Rh6AG489400

Lecithin-cholesterol acyltransferase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0015278
Physical Location & Seq
Forward (+)
66633664 .. 66638706
5043 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG489400.1

Sequence Viewer

Length: 1026 bp
ATGGCACCCCTAGTGGAATCATTGGAGAATATTGGCTATGTCGATGGCAAAACCATGTTCGGAGCTCCATACGATTTTCGATATGGTTTAGCACCTAAAGACCACCCATCCCGTGTTGGTTCTAAGTTCCTGAAAGACCTAAAAGACTTGGTCGAAAAGGCCGGCAATTCTAATGGAGGAAAGCCGGTGATACTTGTCTCGCACAGCTTAGGAGGCCTCTTTGCTCTCCAACTTCTGAATCGAAACAAACCCTCATGGCGCCAAAAGTTCATCAAACACGTTGTTGCACTCTCAGCACCGTGGGGTGGGACTGTGGACGAAATGCTCACCTTTGCTTCAGGGAACACACTAGGAGTGCCACTTGTGAATCCATTGCTCGTAAGAGAAGAGCAGAGGAGCTCCGAAAGCAACTTATGGCTAATGCCTAATCCTAGATTATTCAGGAGAAAAAAACCAATAGTGATCACCCCAGATGCTACATATTCAGCTCATAACATTCCCCAATTCCTCAATGACATTGGATTTCCCGAAGGAATTCATCCCTTCAAAACGCGCATTTTGGTCTTGATGGATCAGCTTGAAGCTCCTGGGGTGCCTCTTACTTGTGTTATTGGGACCGGTGTGAGGACAGCAGAGACTTTGTTCTATGACAAAAGAGGCTTTGATGAGCAACCGGAGATTGTTTACGGGGATGGAGATGGAACTGTGAACATGTTGAGCTTGTTAGGACATGAATCACTGTGGTCCAATGCGGAAAATCAAACTCTGAAAACGATTAGAGTTCCTGGGGTGACTCATACTGAGATACTCAAAGATGATGGTGCACTTGGTGAAGTGGTTAGAGAAATCTCTGGAATTAATTCTCAGTTCATGTTTGTTGAGAATTTGGGAGGACGAGAAAAAAATGTTGGACCGAGTGGGAAGAAGAACGTGCAAAGTGGATTGCCACATCCCACATGCAAGTTCGTGCACTTTTGCATTGGATTGCATTGCAACATCATTTTCCCCCTTTTGAATATTGACTGA

Protein Analysis

341

Amino Acids

37.55

Weight (kDa)

7.15

Isoelectric Point (pI)

39.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LCAT PF02450 4 - 172 5.5e-32 Lecithin:cholesterol acyltransferase
Hydrolase_4 PF12146 41 - 101 5e-07 Serine aminopeptidase, S33
Abhydrolase_6 PF12697 44 - 171 2.5e-06 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015278)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G27480
fragaria_vesca FvH4_2g32820 FvH4_2g32820
prunus_persica Prupe.1G420700_v2.0.a1
pyrus_communis pycom08g06610
rosa_chinensis RchiOBHm_Chr6g0309881
rosa_laevigata RLG00000010527
rosa_multiflora Rmu_sc0006780.1_g000001
rosa_roxburghii Rroxscaffold_7G00159080
rosa_rugosa Rorug06G0375600
rosa_samantha Rh6AG489300 Rh6AG489400 Rh6BG498800 Rh6CG503600 Rh6DG489700
rosa_wichuraiana Rw6G042590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 4, 258, 592
AccII CGCG 1 cut(s) 553
AciI CCGC 1 cut(s) 752
AclWI GGATC 1 cut(s) 579
AcsI RAATTY 2 cut(s) 534, 883
AcuI CTGAAG 1 cut(s) 321
AcyI GRCGYC 1 cut(s) 259
AdeI CACNNNGTG 1 cut(s) 830
AfiI CCNNNNNNNGG 2 cut(s) 305, 624
AflIII ACRYGT 2 cut(s) 277, 711
AgeI ACCGGT 1 cut(s) 617
AgsI TTSAA 3 cut(s) 547, 581, 1015
AjnI CCWGG 2 cut(s) 586, 784
AjuI GAANNNNNNNTTGG 2 cut(s) 891, 923
AluBI AGCT 7 cut(s) 65, 207, 399, 488, 577, 584, 720
AluI AGCT 7 cut(s) 65, 207, 399, 488, 577, 584, 720
Alw21I GWGCWC 4 cut(s) 67, 401, 826, 972
Alw26I GTCTC 2 cut(s) 202, 629
Alw44I GTGCAC 2 cut(s) 822, 968
AlwI GGATC 1 cut(s) 579
AoxI GGCC 2 cut(s) 159, 214
ApaLI GTGCAC 2 cut(s) 822, 968
ApoI RAATTY 2 cut(s) 534, 883
AseI ATTAAT 1 cut(s) 858
AsiGI ACCGGT 1 cut(s) 617
Asp700I GAANNNNTTC 2 cut(s) 534, 859
AspLEI GCGC 2 cut(s) 261, 555
AspS9I GGNCC 3 cut(s) 615, 744, 911
AsuHPI GGTGA 5 cut(s) 199, 319, 457, 802, 842
AvaII GGWCC 3 cut(s) 615, 744, 911
BaeGI GKGCMC 2 cut(s) 826, 972
BanI GGYRCC 3 cut(s) 4, 258, 592
BanII GRGCYC 2 cut(s) 67, 401
Bbv12I GWGCWC 4 cut(s) 67, 401, 826, 972
BccI CCATC 6 cut(s) 38, 115, 562, 686, 692, 812
BciT130I CCWGG 2 cut(s) 588, 786
BclI TGATCA 1 cut(s) 462
BcoDI GTCTC 2 cut(s) 202, 629
BfaI CTAG 3 cut(s) 11, 350, 432
BfoI RGCGCY 1 cut(s) 262
Bme1390I CCNGG 2 cut(s) 588, 786
Bme18I GGWCC 3 cut(s) 615, 744, 911
BmgT120I GGNCC 3 cut(s) 615, 744, 911
BmiI GGNNCC 4 cut(s) 6, 260, 594, 616
BmrFI CCNGG 2 cut(s) 588, 786
BmsI GCATC 1 cut(s) 463
Bpu10I CCTNAGC 1 cut(s) 208
BsaHI GRCGYC 1 cut(s) 259
BsaJI CCNNGG 3 cut(s) 299, 587, 785
BsaWI WCCGGW 2 cut(s) 617, 673
Bsc4I CCNNNNNNNGG 2 cut(s) 305, 624
Bse118I RCCGGY 3 cut(s) 161, 184, 617
Bse3DI GCAATG 2 cut(s) 371, 988
BseBI CCWGG 2 cut(s) 588, 786
BseDI CCNNGG 3 cut(s) 299, 587, 785
BseGI GGATG 4 cut(s) 107, 538, 697, 949
BseLI CCNNNNNNNGG 2 cut(s) 305, 624
BseMI GCAATG 2 cut(s) 371, 988
BseMII CTCAG 3 cut(s) 306, 792, 878
BseRI GAGGAG 1 cut(s) 409
BseSI GKGCMC 2 cut(s) 826, 972
Bsh1236I CGCG 1 cut(s) 553
BshFI GGCC 2 cut(s) 161, 216
BshNI GGYRCC 3 cut(s) 4, 258, 592
BshTI ACCGGT 1 cut(s) 617
BsiHKAI GWGCWC 4 cut(s) 67, 401, 826, 972
BsiSI CCGG 4 cut(s) 162, 185, 618, 674
BslFI GGGAC 2 cut(s) 322, 628
BslI CCNNNNNNNGG 2 cut(s) 305, 624
BsmAI GTCTC 2 cut(s) 202, 629
BsmFI GGGAC 2 cut(s) 322, 628
BsnI GGCC 2 cut(s) 161, 216
Bsp1286I GDGCHC 4 cut(s) 67, 401, 826, 972
Bsp143I GATC 2 cut(s) 462, 571
BspACI CCGC 1 cut(s) 752
BspANI GGCC 2 cut(s) 161, 216
BspCNI CTCAG 3 cut(s) 305, 793, 877
BspFNI CGCG 1 cut(s) 553
BspLI GGNNCC 4 cut(s) 6, 260, 594, 616
BspPI GGATC 1 cut(s) 579
BspQI GCTCTTC 1 cut(s) 381
BspT107I GGYRCC 3 cut(s) 4, 258, 592
BsrDI GCAATG 2 cut(s) 371, 988
BsrFI RCCGGY 3 cut(s) 161, 184, 617
BssAI RCCGGY 3 cut(s) 161, 184, 617
BssECI CCNNGG 3 cut(s) 299, 587, 785
BssMI GATC 2 cut(s) 462, 571
BssNI GRCGYC 1 cut(s) 259
Bst2UI CCWGG 2 cut(s) 588, 786
Bst4CI ACNGT 4 cut(s) 300, 313, 706, 741
Bst6I CTCTTC 1 cut(s) 381
BstACI GRCGYC 1 cut(s) 259
BstC8I GCNNGC 1 cut(s) 163
BstDEI CTNAG 5 cut(s) 123, 208, 292, 801, 864
BstDSI CCRYGG 1 cut(s) 299
BstF5I GGATG 4 cut(s) 107, 538, 697, 949
BstFNI CGCG 1 cut(s) 553
BstH2I RGCGCY 1 cut(s) 262
BstHHI GCGC 2 cut(s) 261, 555
BstKTI GATC 2 cut(s) 465, 574
BstMAI GTCTC 2 cut(s) 202, 629
BstMBI GATC 2 cut(s) 462, 571
BstMWI GCNNNNNNNGC 3 cut(s) 213, 293, 405
BstNI CCWGG 2 cut(s) 588, 786
BstNSI RCATGY 2 cut(s) 715, 960
BstSCI CCNGG 2 cut(s) 586, 784
BstSLI GKGCMC 2 cut(s) 826, 972
BstUI CGCG 1 cut(s) 553
BsuRI GGCC 2 cut(s) 161, 216
BtgI CCRYGG 1 cut(s) 299
BtsCI GGATG 4 cut(s) 107, 538, 697, 949
BtsIMutI CAGTG 1 cut(s) 737
Cac8I GCNNGC 1 cut(s) 163
CfoI GCGC 2 cut(s) 261, 555
Cfr10I RCCGGY 3 cut(s) 161, 184, 617
Cfr13I GGNCC 3 cut(s) 615, 744, 911
CspAI ACCGGT 1 cut(s) 617
CviAII CATG 6 cut(s) 55, 255, 712, 731, 871, 957
DdeI CTNAG 5 cut(s) 123, 208, 292, 801, 864
DinI GGCGCC 1 cut(s) 260
DpnI GATC 2 cut(s) 464, 573
DpnII GATC 2 cut(s) 462, 571
DraIII CACNNNGTG 1 cut(s) 830
Eam1104I CTCTTC 1 cut(s) 381
EarI CTCTTC 1 cut(s) 381
Ecl136II GAGCTC 2 cut(s) 65, 399
Eco147I AGGCCT 1 cut(s) 216
Eco24I GRGCYC 2 cut(s) 67, 401
Eco47I GGWCC 3 cut(s) 615, 744, 911
Eco53kI GAGCTC 2 cut(s) 65, 399
Eco57I CTGAAG 1 cut(s) 321
EcoICRI GAGCTC 2 cut(s) 65, 399
EcoRI GAATTC 1 cut(s) 534
EcoRII CCWGG 2 cut(s) 586, 784
EcoT38I GRGCYC 2 cut(s) 67, 401
EgeI GGCGCC 1 cut(s) 260
EheI GGCGCC 1 cut(s) 260
FaeI CATG 6 cut(s) 58, 258, 715, 734, 874, 960
FaqI GGGAC 2 cut(s) 322, 628
FatI CATG 6 cut(s) 54, 254, 711, 730, 870, 956
FbaI TGATCA 1 cut(s) 462
FokI GGATG 4 cut(s) 94, 525, 704, 936
FriOI GRGCYC 2 cut(s) 67, 401
FspBI CTAG 3 cut(s) 11, 350, 432
GlaI GCGC 2 cut(s) 260, 554
HaeII RGCGCY 1 cut(s) 262
HaeIII GGCC 2 cut(s) 161, 216
HapII CCGG 4 cut(s) 162, 185, 618, 674
HhaI GCGC 2 cut(s) 261, 555
Hin1I GRCGYC 1 cut(s) 259
Hin1II CATG 6 cut(s) 58, 258, 715, 734, 874, 960
Hin6I GCGC 2 cut(s) 259, 553
HinP1I GCGC 2 cut(s) 259, 553
HinfI GANTC 5 cut(s) 17, 238, 367, 734, 793
HpaII CCGG 4 cut(s) 162, 185, 618, 674
HphI GGTGA 5 cut(s) 199, 319, 457, 802, 842
Hpy166II GTNNAC 5 cut(s) 316, 685, 709, 824, 970
Hpy188I TCNGA 4 cut(s) 62, 237, 403, 768
Hpy188III TCNNGA 5 cut(s) 130, 442, 527, 565, 852
Hpy8I GTNNAC 5 cut(s) 316, 685, 709, 824, 970
HpyAV CCTTC 2 cut(s) 524, 553
HpyCH4III ACNGT 4 cut(s) 300, 313, 706, 741
HpyCH4IV ACGT 2 cut(s) 279, 930
HpyCH4V TGCA 8 cut(s) 287, 824, 934, 960, 970, 978, 988, 993
HpyF10VI GCNNNNNNNGC 3 cut(s) 213, 293, 405
HpyF3I CTNAG 5 cut(s) 123, 208, 292, 801, 864
HpySE526I ACGT 2 cut(s) 279, 930
Hsp92I GRCGYC 1 cut(s) 259
Hsp92II CATG 6 cut(s) 58, 258, 715, 734, 874, 960
HspAI GCGC 2 cut(s) 259, 553
KasI GGCGCC 1 cut(s) 258
KroI GCCGGC 1 cut(s) 161
KroNI GCCGGC 1 cut(s) 163
Ksp22I TGATCA 1 cut(s) 462
Kzo9I GATC 2 cut(s) 462, 571
LguI GCTCTTC 1 cut(s) 381
LmnI GCTCC 5 cut(s) 62, 70, 396, 404, 589
LweI GCATC 1 cut(s) 463
MaeI CTAG 3 cut(s) 11, 350, 432
MaeII ACGT 2 cut(s) 279, 930
MaeIII GTNAC 1 cut(s) 790
MalI GATC 2 cut(s) 464, 573
MboI GATC 2 cut(s) 462, 571
MboII GAAGA 3 cut(s) 398, 934, 937
MhlI GDGCHC 4 cut(s) 67, 401, 826, 972
MluCI AATT 6 cut(s) 166, 503, 534, 855, 859, 883
Mly113I GGCGCC 1 cut(s) 259
MlyI GAGTC 1 cut(s) 787
MmeI TCCRAC 2 cut(s) 253, 889
MroNI GCCGGC 1 cut(s) 161
MroXI GAANNNNTTC 2 cut(s) 534, 859
MseI TTAA 1 cut(s) 858
MspI CCGG 4 cut(s) 162, 185, 618, 674
MspR9I CCNGG 2 cut(s) 588, 786
MvaI CCWGG 2 cut(s) 588, 786
MvnI CGCG 1 cut(s) 553
MwoI GCNNNNNNNGC 3 cut(s) 213, 293, 405
NaeI GCCGGC 1 cut(s) 163
NarI GGCGCC 1 cut(s) 259
NdeII GATC 2 cut(s) 462, 571
NgoMIV GCCGGC 1 cut(s) 161
NlaIII CATG 6 cut(s) 58, 258, 715, 734, 874, 960
NlaIV GGNNCC 4 cut(s) 6, 260, 594, 616
NmuCI GTSAC 1 cut(s) 790
NspI RCATGY 2 cut(s) 715, 960
PceI AGGCCT 1 cut(s) 216
PciI ACATGT 1 cut(s) 711
PciSI GCTCTTC 1 cut(s) 381
PdiI GCCGGC 1 cut(s) 163
PdmI GAANNNNTTC 2 cut(s) 534, 859
PfeI GAWTC 4 cut(s) 17, 238, 367, 734
PflFI GACNNNGTC 1 cut(s) 149
PinAI ACCGGT 1 cut(s) 617
PleI GAGTC 1 cut(s) 787
PluTI GGCGCC 1 cut(s) 262
PpsI GAGTC 1 cut(s) 787
PscI ACATGT 1 cut(s) 711
PshBI ATTAAT 1 cut(s) 858
Psp124BI GAGCTC 2 cut(s) 67, 401
Psp6I CCWGG 2 cut(s) 586, 784
PspGI CCWGG 2 cut(s) 586, 784
PspN4I GGNNCC 4 cut(s) 6, 260, 594, 616
PspPI GGNCC 3 cut(s) 615, 744, 911
PsyI GACNNNGTC 1 cut(s) 149
SacI GAGCTC 2 cut(s) 67, 401
SapI GCTCTTC 1 cut(s) 381
SaqAI TTAA 1 cut(s) 858
Sau3AI GATC 2 cut(s) 462, 571
Sau96I GGNCC 3 cut(s) 615, 744, 911
SchI GAGTC 1 cut(s) 787
ScrFI CCNGG 2 cut(s) 588, 786
SduI GDGCHC 4 cut(s) 67, 401, 826, 972
SfaNI GCATC 1 cut(s) 463
SfoI GGCGCC 1 cut(s) 260
SinI GGWCC 3 cut(s) 615, 744, 911
Sse9I AATT 6 cut(s) 166, 503, 534, 855, 859, 883
SseBI AGGCCT 1 cut(s) 216
SsiI CCGC 1 cut(s) 752
SspDI GGCGCC 1 cut(s) 258
SspI AATATT 2 cut(s) 31, 1018
SspMI CTAG 3 cut(s) 11, 350, 432
SstI GAGCTC 2 cut(s) 67, 401
StuI AGGCCT 1 cut(s) 216
StyD4I CCNGG 2 cut(s) 586, 784
TaaI ACNGT 4 cut(s) 300, 313, 706, 741
TaiI ACGT 2 cut(s) 282, 933
TaqI TCGA 4 cut(s) 42, 79, 153, 241
TaqII GACCGA 1 cut(s) 928
TasI AATT 6 cut(s) 166, 503, 534, 855, 859, 883
TfiI GAWTC 4 cut(s) 17, 238, 367, 734
Tru1I TTAA 1 cut(s) 858
Tru9I TTAA 1 cut(s) 858
TscAI CASTG 1 cut(s) 744
TseFI GTSAC 1 cut(s) 790
Tsp45I GTSAC 1 cut(s) 790
TspDTI ATGAA 4 cut(s) 259, 527, 747, 859
TspRI CASTG 1 cut(s) 744
Tth111I GACNNNGTC 1 cut(s) 149
VneI GTGCAC 2 cut(s) 822, 968
VpaK11BI GGWCC 3 cut(s) 615, 744, 911
VspI ATTAAT 1 cut(s) 858
XapI RAATTY 2 cut(s) 534, 883
XceI RCATGY 2 cut(s) 715, 960
XmnI GAANNNNTTC 2 cut(s) 534, 859
XspI CTAG 3 cut(s) 11, 350, 432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.