Rorug06G0375600

Lecithin-cholesterol acyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0015278
Physical Location & Seq
Forward (+)
52239288 .. 52241915
2628 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0375600.1

Sequence Viewer

Length: 1461 bp
ATGGGTACTGTTCTTCCCCAACACCAGGCTTTTGATGTTTCTGTGGATGTGCAACCACAGAGTGGCTCGAAGTGTTTCGATGACGATGGCCGTCTCAAACGAACTGGAACTGTTTGGACTGCAAGTGCTCATATTATCACCGCTGTGATTGGTTCTGGTGTGCTGGCCTTGGCTTGGGCCATAGCTCAACTTGGCTGGGTTGCTGGTCCTGCCGTGATGCTCTTGTTCTCTTTTGTCACTTACTACACATCTAGTCTTCTCTCTGCTTGTTATCGTACCGGTGATCCTGTCACTGGCAAGAGAAATTACACTTACACAGATGCTGTCAGATCCAACCTTGGTGGGTTCAAGGTGAAGATTTGTGGGTTTGTTCAGTACTTGAATCTTTTTGGAGTTGCTATTGGCTATACTATAGCATCATCTATAAGCATGGTGGCTATAAAGAGGTCTAACTGCTTCCACAGCAGTGATGGAAAAGATGAATGCCATGTGAACAGCAATCCTTATATGATTGCTTTTGGCATTGCAGAAATCATATTCTCCCAAATTGAAAACTTTGATCAGCTATGGTGGCTCTCCATTGTTGCTGCTGTCATGTCCTTCACTTACTCCTCTATTGGACTTGGCCTTGGAATTGGGAAAGTTGCAGAAACTGGAACAATTAGGGGAAGCATGACTGGAATAGACATTGGAACAGTGATTGGGACTCAAAAGATAACTGAGACTCTAAAGATATGGAGGAGCTTCCAAGCACTTGGTGACATAGCCTTTGCCTACTCCTACTCTCTCATCCTGATTGAAATTCAGGACACTGTCAAATCCCCACCGTCCGAAGCCAAGACGATGAGGAAGGCGACTCTAGTCAGTGTGGCAACCACAACCCTTTTCTACATGCTGTGTGGCTGCATGGGCTATGCTGCTTTTGGGGATGCATCCCCCACAAACCTCCTCACCGGTTTCGGTTTCTACAACCCTTACTGGCTCATTGACATTGCCAATGCTGCCATAGTCATCCATCTTGTTGGTGCCTACCAAGTCTTTGTCCAACCCCTCTTTGCTTTTGTTGAGAAATCAGCAGCAGAGAAGTACCCAGATAGCCAATTCATCACCAAAAACATCAAAATCCAAATCCCTTGTGTCGGTCTTTACAACCTCAATATGTTCAGATTGGTGTGGAGGACACTGTTTGTGATCACAACCACTGTGATTTCCATGATCCTTCCATTCTTTAACGACGTTGTTGGACTTCTTGGCGCTTTGGGATTTTGGCCACTCACAGTATACTTCCCAGTGGAGATGTACATTGTCCAGAAGAGGATTCCAAAATGGAGCACCAAATGGCTTTGCCTCCAAACCCTAAGTGGTGCTTGCCTCATAATCTCCATAGCTGCTGCTGCTGGCTCAATTGCCGGGGTGCTTTCCGACCTCAAGACCTACAAGCCATTCCAATCCAGCGATTGA

Protein Analysis

486

Amino Acids

53.05

Weight (kDa)

8.54

Isoelectric Point (pI)

35.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 34 - 473 2.7e-121 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015278)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G27480
fragaria_vesca FvH4_2g32820 FvH4_2g32820
prunus_persica Prupe.1G420700_v2.0.a1
pyrus_communis pycom08g06610
rosa_chinensis RchiOBHm_Chr6g0309881
rosa_laevigata RLG00000010527
rosa_multiflora Rmu_sc0006780.1_g000001
rosa_roxburghii Rroxscaffold_7G00159080
rosa_rugosa Rorug06G0375600
rosa_samantha Rh6AG489300 Rh6AG489400 Rh6BG498800 Rh6CG503600 Rh6DG489700
rosa_wichuraiana Rw6G042590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1025
AccB7I CCANNNNNTGG 1 cut(s) 62
AccI GTMKAC 1 cut(s) 1281
AciI CCGC 1 cut(s) 141
AclWI GGATC 3 cut(s) 278, 324, 1210
AcoI YGGCCR 2 cut(s) 88, 1268
AcsI RAATTY 1 cut(s) 801
AdeI CACNNNGTG 2 cut(s) 62, 758
AfaI GTAC 5 cut(s) 7, 277, 377, 1088, 1301
AfiI CCNNNNNNNGG 5 cut(s) 25, 62, 174, 293, 1139
AgeI ACCGGT 2 cut(s) 278, 953
AgsI TTSAA 4 cut(s) 349, 382, 551, 800
AjnI CCWGG 1 cut(s) 24
AleI CACNNNNGTG 2 cut(s) 143, 465
AluBI AGCT 4 cut(s) 185, 565, 744, 1388
AluI AGCT 4 cut(s) 185, 565, 744, 1388
Alw21I GWGCWC 2 cut(s) 130, 1334
Alw26I GTCTC 2 cut(s) 98, 716
AlwI GGATC 3 cut(s) 278, 324, 1210
AlwNI CAGNNNCTG 2 cut(s) 323, 653
AoxI GGCC 5 cut(s) 88, 165, 177, 625, 1268
ApeKI GCWGC 8 cut(s) 587, 903, 917, 1001, 1076, 1388, 1391, 1394
ApoI RAATTY 1 cut(s) 801
ArsI GACNNNNNNTTYG 2 cut(s) 752, 784
AsiGI ACCGGT 2 cut(s) 278, 953
Asp700I GAANNNNTTC 1 cut(s) 74
AspLEI GCGC 1 cut(s) 1256
AspS9I GGNCC 2 cut(s) 177, 206
AsuC2I CCSGG 1 cut(s) 1411
AsuHPI GGTGA 6 cut(s) 130, 293, 364, 770, 943, 1099
AvaII GGWCC 1 cut(s) 206
BalI TGGCCA 1 cut(s) 1270
BanI GGYRCC 1 cut(s) 1025
BbsI GAAGAC 1 cut(s) 248
Bbv12I GWGCWC 2 cut(s) 130, 1334
BbvI GCAGC 8 cut(s) 574, 890, 904, 988, 1088, 1375, 1378, 1381
BccI CCATC 3 cut(s) 80, 464, 1023
BceAI ACGGC 2 cut(s) 75, 197
BciT130I CCWGG 1 cut(s) 26
BclI TGATCA 2 cut(s) 559, 1191
BcnI CCSGG 1 cut(s) 1411
BcoDI GTCTC 2 cut(s) 98, 716
BfaI CTAG 2 cut(s) 252, 860
BfmI CTRYAG 1 cut(s) 411
BfoI RGCGCY 1 cut(s) 1257
BisI GCNGC 8 cut(s) 588, 904, 918, 1002, 1077, 1389, 1392, 1395
BlsI GCNGC 8 cut(s) 589, 905, 919, 1003, 1078, 1390, 1393, 1396
BmcAI AGTACT 1 cut(s) 377
Bme1390I CCNGG 2 cut(s) 26, 1411
Bme18I GGWCC 1 cut(s) 206
BmgT120I GGNCC 2 cut(s) 177, 206
BmiI GGNNCC 1 cut(s) 1027
BmrFI CCNGG 2 cut(s) 26, 1411
BmrI ACTGGG 1 cut(s) 1283
BmsI GCATC 5 cut(s) 207, 310, 425, 919, 941
BmuI ACTGGG 1 cut(s) 1283
BoxI GACNNNNGTC 1 cut(s) 860
BpiI GAAGAC 1 cut(s) 248
BpuEI CTTGAG 1 cut(s) 1412
BpuMI CCSGG 1 cut(s) 1411
BsaJI CCNNGG 4 cut(s) 168, 337, 628, 1410
BsaWI WCCGGW 2 cut(s) 278, 953
Bsc4I CCNNNNNNNGG 5 cut(s) 25, 62, 174, 293, 1139
Bse118I RCCGGY 2 cut(s) 278, 953
Bse1I ACTGG 6 cut(s) 109, 298, 658, 682, 983, 1289
Bse3DI GCAATG 2 cut(s) 522, 990
BseBI CCWGG 1 cut(s) 26
BseDI CCNNGG 4 cut(s) 168, 337, 628, 1410
BseGI GGATG 5 cut(s) 52, 789, 932, 934, 1011
BseLI CCNNNNNNNGG 5 cut(s) 25, 62, 174, 293, 1139
BseMI GCAATG 2 cut(s) 522, 990
BseMII CTCAG 1 cut(s) 711
BseNI ACTGG 6 cut(s) 109, 298, 658, 682, 983, 1289
BseRI GAGGAG 3 cut(s) 601, 754, 938
BseXI GCAGC 8 cut(s) 574, 890, 904, 988, 1088, 1375, 1378, 1381
BseYI CCCAGC 1 cut(s) 195
BshFI GGCC 5 cut(s) 90, 167, 179, 627, 1270
BshNI GGYRCC 1 cut(s) 1025
BshTI ACCGGT 2 cut(s) 278, 953
BsiHKAI GWGCWC 2 cut(s) 130, 1334
BsiSI CCGG 3 cut(s) 279, 954, 1410
BslFI GGGAC 1 cut(s) 718
BslI CCNNNNNNNGG 5 cut(s) 25, 62, 174, 293, 1139
BsmAI GTCTC 2 cut(s) 98, 716
BsmBI CGTCTC 1 cut(s) 98
BsmFI GGGAC 1 cut(s) 718
BsmI GAATGC 1 cut(s) 488
BsnI GGCC 5 cut(s) 90, 167, 179, 627, 1270
Bsp1286I GDGCHC 2 cut(s) 130, 1334
Bsp1407I TGTACA 1 cut(s) 1299
Bsp143I GATC 5 cut(s) 283, 329, 559, 1191, 1215
BspACI CCGC 1 cut(s) 141
BspANI GGCC 5 cut(s) 90, 167, 179, 627, 1270
BspCNI CTCAG 1 cut(s) 712
BspLI GGNNCC 1 cut(s) 1027
BspPI GGATC 3 cut(s) 278, 324, 1210
BspT107I GGYRCC 1 cut(s) 1025
BsrDI GCAATG 2 cut(s) 522, 990
BsrFI RCCGGY 2 cut(s) 278, 953
BsrGI TGTACA 1 cut(s) 1299
BsrI ACTGG 6 cut(s) 109, 298, 658, 682, 983, 1289
BssAI RCCGGY 2 cut(s) 278, 953
BssECI CCNNGG 4 cut(s) 168, 337, 628, 1410
BssMI GATC 5 cut(s) 283, 329, 559, 1191, 1215
BssNAI GTATAC 1 cut(s) 1282
BssT1I CCWWGG 3 cut(s) 168, 337, 628
Bst1107I GTATAC 1 cut(s) 1282
Bst2UI CCWGG 1 cut(s) 26
Bst4CI ACNGT 8 cut(s) 10, 112, 697, 814, 828, 1185, 1204, 1279
Bst6I CTCTTC 1 cut(s) 1307
BstAUI TGTACA 1 cut(s) 1299
BstC8I GCNNGC 3 cut(s) 165, 1369, 1399
BstDEI CTNAG 2 cut(s) 720, 1358
BstF5I GGATG 5 cut(s) 52, 789, 932, 934, 1011
BstH2I RGCGCY 1 cut(s) 1257
BstHHI GCGC 1 cut(s) 1256
BstKTI GATC 5 cut(s) 286, 332, 562, 1194, 1218
BstMAI GTCTC 2 cut(s) 98, 716
BstMBI GATC 5 cut(s) 283, 329, 559, 1191, 1215
BstMWI GCNNNNNNNGC 6 cut(s) 209, 462, 571, 909, 1001, 1394
BstNI CCWGG 1 cut(s) 26
BstNSI RCATGY 1 cut(s) 895
BstPAI GACNNNNGTC 1 cut(s) 860
BstSCI CCNGG 2 cut(s) 24, 1409
BstSFI CTRYAG 1 cut(s) 411
BstV1I GCAGC 8 cut(s) 574, 890, 904, 988, 1088, 1375, 1378, 1381
BstV2I GAAGAC 1 cut(s) 248
BstX2I RGATCY 1 cut(s) 329
BstXI CCANNNNNNTGG 2 cut(s) 755, 1022
BstYI RGATCY 1 cut(s) 329
BstZ17I GTATAC 1 cut(s) 1282
BsuRI GGCC 5 cut(s) 90, 167, 179, 627, 1270
BtsCI GGATG 5 cut(s) 52, 789, 932, 934, 1011
BtsI GCAGTG 1 cut(s) 472
BtsIMutI CAGTG 8 cut(s) 291, 472, 702, 810, 871, 1181, 1200, 1296
Cac8I GCNNGC 3 cut(s) 165, 1369, 1399
CaiI CAGNNNCTG 2 cut(s) 323, 653
CfoI GCGC 1 cut(s) 1256
Cfr10I RCCGGY 2 cut(s) 278, 953
Cfr13I GGNCC 2 cut(s) 177, 206
Csp6I GTAC 5 cut(s) 6, 276, 376, 1087, 1300
CspAI ACCGGT 2 cut(s) 278, 953
CspCI CAANNNNNGTGG 2 cut(s) 322, 357
CviAII CATG 7 cut(s) 430, 488, 595, 673, 892, 907, 1213
CviQI GTAC 5 cut(s) 6, 276, 376, 1087, 1300
DdeI CTNAG 2 cut(s) 720, 1358
DpnI GATC 5 cut(s) 285, 331, 561, 1193, 1217
DpnII GATC 5 cut(s) 283, 329, 559, 1191, 1215
DraIII CACNNNGTG 2 cut(s) 62, 758
EaeI YGGCCR 2 cut(s) 88, 1268
Eam1104I CTCTTC 1 cut(s) 1307
EarI CTCTTC 1 cut(s) 1307
Eco130I CCWWGG 3 cut(s) 168, 337, 628
Eco47I GGWCC 1 cut(s) 206
EcoRII CCWGG 1 cut(s) 24
EcoT14I CCWWGG 3 cut(s) 168, 337, 628
EcoT22I ATGCAT 1 cut(s) 934
ErhI CCWWGG 3 cut(s) 168, 337, 628
Esp3I CGTCTC 1 cut(s) 98
FaeI CATG 7 cut(s) 433, 491, 598, 676, 895, 910, 1216
FalI AAGNNNNNCTT 2 cut(s) 1351, 1383
FaqI GGGAC 1 cut(s) 718
FatI CATG 7 cut(s) 429, 487, 594, 672, 891, 906, 1212
FbaI TGATCA 2 cut(s) 559, 1191
FblI GTMKAC 1 cut(s) 1281
Fnu4HI GCNGC 8 cut(s) 588, 904, 918, 1002, 1077, 1389, 1392, 1395
FokI GGATG 5 cut(s) 59, 776, 919, 941, 998
Fsp4HI GCNGC 8 cut(s) 588, 904, 918, 1002, 1077, 1389, 1392, 1395
FspBI CTAG 2 cut(s) 252, 860
GlaI GCGC 1 cut(s) 1255
GluI GCNGC 8 cut(s) 588, 904, 918, 1002, 1077, 1389, 1392, 1395
GsaI CCCAGC 1 cut(s) 199
HaeII RGCGCY 1 cut(s) 1257
HaeIII GGCC 5 cut(s) 90, 167, 179, 627, 1270
HapII CCGG 3 cut(s) 279, 954, 1410
HhaI GCGC 1 cut(s) 1256
Hin1II CATG 7 cut(s) 433, 491, 598, 676, 895, 910, 1216
Hin6I GCGC 1 cut(s) 1254
HinP1I GCGC 1 cut(s) 1254
HinfI GANTC 5 cut(s) 382, 706, 724, 856, 1318
HpaII CCGG 3 cut(s) 279, 954, 1410
HphI GGTGA 6 cut(s) 130, 293, 364, 770, 943, 1099
Hpy166II GTNNAC 2 cut(s) 493, 1282
Hpy188I TCNGA 4 cut(s) 329, 832, 1166, 1423
Hpy188III TCNNGA 4 cut(s) 793, 806, 1309, 1429
Hpy8I GTNNAC 2 cut(s) 493, 1282
Hpy99I CGWCG 1 cut(s) 1238
HpyAV CCTTC 3 cut(s) 610, 844, 1229
HpyCH4III ACNGT 8 cut(s) 10, 112, 697, 814, 828, 1185, 1204, 1279
HpyCH4IV ACGT 1 cut(s) 1236
HpyCH4V TGCA 6 cut(s) 52, 122, 527, 647, 906, 932
HpyF10VI GCNNNNNNNGC 6 cut(s) 209, 462, 571, 909, 1001, 1394
HpyF3I CTNAG 2 cut(s) 720, 1358
HpySE526I ACGT 1 cut(s) 1236
Hsp92II CATG 7 cut(s) 433, 491, 598, 676, 895, 910, 1216
HspAI GCGC 1 cut(s) 1254
Ksp22I TGATCA 2 cut(s) 559, 1191
Kzo9I GATC 5 cut(s) 283, 329, 559, 1191, 1215
LmnI GCTCC 2 cut(s) 741, 1329
Lsp1109I GCAGC 8 cut(s) 574, 890, 904, 988, 1088, 1375, 1378, 1381
LweI GCATC 5 cut(s) 207, 310, 425, 919, 941
MaeI CTAG 2 cut(s) 252, 860
MaeII ACGT 1 cut(s) 1236
MaeIII GTNAC 3 cut(s) 235, 289, 758
MalI GATC 5 cut(s) 285, 331, 561, 1193, 1217
MboI GATC 5 cut(s) 283, 329, 559, 1191, 1215
MboII GAAGA 4 cut(s) 5, 248, 367, 1324
MfeI CAATTG 1 cut(s) 1404
MflI RGATCY 1 cut(s) 329
MhlI GDGCHC 2 cut(s) 130, 1334
MlsI TGGCCA 1 cut(s) 1270
MluCI AATT 7 cut(s) 304, 546, 633, 660, 801, 1100, 1404
MluNI TGGCCA 1 cut(s) 1270
MlyI GAGTC 3 cut(s) 700, 718, 850
MmeI TCCRAC 4 cut(s) 357, 1069, 1222, 1446
Mox20I TGGCCA 1 cut(s) 1270
Mph1103I ATGCAT 1 cut(s) 934
MroXI GAANNNNTTC 1 cut(s) 74
MscI TGGCCA 1 cut(s) 1270
MseI TTAA 1 cut(s) 1230
MslI CAYNNNNRTG 2 cut(s) 143, 465
Msp20I TGGCCA 1 cut(s) 1270
MspA1I CMGCKG 1 cut(s) 143
MspI CCGG 3 cut(s) 279, 954, 1410
MspR9I CCNGG 2 cut(s) 26, 1411
MunI CAATTG 1 cut(s) 1404
Mva1269I GAATGC 1 cut(s) 488
MvaI CCWGG 1 cut(s) 26
MwoI GCNNNNNNNGC 6 cut(s) 209, 462, 571, 909, 1001, 1394
NciI CCSGG 1 cut(s) 1411
NdeII GATC 5 cut(s) 283, 329, 559, 1191, 1215
NlaIII CATG 7 cut(s) 433, 491, 598, 676, 895, 910, 1216
NlaIV GGNNCC 1 cut(s) 1027
NmuCI GTSAC 3 cut(s) 235, 289, 758
NsiI ATGCAT 1 cut(s) 934
NspI RCATGY 1 cut(s) 895
OliI CACNNNNGTG 2 cut(s) 143, 465
PctI GAATGC 1 cut(s) 488
PdmI GAANNNNTTC 1 cut(s) 74
PfeI GAWTC 2 cut(s) 382, 1318
PflFI GACNNNGTC 1 cut(s) 812
PflMI CCANNNNNTGG 1 cut(s) 62
PinAI ACCGGT 2 cut(s) 278, 953
PkrI GCNGC 8 cut(s) 589, 905, 919, 1003, 1078, 1390, 1393, 1396
PleI GAGTC 3 cut(s) 700, 718, 850
PpsI GAGTC 3 cut(s) 700, 718, 850
PshAI GACNNNNGTC 1 cut(s) 860
Psp6I CCWGG 1 cut(s) 24
PspFI CCCAGC 1 cut(s) 195
PspGI CCWGG 1 cut(s) 24
PspN4I GGNNCC 1 cut(s) 1027
PspPI GGNCC 2 cut(s) 177, 206
PstNI CAGNNNCTG 2 cut(s) 323, 653
PsuI RGATCY 1 cut(s) 329
PsyI GACNNNGTC 1 cut(s) 812
RsaI GTAC 5 cut(s) 7, 277, 377, 1088, 1301
RsaNI GTAC 5 cut(s) 6, 276, 376, 1087, 1300
RseI CAYNNNNRTG 2 cut(s) 143, 465
SaqAI TTAA 1 cut(s) 1230
SatI GCNGC 8 cut(s) 588, 904, 918, 1002, 1077, 1389, 1392, 1395
Sau3AI GATC 5 cut(s) 283, 329, 559, 1191, 1215
Sau96I GGNCC 2 cut(s) 177, 206
ScaI AGTACT 1 cut(s) 377
SchI GAGTC 3 cut(s) 700, 718, 850
ScrFI CCNGG 2 cut(s) 26, 1411
SduI GDGCHC 2 cut(s) 130, 1334
SfaNI GCATC 5 cut(s) 207, 310, 425, 919, 941
SfcI CTRYAG 1 cut(s) 411
SinI GGWCC 1 cut(s) 206
SmiMI CAYNNNNRTG 2 cut(s) 143, 465
SmlI CTYRAG 1 cut(s) 1427
SmoI CTYRAG 1 cut(s) 1427
Sse9I AATT 7 cut(s) 304, 546, 633, 660, 801, 1100, 1404
SsiI CCGC 1 cut(s) 141
SspMI CTAG 2 cut(s) 252, 860
StyD4I CCNGG 2 cut(s) 24, 1409
StyI CCWWGG 3 cut(s) 168, 337, 628
TaaI ACNGT 8 cut(s) 10, 112, 697, 814, 828, 1185, 1204, 1279
TaiI ACGT 1 cut(s) 1239
TaqI TCGA 2 cut(s) 68, 78
TaqII GACCGA 1 cut(s) 1130
TasI AATT 7 cut(s) 304, 546, 633, 660, 801, 1100, 1404
TatI WGTACW 2 cut(s) 375, 1299
TfiI GAWTC 2 cut(s) 382, 1318
Tru1I TTAA 1 cut(s) 1230
Tru9I TTAA 1 cut(s) 1230
TscAI CASTG 8 cut(s) 298, 472, 702, 817, 871, 1188, 1207, 1296
TseFI GTSAC 3 cut(s) 235, 289, 758
TseI GCWGC 8 cut(s) 587, 903, 917, 1001, 1076, 1388, 1391, 1394
Tsp45I GTSAC 3 cut(s) 235, 289, 758
TspDTI ATGAA 2 cut(s) 495, 1093
TspRI CASTG 8 cut(s) 298, 472, 702, 817, 871, 1188, 1207, 1296
Tth111I GACNNNGTC 1 cut(s) 812
Van91I CCANNNNNTGG 1 cut(s) 62
VpaK11BI GGWCC 1 cut(s) 206
XapI RAATTY 1 cut(s) 801
XceI RCATGY 1 cut(s) 895
XcmI CCANNNNNNNNNTGG 2 cut(s) 467, 1358
XmiI GTMKAC 1 cut(s) 1281
XmnI GAANNNNTTC 1 cut(s) 74
XspI CTAG 2 cut(s) 252, 860
ZrmI AGTACT 1 cut(s) 377
Zsp2I ATGCAT 1 cut(s) 934
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.