTTCTGGCAACACACTCGGTGTCCCTTTAGTTAACCCTTTGCTGGTCAGACGGCATCAGAGGACCTCCGAGAGTAACCAATGGCTACTTCCATCTACCAAAGTGTTTCACGACAGAACTAAACCGCTTGTCGTAACTCCCCAGGTTAACTACACAGCTTACGAGATGGATCGGTTTTTTGCAGACATTGGATTCTCACAAGGAGTTGTGCCTTACAAGACAAGAGTGTTGCCTTTAACAGAGGAGCTGATGACTCCGGGAGTGCCAGTCACTTGCATATATGGGAGAGGAGTTGATACACCGGAGGTTTTGATGTATGGAAAAGGAGGATTCGATAAGCAACCAGAGATTAAGTATGGAGATGGAGATGGGACGGTTAATTTGGCGAGCTTAGCAGCTTTGAAAGTCGATAGCTTGAACACCGTAGAGATTGATGGAGTTTCGCATACATCTATACTTAAAGACGAGATCGCACTTAAAGAGATTATGAAGCAGATTTCAATTATTAATTATGAATTAGCCAATGTTAATGCCGTCAATGAATGA

Protein Analysis

432

Amino Acids

48.31

Weight (kDa)

8.73

Isoelectric Point (pI)

33.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LCAT PF02450 75 - 314 2.4e-43 Lecithin:cholesterol acyltransferase
Hydrolase_4 PF12146 180 - 227 5.6e-08 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015278)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G27480
fragaria_vesca FvH4_2g32820 FvH4_2g32820
prunus_persica Prupe.1G420700_v2.0.a1
pyrus_communis pycom08g06610
rosa_chinensis RchiOBHm_Chr6g0309881
rosa_laevigata RLG00000010527
rosa_multiflora Rmu_sc0006780.1_g000001
rosa_roxburghii Rroxscaffold_7G00159080
rosa_rugosa Rorug06G0375600
rosa_samantha Rh6AG489300 Rh6AG489400 Rh6BG498800 Rh6CG503600 Rh6DG489700
rosa_wichuraiana Rw6G042590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 678
AccB7I CCANNNNNTGG 1 cut(s) 725
AciI CCGC 3 cut(s) 453, 682, 878
AclWI GGATC 1 cut(s) 930
AcuI CTGAAG 1 cut(s) 259
AcyI GRCGYC 1 cut(s) 679
AdeI CACNNNGTG 2 cut(s) 270, 773
AfaI GTAC 6 cut(s) 95, 141, 296, 503, 664, 689
AfiI CCNNNNNNNGG 9 cut(s) 119, 136, 267, 310, 346, 359, 675, 725, 796
AflIII ACRYGT 1 cut(s) 90
AgsI TTSAA 3 cut(s) 1156, 1171, 1254
AhdI GACNNNNNGTC 1 cut(s) 641
AjnI CCWGG 5 cut(s) 112, 266, 331, 507, 894
AjuI GAANNNNNNNTTGG 4 cut(s) 333, 365, 825, 857
Alw21I GWGCWC 1 cut(s) 487
Alw26I GTCTC 2 cut(s) 261, 401
AlwI GGATC 1 cut(s) 930
Ama87I CYCGRG 1 cut(s) 345
AoxI GGCC 2 cut(s) 505, 520
ApeKI GCWGC 6 cut(s) 183, 242, 512, 585, 713, 1148
ArsI GACNNNNNNTTYG 2 cut(s) 1117, 1149
AseI ATTAAT 1 cut(s) 1260
AspA2I CCTAGG 2 cut(s) 478, 628
AspLEI GCGC 2 cut(s) 681, 718
AspS9I GGNCC 2 cut(s) 520, 816
AsuC2I CCSGG 3 cut(s) 346, 347, 1011
AsuHPI GGTGA 3 cut(s) 64, 256, 442
AvaI CYCGRG 1 cut(s) 345
AvaII GGWCC 1 cut(s) 816
AvrII CCTAGG 2 cut(s) 478, 628
BanI GGYRCC 1 cut(s) 678
BanII GRGCYC 1 cut(s) 487
BarI GAAGNNNNNNTAC 2 cut(s) 655, 687
BbsI GAAGAC 1 cut(s) 752
Bbv12I GWGCWC 1 cut(s) 487
BbvI GCAGC 6 cut(s) 195, 254, 499, 597, 700, 1160
BccI CCATC 7 cut(s) 213, 482, 853, 913, 1109, 1115, 1181
BceAI ACGGC 4 cut(s) 158, 520, 821, 1271
BcgI CGANNNNNNTGC 2 cut(s) 751, 785
BciT130I CCWGG 5 cut(s) 114, 268, 333, 509, 896
BcnI CCSGG 3 cut(s) 346, 347, 1011
BcoDI GTCTC 2 cut(s) 261, 401
BfaI CTAG 5 cut(s) 134, 440, 479, 582, 629
BfoI RGCGCY 1 cut(s) 682
BisI GCNGC 7 cut(s) 184, 243, 513, 586, 682, 714, 1149
BlnI CCTAGG 2 cut(s) 478, 628
BlpI GCTNAGC 1 cut(s) 1144
BlsI GCNGC 7 cut(s) 185, 244, 514, 587, 683, 715, 1150
Bme1390I CCNGG 8 cut(s) 114, 268, 333, 346, 347, 509, 896, 1011
Bme18I GGWCC 1 cut(s) 816
BmeRI GACNNNNNGTC 1 cut(s) 641
BmeT110I CYCGRG 1 cut(s) 345
BmgT120I GGNCC 2 cut(s) 520, 816
BmiI GGNNCC 3 cut(s) 111, 351, 680
BmrFI CCNGG 8 cut(s) 114, 268, 333, 346, 347, 509, 896, 1011
BmsI GCATC 3 cut(s) 229, 394, 817
BoxI GACNNNNGTC 1 cut(s) 393
BpiI GAAGAC 1 cut(s) 752
Bpu1102I GCTNAGC 1 cut(s) 1144
BpuMI CCSGG 3 cut(s) 346, 347, 1011
BsaHI GRCGYC 1 cut(s) 679
BsaJI CCNNGG 5 cut(s) 345, 478, 628, 719, 894
BsaWI WCCGGW 1 cut(s) 1054
BsaXI ACNNNNNCTCC 2 cut(s) 1005, 1035
Bsc4I CCNNNNNNNGG 9 cut(s) 119, 136, 267, 310, 346, 359, 675, 725, 796
Bse1I ACTGG 2 cut(s) 605, 1019
BseBI CCWGG 5 cut(s) 114, 268, 333, 509, 896
BseDI CCNNGG 5 cut(s) 345, 478, 628, 719, 894
BseGI GGATG 3 cut(s) 224, 319, 474
BseLI CCNNNNNNNGG 9 cut(s) 119, 136, 267, 310, 346, 359, 675, 725, 796
BseMII CTCAG 1 cut(s) 752
BseNI ACTGG 2 cut(s) 605, 1019
BseRI GAGGAG 3 cut(s) 605, 1010, 1056
BseXI GCAGC 6 cut(s) 195, 254, 499, 597, 700, 1160
Bsh1285I CGRYCG 1 cut(s) 283
BshFI GGCC 2 cut(s) 507, 522
BshNI GGYRCC 1 cut(s) 678
BsiEI CGRYCG 1 cut(s) 283
BsiHKAI GWGCWC 1 cut(s) 487
BsiHKCI CYCGRG 1 cut(s) 345
BsiSI CCGG 3 cut(s) 346, 1010, 1055
BslFI GGGAC 4 cut(s) 514, 742, 761, 1138
BslI CCNNNNNNNGG 9 cut(s) 119, 136, 267, 310, 346, 359, 675, 725, 796
BsmAI GTCTC 2 cut(s) 261, 401
BsmBI CGTCTC 1 cut(s) 401
BsmFI GGGAC 4 cut(s) 514, 742, 761, 1138
BsnI GGCC 2 cut(s) 507, 522
BsoBI CYCGRG 1 cut(s) 345
Bsp1286I GDGCHC 1 cut(s) 487
Bsp143I GATC 4 cut(s) 280, 732, 922, 1221
Bsp1720I GCTNAGC 1 cut(s) 1144
Bsp19I CCATGG 1 cut(s) 719
BspACI CCGC 3 cut(s) 453, 682, 878
BspANI GGCC 2 cut(s) 507, 522
BspCNI CTCAG 1 cut(s) 751
BspLI GGNNCC 3 cut(s) 111, 351, 680
BspPI GGATC 1 cut(s) 930
BspT107I GGYRCC 1 cut(s) 678
BsrI ACTGG 2 cut(s) 605, 1019
BssECI CCNNGG 5 cut(s) 345, 478, 628, 719, 894
BssMI GATC 4 cut(s) 280, 732, 922, 1221
BssNI GRCGYC 1 cut(s) 679
BssT1I CCWWGG 3 cut(s) 478, 628, 719
Bst2UI CCWGG 5 cut(s) 114, 268, 333, 509, 896
Bst4CI ACNGT 4 cut(s) 546, 662, 1129, 1177
Bst6I CTCTTC 1 cut(s) 203
BstACI GRCGYC 1 cut(s) 679
BstAPI GCANNNNNTGC 1 cut(s) 713
BstC8I GCNNGC 1 cut(s) 1141
BstDEI CTNAG 2 cut(s) 738, 1144
BstDSI CCRYGG 1 cut(s) 719
BstEII GGTNACC 1 cut(s) 125
BstENI CCTNNNNNAGG 1 cut(s) 265
BstF5I GGATG 3 cut(s) 224, 319, 474
BstH2I RGCGCY 1 cut(s) 682
BstHHI GCGC 2 cut(s) 681, 718
BstKTI GATC 4 cut(s) 283, 735, 925, 1224
BstMAI GTCTC 2 cut(s) 261, 401
BstMBI GATC 4 cut(s) 280, 732, 922, 1221
BstMCI CGRYCG 1 cut(s) 283
BstMWI GCNNNNNNNGC 2 cut(s) 713, 1145
BstNI CCWGG 5 cut(s) 114, 268, 333, 509, 896
BstPAI GACNNNNGTC 1 cut(s) 393
BstPI GGTNACC 1 cut(s) 125
BstSCI CCNGG 8 cut(s) 112, 266, 331, 344, 345, 507, 894, 1009
BstV1I GCAGC 6 cut(s) 195, 254, 499, 597, 700, 1160
BstV2I GAAGAC 1 cut(s) 752
BsuRI GGCC 2 cut(s) 507, 522
BtgI CCRYGG 1 cut(s) 719
BtsCI GGATG 3 cut(s) 224, 319, 474
BtsI GCAGTG 1 cut(s) 252
BtsIMutI CAGTG 2 cut(s) 252, 612
Cac8I GCNNGC 1 cut(s) 1141
CfoI GCGC 2 cut(s) 681, 718
Cfr13I GGNCC 2 cut(s) 520, 816
Cfr9I CCCGGG 1 cut(s) 345
CsiI ACCWGGT 1 cut(s) 266
Csp6I GTAC 6 cut(s) 94, 140, 295, 502, 663, 688
CviAII CATG 3 cut(s) 418, 675, 720
CviQI GTAC 6 cut(s) 94, 140, 295, 502, 663, 688
DdeI CTNAG 2 cut(s) 738, 1144
DinI GGCGCC 1 cut(s) 680
DpnI GATC 4 cut(s) 282, 734, 924, 1223
DpnII GATC 4 cut(s) 280, 732, 922, 1221
DraIII CACNNNGTG 2 cut(s) 270, 773
DriI GACNNNNNGTC 1 cut(s) 641
Eam1104I CTCTTC 1 cut(s) 203
Eam1105I GACNNNNNGTC 1 cut(s) 641
EarI CTCTTC 1 cut(s) 203
Ecl136II GAGCTC 1 cut(s) 485
Eco130I CCWWGG 3 cut(s) 478, 628, 719
Eco24I GRGCYC 1 cut(s) 487
Eco47I GGWCC 1 cut(s) 816
Eco53kI GAGCTC 1 cut(s) 485
Eco57I CTGAAG 1 cut(s) 259
Eco88I CYCGRG 1 cut(s) 345
Eco91I GGTNACC 1 cut(s) 125
EcoICRI GAGCTC 1 cut(s) 485
EcoNI CCTNNNNNAGG 1 cut(s) 265
EcoO109I RGGNCCY 1 cut(s) 816
EcoO65I GGTNACC 1 cut(s) 125
EcoRII CCWGG 5 cut(s) 112, 266, 331, 507, 894
EcoT14I CCWWGG 3 cut(s) 478, 628, 719
EcoT38I GRGCYC 1 cut(s) 487
EgeI GGCGCC 1 cut(s) 680
EheI GGCGCC 1 cut(s) 680
ErhI CCWWGG 3 cut(s) 478, 628, 719
Esp3I CGTCTC 1 cut(s) 401
FaeI CATG 3 cut(s) 421, 678, 723
FaqI GGGAC 4 cut(s) 514, 742, 761, 1138
FatI CATG 3 cut(s) 417, 674, 719
FauI CCCGC 1 cut(s) 446
FauNDI CATATG 1 cut(s) 490
Fnu4HI GCNGC 7 cut(s) 184, 243, 513, 586, 682, 714, 1149
FokI GGATG 3 cut(s) 231, 326, 461
FriOI GRGCYC 1 cut(s) 487
Fsp4HI GCNGC 7 cut(s) 184, 243, 513, 586, 682, 714, 1149
FspBI CTAG 5 cut(s) 134, 440, 479, 582, 629
GlaI GCGC 2 cut(s) 680, 717
GluI GCNGC 7 cut(s) 184, 243, 513, 586, 682, 714, 1149
HaeII RGCGCY 1 cut(s) 682
HaeIII GGCC 2 cut(s) 507, 522
HapII CCGG 3 cut(s) 346, 1010, 1055
HhaI GCGC 2 cut(s) 681, 718
Hin1I GRCGYC 1 cut(s) 679
Hin1II CATG 3 cut(s) 421, 678, 723
Hin6I GCGC 2 cut(s) 679, 716
HinP1I GCGC 2 cut(s) 679, 716
HincII GTYRAC 3 cut(s) 463, 787, 901
HindII GTYRAC 3 cut(s) 463, 787, 901
HinfI GANTC 5 cut(s) 103, 199, 945, 1006, 1083
HpaI GTTAAC 3 cut(s) 463, 787, 901
HpaII CCGG 3 cut(s) 346, 1010, 1055
HphI GGTGA 3 cut(s) 64, 256, 442
Hpy166II GTNNAC 4 cut(s) 94, 463, 787, 901
Hpy188I TCNGA 6 cut(s) 198, 401, 741, 803, 813, 823
Hpy188III TCNNGA 2 cut(s) 440, 863
Hpy8I GTNNAC 4 cut(s) 94, 463, 787, 901
HpyAV CCTTC 3 cut(s) 271, 590, 681
HpyCH4III ACNGT 4 cut(s) 546, 662, 1129, 1177
HpyCH4IV ACGT 1 cut(s) 90
HpyCH4V TGCA 4 cut(s) 242, 707, 935, 1029
HpyF10VI GCNNNNNNNGC 2 cut(s) 713, 1145
HpyF3I CTNAG 2 cut(s) 738, 1144
HpySE526I ACGT 1 cut(s) 90
Hsp92I GRCGYC 1 cut(s) 679
Hsp92II CATG 3 cut(s) 421, 678, 723
HspAI GCGC 2 cut(s) 679, 716
KasI GGCGCC 1 cut(s) 678
KspAI GTTAAC 3 cut(s) 463, 787, 901
Kzo9I GATC 4 cut(s) 280, 732, 922, 1221
LmnI GCTCC 4 cut(s) 334, 482, 490, 997
Lsp1109I GCAGC 6 cut(s) 195, 254, 499, 597, 700, 1160
LweI GCATC 3 cut(s) 229, 394, 817
MabI ACCWGGT 1 cut(s) 266
MaeI CTAG 5 cut(s) 134, 440, 479, 582, 629
MaeII ACGT 1 cut(s) 90
MaeIII GTNAC 5 cut(s) 52, 125, 827, 886, 1021
MalI GATC 4 cut(s) 282, 734, 924, 1223
MboI GATC 4 cut(s) 280, 732, 922, 1221
MboII GAAGA 3 cut(s) 6, 220, 757
MfeI CAATTG 1 cut(s) 566
MhlI GDGCHC 1 cut(s) 487
MluCI AATT 5 cut(s) 566, 1132, 1254, 1261, 1268
Mly113I GGCGCC 1 cut(s) 679
MlyI GAGTC 1 cut(s) 1000
MspA1I CMGCKG 1 cut(s) 186
MspI CCGG 3 cut(s) 346, 1010, 1055
MspR9I CCNGG 8 cut(s) 114, 268, 333, 346, 347, 509, 896, 1011
MunI CAATTG 1 cut(s) 566
MvaI CCWGG 5 cut(s) 114, 268, 333, 509, 896
MwoI GCNNNNNNNGC 2 cut(s) 713, 1145
NarI GGCGCC 1 cut(s) 679
NciI CCSGG 3 cut(s) 346, 347, 1011
NcoI CCATGG 1 cut(s) 719
NdeI CATATG 1 cut(s) 490
NdeII GATC 4 cut(s) 280, 732, 922, 1221
NlaIII CATG 3 cut(s) 421, 678, 723
NlaIV GGNNCC 3 cut(s) 111, 351, 680
NmuCI GTSAC 2 cut(s) 52, 1021
PcsI WCGNNNNNNNCGW 2 cut(s) 62, 524
PfeI GAWTC 4 cut(s) 103, 199, 945, 1083
PflMI CCANNNNNTGG 1 cut(s) 725
PfoI TCCNGGA 2 cut(s) 112, 1009
PkrI GCNGC 7 cut(s) 185, 244, 514, 587, 683, 715, 1150
Ple19I CGATCG 1 cut(s) 283
PleI GAGTC 1 cut(s) 1000
PluTI GGCGCC 1 cut(s) 682
PpsI GAGTC 1 cut(s) 1000
PpuMI RGGWCCY 1 cut(s) 816
PshAI GACNNNNGTC 1 cut(s) 393
PshBI ATTAAT 1 cut(s) 1260
Psp124BI GAGCTC 1 cut(s) 487
Psp5II RGGWCCY 1 cut(s) 816
Psp6I CCWGG 5 cut(s) 112, 266, 331, 507, 894
PspEI GGTNACC 1 cut(s) 125
PspGI CCWGG 5 cut(s) 112, 266, 331, 507, 894
PspN4I GGNNCC 3 cut(s) 111, 351, 680
PspPI GGNCC 2 cut(s) 520, 816
PspPPI RGGWCCY 1 cut(s) 816
PvuI CGATCG 1 cut(s) 283
PvuII CAGCTG 1 cut(s) 186
RsaI GTAC 6 cut(s) 95, 141, 296, 503, 664, 689
RsaNI GTAC 6 cut(s) 94, 140, 295, 502, 663, 688
SacI GAGCTC 1 cut(s) 487
SatI GCNGC 7 cut(s) 184, 243, 513, 586, 682, 714, 1149
Sau3AI GATC 4 cut(s) 280, 732, 922, 1221
Sau96I GGNCC 2 cut(s) 520, 816
SchI GAGTC 1 cut(s) 1000
ScrFI CCNGG 8 cut(s) 114, 268, 333, 346, 347, 509, 896, 1011
SduI GDGCHC 1 cut(s) 487
SexAI ACCWGGT 1 cut(s) 266
SfaNI GCATC 3 cut(s) 229, 394, 817
SfoI GGCGCC 1 cut(s) 680
SinI GGWCC 1 cut(s) 816
SmaI CCCGGG 1 cut(s) 347
Sse9I AATT 5 cut(s) 566, 1132, 1254, 1261, 1268
SsiI CCGC 3 cut(s) 453, 682, 878
SspDI GGCGCC 1 cut(s) 678
SspMI CTAG 5 cut(s) 134, 440, 479, 582, 629
SstI GAGCTC 1 cut(s) 487
StyD4I CCNGG 8 cut(s) 112, 266, 331, 344, 345, 507, 894, 1009
StyI CCWWGG 3 cut(s) 478, 628, 719
TaaI ACNGT 4 cut(s) 546, 662, 1129, 1177
TaiI ACGT 1 cut(s) 93
TaqI TCGA 7 cut(s) 65, 237, 283, 365, 387, 1086, 1161
TasI AATT 5 cut(s) 566, 1132, 1254, 1261, 1268
TatI WGTACW 2 cut(s) 294, 687
TauI GCSGC 1 cut(s) 684
TfiI GAWTC 4 cut(s) 103, 199, 945, 1083
TscAI CASTG 2 cut(s) 252, 612
TseFI GTSAC 2 cut(s) 52, 1021
TseI GCWGC 6 cut(s) 183, 242, 512, 585, 713, 1148
Tsp45I GTSAC 2 cut(s) 52, 1021
TspDTI ATGAA 6 cut(s) 17, 191, 663, 758, 1256, 1281
TspGWI ACGGA 1 cut(s) 135
TspMI CCCGGG 1 cut(s) 345
TspRI CASTG 2 cut(s) 252, 612
Van91I CCANNNNNTGG 1 cut(s) 725
VpaK11BI GGWCC 1 cut(s) 816
VspI ATTAAT 1 cut(s) 1260
XagI CCTNNNNNAGG 1 cut(s) 265
XbaI TCTAGA 1 cut(s) 439
XcmI CCANNNNNNNNNTGG 3 cut(s) 171, 329, 415
XmaI CCCGGG 1 cut(s) 345
XmaJI CCTAGG 2 cut(s) 478, 628
XspI CTAG 5 cut(s) 134, 440, 479, 582, 629
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.